CSPI04G00050 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI04G00050
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionAspartate--tRNA ligase
LocationChr4: 32823 .. 41678 (-)
RNA-Seq ExpressionCSPI04G00050
SyntenyCSPI04G00050
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAGTCTAAGGCGTCTTCATCCACACGCAGTGCGTTCTACCCTCCATTTTCAGAATCCTTAAACCCTTCGCCTCCTTCAATCTTCCATTACACCCCATCTGAAAATCCTCCGCGCCATGTCCCGTTCTCTCCTCCGAGCTTTTCCTTTCTCCACTATTCGCCCAAAGCCTCCTCAATTTCTCCCCATCCGCCACCACATTTCTCATCTCCGCTTTCCCCAATCCATTTCCACAACTCACAGACCTGTCTCCTCATTCTCCACTTCCGCCACTCCTTCTCCTTCCTCCGCCACCGAAATCCTTGTCCCCACTTCGAATCCTCACTCCCCTTTTAAAGACTCTCTTCAGTGGATCAATCGTACGGCGCTCTGTGGTGAATTGTCAGTTGATGATGTTGGGAAGCGGGTTCGGCTCTGTGGTTGGGTTGCTCTGCATAGGGTTCATGGTGGTTTGACGTTTCTCAATCTTAGAGATCACAGTGGTATTGTTCAGGTGCTTATTTTTTTTATTCCTCTGTTTGAATTTTTAGGATTAGTTTGAAATTGAAGTTGTGCTATTGTTGACAATTGGTTTTTACTACTTGAATTAGGTGACTACGCTCCCCAACGAGTTTCCAGATGCTCATTCGACTATTAACGATTTGCGACTTGAGTATGTTGTTGCCGTGGAAGGAACTGTTCGGTCACGGCCTGGGGATTCGGTTAATGTAAAAATGAAAACTGGTTTGATAGAGGTATGTAAGAATTTCAATTTTGTTTTACTGTTCATCTAGCCAGGAGTTATGCTCAAGCCTTCCCCCTACGATCTACTTGGCCAACTCCCGAGTAAACAACAATTTTAAACAAGATTTTGAAGTTATCAAGTACACAATGTAAGCCTTGAACCTAGTATCTTGGAGGAATTTCCGAAAATCTTAAGCCTTTAGCCAAATAAATTAGTTAATTCTTACTAATCAATAATTTTGGGAGTTCTTGGTCACGGTTATTAAAAGCTTGAGATGGGTTAGTTGCGGGGGAAAGTGTTTAGTTCTTTTGGGATTTTCCGGTGTCTCCAAATAGATGCTACTTCTAGCATGGAACTTGCTGGCCGTGTGGTGTTTATCTCAACTTGAAAGCAAACTTGAAAGCACGAATTCCAAGGTTGTAGCAATCAGAGCCACTTGTTTCTGGAGAATTTTTGAAGGAGGCCATAGAGATTGGGTTTGTTGAATTTAGTTTTATGGTGCAAAATTAACAGCCGGATCTTCTATGATAAGTCATCTCCTGCTGATAATTTTATGGCATTTTTTTTTGTAATACTACTTATTGGTGCAAGTACTCCGCTTCAAGGATTAGAGTCTTTTTGTTATATGAACAATATCAAATAAAAGTAAAACCATAAAATTAGTAACCTCACATACAATAAAAATATGAGCCAACAAATTAAATTTTATCACTATAGTGCCTGAGTTTTGAGAGTAATTGTCTTTCAAGATGGAACAAATTACTTTTCTTCTAGAATCCACACCTTCCTCGTTCACAAGATCTAATGAACTCTACTTTGTGGAGTAAACCAAACTTCAAAAAATGTTTAAACGAGAGAAAATTTTTAGGAGAAAAGTGTTCATGGGAAAATGTAAGTTCAATTTGGAGAGGAGGCACATGCACATGTAAGTGGCCATTTGAATAGTCTTGTCACCTTTTCAAGTCGGTTAAAACTTAAAATCTAAAAAGATAACCATTCACGAAAGAATGCAGCTTATGCAATAAAAAAGATGCAACCCTAAATGAAGAGTCAAATGCCATTTTTTTTTCTTTCTAATTCAACACTTTTACTTTATTCACTTGTTAGTTTTAGATATTTTCCTCTGTAATCTGCTTATGGTGGTTGTAAGCCCTTTTATACATTTCATTCATCAATGAAATGAACATCAGTTTATTCCTAAAAATGTGGGGATTTAATTGACTTTTTGGGCACTTCATGGATTATTCACAATAGTTTGTTTGGTATTTATAATGTTTTTGTGATAAATTTCAATTGGAGTGACTTTTTATAAGTTCCCACGGGTGCGTTTCTCACAACTTCTCAGTCATGGAATGAAAAGTAATGTCTTCCACAAAATAAAGAGACACAGATGGGTTTTAGGCTAGTTGCTGTTCTATTGAAGTATAGTAGAAATTGGACCTTTTTCTTCACTATCTTAGTTCTCTTCTTCTAGATCATTCTAAGTTACCAATGAATCAACAACCATATTACAGTTGTACTTATTCAGTTATTGTTATGGGGTTAATTTCATGATATATTCAAGGTTGCTGCGGAGCATGTTCAGATATTAAATTCTGTTGGATCAAAGTTGCCCTTTCTAGTTACAACAGCTGATGATGCAAAGGATTCTGCTAAAGAGGAAATTCGTCTGAGGTGATTATTTGATATACTATCACTTCAGGCCTAGGCCTAGACCCATGTCTATTTTATTTATGAGTTGAGTATTTATAGGTATCGGTACCTTGATCTACGAAGGAAACAAATGAATTTTAACATAATGCTTCGTCATAAAGTGGTGAAGCTCCTTCGAAGATATTTAGAGGATGTACATGGTTTTGTGGAGGTACTCCCTGTTTATAACTTTTAACCGACTGTTTGCCTTTCTTGAAGTTTACTTGAATTATTTTCTGCATCAGAACTTCATATTATGCTCTTTGAATGGTCAGAAATTTTTTTTCTCTATTATTCAATTCTGAACTTTTGATGGTTATAAGGTAGATTGAGACGCCAATACTTTCTCGATCTACTCCGGAAGGCGCTCGAGATTATCTGGTGCCATCTAGACTTCAGGTAGTCTACATAGACTCAACCTCTTATTGATTCTGGTTTCTTTCAAGGCAGGTTGTTTTGGTCACTAGTATTTTCCAATCTAAAAACTTCAAATAATTTTCTTGAGAAATCTGAATGTGACGTCATTTTTAGTTTCTATTACTACCTCTGTTCTTTATTGCCTTTTAATTTCTCCAGTTGCCTAGTTGGTATACCTTGTCAATTCAGATTCACATTTAAGTGCTTATAGATCATAATTGTCAACCAAGGCGTTAAGATGGTGTAGTTAGACGCATTTTTTTTTTGGGGGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTGTCGATTTTTCTCAAGATTGTTTGTGAAATTTCTGGACATAGAATTGTAATACGTGTGCCTCTTCATTTGATTGCCTGTGTTTCTGAGGTGGCGACGTTTTTATGTAATTAATTGTCTTTTCCTCAACCAAAAAAAAAAAAAAAAGAGAACATAATTGTTGTTTTCCAACAAAGAATATAACTTATTGCGTTCTTCTGCAATAACATAGTTGTTCTTTTCTACAAAACCTGAAACACAAAATATATCTATGTTTGTTTACATTACTTTGTTATATGGATTGCATTTTTCCATGGCTTGCAGGTCTGATTCAATTTAAATGTATATTTTGAAGCCAGGAACATTTTTTGCTTTGCCTCAAAGTCCACAGCTGTTTAAGCAAATGTTGATGGTGTCTGGATTTGATAAATATTATCAAATAGCAAGGTACTGACAGTTATATCATCAAGCACCATTTCCATCATGTTTATCTCCTTGGCCAAGCGATTTGGATCTGATACACTCAAATTTCCTGCATCTGTGTGTAAATTCATTTTTTGCTTGCAAGTGGATATAATGACAAGTAGTTTTTTTTGTTGTCATGAAAACGAGGAGCTAAGTTCAATTTATTTCTATTGAGTTGTGAGAATCAGACATCATACTTACACCTTCGGTTTCGTATCACTTTCTTTGTAGGTGTTTTCGAGATGAAGACCTAAGAGCTGACAGACAGCCTGAGTTTACGCAACTTGATATGGAATTATCATTTACAACTTTGGAAGACATGCTGAGACTCAATGAAGATTTGATTAGAAAGGTAGATTCTGATTATTAGAATTGACTTGATGATTGAATGTCACAATGCATATGCCTATGTATCCATTAATATTGGTTGCACTGTAAAACTATTTGAAAAGAAGCAAGGGTAATTGCCACAAACTTTTGCTCTCACGACCAACTTTTTGACGCATTCATTTTGTTGGCACCCATTTTATGCTTATGTGGGTTGCTGCCTGGTTTTGGGATTTTATGTTGGAGGGATCCAATTTTTCACAAACTAGTCAACTTTGTTGACAGCTCTTAGGTTCTTTGTATTAACTTTGTCTATAAGAGGTAGTAACTACATCTAAGAAGAGGAGGTTCTAAGGTCCAAGGCCTAAAAATAACACTTTTTGCTGTATTCATCCTTTGCCCTCCAATTTTTGACTACTGATCCTAAAGAAAAATTATATTCTTGTAGGTTTTCTGGGAAGTAAAAGGTGTTCAACTGCCGAATCCTTTCCCAAGGCTTACCTATGCTGAAGCAATGAGTCGATATGGATCAGATAGGCCGGATACAAGATTTGATCTTCAATTGAATGATGTAAACTTTTCTCCTTTCTATATTTGGATCCAACTCTTTCTGGATTAAATTTCCAAATAAATTTATTTTTATTTTATTTTATTTTAAAATGTAATCATTGTCTTGAACCAATGACTGTGAATTGGAATAAGGAACCTTCTGGCTTTTACTGTGCTTTAAAGCTGTTTCCTATTTAGAAAATGTTCAGTCGTTTATGTGTTGAATCTTGTTCTTTGGCGTTTTTCTACTGTTTCATGTTTATATAAGTCTGGATGACTTTAGGCTTTAGGGTACTGGCAGTATTCTTTATAACAAAGATATTTTTATAGCTTTCGACACTCCTACTGAAGTGTTCGTTCATCATATCTAGTATATATGATTAGATGATAAAGAAGGGATCTTGGAATATGATCAACTACTCACATGTTCACAGATTTTGAAAACTGTTGGACTTATCACACCATGTTTATAATCGACTGTTTTCAGTTTATTGAAGCCCAGTTTCCAATTCAAATTAGGTGATGATATTGTTCACGGGAGTTAAGTACTTTTTATATGAATTAAAGATTCCCTCTGAATTAGCTATTGATAGACTTTCTCTTTGGAGCTGCAGGTATCTGATATTTTTGCAGATTCTCCATTTAAGGTTTTTGCAGACGCCTTGTCTAGTGGTGGAATCATCAAAGTACTGTGTGTGCCATCTGGTGCTAAAAGGTACTCAAATACAGCTCTTAAAAAGGGTGACATTTATACAGAGGCGACCAAATCTGGTGCAAAAGGATTACCTTTTCTGAAAGTTACAGACGATGGTACTATTCCTTCTCTCCTCAAGATTTCCAATTTGCTTAAAATGTCAATTGTTGAGATGGTGTTTGGGTTGTTCTTGGGGGATATCTTTCAGGTGGCATTGAAGGGATTCCTGCCCTAGTATCATGTTTGGATCCTACAGATAGAGAGGAATTTCTAAATAGATGCTCTGCCGGACCTGGTGATCTAATCTTGTTTGCCGTGGGCCATCGAGTATCAGTTAATAAAACTCTGGATAGGCTTAGACTATATGTAGCTAATGAGCTGGGTTTAGTCGACCACGTAAGTTTATGTTTTCTATATTTTGGTAGTTTTATGCTAAATGTTCATTTTCGTTCTCACTTATTATCGTTTTCTGTCTATTTAGTCTAGGCATTCAATTTTGTGGGTGACTGATTTTCCAATGTTTGAATGGAATGATCTAGATCAACGACTCGAGGTTTGACTTGTATATTTATAATGTCTCTTGTGTAGCCTCTTATTTTGCACCCTAGTCTGCACTTCAGGCTAAAACATAGTTTGTTTTATTCTTTGGCTTTTAGTTGTTTATAAATGCAATTATGTTTTGCAGGCTTTGCACCATCCTTTCACAGCTCCAAACCCTGAAGACGTTGGTGATCTGCCCTCTGCCCGTGCTTTAGCTTATGACATGGTTTACAATGGAGTAGAGGTAGTTACTATTGCTCAATTTTTTTTTGAACTCATAGTAATAAAAAAAGAGAAATCATACTTCCATTGGAATGAAAAAAACACATAGAATGACCAAAATAATAAAAAACAAAAAACAAAAACCTAACTATTCAAAAAGAACTTGAATTCTTGAGAATAAGGTTGAAATACTTTGTGTCGCAAATTTGGAAGAGCTGCTACTATTTGTCTTTACTACAGCTTACACACTTGAGTTAAGTGCGCTCTTCTTGAGTTAAAATTATCTTCACTACGTTTGTTGCTGTAGATTGGTGGAGGCAGTTTGAGAATTCATAAACGTGAAGTTCAAGAAAAGGTTTTGGAGATTGTGGGAATCTCTCTTGAACAGGTAAAAGATGAAGGGTTGCATGATTCTCTATAATATACTTGAGAATTAATTGAATGCACACACAAGAAACGGGATCAATCTGTTGGACCAAATCTTGTAGGTTCTTTTTGGTTCTCGGTCTAACCGAACCGACCACGTTCTGTTTGATTGGTGGCTTGATTATGTTACAACACAACTGATATAATTATTGATGTTAATTTTAATTCTATGAATTTATACAATTTGGTTTGGTCCCCCCTCAATCTGGTTTACCCCCATTAATCTATGAGGCTGAATCGAAGGTTCAGCTGGTCCAGCCAAGATATTATCTCCTACAATCAATTTGGTTTCTTCCTAATTCCTAATTTCGATGTGAAATGAAAATCAATGCTATTTTCCAGCAACGTGATTTGGGTTTTTTTTCTTTTTGCCGACAATATGAATTTTGTTCTTTTATGGCTTGGCAGGCAGAAGCGAAGTTTGGTTATTTATTGGAGGCACTAGACATGGGTGCCCCGCCTCACGGTATGGACCATTAAAGCTCTCTTCCAGTTTCTAAACTTAAAAGCCACCCTTATTGTTTGGACGATTTAACTAATATCTTATCACTGTTCCTATCAATGATCAAATGCCTCGCGTAGGTAGGGAAAATGCATTTTCATCATGCAACCACTAATAATATATTGAAAATCTTAATAATCTAACCTATGTATGCTAACATAGGCTTTTAAAAGTGATTTGCCTATTTTCTTACTGAATCTGGTTGTTGAATTTTTTGTATAATTCTTTAAGGGGAAACTTGAAAACTTATATTTGGTCTTTGCACCCGATCTGACATGTGGAAGAATAGCGTGAGGAAGAACTTTCTATTCAAGTGATTATAGGGAACTTAAATTTTCCACCTATGTTGTAGTTTTCAATATTTATTCAATCTCTAGGATTGGAAAATCAGCCGGCGGTGGCCTTTGGGTAAGACTTGAAACAATCACCCTATCTTTGAAGACCCCATGTAGAAACTTTAATAATCTTAATTGATTGTTTGTCTCCTGAAGACAGTTTCAGGTGAAGTTTCATAAAAAATTAGTGGGTCATGAGTTTAATTCCTTGGGTACTTTGGATATCCAAAAGAAGAGGGAAATAGGAAAAAGAACTCCAACCATCATGGGTTGGCTTAGTGCTAAAAAAAGTAGACAATCTCAATAACTAAGAGGCACCAACCTAGGAACCGATTTCCTGTGAGTTTCCTTGGCACCCAAATAATGTAGGGTTTGGTGGGTTATCCCATAAGATTGTCGAGGTATACATAATCCAGACACTCACGGGTATAAAAAAAAAGTAAACAAAAGAAAAAAATCCAATGCGAGGGGTAGCTGTAGGTGTGGACTGACTAATAGATGGGTGATGTATGTTATCGGCAAGGATTGGGAATTGAAATATCAACTTTTGGAGTGGTCATTAGTCTTTATCCATTAAACTTTGCTGAAATATCTACTTTTGGAATGGTCATTGATGTCTTTATCCATTAAACTATGCTGAGATTGACTTATATTTCAAATATGTGACTCAACAAACATTATTATTATTATTAGTATTATTTGTTTTTACTCTTCTTTTCTGTTTGTGTAGGGGGCATTGCTTATGGATTGGACAGATTAGTCATGTTATTAGCAGATGCAAATTCCATTAGGGATGTGATAGCTTTCCCAAAGACAACAACAGCACAATGTGCCCTCACAAGAGCTCCTTCAGAAGTGGATCCCCAACAACTCAAGGATTTATCATATAAGAGCCAATAAAAAATTCATTCAAATACTCCTTTGGTTTCGTTCATACTCAACTTTTAATTTTATATTCACCTCCTCCTCACTCGAGCAGCAGTTTTTCAGCTCGAGAAAAATGGACAACGGCCTCATTGCATATTCAACTTTATAATTTAACAAAAAAATTCAAATTCTCCGGTTTCAACATCAAACAGAGATCAGATTTTGGAAAGATATCGTTTTGTGAGTTCAACCTAATGTTTAGGTAAGGCTGAGGATGGATGTGTTGCAGTTTGTTGGTGTCATGTAGAGAAGAGGTGAAAGTGTGTGTAATACAATTACATACATAGTGTGGACAGAGTGTATTGTGTTTGGGAGTTTGTTAATTAAGTCTTTTAAATTTGGTTTGGACATTTCTGGTGTTTATGAATTTGGTGCAGACAGAGTATTATCAGTTGTCAAACATTCAAATGTAGAATCATGTGGCCAATTAGATATGGGTATCCCACATTGCTTGGATTCTCTCTACCATAAAGCTGTAGACACAATAAAGGTATCTATAAGGATATAATAGTAAGAACTTTGTTCTCTTACTCTTACAAGTTTTATTCTTCACTTCT

