CSPI04G00950 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI04G00950
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Epoxide hydrolase 2
Location	Chr4: 494674 .. 496029 (-)
RNA-Seq Expression	CSPI04G00950
Synteny	CSPI04G00950

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GAAATTGAAAGGGTGTGGAAGTGAATGAATTAGGTAAGGCTCCTGCATGCAAAATTGCTTCCATTATTTAAACCCACATAGAGAATAAACAAAACAAAGCCATCAATGGAGAAAATCCAACACTCAACCATCCCTACCAATGGCATTAACATCCACCTTGCTTCCATTGGCTCCGGCCCCGCGGTGCTATTCCTCCATGGCTTCCCAGAGATCTGGTACACATGGCGTCACCAGCTTCTCTTTTTTGCTTCCAAGGGCTTCCGAGCCATAGCGCCTGATCTCCGTGGATATGGTGACTCCGATGTGCCACCATCTCCTTCTTCCTATACAGCTCACCACATCGTCGGCGATCTCATTGGCCTCCTCGACCATCTCGAAATCGATCAAGTTTTCTTAGTAGGCCACGACTTGGGAGCTACTATAGCTTGGTACTTTTCCATTTTCAGGCCTGATCGAGTCAAGGCTTTGGTCAATTTGAGTGTTTATCATACTCCTCGGATTCCGGAAATGCCGCCTCTCGCCATTATCCGTTTGTTCCTTGGTGATGATTTCTACATTTGTAAATTTCAGGTGAAGGCGATTGAGTCTTTGAACTCTAGCACAAATTCAATTGGGATACATATATCTGATGAGTTTCAATCACATGCATTAATTAAATGTACTGGTTAATAATGAACAGAAAATTGTGAATTTTGAAACAGGAACCTGGAGTCGCAGAAGCAGATTTAGGGTCAGTAGATACGGCTACGATGATGAAGAAATTCCTGACGTTGAGAGATCCAAGCGCTCCGATTGCACCAAACGGATTCAGTACACTACTGGCAACTCCAGAAACCTTACCTTCGTGGTTAACAGAGGAAGATGTAGATTATTACGCTTCCAAATTCGCGAAAACCGGCTTCACCGGCGGACTAAACTACTATCGTGCTCTTGACCTGTGTGGATAGTAACATATTATATTCTTCGTATTTTGAATCAATTGGATTATCTAATTAGAAACCGATGTCGTTTGTGTGCGTGTGTTTTGTTGATTGCTTGTAGAACTTGGGAGCTGACGGGGCCATGGACTGGAGCAAAGATCAAAGTTCCAACAAAATTCATTGTTGGGGATCAAGATCTGGTATACCATTTTCCGGGGGCGAAGGAGTACATTCACGGCGACAGTTTCAAGGAGGACGTACCGCTTTTAGAAGAAGTGGTGGTGATAGAAGGAGCAGCTCACTTCATTAACCAAGAGAAGGCCGATGAAATAAATTCCCTCATCTACGATTTCATCACCAAATTCTAAATAATTCTCACATGGCACACCCCACTACTACTGCTGCACTTACCTCATATGGTCTTATATTTTAGCAC

mRNA sequence

GAAATTGAAAGGGTGTGGAAGTGAATGAATTAGGTAAGGCTCCTGCATGCAAAATTGCTTCCATTATTTAAACCCACATAGAGAATAAACAAAACAAAGCCATCAATGGAGAAAATCCAACACTCAACCATCCCTACCAATGGCATTAACATCCACCTTGCTTCCATTGGCTCCGGCCCCGCGGTGCTATTCCTCCATGGCTTCCCAGAGATCTGGTACACATGGCGTCACCAGCTTCTCTTTTTTGCTTCCAAGGGCTTCCGAGCCATAGCGCCTGATCTCCGTGGATATGGTGACTCCGATGTGCCACCATCTCCTTCTTCCTATACAGCTCACCACATCGTCGGCGATCTCATTGGCCTCCTCGACCATCTCGAAATCGATCAAGTTTTCTTAGTAGGCCACGACTTGGGAGCTACTATAGCTTGGTACTTTTCCATTTTCAGGCCTGATCGAGTCAAGGCTTTGGTCAATTTGAGTGTTTATCATACTCCTCGGATTCCGGAAATGCCGCCTCTCGCCATTATCCGTTTGTTCCTTGGTGATGATTTCTACATTTGTAAATTTCAGGAACCTGGAGTCGCAGAAGCAGATTTAGGGTCAGTAGATACGGCTACGATGATGAAGAAATTCCTGACGTTGAGAGATCCAAGCGCTCCGATTGCACCAAACGGATTCAGTACACTACTGGCAACTCCAGAAACCTTACCTTCGTGGTTAACAGAGGAAGATGTAGATTATTACGCTTCCAAATTCGCGAAAACCGGCTTCACCGGCGGACTAAACTACTATCGTGCTCTTGACCTAACTTGGGAGCTGACGGGGCCATGGACTGGAGCAAAGATCAAAGTTCCAACAAAATTCATTGTTGGGGATCAAGATCTGGTATACCATTTTCCGGGGGCGAAGGAGTACATTCACGGCGACAGTTTCAAGGAGGACGTACCGCTTTTAGAAGAAGTGGTGGTGATAGAAGGAGCAGCTCACTTCATTAACCAAGAGAAGGCCGATGAAATAAATTCCCTCATCTACGATTTCATCACCAAATTCTAAATAATTCTCACATGGCACACCCCACTACTACTGCTGCACTTACCTCATATGGTCTTATATTTTAGCAC

Coding sequence (CDS)

ATGGAGAAAATCCAACACTCAACCATCCCTACCAATGGCATTAACATCCACCTTGCTTCCATTGGCTCCGGCCCCGCGGTGCTATTCCTCCATGGCTTCCCAGAGATCTGGTACACATGGCGTCACCAGCTTCTCTTTTTTGCTTCCAAGGGCTTCCGAGCCATAGCGCCTGATCTCCGTGGATATGGTGACTCCGATGTGCCACCATCTCCTTCTTCCTATACAGCTCACCACATCGTCGGCGATCTCATTGGCCTCCTCGACCATCTCGAAATCGATCAAGTTTTCTTAGTAGGCCACGACTTGGGAGCTACTATAGCTTGGTACTTTTCCATTTTCAGGCCTGATCGAGTCAAGGCTTTGGTCAATTTGAGTGTTTATCATACTCCTCGGATTCCGGAAATGCCGCCTCTCGCCATTATCCGTTTGTTCCTTGGTGATGATTTCTACATTTGTAAATTTCAGGAACCTGGAGTCGCAGAAGCAGATTTAGGGTCAGTAGATACGGCTACGATGATGAAGAAATTCCTGACGTTGAGAGATCCAAGCGCTCCGATTGCACCAAACGGATTCAGTACACTACTGGCAACTCCAGAAACCTTACCTTCGTGGTTAACAGAGGAAGATGTAGATTATTACGCTTCCAAATTCGCGAAAACCGGCTTCACCGGCGGACTAAACTACTATCGTGCTCTTGACCTAACTTGGGAGCTGACGGGGCCATGGACTGGAGCAAAGATCAAAGTTCCAACAAAATTCATTGTTGGGGATCAAGATCTGGTATACCATTTTCCGGGGGCGAAGGAGTACATTCACGGCGACAGTTTCAAGGAGGACGTACCGCTTTTAGAAGAAGTGGTGGTGATAGAAGGAGCAGCTCACTTCATTAACCAAGAGAAGGCCGATGAAATAAATTCCCTCATCTACGATTTCATCACCAAATTCTAA

Protein sequence

MEKIQHSTIPTNGINIHLASIGSGPAVLFLHGFPEIWYTWRHQLLFFASKGFRAIAPDLRGYGDSDVPPSPSSYTAHHIVGDLIGLLDHLEIDQVFLVGHDLGATIAWYFSIFRPDRVKALVNLSVYHTPRIPEMPPLAIIRLFLGDDFYICKFQEPGVAEADLGSVDTATMMKKFLTLRDPSAPIAPNGFSTLLATPETLPSWLTEEDVDYYASKFAKTGFTGGLNYYRALDLTWELTGPWTGAKIKVPTKFIVGDQDLVYHFPGAKEYIHGDSFKEDVPLLEEVVVIEGAAHFINQEKADEINSLIYDFITKF*

Homology

[Close]

BLAST of CSPI04G00950 vs. ExPASy Swiss-Prot
Match: I6YGS0 (Epoxide hydrolase A OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=ephA PE=1 SV=1)

HSP 1 Score: 233.0 bits (593), Expect = 4.6e-60
Identity = 124/319 (38.87%), Postives = 174/319 (54.55%), Query Frame = 0


Query: 9   IPTNGINIHLASIG--SGPAVLFLHGFPEIWYTWRHQLLFFASKGFRAIAPDLRGYGDSD 68
           + TNG+ + +   G    P V+  HGFPE+ Y+WRHQ+   A  G+  +APD RGYG S 
Sbjct: 9   VDTNGVRLRVVEAGEPGAPVVILAHGFPELAYSWRHQIPALADAGYHVLAPDQRGYGGSS 68

Query: 69  VPPSPSSYTAHHIVGDLIGLLDHLEIDQVFLVGHDLGATIAWYFSIFRPDRVKALVNLSV 128
            P +  +Y  H +  DL+GLLD +  ++   VGHD GA + W   +   DRV A+  LSV
Sbjct: 69  RPEAIEAYDIHRLTADLVGLLDDVGAERAVWVGHDWGAVVVWNAPLLHADRVAAVAALSV 128

Query: 129 YHTPRIPEMPPLAIIRLFLGDD-FYICKFQEPGVAEADLGSVDTATMMKKFLTLRDPSAP 188
              PR  ++PP    R   G++ FYI  FQEPG+A+A+L      TM +    LR P   
Sbjct: 129 PALPR-AQVPPTQAFRSRFGENFFYILYFQEPGIADAELNGDPARTMRRMIGGLRPPGDQ 188

Query: 189 IA--------PNGFSTLLATPETLPSWLTEEDVDYYASKFAKTGFTGGLNYYRALDLTWE 248
            A        P+GF   L  P  LP+W+++E++D+Y  +F +TGFTGGLN+YR  D  WE
Sbjct: 189 SAAMRMLAPGPDGFIDRLPEPAGLPAWISQEELDHYIGEFTRTGFTGGLNWYRNFDRNWE 248

Query: 249 LTGPWTGAKIKVPTKFIVGDQDLVYHF---PGAKEYIHGDSFKEDVPLLEEVVVIEGAAH 308
            T    G  I VP+ FI G  D V  F     A E I G       P  E  V+I+GA H
Sbjct: 249 TTADLAGKTISVPSLFIAGTADPVLTFTRTDRAAEVISG-------PYRE--VLIDGAGH 308

Query: 309 FINQEKADEINSLIYDFIT 314
           ++ QE+  E+ + + +F+T
Sbjct: 309 WLQQERPGEVTAALLEFLT 317

[Close]

BLAST of CSPI04G00950 vs. ExPASy Swiss-Prot
Match: P34914 (Bifunctional epoxide hydrolase 2 OS=Mus musculus OX=10090 GN=Ephx2 PE=1 SV=2)

HSP 1 Score: 192.2 bits (487), Expect = 8.9e-48
Identity = 103/306 (33.66%), Postives = 165/306 (53.92%), Query Frame = 0


Query: 13  GINIHLASIGSGPAVLFLHGFPEIWYTWRHQLLFFASKGFRAIAPDLRGYGDSDVPPSPS 72
           GI +H   +GSGPA+   HGFPE W++WR+Q+   A  GFR +A D++GYGDS  PP   
Sbjct: 245 GIRLHFVEMGSGPALCLCHGFPESWFSWRYQIPALAQAGFRVLAIDMKGYGDSSSPPEIE 304

Query: 73  SYTAHHIVGDLIGLLDHLEIDQVFLVGHDLGATIAWYFSIFRPDRVKALVNLSVYHTPRI 132
            Y    +  +++  LD L I Q   +GHD    + W  ++F P+RV+A+ +L+    P  
Sbjct: 305 EYAMELLCKEMVTFLDKLGIPQAVFIGHDWAGVMVWNMALFYPERVRAVASLNTPFMPPD 364

Query: 133 PEMPPLAIIRLFLGDDFYICKFQEPGVAEADLGSVDTATMMKKFLTLRDPSAPIAPNGFS 192
           P++ P+ +IR     ++ +  FQEPGVAEA+L   + +   K F    D +  IA +  +
Sbjct: 365 PDVSPMKVIRSIPVFNYQL-YFQEPGVAEAEL-EKNMSRTFKSFFRASDETGFIAVHKAT 424

Query: 193 ----TLLATPE--TLPSWLTEEDVDYYASKFAKTGFTGGLNYYRALDLTWELTGPWTGAK 252
                L+ TPE   L    TEE++++Y  +F KTGF G LN+YR  +  W+ +    G K
Sbjct: 425 EIGGILVNTPEDPNLSKITTEEEIEFYIQQFKKTGFRGPLNWYRNTERNWKWSCKGLGRK 484

Query: 253 IKVPTKFIVGDQDLVYHFPGAKEYIHGDSFKEDVPLLEEVVVIEGAAHFINQEKADEINS 312
           I VP   +  ++D+V     +K      + ++ +P L+    IE   H+   EK  E+N 
Sbjct: 485 ILVPALMVTAEKDIVLRPEMSK------NMEKWIPFLKR-GHIEDCGHWTQIEKPTEVNQ 541

[Close]

BLAST of CSPI04G00950 vs. ExPASy Swiss-Prot
Match: Q6Q2C2 (Bifunctional epoxide hydrolase 2 OS=Sus scrofa OX=9823 GN=EPHX2 PE=2 SV=1)

HSP 1 Score: 191.8 bits (486), Expect = 1.2e-47
Identity = 102/306 (33.33%), Postives = 167/306 (54.58%), Query Frame = 0


Query: 13  GINIHLASIGSGPAVLFLHGFPEIWYTWRHQLLFFASKGFRAIAPDLRGYGDSDVPPSPS 72
           G+ +H   +GSGPAV   HGFPE W++WR+Q+   A  GFR +A D++GYG+S  PP   
Sbjct: 247 GVRLHFVEMGSGPAVCLCHGFPESWFSWRYQIPALAQAGFRVLAVDMKGYGESSAPPEIE 306

Query: 73  SYTAHHIVGDLIGLLDHLEIDQVFLVGHDLGATIAWYFSIFRPDRVKALVNLSVYHTPRI 132
            Y+   +  D++  L+ L + Q   +GHD G  + W  ++F P+RV+A+ +L+    P  
Sbjct: 307 EYSLEVLCKDMVTFLNKLGLSQAVFIGHDWGGVLVWNMALFYPERVRAVASLNTPFMPSN 366

Query: 133 PEMPPLAIIRLFLGDDFYICKFQEPGVAEADLGSVDTATMMKKFLTLRDPSAPIAPNGFS 192
           P + P+ II+     D+ +  FQEPGVAEA+L   +     K F    D +  +  N   
Sbjct: 367 PNVSPMEIIKANPVFDYQL-YFQEPGVAEAEL-EQNLDRTFKNFFRAHDETF-LTTNRVR 426

Query: 193 TL----LATPE--TLPSWLTEEDVDYYASKFAKTGFTGGLNYYRALDLTWELTGPWTGAK 252
            L    + TPE  +L   +TEED+ +Y  +F K+GF G LN+YR ++  W+     +G K
Sbjct: 427 ELGGLFVGTPEEPSLSRLVTEEDIQFYVQQFKKSGFRGPLNWYRNMERNWQWGCKGSGRK 486

Query: 253 IKVPTKFIVGDQDLVYHFPGAKEYIHGDSFKEDVPLLEEVVVIEGAAHFINQEKADEINS 312
           I +P   +  + DLV H   +K   H +++   +P L+    I+   H+   +K  E+N 
Sbjct: 487 ILIPALMVTAENDLVLHPKMSK---HMENW---IPHLKR-GHIKDCGHWTQIDKPAELNR 542

[Close]

BLAST of CSPI04G00950 vs. ExPASy Swiss-Prot
Match: P80299 (Bifunctional epoxide hydrolase 2 OS=Rattus norvegicus OX=10116 GN=Ephx2 PE=1 SV=1)

HSP 1 Score: 189.9 bits (481), Expect = 4.4e-47
Identity = 101/306 (33.01%), Postives = 162/306 (52.94%), Query Frame = 0


Query: 13  GINIHLASIGSGPAVLFLHGFPEIWYTWRHQLLFFASKGFRAIAPDLRGYGDSDVPPSPS 72
           GI +H   +GSGPA+   HGFPE W++WR+Q+   A  GFR +A D++GYGDS  PP   
Sbjct: 245 GIRLHFVEMGSGPAICLCHGFPESWFSWRYQIPALAQAGFRVLAIDMKGYGDSSSPPEIE 304

