CSPI04G00970 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI04G00970
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	PMEI domain-containing protein
Location	Chr4: 513209 .. 514158 (+)
RNA-Seq Expression	CSPI04G00970
Synteny	CSPI04G00970

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ACCCCTACTCCCATCTCTCCCTCCAAAAAAAATTTGATAGTAATAATAAGAGGAGAAAAAAAGAAAAAAAAAACATTAGGCCATGAAATTTTTTTCAGCCATTTTGTTGGTCCTCTGCCTTTGCATGGCACCTCGGCGCCTGGACGCGGCCGGGGCTGCACAAGAGGAGGGACTAGGCATGATACAGAAAATGTGTGCACAAACTAACTACAAAGACCTTTGCATCACCTCCCTGACTTCTGACCCCAACAGCTTCCCTGCCGACAAGATGGGGCTAGCCTTGGTTGCCCTCCGCCTTGCCTCCTCCAATGCCTCCGACATATCGGAGAGCATCAAGGTGATGCTCAACGAAACGAGCCAAAATAATGAGCCTACAGTACAACAAGCTCTGTTTGATTGCCTTGATGAGTATCTAGAAGCATCTCAACAACTTGACGACTCCATTGCTGCAATTATCGCCAAGGCCTATGGCGACGTGCAGGAATGGGTGCGTGTGGCAGTCACCAATGTGCGCACGTGTGAGAGCTCATTCCCGACGAAGCCGTCTGTGCTGACACCAAGGAACGAGGAGTTTATTAAGCTATGTGACATTGCACTATCCATCACCAAGATTGCAGAAACCAATTAACTATATAAATTTATAGTTTTTTTTTTCTACCTTTTTCATGGAAGATTGGAGCAAAATGGCTGGTTTTGGCTATTCTTTATATGGTGAAGCTTTTTCATTATGTGTTTCTTTTTTCTGTTTGAGATATAGTTCCTCTGTTTAGGATGAGGAGTTGCTAATTGGTGTATTATTATTGTTTCAATGGGGAGGGAGATAATGAAGTTGGACACAAACACAAAACTCTTTTTTAAAAGGTTTTGTGGATAATGGCCGTCTCTATTTTTTTTCATATATTTGAACTTTTGTCTAATTTTATATATTAAAATTGCATTTATATGCCATG

mRNA sequence

ACCCCTACTCCCATCTCTCCCTCCAAAAAAAATTTGATAGTAATAATAAGAGGAGAAAAAAAGAAAAAAAAAACATTAGGCCATGAAATTTTTTTCAGCCATTTTGTTGGTCCTCTGCCTTTGCATGGCACCTCGGCGCCTGGACGCGGCCGGGGCTGCACAAGAGGAGGGACTAGGCATGATACAGAAAATGTGTGCACAAACTAACTACAAAGACCTTTGCATCACCTCCCTGACTTCTGACCCCAACAGCTTCCCTGCCGACAAGATGGGGCTAGCCTTGGTTGCCCTCCGCCTTGCCTCCTCCAATGCCTCCGACATATCGGAGAGCATCAAGGTGATGCTCAACGAAACGAGCCAAAATAATGAGCCTACAGTACAACAAGCTCTGTTTGATTGCCTTGATGAGTATCTAGAAGCATCTCAACAACTTGACGACTCCATTGCTGCAATTATCGCCAAGGCCTATGGCGACGTGCAGGAATGGGTGCGTGTGGCAGTCACCAATGTGCGCACGTGTGAGAGCTCATTCCCGACGAAGCCGTCTGTGCTGACACCAAGGAACGAGGAGTTTATTAAGCTATGTGACATTGCACTATCCATCACCAAGATTGCAGAAACCAATTAACTATATAAATTTATAGTTTTTTTTTTCTACCTTTTTCATGGAAGATTGGAGCAAAATGGCTGGTTTTGGCTATTCTTTATATGGTGAAGCTTTTTCATTATGTGTTTCTTTTTTCTGTTTGAGATATAGTTCCTCTGTTTAGGATGAGGAGTTGCTAATTGGTGTATTATTATTGTTTCAATGGGGAGGGAGATAATGAAGTTGGACACAAACACAAAACTCTTTTTTAAAAGGTTTTGTGGATAATGGCCGTCTCTATTTTTTTTCATATATTTGAACTTTTGTCTAATTTTATATATTAAAATTGCATTTATATGCCATG

Coding sequence (CDS)

ATGAAATTTTTTTCAGCCATTTTGTTGGTCCTCTGCCTTTGCATGGCACCTCGGCGCCTGGACGCGGCCGGGGCTGCACAAGAGGAGGGACTAGGCATGATACAGAAAATGTGTGCACAAACTAACTACAAAGACCTTTGCATCACCTCCCTGACTTCTGACCCCAACAGCTTCCCTGCCGACAAGATGGGGCTAGCCTTGGTTGCCCTCCGCCTTGCCTCCTCCAATGCCTCCGACATATCGGAGAGCATCAAGGTGATGCTCAACGAAACGAGCCAAAATAATGAGCCTACAGTACAACAAGCTCTGTTTGATTGCCTTGATGAGTATCTAGAAGCATCTCAACAACTTGACGACTCCATTGCTGCAATTATCGCCAAGGCCTATGGCGACGTGCAGGAATGGGTGCGTGTGGCAGTCACCAATGTGCGCACGTGTGAGAGCTCATTCCCGACGAAGCCGTCTGTGCTGACACCAAGGAACGAGGAGTTTATTAAGCTATGTGACATTGCACTATCCATCACCAAGATTGCAGAAACCAATTAA

Protein sequence

MKFFSAILLVLCLCMAPRRLDAAGAAQEEGLGMIQKMCAQTNYKDLCITSLTSDPNSFPADKMGLALVALRLASSNASDISESIKVMLNETSQNNEPTVQQALFDCLDEYLEASQQLDDSIAAIIAKAYGDVQEWVRVAVTNVRTCESSFPTKPSVLTPRNEEFIKLCDIALSITKIAETN*

Homology

[Close]

BLAST of CSPI04G00970 vs. ExPASy Swiss-Prot
Match: Q0J8J8 (Pectinesterase inhibitor 28 OS=Oryza sativa subsp. japonica OX=39947 GN=PMEI28 PE=2 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 6.1e-09
Identity = 42/156 (26.92%), Postives = 76/156 (48.72%), Query Frame = 0


Query: 29  EGLGMIQKMCAQTNYKDLCITSLTSDPNSFPADKMGLALVALRLASSNASDISESIKVML 88
           +   +++  C  T Y D+C+++L +DP+S  AD  GL+ +A+ +A++NAS  +++   + 
Sbjct: 57  QAAALVRATCNSTAYYDVCVSALAADPSSTTADVRGLSAIAVSVAAANASGAAQAAAALA 116

Query: 89  NET-----SQNNEPTVQQALFDCLDEYLEASQQLDDSIAAIIAKAYGDVQEWVRVAVTNV 148
           N T     +   + TVQ  L  C  +Y +A   L  +  ++  + Y      V       
Sbjct: 117 NGTAPLAAAAAGDGTVQALLRACAGKYGDARDALAAAKESMGQQDYDLATVHVSAGAEYP 176

Query: 149 RTCESSFPTK-----PSVLTPRNEEFIKLCDIALSI 175
           + C++ F  +     P+ L  R E   KLC +AL I
Sbjct: 177 QVCKALFRRQRPGAYPAELAAREEALNKLCSVALDI 212

[Close]

BLAST of CSPI04G00970 vs. ExPASy TrEMBL
Match: A0A0A0KY96 (PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G001930 PE=4 SV=1)

HSP 1 Score: 349.4 bits (895), Expect = 9.3e-93
Identity = 180/181 (99.45%), Postives = 181/181 (100.00%), Query Frame = 0


Query: 1   MKFFSAILLVLCLCMAPRRLDAAGAAQEEGLGMIQKMCAQTNYKDLCITSLTSDPNSFPA 60
           MKFFSAILLVLCLCMAP+RLDAAGAAQEEGLGMIQKMCAQTNYKDLCITSLTSDPNSFPA
Sbjct: 1   MKFFSAILLVLCLCMAPQRLDAAGAAQEEGLGMIQKMCAQTNYKDLCITSLTSDPNSFPA 60