mRNA sequence

AAGTCTAAGGCGTCTTCATCCACACGCAGTGCGTTCTACCCTCCATTTTCAGAATCCTTAAACCCTTCGCCTCCTTCAATCTTCCATTACACCCCATCTGAAAATCCTCCGCGCCATGTCCCGTTCTCTCCTCCGAGCTTTTCCTTTCTCCACTATTCGCCCAAAGCCTCCTCAATTTCTCCCCATCCGCCACCACATTTCTCATCTCCGCTTTCCCCAATCCATTTCCACAACTCACAGACCTGTCTCCTCATTCTCCACTTCCGCCACTCCTTCTCCTTCCTCCGCCACCGAAATCCTTGTCCCCACTTCGAATCCTCACTCCCCTTTTAAAGACTCTCTTCAGTGGATCAATCGTACGGCGCTCTGTGGTGAATTGTCAGTTGATGATGTTGGGAAGCGGGTTCGGCTCTGTGGTTGGGTTGCTCTGCATAGGGTTCATGGTGGTTTGACGTTTCTCAATCTTAGAGATCACAGTGGTATTGTTCAGGTGACTACGCTCCCCAACGAGTTTCCAGATGCTCATTCGACTATTAACGATTTGCGACTTGAGTATGTTGTTGCCGTGGAAGGAACTGTTCGGTCACGGCCTGGGGATTCGGTTAATGTAAAAATGAAAACTGGTTTGATAGAGGTTGCTGCGGAGCATGTTCAGATATTAAATTCTGTTGGATCAAAGTTGCCCTTTCTAGTTACAACAGCTGATGATGCAAAGGATTCTGCTAAAGAGGAAATTCGTCTGAGGTATCGGTACCTTGATCTACGAAGGAAACAAATGAATTTTAACATAATGCTTCGTCATAAAGTGGTGAAGCTCCTTCGAAGATATTTAGAGGATGTACATGGTTTTGTGGAGATTGAGACGCCAATACTTTCTCGATCTACTCCGGAAGGCGCTCGAGATTATCTGGTGCCATCTAGACTTCAGCCAGGAACATTTTTTGCTTTGCCTCAAAGTCCACAGCTGTTTAAGCAAATGTTGATGGTGTCTGGATTTGATAAATATTATCAAATAGCAAGGTGTTTTCGAGATGAAGACCTAAGAGCTGACAGACAGCCTGAGTTTACGCAACTTGATATGGAATTATCATTTACAACTTTGGAAGACATGCTGAGACTCAATGAAGATTTGATTAGAAAGGTTTTCTGGGAAGTAAAAGGTGTTCAACTGCCGAATCCTTTCCCAAGGCTTACCTATGCTGAAGCAATGAGTCGATATGGATCAGATAGGCCGGATACAAGATTTGATCTTCAATTGAATGATGTATCTGATATTTTTGCAGATTCTCCATTTAAGGTTTTTGCAGACGCCTTGTCTAGTGGTGGAATCATCAAAGTACTGTGTGTGCCATCTGGTGCTAAAAGGTACTCAAATACAGCTCTTAAAAAGGGTGACATTTATACAGAGGCGACCAAATCTGGTGCAAAAGGATTACCTTTTCTGAAAGTTACAGACGATGGTGGCATTGAAGGGATTCCTGCCCTAGTATCATGTTTGGATCCTACAGATAGAGAGGAATTTCTAAATAGATGCTCTGCCGGACCTGGTGATCTAATCTTGTTTGCCGTGGGCCATCGAGTATCAGTTAATAAAACTCTGGATAGGCTTAGACTATATGTAGCTAATGAGCTGGGTTTAGTCGACCACTCTAGGCATTCAATTTTGTGGGTGACTGATTTTCCAATGTTTGAATGGAATGATCTAGATCAACGACTCGAGGCTTTGCACCATCCTTTCACAGCTCCAAACCCTGAAGACGTTGGTGATCTGCCCTCTGCCCGTGCTTTAGCTTATGACATGGTTTACAATGGAGTAGAGATTGGTGGAGGCAGTTTGAGAATTCATAAACGTGAAGTTCAAGAAAAGGTTTTGGAGATTGTGGGAATCTCTCTTGAACAGGCAGAAGCGAAGTTTGGTTATTTATTGGAGGCACTAGACATGGGTGCCCCGCCTCACGGGGGCATTGCTTATGGATTGGACAGATTAGTCATGTTATTAGCAGATGCAAATTCCATTAGGGATGTGATAGCTTTCCCAAAGACAACAACAGCACAATGTGCCCTCACAAGAGCTCCTTCAGAAGTGGATCCCCAACAACTCAAGGATTTATCATATAAGAGCCAATAAAAAATTCATTCAAATACTCCTTTGGTTTCGTTCATACTCAACTTTTAATTTTATATTCACCTCCTCCTCACTCGAGCAGCAGTTTTTCAGCTCGAGAAAAATGGACAACGGCCTCATTGCATATTCAACTTTATAATTTAACAAAAAAATTCAAATTCTCCGGTTTCAACATCAAACAGAGATCAGATTTTGGAAAGATATCGTTTTGTGAGTTCAACCTAATGTTTAGGTAAGGCTGAGGATGGATGTGTTGCAGTTTGTTGGTGTCATGTAGAGAAGAGGTGAAAGTGTGTGTAATACAATTACATACATAGTGTGGACAGAGTGTATTGTGTTTGGGAGTTTGTTAATTAAGTCTTTTAAATTTGGTTTGGACATTTCTGGTGTTTATGAATTTGGTGCAGACAGAGTATTATCAGTTGTCAAACATTCAAATGTAGAATCATGTGGCCAATTAGATATGGGTATCCCACATTGCTTGGATTCTCTCTACCATAAAGCTGTAGACACAATAAAGGTATCTATAAGGATATAATAGTAAGAACTTTGTTCTCTTACTCTTACAAGTTTTATTCTTCACTTCT

Coding sequence (CDS)

ATGTCCCGTTCTCTCCTCCGAGCTTTTCCTTTCTCCACTATTCGCCCAAAGCCTCCTCAATTTCTCCCCATCCGCCACCACATTTCTCATCTCCGCTTTCCCCAATCCATTTCCACAACTCACAGACCTGTCTCCTCATTCTCCACTTCCGCCACTCCTTCTCCTTCCTCCGCCACCGAAATCCTTGTCCCCACTTCGAATCCTCACTCCCCTTTTAAAGACTCTCTTCAGTGGATCAATCGTACGGCGCTCTGTGGTGAATTGTCAGTTGATGATGTTGGGAAGCGGGTTCGGCTCTGTGGTTGGGTTGCTCTGCATAGGGTTCATGGTGGTTTGACGTTTCTCAATCTTAGAGATCACAGTGGTATTGTTCAGGTGACTACGCTCCCCAACGAGTTTCCAGATGCTCATTCGACTATTAACGATTTGCGACTTGAGTATGTTGTTGCCGTGGAAGGAACTGTTCGGTCACGGCCTGGGGATTCGGTTAATGTAAAAATGAAAACTGGTTTGATAGAGGTTGCTGCGGAGCATGTTCAGATATTAAATTCTGTTGGATCAAAGTTGCCCTTTCTAGTTACAACAGCTGATGATGCAAAGGATTCTGCTAAAGAGGAAATTCGTCTGAGGTATCGGTACCTTGATCTACGAAGGAAACAAATGAATTTTAACATAATGCTTCGTCATAAAGTGGTGAAGCTCCTTCGAAGATATTTAGAGGATGTACATGGTTTTGTGGAGATTGAGACGCCAATACTTTCTCGATCTACTCCGGAAGGCGCTCGAGATTATCTGGTGCCATCTAGACTTCAGCCAGGAACATTTTTTGCTTTGCCTCAAAGTCCACAGCTGTTTAAGCAAATGTTGATGGTGTCTGGATTTGATAAATATTATCAAATAGCAAGGTGTTTTCGAGATGAAGACCTAAGAGCTGACAGACAGCCTGAGTTTACGCAACTTGATATGGAATTATCATTTACAACTTTGGAAGACATGCTGAGACTCAATGAAGATTTGATTAGAAAGGTTTTCTGGGAAGTAAAAGGTGTTCAACTGCCGAATCCTTTCCCAAGGCTTACCTATGCTGAAGCAATGAGTCGATATGGATCAGATAGGCCGGATACAAGATTTGATCTTCAATTGAATGATGTATCTGATATTTTTGCAGATTCTCCATTTAAGGTTTTTGCAGACGCCTTGTCTAGTGGTGGAATCATCAAAGTACTGTGTGTGCCATCTGGTGCTAAAAGGTACTCAAATACAGCTCTTAAAAAGGGTGACATTTATACAGAGGCGACCAAATCTGGTGCAAAAGGATTACCTTTTCTGAAAGTTACAGACGATGGTGGCATTGAAGGGATTCCTGCCCTAGTATCATGTTTGGATCCTACAGATAGAGAGGAATTTCTAAATAGATGCTCTGCCGGACCTGGTGATCTAATCTTGTTTGCCGTGGGCCATCGAGTATCAGTTAATAAAACTCTGGATAGGCTTAGACTATATGTAGCTAATGAGCTGGGTTTAGTCGACCACTCTAGGCATTCAATTTTGTGGGTGACTGATTTTCCAATGTTTGAATGGAATGATCTAGATCAACGACTCGAGGCTTTGCACCATCCTTTCACAGCTCCAAACCCTGAAGACGTTGGTGATCTGCCCTCTGCCCGTGCTTTAGCTTATGACATGGTTTACAATGGAGTAGAGATTGGTGGAGGCAGTTTGAGAATTCATAAACGTGAAGTTCAAGAAAAGGTTTTGGAGATTGTGGGAATCTCTCTTGAACAGGCAGAAGCGAAGTTTGGTTATTTATTGGAGGCACTAGACATGGGTGCCCCGCCTCACGGGGGCATTGCTTATGGATTGGACAGATTAGTCATGTTATTAGCAGATGCAAATTCCATTAGGGATGTGATAGCTTTCCCAAAGACAACAACAGCACAATGTGCCCTCACAAGAGCTCCTTCAGAAGTGGATCCCCAACAACTCAAGGATTTATCATATAAGAGCCAATAA

Protein sequence

MSRSLLRAFPFSTIRPKPPQFLPIRHHISHLRFPQSISTTHRPVSSFSTSATPSPSSATEILVPTSNPHSPFKDSLQWINRTALCGELSVDDVGKRVRLCGWVALHRVHGGLTFLNLRDHSGIVQVTTLPNEFPDAHSTINDLRLEYVVAVEGTVRSRPGDSVNVKMKTGLIEVAAEHVQILNSVGSKLPFLVTTADDAKDSAKEEIRLRYRYLDLRRKQMNFNIMLRHKVVKLLRRYLEDVHGFVEIETPILSRSTPEGARDYLVPSRLQPGTFFALPQSPQLFKQMLMVSGFDKYYQIARCFRDEDLRADRQPEFTQLDMELSFTTLEDMLRLNEDLIRKVFWEVKGVQLPNPFPRLTYAEAMSRYGSDRPDTRFDLQLNDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSNTALKKGDIYTEATKSGAKGLPFLKVTDDGGIEGIPALVSCLDPTDREEFLNRCSAGPGDLILFAVGHRVSVNKTLDRLRLYVANELGLVDHSRHSILWVTDFPMFEWNDLDQRLEALHHPFTAPNPEDVGDLPSARALAYDMVYNGVEIGGGSLRIHKREVQEKVLEIVGISLEQAEAKFGYLLEALDMGAPPHGGIAYGLDRLVMLLADANSIRDVIAFPKTTTAQCALTRAPSEVDPQQLKDLSYKSQ*
Homology
BLAST of CSPI04G00050 vs. ExPASy Swiss-Prot
Match: F4JJT9 (Aspartate--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At4g33760 PE=2 SV=1)

HSP 1 Score: 979.9 bits (2532), Expect = 1.4e-284
Identity = 490/667 (73.46%), Postives = 567/667 (85.01%), Query Frame = 0

Query: 5   LLRAFPFSTIRPKPPQFLPIRHHISHLRFPQSISTTHRPVSSFSTSATPSPSSATEILV- 64
           LLR  P    R      + I +  +    P+   T   P +  + S++P  +++ +++V 
Sbjct: 4   LLRTLPLRPTRFLSATAISISNATNFFVVPK--RTNPLPGTRRTFSSSPVAAASGDVVVK 63

Query: 65  PTSNPHSPFKDSLQWINRTALCGELSVDDVGKRVRLCGWVALHRVHGGLTFLNLRDHSGI 124
           P  +P S     L+W++RT LCGELSV+DVGKRV LCGWVALHRVHGGLTFLNLRDH+GI
Sbjct: 64  PVPSPPS----VLRWVSRTELCGELSVNDVGKRVHLCGWVALHRVHGGLTFLNLRDHTGI 123

Query: 125 VQVTTLPNEFPDAHSTINDLRLEYVVAVEGTVRSRPGDSVNVKMKTGLIEVAAEHVQILN 184
           VQV TLP+EFP+AH  IND+RLEYVV VEGTVRSRP +SVN KMKTG +EV AEHV+ILN
Sbjct: 124 VQVRTLPDEFPEAHGLINDMRLEYVVLVEGTVRSRPNESVNKKMKTGFVEVVAEHVEILN 183

