CSPI04G00590 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI04G00590
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Location	Chr4: 302176 .. 303915 (+)
RNA-Seq Expression	CSPI04G00590
Synteny	CSPI04G00590

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CCCACCAAACACTTAGAAAAAAGAAAAAGGAATTAGAAGGAAAAATAAAAGAGTGTAAAAGGGAATGGATAGTGAATCCTCTCTTAAGCTTCCCCTAATTGATTTCTCCAATTTAGAATCAGGGGGTCCTAAGTGGGAGTTGGCAAAAGCACAAGTGAAGGAAGCACTTGAGGAATTTGGTTGCTTTGAAGCCTCATTTGACAAAGTGCCTGTTGAGGTCAGAAAGGGTTTGTTTGAAGCACTTGAAGAGCTTTTCAACCTTCCTTTGGAAACCAAATTGAGAAATGTTTCTCAAAAGCCCTTCCATGGCTATGTTGGACAGTACCCAATGGCTCCACTTTTTGAAAGTATGGGTGTGGATGATTCCACCATACCTCAAAAGGTCCAAGACTTCACCAATATCTTGTGGCCTCAAGGAAACCCAACCTTTAGGTTTGAATATGTTCTACAAATAATGCTATCTTATTAATTATTTCCCTCTTCTCCTTCTCATTTTTATTTCATACTTTGAATCTATATTATACGTTATTACTTGTTTATTTTTTCTTACCTTTATCGATCGAAAATATGTCTTAACTAGTTGTGATCTTAATTTTGATGACTAATTAATGATAAGAGTCTCTGTCTTGAACCTTCTAATTAGGGTTTTTTTTCCTTTTCTTACAAATCTTGTTGAGTACAGCAAAGTGATGGAAACCTATTCACAACAATTAGCAGAACTAGACGAGATGGTGAGAAGAATGGTTTTGGAGAGTTTGGGAGTTGAAAAGTATTTGGAGGAACATTTGGAGTCAACAAACTATCTTCTTAGAGTGATGAAATACAAAGGGACTGAAAGCAATTATGAAACAAAAATTGGGCTTCATTCCCACACTGATAAGAACATTGTAACCATTTTATATCAAAACCATGTTCAAGGTTTACAGGTAAAAACCAAAGATGGGAAATGGATTAACTTTCAACCTTCCCCAGACTCCTTTGTTGCAATGATTGGAGATTCCTTCCATGTGAGTAACCTTATATTACTTCATTTTTGTTTTCTGATATATATTCATTTTATTAAAAAAAAAATGGTATACTTCAGAAAAAACTTTGTATTATTTATTACTATTTTTTTGTTTTCGAAGTTTGGTTGGGGATTTTTAATTTAATTTAATTATTGTTATTATCTAAACATAACTTTTATGCATGGTAATTAATTAATTGCTTTAGTCCTCCTCAACTTTGTAGTTTTGGTAAAAGGTGTTCCTAACTTTCGAATATCTCAAAAATTTGGAAAAATTTAATAAACACCCTTTACACTTAGAAAAGTTAAAAGATGATATATGATATAGATATATGCATGTAGGCATGGACAAATGGGAGATTGCATTCTCCATATCATAGAGTGATGATGAGTGGAGAAGAAGTGAGGTACTCAGCTGGGTTATTCTCAATCCCAAAGGCAGGCTACATAGTGAAAGCACCAGAAGAGCTTGTAGATGAACAACATCCTTTGCTCTTCAATCCATTTGATCATGTTCAATTTCTTCAGTTCTACTATACAGAAGCTGGCCAAAAAGCTCAATCTGCCCTCAAAACTTATTGTGGTGCAACTTAGAAACATTGATATATATATATATATCTTTCTTGCTTCAACAATAATATTTTCTTTTTTGTATTTGGACTACATATATAATATTATTACGTCTTTGATGCTTGTGTATCATTTTCTTTAGTTTAATAATATCCATCCAAGAC

mRNA sequence

CCCACCAAACACTTAGAAAAAAGAAAAAGGAATTAGAAGGAAAAATAAAAGAGTGTAAAAGGGAATGGATAGTGAATCCTCTCTTAAGCTTCCCCTAATTGATTTCTCCAATTTAGAATCAGGGGGTCCTAAGTGGGAGTTGGCAAAAGCACAAGTGAAGGAAGCACTTGAGGAATTTGGTTGCTTTGAAGCCTCATTTGACAAAGTGCCTGTTGAGGTCAGAAAGGGTTTGTTTGAAGCACTTGAAGAGCTTTTCAACCTTCCTTTGGAAACCAAATTGAGAAATGTTTCTCAAAAGCCCTTCCATGGCTATGTTGGACAGTACCCAATGGCTCCACTTTTTGAAAGTATGGGTGTGGATGATTCCACCATACCTCAAAAGGTCCAAGACTTCACCAATATCTTGTGGCCTCAAGGAAACCCAACCTTTAGCAAAGTGATGGAAACCTATTCACAACAATTAGCAGAACTAGACGAGATGGTGAGAAGAATGGTTTTGGAGAGTTTGGGAGTTGAAAAGTATTTGGAGGAACATTTGGAGTCAACAAACTATCTTCTTAGAGTGATGAAATACAAAGGGACTGAAAGCAATTATGAAACAAAAATTGGGCTTCATTCCCACACTGATAAGAACATTGTAACCATTTTATATCAAAACCATGTTCAAGGTTTACAGGTAAAAACCAAAGATGGGAAATGGATTAACTTTCAACCTTCCCCAGACTCCTTTGTTGCAATGATTGGAGATTCCTTCCATGCATGGACAAATGGGAGATTGCATTCTCCATATCATAGAGTGATGATGAGTGGAGAAGAAGTGAGGTACTCAGCTGGGTTATTCTCAATCCCAAAGGCAGGCTACATAGTGAAAGCACCAGAAGAGCTTGTAGATGAACAACATCCTTTGCTCTTCAATCCATTTGATCATGTTCAATTTCTTCAGTTCTACTATACAGAAGCTGGCCAAAAAGCTCAATCTGCCCTCAAAACTTATTGTGGTGCAACTTAGAAACATTGATATATATATATATATCTTTCTTGCTTCAACAATAATATTTTCTTTTTTGTATTTGGACTACATATATAATATTATTACGTCTTTGATGCTTGTGTATCATTTTCTTTAGTTTAATAATATCCATCCAAGAC

Coding sequence (CDS)

ATGGATAGTGAATCCTCTCTTAAGCTTCCCCTAATTGATTTCTCCAATTTAGAATCAGGGGGTCCTAAGTGGGAGTTGGCAAAAGCACAAGTGAAGGAAGCACTTGAGGAATTTGGTTGCTTTGAAGCCTCATTTGACAAAGTGCCTGTTGAGGTCAGAAAGGGTTTGTTTGAAGCACTTGAAGAGCTTTTCAACCTTCCTTTGGAAACCAAATTGAGAAATGTTTCTCAAAAGCCCTTCCATGGCTATGTTGGACAGTACCCAATGGCTCCACTTTTTGAAAGTATGGGTGTGGATGATTCCACCATACCTCAAAAGGTCCAAGACTTCACCAATATCTTGTGGCCTCAAGGAAACCCAACCTTTAGCAAAGTGATGGAAACCTATTCACAACAATTAGCAGAACTAGACGAGATGGTGAGAAGAATGGTTTTGGAGAGTTTGGGAGTTGAAAAGTATTTGGAGGAACATTTGGAGTCAACAAACTATCTTCTTAGAGTGATGAAATACAAAGGGACTGAAAGCAATTATGAAACAAAAATTGGGCTTCATTCCCACACTGATAAGAACATTGTAACCATTTTATATCAAAACCATGTTCAAGGTTTACAGGTAAAAACCAAAGATGGGAAATGGATTAACTTTCAACCTTCCCCAGACTCCTTTGTTGCAATGATTGGAGATTCCTTCCATGCATGGACAAATGGGAGATTGCATTCTCCATATCATAGAGTGATGATGAGTGGAGAAGAAGTGAGGTACTCAGCTGGGTTATTCTCAATCCCAAAGGCAGGCTACATAGTGAAAGCACCAGAAGAGCTTGTAGATGAACAACATCCTTTGCTCTTCAATCCATTTGATCATGTTCAATTTCTTCAGTTCTACTATACAGAAGCTGGCCAAAAAGCTCAATCTGCCCTCAAAACTTATTGTGGTGCAACTTAG