Query: 73  SYTAHHIVGDLIGLLDHLEIDQVFLVGHDLGATIAWYFSIFRPDRVKALVNLSVYHTPRI 132
            Y    +  +++  L+ L I Q   +GHD    + W  ++F P+RV+A+ +L+    P  
Sbjct: 305 EYAMELLCEEMVTFLNKLGIPQAVFIGHDWAGVLVWNMALFHPERVRAVASLNTPLMPPN 364

Query: 133 PEMPPLAIIRLFLGDDFYICKFQEPGVAEADLGSVDTATMMKKFLTLRDPSAPIAPNGFS 192
           PE+ P+ +IR     ++ +  FQEPGVAEA+L   + +   K F    D    +  N  +
Sbjct: 365 PEVSPMEVIRSIPVFNYQL-YFQEPGVAEAEL-EKNMSRTFKSFFRTSDDMGLLTVNKAT 424

Query: 193 ----TLLATPE--TLPSWLTEEDVDYYASKFAKTGFTGGLNYYRALDLTWELTGPWTGAK 252
                L+ TPE   +    TEE+++YY  +F K+GF G LN+YR  +  W+ +    G K
Sbjct: 425 EMGGILVGTPEDPKVSKITTEEEIEYYIQQFKKSGFRGPLNWYRNTERNWKWSCKALGRK 484

Query: 253 IKVPTKFIVGDQDLVYHFPGAKEYIHGDSFKEDVPLLEEVVVIEGAAHFINQEKADEINS 312
           I VP   +  ++D+V     +K      + +  +P L+    IE   H+   EK  E+N 
Sbjct: 485 ILVPALMVTAEKDIVLRPEMSK------NMENWIPFLKR-GHIEDCGHWTQIEKPAEVNQ 541

[Close]

BLAST of CSPI04G00950 vs. ExPASy Swiss-Prot
Match: P34913 (Bifunctional epoxide hydrolase 2 OS=Homo sapiens OX=9606 GN=EPHX2 PE=1 SV=2)

HSP 1 Score: 186.0 bits (471), Expect = 6.4e-46
Identity = 98/305 (32.13%), Postives = 161/305 (52.79%), Query Frame = 0


Query: 14  INIHLASIGSGPAVLFLHGFPEIWYTWRHQLLFFASKGFRAIAPDLRGYGDSDVPPSPSS 73
           + +H   +GSGPAV   HGFPE WY+WR+Q+   A  G+R +A D++GYG+S  PP    
Sbjct: 248 VRLHFVELGSGPAVCLCHGFPESWYSWRYQIPALAQAGYRVLAMDMKGYGESSAPPEIEE 307

Query: 74  YTAHHIVGDLIGLLDHLEIDQVFLVGHDLGATIAWYFSIFRPDRVKALVNLSVYHTPRIP 133
           Y    +  +++  LD L + Q   +GHD G  + WY ++F P+RV+A+ +L+    P  P
Sbjct: 308 YCMEVLCKEMVTFLDKLGLSQAVFIGHDWGGMLVWYMALFYPERVRAVASLNTPFIPANP 367

Query: 134 EMPPLAIIRLFLGDDFYICKFQEPGVAEADLGSVDTATMMKKFLTLRDPSA----PIAPN 193
            M PL  I+     D+ +  FQEPGVAEA+L   + +   K      D S      +   
Sbjct: 368 NMSPLESIKANPVFDYQL-YFQEPGVAEAEL-EQNLSRTFKSLFRASDESVLSMHKVCEA 427

Query: 194 GFSTLLATPE--TLPSWLTEEDVDYYASKFAKTGFTGGLNYYRALDLTWELTGPWTGAKI 253
           G    + +PE  +L   +TEE++ +Y  +F K+GF G LN+YR ++  W+      G KI
Sbjct: 428 G-GLFVNSPEEPSLSRMVTEEEIQFYVQQFKKSGFRGPLNWYRNMERNWKWACKSLGRKI 487

Query: 254 KVPTKFIVGDQDLVYHFPGAKEYIHGDSFKEDVPLLEEVVVIEGAAHFINQEKADEINSL 313
            +P   +  ++D V   P   +++     ++ +P L+    IE   H+   +K  E+N +
Sbjct: 488 LIPALMVTAEKDFVL-VPQMSQHM-----EDWIPHLKR-GHIEDCGHWTQMDKPTEVNQI 542

[Close]

BLAST of CSPI04G00950 vs. ExPASy TrEMBL
Match: A0A0A0KWK9 (Epoxide hydrolase 2 OS=Cucumis sativus OX=3659 GN=Csa_4G001890 PE=4 SV=1)

HSP 1 Score: 654.4 bits (1687), Expect = 2.3e-184
Identity = 311/315 (98.73%), Postives = 314/315 (99.68%), Query Frame = 0


Query: 1   MEKIQHSTIPTNGINIHLASIGSGPAVLFLHGFPEIWYTWRHQLLFFASKGFRAIAPDLR 60
           MEKIQHSTIPTNGINIHLASIGSGPA+LFLHGFPEIWYTWRHQLLFFAS+GFRAIAPDLR
Sbjct: 1   MEKIQHSTIPTNGINIHLASIGSGPALLFLHGFPEIWYTWRHQLLFFASRGFRAIAPDLR 60

Query: 61  GYGDSDVPPSPSSYTAHHIVGDLIGLLDHLEIDQVFLVGHDLGATIAWYFSIFRPDRVKA 120
           GYGDSDVPPSPSSYTAHHIVGDLIGLLDHLEIDQVFLVGHDLGATIAWYFSIFRPDRVKA
Sbjct: 61  GYGDSDVPPSPSSYTAHHIVGDLIGLLDHLEIDQVFLVGHDLGATIAWYFSIFRPDRVKA 120

Query: 121 LVNLSVYHTPRIPEMPPLAIIRLFLGDDFYICKFQEPGVAEADLGSVDTATMMKKFLTLR 180
           LVNLSVYHTPRIPEMPPLAIIRLFLGDDFYICKFQEPGVAEADLGSVDTATMMKKFLTLR
Sbjct: 121 LVNLSVYHTPRIPEMPPLAIIRLFLGDDFYICKFQEPGVAEADLGSVDTATMMKKFLTLR 180

Query: 181 DPSAPIAPNGFSTLLATPETLPSWLTEEDVDYYASKFAKTGFTGGLNYYRALDLTWELTG 240
           DPSAPIAPNGFSTLLATPETLPSWLTE+DVDYYASKFAKTGFTGGLNYYRALDLTWELTG
Sbjct: 181 DPSAPIAPNGFSTLLATPETLPSWLTEDDVDYYASKFAKTGFTGGLNYYRALDLTWELTG 240

Query: 241 PWTGAKIKVPTKFIVGDQDLVYHFPGAKEYIHGDSFKEDVPLLEEVVVIEGAAHFINQEK 300
           PWTGAKIKVPTKFIVGDQDLVYHFPGAKEYIHGDSFKEDVP LEEVVVIEGAAHFINQEK
Sbjct: 241 PWTGAKIKVPTKFIVGDQDLVYHFPGAKEYIHGDSFKEDVPHLEEVVVIEGAAHFINQEK 300

Query: 301 ADEINSLIYDFITKF 316
           ADEINSLIYDFITKF
Sbjct: 301 ADEINSLIYDFITKF 315

[Close]

BLAST of CSPI04G00950 vs. ExPASy TrEMBL
Match: A0A5A7TQJ9 (Bifunctional epoxide hydrolase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G001150 PE=4 SV=1)

HSP 1 Score: 565.1 bits (1455), Expect = 1.9e-157
Identity = 263/315 (83.49%), Postives = 285/315 (90.48%), Query Frame = 0


Query: 1   MEKIQHSTIPTNGINIHLASIGSGPAVLFLHGFPEIWYTWRHQLLFFASKGFRAIAPDLR 60
           M+KIQHSTI TNGINIH ASIGSGP VLFLHGFPE+WY+WRHQLLF ASKGFRAIAPDLR
Sbjct: 1   MDKIQHSTISTNGINIHFASIGSGPVVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR 60

Query: 61  GYGDSDVPPSPSSYTAHHIVGDLIGLLDHLEIDQVFLVGHDLGATIAWYFSIFRPDRVKA 120
           G+GDSD PPSPSSYT HHIVGDLIGLLDHL IDQVFLVGHD GA +AWYF +FRPDRVKA
Sbjct: 61  GFGDSDAPPSPSSYTPHHIVGDLIGLLDHLGIDQVFLVGHDWGAMMAWYFCLFRPDRVKA 120

Query: 121 LVNLSVYHTPRIPEMPPLAIIRLFLGDDFYICKFQEPGVAEADLGSVDTATMMKKFLTLR 180
           LVNLSV++TPR P   PLA+ R +LGDDFYICKFQEPGVAEAD GSVDTATMMKKFLT+R
Sbjct: 121 LVNLSVHYTPRNPAGSPLAVTRRYLGDDFYICKFQEPGVAEADFGSVDTATMMKKFLTMR 180

Query: 181 DPSAPIAPNGFSTLLATPETLPSWLTEEDVDYYASKFAKTGFTGGLNYYRALDLTWELTG 240
           DP   I PNGF TLL TPE LPSWLTEED++Y+ASKF+KTGFTGG NYYRALD+TWELTG
Sbjct: 181 DPRPAIIPNGFKTLLETPEILPSWLTEEDIEYFASKFSKTGFTGGFNYYRALDITWELTG 240

Query: 241 PWTGAKIKVPTKFIVGDQDLVYHFPGAKEYIHGDSFKEDVPLLEEVVVIEGAAHFINQEK 300
           PW+ A+IKVPTKFIVGD DLVY+FPGAKEYIHG  FK+DVPLLE+VVVIEGAAHFINQEK
Sbjct: 241 PWSRAQIKVPTKFIVGDLDLVYNFPGAKEYIHGGGFKKDVPLLEDVVVIEGAAHFINQEK 300

Query: 301 ADEINSLIYDFITKF 316
            DEI+SLIYDFITKF
Sbjct: 301 PDEISSLIYDFITKF 315

[Close]

BLAST of CSPI04G00950 vs. ExPASy TrEMBL
Match: A0A1S3BZ44 (bifunctional epoxide hydrolase 2-like OS=Cucumis melo OX=3656 GN=LOC103494761 PE=4 SV=1)

HSP 1 Score: 565.1 bits (1455), Expect = 1.9e-157
Identity = 263/315 (83.49%), Postives = 285/315 (90.48%), Query Frame = 0


Query: 1   MEKIQHSTIPTNGINIHLASIGSGPAVLFLHGFPEIWYTWRHQLLFFASKGFRAIAPDLR 60
           M+KIQHSTI TNGINIH ASIGSGP VLFLHGFPE+WY+WRHQLLF ASKGFRAIAPDLR
Sbjct: 1   MDKIQHSTISTNGINIHFASIGSGPVVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR 60

Query: 61  GYGDSDVPPSPSSYTAHHIVGDLIGLLDHLEIDQVFLVGHDLGATIAWYFSIFRPDRVKA 120
           G+GDSD PPSPSSYT HHIVGDLIGLLDHL IDQVFLVGHD GA +AWYF +FRPDRVKA
Sbjct: 61  GFGDSDAPPSPSSYTPHHIVGDLIGLLDHLGIDQVFLVGHDWGAMMAWYFCLFRPDRVKA 120

Query: 121 LVNLSVYHTPRIPEMPPLAIIRLFLGDDFYICKFQEPGVAEADLGSVDTATMMKKFLTLR 180
           LVNLSV++TPR P   PLA+ R +LGDDFYICKFQEPGVAEAD GSVDTATMMKKFLT+R
Sbjct: 121 LVNLSVHYTPRNPAGSPLAVTRRYLGDDFYICKFQEPGVAEADFGSVDTATMMKKFLTMR 180

Query: 181 DPSAPIAPNGFSTLLATPETLPSWLTEEDVDYYASKFAKTGFTGGLNYYRALDLTWELTG 240
           DP   I PNGF TLL TPE LPSWLTEED++Y+ASKF+KTGFTGG NYYRALD+TWELTG
Sbjct: 181 DPRPAIIPNGFKTLLETPEILPSWLTEEDIEYFASKFSKTGFTGGFNYYRALDITWELTG 240

Query: 241 PWTGAKIKVPTKFIVGDQDLVYHFPGAKEYIHGDSFKEDVPLLEEVVVIEGAAHFINQEK 300
           PW+ A+IKVPTKFIVGD DLVY+FPGAKEYIHG  FK+DVPLLE+VVVIEGAAHFINQEK
Sbjct: 241 PWSRAQIKVPTKFIVGDLDLVYNFPGAKEYIHGGGFKKDVPLLEDVVVIEGAAHFINQEK 300

Query: 301 ADEINSLIYDFITKF 316
            DEI+SLIYDFITKF
Sbjct: 301 PDEISSLIYDFITKF 315

[Close]

BLAST of CSPI04G00950 vs. ExPASy TrEMBL
Match: A0A5D3E108 (Bifunctional epoxide hydrolase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G001170 PE=4 SV=1)

HSP 1 Score: 552.4 bits (1422), Expect = 1.3e-153
Identity = 263/337 (78.04%), Postives = 285/337 (84.57%), Query Frame = 0


Query: 1   MEKIQHSTIPTNGINIHLASIGSGPAVLFLHGFPEIWYTWRHQLLFFASKGFRAIAPDLR 60
           M+KIQHSTI TNGINIH ASIGSGP VLFLHGFPE+WY+WRHQLLF ASKGFRAIAPDLR
Sbjct: 1   MDKIQHSTISTNGINIHFASIGSGPVVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR 60

Query: 61  GYGDSDVPPSPSSYTAHHIVGDLIGLLDHLEIDQVFLVGHDLGATIAWYFSIFRPDRVKA 120
           G+GDSD PPSPSSYT HHIVGDLIGLLDHL IDQVFLVGHD GA +AWYF +FRPDRVKA
Sbjct: 61  GFGDSDAPPSPSSYTPHHIVGDLIGLLDHLGIDQVFLVGHDWGAMMAWYFCLFRPDRVKA 120

Query: 121 LVNLSVYHTPRIPEMPPLAIIRLFLGDDFYICKFQ----------------------EPG 180
           LVNLSV++TPR P   PLA+ R +LGDDFYICKFQ                      EPG
Sbjct: 121 LVNLSVHYTPRNPAGSPLAVTRRYLGDDFYICKFQVNAVDSLNSSVISEIEWLRSHWEPG 180

Query: 181 VAEADLGSVDTATMMKKFLTLRDPSAPIAPNGFSTLLATPETLPSWLTEEDVDYYASKFA 240
           VAEAD GSVDTATMMKKFLT+RDP   I PNGF TLL TPE LPSWLTEED++Y+ASKF+
Sbjct: 181 VAEADFGSVDTATMMKKFLTMRDPRPAIIPNGFKTLLETPEILPSWLTEEDIEYFASKFS 240

Query: 241 KTGFTGGLNYYRALDLTWELTGPWTGAKIKVPTKFIVGDQDLVYHFPGAKEYIHGDSFKE 300
           KTGFTGG NYYRALD+TWELTGPW+ A+IKVPTKFIVGD DLVY+FPGAKEYIHG  FK+
Sbjct: 241 KTGFTGGFNYYRALDITWELTGPWSRAQIKVPTKFIVGDLDLVYNFPGAKEYIHGGGFKK 300

Query: 301 DVPLLEEVVVIEGAAHFINQEKADEINSLIYDFITKF 316
           DVPLLE+VVVIEGAAHFINQEK DEI+SLIYDFITKF
Sbjct: 301 DVPLLEDVVVIEGAAHFINQEKPDEISSLIYDFITKF 337

[Close]

BLAST of CSPI04G00950 vs. ExPASy TrEMBL
Match: A0A6J1F1K3 (uncharacterized protein LOC111441542 OS=Cucurbita moschata OX=3662 GN=LOC111441542 PE=4 SV=1)

HSP 1 Score: 550.8 bits (1418), Expect = 3.7e-153
Identity = 255/315 (80.95%), Postives = 283/315 (89.84%), Query Frame = 0


Query: 1   MEKIQHSTIPTNGINIHLASIGSGPAVLFLHGFPEIWYTWRHQLLFFASKGFRAIAPDLR 60
           MEKI+HSTI TNGIN+H+ASIGSGP VLFLHGFPE+WY+WRHQLLF ASKGFRAIAPDLR
Sbjct: 1   MEKIEHSTIATNGINMHVASIGSGPPVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR 60

Query: 61  GYGDSDVPPSPSSYTAHHIVGDLIGLLDHLEIDQVFLVGHDLGATIAWYFSIFRPDRVKA 120
           G+GDSDVPPSPSSYT  HI+GDLIGLLDHL I+QVFLVGHD GA +AWYF +FRPDRVKA
Sbjct: 61  GFGDSDVPPSPSSYTPFHIIGDLIGLLDHLGIEQVFLVGHDWGAMMAWYFCLFRPDRVKA 120

Query: 121 LVNLSVYHTPRIPEMPPLAIIRLFLGDDFYICKFQEPGVAEADLGSVDTATMMKKFLTLR 180
           LVNLSV++ PR P + PL+  R FLGDDFYICKFQ PGVAEAD GSVDTATMMKKFLT+R
Sbjct: 121 LVNLSVHYNPRNPAISPLSRTRQFLGDDFYICKFQTPGVAEADFGSVDTATMMKKFLTIR 180

Query: 181 DPSAPIAPNGFSTLLATPETLPSWLTEEDVDYYASKFAKTGFTGGLNYYRALDLTWELTG 240
           DPS PI PNGF T L TPETLPSWLTEED+DY+ASKF KTGFTGG NYYRA++ TWELTG
Sbjct: 181 DPSPPIIPNGFKT-LKTPETLPSWLTEEDIDYFASKFTKTGFTGGFNYYRAIEQTWELTG 240

Query: 241 PWTGAKIKVPTKFIVGDQDLVYHFPGAKEYIHGDSFKEDVPLLEEVVVIEGAAHFINQEK 300
           PW+GAKIKVPTK++VGD D+VYH PGAK+YIHG  FK+DVPLLEEVVV+EGAAHFINQEK
Sbjct: 241 PWSGAKIKVPTKYVVGDVDMVYHLPGAKQYIHGGGFKKDVPLLEEVVVMEGAAHFINQEK 300

Query: 301 ADEINSLIYDFITKF 316
           ADEI++ IYDFI KF
Sbjct: 301 ADEISAHIYDFIIKF 314

[Close]

BLAST of CSPI04G00950 vs. NCBI nr
Match: XP_004152244.1 (uncharacterized protein LOC101204815 [Cucumis sativus] >KGN52822.1 hypothetical protein Csa_014596 [Cucumis sativus])

HSP 1 Score: 654.4 bits (1687), Expect = 4.8e-184
Identity = 311/315 (98.73%), Postives = 314/315 (99.68%), Query Frame = 0


Query: 1   MEKIQHSTIPTNGINIHLASIGSGPAVLFLHGFPEIWYTWRHQLLFFASKGFRAIAPDLR 60
           MEKIQHSTIPTNGINIHLASIGSGPA+LFLHGFPEIWYTWRHQLLFFAS+GFRAIAPDLR
Sbjct: 1   MEKIQHSTIPTNGINIHLASIGSGPALLFLHGFPEIWYTWRHQLLFFASRGFRAIAPDLR 60

Query: 61  GYGDSDVPPSPSSYTAHHIVGDLIGLLDHLEIDQVFLVGHDLGATIAWYFSIFRPDRVKA 120
           GYGDSDVPPSPSSYTAHHIVGDLIGLLDHLEIDQVFLVGHDLGATIAWYFSIFRPDRVKA
Sbjct: 61  GYGDSDVPPSPSSYTAHHIVGDLIGLLDHLEIDQVFLVGHDLGATIAWYFSIFRPDRVKA 120

Query: 121 LVNLSVYHTPRIPEMPPLAIIRLFLGDDFYICKFQEPGVAEADLGSVDTATMMKKFLTLR 180
           LVNLSVYHTPRIPEMPPLAIIRLFLGDDFYICKFQEPGVAEADLGSVDTATMMKKFLTLR
Sbjct: 121 LVNLSVYHTPRIPEMPPLAIIRLFLGDDFYICKFQEPGVAEADLGSVDTATMMKKFLTLR 180

Query: 181 DPSAPIAPNGFSTLLATPETLPSWLTEEDVDYYASKFAKTGFTGGLNYYRALDLTWELTG 240
           DPSAPIAPNGFSTLLATPETLPSWLTE+DVDYYASKFAKTGFTGGLNYYRALDLTWELTG
Sbjct: 181 DPSAPIAPNGFSTLLATPETLPSWLTEDDVDYYASKFAKTGFTGGLNYYRALDLTWELTG 240

Query: 241 PWTGAKIKVPTKFIVGDQDLVYHFPGAKEYIHGDSFKEDVPLLEEVVVIEGAAHFINQEK 300
           PWTGAKIKVPTKFIVGDQDLVYHFPGAKEYIHGDSFKEDVP LEEVVVIEGAAHFINQEK
Sbjct: 241 PWTGAKIKVPTKFIVGDQDLVYHFPGAKEYIHGDSFKEDVPHLEEVVVIEGAAHFINQEK 300

Query: 301 ADEINSLIYDFITKF 316
           ADEINSLIYDFITKF
Sbjct: 301 ADEINSLIYDFITKF 315

[Close]

BLAST of CSPI04G00950 vs. NCBI nr
Match: XP_008454327.1 (PREDICTED: bifunctional epoxide hydrolase 2-like [Cucumis melo] >KAA0044386.1 bifunctional epoxide hydrolase 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 565.1 bits (1455), Expect = 3.9e-157
Identity = 263/315 (83.49%), Postives = 285/315 (90.48%), Query Frame = 0


Query: 1   MEKIQHSTIPTNGINIHLASIGSGPAVLFLHGFPEIWYTWRHQLLFFASKGFRAIAPDLR 60
           M+KIQHSTI TNGINIH ASIGSGP VLFLHGFPE+WY+WRHQLLF ASKGFRAIAPDLR
Sbjct: 1   MDKIQHSTISTNGINIHFASIGSGPVVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR 60

Query: 61  GYGDSDVPPSPSSYTAHHIVGDLIGLLDHLEIDQVFLVGHDLGATIAWYFSIFRPDRVKA 120
           G+GDSD PPSPSSYT HHIVGDLIGLLDHL IDQVFLVGHD GA +AWYF +FRPDRVKA
Sbjct: 61  GFGDSDAPPSPSSYTPHHIVGDLIGLLDHLGIDQVFLVGHDWGAMMAWYFCLFRPDRVKA 120

Query: 121 LVNLSVYHTPRIPEMPPLAIIRLFLGDDFYICKFQEPGVAEADLGSVDTATMMKKFLTLR 180
           LVNLSV++TPR P   PLA+ R +LGDDFYICKFQEPGVAEAD GSVDTATMMKKFLT+R
Sbjct: 121 LVNLSVHYTPRNPAGSPLAVTRRYLGDDFYICKFQEPGVAEADFGSVDTATMMKKFLTMR 180

Query: 181 DPSAPIAPNGFSTLLATPETLPSWLTEEDVDYYASKFAKTGFTGGLNYYRALDLTWELTG 240
           DP   I PNGF TLL TPE LPSWLTEED++Y+ASKF+KTGFTGG NYYRALD+TWELTG
Sbjct: 181 DPRPAIIPNGFKTLLETPEILPSWLTEEDIEYFASKFSKTGFTGGFNYYRALDITWELTG 240

Query: 241 PWTGAKIKVPTKFIVGDQDLVYHFPGAKEYIHGDSFKEDVPLLEEVVVIEGAAHFINQEK 300
           PW+ A+IKVPTKFIVGD DLVY+FPGAKEYIHG  FK+DVPLLE+VVVIEGAAHFINQEK
Sbjct: 241 PWSRAQIKVPTKFIVGDLDLVYNFPGAKEYIHGGGFKKDVPLLEDVVVIEGAAHFINQEK 300

Query: 301 ADEINSLIYDFITKF 316
            DEI+SLIYDFITKF
Sbjct: 301 PDEISSLIYDFITKF 315

[Close]

BLAST of CSPI04G00950 vs. NCBI nr
Match: XP_038905064.1 (epoxide hydrolase A-like [Benincasa hispida])

HSP 1 Score: 562.8 bits (1449), Expect = 1.9e-156
Identity = 265/315 (84.13%), Postives = 285/315 (90.48%), Query Frame = 0


Query: 1   MEKIQHSTIPTNGINIHLASIGSGPAVLFLHGFPEIWYTWRHQLLFFASKGFRAIAPDLR 60
           M KIQHSTIPTNGINIH+ASIGSGPAVLFLHGFPE+WY+WRHQLLF ASKGFRAIAPDLR
Sbjct: 1   MNKIQHSTIPTNGINIHVASIGSGPAVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR 60