Query: 185 SVGSKLPFLVTTADDAKDSAKEEIRLRYRYLDLRRKQMNFNIMLRHKVVKLLRRYLEDVH 244
            V +KLPFLVTTAD+ KD  KEEIRLR+R LDLRR+QM  NI+LRH VVKL+RRYLED H
Sbjct: 184 PVRTKLPFLVTTADENKDLIKEEIRLRFRCLDLRRQQMKNNIVLRHNVVKLIRRYLEDRH 243

Query: 245 GFVEIETPILSRSTPEGARDYLVPSRLQPGTFFALPQSPQLFKQMLMVSGFDKYYQIARC 304
           GF+EIETPILSRSTPEGARDYLVPSR+Q GTF+ALPQSPQLFKQMLMVSGFDKYYQIARC
Sbjct: 244 GFIEIETPILSRSTPEGARDYLVPSRIQSGTFYALPQSPQLFKQMLMVSGFDKYYQIARC 303

Query: 305 FRDEDLRADRQPEFTQLDMELSFTTLEDMLRLNEDLIRKVFWEVKGVQLPNPFPRLTYAE 364
           FRDEDLRADRQPEFTQLDME++F  +EDML+LNEDLIRKVF E+KG+QLP+PFPRLTYA+
Sbjct: 304 FRDEDLRADRQPEFTQLDMEMAFMPMEDMLKLNEDLIRKVFSEIKGIQLPDPFPRLTYAD 363

Query: 365 AMSRYGSDRPDTRFDLQLNDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSNTAL 424
           AM RYGSDRPDTRFDL+L DVS++F +S F+VF +AL SGGIIKVLCVP GAK+YSN+AL
Sbjct: 364 AMDRYGSDRPDTRFDLELKDVSNVFTESSFRVFTEALESGGIIKVLCVPLGAKKYSNSAL 423

Query: 425 KKGDIYTEATKSGAKGLPFLKVTDDGGIEGIPALVSCLDPTDREEFLNRCSAGPGDLILF 484
           KKGDIY EA KSGAKGLPFLKV D+G IEGI ALVS LD   +  F+ +C A PGDLILF
Sbjct: 424 KKGDIYNEAMKSGAKGLPFLKVLDNGEIEGIAALVSSLDSAGKINFVKQCGAAPGDLILF 483

Query: 485 AVGHRVSVNKTLDRLRLYVANELGLVDHSRHSILWVTDFPMFEWNDLDQRLEALHHPFTA 544
            VG   SVNKTLDRLRL+VA+++ L+DHS+HSILWVTDFPMFEWN+ +QRLEALHHPFTA
Sbjct: 484 GVGPVTSVNKTLDRLRLFVAHDMDLIDHSKHSILWVTDFPMFEWNEPEQRLEALHHPFTA 543

Query: 545 PNPEDVGDLPSARALAYDMVYNGVEIGGGSLRIHKREVQEKVLEIVGISLEQAEAKFGYL 604
           P PED+ DLPSARALAYDMVYNGVEIGGGSLRI+KR+VQEKVLEI+GIS E+AE+KFGYL
Sbjct: 544 PKPEDMDDLPSARALAYDMVYNGVEIGGGSLRIYKRDVQEKVLEIIGISPEEAESKFGYL 603

Query: 605 LEALDMGAPPHGGIAYGLDRLVMLLADANSIRDVIAFPKTTTAQCALTRAPSEVDPQQLK 664
           LEALDMGAPPHGGIAYGLDR+VM+L  A+SIRDVIAFPKTTTAQCALTR PSEVDP+QL+
Sbjct: 604 LEALDMGAPPHGGIAYGLDRMVMMLGGASSIRDVIAFPKTTTAQCALTRTPSEVDPKQLQ 663

Query: 665 DLSYKSQ 671
           DLS +++
Sbjct: 664 DLSIRTK 664

BLAST of CSPI04G00050 vs. ExPASy Swiss-Prot
Match: Q112K0 (Aspartate--tRNA(Asp/Asn) ligase OS=Trichodesmium erythraeum (strain IMS101) OX=203124 GN=aspS PE=3 SV=1)

HSP 1 Score: 734.6 bits (1895), Expect = 1.0e-210
Identity = 353/590 (59.83%), Postives = 459/590 (77.80%), Query Frame = 0

Query: 81  RTALCGELSVDDVGKRVRLCGWVALHRVHGGLTFLNLRDHSGIVQVTTLPNEFPDAHSTI 140
           RT  CG+++ +++ + V LCGWV   R HGG+ FL+LRD SG++Q+ + P   PD++   
Sbjct: 2   RTHYCGQVNSNNIEETVNLCGWVDRRRDHGGVIFLDLRDRSGLIQIVSDPERTPDSYQIA 61

Query: 141 NDLRLEYVVAVEGTVRSRPGDSVNVKMKTGLIEVAAEHVQILNSVGSKLPFLVTTADDAK 200
            D+R EYVV + G V  RP +S+N K+ TG IE+ A+ +Q+LN +G +LPF V+TA+   
Sbjct: 62  GDIRNEYVVQITGRVSRRPEESLNPKLPTGEIEIYADDIQLLNGLGKQLPFQVSTAE--T 121

Query: 201 DSAKEEIRLRYRYLDLRRKQMNFNIMLRHKVVKLLRRYLEDVHGFVEIETPILSRSTPEG 260
           ++ +EE+RL+YRYLDLRR++M  N++LRH+V+K +RR+LED   FVEIETPIL+RSTPEG
Sbjct: 122 EAVREELRLKYRYLDLRRERMTRNLLLRHEVIKAIRRFLEDEQNFVEIETPILTRSTPEG 181

Query: 261 ARDYLVPSRLQPGTFFALPQSPQLFKQMLMVSGFDKYYQIARCFRDEDLRADRQPEFTQL 320
           ARDYLVPSR+ PG +FALPQSPQLFKQ+LMVSGFD+YYQIARCFRDEDLRADRQPEFTQL
Sbjct: 182 ARDYLVPSRVHPGEWFALPQSPQLFKQILMVSGFDRYYQIARCFRDEDLRADRQPEFTQL 241

Query: 321 DMELSFTTLEDMLRLNEDLIRKVFWEVKGVQLPNPFPRLTYAEAMSRYGSDRPDTRFDLQ 380
           DME+SF + E++L+LNE L+  +F +VKG+ LP PFPRLTY EAM RYGSD+PD RF L+
Sbjct: 242 DMEMSFMSQEEILQLNEKLVAYIFQKVKGIDLPLPFPRLTYTEAMERYGSDKPDVRFGLE 301

Query: 381 LNDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSNTALKK-GDIYTEATKSGAKG 440
           L DVSD+  DS FKVF+ A++ GGI+KVL +P G    SN  +K  GD++ EA++ GAKG
Sbjct: 302 LVDVSDLMKDSGFKVFSGAIAKGGIVKVLPIPGGNDAISNVRIKPGGDLFKEASEVGAKG 361

Query: 441 LPFLKVTDDGGIEGIPALVSCLDPTDREEFLNRCSAGPGDLILFAVGHRVSVNKTLDRLR 500
           L +++V + G I+ I A+   L    ++E L R +A PG L+LFA     +VNKTLDRLR
Sbjct: 362 LAYVRVKEGGKIDTIGAIKDNLTDEQKQELLKRTNAQPGHLLLFAADDANTVNKTLDRLR 421

Query: 501 LYVANELGLVDHSRHSILWVTDFPMFEWNDLDQRLEALHHPFTAPNPEDVGDLPSARALA 560
           L +  +LGL+D  + S+LWVTDFPMFEWN+ +QRLEALHHPFTAP+P+D+ DL +ARA A
Sbjct: 422 LVIGEQLGLIDQDKISLLWVTDFPMFEWNEDEQRLEALHHPFTAPHPDDINDLKTARAQA 481

Query: 561 YDMVYNGVEIGGGSLRIHKREVQEKVLEIVGISLEQAEAKFGYLLEALDMGAPPHGGIAY 620
           YD+V+NG E+GGGSLRI++R+VQEKV E +G+S ++A+ KFG+LLEA + G PPHGGIAY
Sbjct: 482 YDLVFNGYEVGGGSLRIYQRDVQEKVFEAIGLSAQEADNKFGFLLEAFEYGTPPHGGIAY 541

Query: 621 GLDRLVMLLADANSIRDVIAFPKTTTAQCALTRAPSEVDPQQLKDLSYKS 670
           GLDRLVMLLA   SIRDVIAFPKT  A+C L  APSEVD +QLK+L   S
Sbjct: 542 GLDRLVMLLAVEESIRDVIAFPKTQQAKCLLANAPSEVDKKQLKELHVSS 589

BLAST of CSPI04G00050 vs. ExPASy Swiss-Prot
Match: Q8YUB6 (Aspartate--tRNA(Asp/Asn) ligase OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) OX=103690 GN=aspS PE=3 SV=1)

HSP 1 Score: 731.5 bits (1887), Expect = 8.7e-210
Identity = 356/590 (60.34%), Postives = 457/590 (77.46%), Query Frame = 0

Query: 81  RTALCGELSVDDVGKRVRLCGWVALHRVHGGLTFLNLRDHSGIVQVTTLPNEFPDAHSTI 140
           RT  CGEL   D+G+ V L GWV   R HGG+ FL+LRD SGIVQV + P   PD++   
Sbjct: 2   RTHYCGELRQKDIGETVTLYGWVDRRRDHGGVIFLDLRDRSGIVQVVSDPQRTPDSYELA 61

Query: 141 NDLRLEYVVAVEGTVRSRPGDSVNVKMKTGLIEVAAEHVQILNSVGSKLPFLVTTADDAK 200
           N LR EYVV + G V  RP +S+N ++ TG +E+ A+ +++LN V  +LPF V+TAD   
Sbjct: 62  NSLRNEYVVEITGRVTQRPEESLNTRIPTGEVEIYADKIELLNGVRKQLPFQVSTAD--T 121

Query: 201 DSAKEEIRLRYRYLDLRRKQMNFNIMLRHKVVKLLRRYLEDVHGFVEIETPILSRSTPEG 260
           ++ +E++RL+YRYLDLRR +M  NI LRH+VVK +RRYLEDV GF+E+ETPIL+RSTPEG
Sbjct: 122 ETVREDLRLKYRYLDLRRDRMARNIQLRHQVVKAMRRYLEDVEGFIEVETPILTRSTPEG 181

Query: 261 ARDYLVPSRLQPGTFFALPQSPQLFKQMLMVSGFDKYYQIARCFRDEDLRADRQPEFTQL 320
           ARDY++PSR+ PG +FALPQSPQLFKQ+LMVSG D+YYQIARCFRDEDLRADRQPEFTQL
Sbjct: 182 ARDYVLPSRVNPGEWFALPQSPQLFKQILMVSGLDRYYQIARCFRDEDLRADRQPEFTQL 241

Query: 321 DMELSFTTLEDMLRLNEDLIRKVFWEVKGVQLPNPFPRLTYAEAMSRYGSDRPDTRFDLQ 380
           DME+SF + E+++ LNE L+  +F  VKGV+LP PFPRLTYAEAM RYG D+PDTR+DLQ
Sbjct: 242 DMEMSFMSEEEIIELNEKLVSYIFKTVKGVELPLPFPRLTYAEAMERYGCDKPDTRYDLQ 301

Query: 381 LNDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSNTALKK-GDIYTEATKSGAKG 440
           L +VSD+  DS FKVF DA+++GGI+K+L +P+G ++ SN  +K  GD++ EA+++GAKG
Sbjct: 302 LVNVSDVMKDSGFKVFRDAVANGGIVKILPIPNGNEQISNVRIKPGGDLFREASEAGAKG 361

Query: 441 LPFLKVTDDGGIEGIPALVSCLDPTDREEFLNRCSAGPGDLILFAVGHRVSVNKTLDRLR 500
           L +++V +DG I+ I A+   L    ++E L R  A PG L+LF  G  V+VNKTLDRLR
Sbjct: 362 LAYIRVREDGEIDTIGAIKDNLSEEQKQEILQRTGAKPGHLLLFGAGDAVTVNKTLDRLR 421

Query: 501 LYVANELGLVDHSRHSILWVTDFPMFEWNDLDQRLEALHHPFTAPNPEDVGDLPSARALA 560
             +A E GL+D  + ++LWV DFPMFEWN  ++RLEALHHPFTAP+P+D+ DL +ARA A
Sbjct: 422 QAIAKEFGLIDPDKINLLWVVDFPMFEWNADEKRLEALHHPFTAPHPDDLHDLKTARAQA 481

Query: 561 YDMVYNGVEIGGGSLRIHKREVQEKVLEIVGISLEQAEAKFGYLLEALDMGAPPHGGIAY 620
           YD+V+NG E+GGGS RI++REVQE+V E +G+S E+A+ KFG+LLEA + G PPHGGIAY
Sbjct: 482 YDLVFNGFEVGGGSRRIYQREVQEQVFETIGLSPEEAQNKFGFLLEAFEYGTPPHGGIAY 541

Query: 621 GLDRLVMLLADANSIRDVIAFPKTTTAQCALTRAPSEVDPQQLKDLSYKS 670
           GLDRLVML A   SIRDVIAFPKT  A+C LT APS VD +QLK+L   S
Sbjct: 542 GLDRLVMLFAGEESIRDVIAFPKTQQARCLLTDAPSGVDVKQLKELHVAS 589

BLAST of CSPI04G00050 vs. ExPASy Swiss-Prot
Match: Q3MGL3 (Aspartate--tRNA(Asp/Asn) ligase OS=Trichormus variabilis (strain ATCC 29413 / PCC 7937) OX=240292 GN=aspS PE=3 SV=1)

HSP 1 Score: 729.6 bits (1882), Expect = 3.3e-209
Identity = 355/590 (60.17%), Postives = 457/590 (77.46%), Query Frame = 0

Query: 81  RTALCGELSVDDVGKRVRLCGWVALHRVHGGLTFLNLRDHSGIVQVTTLPNEFPDAHSTI 140
           RT  CGEL   D+G+ V L GWV   R HGG+ FL+LRD SGIVQV + P   PD++   
Sbjct: 21  RTHYCGELRQKDIGETVTLYGWVDRRRDHGGVIFLDLRDRSGIVQVVSDPQRTPDSYELA 80

Query: 141 NDLRLEYVVAVEGTVRSRPGDSVNVKMKTGLIEVAAEHVQILNSVGSKLPFLVTTADDAK 200
           N LR EYVV + G V  RP +S+N ++ TG +E+ A+ +++LN V  +LPF V+TAD   
Sbjct: 81  NSLRNEYVVEITGRVTQRPEESLNSRIPTGEVEIYADKIELLNGVRKQLPFQVSTAD--T 140

Query: 201 DSAKEEIRLRYRYLDLRRKQMNFNIMLRHKVVKLLRRYLEDVHGFVEIETPILSRSTPEG 260
           ++ +E++RL+YRYLDLRR +M  NI LRH+VVK +RRYLEDV GF+E+ETPIL+RSTPEG
Sbjct: 141 ETVREDLRLKYRYLDLRRDRMARNIQLRHQVVKAMRRYLEDVEGFIEVETPILTRSTPEG 200

Query: 261 ARDYLVPSRLQPGTFFALPQSPQLFKQMLMVSGFDKYYQIARCFRDEDLRADRQPEFTQL 320
           ARDY++PSR+ PG +FALPQSPQLFKQ+LMVSG D+YYQIARCFRDEDLRADRQPEFTQL
Sbjct: 201 ARDYVLPSRVNPGEWFALPQSPQLFKQILMVSGMDRYYQIARCFRDEDLRADRQPEFTQL 260

Query: 321 DMELSFTTLEDMLRLNEDLIRKVFWEVKGVQLPNPFPRLTYAEAMSRYGSDRPDTRFDLQ 380
           DME+SF + E++++LNE L+  +F  VKGV+LP PFPRLTYAEAM RYG D+PDTR+DLQ
Sbjct: 261 DMEMSFMSEEEIIQLNEKLVSYIFKTVKGVELPLPFPRLTYAEAMERYGCDKPDTRYDLQ 320

