CSPI03G47620 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI03G47620
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Serine proteinase inhibitor
Location	Chr3: 40595027 .. 40595891 (+)
RNA-Seq Expression	CSPI03G47620
Synteny	CSPI03G47620

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GAAAGGGGGAAAAGAAAAAAGAAAAGAATACCTCACGTTTTGTTGTATTCAGCAGTGCTAAATCTCATCTCAATTTTCTGAGCAAACGACTACAAGGGTAAAACAGGAAAAGAAACTGCATGAACTATATACTCCAATATGAATTTAATTGATTTTATTATGGTTTTTTGTATTTAATAATATTGTATACATATGTTATAGGTATGTATATATATTATTTTTATTGGATGGAATAAGAATAGATGGATTTATATGTTTATTATATAGGGAGGAAGATGAGAAAATTGGGTATAAATACAGGTATCCAATTCACTACAAAACACATCCCTAAGCTAAACTTTGTAAAGCATAACAAATAGAAAGACAACAAAACTCCAACATCATGTCCTCCGCATCCCATCATCAACGTAAGTATATCTTTTGGTCTAATATTTTTGTTGAGTTCATCTCTCTTTTCAATCTTCCAAGTTATATATAATTTTTAATATTTGCTAATTAACAAATATATATATATATTTCTTGTCTATGTGTTTCAAACCAGTAAAATCATGGCCAGCACTAAACTTCATGGATGCACGAACTGTGGCAAATATCATCAAGAAAGAGAATCCTGACTTTCAAATATTCATATTGTTGGCTGGAACTCCTGTAACAAAGGATCTTAAACATGGTCGAGTTCGACTCTTTACGAATATGGAGGAAATAGTGGTTATTGTTCCTCAAGAGGGCTGAAATGTAAACCCTACCTCTTCAAATAATAAATAATATATAAAAGTAATAAACTACTTCTTCTACTACTCCACATATATAAATTCCACATATATAAATAATTCAAATGATTGCAAAAAGTAAAAAATGAAATGGA

mRNA sequence

GAAAGGGGGAAAAGAAAAAAGAAAAGAATACCTCACGTTTTGTTGTATTCAGCAGTGCTAAATCTCATCTCAATTTTCTGAGCAAACGACTACAAGGGGAGGAAGATGAGAAAATTGGGTATAAATACAGGTATCCAATTCACTACAAAACACATCCCTAAGCTAAACTTTGTAAAGCATAACAAATAGAAAGACAACAAAACTCCAACATCATGTCCTCCGCATCCCATCATCAACTAAAATCATGGCCAGCACTAAACTTCATGGATGCACGAACTGTGGCAAATATCATCAAGAAAGAGAATCCTGACTTTCAAATATTCATATTGTTGGCTGGAACTCCTGTAACAAAGGATCTTAAACATGGTCGAGTTCGACTCTTTACGAATATGGAGGAAATAGTGGTTATTGTTCCTCAAGAGGGCTGAAATGTAAACCCTACCTCTTCAAATAATAAATAATATATAAAAGTAATAAACTACTTCTTCTACTACTCCACATATATAAATTCCACATATATAAATAATTCAAATGATTGCAAAAAGTAAAAAATGAAATGGA

Coding sequence (CDS)

ATGTCCTCCGCATCCCATCATCAACTAAAATCATGGCCAGCACTAAACTTCATGGATGCACGAACTGTGGCAAATATCATCAAGAAAGAGAATCCTGACTTTCAAATATTCATATTGTTGGCTGGAACTCCTGTAACAAAGGATCTTAAACATGGTCGAGTTCGACTCTTTACGAATATGGAGGAAATAGTGGTTATTGTTCCTCAAGAGGGCTGA

Protein sequence

MSSASHHQLKSWPALNFMDARTVANIIKKENPDFQIFILLAGTPVTKDLKHGRVRLFTNMEEIVVIVPQEG*

Homology

[Close]

BLAST of CSPI03G47620 vs. ExPASy Swiss-Prot
Match: Q03199 (Proteinase inhibitor I-B OS=Nicotiana tabacum OX=4097 GN=TIMPA PE=2 SV=1)

HSP 1 Score: 55.5 bits (132), Expect = 3.0e-07
Identity = 29/61 (47.54%), Postives = 37/61 (60.66%), Query Frame = 0