Protein sequence

MDSESSLKLPLIDFSNLESGGPKWELAKAQVKEALEEFGCFEASFDKVPVEVRKGLFEALEELFNLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTIPQKVQDFTNILWPQGNPTFSKVMETYSQQLAELDEMVRRMVLESLGVEKYLEEHLESTNYLLRVMKYKGTESNYETKIGLHSHTDKNIVTILYQNHVQGLQVKTKDGKWINFQPSPDSFVAMIGDSFHAWTNGRLHSPYHRVMMSGEEVRYSAGLFSIPKAGYIVKAPEELVDEQHPLLFNPFDHVQFLQFYYTEAGQKAQSALKTYCGAT*

Homology

[Close]

BLAST of CSPI04G00590 vs. ExPASy Swiss-Prot
Match: Q945B6 (Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana OX=3702 GN=AOP1.2 PE=2 SV=1)

HSP 1 Score: 364.0 bits (933), Expect = 1.7e-99
Identity = 176/312 (56.41%), Postives = 231/312 (74.04%), Query Frame = 0


Query: 6   SLKLPLIDFS--NLESGGPKWELAKAQVKEALEEFGCFEASFDKVPVEVRKGLFEALEEL 65
           S +LP+IDFS  NL+ G  KW+  KA V +ALE++GCFEA FDK+ VE+ + +FEA+E+L
Sbjct: 11  SFQLPVIDFSDQNLKPGSSKWDEVKADVLKALEDYGCFEAFFDKLSVELNRSVFEAMEDL 70

Query: 66  FNLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTIPQKVQDFTNILWP-QGNPTF 125
           F LP+ TK RNVS KPFHGY+       L+ES+G+DD+ + +KV DFT  LWP  GN + 
Sbjct: 71  FELPIPTKQRNVSSKPFHGYL----CHNLYESLGIDDANVLEKVNDFTQQLWPDHGNKSI 130

Query: 126 SKVMETYSQQLAELDEMVRRMVLESLGVEKYLEEHLESTNYLLRVMKYKG----TESNYE 185
           S+ +  +S+QL ELD MVRRM++ES G+E Y++EHL ST YL R+MKY       + + E
Sbjct: 131 SETIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYTSPPDDDDDDEE 190

Query: 186 TKIGLHSHTDKNIVTILYQNHVQGLQVKTKDGKWINFQPSPDSFVAMIGDSFHAWTNGRL 245
           TK+GL SHTDKNI+TIL+Q  V GL+VKTKD KWI  +PS DS + M+GDS  A  NGRL
Sbjct: 191 TKLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKPSQDSVLVMVGDSLCALLNGRL 250

Query: 246 HSPYHRVMMSGEEVRYSAGLFSIPKAGYIVKAPEELVDEQHPLLFNPFDHVQFLQFYYTE 305
           HSPYHRV+M+G++ RYS GLFSIPK G I+ +PEELVD++HP +F PF++  FL F+ TE
Sbjct: 251 HSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHFFQTE 310

Query: 306 AGQKAQSALKTY 311
           AG+ AQSAL  +
Sbjct: 311 AGRIAQSALHAF 318

[Close]

BLAST of CSPI04G00590 vs. ExPASy Swiss-Prot
Match: Q9ZTA3 (Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)

HSP 1 Score: 361.7 bits (927), Expect = 8.5e-99
Identity = 175/313 (55.91%), Postives = 231/313 (73.80%), Query Frame = 0


Query: 6   SLKLPLIDFS--NLESGGPKWELAKAQVKEALEEFGCFEASFDKVPVEVRKGLFEALEEL 65
           S +LP+IDFS  NL+ G  KW+   A V +ALE++GCFEASFDK+ VE+ + +FEA+E+L
Sbjct: 11  SFQLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDL 70

Query: 66  FNLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTIPQKVQDFTNILWP-QGNPTF 125
           F LP+ TK RNVS KPFHGY+       L+ES+G++D+ + +KV DFT  LWP  GN + 
Sbjct: 71  FELPIPTKQRNVSSKPFHGYL----CHNLYESLGINDANVLEKVNDFTQQLWPDHGNKSI 130

Query: 126 SKVMETYSQQLAELDEMVRRMVLESLGVEKYLEEHLESTNYLLRVMKYKG-----TESNY 185
           S+ +  +S+QL ELD MVRRM++ES G+E Y++EHL ST YL R+MKY        + + 
Sbjct: 131 SETIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYTSPPDDDDDDDE 190

Query: 186 ETKIGLHSHTDKNIVTILYQNHVQGLQVKTKDGKWINFQPSPDSFVAMIGDSFHAWTNGR 245
           ETK+GL SHTDKNI+TIL+Q  V GL+VKTKD KWI  +PS DS + M+GDS  A  NGR
Sbjct: 191 ETKLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKPSQDSVLVMVGDSLCALLNGR 250

Query: 246 LHSPYHRVMMSGEEVRYSAGLFSIPKAGYIVKAPEELVDEQHPLLFNPFDHVQFLQFYYT 305
           LHSPYHRV+M+G++ RYS GLFSIPK G I+ +PEELVD++HP +F PF++  FL F+ T
Sbjct: 251 LHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHFFQT 310

Query: 306 EAGQKAQSALKTY 311
           EAG+ AQSAL  +
Sbjct: 311 EAGRIAQSALHAF 319

[Close]

BLAST of CSPI04G00590 vs. ExPASy Swiss-Prot
Match: Q944Z5 (Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)

HSP 1 Score: 352.4 bits (903), Expect = 5.1e-96
Identity = 170/302 (56.29%), Postives = 225/302 (74.50%), Query Frame = 0


Query: 6   SLKLPLIDFS--NLESGGPKWELAKAQVKEALEEFGCFEASFDKVPVEVRKGLFEALEEL 65
           S +LP+IDFS  NL+ G  KW+   A V +ALE++GCFEASFDK+ VE+ + +FEA+E+L
Sbjct: 4   SFQLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDL 63

Query: 66  FNLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTIPQKVQDFTNILWP-QGNPTF 125
           F LP+ TK RNVS K FHGY+       L+ES+G++D+ + +KV DFT  LWP  GN + 
Sbjct: 64  FELPIPTKQRNVSSKLFHGYL----CHNLYESLGINDANVLEKVNDFTQQLWPDHGNKSI 123

Query: 126 SKVMETYSQQLAELDEMVRRMVLESLGVEKYLEEHLESTNYLLRVMKYKG---TESNYET 185
           S+ +  +S+QL ELD MVRRM++ES G+EKY++EHL ST YL R+MKY      + + ET
Sbjct: 124 SETIHLFSEQLVELDLMVRRMIMESFGIEKYIDEHLNSTYYLTRLMKYTSPPDDDDDEET 183

Query: 186 KIGLHSHTDKNIVTILYQNHVQGLQVKTKDGKWINFQPSPDSFVAMIGDSFHAWTNGRLH 245
           K+GL SHTDKNI+TIL+Q  V GL+VKTKD KWI  +PS DS + M+GDS  A  NGRLH
Sbjct: 184 KLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKPSQDSVLVMVGDSLCALLNGRLH 243

Query: 246 SPYHRVMMSGEEVRYSAGLFSIPKAGYIVKAPEELVDEQHPLLFNPFDHVQFLQFYYTEA 302
           SPYHRV+M+G++ RYS GLFSIPK G I+ +PEELVD++HP +F PF++  FL F+ TEA
Sbjct: 244 SPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHFFQTEA 301

[Close]