Query: 381 LNDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSNTALKK-GDIYTEATKSGAKG 440
           L +VSD+  DS FKVF DA+++GGI+K+L +P+G ++ SN  +K  GD++ EA+++GAKG
Sbjct: 321 LVNVSDVMKDSGFKVFRDAVANGGIVKILPIPNGNEQISNVRIKPGGDLFREASEAGAKG 380

Query: 441 LPFLKVTDDGGIEGIPALVSCLDPTDREEFLNRCSAGPGDLILFAVGHRVSVNKTLDRLR 500
           L +++V +DG I+ I A+   L    ++E L R  A PG L+LF  G   +VNKTLDRLR
Sbjct: 381 LAYIRVREDGEIDTIGAIKDNLSEEQKQEILQRTGAKPGHLLLFGAGDAATVNKTLDRLR 440

Query: 501 LYVANELGLVDHSRHSILWVTDFPMFEWNDLDQRLEALHHPFTAPNPEDVGDLPSARALA 560
             +A E GL+D  + ++LWV DFPMFEWN  ++RLEALHHPFTAP+P+D+ DL +ARA A
Sbjct: 441 QAIAKEFGLIDPDKINLLWVVDFPMFEWNADEKRLEALHHPFTAPHPDDLHDLKTARAQA 500

Query: 561 YDMVYNGVEIGGGSLRIHKREVQEKVLEIVGISLEQAEAKFGYLLEALDMGAPPHGGIAY 620
           YD+V+NG E+GGGS RI++REVQE+V E +G+S E+A+ KFG+LLEA + G PPHGGIAY
Sbjct: 501 YDLVFNGFEVGGGSRRIYQREVQEQVFETIGLSPEEAQNKFGFLLEAFEYGTPPHGGIAY 560

Query: 621 GLDRLVMLLADANSIRDVIAFPKTTTAQCALTRAPSEVDPQQLKDLSYKS 670
           GLDRLVML A   SIRDVIAFPKT  A+C LT APS VD +QLK+L   S
Sbjct: 561 GLDRLVMLFAGEESIRDVIAFPKTQQARCLLTDAPSGVDVKQLKELHVAS 608

BLAST of CSPI04G00050 vs. ExPASy Swiss-Prot
Match: B2J116 (Aspartate--tRNA(Asp/Asn) ligase OS=Nostoc punctiforme (strain ATCC 29133 / PCC 73102) OX=63737 GN=aspS PE=3 SV=1)

HSP 1 Score: 717.6 bits (1851), Expect = 1.3e-205
Identity = 344/590 (58.31%), Postives = 453/590 (76.78%), Query Frame = 0

Query: 81  RTALCGELSVDDVGKRVRLCGWVALHRVHGGLTFLNLRDHSGIVQVTTLPNEFPDAHSTI 140
           RT  CGEL  + +G+ V   GWV   R HGG+ FL+LRD SGIVQ+ + P   PD++   
Sbjct: 2   RTHYCGELRKEHIGETVTFYGWVDRRRDHGGVVFLDLRDRSGIVQIVSDPQRTPDSYEQA 61

Query: 141 NDLRLEYVVAVEGTVRSRPGDSVNVKMKTGLIEVAAEHVQILNSVGSKLPFLVTTADDAK 200
           N LR EYVV + G V  RP +S+N ++ TG +E+ A+ +++LN+VG +LPF V+ AD   
Sbjct: 62  NALRNEYVVEITGRVTQRPEESLNTRIPTGEVEIYADKIKLLNAVGKQLPFQVSVAD--T 121

Query: 201 DSAKEEIRLRYRYLDLRRKQMNFNIMLRHKVVKLLRRYLEDVHGFVEIETPILSRSTPEG 260
           ++ +E++RL+YRYLDLRR++M  N+ LRH++VK +RRYLED+ GF+E+ETPIL+RSTPEG
Sbjct: 122 ETVREDLRLKYRYLDLRRERMAQNLQLRHQIVKAMRRYLEDLEGFIEVETPILTRSTPEG 181

Query: 261 ARDYLVPSRLQPGTFFALPQSPQLFKQMLMVSGFDKYYQIARCFRDEDLRADRQPEFTQL 320
           ARDY++PSR+ PG ++ALPQSPQLFKQ+LMVSG D+YYQIARCFRDEDLRADRQPEFTQL
Sbjct: 182 ARDYVLPSRVNPGEWYALPQSPQLFKQLLMVSGLDRYYQIARCFRDEDLRADRQPEFTQL 241

Query: 321 DMELSFTTLEDMLRLNEDLIRKVFWEVKGVQLPNPFPRLTYAEAMSRYGSDRPDTRFDLQ 380
           DME+SF + E+++ LNE L+  +F  VKG++L  PF RLTYAE M RYGSD+PDTR+ L+
Sbjct: 242 DMEMSFMSQEEIIELNESLVCHIFKTVKGIELQRPFLRLTYAEGMERYGSDKPDTRYGLE 301

Query: 381 LNDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSNTALKK-GDIYTEATKSGAKG 440
           L DVSDI  DS FKVF D +++GGI+K+L +P+G    SN  +K  GD++ EA+++GAKG
Sbjct: 302 LVDVSDIVKDSGFKVFRDTVTNGGIVKILPIPNGNDVISNVRIKPGGDLFKEASEAGAKG 361

Query: 441 LPFLKVTDDGGIEGIPALVSCLDPTDREEFLNRCSAGPGDLILFAVGHRVSVNKTLDRLR 500
           L +++V DDG I+ I A+   L    ++E L R  A  G L+LF  G   +VNKTLDRLR
Sbjct: 362 LAYIRVRDDGEIDTIGAIKDNLSEEQKQEILRRTGAKAGHLLLFGAGEAATVNKTLDRLR 421

Query: 501 LYVANELGLVDHSRHSILWVTDFPMFEWNDLDQRLEALHHPFTAPNPEDVGDLPSARALA 560
             +A E  L+D  + ++LW+TDFPMFEWN  ++RLEALHHPFTAP+P+D+ DL +ARA A
Sbjct: 422 QAIAREFNLIDPDKINLLWITDFPMFEWNADEKRLEALHHPFTAPHPDDLSDLKTARAQA 481

Query: 561 YDMVYNGVEIGGGSLRIHKREVQEKVLEIVGISLEQAEAKFGYLLEALDMGAPPHGGIAY 620
           YD+V NGVE+GGGSLRI++RE+Q++V E +G+S E+A++KFG+LLEA + G PPHGGIAY
Sbjct: 482 YDLVLNGVEVGGGSLRIYQREIQQQVFEAIGLSPEEAQSKFGFLLEAFEYGTPPHGGIAY 541

Query: 621 GLDRLVMLLADANSIRDVIAFPKTTTAQCALTRAPSEVDPQQLKDLSYKS 670
           GLDRLVMLLA   SIRDVIAFPKT  A+C LT APS VD +QLK+L   S
Sbjct: 542 GLDRLVMLLAGEESIRDVIAFPKTQQARCLLTDAPSSVDAKQLKELHVAS 589

BLAST of CSPI04G00050 vs. ExPASy TrEMBL
Match: A0A0A0KT15 (AA_TRNA_LIGASE_II domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G000540 PE=3 SV=1)

HSP 1 Score: 1339.3 bits (3465), Expect = 0.0e+00
Identity = 668/670 (99.70%), Postives = 670/670 (100.00%), Query Frame = 0

Query: 1   MSRSLLRAFPFSTIRPKPPQFLPIRHHISHLRFPQSISTTHRPVSSFSTSATPSPSSATE 60
           MSRSLLRAFPFSTIRPKPPQFLPIRHHISHLRFPQSISTTHRPVSSFSTSATPSPSSATE
Sbjct: 1   MSRSLLRAFPFSTIRPKPPQFLPIRHHISHLRFPQSISTTHRPVSSFSTSATPSPSSATE 60

Query: 61  ILVPTSNPHSPFKDSLQWINRTALCGELSVDDVGKRVRLCGWVALHRVHGGLTFLNLRDH 120
           ILVPTSNPHSPFKDSLQWINRTALCGELSVDDVGKRVRLCGWVALHRVHGGLTFLNLRDH
Sbjct: 61  ILVPTSNPHSPFKDSLQWINRTALCGELSVDDVGKRVRLCGWVALHRVHGGLTFLNLRDH 120

Query: 121 SGIVQVTTLPNEFPDAHSTINDLRLEYVVAVEGTVRSRPGDSVNVKMKTGLIEVAAEHVQ 180
           SGIVQVTTLPNEFPDAHSTINDLRLEYVVAVEGTVRSRPGDSVNVKMKTGLIEVAAEHVQ
Sbjct: 121 SGIVQVTTLPNEFPDAHSTINDLRLEYVVAVEGTVRSRPGDSVNVKMKTGLIEVAAEHVQ 180

Query: 181 ILNSVGSKLPFLVTTADDAKDSAKEEIRLRYRYLDLRRKQMNFNIMLRHKVVKLLRRYLE 240
           ILNSVGSKLPFLVTTADDAKDSAKEEIRLRYRYLDLRRKQMNFNIMLRHKVVKLLRRYLE
Sbjct: 181 ILNSVGSKLPFLVTTADDAKDSAKEEIRLRYRYLDLRRKQMNFNIMLRHKVVKLLRRYLE 240

Query: 241 DVHGFVEIETPILSRSTPEGARDYLVPSRLQPGTFFALPQSPQLFKQMLMVSGFDKYYQI 300
           DVHGFVEIETPILSRSTPEGARDYLVPSR+QPGTFFALPQSPQLFKQMLMVSGFDKYYQI
Sbjct: 241 DVHGFVEIETPILSRSTPEGARDYLVPSRVQPGTFFALPQSPQLFKQMLMVSGFDKYYQI 300

Query: 301 ARCFRDEDLRADRQPEFTQLDMELSFTTLEDMLRLNEDLIRKVFWEVKGVQLPNPFPRLT 360
           ARCFRDEDLRADRQPEFTQLDMELSFTTLEDMLRLNEDLIRKVFWEVKGVQLPNPFPRLT
Sbjct: 301 ARCFRDEDLRADRQPEFTQLDMELSFTTLEDMLRLNEDLIRKVFWEVKGVQLPNPFPRLT 360

Query: 361 YAEAMSRYGSDRPDTRFDLQLNDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSN 420
           YAEAMSRYGSDRPDTRFDLQLNDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSN
Sbjct: 361 YAEAMSRYGSDRPDTRFDLQLNDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSN 420

Query: 421 TALKKGDIYTEATKSGAKGLPFLKVTDDGGIEGIPALVSCLDPTDREEFLNRCSAGPGDL 480
           TALKKGDIYTEATKSGAKGLPFLKVTDDGGIEGIPALVSCLDPTDREEFLNRCSAGPGDL
Sbjct: 421 TALKKGDIYTEATKSGAKGLPFLKVTDDGGIEGIPALVSCLDPTDREEFLNRCSAGPGDL 480

Query: 481 ILFAVGHRVSVNKTLDRLRLYVANELGLVDHSRHSILWVTDFPMFEWNDLDQRLEALHHP 540
           ILFAVGHRVSVNKTLDRLRLYVANELGLVDHSRHSILWVTDFPMFEWNDLDQRLEALHHP
Sbjct: 481 ILFAVGHRVSVNKTLDRLRLYVANELGLVDHSRHSILWVTDFPMFEWNDLDQRLEALHHP 540

Query: 541 FTAPNPEDVGDLPSARALAYDMVYNGVEIGGGSLRIHKREVQEKVLEIVGISLEQAEAKF 600
           FTAPNPEDVGDLPSARALAYDMVYNGVEIGGGSLRIHKREVQEKVLEIVGISLEQAEAKF
Sbjct: 541 FTAPNPEDVGDLPSARALAYDMVYNGVEIGGGSLRIHKREVQEKVLEIVGISLEQAEAKF 600

Query: 601 GYLLEALDMGAPPHGGIAYGLDRLVMLLADANSIRDVIAFPKTTTAQCALTRAPSEVDPQ 660
           GYLLEALDMGAPPHGGIAYGLDRLVMLLADANSIRDVIAFPKTTTAQCALTRAPSEVDPQ
Sbjct: 601 GYLLEALDMGAPPHGGIAYGLDRLVMLLADANSIRDVIAFPKTTTAQCALTRAPSEVDPQ 660

Query: 661 QLKDLSYKSQ 671
           QLKDLSY+SQ
Sbjct: 661 QLKDLSYQSQ 670

BLAST of CSPI04G00050 vs. ExPASy TrEMBL
Match: A0A5D3E1M9 (Aspartate--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G00320 PE=3 SV=1)

HSP 1 Score: 1304.3 bits (3374), Expect = 0.0e+00
Identity = 650/670 (97.01%), Postives = 658/670 (98.21%), Query Frame = 0

Query: 1   MSRSLLRAFPFSTIRPKPPQFLPIRHHISHLRFPQSISTTHRPVSSFSTSATPSPSSATE 60
           MSRSLLRAFPFS IRPKPPQFLPI HHISHLR PQSISTT  PVSSFSTSATP PSSATE
Sbjct: 1   MSRSLLRAFPFSNIRPKPPQFLPILHHISHLRLPQSISTTQTPVSSFSTSATPPPSSATE 60

Query: 61  ILVPTSNPHSPFKDSLQWINRTALCGELSVDDVGKRVRLCGWVALHRVHGGLTFLNLRDH 120
            LVPTSN HSPFKDSLQWINRTALCGELSVDDVGKRVRLCGWVALHRVHGGLTFLNLRDH
Sbjct: 61  TLVPTSNSHSPFKDSLQWINRTALCGELSVDDVGKRVRLCGWVALHRVHGGLTFLNLRDH 120

Query: 121 SGIVQVTTLPNEFPDAHSTINDLRLEYVVAVEGTVRSRPGDSVNVKMKTGLIEVAAEHVQ 180
           SGIVQVTTLPNEFPDAHS INDLRLEYVVAVEGTVRSRPGDSVNVKMKTG IEVAAEHVQ
Sbjct: 121 SGIVQVTTLPNEFPDAHSAINDLRLEYVVAVEGTVRSRPGDSVNVKMKTGFIEVAAEHVQ 180

Query: 181 ILNSVGSKLPFLVTTADDAKDSAKEEIRLRYRYLDLRRKQMNFNIMLRHKVVKLLRRYLE 240
           ILNSVGSKLPFLVTTADDAKDSAKEEIRLRYRYLDLRRKQMNFNIMLRHKVVKL+RRYLE
Sbjct: 181 ILNSVGSKLPFLVTTADDAKDSAKEEIRLRYRYLDLRRKQMNFNIMLRHKVVKLIRRYLE 240

Query: 241 DVHGFVEIETPILSRSTPEGARDYLVPSRLQPGTFFALPQSPQLFKQMLMVSGFDKYYQI 300
           DVHGFVEIETPILSRSTPEGARDYLVPSR+QPGTFFALPQSPQLFKQMLMVSGFDKYYQI
Sbjct: 241 DVHGFVEIETPILSRSTPEGARDYLVPSRVQPGTFFALPQSPQLFKQMLMVSGFDKYYQI 300

Query: 301 ARCFRDEDLRADRQPEFTQLDMELSFTTLEDMLRLNEDLIRKVFWEVKGVQLPNPFPRLT 360
           ARCFRDEDLRADRQPEFTQLDMELSFTTLEDMLRLNEDLIRKVFWEVKGVQLPNPFPRLT
Sbjct: 301 ARCFRDEDLRADRQPEFTQLDMELSFTTLEDMLRLNEDLIRKVFWEVKGVQLPNPFPRLT 360

Query: 361 YAEAMSRYGSDRPDTRFDLQLNDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSN 420
           YAEAMSRYGSDRPDTRFDLQLNDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSN
Sbjct: 361 YAEAMSRYGSDRPDTRFDLQLNDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSN 420