Query: 12  WPALNFMDARTVANIIKKENPDF-QIFILLAGTPVTKDLKHGRVRLFTNMEEIVVIVPQE 71
           WP L    A+    II+KENP    +  +L GTPVT+DL+  RVRLF N+ + VV  PQ 
Sbjct: 47  WPELLGTPAKFAMQIIQKENPKLTNVQTVLNGTPVTEDLRCNRVRLFVNVLDFVVQTPQV 106

[Close]

BLAST of CSPI03G47620 vs. ExPASy Swiss-Prot
Match: P20076 (Ethylene-responsive proteinase inhibitor 1 OS=Solanum lycopersicum OX=4081 PE=3 SV=1)

HSP 1 Score: 52.4 bits (124), Expect = 2.5e-06
Identity = 28/63 (44.44%), Postives = 38/63 (60.32%), Query Frame = 0


Query: 10  KSWPALNFMDARTVANIIKKENPDF-QIFILLAGTPVTKDLKHGRVRLFTNMEEIVVIVP 69
           +SWP L    A+    II+KENP    +  LL G+  T+DL+  RVRLF N+ +IVV  P
Sbjct: 57  ESWPELLGTPAKFAKQIIQKENPKLTNVETLLNGSAFTEDLRCNRVRLFVNLLDIVVQTP 116

Query: 70  QEG 72
           + G
Sbjct: 117 KVG 119

[Close]

BLAST of CSPI03G47620 vs. ExPASy Swiss-Prot
Match: Q03198 (Proteinase inhibitor I-A OS=Nicotiana tabacum OX=4097 GN=TIMPB PE=2 SV=1)

HSP 1 Score: 52.4 bits (124), Expect = 2.5e-06
Identity = 27/61 (44.26%), Postives = 36/61 (59.02%), Query Frame = 0


Query: 12  WPALNFMDARTVANIIKKENPDF-QIFILLAGTPVTKDLKHGRVRLFTNMEEIVVIVPQE 71
           WP L    A+    II+KENP    +  +L G PVT+DL+  RVRLF N+ + +V  PQ 
Sbjct: 47  WPELLGTPAKFAMQIIQKENPKLTNVQTILNGGPVTEDLRCNRVRLFVNVLDFIVQTPQI 106

[Close]

BLAST of CSPI03G47620 vs. ExPASy Swiss-Prot
Match: P19873 (Inhibitor of trypsin and hageman factor OS=Cucurbita maxima OX=3661 PE=1 SV=1)

HSP 1 Score: 52.4 bits (124), Expect = 2.5e-06
Identity = 25/61 (40.98%), Postives = 36/61 (59.02%), Query Frame = 0


Query: 11 SWPALNFMDARTVANIIKKENPDFQIFILLAGTPVTKDLKHGRVRLFTNMEEIVVIVPQE 70
          SWP L  +       II+++NP+ +  IL  GTPVTKD +  RVR++ N   +VV  P+ 
Sbjct: 8  SWPHLVGVGGSVAKAIIERQNPNVKAVILEEGTPVTKDFRCNRVRIWVNKRGLVVSPPRI 67

Query: 71 G 72
          G
Sbjct: 68 G 68

[Close]

BLAST of CSPI03G47620 vs. ExPASy Swiss-Prot
Match: Q02214 (Trypsin inhibitor 1 OS=Nicotiana sylvestris OX=4096 PE=2 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 3.3e-06
Identity = 28/71 (39.44%), Postives = 43/71 (60.56%), Query Frame = 0


Query: 2  SSASHHQLKSWPALNFMDARTVANIIKKENPDF-QIFILLAGTPVTKDLKHGRVRLFTNM 61
          SS   ++ ++WP L  + A+    II+KEN     +  +L G+PVTKD +  RVRLF N+
Sbjct: 24 SSCPGNKKETWPELIGVPAKFAREIIQKENSKLTNVPSVLNGSPVTKDFRCERVRLFVNV 83

Query: 62 EEIVVIVPQEG 72
           + VV +P+ G
Sbjct: 84 LDFVVQIPRVG 94

[Close]

BLAST of CSPI03G47620 vs. ExPASy TrEMBL
Match: A0A0A0LIF6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G914570 PE=3 SV=1)

