CSPI04G00300 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI04G00300
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	trypsin inhibitor 1-like
Location	Chr4: 183790 .. 184072 (+)
RNA-Seq Expression	CSPI04G00300
Synteny	CSPI04G00300

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GATTAAGAAGAGAAAAAAAGAAAGAAAGAAAGGAATTGAAAAAATGGAGTGGAAGAAAATTGGAATGTTGGCTGTGGTGGGGATGCTGGTGATGGCAGCTTTGGTGGAGGATTGTCATGCAGCCATTGAATCAGAGTCTGTGAAAGGTTTGAATAAAAATGAATTGCCAAGAAAGATGATGAATGAAGAAACTAGAATGTGCCCTAGAATCCTTGAAGAGTGCACTACGGACGATGATTGCATGAATGATTGCATTTGCCTCTCCAATGGCTTTTGCGGTTGA

mRNA sequence

GATTAAGAAGAGAAAAAAAGAAAGAAAGAAAGGAATTGAAAAAATGGAGTGGAAGAAAATTGGAATGTTGGCTGTGGTGGGGATGCTGGTGATGGCAGCTTTGGTGGAGGATTGTCATGCAGCCATTGAATCAGAGTCTGTGAAAGGTTTGAATAAAAATGAATTGCCAAGAAAGATGATGAATGAAGAAACTAGAATGTGCCCTAGAATCCTTGAAGAGTGCACTACGGACGATGATTGCATGAATGATTGCATTTGCCTCTCCAATGGCTTTTGCGGTTGA

Coding sequence (CDS)

ATGGAGTGGAAGAAAATTGGAATGTTGGCTGTGGTGGGGATGCTGGTGATGGCAGCTTTGGTGGAGGATTGTCATGCAGCCATTGAATCAGAGTCTGTGAAAGGTTTGAATAAAAATGAATTGCCAAGAAAGATGATGAATGAAGAAACTAGAATGTGCCCTAGAATCCTTGAAGAGTGCACTACGGACGATGATTGCATGAATGATTGCATTTGCCTCTCCAATGGCTTTTGCGGTTGA

Protein sequence

MEWKKIGMLAVVGMLVMAALVEDCHAAIESESVKGLNKNELPRKMMNEETRMCPRILEECTTDDDCMNDCICLSNGFCG*

Homology

[Close]

BLAST of CSPI04G00300 vs. ExPASy Swiss-Prot
Match: P34950 (Trypsin inhibitor 5 OS=Luffa aegyptiaca OX=3670 PE=1 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 1.3e-08
Identity = 27/42 (64.29%), Postives = 32/42 (76.19%), Query Frame = 0


Query: 39 NELPRKMMN-EETRMCPRILEECTTDDDCMNDCICLSNGFCG 80
          N+LPRK+M+     +CPRIL  C TDDDCM DC CLSNG+CG
Sbjct: 22 NDLPRKIMSGRHGGICPRILMPCKTDDDCMLDCRCLSNGYCG 63

[Close]

BLAST of CSPI04G00300 vs. ExPASy Swiss-Prot
Match: Q43667 (Trypsin inhibitor 1 OS=Trichosanthes kirilowii OX=3677 PE=3 SV=1)

HSP 1 Score: 54.3 bits (129), Expect = 7.4e-07
Identity = 31/66 (46.97%), Postives = 36/66 (54.55%), Query Frame = 0


Query: 17 MAALVEDCHAAIESESVKGL---NKNELPRKMMNEETRMCPRILEECTTDDDCMNDCICL 76
          MAA VE   A   ++ V  L     NE   KMM E    CPRIL  C  +DDC+  C CL
Sbjct: 1  MAAFVESARAGAGADEVIQLVSDGVNEYSEKMM-EGVVACPRILMPCKVNDDCLRGCKCL 60

Query: 77 SNGFCG 80
          SNG+CG
Sbjct: 61 SNGYCG 65

[Close]

BLAST of CSPI04G00300 vs. ExPASy Swiss-Prot
Match: Q9S8I2 (Trypsin inhibitor 3 OS=Luffa aegyptiaca OX=3670 PE=1 SV=1)

HSP 1 Score: 54.3 bits (129), Expect = 7.4e-07
Identity = 20/29 (68.97%), Postives = 25/29 (86.21%), Query Frame = 0


Query: 51 RMCPRILEECTTDDDCMNDCICLSNGFCG 80
          R+CPRIL EC++D DC+ +CICL NGFCG
Sbjct: 1  RICPRILMECSSDSDCLAECICLENGFCG 29

[Close]

BLAST of CSPI04G00300 vs. ExPASy Swiss-Prot
Match: P25849 (Trypsin inhibitor 1 OS=Luffa aegyptiaca OX=3670 PE=1 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 3.7e-06
Identity = 19/29 (65.52%), Postives = 24/29 (82.76%), Query Frame = 0