Query: 421 TALKKGDIYTEATKSGAKGLPFLKVTDDGGIEGIPALVSCLDPTDREEFLNRCSAGPGDL 480
           TALKKGDIYTEA KSGAKGLPFLKVTDDGGIEGIPALVSCLDPT RE+FLNRCSAGPGDL
Sbjct: 421 TALKKGDIYTEAMKSGAKGLPFLKVTDDGGIEGIPALVSCLDPTVREKFLNRCSAGPGDL 480

Query: 481 ILFAVGHRVSVNKTLDRLRLYVANELGLVDHSRHSILWVTDFPMFEWNDLDQRLEALHHP 540
           +LFAVGHRVSVNKTLDRLRLYVANELGLVDHSRHSILWVTDFPMFEWNDL+QRLEALHHP
Sbjct: 481 VLFAVGHRVSVNKTLDRLRLYVANELGLVDHSRHSILWVTDFPMFEWNDLEQRLEALHHP 540

Query: 541 FTAPNPEDVGDLPSARALAYDMVYNGVEIGGGSLRIHKREVQEKVLEIVGISLEQAEAKF 600
           FTAPNPEDVGDLPSARALAYDMVYNGVEIGGGSLRIHKREVQEKVLEIVGISLEQAEAKF
Sbjct: 541 FTAPNPEDVGDLPSARALAYDMVYNGVEIGGGSLRIHKREVQEKVLEIVGISLEQAEAKF 600

Query: 601 GYLLEALDMGAPPHGGIAYGLDRLVMLLADANSIRDVIAFPKTTTAQCALTRAPSEVDPQ 660
           GYLLEALDMGAPPHGGIAYG+DRLVMLLADANSIRDVIAFPKTTTAQCALTRAPSEVDPQ
Sbjct: 601 GYLLEALDMGAPPHGGIAYGVDRLVMLLADANSIRDVIAFPKTTTAQCALTRAPSEVDPQ 660

Query: 661 QLKDLSYKSQ 671
           QLKDLSY++Q
Sbjct: 661 QLKDLSYQTQ 670

BLAST of CSPI04G00050 vs. ExPASy TrEMBL
Match: A0A1S3BZT5 (aspartate--tRNA ligase, chloroplastic/mitochondrial isoform X1 OS=Cucumis melo OX=3656 GN=LOC103494835 PE=3 SV=1)

HSP 1 Score: 1304.3 bits (3374), Expect = 0.0e+00
Identity = 650/670 (97.01%), Postives = 658/670 (98.21%), Query Frame = 0

Query: 1   MSRSLLRAFPFSTIRPKPPQFLPIRHHISHLRFPQSISTTHRPVSSFSTSATPSPSSATE 60
           MSRSLLRAFPFS IRPKPPQFLPI HHISHLR PQSISTT  PVSSFSTSATP PSSATE
Sbjct: 1   MSRSLLRAFPFSNIRPKPPQFLPILHHISHLRLPQSISTTQTPVSSFSTSATPPPSSATE 60

Query: 61  ILVPTSNPHSPFKDSLQWINRTALCGELSVDDVGKRVRLCGWVALHRVHGGLTFLNLRDH 120
            LVPTSN HSPFKDSLQWINRTALCGELSVDDVGKRVRLCGWVALHRVHGGLTFLNLRDH
Sbjct: 61  TLVPTSNSHSPFKDSLQWINRTALCGELSVDDVGKRVRLCGWVALHRVHGGLTFLNLRDH 120

Query: 121 SGIVQVTTLPNEFPDAHSTINDLRLEYVVAVEGTVRSRPGDSVNVKMKTGLIEVAAEHVQ 180
           SGIVQVTTLPNEFPDAHS INDLRLEYVVAVEGTVRSRPGDSVNVKMKTG IEVAAEHVQ
Sbjct: 121 SGIVQVTTLPNEFPDAHSAINDLRLEYVVAVEGTVRSRPGDSVNVKMKTGFIEVAAEHVQ 180

Query: 181 ILNSVGSKLPFLVTTADDAKDSAKEEIRLRYRYLDLRRKQMNFNIMLRHKVVKLLRRYLE 240
           ILNSVGSKLPFLVTTADDAKDSAKEEIRLRYRYLDLRRKQMNFNIMLRHKVVKL+RRYLE
Sbjct: 181 ILNSVGSKLPFLVTTADDAKDSAKEEIRLRYRYLDLRRKQMNFNIMLRHKVVKLIRRYLE 240

Query: 241 DVHGFVEIETPILSRSTPEGARDYLVPSRLQPGTFFALPQSPQLFKQMLMVSGFDKYYQI 300
           DVHGFVEIETPILSRSTPEGARDYLVPSR+QPGTFFALPQSPQLFKQMLMVSGFDKYYQI
Sbjct: 241 DVHGFVEIETPILSRSTPEGARDYLVPSRVQPGTFFALPQSPQLFKQMLMVSGFDKYYQI 300

Query: 301 ARCFRDEDLRADRQPEFTQLDMELSFTTLEDMLRLNEDLIRKVFWEVKGVQLPNPFPRLT 360
           ARCFRDEDLRADRQPEFTQLDMELSFTTLEDMLRLNEDLIRKVFWEVKGVQLPNPFPRLT
Sbjct: 301 ARCFRDEDLRADRQPEFTQLDMELSFTTLEDMLRLNEDLIRKVFWEVKGVQLPNPFPRLT 360

Query: 361 YAEAMSRYGSDRPDTRFDLQLNDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSN 420
           YAEAMSRYGSDRPDTRFDLQLNDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSN
Sbjct: 361 YAEAMSRYGSDRPDTRFDLQLNDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSN 420

Query: 421 TALKKGDIYTEATKSGAKGLPFLKVTDDGGIEGIPALVSCLDPTDREEFLNRCSAGPGDL 480
           TALKKGDIYTEA KSGAKGLPFLKVTDDGGIEGIPALVSCLDPT RE+FLNRCSAGPGDL
Sbjct: 421 TALKKGDIYTEAMKSGAKGLPFLKVTDDGGIEGIPALVSCLDPTVREKFLNRCSAGPGDL 480

Query: 481 ILFAVGHRVSVNKTLDRLRLYVANELGLVDHSRHSILWVTDFPMFEWNDLDQRLEALHHP 540
           +LFAVGHRVSVNKTLDRLRLYVANELGLVDHSRHSILWVTDFPMFEWNDL+QRLEALHHP
Sbjct: 481 VLFAVGHRVSVNKTLDRLRLYVANELGLVDHSRHSILWVTDFPMFEWNDLEQRLEALHHP 540

Query: 541 FTAPNPEDVGDLPSARALAYDMVYNGVEIGGGSLRIHKREVQEKVLEIVGISLEQAEAKF 600
           FTAPNPEDVGDLPSARALAYDMVYNGVEIGGGSLRIHKREVQEKVLEIVGISLEQAEAKF
Sbjct: 541 FTAPNPEDVGDLPSARALAYDMVYNGVEIGGGSLRIHKREVQEKVLEIVGISLEQAEAKF 600

Query: 601 GYLLEALDMGAPPHGGIAYGLDRLVMLLADANSIRDVIAFPKTTTAQCALTRAPSEVDPQ 660
           GYLLEALDMGAPPHGGIAYG+DRLVMLLADANSIRDVIAFPKTTTAQCALTRAPSEVDPQ
Sbjct: 601 GYLLEALDMGAPPHGGIAYGVDRLVMLLADANSIRDVIAFPKTTTAQCALTRAPSEVDPQ 660

Query: 661 QLKDLSYKSQ 671
           QLKDLSY++Q
Sbjct: 661 QLKDLSYQTQ 670

BLAST of CSPI04G00050 vs. ExPASy TrEMBL
Match: A0A5A7TRV5 (Aspartate--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G00320 PE=3 SV=1)

HSP 1 Score: 1292.7 bits (3344), Expect = 0.0e+00
Identity = 647/670 (96.57%), Postives = 655/670 (97.76%), Query Frame = 0

Query: 1   MSRSLLRAFPFSTIRPKPPQFLPIRHHISHLRFPQSISTTHRPVSSFSTSATPSPSSATE 60
           MSRSLLRAFPFS IRPKPPQFLPI HHISHLR PQSISTT   VSSFSTSATP PSSATE
Sbjct: 1   MSRSLLRAFPFSNIRPKPPQFLPILHHISHLRLPQSISTTQTSVSSFSTSATPPPSSATE 60

Query: 61  ILVPTSNPHSPFKDSLQWINRTALCGELSVDDVGKRVRLCGWVALHRVHGGLTFLNLRDH 120
            LVPTSN HSPFKDSLQWINRTALCGELSVDDVGKRVRLCGWVALHRVHGGLTFLNLRDH
Sbjct: 61  TLVPTSNSHSPFKDSLQWINRTALCGELSVDDVGKRVRLCGWVALHRVHGGLTFLNLRDH 120

Query: 121 SGIVQVTTLPNEFPDAHSTINDLRLEYVVAVEGTVRSRPGDSVNVKMKTGLIEVAAEHVQ 180
           SGI  VTTLPNEFPDAHS INDLRLEYVVAVEGTVRSRPGDSVNVKMKTG IEVAAEHVQ
Sbjct: 121 SGI--VTTLPNEFPDAHSAINDLRLEYVVAVEGTVRSRPGDSVNVKMKTGFIEVAAEHVQ 180

Query: 181 ILNSVGSKLPFLVTTADDAKDSAKEEIRLRYRYLDLRRKQMNFNIMLRHKVVKLLRRYLE 240
           ILNSVGSKLPFLVTTADDAKDSAKEEIRLRYRYLDLRRKQMNFNIMLRHKVVKL+RRYLE
Sbjct: 181 ILNSVGSKLPFLVTTADDAKDSAKEEIRLRYRYLDLRRKQMNFNIMLRHKVVKLIRRYLE 240

Query: 241 DVHGFVEIETPILSRSTPEGARDYLVPSRLQPGTFFALPQSPQLFKQMLMVSGFDKYYQI 300
           DVHGFVEIETPILSRSTPEGARDYLVPSR+QPGTFFALPQSPQLFKQMLMVSGFDKYYQI
Sbjct: 241 DVHGFVEIETPILSRSTPEGARDYLVPSRVQPGTFFALPQSPQLFKQMLMVSGFDKYYQI 300

Query: 301 ARCFRDEDLRADRQPEFTQLDMELSFTTLEDMLRLNEDLIRKVFWEVKGVQLPNPFPRLT 360
           ARCFRDEDLRADRQPEFTQLDMELSFTTLEDMLRLNEDLIRKVFWEVKGVQLPNPFPRLT
Sbjct: 301 ARCFRDEDLRADRQPEFTQLDMELSFTTLEDMLRLNEDLIRKVFWEVKGVQLPNPFPRLT 360

Query: 361 YAEAMSRYGSDRPDTRFDLQLNDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSN 420
           YAEAMSRYGSDRPDTRFDLQLNDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSN
Sbjct: 361 YAEAMSRYGSDRPDTRFDLQLNDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSN 420

Query: 421 TALKKGDIYTEATKSGAKGLPFLKVTDDGGIEGIPALVSCLDPTDREEFLNRCSAGPGDL 480
           TALKKGDIYTEA KSGAKGLPFLKVTDDGGIEGIPALVSCLDPT RE+FLNRCSAGPGDL
Sbjct: 421 TALKKGDIYTEAMKSGAKGLPFLKVTDDGGIEGIPALVSCLDPTVREKFLNRCSAGPGDL 480

Query: 481 ILFAVGHRVSVNKTLDRLRLYVANELGLVDHSRHSILWVTDFPMFEWNDLDQRLEALHHP 540
           +LFAVGHRVSVNKTLDRLRLYVANELGLVDHSRHSILWVTDFPMFEWNDL+QRLEALHHP
Sbjct: 481 VLFAVGHRVSVNKTLDRLRLYVANELGLVDHSRHSILWVTDFPMFEWNDLEQRLEALHHP 540

Query: 541 FTAPNPEDVGDLPSARALAYDMVYNGVEIGGGSLRIHKREVQEKVLEIVGISLEQAEAKF 600
           FTAPNPEDVGDLPSARALAYDMVYNGVEIGGGSLRIHKREVQEKVLEIVGISLEQAEAKF
Sbjct: 541 FTAPNPEDVGDLPSARALAYDMVYNGVEIGGGSLRIHKREVQEKVLEIVGISLEQAEAKF 600

Query: 601 GYLLEALDMGAPPHGGIAYGLDRLVMLLADANSIRDVIAFPKTTTAQCALTRAPSEVDPQ 660
           GYLLEALDMGAPPHGGIAYG+DRLVMLLADANSIRDVIAFPKTTTAQCALTRAPSEVDPQ
Sbjct: 601 GYLLEALDMGAPPHGGIAYGVDRLVMLLADANSIRDVIAFPKTTTAQCALTRAPSEVDPQ 660

Query: 661 QLKDLSYKSQ 671
           QLKDLSY++Q
Sbjct: 661 QLKDLSYQTQ 668

BLAST of CSPI04G00050 vs. ExPASy TrEMBL
Match: A0A6J1D6F8 (aspartate--tRNA ligase, chloroplastic/mitochondrial OS=Momordica charantia OX=3673 GN=LOC111017747 PE=3 SV=1)

HSP 1 Score: 1225.7 bits (3170), Expect = 0.0e+00
Identity = 614/671 (91.51%), Postives = 634/671 (94.49%), Query Frame = 0

Query: 1   MSRSLLRAFPF-STIRPKPPQFLPIRHHISHLRFPQSISTTHRPVSSFSTSATPSPSSAT 60
           MSRSLLRAFPF ST+R KPPQFLP    ISHLR  +SIST HR VSSFS SAT  PSS  
Sbjct: 1   MSRSLLRAFPFSSTLRQKPPQFLPTFRRISHLRRAKSISTNHRAVSSFSASATHQPSSVA 60

Query: 61  EILVPTSNPHSPFKDSLQWINRTALCGELSVDDVGKRVRLCGWVALHRVHGGLTFLNLRD 120
           E L P SN  SP K+SL+WINRTALCGELSVDDVGKRVRLCGWVALHRVHGGLTFLNLRD
Sbjct: 61  ETLAPCSNSQSPLKESLRWINRTALCGELSVDDVGKRVRLCGWVALHRVHGGLTFLNLRD 120

Query: 121 HSGIVQVTTLPNEFPDAHSTINDLRLEYVVAVEGTVRSRPGDSVNVKMKTGLIEVAAEHV 180
           HSGIVQVTTLPNEFPDAHS +NDLRLEYVVAVEG VRSRPG+S+N KMKTGLIEVAAEHV
Sbjct: 121 HSGIVQVTTLPNEFPDAHSAVNDLRLEYVVAVEGVVRSRPGNSINAKMKTGLIEVAAEHV 180

Query: 181 QILNSVGSKLPFLVTTADDAKDSAKEEIRLRYRYLDLRRKQMNFNIMLRHKVVKLLRRYL 240
           QILNSVGSKLPFLVTTA+D KDSAKEEIRLRYRYLDLRRKQMNFNIMLRHKVVKLLRRYL
Sbjct: 181 QILNSVGSKLPFLVTTAEDTKDSAKEEIRLRYRYLDLRRKQMNFNIMLRHKVVKLLRRYL 240

Query: 241 EDVHGFVEIETPILSRSTPEGARDYLVPSRLQPGTFFALPQSPQLFKQMLMVSGFDKYYQ 300
           EDVHGFVEIETPILSRSTPEGARDYLVPSR+QPGTF+ALPQSPQLFKQMLMVSGFDKYYQ
Sbjct: 241 EDVHGFVEIETPILSRSTPEGARDYLVPSRVQPGTFYALPQSPQLFKQMLMVSGFDKYYQ 300