HSP 1 Score: 146.0 bits (367), Expect = 6.2e-32
Identity = 70/71 (98.59%), Postives = 71/71 (100.00%), Query Frame = 0


Query: 1  MSSASHHQLKSWPALNFMDARTVANIIKKENPDFQIFILLAGTPVTKDLKHGRVRLFTNM 60
          MSSASHHQLKSWPALNFMDARTVANIIKKENPDFQIFILLAG+PVTKDLKHGRVRLFTNM
Sbjct: 1  MSSASHHQLKSWPALNFMDARTVANIIKKENPDFQIFILLAGSPVTKDLKHGRVRLFTNM 60

Query: 61 EEIVVIVPQEG 72
          EEIVVIVPQEG
Sbjct: 61 EEIVVIVPQEG 71

[Close]

BLAST of CSPI03G47620 vs. ExPASy TrEMBL
Match: A0A0A0LFD4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G914580 PE=3 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 8.4e-13
Identity = 40/63 (63.49%), Postives = 47/63 (74.60%), Query Frame = 0


Query: 9  LKSWPALNFMDARTVANIIKKENPDFQIFILLAGTPVTKDLKHGRVRLFTNMEEIVVIVP 68
          LKSWPAL F+DA+TVAN IK ENP+F+I  +  GTPVTKD+  GRVRL  +    VV VP
Sbjct: 7  LKSWPALKFLDAQTVANAIKTENPNFEIVTVPEGTPVTKDIIPGRVRLVVDANGKVVNVP 66

Query: 69 QEG 72
          QEG
Sbjct: 67 QEG 69

[Close]

BLAST of CSPI03G47620 vs. ExPASy TrEMBL
Match: A0A0A0KPI0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G027950 PE=3 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 3.1e-07
Identity = 28/61 (45.90%), Postives = 42/61 (68.85%), Query Frame = 0


Query: 11 SWPALNFMDARTVANIIKKENPDFQIFILLAGTPVTKDLKHGRVRLFTNMEEIVVIVPQE 70
          SWP L F+ +    + I+++ PD +  +LLAG+PVT+DL+  RVR+F NM + VV VP+ 
Sbjct: 15 SWPELVFVKSSVAVHWIERDRPDVKPVVLLAGSPVTEDLRPNRVRIFVNMNDRVVEVPRT 74

Query: 71 G 72
          G
Sbjct: 75 G 75

[Close]

BLAST of CSPI03G47620 vs. ExPASy TrEMBL
Match: A0A397ZK38 (Uncharacterized protein OS=Brassica campestris OX=3711 GN=BRARA_D01163 PE=3 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 4.0e-07
Identity = 29/63 (46.03%), Postives = 40/63 (63.49%), Query Frame = 0


Query: 9  LKSWPALNFMDARTVANIIKKENPDFQIFILLAGTPVTKDLKHGRVRLFTNMEEIVVIVP 68
          LK+WP L        A++IK+ENP F +F+LL G+ VT DL+  RVR++ +   IVV VP
Sbjct: 3  LKTWPELVGTHGDDAASVIKRENPSFNVFVLLIGSIVTADLRENRVRVWVDTNRIVVRVP 62

Query: 69 QEG 72
            G
Sbjct: 63 TTG 65

[Close]

BLAST of CSPI03G47620 vs. ExPASy TrEMBL
Match: A0A6J1CY53 (inhibitor of trypsin and hageman factor-like OS=Momordica charantia OX=3673 GN=LOC111015335 PE=3 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 1.3e-05
Identity = 27/62 (43.55%), Postives = 40/62 (64.52%), Query Frame = 0


Query: 10 KSWPALNFMDARTVANIIKKENPDFQIFILLAGTPVTKDLKHGRVRLFTNMEEIVVIVPQ 69
          + WP L  +     A IIK+EN   +  +LLAG+PVT D +  RVR+F N+E++VV +P+
Sbjct: 18 QEWPELVGVKVSVAALIIKRENAKLEPTVLLAGSPVTLDYRPYRVRIFKNIEDVVVGIPR 77

Query: 70 EG 72
           G
Sbjct: 78 TG 79

[Close]

BLAST of CSPI03G47620 vs. NCBI nr
Match: KGN60477.1 (hypothetical protein Csa_001998 [Cucumis sativus])