BLAST of CSPI04G00590 vs. ExPASy Swiss-Prot
Match: Q9ZTA2 (Probable inactive 2-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thaliana OX=3702 GN=AOP2 PE=2 SV=1)

HSP 1 Score: 282.0 bits (720), Expect = 8.5e-75
Identity = 147/396 (37.12%), Postives = 223/396 (56.31%), Query Frame = 0


Query: 6   SLKLPLIDFSN--LESGGPKWELAKAQVKEALEEFGCFEASFDKVPVEVRKGLFEALEEL 65
           SL+LPLI+ ++  LE G  KW   ++ V++ALE+FGCFEAS+DKV +E+++ + + +EEL
Sbjct: 5   SLQLPLINLADKTLEPGSSKWAEVRSDVRKALEDFGCFEASYDKVSLELQESIMKTMEEL 64

Query: 66  FNLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTIPQKVQDFTNILWP--QGNPT 125
           F LP+ETK RNV  KP+ GY+    ++   ES+G+ ++ I + + +FT  LWP   GN  
Sbjct: 65  FALPVETKQRNVCPKPYVGYLNHNNLS---ESLGISNANILENINEFTQQLWPHGDGNEN 124

Query: 126 FSKVMETYSQQLAELDEMVRRMVLESLGVEKYLEEHLESTNYLLRVMKYKGTE------- 185
            SK ++ ++++L E+D MVRRMV+ES G+EKY+++HL+ST Y    +   G E       
Sbjct: 125 ISKTIQLFAEKLVEIDVMVRRMVMESFGIEKYIDDHLKSTAYATDELNIVGVEPNVGVKV 184

Query: 186 ------------------------------------------------------------ 245
                                                                       
Sbjct: 185 NADISDDVNANASVNAGVGANVNADTGVNDNLNVDANVAVGGGVNANTDLGVGVNVNSNV 244

Query: 246 ---------------SNYETKIGLHSHTDKNIVTILYQNHVQGLQVKTKDGKWINFQPSP 305
                           N E K+GL  HTDKN+ T+L+Q+ ++GL+VKTKD KWI  +PSP
Sbjct: 245 AVNAKTGGDDVEANDDNEEKKLGLPCHTDKNLFTVLFQHEIEGLEVKTKDEKWIRVKPSP 304

Query: 306 DSFVAMIGDSFHAWTNGRLHSPYHRVMMSGEE-VRYSAGLFSIPKAGYIVKAPEELVDEQ 315
           ++F+ + GDS  A  NGR+ +PYHRV ++ ++  RY+A +F+ PK  Y+++AP+ELVDE+
Sbjct: 305 NTFIVIAGDSLCALMNGRIRAPYHRVRVTEKKRTRYTAAIFTCPKPDYVIEAPKELVDEK 364

[Close]

BLAST of CSPI04G00590 vs. ExPASy Swiss-Prot
Match: Q945B5 (2-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thaliana OX=3702 GN=AOP2 PE=2 SV=1)

HSP 1 Score: 278.9 bits (712), Expect = 7.2e-74
Identity = 150/429 (34.97%), Postives = 228/429 (53.15%), Query Frame = 0


Query: 6   SLKLPLIDFSN--LESGGPKWELAKAQVKEALEEFGCFEASFDKVPVEVRKGLFEALEEL 65
           SL+LPLI+ ++  LE G  KW   ++ V++ALE+FGCFEAS+DKV +E+++ + + +EEL
Sbjct: 5   SLQLPLINLADKTLEPGSSKWAEVRSDVRKALEDFGCFEASYDKVSLELQESIMKTMEEL 64

Query: 66  FNLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTIPQKVQDFTNILWP--QGNPT 125
           F LP+ETK RNV  KP+ GY+    ++   ES+G+ ++ I + V +FT  LWP   GN  
Sbjct: 65  FALPVETKQRNVCPKPYVGYLNHNNLS---ESLGISNANILENVNEFTQQLWPDGDGNEN 124

Query: 126 FSKVMETYSQQLAELDEMVRRMVLESLGVEKYLEEHLESTNYLLRVMKY----------- 185
            SK ++ ++++L E+D MVRRMV+ES G+EKY+++HL+ST Y +R+MKY           
Sbjct: 125 ISKTIQLFAEKLVEIDVMVRRMVMESFGIEKYIDDHLKSTEYRMRLMKYIAPPEGDANTT 184

Query: 186 ------------------------------------------------------------ 245
                                                                       
Sbjct: 185 VDDYADLLAKLNIDGVEPNVGVKVNADISDDVNANPSVNAGVGANVNADTGVNDNLNVDA 244

Query: 246 --------------------------------------------KGTESNYETKIGLHSH 305
                                                       +  + N E K+GL  H
Sbjct: 245 EANGDANIAVGGGVNANTDLGVGVNVNSNVAVNAKTGATSGDDVEANDDNEEKKLGLPCH 304

Query: 306 TDKNIVTILYQNHVQGLQVKTKDGKWINFQPSPDSFVAMIGDSFHAWTNGRLHSPYHRVM 315
           TDKN+ T+L+Q+ ++GL+VKTKD KWI  +PSP++F+ + GDS  A  NGR+ +PYHRV 
Sbjct: 305 TDKNLFTVLFQHEIEGLEVKTKDEKWIRVKPSPNTFIVIAGDSLCALMNGRIRAPYHRVR 364

[Close]

BLAST of CSPI04G00590 vs. ExPASy TrEMBL
Match: A0A1S3BYJ6 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucumis melo OX=3656 GN=LOC103494791 PE=3 SV=1)

HSP 1 Score: 625.2 bits (1611), Expect = 1.5e-175
Identity = 301/314 (95.86%), Postives = 310/314 (98.73%), Query Frame = 0


Query: 1   MDSESSLKLPLIDFSNLESGGPKWELAKAQVKEALEEFGCFEASFDKVPVEVRKGLFEAL 60
           MD ESSLKLPLIDFSNLESGGPKWELAKAQVKEALEEFGCFEASFDKVP+EVRKGLFEAL
Sbjct: 1   MDGESSLKLPLIDFSNLESGGPKWELAKAQVKEALEEFGCFEASFDKVPIEVRKGLFEAL 60

Query: 61  EELFNLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTIPQKVQDFTNILWPQGNP 120
           EELFNLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTIPQKVQDFTNILWPQGNP
Sbjct: 61  EELFNLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTIPQKVQDFTNILWPQGNP 120

Query: 121 TFSKVMETYSQQLAELDEMVRRMVLESLGVEKYLEEHLESTNYLLRVMKYKGTESNYETK 180
           TFSKV+ETYSQQL+ELDEMVRRMVLESLGVEKY+EEHLESTNYLLRVMKYKGT+ + ETK
Sbjct: 121 TFSKVIETYSQQLSELDEMVRRMVLESLGVEKYMEEHLESTNYLLRVMKYKGTDQSNETK 180

Query: 181 IGLHSHTDKNIVTILYQNHVQGLQVKTKDGKWINFQPSPDSFVAMIGDSFHAWTNGRLHS 240
           IGLHSHTDKNIVTILYQNHV+GLQV+TKDGKWINFQPSP SFVAMIGDSFHAWTNGRLHS
Sbjct: 181 IGLHSHTDKNIVTILYQNHVEGLQVQTKDGKWINFQPSPHSFVAMIGDSFHAWTNGRLHS 240

Query: 241 PYHRVMMSGEEVRYSAGLFSIPKAGYIVKAPEELVDEQHPLLFNPFDHVQFLQFYYTEAG 300
           PYHRVMMSGEEVRYSAGLFSIPKAGYIVKAPEELVDEQHPLLFNPFDHVQFLQFYYTEAG
Sbjct: 241 PYHRVMMSGEEVRYSAGLFSIPKAGYIVKAPEELVDEQHPLLFNPFDHVQFLQFYYTEAG 300

Query: 301 QKAQSALKTYCGAT 315
           QKAQSALKT+CGAT
Sbjct: 301 QKAQSALKTFCGAT 314

[Close]

BLAST of CSPI04G00590 vs. ExPASy TrEMBL
Match: A0A6J1F3R6 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita moschata OX=3662 GN=LOC111441851 PE=3 SV=1)

HSP 1 Score: 571.6 bits (1472), Expect = 2.0e-159
Identity = 271/313 (86.58%), Postives = 300/313 (95.85%), Query Frame = 0


Query: 1   MDSESSLKLPLIDFSNLESGGPKWELAKAQVKEALEEFGCFEASFDKVPVEVRKGLFEAL 60
           MDSE SLKLP IDFSNLE+G PKWELAKA VKEAL+EFGCFEASFDKV  EVRKGLFEAL
Sbjct: 1   MDSEPSLKLPHIDFSNLEAGSPKWELAKALVKEALQEFGCFEASFDKVADEVRKGLFEAL 60

Query: 61  EELFNLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTIPQKVQDFTNILWPQGNP 120
           EELF+LPL+TKLRNVS+KPFHGYVGQYPMAPLFESMGVDDSTIP+KVQ F NI WPQGNP
Sbjct: 61  EELFDLPLQTKLRNVSKKPFHGYVGQYPMAPLFESMGVDDSTIPEKVQSFANIFWPQGNP 120

Query: 121 TFSKVMETYSQQLAELDEMVRRMVLESLGVEKYLEEHLESTNYLLRVMKYKGTESNYETK 180
           TFSKV+E+YSQQL+ELD+MVRRMV+ESLGVEKY+EEHL+STNYLLRVMKYKG ESN +T+
Sbjct: 121 TFSKVIESYSQQLSELDKMVRRMVVESLGVEKYMEEHLDSTNYLLRVMKYKGPESN-DTQ 180

Query: 181 IGLHSHTDKNIVTILYQNHVQGLQVKTKDGKWINFQPSPDSFVAMIGDSFHAWTNGRLHS 240
           +GLHSHTDKNIVTILYQNHV+GLQV+TKDGKWI+FQPSP+SFV MIGDSFHAWTNGRL+S
Sbjct: 181 LGLHSHTDKNIVTILYQNHVEGLQVQTKDGKWISFQPSPNSFVVMIGDSFHAWTNGRLYS 240

Query: 241 PYHRVMMSGEEVRYSAGLFSIPKAGYIVKAPEELVDEQHPLLFNPFDHVQFLQFYYTEAG 300
           PYHRV+MSGE+ RYSAGLFSIPKAGY++KAP+ELVDE+HPLLFNPFDHV+FLQFYYTEAG
Sbjct: 241 PYHRVVMSGEKARYSAGLFSIPKAGYVIKAPKELVDEEHPLLFNPFDHVEFLQFYYTEAG 300

Query: 301 QKAQSALKTYCGA 314
           Q+AQSALKTYCGA
Sbjct: 301 QRAQSALKTYCGA 312

[Close]

BLAST of CSPI04G00590 vs. ExPASy TrEMBL
Match: A0A6J1J6J6 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita maxima OX=3661 GN=LOC111482215 PE=3 SV=1)

HSP 1 Score: 570.9 bits (1470), Expect = 3.4e-159
Identity = 271/313 (86.58%), Postives = 300/313 (95.85%), Query Frame = 0


Query: 1   MDSESSLKLPLIDFSNLESGGPKWELAKAQVKEALEEFGCFEASFDKVPVEVRKGLFEAL 60
           MDSE SLKLP IDFSNLE+G PKWELAKA VKEAL+EFGCFEASFDKV  EVRKGLFEAL
Sbjct: 1   MDSEPSLKLPHIDFSNLEAGSPKWELAKALVKEALQEFGCFEASFDKVADEVRKGLFEAL 60

Query: 61  EELFNLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTIPQKVQDFTNILWPQGNP 120
           EELF+LPL+TKLRNVS+KPFHGYVGQYPMAPLFESMGVDDSTIP+KVQ F NI WPQGNP
Sbjct: 61  EELFDLPLQTKLRNVSKKPFHGYVGQYPMAPLFESMGVDDSTIPEKVQSFANIFWPQGNP 120

Query: 121 TFSKVMETYSQQLAELDEMVRRMVLESLGVEKYLEEHLESTNYLLRVMKYKGTESNYETK 180
           TFSKV+E+YSQQL+ELD+MVRRMV+ESLGVEKY+EEHL+STNYLLRVMKYKG ESN +T+
Sbjct: 121 TFSKVIESYSQQLSELDKMVRRMVVESLGVEKYMEEHLDSTNYLLRVMKYKGPESN-DTQ 180

Query: 181 IGLHSHTDKNIVTILYQNHVQGLQVKTKDGKWINFQPSPDSFVAMIGDSFHAWTNGRLHS 240
           +GLHSHTDKNIVTILYQNHV+GLQV+TKDGKWI+FQPSP+SFV MIGDSFHAWTNGRL+S
Sbjct: 181 LGLHSHTDKNIVTILYQNHVEGLQVQTKDGKWISFQPSPNSFVVMIGDSFHAWTNGRLYS 240

Query: 241 PYHRVMMSGEEVRYSAGLFSIPKAGYIVKAPEELVDEQHPLLFNPFDHVQFLQFYYTEAG 300
           PYHRV+MSGE+ RYSAGLFSIPKAGY++KAP+ELVDE+HPLLFNPFDHV+FLQFYYTEAG
Sbjct: 241 PYHRVVMSGEKARYSAGLFSIPKAGYMIKAPKELVDEEHPLLFNPFDHVEFLQFYYTEAG 300

Query: 301 QKAQSALKTYCGA 314
           Q+AQSALKTYCGA
Sbjct: 301 QRAQSALKTYCGA 312

[Close]

BLAST of CSPI04G00590 vs. ExPASy TrEMBL
Match: A0A6J1CQP5 (probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Momordica charantia OX=3673 GN=LOC111013275 PE=3 SV=1)

HSP 1 Score: 553.1 bits (1424), Expect = 7.3e-154
Identity = 264/313 (84.35%), Postives = 292/313 (93.29%), Query Frame = 0


Query: 1   MDSESSLKLPLIDFSNLESGGPKWELAKAQVKEALEEFGCFEASFDKVPVEVRKGLFEAL 60
           MD ESSLKLPLIDFSNLE G PKWE+AKA VK+ALEEFGCFEASF+KV +E RKGLFEAL
Sbjct: 1   MDCESSLKLPLIDFSNLELGSPKWEMAKALVKKALEEFGCFEASFEKVLLEARKGLFEAL 60

Query: 61  EELFNLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTIPQKVQDFTNILWPQGNP 120
           EELFNLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGV+DST+ +KVQ FTNILWPQGNP
Sbjct: 61  EELFNLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVEDSTMLEKVQTFTNILWPQGNP 120

Query: 121 TFSKVMETYSQQLAELDEMVRRMVLESLGVEKYLEEHLESTNYLLRVMKYKGTESNYETK 180
           TFS+V+E+YS+QL+ELD++VRRM+LESLGVEKYLEEHLESTNYLLRVMKYKG +S  ETK
Sbjct: 121 TFSQVIESYSKQLSELDQIVRRMILESLGVEKYLEEHLESTNYLLRVMKYKGPQSK-ETK 180

Query: 181 IGLHSHTDKNIVTILYQNHVQGLQVKTKDGKWINFQPSPDSFVAMIGDSFHAWTNGRLHS 240
           IGLH HTDKN VTILYQN V+GLQ++TKDG WI+F+PSP SFVAMIGDS HAWTNGRLHS
Sbjct: 181 IGLHPHTDKNFVTILYQNQVEGLQIQTKDGTWISFKPSPQSFVAMIGDSLHAWTNGRLHS 240

Query: 241 PYHRVMMSGEEVRYSAGLFSIPKAGYIVKAPEELVDEQHPLLFNPFDHVQFLQFYYTEAG 300
           P HRVMMSG+E RYSAGLFS+PKAGYI+KAP+ELVDE+HPLLF PFDHV+FLQFYYT+AG
Sbjct: 241 PRHRVMMSGKEARYSAGLFSVPKAGYIIKAPKELVDEEHPLLFKPFDHVEFLQFYYTQAG 300

Query: 301 QKAQSALKTYCGA 314
           Q AQSALKTYCGA
Sbjct: 301 QTAQSALKTYCGA 312

[Close]

BLAST of CSPI04G00590 vs. ExPASy TrEMBL
Match: M5WXX0 (Fe2OG dioxygenase domain-containing protein OS=Prunus persica OX=3760 GN=PRUPE_3G075700 PE=3 SV=1)

HSP 1 Score: 492.7 bits (1267), Expect = 1.2e-135
Identity = 228/314 (72.61%), Postives = 275/314 (87.58%), Query Frame = 0


Query: 1   MDSESSLKLPLIDFSN--LESGGPKWELAKAQVKEALEEFGCFEASFDKVPVEVRKGLFE 60
           M SE+ LKLP+IDFSN  L  G  +W   +AQV  ALEE+GCFEA F+KVP  +RK +F 
Sbjct: 1   MGSETPLKLPVIDFSNQSLTPGTTEWNTVRAQVHNALEEYGCFEALFNKVPPHIRKSIFP 60

Query: 61  ALEELFNLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTIPQKVQDFTNILWPQG 120
           ++EELF+LPL+TKL+NVS+KPFHGYVGQYPM PL+ESMGVDD+ + ++VQ  TN LWPQG
Sbjct: 61  SIEELFDLPLQTKLQNVSKKPFHGYVGQYPMVPLYESMGVDDANVYEQVQSLTNTLWPQG 120

Query: 121 NPTFSKVMETYSQQLAELDEMVRRMVLESLGVEKYLEEHLESTNYLLRVMKYKGTESNYE 180
           NP+F K ++++S+Q++ELD+++RRM+LESLG+EKYLEEHLESTNYLLRVMKYKG ++N E
Sbjct: 121 NPSFCKTIQSFSEQVSELDQIIRRMILESLGLEKYLEEHLESTNYLLRVMKYKGPQTN-E 180

Query: 181 TKIGLHSHTDKNIVTILYQNHVQGLQVKTKDGKWINFQPSPDSFVAMIGDSFHAWTNGRL 240
           TK+GL++HTDKNIVTILYQN V GL+V+ KDGKWIN +PSPDSF+AMIGDS +AWTNGRL
Sbjct: 181 TKLGLNAHTDKNIVTILYQNQVDGLEVQNKDGKWINVKPSPDSFIAMIGDSLYAWTNGRL 240

Query: 241 HSPYHRVMMSGEEVRYSAGLFSIPKAGYIVKAPEELVDEQHPLLFNPFDHVQFLQFYYTE 300
           HSPYHRVMMSG EVRYS GLFSIPK GYI+KAPEE+VDE+HPLLF PFDHVQFL FYY+E
Sbjct: 241 HSPYHRVMMSGNEVRYSTGLFSIPKEGYIIKAPEEVVDEEHPLLFKPFDHVQFLGFYYSE 300

Query: 301 AGQKAQSALKTYCG 313
           AGQ+A SALKTYCG
Sbjct: 301 AGQRAHSALKTYCG 313

[Close]

BLAST of CSPI04G00590 vs. NCBI nr
Match: XP_004152262.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis sativus] >KAE8649036.1 hypothetical protein Csa_014516 [Cucumis sativus])

HSP 1 Score: 645.6 bits (1664), Expect = 2.2e-181
Identity = 314/314 (100.00%), Postives = 314/314 (100.00%), Query Frame = 0


Query: 1   MDSESSLKLPLIDFSNLESGGPKWELAKAQVKEALEEFGCFEASFDKVPVEVRKGLFEAL 60
           MDSESSLKLPLIDFSNLESGGPKWELAKAQVKEALEEFGCFEASFDKVPVEVRKGLFEAL
Sbjct: 1   MDSESSLKLPLIDFSNLESGGPKWELAKAQVKEALEEFGCFEASFDKVPVEVRKGLFEAL 60

Query: 61  EELFNLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTIPQKVQDFTNILWPQGNP 120
           EELFNLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTIPQKVQDFTNILWPQGNP
Sbjct: 61  EELFNLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTIPQKVQDFTNILWPQGNP 120

Query: 121 TFSKVMETYSQQLAELDEMVRRMVLESLGVEKYLEEHLESTNYLLRVMKYKGTESNYETK 180
           TFSKVMETYSQQLAELDEMVRRMVLESLGVEKYLEEHLESTNYLLRVMKYKGTESNYETK
Sbjct: 121 TFSKVMETYSQQLAELDEMVRRMVLESLGVEKYLEEHLESTNYLLRVMKYKGTESNYETK 180

Query: 181 IGLHSHTDKNIVTILYQNHVQGLQVKTKDGKWINFQPSPDSFVAMIGDSFHAWTNGRLHS 240
           IGLHSHTDKNIVTILYQNHVQGLQVKTKDGKWINFQPSPDSFVAMIGDSFHAWTNGRLHS
Sbjct: 181 IGLHSHTDKNIVTILYQNHVQGLQVKTKDGKWINFQPSPDSFVAMIGDSFHAWTNGRLHS 240

Query: 241 PYHRVMMSGEEVRYSAGLFSIPKAGYIVKAPEELVDEQHPLLFNPFDHVQFLQFYYTEAG 300
           PYHRVMMSGEEVRYSAGLFSIPKAGYIVKAPEELVDEQHPLLFNPFDHVQFLQFYYTEAG
Sbjct: 241 PYHRVMMSGEEVRYSAGLFSIPKAGYIVKAPEELVDEQHPLLFNPFDHVQFLQFYYTEAG 300

Query: 301 QKAQSALKTYCGAT 315
           QKAQSALKTYCGAT
Sbjct: 301 QKAQSALKTYCGAT 314

[Close]

BLAST of CSPI04G00590 vs. NCBI nr
Match: XP_008454372.1 (PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis melo])

HSP 1 Score: 625.2 bits (1611), Expect = 3.1e-175
Identity = 301/314 (95.86%), Postives = 310/314 (98.73%), Query Frame = 0


Query: 1   MDSESSLKLPLIDFSNLESGGPKWELAKAQVKEALEEFGCFEASFDKVPVEVRKGLFEAL 60
           MD ESSLKLPLIDFSNLESGGPKWELAKAQVKEALEEFGCFEASFDKVP+EVRKGLFEAL
Sbjct: 1   MDGESSLKLPLIDFSNLESGGPKWELAKAQVKEALEEFGCFEASFDKVPIEVRKGLFEAL 60

Query: 61  EELFNLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTIPQKVQDFTNILWPQGNP 120
           EELFNLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTIPQKVQDFTNILWPQGNP
Sbjct: 61  EELFNLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTIPQKVQDFTNILWPQGNP 120

Query: 121 TFSKVMETYSQQLAELDEMVRRMVLESLGVEKYLEEHLESTNYLLRVMKYKGTESNYETK 180
           TFSKV+ETYSQQL+ELDEMVRRMVLESLGVEKY+EEHLESTNYLLRVMKYKGT+ + ETK
Sbjct: 121 TFSKVIETYSQQLSELDEMVRRMVLESLGVEKYMEEHLESTNYLLRVMKYKGTDQSNETK 180

Query: 181 IGLHSHTDKNIVTILYQNHVQGLQVKTKDGKWINFQPSPDSFVAMIGDSFHAWTNGRLHS 240
           IGLHSHTDKNIVTILYQNHV+GLQV+TKDGKWINFQPSP SFVAMIGDSFHAWTNGRLHS
Sbjct: 181 IGLHSHTDKNIVTILYQNHVEGLQVQTKDGKWINFQPSPHSFVAMIGDSFHAWTNGRLHS 240

Query: 241 PYHRVMMSGEEVRYSAGLFSIPKAGYIVKAPEELVDEQHPLLFNPFDHVQFLQFYYTEAG 300
           PYHRVMMSGEEVRYSAGLFSIPKAGYIVKAPEELVDEQHPLLFNPFDHVQFLQFYYTEAG
Sbjct: 241 PYHRVMMSGEEVRYSAGLFSIPKAGYIVKAPEELVDEQHPLLFNPFDHVQFLQFYYTEAG 300

Query: 301 QKAQSALKTYCGAT 315
           QKAQSALKT+CGAT
Sbjct: 301 QKAQSALKTFCGAT 314

[Close]

BLAST of CSPI04G00590 vs. NCBI nr
Match: XP_038906019.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1.2 [Benincasa hispida])

HSP 1 Score: 587.4 bits (1513), Expect = 7.3e-164
Identity = 280/314 (89.17%), Postives = 303/314 (96.50%), Query Frame = 0


Query: 1   MDSESSLKLPLIDFSNLESGGPKWELAKAQVKEALEEFGCFEASFDKVPVEVRKGLFEAL 60
           MDSE+SLKLP IDFSNLE G PKWE+ KAQVKEALEEFGCFEASFDKVP+EVRKGLFEAL
Sbjct: 1   MDSETSLKLPSIDFSNLELGSPKWEMVKAQVKEALEEFGCFEASFDKVPLEVRKGLFEAL 60

Query: 61  EELFNLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTIPQKVQDFTNILWPQGNP 120
           EELFNLPL+TKLRNVS+KPFHGYVGQYP+APLFESMGVDDST+P+KVQ FTNILWPQGN 
Sbjct: 61  EELFNLPLQTKLRNVSKKPFHGYVGQYPLAPLFESMGVDDSTLPEKVQSFTNILWPQGNS 120

Query: 121 TFSKVMETYSQQLAELDEMVRRMVLESLGVEKYLEEHLESTNYLLRVMKYKGTESNYETK 180
           TFSKV+E+YSQQL+ELD+MVRRMVLESLGVEKYLEEHLESTNYLLRVMKYKG +SN ET+
Sbjct: 121 TFSKVIESYSQQLSELDQMVRRMVLESLGVEKYLEEHLESTNYLLRVMKYKGPQSN-ETQ 180

Query: 181 IGLHSHTDKNIVTILYQNHVQGLQVKTKDGKWINFQPSPDSFVAMIGDSFHAWTNGRLHS 240
           +GLHSHTDKNIVTILYQNHV+GLQV+TKDGKWINFQPSP+SFVAMIGDS HAWTNGRLHS
Sbjct: 181 LGLHSHTDKNIVTILYQNHVEGLQVQTKDGKWINFQPSPNSFVAMIGDSLHAWTNGRLHS 240

Query: 241 PYHRVMMSGEEVRYSAGLFSIPKAGYIVKAPEELVDEQHPLLFNPFDHVQFLQFYYTEAG 300
           PYHRVMMSG E RYSAGLFSIPKAGYI+KAP+ELVDEQHPLLFNPFDHV+FLQFYYTEAG
Sbjct: 241 PYHRVMMSGGEARYSAGLFSIPKAGYIIKAPKELVDEQHPLLFNPFDHVEFLQFYYTEAG 300

Query: 301 QKAQSALKTYCGAT 315
           Q+AQSALKT+CGAT
Sbjct: 301 QRAQSALKTFCGAT 313

[Close]

BLAST of CSPI04G00590 vs. NCBI nr
Match: XP_022934777.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita moschata])

HSP 1 Score: 571.6 bits (1472), Expect = 4.1e-159
Identity = 271/313 (86.58%), Postives = 300/313 (95.85%), Query Frame = 0


Query: 1   MDSESSLKLPLIDFSNLESGGPKWELAKAQVKEALEEFGCFEASFDKVPVEVRKGLFEAL 60
           MDSE SLKLP IDFSNLE+G PKWELAKA VKEAL+EFGCFEASFDKV  EVRKGLFEAL
Sbjct: 1   MDSEPSLKLPHIDFSNLEAGSPKWELAKALVKEALQEFGCFEASFDKVADEVRKGLFEAL 60

Query: 61  EELFNLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTIPQKVQDFTNILWPQGNP 120
           EELF+LPL+TKLRNVS+KPFHGYVGQYPMAPLFESMGVDDSTIP+KVQ F NI WPQGNP
Sbjct: 61  EELFDLPLQTKLRNVSKKPFHGYVGQYPMAPLFESMGVDDSTIPEKVQSFANIFWPQGNP 120

Query: 121 TFSKVMETYSQQLAELDEMVRRMVLESLGVEKYLEEHLESTNYLLRVMKYKGTESNYETK 180
           TFSKV+E+YSQQL+ELD+MVRRMV+ESLGVEKY+EEHL+STNYLLRVMKYKG ESN +T+
Sbjct: 121 TFSKVIESYSQQLSELDKMVRRMVVESLGVEKYMEEHLDSTNYLLRVMKYKGPESN-DTQ 180

Query: 181 IGLHSHTDKNIVTILYQNHVQGLQVKTKDGKWINFQPSPDSFVAMIGDSFHAWTNGRLHS 240
           +GLHSHTDKNIVTILYQNHV+GLQV+TKDGKWI+FQPSP+SFV MIGDSFHAWTNGRL+S
Sbjct: 181 LGLHSHTDKNIVTILYQNHVEGLQVQTKDGKWISFQPSPNSFVVMIGDSFHAWTNGRLYS 240

Query: 241 PYHRVMMSGEEVRYSAGLFSIPKAGYIVKAPEELVDEQHPLLFNPFDHVQFLQFYYTEAG 300
           PYHRV+MSGE+ RYSAGLFSIPKAGY++KAP+ELVDE+HPLLFNPFDHV+FLQFYYTEAG
Sbjct: 241 PYHRVVMSGEKARYSAGLFSIPKAGYVIKAPKELVDEEHPLLFNPFDHVEFLQFYYTEAG 300

Query: 301 QKAQSALKTYCGA 314
           Q+AQSALKTYCGA
Sbjct: 301 QRAQSALKTYCGA 312

[Close]

BLAST of CSPI04G00590 vs. NCBI nr
Match: XP_022983670.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita maxima])

HSP 1 Score: 570.9 bits (1470), Expect = 7.0e-159
Identity = 271/313 (86.58%), Postives = 300/313 (95.85%), Query Frame = 0


Query: 1   MDSESSLKLPLIDFSNLESGGPKWELAKAQVKEALEEFGCFEASFDKVPVEVRKGLFEAL 60
           MDSE SLKLP IDFSNLE+G PKWELAKA VKEAL+EFGCFEASFDKV  EVRKGLFEAL
Sbjct: 1   MDSEPSLKLPHIDFSNLEAGSPKWELAKALVKEALQEFGCFEASFDKVADEVRKGLFEAL 60

Query: 61  EELFNLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTIPQKVQDFTNILWPQGNP 120
           EELF+LPL+TKLRNVS+KPFHGYVGQYPMAPLFESMGVDDSTIP+KVQ F NI WPQGNP
Sbjct: 61  EELFDLPLQTKLRNVSKKPFHGYVGQYPMAPLFESMGVDDSTIPEKVQSFANIFWPQGNP 120

Query: 121 TFSKVMETYSQQLAELDEMVRRMVLESLGVEKYLEEHLESTNYLLRVMKYKGTESNYETK 180
           TFSKV+E+YSQQL+ELD+MVRRMV+ESLGVEKY+EEHL+STNYLLRVMKYKG ESN +T+
Sbjct: 121 TFSKVIESYSQQLSELDKMVRRMVVESLGVEKYMEEHLDSTNYLLRVMKYKGPESN-DTQ 180

Query: 181 IGLHSHTDKNIVTILYQNHVQGLQVKTKDGKWINFQPSPDSFVAMIGDSFHAWTNGRLHS 240
           +GLHSHTDKNIVTILYQNHV+GLQV+TKDGKWI+FQPSP+SFV MIGDSFHAWTNGRL+S
Sbjct: 181 LGLHSHTDKNIVTILYQNHVEGLQVQTKDGKWISFQPSPNSFVVMIGDSFHAWTNGRLYS 240

Query: 241 PYHRVMMSGEEVRYSAGLFSIPKAGYIVKAPEELVDEQHPLLFNPFDHVQFLQFYYTEAG 300
           PYHRV+MSGE+ RYSAGLFSIPKAGY++KAP+ELVDE+HPLLFNPFDHV+FLQFYYTEAG
Sbjct: 241 PYHRVVMSGEKARYSAGLFSIPKAGYMIKAPKELVDEEHPLLFNPFDHVEFLQFYYTEAG 300

Query: 301 QKAQSALKTYCGA 314
           Q+AQSALKTYCGA
Sbjct: 301 QRAQSALKTYCGA 312

[Close]

BLAST of CSPI04G00590 vs. TAIR 10
Match: AT1G52820.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 455.3 bits (1170), Expect = 4.0e-128
Identity = 210/308 (68.18%), Postives = 265/308 (86.04%), Query Frame = 0


Query: 7   LKLPLIDFS--NLESGGPKWELAKAQVKEALEEFGCFEASFDKVPVEVRKGLFEALEELF 66
           L+LP+IDFS  NL+ G P+W+L +A V++AL+++G FEASFD++P E+RK +F ALEELF
Sbjct: 10  LRLPVIDFSNKNLKPGEPEWDLTRADVQKALQDYGYFEASFDRIPFELRKSVFGALEELF 69

Query: 67  NLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTIPQKVQDFTNILWPQGNPTFSK 126
           +LPL+TKLRNVS+KPFHGYVGQYPM PL+ESMG+DDS I +KV  FT  LWPQGN +FS 
Sbjct: 70  DLPLQTKLRNVSKKPFHGYVGQYPMVPLYESMGIDDSDIAEKVDAFTEKLWPQGNISFST 129

Query: 127 VMETYSQQLAELDEMVRRMVLESLGVEKYLEEHLESTNYLLRVMKYKGTESNYETKIGLH 186
            ++++S++L+ELD  +RRM++ES G++KY++EHL STNYLLRVMKYKG ++  ETK+GL+
Sbjct: 130 TIQSFSKKLSELDITIRRMIMESFGLDKYIDEHLHSTNYLLRVMKYKGPDTE-ETKVGLN 189

Query: 187 SHTDKNIVTILYQNHVQGLQVKTKDGKWINFQPSPDSFVAMIGDSFHAWTNGRLHSPYHR 246
           +HTDKNIVTILYQNHV+GL+V+TKD  WI  +P+ DSF  MIGDS +A  NGRLHSPYHR
Sbjct: 190 AHTDKNIVTILYQNHVEGLEVQTKDKNWIKVKPTQDSFTVMIGDSLYALLNGRLHSPYHR 249

Query: 247 VMMSGEEVRYSAGLFSIPKAGYIVKAPEELVDEQHPLLFNPFDHVQFLQFYYTEAGQKAQ 306
           VMM+G E RYS GLFSIPKAG+IV +P+ELVDE+HP LF PFDHV+FLQFYYTEAGQ++Q
Sbjct: 250 VMMTGTETRYSLGLFSIPKAGHIVSSPDELVDEEHPRLFKPFDHVEFLQFYYTEAGQRSQ 309

Query: 307 SALKTYCG 313
           SALKTYCG
Sbjct: 310 SALKTYCG 316

[Close]

BLAST of CSPI04G00590 vs. TAIR 10
Match: AT4G03070.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 361.7 bits (927), Expect = 6.0e-100
Identity = 175/313 (55.91%), Postives = 231/313 (73.80%), Query Frame = 0


Query: 6   SLKLPLIDFS--NLESGGPKWELAKAQVKEALEEFGCFEASFDKVPVEVRKGLFEALEEL 65
           S +LP+IDFS  NL+ G  KW+   A V +ALE++GCFEASFDK+ VE+ + +FEA+E+L
Sbjct: 11  SFQLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDL 70

Query: 66  FNLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTIPQKVQDFTNILWP-QGNPTF 125
           F LP+ TK RNVS KPFHGY+       L+ES+G++D+ + +KV DFT  LWP  GN + 
Sbjct: 71  FELPIPTKQRNVSSKPFHGYL----CHNLYESLGINDANVLEKVNDFTQQLWPDHGNKSI 130

Query: 126 SKVMETYSQQLAELDEMVRRMVLESLGVEKYLEEHLESTNYLLRVMKYKG-----TESNY 185
           S+ +  +S+QL ELD MVRRM++ES G+E Y++EHL ST YL R+MKY        + + 
Sbjct: 131 SETIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYTSPPDDDDDDDE 190

Query: 186 ETKIGLHSHTDKNIVTILYQNHVQGLQVKTKDGKWINFQPSPDSFVAMIGDSFHAWTNGR 245
           ETK+GL SHTDKNI+TIL+Q  V GL+VKTKD KWI  +PS DS + M+GDS  A  NGR
Sbjct: 191 ETKLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKPSQDSVLVMVGDSLCALLNGR 250

Query: 246 LHSPYHRVMMSGEEVRYSAGLFSIPKAGYIVKAPEELVDEQHPLLFNPFDHVQFLQFYYT 305
           LHSPYHRV+M+G++ RYS GLFSIPK G I+ +PEELVD++HP +F PF++  FL F+ T
Sbjct: 251 LHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHFFQT 310

Query: 306 EAGQKAQSALKTY 311
           EAG+ AQSAL  +
Sbjct: 311 EAGRIAQSALHAF 319

[Close]

BLAST of CSPI04G00590 vs. TAIR 10
Match: AT1G52800.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 290.8 bits (743), Expect = 1.3e-78
Identity = 140/308 (45.45%), Postives = 198/308 (64.29%), Query Frame = 0


Query: 8   KLPLIDFSNLESGGPK---WELAKAQVKEALEEFGCFEASFDKVPVEVRKGLFEALEELF 67
           K+P++D ++ +   P    W     +  EALEE+GCF A +D V  ++   +F A EELF
Sbjct: 9   KVPILDLTSQQELKPNTSTWRSVSREACEALEEYGCFLAVYDGVTQQLDDSIFAAAEELF 68

Query: 68  NLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTIPQKVQDFTNILWPQGNPTFSK 127
           +LP ETK +NV++KP+HGYVGQ P+ PL E +GVD  T  +  Q FT+++WPQGN  F  
Sbjct: 69  DLPTETKKKNVNEKPYHGYVGQMPVIPLHEGLGVDYVTNKEIAQRFTHLMWPQGNDRFCN 128

Query: 128 VMETYSQQLAELDEMVRRMVLESLGVEKYLEEHLESTNYLLRVMKYKGTESNYETKIGLH 187
            + T+S  +AELD +V RM+ E+ GVEK+ E H+ S  YLL+ +KY     +        
Sbjct: 129 TVHTFSNAVAELDRLVVRMIFENYGVEKHYESHVGSKTYLLKFLKYLAPPESISMP-AFP 188

Query: 188 SHTDKNIVTILYQNHVQGLQVKTKDGKWINFQPSPDSFVAMIGDSFHAWTNGRLHSPYHR 247
            HTDK  ++IL+QN V GL+VK+KDG+WI+ Q  P S+V M GD    W+N R+ S  HR
Sbjct: 189 QHTDKTFLSILHQNDVNGLEVKSKDGEWISLQLPPKSYVVMAGDISMGWSNDRIRSCEHR 248

Query: 248 VMMSGEEVRYSAGLFSIPKAGYIVKAPEELVDEQHPLLFNPFDHVQFLQFYYTEAGQKAQ 307
           V M G++ RY+ GLFS      +V  PEELVD++HPL++ PFD++  + FY T+ G++A 
Sbjct: 249 VTMEGDKTRYTLGLFSF--LTDLVSIPEELVDDKHPLMYKPFDNIALINFYTTKEGREAN 308

Query: 308 SALKTYCG 313
           S LK YCG
Sbjct: 309 STLKAYCG 313

[Close]

BLAST of CSPI04G00590 vs. TAIR 10
Match: AT1G52790.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 288.1 bits (736), Expect = 8.5e-78
Identity = 138/311 (44.37%), Postives = 210/311 (67.52%), Query Frame = 0


Query: 6   SLKLPLIDFS--NLESGGPKWELAKAQVKEALEEFGCFEASF-DKVPVEVRKGLFEALEE 65
           S K+P +DFS  +L+ G   WE  +  +++ALEE+GCF     DK P+++   +F +L +
Sbjct: 2   SPKIPTLDFSREDLKPGTKYWESTRENIRQALEEYGCFIIDLKDKTPLDLLDRVFGSLVD 61

Query: 66  LFNLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTIPQKVQDFTNILWPQGNPTF 125
           LF+LP +TK++N   KP +GYVGQ P  PL ES+G+D++T  +  + FT ++WPQGN  F
Sbjct: 62  LFDLPTQTKMKNKYDKPLNGYVGQIPALPLHESLGIDNATSLEATRSFTGLMWPQGNEHF 121

Query: 126 SKVMETYSQQLAELDEMVRRMVLESLGVEKYLEEHLESTNYLLRVMKYKGTESNYETKIG 185
           S+ +  Y++  AELD+MV RMV +S  VEKY + ++EST YLLRV+K +   +N    +G
Sbjct: 122 SECLYKYAEFAAELDQMVTRMVFQSYNVEKYYDPYIESTTYLLRVLKNR-APNNENPTLG 181

Query: 186 LHSHTDKNIVTILYQNHVQGLQVKTKDGKWINFQ-PSPDSFVAMIGDSFHAWTNGRLHSP 245
             +HTDK+  TIL+Q+ V GL+++T++G+ IN    SP  F+ + GD+  AW+N R+ SP
Sbjct: 182 FVTHTDKSFTTILHQDQVNGLEMETREGERININLSSPSLFMVVAGDALMAWSNDRVWSP 241

Query: 246 YHRVMMSGEEVRYSAGLFSIPKAGYIVKAPEELVDEQHPLLFNPFDHVQFLQFYYTEAGQ 305
            H+V++SGE  RYS G+F+       ++ PEEL+D QHPL++ PFDH+  L+FY T+ G 
Sbjct: 242 RHQVLVSGETDRYSLGMFAFNNG--TLQVPEELIDHQHPLMYKPFDHLGLLRFYRTDTGY 301

Query: 306 KAQSALKTYCG 313
           K++  +K YCG
Sbjct: 302 KSECPIKAYCG 309

[Close]

BLAST of CSPI04G00590 vs. TAIR 10
Match: AT1G28030.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 283.1 bits (723), Expect = 2.7e-76
Identity = 148/316 (46.84%), Postives = 209/316 (66.14%), Query Frame = 0


Query: 2   DSESSLKLPLIDFSN--LESGGPKWELAKAQVKEALEEFGCFEASFDKVPVEVRKGLFEA 61
           +S+  L LP+IDFSN  L+   P+W+L ++QV++ALEE+GCFEA FD   +E+RK LFE+
Sbjct: 6   ESQVPLSLPIIDFSNPDLKPETPEWDLVRSQVRKALEEYGCFEALFDGASMELRKALFES 65

Query: 62  LEELFNLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTIPQKVQDFTNILWPQGN 121
            +E+F+LPLETKL   +   + GY+   P  P+ E MG      P  V D T+ LWPQGN
Sbjct: 66  SKEVFDLPLETKLSTKTDVHYEGYL-TIPRVPIQEGMGFYGIDNPNVVNDLTHKLWPQGN 125

Query: 122 PTFSKVMETYSQQLAELDEMVRRMVLESLGVEKYLEEHLESTNYLLRVMKYKG-TESNYE 181
               K +++++++L EL+  VR M LES G+EKY+EEHL + N   +++KYKG ++ N E
Sbjct: 126 IFVGKNVQSFAEKLIELNLTVRTMTLESFGLEKYMEEHLNAANKHFQLLKYKGISDDNTE 185

Query: 182 TKIGLHSHTDKNIVTILYQNH-VQGLQVKTKDG-KWINFQPS-PDSFVAMIGDSFHAWTN 241
            KIG + H D++ +TIL QN  V GL++KTKDG +WI  +PS   SF+ M G S H   N
Sbjct: 186 NKIGFYPHIDRHFLTILCQNDAVDGLEIKTKDGEEWIKVKPSQASSFIVMAGASLHVLLN 245

Query: 242 GRLHSPYHRVMMSGEEVRYSAGLFSIPKAGYIVKAPEELVDEQHPLLFNPFDHVQFLQFY 301
           G +  P HRV+++G++ RY A LF+IPK G I+ APEE+VD++HP L+ PFD   FL+F 
Sbjct: 246 GGVFPPLHRVVITGKKDRYVAALFTIPKEGVIINAPEEMVDDEHPRLYKPFDFWGFLKFS 305

Query: 302 YTEAGQKAQSALKTYC 312
                +K  S L  YC
Sbjct: 306 NLPNARKDISDLTDYC 320

The following BLAST results are available for this feature:

BLAST of CSPI04G00590 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q945B6	1.7e-99	56.41	Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana OX=...	[more]
Q9ZTA3	8.5e-99	55.91	Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana OX=37...	[more]
Q944Z5	5.1e-96	56.29	Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis tha...	[more]
Q9ZTA2	8.5e-75	37.12	Probable inactive 2-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thali...	[more]
Q945B5	7.2e-74	34.97	2-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thaliana OX=3702 GN=AOP...	[more]

BLAST of CSPI04G00590 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3BYJ6	1.5e-175	95.86	probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucumis melo OX=3656 GN=LO...	[more]
A0A6J1F3R6	2.0e-159	86.58	probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita moschata OX=3662...	[more]
A0A6J1J6J6	3.4e-159	86.58	probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita maxima OX=3661 G...	[more]
A0A6J1CQP5	7.3e-154	84.35	probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Momordica charantia OX=3...	[more]
M5WXX0	1.2e-135	72.61	Fe2OG dioxygenase domain-containing protein OS=Prunus persica OX=3760 GN=PRUPE_3...	[more]

BLAST of CSPI04G00590 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004152262.1	2.2e-181	100.00	probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis sativus] >KAE8649036...	[more]
XP_008454372.1	3.1e-175	95.86	PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis melo]	[more]
XP_038906019.1	7.3e-164	89.17	probable 2-oxoglutarate-dependent dioxygenase AOP1.2 [Benincasa hispida]	[more]
XP_022934777.1	4.1e-159	86.58	probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita moschata]	[more]
XP_022983670.1	7.0e-159	86.58	probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita maxima]	[more]

BLAST of CSPI04G00590 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G52820.1	4.0e-128	68.18	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT4G03070.1	6.0e-100	55.91	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G52800.1	1.3e-78	45.45	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G52790.1	8.5e-78	44.37	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G28030.1	2.7e-76	46.84	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 126..146
None	No IPR available	PRINTS	PR00682	IPNSYNTHASE	coord: 222..248 score: 28.96 coord: 186..207 score: 51.65
None	No IPR available	PANTHER	PTHR47990:SF128	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN	coord: 4..312
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 4..312
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 5..300
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 9..85 e-value: 1.4E-12 score: 48.4
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 7..312 e-value: 3.6E-67 score: 228.9
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 170..259 e-value: 3.7E-19 score: 69.1
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 160..262 score: 10.140985

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI04G00590.1	CSPI04G00590.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009805	coumarin biosynthetic process
biological_process	GO:0002238	response to molecule of fungal origin
molecular_function	GO:0016706	2-oxoglutarate-dependent dioxygenase activity
molecular_function	GO:0046872	metal ion binding