Query: 301 IARCFRDEDLRADRQPEFTQLDMELSFTTLEDMLRLNEDLIRKVFWEVKGVQLPNPFPRL 360
           IARCFRDEDLRADRQPEFTQLDMEL+FTTLEDMLRLNE+LIRKVFWEVKGVQLPNPFPRL
Sbjct: 301 IARCFRDEDLRADRQPEFTQLDMELAFTTLEDMLRLNEELIRKVFWEVKGVQLPNPFPRL 360

Query: 361 TYAEAMSRYGSDRPDTRFDLQLNDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYS 420
           TYAEAMSRYGSDRPDTRFDLQL DVSDI ADSPF+VFADALSSGGIIKVLCVPSGAKRYS
Sbjct: 361 TYAEAMSRYGSDRPDTRFDLQLQDVSDILADSPFRVFADALSSGGIIKVLCVPSGAKRYS 420

Query: 421 NTALKKGDIYTEATKSGAKGLPFLKVTDDGGIEGIPALVSCLDPTDREEFLNRCSAGPGD 480
           NTALKKGDIY+EA KSGAKGLPFLKV D GGIEGIPALVS LDP  REEFL RCSAGPGD
Sbjct: 421 NTALKKGDIYSEAIKSGAKGLPFLKVLDGGGIEGIPALVSSLDPAGREEFLRRCSAGPGD 480

Query: 481 LILFAVGHRVSVNKTLDRLRLYVANELGLVDHSRHSILWVTDFPMFEWNDLDQRLEALHH 540
           L+LFAVGHR SVNKTLDRLRLYVANELGLVDHSRHSILWVTDFPMFEWNDL+QRLEALHH
Sbjct: 481 LVLFAVGHRASVNKTLDRLRLYVANELGLVDHSRHSILWVTDFPMFEWNDLEQRLEALHH 540

Query: 541 PFTAPNPEDVGDLPSARALAYDMVYNGVEIGGGSLRIHKREVQEKVLEIVGISLEQAEAK 600
           PFTAPNPEDV DLPSARALAYDMVYNGVEIGGGSLRIHKREVQEKVL+IVGIS EQAEAK
Sbjct: 541 PFTAPNPEDVDDLPSARALAYDMVYNGVEIGGGSLRIHKREVQEKVLKIVGISPEQAEAK 600

Query: 601 FGYLLEALDMGAPPHGGIAYGLDRLVMLLADANSIRDVIAFPKTTTAQCALTRAPSEVDP 660
           FGYLLEALDMGAPPHGGIAYGLDRLVMLLADANSIRDVIAFPKTTTAQCALT+APSEVDP
Sbjct: 601 FGYLLEALDMGAPPHGGIAYGLDRLVMLLADANSIRDVIAFPKTTTAQCALTKAPSEVDP 660

Query: 661 QQLKDLSYKSQ 671
           QQLKDLSY++Q
Sbjct: 661 QQLKDLSYQTQ 671

BLAST of CSPI04G00050 vs. NCBI nr
Match: XP_004150278.2 (aspartate--tRNA ligase, chloroplastic/mitochondrial [Cucumis sativus] >KGN52733.1 hypothetical protein Csa_014989 [Cucumis sativus])

HSP 1 Score: 1339.3 bits (3465), Expect = 0.0e+00
Identity = 668/670 (99.70%), Postives = 670/670 (100.00%), Query Frame = 0

Query: 1   MSRSLLRAFPFSTIRPKPPQFLPIRHHISHLRFPQSISTTHRPVSSFSTSATPSPSSATE 60
           MSRSLLRAFPFSTIRPKPPQFLPIRHHISHLRFPQSISTTHRPVSSFSTSATPSPSSATE
Sbjct: 1   MSRSLLRAFPFSTIRPKPPQFLPIRHHISHLRFPQSISTTHRPVSSFSTSATPSPSSATE 60

Query: 61  ILVPTSNPHSPFKDSLQWINRTALCGELSVDDVGKRVRLCGWVALHRVHGGLTFLNLRDH 120
           ILVPTSNPHSPFKDSLQWINRTALCGELSVDDVGKRVRLCGWVALHRVHGGLTFLNLRDH
Sbjct: 61  ILVPTSNPHSPFKDSLQWINRTALCGELSVDDVGKRVRLCGWVALHRVHGGLTFLNLRDH 120

Query: 121 SGIVQVTTLPNEFPDAHSTINDLRLEYVVAVEGTVRSRPGDSVNVKMKTGLIEVAAEHVQ 180
           SGIVQVTTLPNEFPDAHSTINDLRLEYVVAVEGTVRSRPGDSVNVKMKTGLIEVAAEHVQ
Sbjct: 121 SGIVQVTTLPNEFPDAHSTINDLRLEYVVAVEGTVRSRPGDSVNVKMKTGLIEVAAEHVQ 180

Query: 181 ILNSVGSKLPFLVTTADDAKDSAKEEIRLRYRYLDLRRKQMNFNIMLRHKVVKLLRRYLE 240
           ILNSVGSKLPFLVTTADDAKDSAKEEIRLRYRYLDLRRKQMNFNIMLRHKVVKLLRRYLE
Sbjct: 181 ILNSVGSKLPFLVTTADDAKDSAKEEIRLRYRYLDLRRKQMNFNIMLRHKVVKLLRRYLE 240

Query: 241 DVHGFVEIETPILSRSTPEGARDYLVPSRLQPGTFFALPQSPQLFKQMLMVSGFDKYYQI 300
           DVHGFVEIETPILSRSTPEGARDYLVPSR+QPGTFFALPQSPQLFKQMLMVSGFDKYYQI
Sbjct: 241 DVHGFVEIETPILSRSTPEGARDYLVPSRVQPGTFFALPQSPQLFKQMLMVSGFDKYYQI 300

Query: 301 ARCFRDEDLRADRQPEFTQLDMELSFTTLEDMLRLNEDLIRKVFWEVKGVQLPNPFPRLT 360
           ARCFRDEDLRADRQPEFTQLDMELSFTTLEDMLRLNEDLIRKVFWEVKGVQLPNPFPRLT
Sbjct: 301 ARCFRDEDLRADRQPEFTQLDMELSFTTLEDMLRLNEDLIRKVFWEVKGVQLPNPFPRLT 360

Query: 361 YAEAMSRYGSDRPDTRFDLQLNDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSN 420
           YAEAMSRYGSDRPDTRFDLQLNDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSN
Sbjct: 361 YAEAMSRYGSDRPDTRFDLQLNDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSN 420

Query: 421 TALKKGDIYTEATKSGAKGLPFLKVTDDGGIEGIPALVSCLDPTDREEFLNRCSAGPGDL 480
           TALKKGDIYTEATKSGAKGLPFLKVTDDGGIEGIPALVSCLDPTDREEFLNRCSAGPGDL
Sbjct: 421 TALKKGDIYTEATKSGAKGLPFLKVTDDGGIEGIPALVSCLDPTDREEFLNRCSAGPGDL 480

Query: 481 ILFAVGHRVSVNKTLDRLRLYVANELGLVDHSRHSILWVTDFPMFEWNDLDQRLEALHHP 540
           ILFAVGHRVSVNKTLDRLRLYVANELGLVDHSRHSILWVTDFPMFEWNDLDQRLEALHHP
Sbjct: 481 ILFAVGHRVSVNKTLDRLRLYVANELGLVDHSRHSILWVTDFPMFEWNDLDQRLEALHHP 540

Query: 541 FTAPNPEDVGDLPSARALAYDMVYNGVEIGGGSLRIHKREVQEKVLEIVGISLEQAEAKF 600
           FTAPNPEDVGDLPSARALAYDMVYNGVEIGGGSLRIHKREVQEKVLEIVGISLEQAEAKF
Sbjct: 541 FTAPNPEDVGDLPSARALAYDMVYNGVEIGGGSLRIHKREVQEKVLEIVGISLEQAEAKF 600

Query: 601 GYLLEALDMGAPPHGGIAYGLDRLVMLLADANSIRDVIAFPKTTTAQCALTRAPSEVDPQ 660
           GYLLEALDMGAPPHGGIAYGLDRLVMLLADANSIRDVIAFPKTTTAQCALTRAPSEVDPQ
Sbjct: 601 GYLLEALDMGAPPHGGIAYGLDRLVMLLADANSIRDVIAFPKTTTAQCALTRAPSEVDPQ 660

Query: 661 QLKDLSYKSQ 671
           QLKDLSY+SQ
Sbjct: 661 QLKDLSYQSQ 670

BLAST of CSPI04G00050 vs. NCBI nr
Match: XP_008454426.1 (PREDICTED: aspartate--tRNA ligase, chloroplastic/mitochondrial isoform X1 [Cucumis melo] >TYK29440.1 aspartate--tRNA ligase [Cucumis melo var. makuwa])

HSP 1 Score: 1304.3 bits (3374), Expect = 0.0e+00
Identity = 650/670 (97.01%), Postives = 658/670 (98.21%), Query Frame = 0

Query: 1   MSRSLLRAFPFSTIRPKPPQFLPIRHHISHLRFPQSISTTHRPVSSFSTSATPSPSSATE 60
           MSRSLLRAFPFS IRPKPPQFLPI HHISHLR PQSISTT  PVSSFSTSATP PSSATE
Sbjct: 1   MSRSLLRAFPFSNIRPKPPQFLPILHHISHLRLPQSISTTQTPVSSFSTSATPPPSSATE 60

Query: 61  ILVPTSNPHSPFKDSLQWINRTALCGELSVDDVGKRVRLCGWVALHRVHGGLTFLNLRDH 120
            LVPTSN HSPFKDSLQWINRTALCGELSVDDVGKRVRLCGWVALHRVHGGLTFLNLRDH
Sbjct: 61  TLVPTSNSHSPFKDSLQWINRTALCGELSVDDVGKRVRLCGWVALHRVHGGLTFLNLRDH 120

Query: 121 SGIVQVTTLPNEFPDAHSTINDLRLEYVVAVEGTVRSRPGDSVNVKMKTGLIEVAAEHVQ 180
           SGIVQVTTLPNEFPDAHS INDLRLEYVVAVEGTVRSRPGDSVNVKMKTG IEVAAEHVQ
Sbjct: 121 SGIVQVTTLPNEFPDAHSAINDLRLEYVVAVEGTVRSRPGDSVNVKMKTGFIEVAAEHVQ 180

Query: 181 ILNSVGSKLPFLVTTADDAKDSAKEEIRLRYRYLDLRRKQMNFNIMLRHKVVKLLRRYLE 240
           ILNSVGSKLPFLVTTADDAKDSAKEEIRLRYRYLDLRRKQMNFNIMLRHKVVKL+RRYLE
Sbjct: 181 ILNSVGSKLPFLVTTADDAKDSAKEEIRLRYRYLDLRRKQMNFNIMLRHKVVKLIRRYLE 240

Query: 241 DVHGFVEIETPILSRSTPEGARDYLVPSRLQPGTFFALPQSPQLFKQMLMVSGFDKYYQI 300
           DVHGFVEIETPILSRSTPEGARDYLVPSR+QPGTFFALPQSPQLFKQMLMVSGFDKYYQI
Sbjct: 241 DVHGFVEIETPILSRSTPEGARDYLVPSRVQPGTFFALPQSPQLFKQMLMVSGFDKYYQI 300

Query: 301 ARCFRDEDLRADRQPEFTQLDMELSFTTLEDMLRLNEDLIRKVFWEVKGVQLPNPFPRLT 360
           ARCFRDEDLRADRQPEFTQLDMELSFTTLEDMLRLNEDLIRKVFWEVKGVQLPNPFPRLT
Sbjct: 301 ARCFRDEDLRADRQPEFTQLDMELSFTTLEDMLRLNEDLIRKVFWEVKGVQLPNPFPRLT 360

Query: 361 YAEAMSRYGSDRPDTRFDLQLNDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSN 420
           YAEAMSRYGSDRPDTRFDLQLNDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSN
Sbjct: 361 YAEAMSRYGSDRPDTRFDLQLNDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSN 420

Query: 421 TALKKGDIYTEATKSGAKGLPFLKVTDDGGIEGIPALVSCLDPTDREEFLNRCSAGPGDL 480
           TALKKGDIYTEA KSGAKGLPFLKVTDDGGIEGIPALVSCLDPT RE+FLNRCSAGPGDL
Sbjct: 421 TALKKGDIYTEAMKSGAKGLPFLKVTDDGGIEGIPALVSCLDPTVREKFLNRCSAGPGDL 480

Query: 481 ILFAVGHRVSVNKTLDRLRLYVANELGLVDHSRHSILWVTDFPMFEWNDLDQRLEALHHP 540
           +LFAVGHRVSVNKTLDRLRLYVANELGLVDHSRHSILWVTDFPMFEWNDL+QRLEALHHP
Sbjct: 481 VLFAVGHRVSVNKTLDRLRLYVANELGLVDHSRHSILWVTDFPMFEWNDLEQRLEALHHP 540

Query: 541 FTAPNPEDVGDLPSARALAYDMVYNGVEIGGGSLRIHKREVQEKVLEIVGISLEQAEAKF 600
           FTAPNPEDVGDLPSARALAYDMVYNGVEIGGGSLRIHKREVQEKVLEIVGISLEQAEAKF
Sbjct: 541 FTAPNPEDVGDLPSARALAYDMVYNGVEIGGGSLRIHKREVQEKVLEIVGISLEQAEAKF 600

Query: 601 GYLLEALDMGAPPHGGIAYGLDRLVMLLADANSIRDVIAFPKTTTAQCALTRAPSEVDPQ 660
           GYLLEALDMGAPPHGGIAYG+DRLVMLLADANSIRDVIAFPKTTTAQCALTRAPSEVDPQ
Sbjct: 601 GYLLEALDMGAPPHGGIAYGVDRLVMLLADANSIRDVIAFPKTTTAQCALTRAPSEVDPQ 660

Query: 661 QLKDLSYKSQ 671
           QLKDLSY++Q
Sbjct: 661 QLKDLSYQTQ 670

BLAST of CSPI04G00050 vs. NCBI nr
Match: KAA0044311.1 (aspartate--tRNA ligase [Cucumis melo var. makuwa])

HSP 1 Score: 1292.7 bits (3344), Expect = 0.0e+00
Identity = 647/670 (96.57%), Postives = 655/670 (97.76%), Query Frame = 0

Query: 1   MSRSLLRAFPFSTIRPKPPQFLPIRHHISHLRFPQSISTTHRPVSSFSTSATPSPSSATE 60
           MSRSLLRAFPFS IRPKPPQFLPI HHISHLR PQSISTT   VSSFSTSATP PSSATE
Sbjct: 1   MSRSLLRAFPFSNIRPKPPQFLPILHHISHLRLPQSISTTQTSVSSFSTSATPPPSSATE 60

Query: 61  ILVPTSNPHSPFKDSLQWINRTALCGELSVDDVGKRVRLCGWVALHRVHGGLTFLNLRDH 120
            LVPTSN HSPFKDSLQWINRTALCGELSVDDVGKRVRLCGWVALHRVHGGLTFLNLRDH
Sbjct: 61  TLVPTSNSHSPFKDSLQWINRTALCGELSVDDVGKRVRLCGWVALHRVHGGLTFLNLRDH 120

Query: 121 SGIVQVTTLPNEFPDAHSTINDLRLEYVVAVEGTVRSRPGDSVNVKMKTGLIEVAAEHVQ 180
           SGI  VTTLPNEFPDAHS INDLRLEYVVAVEGTVRSRPGDSVNVKMKTG IEVAAEHVQ
Sbjct: 121 SGI--VTTLPNEFPDAHSAINDLRLEYVVAVEGTVRSRPGDSVNVKMKTGFIEVAAEHVQ 180

Query: 181 ILNSVGSKLPFLVTTADDAKDSAKEEIRLRYRYLDLRRKQMNFNIMLRHKVVKLLRRYLE 240
           ILNSVGSKLPFLVTTADDAKDSAKEEIRLRYRYLDLRRKQMNFNIMLRHKVVKL+RRYLE
Sbjct: 181 ILNSVGSKLPFLVTTADDAKDSAKEEIRLRYRYLDLRRKQMNFNIMLRHKVVKLIRRYLE 240