HSP 1 Score: 146.0 bits (367), Expect = 1.3e-31
Identity = 70/71 (98.59%), Postives = 71/71 (100.00%), Query Frame = 0


Query: 1  MSSASHHQLKSWPALNFMDARTVANIIKKENPDFQIFILLAGTPVTKDLKHGRVRLFTNM 60
          MSSASHHQLKSWPALNFMDARTVANIIKKENPDFQIFILLAG+PVTKDLKHGRVRLFTNM
Sbjct: 1  MSSASHHQLKSWPALNFMDARTVANIIKKENPDFQIFILLAGSPVTKDLKHGRVRLFTNM 60

Query: 61 EEIVVIVPQEG 72
          EEIVVIVPQEG
Sbjct: 61 EEIVVIVPQEG 71

[Close]

BLAST of CSPI03G47620 vs. NCBI nr
Match: KAG6571807.1 (hypothetical protein SDJN03_28535, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 97.1 bits (240), Expect = 6.8e-17
Identity = 45/63 (71.43%), Postives = 53/63 (84.13%), Query Frame = 0


Query: 9  LKSWPALNFMDARTVANIIKKENPDFQIFILLAGTPVTKDLKHGRVRLFTNMEEIVVIVP 68
          LKSWP LN M A TV NIIK+++P+FQI ILLAG+PVTKDLKHGRVRLF N+  IVV +P
Sbjct: 5  LKSWPELNLMQASTVENIIKRQHPEFQIVILLAGSPVTKDLKHGRVRLFVNILGIVVEIP 64

Query: 69 QEG 72
          +EG
Sbjct: 65 REG 67

[Close]

BLAST of CSPI03G47620 vs. NCBI nr
Match: KGN60478.1 (hypothetical protein Csa_001396 [Cucumis sativus])

HSP 1 Score: 82.4 bits (202), Expect = 1.7e-12
Identity = 40/63 (63.49%), Postives = 47/63 (74.60%), Query Frame = 0


Query: 9  LKSWPALNFMDARTVANIIKKENPDFQIFILLAGTPVTKDLKHGRVRLFTNMEEIVVIVP 68
          LKSWPAL F+DA+TVAN IK ENP+F+I  +  GTPVTKD+  GRVRL  +    VV VP
Sbjct: 7  LKSWPALKFLDAQTVANAIKTENPNFEIVTVPEGTPVTKDIIPGRVRLVVDANGKVVNVP 66

Query: 69 QEG 72
          QEG
Sbjct: 67 QEG 69

[Close]

BLAST of CSPI03G47620 vs. NCBI nr
Match: XP_011654473.1 (inhibitor of trypsin and hageman factor [Cucumis sativus] >KGN49621.1 hypothetical protein Csa_018430 [Cucumis sativus])

HSP 1 Score: 63.9 bits (154), Expect = 6.4e-07
Identity = 28/61 (45.90%), Postives = 42/61 (68.85%), Query Frame = 0


Query: 11 SWPALNFMDARTVANIIKKENPDFQIFILLAGTPVTKDLKHGRVRLFTNMEEIVVIVPQE 70
          SWP L F+ +    + I+++ PD +  +LLAG+PVT+DL+  RVR+F NM + VV VP+ 
Sbjct: 15 SWPELVFVKSSVAVHWIERDRPDVKPVVLLAGSPVTEDLRPNRVRIFVNMNDRVVEVPRT 74

Query: 71 G 72
          G
Sbjct: 75 G 75

[Close]

BLAST of CSPI03G47620 vs. NCBI nr
Match: RID65997.1 (hypothetical protein BRARA_D01163 [Brassica rapa])

HSP 1 Score: 63.5 bits (153), Expect = 8.3e-07
Identity = 29/63 (46.03%), Postives = 40/63 (63.49%), Query Frame = 0


Query: 9  LKSWPALNFMDARTVANIIKKENPDFQIFILLAGTPVTKDLKHGRVRLFTNMEEIVVIVP 68
          LK+WP L        A++IK+ENP F +F+LL G+ VT DL+  RVR++ +   IVV VP
Sbjct: 3  LKTWPELVGTHGDDAASVIKRENPSFNVFVLLIGSIVTADLRENRVRVWVDTNRIVVRVP 62