Query: 61  DKMGLALVALRLASSNASDISESIKVMLNETSQNNEPTVQQALFDCLDEYLEASQQLDDS 120
           DKMGLALVALRLASSNASDISESIKVMLNETSQNNEPTVQQALFDCLDEYLEASQQLDDS
Sbjct: 61  DKMGLALVALRLASSNASDISESIKVMLNETSQNNEPTVQQALFDCLDEYLEASQQLDDS 120

Query: 121 IAAIIAKAYGDVQEWVRVAVTNVRTCESSFPTKPSVLTPRNEEFIKLCDIALSITKIAET 180
           IAAIIAKAYGDVQEWVRVAVTNVRTCESSFPTKPSVLTPRNEEFIKLCDIALSITKIAET
Sbjct: 121 IAAIIAKAYGDVQEWVRVAVTNVRTCESSFPTKPSVLTPRNEEFIKLCDIALSITKIAET 180

Query: 181 N 182
           N
Sbjct: 181 N 181

[Close]

BLAST of CSPI04G00970 vs. ExPASy TrEMBL
Match: A0A5D3E196 (Putative invertase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G001210 PE=4 SV=1)

HSP 1 Score: 277.7 bits (709), Expect = 3.4e-71
Identity = 142/167 (85.03%), Postives = 154/167 (92.22%), Query Frame = 0


Query: 15  MAPRRLDAAGAAQEEGLGMIQKMCAQTNYKDLCITSLTSDPNSFPADKMGLALVALRLAS 74
           MA +RLDAA  A +EG+  I+KMCAQTNYKDLC+TSLTSDPNSFPADKMGLALVALRLAS
Sbjct: 1   MAAQRLDAASQANKEGIEKIEKMCAQTNYKDLCVTSLTSDPNSFPADKMGLALVALRLAS 60

Query: 75  SNASDISESIKVMLNETSQNNEPTVQQALFDCLDEYLEASQQLDDSIAAIIAKAYGDVQE 134
           SNASDISESIKVMLNETSQ NEP VQQ LFDCLDEYL+ASQQLDDSIAAIIAKAYGDV++
Sbjct: 61  SNASDISESIKVMLNETSQ-NEPAVQQGLFDCLDEYLDASQQLDDSIAAIIAKAYGDVEK 120

Query: 135 WVRVAVTNVRTCESSFPTKPSVLTPRNEEFIKLCDIALSITKIAETN 182
           WV  AV +VRTCE+SFPTKPSVLTPRNEEFIKLCDIALSI++IAE N
Sbjct: 121 WVHAAVADVRTCENSFPTKPSVLTPRNEEFIKLCDIALSISRIAEEN 166

[Close]

BLAST of CSPI04G00970 vs. ExPASy TrEMBL
Match: A0A6J1F7F2 (pectinesterase inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111441544 PE=4 SV=1)

HSP 1 Score: 227.3 bits (578), Expect = 5.3e-56
Identity = 118/180 (65.56%), Postives = 150/180 (83.33%), Query Frame = 0


Query: 1   MKFFSAILLVLCLCMAPRRLDAAGAAQEEGLGMIQKMCAQTNYKDLCITSLTSDPNSFPA 60
           MKF SAI++VLCLCMA +R DA     EEG+ +I++MC+QT +K+LC+ SLTSDPNS+PA
Sbjct: 1   MKFVSAIVVVLCLCMAAKRHDATRPV-EEGMALIEEMCSQTKHKELCVDSLTSDPNSYPA 60

Query: 61  DKMGLALVALRLASSNASDISESIKVMLNETSQNNEPTVQQALFDCLDEYLEASQQLDDS 120
           DK GLALVALR ASSNASDISESIKVM N+ S  ++P VQ+AL DCLDEYL+ASQQLDDS
Sbjct: 61  DKTGLALVALRFASSNASDISESIKVMFND-STLSKPMVQEALLDCLDEYLDASQQLDDS 120

Query: 121 IAAIIAKAYGDVQEWVRVAVTNVRTCESSFPT-KPSVLTPRNEEFIKLCDIALSITKIAE 180
           IAA+  KAYGD+++WV +A+T+VRTC +   + +P +LT RNE FIKLC+IAL+I+KIA+
Sbjct: 121 IAALTVKAYGDLEKWVNIAITDVRTCGTELGSERPPLLTERNEVFIKLCEIALTISKIAQ 178

[Close]

BLAST of CSPI04G00970 vs. ExPASy TrEMBL
Match: A0A6J1J9E7 (pectinesterase inhibitor-like OS=Cucurbita maxima OX=3661 GN=LOC111482494 PE=4 SV=1)

HSP 1 Score: 224.6 bits (571), Expect = 3.5e-55
Identity = 116/180 (64.44%), Postives = 150/180 (83.33%), Query Frame = 0


Query: 1   MKFFSAILLVLCLCMAPRRLDAAGAAQEEGLGMIQKMCAQTNYKDLCITSLTSDPNSFPA 60
           MKF SAI+++LCLCMA +R DA     EEG+ +I++MC+QT +K+LC+ SLTSDPNS+PA
Sbjct: 1   MKFISAIVVILCLCMATKRHDATRPV-EEGMTLIEEMCSQTKHKELCVDSLTSDPNSYPA 60

Query: 61  DKMGLALVALRLASSNASDISESIKVMLNETSQNNEPTVQQALFDCLDEYLEASQQLDDS 120
           DK GLALVALR ASSNASDISESIKVM N+ S  ++P VQ+AL DCLDEYL+ASQQLDDS
Sbjct: 61  DKTGLALVALRFASSNASDISESIKVMFND-STLSKPMVQEALLDCLDEYLDASQQLDDS 120

Query: 121 IAAIIAKAYGDVQEWVRVAVTNVRTCESSFPT-KPSVLTPRNEEFIKLCDIALSITKIAE 180
           IAA+  KAYGD+++WV +A+T+VRTC +   + +P +LT RN+ FIKLC+IAL+I+KIA+
Sbjct: 121 IAALTVKAYGDLEKWVNIAMTDVRTCGTELGSERPPLLTERNDVFIKLCEIALTISKIAQ 178

[Close]

BLAST of CSPI04G00970 vs. ExPASy TrEMBL
Match: A0A6J1DPW6 (pectinesterase inhibitor-like OS=Momordica charantia OX=3673 GN=LOC111022446 PE=4 SV=1)

HSP 1 Score: 211.1 bits (536), Expect = 4.0e-51
Identity = 114/178 (64.04%), Postives = 137/178 (76.97%), Query Frame = 0


Query: 6   AILLVLCLCMAPRRLDAAGAAQEEG---LGMIQKMCAQTNYKDLCITSLTSDPNSFPADK 65
           A+L+V+ L +A     AA  A   G   +G+I++ C+QT +  LC++SL SDPNSFPADK
Sbjct: 8   AMLVVVWLSVA-----AAAEAIRPGKPDMGLIEQTCSQTQHPGLCLSSLKSDPNSFPADK 67

Query: 66  MGLALVALRLASSNASDISESIKVMLNETSQNNEPTVQQALFDCLDEYLEASQQLDDSIA 125
            GLALVALRL SSNASDISE IKVMLN+T   ++P VQQ L+DCLDEYL+ASQQLDDSIA
Sbjct: 68  TGLALVALRLVSSNASDISEGIKVMLNDTRLADQPGVQQGLYDCLDEYLDASQQLDDSIA 127

Query: 126 AIIAKAYGDVQEWVRVAVTNVRTCESSFPTKPSVLTPRNEEFIKLCDIALSITKIAET 181
           A++ KAYGDV +WV  AV  VRTCE+SF  KPSVLTPRN+ FIKLC  AL+I  IAET
Sbjct: 128 ALLVKAYGDVDKWVNAAVAAVRTCEASFSVKPSVLTPRNDNFIKLCQNALTIAHIAET 180

[Close]

BLAST of CSPI04G00970 vs. NCBI nr
Match: XP_004152360.2 (cell wall / vacuolar inhibitor of fructosidase 2 [Cucumis sativus] >KGN52826.1 hypothetical protein Csa_015223 [Cucumis sativus])

HSP 1 Score: 349.4 bits (895), Expect = 1.9e-92
Identity = 180/181 (99.45%), Postives = 181/181 (100.00%), Query Frame = 0


Query: 1   MKFFSAILLVLCLCMAPRRLDAAGAAQEEGLGMIQKMCAQTNYKDLCITSLTSDPNSFPA 60
           MKFFSAILLVLCLCMAP+RLDAAGAAQEEGLGMIQKMCAQTNYKDLCITSLTSDPNSFPA
Sbjct: 1   MKFFSAILLVLCLCMAPQRLDAAGAAQEEGLGMIQKMCAQTNYKDLCITSLTSDPNSFPA 60

Query: 61  DKMGLALVALRLASSNASDISESIKVMLNETSQNNEPTVQQALFDCLDEYLEASQQLDDS 120
           DKMGLALVALRLASSNASDISESIKVMLNETSQNNEPTVQQALFDCLDEYLEASQQLDDS
Sbjct: 61  DKMGLALVALRLASSNASDISESIKVMLNETSQNNEPTVQQALFDCLDEYLEASQQLDDS 120

Query: 121 IAAIIAKAYGDVQEWVRVAVTNVRTCESSFPTKPSVLTPRNEEFIKLCDIALSITKIAET 180
           IAAIIAKAYGDVQEWVRVAVTNVRTCESSFPTKPSVLTPRNEEFIKLCDIALSITKIAET
Sbjct: 121 IAAIIAKAYGDVQEWVRVAVTNVRTCESSFPTKPSVLTPRNEEFIKLCDIALSITKIAET 180

Query: 181 N 182
           N
Sbjct: 181 N 181

[Close]

BLAST of CSPI04G00970 vs. NCBI nr
Match: KAA0044390.1 (putative invertase inhibitor [Cucumis melo var. makuwa] >TYK29518.1 putative invertase inhibitor [Cucumis melo var. makuwa])

HSP 1 Score: 277.7 bits (709), Expect = 7.1e-71
Identity = 142/167 (85.03%), Postives = 154/167 (92.22%), Query Frame = 0


Query: 15  MAPRRLDAAGAAQEEGLGMIQKMCAQTNYKDLCITSLTSDPNSFPADKMGLALVALRLAS 74
           MA +RLDAA  A +EG+  I+KMCAQTNYKDLC+TSLTSDPNSFPADKMGLALVALRLAS
Sbjct: 1   MAAQRLDAASQANKEGIEKIEKMCAQTNYKDLCVTSLTSDPNSFPADKMGLALVALRLAS 60

Query: 75  SNASDISESIKVMLNETSQNNEPTVQQALFDCLDEYLEASQQLDDSIAAIIAKAYGDVQE 134
           SNASDISESIKVMLNETSQ NEP VQQ LFDCLDEYL+ASQQLDDSIAAIIAKAYGDV++
Sbjct: 61  SNASDISESIKVMLNETSQ-NEPAVQQGLFDCLDEYLDASQQLDDSIAAIIAKAYGDVEK 120

Query: 135 WVRVAVTNVRTCESSFPTKPSVLTPRNEEFIKLCDIALSITKIAETN 182
           WV  AV +VRTCE+SFPTKPSVLTPRNEEFIKLCDIALSI++IAE N
Sbjct: 121 WVHAAVADVRTCENSFPTKPSVLTPRNEEFIKLCDIALSISRIAEEN 166

[Close]

BLAST of CSPI04G00970 vs. NCBI nr
Match: XP_038906317.1 (cell wall / vacuolar inhibitor of fructosidase 2-like [Benincasa hispida])

HSP 1 Score: 261.9 bits (668), Expect = 4.0e-66
Identity = 135/180 (75.00%), Postives = 156/180 (86.67%), Query Frame = 0


Query: 1   MKFFSAILLVLCLCMAPRRLDAAGAAQEEGLGMIQKMCAQTNYKDLCITSLTSDPNSFPA 60
           MKF SAIL VLCL M  +RLDAA  A E+GL MI  MCAQTNYKDLC+ SLTSDPNSFPA
Sbjct: 1   MKFVSAILFVLCLSMVAQRLDAASPA-EDGLSMIDHMCAQTNYKDLCVISLTSDPNSFPA 60

Query: 61  DKMGLALVALRLASSNASDISESIKVMLNETSQNNEPTVQQALFDCLDEYLEASQQLDDS 120
           DKMGLALVALR ASSNASDISESIKVMLN+TS  N+P+VQQ LFDCLDEYL+ASQQLDD+
Sbjct: 61  DKMGLALVALRFASSNASDISESIKVMLNDTSL-NKPSVQQGLFDCLDEYLDASQQLDDA 120

Query: 121 IAAIIAKAYGDVQEWVRVAVTNVRTCESSFPTKPSVLTPRNEEFIKLCDIALSITKIAET 180
           IAAI+ KAYGDV++WV  A+ +VRTCE+ F TKPS+L P+N+ FIKLC+IALSI+K+A+T
Sbjct: 121 IAAILVKAYGDVEKWVDAAIASVRTCENEFATKPSLLAPKNDVFIKLCEIALSISKVADT 178

[Close]

BLAST of CSPI04G00970 vs. NCBI nr
Match: XP_022934353.1 (pectinesterase inhibitor-like [Cucurbita moschata])

HSP 1 Score: 227.3 bits (578), Expect = 1.1e-55
Identity = 118/180 (65.56%), Postives = 150/180 (83.33%), Query Frame = 0


Query: 1   MKFFSAILLVLCLCMAPRRLDAAGAAQEEGLGMIQKMCAQTNYKDLCITSLTSDPNSFPA 60
           MKF SAI++VLCLCMA +R DA     EEG+ +I++MC+QT +K+LC+ SLTSDPNS+PA
Sbjct: 1   MKFVSAIVVVLCLCMAAKRHDATRPV-EEGMALIEEMCSQTKHKELCVDSLTSDPNSYPA 60

Query: 61  DKMGLALVALRLASSNASDISESIKVMLNETSQNNEPTVQQALFDCLDEYLEASQQLDDS 120
           DK GLALVALR ASSNASDISESIKVM N+ S  ++P VQ+AL DCLDEYL+ASQQLDDS
Sbjct: 61  DKTGLALVALRFASSNASDISESIKVMFND-STLSKPMVQEALLDCLDEYLDASQQLDDS 120

Query: 121 IAAIIAKAYGDVQEWVRVAVTNVRTCESSFPT-KPSVLTPRNEEFIKLCDIALSITKIAE 180
           IAA+  KAYGD+++WV +A+T+VRTC +   + +P +LT RNE FIKLC+IAL+I+KIA+
Sbjct: 121 IAALTVKAYGDLEKWVNIAITDVRTCGTELGSERPPLLTERNEVFIKLCEIALTISKIAQ 178

[Close]

BLAST of CSPI04G00970 vs. NCBI nr
Match: KAG6581280.1 (Pectinesterase inhibitor, partial [Cucurbita argyrosperma subsp. sororia] >KAG7018002.1 Pectinesterase inhibitor, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 225.7 bits (574), Expect = 3.2e-55
Identity = 118/180 (65.56%), Postives = 149/180 (82.78%), Query Frame = 0


Query: 1   MKFFSAILLVLCLCMAPRRLDAAGAAQEEGLGMIQKMCAQTNYKDLCITSLTSDPNSFPA 60
           MKF SAI++VLCLCMA +R DA     EEG+ +I++MC+QT +K+LC+ SLTSDPNS+PA
Sbjct: 1   MKFISAIVVVLCLCMAAKRHDATRPV-EEGMVLIEEMCSQTKHKELCVDSLTSDPNSYPA 60

Query: 61  DKMGLALVALRLASSNASDISESIKVMLNETSQNNEPTVQQALFDCLDEYLEASQQLDDS 120
           DK GLALVALR ASSNASDISESIKVM N+ S  ++P VQ+AL DCLDEYL+ASQQLDDS
Sbjct: 61  DKTGLALVALRFASSNASDISESIKVMFND-STLSKPMVQEALLDCLDEYLDASQQLDDS 120

Query: 121 IAAIIAKAYGDVQEWVRVAVTNVRTCESSF-PTKPSVLTPRNEEFIKLCDIALSITKIAE 180
           IAA+  KAYGD+++WV +A+T+VRTC +     +P +LT RNE FIKLC+IAL+I+KIA+
Sbjct: 121 IAALTVKAYGDLEKWVNIAITDVRTCGTELGRERPPLLTERNEVFIKLCEIALTISKIAQ 178

[Close]

BLAST of CSPI04G00970 vs. TAIR 10
Match: AT1G55770.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 98.2 bits (243), Expect = 7.2e-21
Identity = 56/151 (37.09%), Postives = 93/151 (61.59%), Query Frame = 0


Query: 32  GMIQKMCAQTNYKDLCITSLTSDPNS--FPADKMGLALVALRLASSNASDISESIKVMLN 91
           GMI  +C  ++  +LC++S+TS P S  F      + ++A+  AS+NAS  S  IK  L 
Sbjct: 25  GMISDLCKHSDDPNLCLSSITSRPESGEFAGTSNQIEIIAISAASANASATSSYIKQKL- 84

Query: 92  ETSQNNEPTVQQALFDCLDEYLEASQQLDDSIAAIIAKAYGDVQEWVRVAVTNVRTCESS 151
            ++++ EP ++  L DC  +Y +A +QLDDSI+A++A A+ DV  W+  A++ + +C S+
Sbjct: 85  -SNEDLEPAIEDTLEDCQKDYQDAVEQLDDSISAMLADAHTDVDVWLSAAISAIESCGSA 144

Query: 152 FPTK---PSVLTPRNEEFIKLCDIALSITKI 178
             ++    + L+ RNE F+KLC  AL I K+
Sbjct: 145 LGSRAGNDAELSQRNEVFLKLCKNALMINKM 173

[Close]

BLAST of CSPI04G00970 vs. TAIR 10
Match: AT4G02250.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 89.4 bits (220), Expect = 3.3e-18
Identity = 54/137 (39.42%), Postives = 78/137 (56.93%), Query Frame = 0


Query: 44  KDLCITSLTSDPNSFPADKMGLALVALRLASSNASDISESIKVMLNETSQNNEPTVQQAL 103
           K  C+ SLTS P +  A    L ++AL +ASSNASD S  IK  L +  +N EP ++  L
Sbjct: 23  KAFCVASLTSRPEAATATAPKLGVIALSIASSNASDTSFYIKAKLKQ--KNLEPALEDTL 82

Query: 104 FDCLDEYLEASQQLDDSIAAIIAKAYGDVQEWVRVAVTNVRTCESSFPTK---PSVLTPR 163
            DC   YL+A  QLDDS+AA++  ++ DV  W+  A+++   CE++   +    + L  R
Sbjct: 83  DDCSKNYLDAVAQLDDSLAALMQNSFIDVDIWLNTAISDGEACENALNDRAGNDAELARR 142

Query: 164 NEEFIKLCDIALSITKI 178
           N   +KLC  AL I  I
Sbjct: 143 NTNLLKLCKDALLINTI 157

[Close]

BLAST of CSPI04G00970 vs. TAIR 10
Match: AT3G17152.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 45.8 bits (107), Expect = 4.2e-05
Identity = 37/149 (24.83%), Postives = 71/149 (47.65%), Query Frame = 0


Query: 30  GLGMIQKMCAQTNYKDLCITSLTSDPNSFPADKMGLALVALRLASSNAS-DISESIKVML 89
           G  ++Q++C +  Y+ LCI++L  DP S  ++  GLA ++L   +   +  ++  I V+ 
Sbjct: 30  GSSLVQRLCKRNRYQALCISTLNVDPRSKTSNLQGLASISLDATTKKFNVTLTYYISVLK 89

Query: 90  NETSQNNEPTVQQALFDCLDEYLEASQQLDDSIAA-IIAKAYGDVQEWVRVAVTNVRTCE 149
           N   + +     +    C++EY  A  +   ++ A + AK Y +    ++  V     CE
Sbjct: 90  NVRGRVD----FERYGTCIEEYGAAVDRFLPAVKADLKAKKYPEAMSEMKDVVAKPGYCE 149

Query: 150 SSFPTKPSVLTPRNEEFIKLCDIALSITK 177
             F  + S +T RN+    + D+   I K
Sbjct: 150 DQFAGE-SPVTARNKAVHDIADMTADIIK 173

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BLAST of CSPI04G00970 vs. TAIR 10
Match: AT5G46930.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 45.8 bits (107), Expect = 4.2e-05
Identity = 34/121 (28.10%), Postives = 62/121 (51.24%), Query Frame = 0


Query: 45  DLCITSLTSDPNSFPADKM-GLALVALRLASSNASDISESIKVMLNETSQNNEPTVQQAL 104
           D C+ SL  +P S  A  + GL  V    ++ NA   + S+K M+++  + ++  V++ L
Sbjct: 41  DFCVKSLEENPQSKTAKSLEGLVFV----STKNAVSKTTSLKGMVDKILKEDKYEVERPL 100

Query: 105 FDCLDEYLEASQQLDDSIAAIIAKAYGDVQEWVRVAVTNVRTCESSF-PTKPSVLTPRNE 164
            DCL+ Y EA   L+ S+  + ++ Y      +  A+    +CE+ F   K +V +P  +
Sbjct: 101 LDCLELYTEAIDSLNQSLDTVKSRDYKTATMLMSAAMDAPGSCETKFTKRKKAVKSPFTK 157

The following BLAST results are available for this feature:

BLAST of CSPI04G00970 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q0J8J8	6.1e-09	26.92	Pectinesterase inhibitor 28 OS=Oryza sativa subsp. japonica OX=39947 GN=PMEI28 P...	[more]

BLAST of CSPI04G00970 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KY96	9.3e-93	99.45	PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G001930 PE=4 S...	[more]
A0A5D3E196	3.4e-71	85.03	Putative invertase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca...	[more]
A0A6J1F7F2	5.3e-56	65.56	pectinesterase inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111441544 PE=4...	[more]
A0A6J1J9E7	3.5e-55	64.44	pectinesterase inhibitor-like OS=Cucurbita maxima OX=3661 GN=LOC111482494 PE=4 S...	[more]
A0A6J1DPW6	4.0e-51	64.04	pectinesterase inhibitor-like OS=Momordica charantia OX=3673 GN=LOC111022446 PE=...	[more]

BLAST of CSPI04G00970 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004152360.2	1.9e-92	99.45	cell wall / vacuolar inhibitor of fructosidase 2 [Cucumis sativus] >KGN52826.1 h...	[more]
KAA0044390.1	7.1e-71	85.03	putative invertase inhibitor [Cucumis melo var. makuwa] >TYK29518.1 putative inv...	[more]
XP_038906317.1	4.0e-66	75.00	cell wall / vacuolar inhibitor of fructosidase 2-like [Benincasa hispida]	[more]
XP_022934353.1	1.1e-55	65.56	pectinesterase inhibitor-like [Cucurbita moschata]	[more]
KAG6581280.1	3.2e-55	65.56	Pectinesterase inhibitor, partial [Cucurbita argyrosperma subsp. sororia] >KAG70...	[more]

BLAST of CSPI04G00970 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G55770.1	7.2e-21	37.09	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT4G02250.1	3.3e-18	39.42	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT3G17152.1	4.2e-05	24.83	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G46930.1	4.2e-05	28.10	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 29..174 e-value: 4.1E-30 score: 116.0
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 4..177 e-value: 2.2E-39 score: 132.6
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 34..174 e-value: 2.3E-31 score: 109.2
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 32..177 e-value: 5.7E-39 score: 135.5
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 32..176
None	No IPR available	PANTHER	PTHR31080:SF1	OS05G0360900 PROTEIN	coord: 15..177
None	No IPR available	PANTHER	PTHR31080	PECTINESTERASE INHIBITOR-LIKE	coord: 15..177
None	No IPR available	CDD	cd15801	PMEI-like_1	coord: 33..178 e-value: 1.2673E-48 score: 152.871

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI04G00970.1	CSPI04G00970.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
molecular_function	GO:0030599	pectinesterase activity
molecular_function	GO:0046910	pectinesterase inhibitor activity
molecular_function	GO:0004857	enzyme inhibitor activity