Query: 241 DVHGFVEIETPILSRSTPEGARDYLVPSRLQPGTFFALPQSPQLFKQMLMVSGFDKYYQI 300
           DVHGFVEIETPILSRSTPEGARDYLVPSR+QPGTFFALPQSPQLFKQMLMVSGFDKYYQI
Sbjct: 241 DVHGFVEIETPILSRSTPEGARDYLVPSRVQPGTFFALPQSPQLFKQMLMVSGFDKYYQI 300

Query: 301 ARCFRDEDLRADRQPEFTQLDMELSFTTLEDMLRLNEDLIRKVFWEVKGVQLPNPFPRLT 360
           ARCFRDEDLRADRQPEFTQLDMELSFTTLEDMLRLNEDLIRKVFWEVKGVQLPNPFPRLT
Sbjct: 301 ARCFRDEDLRADRQPEFTQLDMELSFTTLEDMLRLNEDLIRKVFWEVKGVQLPNPFPRLT 360

Query: 361 YAEAMSRYGSDRPDTRFDLQLNDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSN 420
           YAEAMSRYGSDRPDTRFDLQLNDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSN
Sbjct: 361 YAEAMSRYGSDRPDTRFDLQLNDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSN 420

Query: 421 TALKKGDIYTEATKSGAKGLPFLKVTDDGGIEGIPALVSCLDPTDREEFLNRCSAGPGDL 480
           TALKKGDIYTEA KSGAKGLPFLKVTDDGGIEGIPALVSCLDPT RE+FLNRCSAGPGDL
Sbjct: 421 TALKKGDIYTEAMKSGAKGLPFLKVTDDGGIEGIPALVSCLDPTVREKFLNRCSAGPGDL 480

Query: 481 ILFAVGHRVSVNKTLDRLRLYVANELGLVDHSRHSILWVTDFPMFEWNDLDQRLEALHHP 540
           +LFAVGHRVSVNKTLDRLRLYVANELGLVDHSRHSILWVTDFPMFEWNDL+QRLEALHHP
Sbjct: 481 VLFAVGHRVSVNKTLDRLRLYVANELGLVDHSRHSILWVTDFPMFEWNDLEQRLEALHHP 540

Query: 541 FTAPNPEDVGDLPSARALAYDMVYNGVEIGGGSLRIHKREVQEKVLEIVGISLEQAEAKF 600
           FTAPNPEDVGDLPSARALAYDMVYNGVEIGGGSLRIHKREVQEKVLEIVGISLEQAEAKF
Sbjct: 541 FTAPNPEDVGDLPSARALAYDMVYNGVEIGGGSLRIHKREVQEKVLEIVGISLEQAEAKF 600

Query: 601 GYLLEALDMGAPPHGGIAYGLDRLVMLLADANSIRDVIAFPKTTTAQCALTRAPSEVDPQ 660
           GYLLEALDMGAPPHGGIAYG+DRLVMLLADANSIRDVIAFPKTTTAQCALTRAPSEVDPQ
Sbjct: 601 GYLLEALDMGAPPHGGIAYGVDRLVMLLADANSIRDVIAFPKTTTAQCALTRAPSEVDPQ 660

Query: 661 QLKDLSYKSQ 671
           QLKDLSY++Q
Sbjct: 661 QLKDLSYQTQ 668

BLAST of CSPI04G00050 vs. NCBI nr
Match: XP_038905135.1 (aspartate--tRNA ligase, chloroplastic/mitochondrial [Benincasa hispida])

HSP 1 Score: 1272.7 bits (3292), Expect = 0.0e+00
Identity = 637/670 (95.07%), Postives = 649/670 (96.87%), Query Frame = 0

Query: 1   MSRSLLRAFPFSTIRPKPPQFLPIRHHISHLRFPQSISTTHRPVSSFSTSATPSPSSATE 60
           MSRSLLRAFPFSTIRPKPPQFLPI HH SHLR  +SIS THRPVSSFS SA+  PSSATE
Sbjct: 1   MSRSLLRAFPFSTIRPKPPQFLPIFHHFSHLRLAKSISRTHRPVSSFSASASHPPSSATE 60

Query: 61  ILVPTSNPHSPFKDSLQWINRTALCGELSVDDVGKRVRLCGWVALHRVHGGLTFLNLRDH 120
            LVPTSN  SPFKDSLQWINRTA CGELSVDDVGKRVRLCGWVALHRVHGGLTFLNLRDH
Sbjct: 61  TLVPTSNSQSPFKDSLQWINRTAFCGELSVDDVGKRVRLCGWVALHRVHGGLTFLNLRDH 120

Query: 121 SGIVQVTTLPNEFPDAHSTINDLRLEYVVAVEGTVRSRPGDSVNVKMKTGLIEVAAEHVQ 180
           SGIVQVTTLPNEFPDAHSTINDLRLEYVVAVEGTVRSRPGDSVNVKMKTGLIEVAAEHVQ
Sbjct: 121 SGIVQVTTLPNEFPDAHSTINDLRLEYVVAVEGTVRSRPGDSVNVKMKTGLIEVAAEHVQ 180

Query: 181 ILNSVGSKLPFLVTTADDAKDSAKEEIRLRYRYLDLRRKQMNFNIMLRHKVVKLLRRYLE 240
           ILNSVGSKLPFLVTTADDAKDSAKEEIRLRYRYLDLRRKQMNFNIMLRHKVVKL+RRYLE
Sbjct: 181 ILNSVGSKLPFLVTTADDAKDSAKEEIRLRYRYLDLRRKQMNFNIMLRHKVVKLIRRYLE 240

Query: 241 DVHGFVEIETPILSRSTPEGARDYLVPSRLQPGTFFALPQSPQLFKQMLMVSGFDKYYQI 300
           DVHGFVEIETPILSRSTPEGARDYLVPSR+QPGTFFALPQSPQLFKQMLMVSGFDKYYQI
Sbjct: 241 DVHGFVEIETPILSRSTPEGARDYLVPSRVQPGTFFALPQSPQLFKQMLMVSGFDKYYQI 300

Query: 301 ARCFRDEDLRADRQPEFTQLDMELSFTTLEDMLRLNEDLIRKVFWEVKGVQLPNPFPRLT 360
           ARCFRDEDLRADRQPEFTQLDMEL+FTTLEDMLRLNEDLIRKVFWEVKGVQLPNPFPRLT
Sbjct: 301 ARCFRDEDLRADRQPEFTQLDMELAFTTLEDMLRLNEDLIRKVFWEVKGVQLPNPFPRLT 360

Query: 361 YAEAMSRYGSDRPDTRFDLQLNDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSN 420
           YAEAMSRYGSDRPDTRFDLQLNDVSDIFADSPFKVFADALSSGGIIKVLCVPSGA+RYSN
Sbjct: 361 YAEAMSRYGSDRPDTRFDLQLNDVSDIFADSPFKVFADALSSGGIIKVLCVPSGARRYSN 420

Query: 421 TALKKGDIYTEATKSGAKGLPFLKVTDDGGIEGIPALVSCLDPTDREEFLNRCSAGPGDL 480
           TALKKGDIYTEA KSGAKGLPFLKV DDGGIEGIPALVS LD   REEFL+RCSA PGDL
Sbjct: 421 TALKKGDIYTEAIKSGAKGLPFLKVMDDGGIEGIPALVSSLDSAGREEFLSRCSAEPGDL 480

Query: 481 ILFAVGHRVSVNKTLDRLRLYVANELGLVDHSRHSILWVTDFPMFEWNDLDQRLEALHHP 540
           +LFAVGHRVSV+KTLDRLRLYVANELGLVDHSRHSILWVTDFPMFEWNDL+QRLEALHHP
Sbjct: 481 VLFAVGHRVSVDKTLDRLRLYVANELGLVDHSRHSILWVTDFPMFEWNDLEQRLEALHHP 540

Query: 541 FTAPNPEDVGDLPSARALAYDMVYNGVEIGGGSLRIHKREVQEKVLEIVGISLEQAEAKF 600
           FTAPNPEDVGDLPSARALAYDMVYNGVEIGGGSLRIHKREVQEKVLEIVGIS EQAEAKF
Sbjct: 541 FTAPNPEDVGDLPSARALAYDMVYNGVEIGGGSLRIHKREVQEKVLEIVGISPEQAEAKF 600

Query: 601 GYLLEALDMGAPPHGGIAYGLDRLVMLLADANSIRDVIAFPKTTTAQCALTRAPSEVDPQ 660
           GYLLEALDMGAPPHGGIAYGLDRLVMLLADANSIRDVIAFPKT+TAQCALTRAPSEVDPQ
Sbjct: 601 GYLLEALDMGAPPHGGIAYGLDRLVMLLADANSIRDVIAFPKTSTAQCALTRAPSEVDPQ 660

Query: 661 QLKDLSYKSQ 671
           QLKDLSY +Q
Sbjct: 661 QLKDLSYHNQ 670

BLAST of CSPI04G00050 vs. NCBI nr
Match: XP_023551993.1 (aspartate--tRNA ligase, chloroplastic/mitochondrial-like [Cucurbita pepo subsp. pepo] >XP_023551994.1 aspartate--tRNA ligase, chloroplastic/mitochondrial-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1229.2 bits (3179), Expect = 0.0e+00
Identity = 613/670 (91.49%), Postives = 638/670 (95.22%), Query Frame = 0

Query: 1   MSRSLLRAFPFSTIRPKPPQFLPIRHHISHLRFPQSISTTHRPVSSFSTSATPSPSSATE 60
           MSRSLLRAFPFST+RPKPPQFLPI HHISHLR  +SIS T R VSS S       +SA E
Sbjct: 1   MSRSLLRAFPFSTLRPKPPQFLPIFHHISHLRLVKSISRTRRSVSSLS-------ASAAE 60

Query: 61  ILVPTSNPHSPFKDSLQWINRTALCGELSVDDVGKRVRLCGWVALHRVHGGLTFLNLRDH 120
            LVP+SN   P KDSLQWINRTALCGELSVDDVGKRVRLCGWVALHRVHGGLTFLNLRDH
Sbjct: 61  TLVPSSNSQLPLKDSLQWINRTALCGELSVDDVGKRVRLCGWVALHRVHGGLTFLNLRDH 120

Query: 121 SGIVQVTTLPNEFPDAHSTINDLRLEYVVAVEGTVRSRPGDSVNVKMKTGLIEVAAEHVQ 180
           SGIVQVTTLPNEFPDAHSTINDLRLEYVVAVEGTVRSRPGDSVNV MKTGLIEVAAEHVQ
Sbjct: 121 SGIVQVTTLPNEFPDAHSTINDLRLEYVVAVEGTVRSRPGDSVNVNMKTGLIEVAAEHVQ 180

Query: 181 ILNSVGSKLPFLVTTADDAKDSAKEEIRLRYRYLDLRRKQMNFNIMLRHKVVKLLRRYLE 240
           ILNSVGSKLPFLVTTADDAKD AKEEIRLRYRYLDLRRKQMNFNIMLRHKVVKL+RRYLE
Sbjct: 181 ILNSVGSKLPFLVTTADDAKDYAKEEIRLRYRYLDLRRKQMNFNIMLRHKVVKLMRRYLE 240

Query: 241 DVHGFVEIETPILSRSTPEGARDYLVPSRLQPGTFFALPQSPQLFKQMLMVSGFDKYYQI 300
           DVHGFVEIETPILSRSTPEGARDYLVPSR+QPGTFFALPQSPQLFKQMLMVSGFDKYYQI
Sbjct: 241 DVHGFVEIETPILSRSTPEGARDYLVPSRVQPGTFFALPQSPQLFKQMLMVSGFDKYYQI 300

Query: 301 ARCFRDEDLRADRQPEFTQLDMELSFTTLEDMLRLNEDLIRKVFWEVKGVQLPNPFPRLT 360
           ARCFRDEDLRADRQPEFTQLDMEL+FTTLEDML+LNEDLIRKVFWEVKGVQLPNPFP+LT
Sbjct: 301 ARCFRDEDLRADRQPEFTQLDMELAFTTLEDMLKLNEDLIRKVFWEVKGVQLPNPFPKLT 360

Query: 361 YAEAMSRYGSDRPDTRFDLQLNDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSN 420
           Y+EAMSRYGSDRPDTRFDLQL DVSD+FADS FKVFADALS+G IIKVLCVPSGAKRYSN
Sbjct: 361 YSEAMSRYGSDRPDTRFDLQLKDVSDVFADSSFKVFADALSNGEIIKVLCVPSGAKRYSN 420

Query: 421 TALKKGDIYTEATKSGAKGLPFLKVTDDGGIEGIPALVSCLDPTDREEFLNRCSAGPGDL 480
           TALKKGDIY+EA KSGAKGLPFLK+ DDGGIEGIP+LVS LDP  REEFL+RCSAGPGDL
Sbjct: 421 TALKKGDIYSEAIKSGAKGLPFLKILDDGGIEGIPSLVSSLDPAGREEFLSRCSAGPGDL 480

Query: 481 ILFAVGHRVSVNKTLDRLRLYVANELGLVDHSRHSILWVTDFPMFEWNDLDQRLEALHHP 540
           +LFAVGH+VSVNKTLDRLRLYVANELGLVDHSRHSILWVTDFPMFEWNDL+QRLEALHHP
Sbjct: 481 VLFAVGHQVSVNKTLDRLRLYVANELGLVDHSRHSILWVTDFPMFEWNDLEQRLEALHHP 540

Query: 541 FTAPNPEDVGDLPSARALAYDMVYNGVEIGGGSLRIHKREVQEKVLEIVGISLEQAEAKF 600
           FTAPNPED+ DLPSARALAYDMVYNGVEIGGGSLRIHKREVQEKVLEIVGIS EQAEAKF
Sbjct: 541 FTAPNPEDIDDLPSARALAYDMVYNGVEIGGGSLRIHKREVQEKVLEIVGISHEQAEAKF 600

Query: 601 GYLLEALDMGAPPHGGIAYGLDRLVMLLADANSIRDVIAFPKTTTAQCALTRAPSEVDPQ 660
           GYLLEALDMGAPPHGGIAYGLDRLVMLLA+ANSIRDVIAFPKTTTAQCALT+APSEV+PQ
Sbjct: 601 GYLLEALDMGAPPHGGIAYGLDRLVMLLAEANSIRDVIAFPKTTTAQCALTKAPSEVEPQ 660

Query: 661 QLKDLSYKSQ 671
           QLKDLSY++Q
Sbjct: 661 QLKDLSYQNQ 663

BLAST of CSPI04G00050 vs. TAIR 10
Match: AT4G33760.1 (tRNA synthetase class II (D, K and N) family protein )

HSP 1 Score: 979.9 bits (2532), Expect = 1.0e-285
Identity = 490/667 (73.46%), Postives = 567/667 (85.01%), Query Frame = 0

Query: 5   LLRAFPFSTIRPKPPQFLPIRHHISHLRFPQSISTTHRPVSSFSTSATPSPSSATEILV- 64
           LLR  P    R      + I +  +    P+   T   P +  + S++P  +++ +++V 
Sbjct: 4   LLRTLPLRPTRFLSATAISISNATNFFVVPK--RTNPLPGTRRTFSSSPVAAASGDVVVK 63

Query: 65  PTSNPHSPFKDSLQWINRTALCGELSVDDVGKRVRLCGWVALHRVHGGLTFLNLRDHSGI 124
           P  +P S     L+W++RT LCGELSV+DVGKRV LCGWVALHRVHGGLTFLNLRDH+GI
Sbjct: 64  PVPSPPS----VLRWVSRTELCGELSVNDVGKRVHLCGWVALHRVHGGLTFLNLRDHTGI 123

Query: 125 VQVTTLPNEFPDAHSTINDLRLEYVVAVEGTVRSRPGDSVNVKMKTGLIEVAAEHVQILN 184
           VQV TLP+EFP+AH  IND+RLEYVV VEGTVRSRP +SVN KMKTG +EV AEHV+ILN
Sbjct: 124 VQVRTLPDEFPEAHGLINDMRLEYVVLVEGTVRSRPNESVNKKMKTGFVEVVAEHVEILN 183

Query: 185 SVGSKLPFLVTTADDAKDSAKEEIRLRYRYLDLRRKQMNFNIMLRHKVVKLLRRYLEDVH 244
            V +KLPFLVTTAD+ KD  KEEIRLR+R LDLRR+QM  NI+LRH VVKL+RRYLED H
Sbjct: 184 PVRTKLPFLVTTADENKDLIKEEIRLRFRCLDLRRQQMKNNIVLRHNVVKLIRRYLEDRH 243

Query: 245 GFVEIETPILSRSTPEGARDYLVPSRLQPGTFFALPQSPQLFKQMLMVSGFDKYYQIARC 304
           GF+EIETPILSRSTPEGARDYLVPSR+Q GTF+ALPQSPQLFKQMLMVSGFDKYYQIARC
Sbjct: 244 GFIEIETPILSRSTPEGARDYLVPSRIQSGTFYALPQSPQLFKQMLMVSGFDKYYQIARC 303

Query: 305 FRDEDLRADRQPEFTQLDMELSFTTLEDMLRLNEDLIRKVFWEVKGVQLPNPFPRLTYAE 364
           FRDEDLRADRQPEFTQLDME++F  +EDML+LNEDLIRKVF E+KG+QLP+PFPRLTYA+
Sbjct: 304 FRDEDLRADRQPEFTQLDMEMAFMPMEDMLKLNEDLIRKVFSEIKGIQLPDPFPRLTYAD 363

Query: 365 AMSRYGSDRPDTRFDLQLNDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSNTAL 424
           AM RYGSDRPDTRFDL+L DVS++F +S F+VF +AL SGGIIKVLCVP GAK+YSN+AL
Sbjct: 364 AMDRYGSDRPDTRFDLELKDVSNVFTESSFRVFTEALESGGIIKVLCVPLGAKKYSNSAL 423

Query: 425 KKGDIYTEATKSGAKGLPFLKVTDDGGIEGIPALVSCLDPTDREEFLNRCSAGPGDLILF 484
           KKGDIY EA KSGAKGLPFLKV D+G IEGI ALVS LD   +  F+ +C A PGDLILF
Sbjct: 424 KKGDIYNEAMKSGAKGLPFLKVLDNGEIEGIAALVSSLDSAGKINFVKQCGAAPGDLILF 483

Query: 485 AVGHRVSVNKTLDRLRLYVANELGLVDHSRHSILWVTDFPMFEWNDLDQRLEALHHPFTA 544
            VG   SVNKTLDRLRL+VA+++ L+DHS+HSILWVTDFPMFEWN+ +QRLEALHHPFTA
Sbjct: 484 GVGPVTSVNKTLDRLRLFVAHDMDLIDHSKHSILWVTDFPMFEWNEPEQRLEALHHPFTA 543

Query: 545 PNPEDVGDLPSARALAYDMVYNGVEIGGGSLRIHKREVQEKVLEIVGISLEQAEAKFGYL 604
           P PED+ DLPSARALAYDMVYNGVEIGGGSLRI+KR+VQEKVLEI+GIS E+AE+KFGYL
Sbjct: 544 PKPEDMDDLPSARALAYDMVYNGVEIGGGSLRIYKRDVQEKVLEIIGISPEEAESKFGYL 603

Query: 605 LEALDMGAPPHGGIAYGLDRLVMLLADANSIRDVIAFPKTTTAQCALTRAPSEVDPQQLK 664
           LEALDMGAPPHGGIAYGLDR+VM+L  A+SIRDVIAFPKTTTAQCALTR PSEVDP+QL+
Sbjct: 604 LEALDMGAPPHGGIAYGLDRMVMMLGGASSIRDVIAFPKTTTAQCALTRTPSEVDPKQLQ 663

Query: 665 DLSYKSQ 671
           DLS +++
Sbjct: 664 DLSIRTK 664

BLAST of CSPI04G00050 vs. TAIR 10
Match: AT3G13490.1 (Lysyl-tRNA synthetase, class II )

HSP 1 Score: 75.1 bits (183), Expect = 2.4e-13
Identity = 69/277 (24.91%), Postives = 119/277 (42.96%), Query Frame = 0

Query: 74  DSLQWINRTALCGELSVDDVGKRVRLCGWVALHRVHGGLTFLNLRDHSGIVQVTTLPNEF 133
           + LQ I +    GE S +++   V + G V   R  G L FL LRD SG +Q+       
Sbjct: 114 NQLQEIYKHLANGEESDNEI-DCVSIAGRVVARRAFGKLAFLTLRDDSGTIQLYCEKERL 173

Query: 134 PDAHSTINDLRLEYVVAVEGTVRSRPGDSVNVKMKTGLIEVAAEHVQILNSVGSKLPFLV 193
            D        +L+  + + G +    G     + + G + +      IL      LP   
Sbjct: 174 SDDQFE----QLKQFIDI-GDILGASGSM--KRTEKGELSICVNSFSILTKSLLPLP--- 233

Query: 194 TTADDAKDSAKEEIRLRYRYLDLRRKQMNFNIMLRH-KVVKLLRRYLEDVHGFVEIETPI 253
              D        + R R RY+D+       ++  R  K+V  +R+ +E   G++E+ETP+
Sbjct: 234 ---DKYHGLTDIDKRYRQRYVDMIANPEVADVFRRRAKIVSEIRKTVES-FGYLEVETPV 293

Query: 254 LSRSTPEGARDYLVPSRLQPGTFFALPQSPQLFKQMLMVSGFDKYYQIARCFRDEDLRAD 313
           L  +         V      G    L  + +L  + ++V GF+K Y+I R FR+E +   
Sbjct: 294 LQGAAGGAEARPFVTFHNSLGRDLYLRIATELHLKRMLVGGFEKVYEIGRIFRNEGISTR 353

Query: 314 RQPEFTQLDMELSFTTLEDMLRLNEDLIRKVFWEVKG 350
             PEFT ++M  +++    M+ + E ++ +    V G
Sbjct: 354 HNPEFTTIEMYEAYSDYHSMMDMAELIVTQCSMAVNG 375


HSP 2 Score: 50.8 bits (120), Expect = 4.8e-06
Identity = 24/38 (63.16%), Postives = 29/38 (76.32%), Query Frame = 0

Query: 604 LEALDMGAPPHGGIAYGLDRLVMLLADANSIRDVIAFP 642
           L AL+ G PP  G+  G+DRLVMLL ++ SIRDVIAFP
Sbjct: 559 LTALEYGMPPASGMGLGIDRLVMLLTNSASIRDVIAFP 596

BLAST of CSPI04G00050 vs. TAIR 10
Match: AT3G11710.1 (lysyl-tRNA synthetase 1 )

HSP 1 Score: 61.2 bits (147), Expect = 3.6e-09
Identity = 38/146 (26.03%), Postives = 74/146 (50.68%), Query Frame = 0

Query: 205 EEIRLRYRYLD-LRRKQMNFNIMLRHKVVKLLRRYLEDVHGFVEIETPILSRSTPEGARD 264
           +E R R R+LD +   ++      R K++  +RR+L D   F+E+ETP+++      A  
Sbjct: 259 QESRYRQRHLDMILNVEVRQIFRTRAKIISYVRRFL-DNKNFLEVETPMMNMIAGGAAAR 318

Query: 265 YLVPSRLQPGTFFALPQSPQLFKQMLMVSGFDKYYQIARCFRDEDLRADRQPEFTQLDME 324
             V           +  +P+L+ + L+V G ++ Y+I + FR+E +     PEFT  +  
Sbjct: 319 PFVTHHNDLDMRLYMRIAPELYLKQLIVGGLERVYEIGKQFRNEGIDLTHNPEFTTCEFY 378

Query: 325 LSFTTLEDMLRLNEDLIRKVFWEVKG 350
           ++F    D++ + E ++  +  E+ G
Sbjct: 379 MAFADYNDLMEMTEVMLSGMVKELTG 403


HSP 2 Score: 39.7 bits (91), Expect = 1.1e-02
Identity = 21/51 (41.18%), Postives = 31/51 (60.78%), Query Frame = 0

Query: 606 ALDMGAPPHGGIAYGLDRLVMLLADANSIRDVIAFP--KTTTAQCALTRAP 655
           AL+ G  P GG   G+DRL MLL D+ +I++V+ FP  +    + A  +AP
Sbjct: 570 ALEYGLAPTGGWGLGIDRLSMLLTDSLNIKEVLFFPAMRPPQEESAAAQAP 620

BLAST of CSPI04G00050 vs. TAIR 10
Match: AT4G26870.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 60.8 bits (146), Expect = 4.7e-09
Identity = 33/84 (39.29%), Postives = 46/84 (54.76%), Query Frame = 0

Query: 559 AYDMVYNGVEIGGGSLRIHKREVQEKVLEIVGISLEQAEAKFGYLLEALDMGAPPHGGIA 618
           ++D+   G EI  G+ RIH  E+ EK     GI ++         ++A   GAPPHGG  
Sbjct: 446 SFDVFIRGEEIMSGAQRIHDPELLEKRARECGIDVKTIST----YIDAFRYGAPPHGGFG 505

Query: 619 YGLDRLVMLLADANSIRDVIAFPK 643
            GL+R+VMLL   N+IR    FP+
Sbjct: 506 VGLERVVMLLCALNNIRKTSLFPR 525

BLAST of CSPI04G00050 vs. TAIR 10
Match: AT4G31180.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 51.6 bits (122), Expect = 2.8e-06
Identity = 29/84 (34.52%), Postives = 44/84 (52.38%), Query Frame = 0

Query: 559 AYDMVYNGVEIGGGSLRIHKREVQEKVLEIVGISLEQAEAKFGYLLEALDMGAPPHGGIA 618
           ++D+   G EI  G+ R+H  EV E+     GI ++         +++   GAP HGG  
Sbjct: 472 SFDVFIRGEEIISGAQRVHIPEVLEQRAGECGIDVKTIST----YIDSFRYGAPLHGGFG 531

Query: 619 YGLDRLVMLLADANSIRDVIAFPK 643
            GL+R+VML    N+IR    FP+
Sbjct: 532 VGLERVVMLFCALNNIRKTSLFPR 551

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F4JJT91.4e-28473.46Aspartate--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3... [more]
Q112K01.0e-21059.83Aspartate--tRNA(Asp/Asn) ligase OS=Trichodesmium erythraeum (strain IMS101) OX=2... [more]
Q8YUB68.7e-21060.34Aspartate--tRNA(Asp/Asn) ligase OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTE... [more]
Q3MGL33.3e-20960.17Aspartate--tRNA(Asp/Asn) ligase OS=Trichormus variabilis (strain ATCC 29413 / PC... [more]
B2J1161.3e-20558.31Aspartate--tRNA(Asp/Asn) ligase OS=Nostoc punctiforme (strain ATCC 29133 / PCC 7... [more]
Match NameE-valueIdentityDescription
A0A0A0KT150.0e+0099.70AA_TRNA_LIGASE_II domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G... [more]
A0A5D3E1M90.0e+0097.01Aspartate--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold6... [more]
A0A1S3BZT50.0e+0097.01aspartate--tRNA ligase, chloroplastic/mitochondrial isoform X1 OS=Cucumis melo O... [more]
A0A5A7TRV50.0e+0096.57Aspartate--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold4... [more]
A0A6J1D6F80.0e+0091.51aspartate--tRNA ligase, chloroplastic/mitochondrial OS=Momordica charantia OX=36... [more]
Match NameE-valueIdentityDescription
XP_004150278.20.0e+0099.70aspartate--tRNA ligase, chloroplastic/mitochondrial [Cucumis sativus] >KGN52733.... [more]
XP_008454426.10.0e+0097.01PREDICTED: aspartate--tRNA ligase, chloroplastic/mitochondrial isoform X1 [Cucum... [more]
KAA0044311.10.0e+0096.57aspartate--tRNA ligase [Cucumis melo var. makuwa][more]
XP_038905135.10.0e+0095.07aspartate--tRNA ligase, chloroplastic/mitochondrial [Benincasa hispida][more]
XP_023551993.10.0e+0091.49aspartate--tRNA ligase, chloroplastic/mitochondrial-like [Cucurbita pepo subsp. ... [more]
Match NameE-valueIdentityDescription
AT4G33760.11.0e-28573.46tRNA synthetase class II (D, K and N) family protein [more]
AT3G13490.12.4e-1324.91Lysyl-tRNA synthetase, class II [more]
AT3G11710.13.6e-0926.03lysyl-tRNA synthetase 1 [more]
AT4G26870.14.7e-0939.29Class II aminoacyl-tRNA and biotin synthetases superfamily protein [more]
AT4G31180.12.8e-0634.52Class II aminoacyl-tRNA and biotin synthetases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002312Aspartyl/Asparaginyl-tRNA synthetase, class IIbPRINTSPR01042TRNASYNTHASPcoord: 278..290
score: 65.38
coord: 560..576
score: 55.21
coord: 604..618
score: 51.06
coord: 295..308
score: 54.06
NoneNo IPR availableGENE3D3.30.930.10Bira Bifunctional Protein; Domain 2coord: 199..665
e-value: 1.5E-186
score: 622.6
NoneNo IPR availableGENE3D2.40.50.140coord: 73..185
e-value: 4.2E-32
score: 112.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 42..64
NoneNo IPR availablePANTHERPTHR22594ASPARTYL/LYSYL-TRNA SYNTHETASEcoord: 58..668
NoneNo IPR availableCDDcd04317EcAspRS_like_Ncoord: 81..221
e-value: 9.14289E-59
score: 192.351
NoneNo IPR availableCDDcd00777AspRS_corecoord: 225..644
e-value: 3.92324E-157
score: 452.029
NoneNo IPR availableSUPERFAMILY55681Class II aaRS and biotin synthetasescoord: 205..643
IPR004115GAD-like domain superfamilyGENE3D3.30.1360.30coord: 360..509
e-value: 1.5E-186
score: 622.6
IPR004115GAD-like domain superfamilySUPERFAMILY55261GAD domain-likecoord: 377..509
IPR004364Aminoacyl-tRNA synthetase, class II (D/K/N)PFAMPF00152tRNA-synt_2coord: 205..644
e-value: 1.0E-107
score: 360.1
IPR004524Aspartate-tRNA ligase, type 1TIGRFAMTIGR00459TIGR00459coord: 80..668
e-value: 1.8E-221
score: 734.5
IPR004524Aspartate-tRNA ligase, type 1PANTHERPTHR22594:SF5ASPARTATE--TRNA LIGASE, MITOCHONDRIALcoord: 58..668
IPR004524Aspartate-tRNA ligase, type 1HAMAPMF_00044Asp_tRNA_synth_type1coord: 80..670
score: 32.866776
IPR029351GAD domainPFAMPF02938GADcoord: 396..495
e-value: 6.2E-20
score: 71.2
IPR004365OB-fold nucleic acid binding domain, AA-tRNA synthetase-typePFAMPF01336tRNA_anti-codoncoord: 97..179
e-value: 4.3E-12
score: 45.9
IPR006195Aminoacyl-tRNA synthetase, class IIPROSITEPS50862AA_TRNA_LIGASE_IIcoord: 227..641
score: 20.965586
IPR012340Nucleic acid-binding, OB-foldSUPERFAMILY50249Nucleic acid-binding proteinscoord: 81..183

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI04G00050.1CSPI04G00050.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006422 aspartyl-tRNA aminoacylation
biological_process GO:0006418 tRNA aminoacylation for protein translation
cellular_component GO:0005739 mitochondrion
cellular_component GO:0005737 cytoplasm
molecular_function GO:0004815 aspartate-tRNA ligase activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0003677 DNA binding
molecular_function GO:0004812 aminoacyl-tRNA ligase activity
molecular_function GO:0016874 ligase activity
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0000166 nucleotide binding