Query: 51 RMCPRILEECTTDDDCMNDCICLSNGFCG 80
          R+CPRIL EC++D DC+ +CICL  GFCG
Sbjct: 1  RICPRILMECSSDSDCLAECICLEQGFCG 29

[Close]

BLAST of CSPI04G00300 vs. ExPASy Swiss-Prot
Match: P32041 (Trypsin inhibitor 3 OS=Cucumis melo var. conomon OX=3657 PE=1 SV=1)

HSP 1 Score: 50.1 bits (118), Expect = 1.4e-05
Identity = 18/29 (62.07%), Postives = 22/29 (75.86%), Query Frame = 0


Query: 51 RMCPRILEECTTDDDCMNDCICLSNGFCG 80
          RMCP+IL +C  D DC+ DC+CL  GFCG
Sbjct: 2  RMCPKILMKCKQDSDCLLDCVCLKEGFCG 30

[Close]

BLAST of CSPI04G00300 vs. ExPASy TrEMBL
Match: A0A0A0KT01 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G000805 PE=3 SV=1)

HSP 1 Score: 166.0 bits (419), Expect = 6.4e-38
Identity = 79/79 (100.00%), Postives = 79/79 (100.00%), Query Frame = 0


Query: 1  MEWKKIGMLAVVGMLVMAALVEDCHAAIESESVKGLNKNELPRKMMNEETRMCPRILEEC 60
          MEWKKIGMLAVVGMLVMAALVEDCHAAIESESVKGLNKNELPRKMMNEETRMCPRILEEC
Sbjct: 1  MEWKKIGMLAVVGMLVMAALVEDCHAAIESESVKGLNKNELPRKMMNEETRMCPRILEEC 60

Query: 61 TTDDDCMNDCICLSNGFCG 80
          TTDDDCMNDCICLSNGFCG
Sbjct: 61 TTDDDCMNDCICLSNGFCG 79

[Close]

BLAST of CSPI04G00300 vs. ExPASy TrEMBL
Match: A0A6J1JBL8 (trypsin inhibitor 1-like OS=Cucurbita maxima OX=3661 GN=LOC111482991 PE=3 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 5.7e-10
Identity = 41/82 (50.00%), Postives = 51/82 (62.20%), Query Frame = 0


Query: 1  MEWKKIGMLAVVGMLVMAALVEDCHAAIESESVKGL---NKNELPRKMMNEETRMCPRIL 60
          MEWK+  ++A+VG+L+MAA  E   AA+    V  L     N+ PRKMMN   + CPRIL
Sbjct: 1  MEWKRGALIAMVGLLLMAAFAES--AAVHGGEVIQLVSDGVNDFPRKMMN-GVQGCPRIL 60

Query: 61 EECTTDDDCMNDCICLSNGFCG 80
           EC  D DC+  CIC  NGFCG
Sbjct: 61 MECELDSDCLPGCICRPNGFCG 79

[Close]

BLAST of CSPI04G00300 vs. ExPASy TrEMBL
Match: A0A5D3E148 (Trypsin inhibitor 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G00640 PE=3 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 9.0e-08
Identity = 37/92 (40.22%), Postives = 52/92 (56.52%), Query Frame = 0


Query: 1  MEWKKIGMLAVVGMLVM-AALVEDCHAAIESESVKGL------NKNELPRKMM------N 60
          M+ KKI ++A+VGM++M AA+       ++ +    L        N  PRK+M       
Sbjct: 1  MDSKKIVVVAIVGMIIMLAAMSSSASFVVDDKDSTNLVSDGRDMNNGCPRKIMIKGGVFK 60

Query: 61 EETRMCPRILEECTTDDDCMNDCICLSNGFCG 80
          EE RMCP+IL +C  D DC+ DC+CL  GFCG
Sbjct: 61 EEQRMCPKILMKCKQDSDCLLDCVCLKEGFCG 92

[Close]

BLAST of CSPI04G00300 vs. ExPASy TrEMBL
Match: A0A0A0KY34 (Trypsin inhibitor 1 OS=Cucumis sativus OX=3659 GN=Csa_4G000810 PE=3 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 2.6e-07
Identity = 31/81 (38.27%), Postives = 47/81 (58.02%), Query Frame = 0


Query: 1  MEWKKIGMLAVVGMLVMAALVEDCHAAIESESVKGLNK--NELPRKMMNEETRMCPRILE 60
          MEWKKI ++A++GML+MA   E     ++ E ++ ++   NE  + +  +    CPRIL 
Sbjct: 1  MEWKKIALVAMMGMLLMATFTESVGLGVDEEIIQLVSDGVNEYSQNIGKDTAPGCPRILM 60