Query: 69 QEG 72
            G
Sbjct: 63 TTG 65

[Close]

BLAST of CSPI03G47620 vs. TAIR 10
Match: AT2G38870.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 52.4 bits (124), Expect = 1.8e-07
Identity = 24/61 (39.34%), Postives = 35/61 (57.38%), Query Frame = 0


Query: 11 SWPALNFMDARTVANIIKKENPDFQIFILLAGTPVTKDLKHGRVRLFTNMEEIVVIVPQE 70
          SWP L   +    A +I++ENP     ++L G+PVT D +  RVR+F +   IVV  P+ 
Sbjct: 10 SWPELTGTNGDYAAVVIERENPTVNAAVILDGSPVTADFRCDRVRVFVDGNRIVVKTPKS 69

Query: 71 G 72
          G
Sbjct: 70 G 70

The following BLAST results are available for this feature:

BLAST of CSPI03G47620 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q03199	3.0e-07	47.54	Proteinase inhibitor I-B OS=Nicotiana tabacum OX=4097 GN=TIMPA PE=2 SV=1	[more]
P20076	2.5e-06	44.44	Ethylene-responsive proteinase inhibitor 1 OS=Solanum lycopersicum OX=4081 PE=3 ...	[more]
Q03198	2.5e-06	44.26	Proteinase inhibitor I-A OS=Nicotiana tabacum OX=4097 GN=TIMPB PE=2 SV=1	[more]
P19873	2.5e-06	40.98	Inhibitor of trypsin and hageman factor OS=Cucurbita maxima OX=3661 PE=1 SV=1	[more]
Q02214	3.3e-06	39.44	Trypsin inhibitor 1 OS=Nicotiana sylvestris OX=4096 PE=2 SV=1	[more]

BLAST of CSPI03G47620 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LIF6	6.2e-32	98.59	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G914570 PE=3 SV=1	[more]
A0A0A0LFD4	8.4e-13	63.49	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G914580 PE=3 SV=1	[more]
A0A0A0KPI0	3.1e-07	45.90	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G027950 PE=3 SV=1	[more]
A0A397ZK38	4.0e-07	46.03	Uncharacterized protein OS=Brassica campestris OX=3711 GN=BRARA_D01163 PE=3 SV=1	[more]
A0A6J1CY53	1.3e-05	43.55	inhibitor of trypsin and hageman factor-like OS=Momordica charantia OX=3673 GN=L...	[more]

BLAST of CSPI03G47620 vs. NCBI nr


Match Name	E-value	Identity	Description
KGN60477.1	1.3e-31	98.59	hypothetical protein Csa_001998 [Cucumis sativus]	[more]
KAG6571807.1	6.8e-17	71.43	hypothetical protein SDJN03_28535, partial [Cucurbita argyrosperma subsp. sorori...	[more]
KGN60478.1	1.7e-12	63.49	hypothetical protein Csa_001396 [Cucumis sativus]	[more]
XP_011654473.1	6.4e-07	45.90	inhibitor of trypsin and hageman factor [Cucumis sativus] >KGN49621.1 hypothetic...	[more]
RID65997.1	8.3e-07	46.03	hypothetical protein BRARA_D01163 [Brassica rapa]	[more]

BLAST of CSPI03G47620 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G38870.1	1.8e-07	39.34	Serine protease inhibitor, potato inhibitor I-type family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PRINTS	PR00292	POTATOINHBTR	coord: 11..24 score: 26.4 coord: 36..47 score: 44.57 coord: 48..58 score: 47.04
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PFAM	PF00280	potato_inhibit	coord: 11..69 e-value: 1.6E-13 score: 51.1
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PANTHER	PTHR33091	PROTEIN, PUTATIVE, EXPRESSED-RELATED	coord: 5..71
None	No IPR available	GENE3D	3.30.10.10	Trypsin Inhibitor V, subunit A	coord: 2..71 e-value: 9.2E-16 score: 59.8
None	No IPR available	PANTHER	PTHR33091:SF69	SUBFAMILY NOT NAMED	coord: 5..71
IPR036354	Proteinase inhibitor I13, potato inhibitor I superfamily	SUPERFAMILY	54654	CI-2 family of serine protease inhibitors	coord: 8..69

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G47620.1	CSPI03G47620.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
biological_process	GO:0009611	response to wounding
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity