CSPI03G47650 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI03G47650
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	heavy metal-associated isoprenylated plant protein 31
Location	Chr3: 40626179 .. 40628041 (+)
RNA-Seq Expression	CSPI03G47650
Synteny	CSPI03G47650

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TGCCATTTATGCATGCAACAAAGTTAATAGCCTAATTAAACAACACTCTCACTTTTACTCTCTCTTATATTTTTTTTTTGTTCAAACAAAAGCATTCAACAATGTCTGTAAGTTTCTCTCGCTCTTCTCTCTTAAACAACTAGCACACCATAATCACTCTATTACATCTTTCTCTCCTTCTTCTTTAATGCATGTCTCTCATTGTGTATTGCTATATATTTGTATATAGATGGTGGAGGTTAGAGTTCCAAATCTCGACTGTGAGGGGTGCGCTTCGAAGTTAAAGAAAGCTCTTTTCAAGCTTAAAGGTACATCATTCATCATTTGAGTATACACACAACGTTTTTGTTTCTTGTTTTAATCTATGTTTAGGTAATTAACATTTTTTGATATAATGGTTCTTGTTTATAGTTTTTTCTTTTGAACATAATTAAAAAAGTATTGTTTTATGATTCTTATCTAATGAAATGTAGAATATTGTAATAAATATTAAATGTCCTGTTTTAAAATCTGTTGTTTCTAAATTCATTTTTTTAAATCTAAATTTTGTGTTTGACTTGTAAGGCCACATTTGAAAATCCTTCTTATTTTTTAGTTTAGATATTAGGCTTTATTCCTTTTTTACTCTCTCTGTTTTCTTTTAAGAAACAAACACCATGCTGTTAGTCTAATTTCAAAAACACTAAACAAATTTTGAAAGAACTGGTTTGGATTCTGAAAACATACCTAGAAAAGTATATAGAAAAATCAATGGGTGAAGTAGCCAGTGTTATTTTAAAAATTAAGTGTACTTTTATCAGGTTTAAAAAATCACTCATAAACTTACCTTTGATCACCTAAAATTAGTTTAATATTGAATTTGAAACTTAAGAAAGTAAATTTCATGTCTATGATATCTAATTGTTGTAATGCAAAAGAAAAAAGGAAAGAAGAAAAGAAAAAAGTGTGTGAGGGGTGCAGCTTGTGTTGGTTGGGCAGAGCAAGGGGTGGGAGACAGTGGTCCTTTTTTATTCATTCTCTATTTAAAAAGTGACAGTTCATTTGTCCCCAAAAAGTGAAAACCATTTTTAAAGATATGCAATATAAACTTTCATACACCACACTACCAAGTGTGGATATATATACATGTGTATATATGGAGAGAGGTGAAGAGAGAAAATTGGATTATTATTATTAACAATGAAGGGAATATATATTTTGTAGGAGTTGAAGAAGTGGAGGTGGAAATAGAGATGCAAAAAATAACAGTAAGAGGGTATGGATTGGAAGAGAGAAAAGTGGTAAAGGCAATAAAGAGAGCTGGAAAAGCAGCAGAAGGATGGCCATTTCCAGGATATTCATCTCATTACACTTCCTTCTATAAATACCCTTCTTATATAGCCAACCATTATTATGACACCTACGGGGGCCACAATTCCAATTCCAATTCCAATTCCAACTATTCTACTACTACTACTTCCTCAAATAAACATCATCATCATCATCATCATCTCAATATTTCTAATTCAAATAATTGTTCTTCCCAGCTTCACACTTTCTTTCAAACTCCTTCCCTTTACTCTCTTGCTCTCTCCTCTGACCATGCCATTGCTTCCCTTTTTAGTGATGACAATCCTCATGCTTGTTCTATCATGTGATTTCTCTCTCTCATCTCTACTACTCATCATCATCCCCAATTCCTTTTCCCATCATCTCCTTTTACTTCTTTTGTAATTTATTATTTCACTTTCTTACTTTACTATTATTCTATTTCTCTTCACTTTCAAATTCCAAGTTTAATTTATGTATCTATTCTTCGCCCAGGATTATATTCATTGTGTTTGTTTGTGTTCTTAATTACTTGGTTTAATGGGACTTTTCTTT

mRNA sequence

TGCCATTTATGCATGCAACAAAGTTAATAGCCTAATTAAACAACACTCTCACTTTTACTCTCTCTTATATTTTTTTTTTGTTCAAACAAAAGCATTCAACAATGTCTATGGTGGAGGTTAGAGTTCCAAATCTCGACTGTGAGGGGTGCGCTTCGAAGTTAAAGAAAGCTCTTTTCAAGCTTAAAGGAGTTGAAGAAGTGGAGGTGGAAATAGAGATGCAAAAAATAACAGTAAGAGGGTATGGATTGGAAGAGAGAAAAGTGGTAAAGGCAATAAAGAGAGCTGGAAAAGCAGCAGAAGGATGGCCATTTCCAGGATATTCATCTCATTACACTTCCTTCTATAAATACCCTTCTTATATAGCCAACCATTATTATGACACCTACGGGGGCCACAATTCCAATTCCAATTCCAATTCCAACTATTCTACTACTACTACTTCCTCAAATAAACATCATCATCATCATCATCATCTCAATATTTCTAATTCAAATAATTGTTCTTCCCAGCTTCACACTTTCTTTCAAACTCCTTCCCTTTACTCTCTTGCTCTCTCCTCTGACCATGCCATTGCTTCCCTTTTTAGTGATGACAATCCTCATGCTTGTTCTATCATGTGATTTCTCTCTCTCATCTCTACTACTCATCATCATCCCCAATTCCTTTTCCCATCATCTCCTTTTACTTCTTTTGTAATTTATTATTTCACTTTCTTACTTTACTATTATTCTATTTCTCTTCACTTTCAAATTCCAAGTTTAATTTATGTATCTATTCTTCGCCCAGGATTATATTCATTGTGTTTGTTTGTGTTCTTAATTACTTGGTTTAATGGGACTTTTCTTT

Coding sequence (CDS)

ATGTCTATGGTGGAGGTTAGAGTTCCAAATCTCGACTGTGAGGGGTGCGCTTCGAAGTTAAAGAAAGCTCTTTTCAAGCTTAAAGGAGTTGAAGAAGTGGAGGTGGAAATAGAGATGCAAAAAATAACAGTAAGAGGGTATGGATTGGAAGAGAGAAAAGTGGTAAAGGCAATAAAGAGAGCTGGAAAAGCAGCAGAAGGATGGCCATTTCCAGGATATTCATCTCATTACACTTCCTTCTATAAATACCCTTCTTATATAGCCAACCATTATTATGACACCTACGGGGGCCACAATTCCAATTCCAATTCCAATTCCAACTATTCTACTACTACTACTTCCTCAAATAAACATCATCATCATCATCATCATCTCAATATTTCTAATTCAAATAATTGTTCTTCCCAGCTTCACACTTTCTTTCAAACTCCTTCCCTTTACTCTCTTGCTCTCTCCTCTGACCATGCCATTGCTTCCCTTTTTAGTGATGACAATCCTCATGCTTGTTCTATCATGTGA

Protein sequence

MSMVEVRVPNLDCEGCASKLKKALFKLKGVEEVEVEIEMQKITVRGYGLEERKVVKAIKRAGKAAEGWPFPGYSSHYTSFYKYPSYIANHYYDTYGGHNSNSNSNSNYSTTTTSSNKHHHHHHHLNISNSNNCSSQLHTFFQTPSLYSLALSSDHAIASLFSDDNPHACSIM*

Homology

[Close]

BLAST of CSPI03G47650 vs. ExPASy Swiss-Prot
Match: Q84K70 (Heavy metal-associated isoprenylated plant protein 31 OS=Arabidopsis thaliana OX=3702 GN=HIPP31 PE=2 SV=1)

HSP 1 Score: 169.1 bits (427), Expect = 4.4e-41
Identity = 94/173 (54.34%), Postives = 116/173 (67.05%), Query Frame = 0


Query: 1   MSM-VEVRVPNLDCEGCASKLKKALFKLKGVEEVEVEIEMQKITVRGYGLEERKVVKAIK 60
           MSM VE+RVPNLDCEGCASKL+K L KLKGVEEVEVE+E QK+T RGY LEE+KV+KA++
Sbjct: 1   MSMTVEIRVPNLDCEGCASKLRKTLLKLKGVEEVEVEMETQKVTARGYRLEEKKVLKAVR 60

Query: 61  RAGKAAEGWPFPGYSSHYTSFYKYPSYIANHYYDTYGGHNSNSNSNSNYSTTTTSSNKHH 120
           RAGKAAE WP+   +SH+ SFYKYPSY+ NHYY                      S+ H 
Sbjct: 61  RAGKAAELWPYRLGNSHFASFYKYPSYVTNHYY----------------------SDAHR 120

Query: 121 HHHHHLNISNSNNCSSQLHTFFQTPSLYSLALSSDHAIASLFSDDNPHACSIM 173
                       + +  +HTFF TP+ YS+A++ D   AS+FSDDNPHAC+IM
Sbjct: 121 -----------TDPTGGVHTFFHTPADYSVAVAGDEIAASMFSDDNPHACTIM 140

[Close]

BLAST of CSPI03G47650 vs. ExPASy Swiss-Prot
Match: B3H6D0 (Heavy metal-associated isoprenylated plant protein 45 OS=Arabidopsis thaliana OX=3702 GN=HIPP45 PE=3 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 2.1e-14
Identity = 60/172 (34.88%), Postives = 99/172 (57.56%), Query Frame = 0


Query: 1   MSMVEVRVPNLDCEGCASKLKKALFKLKGVEEVEVEIEMQKITVRGYGLEERKVVKAIKR 60
           +S+VE+ V ++DC+GC  K+++A+ KL GV+ VE++++ QK+TV GY ++  +V+K +KR
Sbjct: 15  LSIVELLV-DMDCKGCEKKVRRAISKLDGVDTVEIDVDRQKVTVTGY-VDREEVLKMVKR 74

Query: 61  AGKAAEGWPFPGYSSHYTSFYKYPSYIANHYYDTYGGHNSNSNSNSNYSTTTTSSNKHHH 120
            G+ AE WPFP Y+ +Y  +Y YPS                  S+     T + S K+  
Sbjct: 75  TGRTAEYWPFP-YNGYYGDYYTYPS-------------QHLEQSDQKIYQTISYSGKYDF 134

Query: 121 HHHHLNISNSNNCSSQLHTFFQTPSLYSLALSSDHAIASLFSDDNPHACSIM 173
           +    +  N+NN  S ++ ++ + S   +  + D     LFSDDN HAC+IM
Sbjct: 135 YDVD-DFQNTNN--STINGYYPSSS-QKVQPNIDENALHLFSDDNAHACTIM 166

[Close]

BLAST of CSPI03G47650 vs. ExPASy Swiss-Prot
Match: F4IQG4 (Heavy metal-associated isoprenylated plant protein 30 OS=Arabidopsis thaliana OX=3702 GN=HIPP30 PE=1 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 1.8e-13
Identity = 52/160 (32.50%), Postives = 86/160 (53.75%), Query Frame = 0


Query: 13  CEGCASKLKKALFKLKGVEEVEVEIEMQKITVRGYGLEERKVVKAIKRAGKAAEGWPFPG 72
           C GC   +K A++KL+GV+ VEV +EM+++TV GY +E +KV+KA++RAGK AE WP+P 
Sbjct: 56  CSGCERVVKHAIYKLRGVDSVEVNLEMERVTVVGY-VERKKVLKAVRRAGKRAEFWPYPD 115

Query: 73  YSSHYTSFYKYPSYIANHYYDTYGGHNSNSNSNSNYSTTTTSSNKHHHHHHHLNISNSNN 132
              ++TS        ++HY+                 TT      ++++ H  N+S+ + 
Sbjct: 116 MPRYFTS--------SDHYFK---------------DTTREFRESYNYYRHGYNLSDRH- 175

Query: 133 CSSQLHTFFQTPSLYSLALSSDHAIASLFSDDNPHACSIM 173
               +H          +    D  +++ F+DDN HACS+M
Sbjct: 176 --GNIH----------VTNRGDDKMSNFFNDDNVHACSLM 178

[Close]

BLAST of CSPI03G47650 vs. ExPASy Swiss-Prot
Match: F4IC29 (Heavy metal-associated isoprenylated plant protein 28 OS=Arabidopsis thaliana OX=3702 GN=HIPP28 PE=3 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 2.8e-11
Identity = 57/172 (33.14%), Postives = 96/172 (55.81%), Query Frame = 0


Query: 1   MSMVEVRVPNLDCEGCASKLKKALFKLKGVEEVEVEIEMQKITVRGYGLEERKVVKAIKR 60
           +  +E+RV ++DC GC S++K AL K++GV+ VE+++  QK+TV GY  +++KV+K +++
Sbjct: 10  LQTIEMRV-HMDCVGCESRVKNALQKMRGVDAVEIDMVQQKVTVTGYA-DQKKVLKKVRK 69

Query: 61  AGKAAEGWPFPGYSSHYTSFYKYPSYIANHYYDTYGGHNSNSNSNSNYSTTTTSSNKHHH 120
            G+ AE W  P    H        S    ++Y+  G      N   N++    +S+ +++
Sbjct: 70  TGRRAELWQLPYNPDHMGG----SSSNGGYFYNPQG-----CNGPINHAAPVPTSSYNYY 129

Query: 121 HHHHLNISNSNNCSSQLHTFFQTPSLYSLALSSDHAIASLFSDDNPHACSIM 173
            H +    +SN+ SS  H      S++S      H   S FSD+NP+ACSIM
Sbjct: 130 KHGY----DSNDYSSYRHHPVHA-SIFS------HQTGSKFSDENPNACSIM 159

[Close]

BLAST of CSPI03G47650 vs. ExPASy Swiss-Prot
Match: O65657 (Heavy metal-associated isoprenylated plant protein 23 OS=Arabidopsis thaliana OX=3702 GN=HIPP23 PE=1 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 2.9e-08
Identity = 56/169 (33.14%), Postives = 76/169 (44.97%), Query Frame = 0


Query: 4   VEVRVPNLDCEGCASKLKKALFKLKGVEEVEVEIEMQKITVRGYGLEERKVVKAIKRAGK 63
           VE++V  +DC+GC  K+K +L  LKGV+ VE+  + QK+TV GY  +  KV+K  K  GK
Sbjct: 34  VELKV-RMDCDGCVLKIKNSLSSLKGVKTVEINKKQQKVTVSGYA-DASKVLKKAKATGK 93

Query: 64  AAEGWPFPGYSSHYTSFYKYPSYIANHYYDTYGGHNSNSNSNSNYSTTTTSSNKHHHHHH 123
            AE WP+  Y+           YIA   YD           + N +T T           
Sbjct: 94  KAEIWPYVPYN------LVAQPYIA-QAYDKKAPPGYVRKVDPNVTTGT----------- 153

Query: 124 HLNISNSNNCSSQLHTFFQTPSLYSLALSSDHAIASLFSDDNPHACSIM 173
                        +  ++  PS             SLFSDDNP+ACSIM
Sbjct: 154 -------------MAVYYDDPS-----------YTSLFSDDNPNACSIM 158

[Close]

BLAST of CSPI03G47650 vs. ExPASy TrEMBL
Match: A0A0A0LHJ7 (HMA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G914050 PE=4 SV=1)

HSP 1 Score: 333.2 bits (853), Expect = 6.6e-88
Identity = 172/173 (99.42%), Postives = 172/173 (99.42%), Query Frame = 0


Query: 1   MSMVEVRVPNLDCEGCASKLKKALFKLKGVEEVEVEIEMQKITVRGYGLEERKVVKAIKR 60
           MSMVEVRVPNLDCEGCASKLKKALFKLKGVEEVEVEIEMQKITVRGYGLEERKVVKAIKR
Sbjct: 1   MSMVEVRVPNLDCEGCASKLKKALFKLKGVEEVEVEIEMQKITVRGYGLEERKVVKAIKR 60

Query: 61  AGKAAEGWPFPGYSSHYTSFYKYPSYIANHYYDTYGGHNSNSNSNSNYSTTTTSSNK-HH 120
           AGKAAEGWPFPGYSSHYTSFYKYPSYIANHYYDTYGGHNSNSNSNSNYSTTTTSSNK HH
Sbjct: 61  AGKAAEGWPFPGYSSHYTSFYKYPSYIANHYYDTYGGHNSNSNSNSNYSTTTTSSNKHHH 120

Query: 121 HHHHHLNISNSNNCSSQLHTFFQTPSLYSLALSSDHAIASLFSDDNPHACSIM 173
           HHHHHLNISNSNNCSSQLHTFFQTPSLYSLALSSDHAIASLFSDDNPHACSIM
Sbjct: 121 HHHHHLNISNSNNCSSQLHTFFQTPSLYSLALSSDHAIASLFSDDNPHACSIM 173

[Close]

BLAST of CSPI03G47650 vs. ExPASy TrEMBL
Match: A0A1S3CQ83 (heavy metal-associated isoprenylated plant protein 20 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103503458 PE=4 SV=1)

HSP 1 Score: 307.0 bits (785), Expect = 5.0e-80
Identity = 165/181 (91.16%), Postives = 169/181 (93.37%), Query Frame = 0


Query: 1   MSMVEVRVPNLDCEGCASKLKKALFKLKGVEEVEVEIEMQKITVRGYGLEERKVVKAIKR 60
           MSMVEVRVPNLDCEGCASKL+KALFKLKGVEEVEVEIEMQKITVRGYGLEERKVVKAIKR
Sbjct: 1   MSMVEVRVPNLDCEGCASKLRKALFKLKGVEEVEVEIEMQKITVRGYGLEERKVVKAIKR 60

Query: 61  AGKAAEGWPFPGYSSHYTSFYKYPSYIANHYYDTYGGHN-----SNSNSNSNYSTTTT-- 120
           AGKAAEGWPFPGYSSHYTSFYKYPS IANHYYDTYGGHN     SNSNSNSNY+T+TT  
Sbjct: 61  AGKAAEGWPFPGYSSHYTSFYKYPSSIANHYYDTYGGHNSYSYYSNSNSNSNYTTSTTTT 120

Query: 121 --SSNKHHHHHHHLNISNSNNCSSQLHTFFQTPSLYSLALSSDHAIASLFSDDNPHACSI 173
             SSNK HHHHHHLN+SNSNN SSQLHTFFQTPSLYSLALSSDHAIASLFSDDNPHACSI
Sbjct: 121 PPSSNK-HHHHHHLNLSNSNNYSSQLHTFFQTPSLYSLALSSDHAIASLFSDDNPHACSI 180

[Close]

BLAST of CSPI03G47650 vs. ExPASy TrEMBL
Match: A0A1S3CRB2 (heavy metal-associated isoprenylated plant protein 20 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103503458 PE=4 SV=1)

HSP 1 Score: 303.5 bits (776), Expect = 5.6e-79
Identity = 163/179 (91.06%), Postives = 167/179 (93.30%), Query Frame = 0


Query: 3   MVEVRVPNLDCEGCASKLKKALFKLKGVEEVEVEIEMQKITVRGYGLEERKVVKAIKRAG 62
           MVEVRVPNLDCEGCASKL+KALFKLKGVEEVEVEIEMQKITVRGYGLEERKVVKAIKRAG
Sbjct: 1   MVEVRVPNLDCEGCASKLRKALFKLKGVEEVEVEIEMQKITVRGYGLEERKVVKAIKRAG 60

Query: 63  KAAEGWPFPGYSSHYTSFYKYPSYIANHYYDTYGGHN-----SNSNSNSNYSTTTT---- 122
           KAAEGWPFPGYSSHYTSFYKYPS IANHYYDTYGGHN     SNSNSNSNY+T+TT    
Sbjct: 61  KAAEGWPFPGYSSHYTSFYKYPSSIANHYYDTYGGHNSYSYYSNSNSNSNYTTSTTTTPP 120

Query: 123 SSNKHHHHHHHLNISNSNNCSSQLHTFFQTPSLYSLALSSDHAIASLFSDDNPHACSIM 173
           SSNK HHHHHHLN+SNSNN SSQLHTFFQTPSLYSLALSSDHAIASLFSDDNPHACSIM
Sbjct: 121 SSNK-HHHHHHLNLSNSNNYSSQLHTFFQTPSLYSLALSSDHAIASLFSDDNPHACSIM 178

[Close]

BLAST of CSPI03G47650 vs. ExPASy TrEMBL
Match: A0A6J1I865 (heavy metal-associated isoprenylated plant protein 31 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111470546 PE=4 SV=1)

HSP 1 Score: 236.9 bits (603), Expect = 6.4e-59
Identity = 128/172 (74.42%), Postives = 134/172 (77.91%), Query Frame = 0


Query: 1   MSMVEVRVPNLDCEGCASKLKKALFKLKGVEEVEVEIEMQKITVRGYGLEERKVVKAIKR 60
           MSM+EVRVPNLDCEGCASKL+KALFKLKGVEEVEVEIEMQKITVRGYGLEERKVVKAIKR
Sbjct: 14  MSMIEVRVPNLDCEGCASKLRKALFKLKGVEEVEVEIEMQKITVRGYGLEERKVVKAIKR 73

Query: 61  AGKAAEGWPFPGYSSHYTSFYKYPSYIANHYYDTYGGHNSNSNSNSNYSTTTTSSNKHHH 120
           AGKAAE WPFPGYSSHY SFYKYPSYIA HYYD              YS TT  +  +HH
Sbjct: 74  AGKAAEAWPFPGYSSHYASFYKYPSYIATHYYDA-------------YSATTNPTTTNHH 133

Query: 121 HHHHLNISNSNNCSSQLHTFFQTPSLYSLALSSDHAIASLFSDDNPHACSIM 173
                         + LHTFFQTPSLYS A+SSDHAIASLFSDDNPHACSIM
Sbjct: 134 -----------ASPTSLHTFFQTPSLYSRAVSSDHAIASLFSDDNPHACSIM 161

[Close]

BLAST of CSPI03G47650 vs. ExPASy TrEMBL
Match: A0A6J1F0F2 (heavy metal-associated isoprenylated plant protein 31 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111438064 PE=4 SV=1)

HSP 1 Score: 236.1 bits (601), Expect = 1.1e-58
Identity = 128/172 (74.42%), Postives = 134/172 (77.91%), Query Frame = 0


Query: 1   MSMVEVRVPNLDCEGCASKLKKALFKLKGVEEVEVEIEMQKITVRGYGLEERKVVKAIKR 60
           MSM+EVRVPNLDCEGCASKL+KALFKLKGVEEVEVEIEMQKITVRGYGLEERKVVKAIKR
Sbjct: 14  MSMIEVRVPNLDCEGCASKLRKALFKLKGVEEVEVEIEMQKITVRGYGLEERKVVKAIKR 73

Query: 61  AGKAAEGWPFPGYSSHYTSFYKYPSYIANHYYDTYGGHNSNSNSNSNYSTTTTSSNKHHH 120
           AGKAAE WPFPGYSSHY SFYKYPSYIA HYYD              YS TT  +  +HH
Sbjct: 74  AGKAAEAWPFPGYSSHYASFYKYPSYIATHYYDA-------------YSATTNPTATNHH 133

Query: 121 HHHHLNISNSNNCSSQLHTFFQTPSLYSLALSSDHAIASLFSDDNPHACSIM 173
                         + LHTFFQTPSLYS A+SSDHAIASLFSDDNPHACSIM
Sbjct: 134 -----------ASPTSLHTFFQTPSLYSRAVSSDHAIASLFSDDNPHACSIM 161

[Close]

BLAST of CSPI03G47650 vs. NCBI nr
Match: XP_004148352.2 (heavy metal-associated isoprenylated plant protein 31 [Cucumis sativus] >KGN60474.1 hypothetical protein Csa_000961 [Cucumis sativus])

HSP 1 Score: 333.2 bits (853), Expect = 1.4e-87
Identity = 172/173 (99.42%), Postives = 172/173 (99.42%), Query Frame = 0


Query: 1   MSMVEVRVPNLDCEGCASKLKKALFKLKGVEEVEVEIEMQKITVRGYGLEERKVVKAIKR 60
           MSMVEVRVPNLDCEGCASKLKKALFKLKGVEEVEVEIEMQKITVRGYGLEERKVVKAIKR
Sbjct: 1   MSMVEVRVPNLDCEGCASKLKKALFKLKGVEEVEVEIEMQKITVRGYGLEERKVVKAIKR 60

Query: 61  AGKAAEGWPFPGYSSHYTSFYKYPSYIANHYYDTYGGHNSNSNSNSNYSTTTTSSNK-HH 120
           AGKAAEGWPFPGYSSHYTSFYKYPSYIANHYYDTYGGHNSNSNSNSNYSTTTTSSNK HH
Sbjct: 61  AGKAAEGWPFPGYSSHYTSFYKYPSYIANHYYDTYGGHNSNSNSNSNYSTTTTSSNKHHH 120

Query: 121 HHHHHLNISNSNNCSSQLHTFFQTPSLYSLALSSDHAIASLFSDDNPHACSIM 173
           HHHHHLNISNSNNCSSQLHTFFQTPSLYSLALSSDHAIASLFSDDNPHACSIM
Sbjct: 121 HHHHHLNISNSNNCSSQLHTFFQTPSLYSLALSSDHAIASLFSDDNPHACSIM 173

[Close]

BLAST of CSPI03G47650 vs. NCBI nr
Match: XP_008465869.1 (PREDICTED: heavy metal-associated isoprenylated plant protein 20 isoform X1 [Cucumis melo])

HSP 1 Score: 307.0 bits (785), Expect = 1.0e-79
Identity = 165/181 (91.16%), Postives = 169/181 (93.37%), Query Frame = 0


Query: 1   MSMVEVRVPNLDCEGCASKLKKALFKLKGVEEVEVEIEMQKITVRGYGLEERKVVKAIKR 60
           MSMVEVRVPNLDCEGCASKL+KALFKLKGVEEVEVEIEMQKITVRGYGLEERKVVKAIKR
Sbjct: 1   MSMVEVRVPNLDCEGCASKLRKALFKLKGVEEVEVEIEMQKITVRGYGLEERKVVKAIKR 60

Query: 61  AGKAAEGWPFPGYSSHYTSFYKYPSYIANHYYDTYGGHN-----SNSNSNSNYSTTTT-- 120
           AGKAAEGWPFPGYSSHYTSFYKYPS IANHYYDTYGGHN     SNSNSNSNY+T+TT  
Sbjct: 61  AGKAAEGWPFPGYSSHYTSFYKYPSSIANHYYDTYGGHNSYSYYSNSNSNSNYTTSTTTT 120

Query: 121 --SSNKHHHHHHHLNISNSNNCSSQLHTFFQTPSLYSLALSSDHAIASLFSDDNPHACSI 173
             SSNK HHHHHHLN+SNSNN SSQLHTFFQTPSLYSLALSSDHAIASLFSDDNPHACSI
Sbjct: 121 PPSSNK-HHHHHHLNLSNSNNYSSQLHTFFQTPSLYSLALSSDHAIASLFSDDNPHACSI 180

[Close]

BLAST of CSPI03G47650 vs. NCBI nr
Match: XP_008465870.1 (PREDICTED: heavy metal-associated isoprenylated plant protein 20 isoform X2 [Cucumis melo])

HSP 1 Score: 303.5 bits (776), Expect = 1.1e-78
Identity = 163/179 (91.06%), Postives = 167/179 (93.30%), Query Frame = 0


Query: 3   MVEVRVPNLDCEGCASKLKKALFKLKGVEEVEVEIEMQKITVRGYGLEERKVVKAIKRAG 62
           MVEVRVPNLDCEGCASKL+KALFKLKGVEEVEVEIEMQKITVRGYGLEERKVVKAIKRAG
Sbjct: 1   MVEVRVPNLDCEGCASKLRKALFKLKGVEEVEVEIEMQKITVRGYGLEERKVVKAIKRAG 60

Query: 63  KAAEGWPFPGYSSHYTSFYKYPSYIANHYYDTYGGHN-----SNSNSNSNYSTTTT---- 122
           KAAEGWPFPGYSSHYTSFYKYPS IANHYYDTYGGHN     SNSNSNSNY+T+TT    
Sbjct: 61  KAAEGWPFPGYSSHYTSFYKYPSSIANHYYDTYGGHNSYSYYSNSNSNSNYTTSTTTTPP 120

Query: 123 SSNKHHHHHHHLNISNSNNCSSQLHTFFQTPSLYSLALSSDHAIASLFSDDNPHACSIM 173
           SSNK HHHHHHLN+SNSNN SSQLHTFFQTPSLYSLALSSDHAIASLFSDDNPHACSIM
Sbjct: 121 SSNK-HHHHHHLNLSNSNNYSSQLHTFFQTPSLYSLALSSDHAIASLFSDDNPHACSIM 178

[Close]

BLAST of CSPI03G47650 vs. NCBI nr
Match: XP_038888453.1 (heavy metal-associated isoprenylated plant protein 31 [Benincasa hispida])

HSP 1 Score: 249.6 bits (636), Expect = 2.0e-62
Identity = 133/172 (77.33%), Postives = 145/172 (84.30%), Query Frame = 0


Query: 1   MSMVEVRVPNLDCEGCASKLKKALFKLKGVEEVEVEIEMQKITVRGYGLEERKVVKAIKR 60
           MSMVEVRVPNLDCEGCASKL++ALFKLKGVEEVEVEIEMQKITVRGYGLEERKVVKAIKR
Sbjct: 1   MSMVEVRVPNLDCEGCASKLRRALFKLKGVEEVEVEIEMQKITVRGYGLEERKVVKAIKR 60

Query: 61  AGKAAEGWPFPGYSSHYTSFYKYPSYIANHYYDTYGGHNSNSNSNSNYSTTTTSSNKHHH 120
           AGKAAE WPFPGYSSHYTSFYKYPSYI NHYYDTY        S++NY+    ++NK HH
Sbjct: 61  AGKAAEAWPFPGYSSHYTSFYKYPSYIVNHYYDTY--------SSTNYNNRHHNNNKQHH 120

Query: 121 HHHHLNISNSNNCSSQLHTFFQTPSLYSLALSSDHAIASLFSDDNPHACSIM 173
                N +   + ++ LHTFFQTPSLYSLA+SSDH IASLFSDDNPHACSIM
Sbjct: 121 LISSTNTTAGGSPTNSLHTFFQTPSLYSLAVSSDHTIASLFSDDNPHACSIM 164

[Close]

BLAST of CSPI03G47650 vs. NCBI nr
Match: XP_022971858.1 (heavy metal-associated isoprenylated plant protein 31 isoform X1 [Cucurbita maxima])

HSP 1 Score: 236.9 bits (603), Expect = 1.3e-58
Identity = 128/172 (74.42%), Postives = 134/172 (77.91%), Query Frame = 0


Query: 1   MSMVEVRVPNLDCEGCASKLKKALFKLKGVEEVEVEIEMQKITVRGYGLEERKVVKAIKR 60
           MSM+EVRVPNLDCEGCASKL+KALFKLKGVEEVEVEIEMQKITVRGYGLEERKVVKAIKR
Sbjct: 14  MSMIEVRVPNLDCEGCASKLRKALFKLKGVEEVEVEIEMQKITVRGYGLEERKVVKAIKR 73

Query: 61  AGKAAEGWPFPGYSSHYTSFYKYPSYIANHYYDTYGGHNSNSNSNSNYSTTTTSSNKHHH 120
           AGKAAE WPFPGYSSHY SFYKYPSYIA HYYD              YS TT  +  +HH
Sbjct: 74  AGKAAEAWPFPGYSSHYASFYKYPSYIATHYYDA-------------YSATTNPTTTNHH 133

Query: 121 HHHHLNISNSNNCSSQLHTFFQTPSLYSLALSSDHAIASLFSDDNPHACSIM 173
                         + LHTFFQTPSLYS A+SSDHAIASLFSDDNPHACSIM
Sbjct: 134 -----------ASPTSLHTFFQTPSLYSRAVSSDHAIASLFSDDNPHACSIM 161

[Close]

BLAST of CSPI03G47650 vs. TAIR 10
Match: AT3G48970.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 169.1 bits (427), Expect = 3.1e-42
Identity = 94/173 (54.34%), Postives = 116/173 (67.05%), Query Frame = 0


Query: 1   MSM-VEVRVPNLDCEGCASKLKKALFKLKGVEEVEVEIEMQKITVRGYGLEERKVVKAIK 60
           MSM VE+RVPNLDCEGCASKL+K L KLKGVEEVEVE+E QK+T RGY LEE+KV+KA++
Sbjct: 1   MSMTVEIRVPNLDCEGCASKLRKTLLKLKGVEEVEVEMETQKVTARGYRLEEKKVLKAVR 60

Query: 61  RAGKAAEGWPFPGYSSHYTSFYKYPSYIANHYYDTYGGHNSNSNSNSNYSTTTTSSNKHH 120
           RAGKAAE WP+   +SH+ SFYKYPSY+ NHYY                      S+ H 
Sbjct: 61  RAGKAAELWPYRLGNSHFASFYKYPSYVTNHYY----------------------SDAHR 120

Query: 121 HHHHHLNISNSNNCSSQLHTFFQTPSLYSLALSSDHAIASLFSDDNPHACSIM 173
                       + +  +HTFF TP+ YS+A++ D   AS+FSDDNPHAC+IM
Sbjct: 121 -----------TDPTGGVHTFFHTPADYSVAVAGDEIAASMFSDDNPHACTIM 140

[Close]

BLAST of CSPI03G47650 vs. TAIR 10
Match: AT3G56891.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 80.5 bits (197), Expect = 1.5e-15
Identity = 60/172 (34.88%), Postives = 99/172 (57.56%), Query Frame = 0


Query: 1   MSMVEVRVPNLDCEGCASKLKKALFKLKGVEEVEVEIEMQKITVRGYGLEERKVVKAIKR 60
           +S+VE+ V ++DC+GC  K+++A+ KL GV+ VE++++ QK+TV GY ++  +V+K +KR
Sbjct: 15  LSIVELLV-DMDCKGCEKKVRRAISKLDGVDTVEIDVDRQKVTVTGY-VDREEVLKMVKR 74

Query: 61  AGKAAEGWPFPGYSSHYTSFYKYPSYIANHYYDTYGGHNSNSNSNSNYSTTTTSSNKHHH 120
            G+ AE WPFP Y+ +Y  +Y YPS                  S+     T + S K+  
Sbjct: 75  TGRTAEYWPFP-YNGYYGDYYTYPS-------------QHLEQSDQKIYQTISYSGKYDF 134

Query: 121 HHHHLNISNSNNCSSQLHTFFQTPSLYSLALSSDHAIASLFSDDNPHACSIM 173
           +    +  N+NN  S ++ ++ + S   +  + D     LFSDDN HAC+IM
Sbjct: 135 YDVD-DFQNTNN--STINGYYPSSS-QKVQPNIDENALHLFSDDNAHACTIM 166

[Close]

BLAST of CSPI03G47650 vs. TAIR 10
Match: AT2G18196.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 77.4 bits (189), Expect = 1.2e-14
Identity = 52/160 (32.50%), Postives = 86/160 (53.75%), Query Frame = 0


Query: 13  CEGCASKLKKALFKLKGVEEVEVEIEMQKITVRGYGLEERKVVKAIKRAGKAAEGWPFPG 72
           C GC   +K A++KL+GV+ VEV +EM+++TV GY +E +KV+KA++RAGK AE WP+P 
Sbjct: 56  CSGCERVVKHAIYKLRGVDSVEVNLEMERVTVVGY-VERKKVLKAVRRAGKRAEFWPYPD 115

Query: 73  YSSHYTSFYKYPSYIANHYYDTYGGHNSNSNSNSNYSTTTTSSNKHHHHHHHLNISNSNN 132
              ++TS        ++HY+                 TT      ++++ H  N+S+ + 
Sbjct: 116 MPRYFTS--------SDHYFK---------------DTTREFRESYNYYRHGYNLSDRH- 175

Query: 133 CSSQLHTFFQTPSLYSLALSSDHAIASLFSDDNPHACSIM 173
               +H          +    D  +++ F+DDN HACS+M
Sbjct: 176 --GNIH----------VTNRGDDKMSNFFNDDNVHACSLM 178

[Close]

BLAST of CSPI03G47650 vs. TAIR 10
Match: AT1G06330.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 70.1 bits (170), Expect = 2.0e-12
Identity = 57/172 (33.14%), Postives = 96/172 (55.81%), Query Frame = 0


Query: 1   MSMVEVRVPNLDCEGCASKLKKALFKLKGVEEVEVEIEMQKITVRGYGLEERKVVKAIKR 60
           +  +E+RV ++DC GC S++K AL K++GV+ VE+++  QK+TV GY  +++KV+K +++
Sbjct: 10  LQTIEMRV-HMDCVGCESRVKNALQKMRGVDAVEIDMVQQKVTVTGYA-DQKKVLKKVRK 69

Query: 61  AGKAAEGWPFPGYSSHYTSFYKYPSYIANHYYDTYGGHNSNSNSNSNYSTTTTSSNKHHH 120
            G+ AE W  P    H        S    ++Y+  G      N   N++    +S+ +++
Sbjct: 70  TGRRAELWQLPYNPDHMGG----SSSNGGYFYNPQG-----CNGPINHAAPVPTSSYNYY 129

Query: 121 HHHHLNISNSNNCSSQLHTFFQTPSLYSLALSSDHAIASLFSDDNPHACSIM 173
            H +    +SN+ SS  H      S++S      H   S FSD+NP+ACSIM
Sbjct: 130 KHGY----DSNDYSSYRHHPVHA-SIFS------HQTGSKFSDENPNACSIM 159

[Close]

BLAST of CSPI03G47650 vs. TAIR 10
Match: AT4G39700.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 60.1 bits (144), Expect = 2.1e-09
Identity = 56/169 (33.14%), Postives = 76/169 (44.97%), Query Frame = 0


Query: 4   VEVRVPNLDCEGCASKLKKALFKLKGVEEVEVEIEMQKITVRGYGLEERKVVKAIKRAGK 63
           VE++V  +DC+GC  K+K +L  LKGV+ VE+  + QK+TV GY  +  KV+K  K  GK
Sbjct: 34  VELKV-RMDCDGCVLKIKNSLSSLKGVKTVEINKKQQKVTVSGYA-DASKVLKKAKATGK 93

Query: 64  AAEGWPFPGYSSHYTSFYKYPSYIANHYYDTYGGHNSNSNSNSNYSTTTTSSNKHHHHHH 123
            AE WP+  Y+           YIA   YD           + N +T T           
Sbjct: 94  KAEIWPYVPYN------LVAQPYIA-QAYDKKAPPGYVRKVDPNVTTGT----------- 153

Query: 124 HLNISNSNNCSSQLHTFFQTPSLYSLALSSDHAIASLFSDDNPHACSIM 173
                        +  ++  PS             SLFSDDNP+ACSIM
Sbjct: 154 -------------MAVYYDDPS-----------YTSLFSDDNPNACSIM 158

The following BLAST results are available for this feature:

BLAST of CSPI03G47650 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q84K70	4.4e-41	54.34	Heavy metal-associated isoprenylated plant protein 31 OS=Arabidopsis thaliana OX...	[more]
B3H6D0	2.1e-14	34.88	Heavy metal-associated isoprenylated plant protein 45 OS=Arabidopsis thaliana OX...	[more]
F4IQG4	1.8e-13	32.50	Heavy metal-associated isoprenylated plant protein 30 OS=Arabidopsis thaliana OX...	[more]
F4IC29	2.8e-11	33.14	Heavy metal-associated isoprenylated plant protein 28 OS=Arabidopsis thaliana OX...	[more]
O65657	2.9e-08	33.14	Heavy metal-associated isoprenylated plant protein 23 OS=Arabidopsis thaliana OX...	[more]

BLAST of CSPI03G47650 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LHJ7	6.6e-88	99.42	HMA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G914050 PE=4 SV...	[more]
A0A1S3CQ83	5.0e-80	91.16	heavy metal-associated isoprenylated plant protein 20 isoform X1 OS=Cucumis melo...	[more]
A0A1S3CRB2	5.6e-79	91.06	heavy metal-associated isoprenylated plant protein 20 isoform X2 OS=Cucumis melo...	[more]
A0A6J1I865	6.4e-59	74.42	heavy metal-associated isoprenylated plant protein 31 isoform X1 OS=Cucurbita ma...	[more]
A0A6J1F0F2	1.1e-58	74.42	heavy metal-associated isoprenylated plant protein 31 isoform X1 OS=Cucurbita mo...	[more]

BLAST of CSPI03G47650 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004148352.2	1.4e-87	99.42	heavy metal-associated isoprenylated plant protein 31 [Cucumis sativus] >KGN6047...	[more]
XP_008465869.1	1.0e-79	91.16	PREDICTED: heavy metal-associated isoprenylated plant protein 20 isoform X1 [Cuc...	[more]
XP_008465870.1	1.1e-78	91.06	PREDICTED: heavy metal-associated isoprenylated plant protein 20 isoform X2 [Cuc...	[more]
XP_038888453.1	2.0e-62	77.33	heavy metal-associated isoprenylated plant protein 31 [Benincasa hispida]	[more]
XP_022971858.1	1.3e-58	74.42	heavy metal-associated isoprenylated plant protein 31 isoform X1 [Cucurbita maxi...	[more]

BLAST of CSPI03G47650 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G48970.1	3.1e-42	54.34	Heavy metal transport/detoxification superfamily protein	[more]
AT3G56891.1	1.5e-15	34.88	Heavy metal transport/detoxification superfamily protein	[more]
AT2G18196.1	1.2e-14	32.50	Heavy metal transport/detoxification superfamily protein	[more]
AT1G06330.1	2.0e-12	33.14	Heavy metal transport/detoxification superfamily protein	[more]
AT4G39700.1	2.1e-09	33.14	Heavy metal transport/detoxification superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006121	Heavy metal-associated domain, HMA	PFAM	PF00403	HMA	coord: 6..63 e-value: 3.8E-13 score: 49.6
IPR006121	Heavy metal-associated domain, HMA	PROSITE	PS50846	HMA_2	coord: 3..67 score: 14.555202
None	No IPR available	GENE3D	3.30.70.100		coord: 2..67 e-value: 1.3E-14 score: 56.1
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 103..127
None	No IPR available	PANTHER	PTHR22814	COPPER TRANSPORT PROTEIN ATOX1-RELATED	coord: 1..172
None	No IPR available	PANTHER	PTHR22814:SF319	HEAVY METAL-ASSOCIATED ISOPRENYLATED PLANT PROTEIN 31	coord: 1..172
IPR036163	Heavy metal-associated domain superfamily	SUPERFAMILY	55008	HMA, heavy metal-associated domain	coord: 2..68

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G47650.1	CSPI03G47650.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0046872	metal ion binding