Query: 61 ECTTDDDCMNDCICLSNGFCG 80
          +C TD DC   C C   G+CG
Sbjct: 61 KCKTDSDCYPGCTCKPMGYCG 81

[Close]

BLAST of CSPI04G00300 vs. ExPASy TrEMBL
Match: A0A5D3E0W0 (Trypsin inhibitor 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G00600 PE=3 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 1.0e-06
Identity = 33/81 (40.74%), Postives = 47/81 (58.02%), Query Frame = 0


Query: 1  MEWKKIGMLAVVGMLVMAALVEDCHAAIESESVKGLNKNELPRKMMNEETRM--CPRILE 60
          MEWKKI ++A++GML+MA   E     ++ E +      +L    +NE +    CPRIL 
Sbjct: 1  MEWKKIALVAMMGMLLMATFTESVGFGVDEEII------QLVSDGVNEYSGHPGCPRILM 60

Query: 61 ECTTDDDCMNDCICLSNGFCG 80
          +C TD DC+  C C  NG+CG
Sbjct: 61 KCKTDRDCLAGCTCKRNGYCG 75

[Close]

BLAST of CSPI04G00300 vs. NCBI nr
Match: XP_031739937.1 (uncharacterized protein LOC105435333 [Cucumis sativus])

HSP 1 Score: 166.0 bits (419), Expect = 1.3e-37
Identity = 79/79 (100.00%), Postives = 79/79 (100.00%), Query Frame = 0


Query: 1  MEWKKIGMLAVVGMLVMAALVEDCHAAIESESVKGLNKNELPRKMMNEETRMCPRILEEC 60
          MEWKKIGMLAVVGMLVMAALVEDCHAAIESESVKGLNKNELPRKMMNEETRMCPRILEEC
Sbjct: 1  MEWKKIGMLAVVGMLVMAALVEDCHAAIESESVKGLNKNELPRKMMNEETRMCPRILEEC 60

Query: 61 TTDDDCMNDCICLSNGFCG 80
          TTDDDCMNDCICLSNGFCG
Sbjct: 61 TTDDDCMNDCICLSNGFCG 79

[Close]

BLAST of CSPI04G00300 vs. NCBI nr
Match: KGN52760.1 (hypothetical protein Csa_014741 [Cucumis sativus])

HSP 1 Score: 166.0 bits (419), Expect = 1.3e-37
Identity = 79/79 (100.00%), Postives = 79/79 (100.00%), Query Frame = 0


Query: 1  MEWKKIGMLAVVGMLVMAALVEDCHAAIESESVKGLNKNELPRKMMNEETRMCPRILEEC 60
          MEWKKIGMLAVVGMLVMAALVEDCHAAIESESVKGLNKNELPRKMMNEETRMCPRILEEC
Sbjct: 1  MEWKKIGMLAVVGMLVMAALVEDCHAAIESESVKGLNKNELPRKMMNEETRMCPRILEEC 60

Query: 61 TTDDDCMNDCICLSNGFCG 80
          TTDDDCMNDCICLSNGFCG
Sbjct: 61 TTDDDCMNDCICLSNGFCG 79

[Close]

BLAST of CSPI04G00300 vs. NCBI nr
Match: XP_022984819.1 (trypsin inhibitor 1-like [Cucurbita maxima])

HSP 1 Score: 73.2 bits (178), Expect = 1.2e-09
Identity = 41/82 (50.00%), Postives = 51/82 (62.20%), Query Frame = 0


Query: 1  MEWKKIGMLAVVGMLVMAALVEDCHAAIESESVKGL---NKNELPRKMMNEETRMCPRIL 60
          MEWK+  ++A+VG+L+MAA  E   AA+    V  L     N+ PRKMMN   + CPRIL
Sbjct: 1  MEWKRGALIAMVGLLLMAAFAES--AAVHGGEVIQLVSDGVNDFPRKMMN-GVQGCPRIL 60

Query: 61 EECTTDDDCMNDCICLSNGFCG 80
           EC  D DC+  CIC  NGFCG
Sbjct: 61 MECELDSDCLPGCICRPNGFCG 79

[Close]

BLAST of CSPI04G00300 vs. NCBI nr
Match: KAG6576915.1 (hypothetical protein SDJN03_24489, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 72.4 bits (176), Expect = 2.0e-09
Identity = 41/82 (50.00%), Postives = 50/82 (60.98%), Query Frame = 0


Query: 1   MEWKKIGMLAVVGMLVMAALVEDCHAAIESESVKGL---NKNELPRKMMNEETRMCPRIL 60
           MEWK+  ++A+VG+L+MAA  E   AA+    V  L     N+ PRKMMN     CPRIL
Sbjct: 83  MEWKRGALIAMVGLLLMAAFAES--AAVHGGEVIQLVSDGVNDFPRKMMNGALG-CPRIL 142

Query: 61  EECTTDDDCMNDCICLSNGFCG 80
            EC  D DC+  CIC  NGFCG
Sbjct: 143 MECEVDSDCLPGCICRPNGFCG 161

[Close]

BLAST of CSPI04G00300 vs. NCBI nr
Match: KAG7014942.1 (hypothetical protein SDJN02_22573, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 72.4 bits (176), Expect = 2.0e-09
Identity = 41/82 (50.00%), Postives = 50/82 (60.98%), Query Frame = 0


Query: 1   MEWKKIGMLAVVGMLVMAALVEDCHAAIESESVKGL---NKNELPRKMMNEETRMCPRIL 60
           MEWK+  ++A+VG+L+MAA  E   AA+    V  L     N+ PRKMMN     CPRIL
Sbjct: 83  MEWKRGALIAMVGLLLMAAFAES--AAVHGGEVIQLVSDGVNDFPRKMMNGALG-CPRIL 142

Query: 61  EECTTDDDCMNDCICLSNGFCG 80
            EC  D DC+  CIC  NGFCG
Sbjct: 143 MECEVDSDCLPGCICRPNGFCG 161

The following BLAST results are available for this feature:

BLAST of CSPI04G00300 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P34950	1.3e-08	64.29	Trypsin inhibitor 5 OS=Luffa aegyptiaca OX=3670 PE=1 SV=1	[more]
Q43667	7.4e-07	46.97	Trypsin inhibitor 1 OS=Trichosanthes kirilowii OX=3677 PE=3 SV=1	[more]
Q9S8I2	7.4e-07	68.97	Trypsin inhibitor 3 OS=Luffa aegyptiaca OX=3670 PE=1 SV=1	[more]
P25849	3.7e-06	65.52	Trypsin inhibitor 1 OS=Luffa aegyptiaca OX=3670 PE=1 SV=1	[more]
P32041	1.4e-05	62.07	Trypsin inhibitor 3 OS=Cucumis melo var. conomon OX=3657 PE=1 SV=1	[more]

BLAST of CSPI04G00300 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KT01	6.4e-38	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G000805 PE=3 SV=1	[more]
A0A6J1JBL8	5.7e-10	50.00	trypsin inhibitor 1-like OS=Cucurbita maxima OX=3661 GN=LOC111482991 PE=3 SV=1	[more]
A0A5D3E148	9.0e-08	40.22	Trypsin inhibitor 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol...	[more]
A0A0A0KY34	2.6e-07	38.27	Trypsin inhibitor 1 OS=Cucumis sativus OX=3659 GN=Csa_4G000810 PE=3 SV=1	[more]
A0A5D3E0W0	1.0e-06	40.74	Trypsin inhibitor 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G...	[more]

BLAST of CSPI04G00300 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_031739937.1	1.3e-37	100.00	uncharacterized protein LOC105435333 [Cucumis sativus]	[more]
KGN52760.1	1.3e-37	100.00	hypothetical protein Csa_014741 [Cucumis sativus]	[more]
XP_022984819.1	1.2e-09	50.00	trypsin inhibitor 1-like [Cucurbita maxima]	[more]
KAG6576915.1	2.0e-09	50.00	hypothetical protein SDJN03_24489, partial [Cucurbita argyrosperma subsp. sorori...	[more]
KAG7014942.1	2.0e-09	50.00	hypothetical protein SDJN02_22573, partial [Cucurbita argyrosperma subsp. argyro...	[more]

BLAST of CSPI04G00300 vs. NCBI nr


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SMART	SM00286	PTI_1	coord: 51..79 e-value: 6.6E-12 score: 55.6
None	No IPR available	GENE3D	4.10.75.20		coord: 31..79 e-value: 1.6E-15 score: 58.6
IPR000737	Proteinase inhibitor I7, squash	PFAM	PF00299	Squash	coord: 51..79 e-value: 9.8E-15 score: 54.3
IPR000737	Proteinase inhibitor I7, squash	PROSITE	PS00286	SQUASH_INHIBITOR	coord: 53..72
IPR000737	Proteinase inhibitor I7, squash	CDD	cd00150	PlantTI	coord: 53..79 e-value: 9.26201E-10 score: 46.9871
IPR011052	Proteinase/amylase inhibitor domain superfamily	SUPERFAMILY	57027	Plant inhibitors of proteinases and amylases	coord: 48..79

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI04G00300.1	CSPI04G00300.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity
molecular_function	GO:0004866	endopeptidase inhibitor activity