Query: 61  GYGDSDVPPSPSSYTAHHIVGDLIGLLDHLEIDQVFLVGHDLGATIAWYFSIFRPDRVKA 120
           G+GDSDV PSPSSYT HHIVGDLIGLLDHL IDQVFLVGHD GA +AWYF +FRPDRVKA
Sbjct: 61  GFGDSDVLPSPSSYTPHHIVGDLIGLLDHLGIDQVFLVGHDWGAMMAWYFCLFRPDRVKA 120

Query: 121 LVNLSVYHTPRIPEMPPLAIIRLFLGDDFYICKFQEPGVAEADLGSVDTATMMKKFLTLR 180
           LVNLSV++TPR P + PLA +RL LGD+FY CKFQEPGVAEAD GSVDTATMMKKFLT+R
Sbjct: 121 LVNLSVHYTPRNPSISPLAGVRLLLGDNFYFCKFQEPGVAEADFGSVDTATMMKKFLTMR 180

Query: 181 DPSAPIAPNGFSTLLATPETLPSWLTEEDVDYYASKFAKTGFTGGLNYYRALDLTWELTG 240
           DPS PI PNGF T LATPETLP WLTEED+DY+ASKF KTGFTGG NYYRA DL+WELTG
Sbjct: 181 DPSPPIIPNGFKT-LATPETLPPWLTEEDIDYFASKFGKTGFTGGFNYYRATDLSWELTG 240

Query: 241 PWTGAKIKVPTKFIVGDQDLVYHFPGAKEYIHGDSFKEDVPLLEEVVVIEGAAHFINQEK 300
           PWTGA+IKVPTKFIVGD D+VYH PGAKEYIHG  FK+ VPLLEEVVVIEGAAHFINQEK
Sbjct: 241 PWTGAQIKVPTKFIVGDLDMVYHLPGAKEYIHGGGFKKKVPLLEEVVVIEGAAHFINQEK 300

Query: 301 ADEINSLIYDFITKF 316
           A +I+SLIYDFI KF
Sbjct: 301 AYDISSLIYDFIKKF 314

[Close]

BLAST of CSPI04G00950 vs. NCBI nr
Match: TYK29514.1 (bifunctional epoxide hydrolase 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 552.4 bits (1422), Expect = 2.6e-153
Identity = 263/337 (78.04%), Postives = 285/337 (84.57%), Query Frame = 0


Query: 1   MEKIQHSTIPTNGINIHLASIGSGPAVLFLHGFPEIWYTWRHQLLFFASKGFRAIAPDLR 60
           M+KIQHSTI TNGINIH ASIGSGP VLFLHGFPE+WY+WRHQLLF ASKGFRAIAPDLR
Sbjct: 1   MDKIQHSTISTNGINIHFASIGSGPVVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR 60

Query: 61  GYGDSDVPPSPSSYTAHHIVGDLIGLLDHLEIDQVFLVGHDLGATIAWYFSIFRPDRVKA 120
           G+GDSD PPSPSSYT HHIVGDLIGLLDHL IDQVFLVGHD GA +AWYF +FRPDRVKA
Sbjct: 61  GFGDSDAPPSPSSYTPHHIVGDLIGLLDHLGIDQVFLVGHDWGAMMAWYFCLFRPDRVKA 120

Query: 121 LVNLSVYHTPRIPEMPPLAIIRLFLGDDFYICKFQ----------------------EPG 180
           LVNLSV++TPR P   PLA+ R +LGDDFYICKFQ                      EPG
Sbjct: 121 LVNLSVHYTPRNPAGSPLAVTRRYLGDDFYICKFQVNAVDSLNSSVISEIEWLRSHWEPG 180

Query: 181 VAEADLGSVDTATMMKKFLTLRDPSAPIAPNGFSTLLATPETLPSWLTEEDVDYYASKFA 240
           VAEAD GSVDTATMMKKFLT+RDP   I PNGF TLL TPE LPSWLTEED++Y+ASKF+
Sbjct: 181 VAEADFGSVDTATMMKKFLTMRDPRPAIIPNGFKTLLETPEILPSWLTEEDIEYFASKFS 240

Query: 241 KTGFTGGLNYYRALDLTWELTGPWTGAKIKVPTKFIVGDQDLVYHFPGAKEYIHGDSFKE 300
           KTGFTGG NYYRALD+TWELTGPW+ A+IKVPTKFIVGD DLVY+FPGAKEYIHG  FK+
Sbjct: 241 KTGFTGGFNYYRALDITWELTGPWSRAQIKVPTKFIVGDLDLVYNFPGAKEYIHGGGFKK 300

Query: 301 DVPLLEEVVVIEGAAHFINQEKADEINSLIYDFITKF 316
           DVPLLE+VVVIEGAAHFINQEK DEI+SLIYDFITKF
Sbjct: 301 DVPLLEDVVVIEGAAHFINQEKPDEISSLIYDFITKF 337

[Close]

BLAST of CSPI04G00950 vs. NCBI nr
Match: XP_022934351.1 (uncharacterized protein LOC111441542 [Cucurbita moschata])

HSP 1 Score: 550.8 bits (1418), Expect = 7.5e-153
Identity = 255/315 (80.95%), Postives = 283/315 (89.84%), Query Frame = 0


Query: 1   MEKIQHSTIPTNGINIHLASIGSGPAVLFLHGFPEIWYTWRHQLLFFASKGFRAIAPDLR 60
           MEKI+HSTI TNGIN+H+ASIGSGP VLFLHGFPE+WY+WRHQLLF ASKGFRAIAPDLR
Sbjct: 1   MEKIEHSTIATNGINMHVASIGSGPPVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR 60

Query: 61  GYGDSDVPPSPSSYTAHHIVGDLIGLLDHLEIDQVFLVGHDLGATIAWYFSIFRPDRVKA 120
           G+GDSDVPPSPSSYT  HI+GDLIGLLDHL I+QVFLVGHD GA +AWYF +FRPDRVKA
Sbjct: 61  GFGDSDVPPSPSSYTPFHIIGDLIGLLDHLGIEQVFLVGHDWGAMMAWYFCLFRPDRVKA 120

Query: 121 LVNLSVYHTPRIPEMPPLAIIRLFLGDDFYICKFQEPGVAEADLGSVDTATMMKKFLTLR 180
           LVNLSV++ PR P + PL+  R FLGDDFYICKFQ PGVAEAD GSVDTATMMKKFLT+R
Sbjct: 121 LVNLSVHYNPRNPAISPLSRTRQFLGDDFYICKFQTPGVAEADFGSVDTATMMKKFLTIR 180

Query: 181 DPSAPIAPNGFSTLLATPETLPSWLTEEDVDYYASKFAKTGFTGGLNYYRALDLTWELTG 240
           DPS PI PNGF T L TPETLPSWLTEED+DY+ASKF KTGFTGG NYYRA++ TWELTG
Sbjct: 181 DPSPPIIPNGFKT-LKTPETLPSWLTEEDIDYFASKFTKTGFTGGFNYYRAIEQTWELTG 240

Query: 241 PWTGAKIKVPTKFIVGDQDLVYHFPGAKEYIHGDSFKEDVPLLEEVVVIEGAAHFINQEK 300
           PW+GAKIKVPTK++VGD D+VYH PGAK+YIHG  FK+DVPLLEEVVV+EGAAHFINQEK
Sbjct: 241 PWSGAKIKVPTKYVVGDVDMVYHLPGAKQYIHGGGFKKDVPLLEEVVVMEGAAHFINQEK 300

Query: 301 ADEINSLIYDFITKF 316
           ADEI++ IYDFI KF
Sbjct: 301 ADEISAHIYDFIIKF 314

[Close]

BLAST of CSPI04G00950 vs. TAIR 10
Match: AT4G02340.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 426.4 bits (1095), Expect = 2.0e-119
Identity = 194/317 (61.20%), Postives = 237/317 (74.76%), Query Frame = 0


Query: 1   MEKIQHSTIPTNGINIHLASIGSGPAVLFLHGFPEIWYTWRHQLLFFASKGFRAIAPDLR 60
           MEKI+H+TI TNGIN+H+ASIGSGP +LF+HGFP++WY+WRHQL+ FA+ G+RAIAPDLR
Sbjct: 1   MEKIEHTTISTNGINMHVASIGSGPVILFVHGFPDLWYSWRHQLVSFAALGYRAIAPDLR 60

Query: 61  GYGDSDVPPSPSSYTAHHIVGDLIGLLDHLEIDQVFLVGHDLGATIAWYFSIFRPDRVKA 120
           GYGDSD PPS  SYT  HIVGDL+GLLD L +D+VFLVGHD GA +AW+  + RPDRV A
Sbjct: 61  GYGDSDAPPSRESYTILHIVGDLVGLLDSLGVDRVFLVGHDWGAIVAWWLCMIRPDRVNA 120

Query: 121 LVNLSVYHTPRIPEMPPLAIIRLFLGDDFYICKFQEPGVAEADLGSVDTATMMKKFLTLR 180
           LVN SV   PR P + P+   R   GDD+YIC+FQEPG  E D   VDT  ++ +F T R
Sbjct: 121 LVNTSVVFNPRNPSVKPVDAFRALFGDDYYICRFQEPGEIEEDFAQVDTKKLITRFFTSR 180

Query: 181 DPSAPIAPN--GFSTLLATPETLPSWLTEEDVDYYASKFAKTGFTGGLNYYRALDLTWEL 240
           +P  P  P   GF   L  P +LP+WLTE+DV +Y  KF++ GFTGGLNYYRAL+L+WEL
Sbjct: 181 NPRPPCIPKSVGFRG-LPDPPSLPAWLTEQDVRFYGDKFSQKGFTGGLNYYRALNLSWEL 240

Query: 241 TGPWTGAKIKVPTKFIVGDQDLVYHFPGAKEYIHGDSFKEDVPLLEEVVVIEGAAHFINQ 300
           T PWTG +IKVP KFIVGD D+ Y+ PG KEYIH    K+ VP L+EVVV+EG  HF++Q
Sbjct: 241 TAPWTGLQIKVPVKFIVGDLDITYNIPGTKEYIHEGGLKKHVPFLQEVVVMEGVGHFLHQ 300

Query: 301 EKADEINSLIYDFITKF 316
           EK DE+   IY F  KF
Sbjct: 301 EKPDEVTDHIYGFFKKF 316

[Close]

BLAST of CSPI04G00950 vs. TAIR 10
Match: AT2G26740.1 (soluble epoxide hydrolase )

HSP 1 Score: 374.0 bits (959), Expect = 1.2e-103
Identity = 185/321 (57.63%), Postives = 229/321 (71.34%), Query Frame = 0


Query: 4   IQHSTIPTNGINIHLASIG--SGPAVLFLHGFPEIWYTWRHQLLFFASKGFRAIAPDLRG 63
           ++H  +  NGI+IH+A  G   GP VL LHGFPE+WY+WRHQ+   A++G+RA+APDLRG
Sbjct: 1   MEHRKVRGNGIDIHVAIQGPSDGPIVLLLHGFPELWYSWRHQIPGLAARGYRAVAPDLRG 60

Query: 64  YGDSDVPPSPSSYTAHHIVGDLIGLLDHL---EIDQVFLVGHDLGATIAWYFSIFRPDRV 123
           YGDSD P   SSYT  +IVGDLI ++  L   E ++VF+VGHD GA IAWY  +FRPDRV
Sbjct: 61  YGDSDAPAEISSYTCFNIVGDLIAVISALTASEDEKVFVVGHDWGALIAWYLCLFRPDRV 120

Query: 124 KALVNLSVYHT--PRIPEMPPLAIIRLFLGDDFYICKFQEPGVAEADLGSVDTATMMKKF 183
           KALVNLSV  +  P  P + P+  +R F GDD+YIC+FQE G  EA++  V T  +MK+ 
Sbjct: 121 KALVNLSVPFSFRPTDPSVKPVDRMRAFYGDDYYICRFQEFGDVEAEIAEVGTERVMKRL 180

Query: 184 LTLRDPSAPIAPNGFSTLLATPET--LPSWLTEEDVDYYASKFAKTGFTGGLNYYRALDL 243
           LT R P   I P   S   +  ET  LPSWLTEEDV Y+ SKF + GF+G +NYYR  + 
Sbjct: 181 LTYRTPGPVIIPKDKSFWGSKGETIPLPSWLTEEDVAYFVSKFEEKGFSGPVNYYRNFNR 240

Query: 244 TWELTGPWTGAKIKVPTKFIVGDQDLVYHFPGAKEYIHGDSFKEDVPLLEEVVVIEGAAH 303
             EL GPW G+KI+VPTKF++G+ DLVY+ PG KEYIHG  FKEDVPLLEE VV+EG AH
Sbjct: 241 NNELLGPWVGSKIQVPTKFVIGELDLVYYMPGVKEYIHGPQFKEDVPLLEEPVVMEGVAH 300

Query: 304 FINQEKADEINSLIYDFITKF 316
           FINQEK  EI  +I DFI+KF
Sbjct: 301 FINQEKPQEILQIILDFISKF 321

[Close]

BLAST of CSPI04G00950 vs. TAIR 10
Match: AT3G05600.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 357.8 bits (917), Expect = 8.7e-99
Identity = 171/324 (52.78%), Postives = 216/324 (66.67%), Query Frame = 0


Query: 1   MEKIQHSTIPTNGINIHLASIG--SGPAVLFLHGFPEIWYTWRHQLLFFASKGFRAIAPD 60
           ME I H  +  NGI +H+A  G   GP VL LHGFP++WYTWRHQ+   +S G+RA+APD
Sbjct: 1   MEGIDHRMVSVNGITMHIAEKGPKEGPVVLLLHGFPDLWYTWRHQISGLSSLGYRAVAPD 60

Query: 61  LRGYGDSDVPPSPSSYTAHHIVGDLIGLLDHLEIDQ--VFLVGHDLGATIAWYFSIFRPD 120
           LRGYGDSD P S S YT  ++VGDL+ LLD +  +Q  VFLVGHD GA I W+  +FRP+
Sbjct: 61  LRGYGDSDSPESFSEYTCLNVVGDLVALLDSVAGNQEKVFLVGHDWGAIIGWFLCLFRPE 120

Query: 121 RVKALVNLSVYHTPRIPEMPPLAIIRLFLGDDFYICKFQEPGVAEADLGSVDTATMMKKF 180
           ++   V LSV +  R P++ P+   +   GDD+YIC+FQEPG  E ++ S D    ++  
Sbjct: 121 KINGFVCLSVPYRSRNPKVKPVQGFKAVFGDDYYICRFQEPGKIEGEIASADPRIFLRNL 180

Query: 181 LTLRDPSAPIAP--NGFSTLLATPET----LPSWLTEEDVDYYASKFAKTGFTGGLNYYR 240
            T R    PI P  N F      P +    LP W +++D+D+Y SKF K GFTGGLNYYR
Sbjct: 181 FTGRTLGPPILPKDNPFGE-KPNPNSENIELPEWFSKKDLDFYVSKFEKAGFTGGLNYYR 240

Query: 241 ALDLTWELTGPWTGAKIKVPTKFIVGDQDLVYHFPGAKEYIHGDSFKEDVPLLEEVVVIE 300
           A+DL WELT PWTGAKI+VP KF+ GD D+VY  PG KEYIHG  F  DVP L+E+VVIE
Sbjct: 241 AMDLNWELTAPWTGAKIQVPVKFMTGDFDMVYTTPGMKEYIHGGGFAADVPTLQEIVVIE 300

Query: 301 GAAHFINQEKADEINSLIYDFITK 315
            A HF+NQEK  E+ + I DF TK
Sbjct: 301 DAGHFVNQEKPQEVTAHINDFFTK 323

[Close]

BLAST of CSPI04G00950 vs. TAIR 10
Match: AT2G26750.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 357.5 bits (916), Expect = 1.1e-98
Identity = 175/320 (54.69%), Postives = 223/320 (69.69%), Query Frame = 0


Query: 4   IQHSTIPTNGINIHLASIG--SGPAVLFLHGFPEIWYTWRHQLLFFASKGFRAIAPDLRG 63
           ++H  +  NGI+IH+A  G   G  VL LHGFPE+WY+WRHQ+   A++G+RA+APDLRG
Sbjct: 1   MEHRNVRGNGIDIHVAIQGPSDGTIVLLLHGFPELWYSWRHQISGLAARGYRAVAPDLRG 60

Query: 64  YGDSDVPPSPSSYTAHHIVGDLIGLLDHL--EIDQVFLVGHDLGATIAWYFSIFRPDRVK 123
           YGDSD P   SS+T  +IVGDL+ ++  L  E  +VF+VGHD GA IAWY  +FRPD+VK
Sbjct: 61  YGDSDAPAEISSFTCFNIVGDLVAVISTLIKEDKKVFVVGHDWGALIAWYLCLFRPDKVK 120

Query: 124 ALVNLSV--YHTPRIPEMPPLAIIRLFLGDDFYICKFQEPGVAEADLGSVDTATMMKKFL 183
           ALVNLSV     P  P + P+  +R   G+D+Y+C+FQE G  EA++  V T  +MK+ L
Sbjct: 121 ALVNLSVPLSFWPTDPSVKPVDRMRAVYGNDYYVCRFQEVGDIEAEIAEVGTERVMKRLL 180

Query: 184 TLRDPSAPIAPNGFSTLLATPET--LPSWLTEEDVDYYASKFAKTGFTGGLNYYRALDLT 243
           T R P   I P   S   +  ET  LPSWLTEEDV Y+ SKF + GF G +NYYR  +  
Sbjct: 181 TYRTPGPLIIPKDKSFWGSKGETIPLPSWLTEEDVAYFVSKFKEKGFCGPVNYYRNFNRN 240

Query: 244 WELTGPWTGAKIKVPTKFIVGDQDLVYHFPGAKEYIHGDSFKEDVPLLEEVVVIEGAAHF 303
            EL GPW G+KI+VPTKF++G+ DLVY+ PG KEYIHG  FKEDVPL+EE VV+EG AHF
Sbjct: 241 NELLGPWVGSKIQVPTKFVIGELDLVYYMPGVKEYIHGPQFKEDVPLIEEPVVMEGVAHF 300

Query: 304 INQEKADEINSLIYDFITKF 316
           +NQEK  EI  +I DFI+ F
Sbjct: 301 LNQEKPQEILQIILDFISTF 320

[Close]

BLAST of CSPI04G00950 vs. TAIR 10
Match: AT4G15960.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 349.0 bits (894), Expect = 4.1e-96
Identity = 159/324 (49.07%), Postives = 216/324 (66.67%), Query Frame = 0


Query: 1   MEKIQHSTIPTNGINIHLA-----SIGSGPAVLFLHGFPEIWYTWRHQLLFFASKGFRAI 60
           ++ ++H T+  NGIN+H+A       G  P +LFLHGFPE+WYTWRHQ++  +S G+R I
Sbjct: 51  LDGVEHKTLKVNGINMHVAEKPGSGSGEDPIILFLHGFPELWYTWRHQMVALSSLGYRTI 110

Query: 61  APDLRGYGDSDVPPSPSSYTAHHIVGDLIGLLDHLE--IDQVFLVGHDLGATIAWYFSIF 120
           APDLRGYGD++ P     YT  ++ GD++ L+D +      V +VGHD GA IAW    +
Sbjct: 111 APDLRGYGDTEAPEKVEDYTYLNVDGDVVALIDAVTGGDKAVSVVGHDWGAMIAWQLCQY 170

Query: 121 RPDRVKALVNLSVYHTPRIPEMPPLAIIRLFLGDDFYICKFQEPGVAEADLGSVDTATMM 180
           RP++VKALVN+SV  +PR P   P+  +R   GDD+Y+C+FQ+ G  E +   + T  ++
Sbjct: 171 RPEKVKALVNMSVLFSPRNPVRVPVPTLRHVFGDDYYVCRFQKAGEIETEFKKLGTENVL 230

Query: 181 KKFLTLRDPSAPIAPNG--FSTLLATPETLPSWLTEEDVDYYASKFAKTGFTGGLNYYRA 240
           K+FLT + P     P    F         LP WLT+ED+DYY +K+   GFTG +NYYR 
Sbjct: 231 KEFLTYKTPGPLNLPKDKYFKRSENAASALPLWLTQEDLDYYVTKYENKGFTGPINYYRN 290

Query: 241 LDLTWELTGPWTGAKIKVPTKFIVGDQDLVYHFPGAKEYIHGDSFKEDVPLLEEVVVIEG 300
           +D  WELT PWTGAKI+VP KFI+GDQDL Y+FPGAKEYI+G  FK DVPLL+E VV++G
Sbjct: 291 IDRNWELTAPWTGAKIRVPVKFIIGDQDLTYNFPGAKEYINGGGFKRDVPLLDETVVLKG 350

Query: 301 AAHFINQEKADEINSLIYDFITKF 316
             HF+++E  D IN  I++F  KF
Sbjct: 351 LGHFLHEENPDVINQHIHNFFHKF 374

The following BLAST results are available for this feature:

BLAST of CSPI04G00950 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
I6YGS0	4.6e-60	38.87	Epoxide hydrolase A OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX...	[more]
P34914	8.9e-48	33.66	Bifunctional epoxide hydrolase 2 OS=Mus musculus OX=10090 GN=Ephx2 PE=1 SV=2	[more]
Q6Q2C2	1.2e-47	33.33	Bifunctional epoxide hydrolase 2 OS=Sus scrofa OX=9823 GN=EPHX2 PE=2 SV=1	[more]
P80299	4.4e-47	33.01	Bifunctional epoxide hydrolase 2 OS=Rattus norvegicus OX=10116 GN=Ephx2 PE=1 SV=...	[more]
P34913	6.4e-46	32.13	Bifunctional epoxide hydrolase 2 OS=Homo sapiens OX=9606 GN=EPHX2 PE=1 SV=2	[more]

BLAST of CSPI04G00950 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KWK9	2.3e-184	98.73	Epoxide hydrolase 2 OS=Cucumis sativus OX=3659 GN=Csa_4G001890 PE=4 SV=1	[more]
A0A5A7TQJ9	1.9e-157	83.49	Bifunctional epoxide hydrolase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=...	[more]
A0A1S3BZ44	1.9e-157	83.49	bifunctional epoxide hydrolase 2-like OS=Cucumis melo OX=3656 GN=LOC103494761 PE...	[more]
A0A5D3E108	1.3e-153	78.04	Bifunctional epoxide hydrolase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=...	[more]
A0A6J1F1K3	3.7e-153	80.95	uncharacterized protein LOC111441542 OS=Cucurbita moschata OX=3662 GN=LOC1114415...	[more]

BLAST of CSPI04G00950 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004152244.1	4.8e-184	98.73	uncharacterized protein LOC101204815 [Cucumis sativus] >KGN52822.1 hypothetical ...	[more]
XP_008454327.1	3.9e-157	83.49	PREDICTED: bifunctional epoxide hydrolase 2-like [Cucumis melo] >KAA0044386.1 bi...	[more]
XP_038905064.1	1.9e-156	84.13	epoxide hydrolase A-like [Benincasa hispida]	[more]
TYK29514.1	2.6e-153	78.04	bifunctional epoxide hydrolase 2-like [Cucumis melo var. makuwa]	[more]
XP_022934351.1	7.5e-153	80.95	uncharacterized protein LOC111441542 [Cucurbita moschata]	[more]

BLAST of CSPI04G00950 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G02340.1	2.0e-119	61.20	alpha/beta-Hydrolases superfamily protein	[more]
AT2G26740.1	1.2e-103	57.63	soluble epoxide hydrolase	[more]
AT3G05600.1	8.7e-99	52.78	alpha/beta-Hydrolases superfamily protein	[more]
AT2G26750.1	1.1e-98	54.69	alpha/beta-Hydrolases superfamily protein	[more]
AT4G15960.1	4.1e-96	49.07	alpha/beta-Hydrolases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000639	Epoxide hydrolase-like	PRINTS	PR00412	EPOXHYDRLASE	coord: 289..311 score: 39.33 coord: 51..66 score: 62.5 coord: 111..124 score: 49.68 coord: 97..110 score: 44.81 coord: 31..49 score: 46.89 coord: 249..265 score: 43.05
IPR000073	Alpha/beta hydrolase fold-1	PRINTS	PR00111	ABHYDROLASE	coord: 97..110 score: 32.51 coord: 111..124 score: 32.02 coord: 51..66 score: 46.55
IPR000073	Alpha/beta hydrolase fold-1	PFAM	PF00561	Abhydrolase_1	coord: 25..138 e-value: 4.6E-23 score: 82.2
IPR029058	Alpha/Beta hydrolase fold	GENE3D	3.40.50.1820	alpha/beta hydrolase	coord: 1..315 e-value: 7.0E-107 score: 359.8
IPR029058	Alpha/Beta hydrolase fold	SUPERFAMILY	53474	alpha/beta-Hydrolases	coord: 2..314
None	No IPR available	PANTHER	PTHR43329:SF58	OS05G0273800 PROTEIN	coord: 1..315
None	No IPR available	PANTHER	PTHR43329	EPOXIDE HYDROLASE	coord: 1..315

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI04G00950.1	CSPI04G00950.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016787	hydrolase activity
molecular_function	GO:0003824	catalytic activity