CSPI03G47210 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI03G47210
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Descriptioncation/H(+) antiporter 1-like
LocationChr3: 40244251 .. 40246998 (+)
RNA-Seq ExpressionCSPI03G47210
SyntenyCSPI03G47210
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACGCAACACATAGAATGGTTTGCCAAGACGACTTGTTCAACCCTTTGTCGTCAATGGGTGTTCAGGTTTCCTTCATTCTTGTCCTTTCTCACTTCTTCCATCTCGTCCTCAAGGCCTTCGGCCAACCCGGTCCCATTGCCCAGATTCTGGTATTCTCATTACACATTTCGAAGTTTGTTTTGAGTGGTTGTGAATCATTATGATGTGTTTTTTTAAAATGATGGATTTTGCATCTGCATGCAGGCAGGCATGGTGTTGGGTCCTACTGGGCTATCAAACATCAAAGCTATACGAGATGTATTCTTTCAGGCTTCAGCTGCTGACTACTATGAAATCTTTGGCTTTTTAAGTCGAATCATTTTCATGTTTCTTATTGGCTTGGAGACCGATTTCCCCTACATTCTCCGCAACCTCCGCGTCGCTGGCATTGTTGCATGCGGTGGTGCTGCAGTCGGTAGCGTCTTCGGCATTGCCGTCTCCTTCTTCCTCTACCAACAATTCGAAGAAAAAAGCAGTAGGTTCGGTTTTTTCTTCATAGTCATGCTGATTCTAGCGTACACCGCTTCCCCGATTGTCATCCGTCTCGCGGCCGAGTTGAAGTTCGCGACTTCTGACGTGGGAAAACTCGCTATTTCATCCGCCCTCATCAATGAAATGGCATGTTTGGCGGTTTTCAATGCAATTTTAGCATTGAGATCATTCCAAGAGTTTGGAAAAGGAATATTTTGTGCTGTATTCATTGCTGGTGTTGTGATACTGAACAAATACTTAGCGAGTTGGTTCAACAAAAGAAACCGTAACCAAAAGTACCTTAAGAACATGGAAGTGTTTTTTTTGTTGTCTCTTGTGATTGCAGCATCGGTAATAATTGAGCTTCAAGCATTTAACAGCATTGTTAGCAGCTTTATTTTTGGGGTAATGTTTCCAAAAGAAGGCAAATCAGCAAGAACTTTAATGCACAAATTGACTTATTCAGTGCACAATTTTGTTTTACCAATCTATTTTGGTTACGTTGGATTCCAATTTGATGGGAACAATCTTTGGAAAATGAGCAATGTGATTATTGTGGGAATTATGGTATTGTTGAGTATTGGGAGCAAAATGAGTGGAACTTTAGCTGCATGCAACTACTTGAATATTCCTTTGAATGAAGGAGTTTTTCTTGGTTTTGTTCTTAACTTGAAGGGACATGCTGATCTCTTGCTTATTGGTGGAGCCTCCAAGTCTATCTTGGTAAGTAATCATTATGTATTTTAATTTTTTTTGTTGGATAACTTAACGTTAATTTAAACACATCCTTCCATCTTAAATTGCACTTAGTATTAATGTATAAAGTTAGGGATGATGATAACGTTTATAAAACTAACAATGGTCATGGTAGAGTTGTGTACCCTTTGGTTGCAGACATGGAGCAATCCTCGAGCTTACAACCTCCTCTTAATAAGCATAGTAATCAACACCATAATCTCAGGTCCTATCGTCGCCTTACTGATGCGACGTGAACACAAGCTATTTTCTCACGCCCACACCTCATTAGAGTACACGGACCCGACCCACGAGCTTCGTGCTCTAGCATGTGCCTATGGTCCTCGCCACCTAGCTGGCATATTCCCACTCCTCTCTTCCCTTAGCGGTGGTCACACTTCCCAGCTCTCCCCTTTCTTGCTCCACCTCATTGAACTCCTCCACAAGCGTCGTACTAATGTCTCGTACCACGAGCTTGAACAAGATGAATTGAGCGACGACGAAGGTTACGGAGGCAACGACGTGCTTGAAATCCATTGTGCCATCGATGCATTCATATCCGATACAAAAATATTCATGTCACTCTCAAAAGCTATCTCTGCTTTTCCTACCTTGTATGAGGATGTTTGTAATGCAGCTGAGGATTTGAGAGTTTCCATTGTGATTCTTCCATTTCATAAGCATCAAAGGATAGATGGGAAAATGGAGAGTGGGAAGGAAGGCATTAGAACAACCAACCAGAAGATTTTGCGCCATGCACCATGCTCAGTGGGAATTCTCGTGGACCGGGTTCAAACCGGGTTCTTGTCGTTCTCGCATTTGTTGGTATCCGATCATGTGCAGCATGTGGCGACGCTGTTTTTTGGTGGCCCAGATGATAGGGAGGCGTTGGCATGGAGTCGGAGAATGATTAGTCACTCAAGGATTAATTTGACCGTCATAAGGTTTGTCCCGAAAGCAACGTCTGATGTCGAGGGGACGGCCACAACATCGTCGAGTGATGATGGTGTTTTAATGGCTTTACCTAGTTTGAGAACCACTTCTAGTGAGACTGATAATACCTTCCTTGCTGATTTCTATGACAGGTGTTTGTTTGCATCATTGTTTCTTTCCCTTATTTGTTTGTTTTTAGTTTTATGTTTGGTTTCTAGCTCCTAAACTTCATACTGCTATGGTTTTTCACATTTGTATATTCATGGAATTCATTTCATGGTAGGCATGTCTCAACGGGACAAGTAGGGTATGTAGAGAAGCAAGTGAAGAATGGGGAAGAGACAGTGGCGGAGCTAAGAGACATAGGAGACATGTACTCTCTATTTATTGTGGGGAAAGGAGGGAGAGGTCACTCCCCTCTAACCACAGGGATGAGTGACTGGGAAGAGTGCTCCGAGCTCGGTACCGTCGGAGACCTCTTGGCTTCTTCAGACTTCAATATTAGTGGTTCCGTTTTGATCATTCAACAACATAGACACCAAAAGAAGGATCTTATTGATGACTAA

mRNA sequence

ATGGACGCAACACATAGAATGGTTTGCCAAGACGACTTGTTCAACCCTTTGTCGTCAATGGGTGTTCAGGTTTCCTTCATTCTTGTCCTTTCTCACTTCTTCCATCTCGTCCTCAAGGCCTTCGGCCAACCCGGTCCCATTGCCCAGATTCTGGCAGGCATGGTGTTGGGTCCTACTGGGCTATCAAACATCAAAGCTATACGAGATGTATTCTTTCAGGCTTCAGCTGCTGACTACTATGAAATCTTTGGCTTTTTAAGTCGAATCATTTTCATGTTTCTTATTGGCTTGGAGACCGATTTCCCCTACATTCTCCGCAACCTCCGCGTCGCTGGCATTGTTGCATGCGGTGGTGCTGCAGTCGGTAGCGTCTTCGGCATTGCCGTCTCCTTCTTCCTCTACCAACAATTCGAAGAAAAAAGCAGTAGGTTCGGTTTTTTCTTCATAGTCATGCTGATTCTAGCGTACACCGCTTCCCCGATTGTCATCCGTCTCGCGGCCGAGTTGAAGTTCGCGACTTCTGACGTGGGAAAACTCGCTATTTCATCCGCCCTCATCAATGAAATGGCATGTTTGGCGGTTTTCAATGCAATTTTAGCATTGAGATCATTCCAAGAGTTTGGAAAAGGAATATTTTGTGCTGTATTCATTGCTGGTGTTGTGATACTGAACAAATACTTAGCGAGTTGGTTCAACAAAAGAAACCGTAACCAAAAGTACCTTAAGAACATGGAAGTGTTTTTTTTGTTGTCTCTTGTGATTGCAGCATCGGTAATAATTGAGCTTCAAGCATTTAACAGCATTGTTAGCAGCTTTATTTTTGGGGTAATGTTTCCAAAAGAAGGCAAATCAGCAAGAACTTTAATGCACAAATTGACTTATTCAGTGCACAATTTTGTTTTACCAATCTATTTTGGTTACGTTGGATTCCAATTTGATGGGAACAATCTTTGGAAAATGAGCAATGTGATTATTGTGGGAATTATGGTATTGTTGAGTATTGGGAGCAAAATGAGTGGAACTTTAGCTGCATGCAACTACTTGAATATTCCTTTGAATGAAGGAGTTTTTCTTGGTTTTGTTCTTAACTTGAAGGGACATGCTGATCTCTTGCTTATTGGTGGAGCCTCCAAGTCTATCTTGACATGGAGCAATCCTCGAGCTTACAACCTCCTCTTAATAAGCATAGTAATCAACACCATAATCTCAGGTCCTATCGTCGCCTTACTGATGCGACGTGAACACAAGCTATTTTCTCACGCCCACACCTCATTAGAGTACACGGACCCGACCCACGAGCTTCGTGCTCTAGCATGTGCCTATGGTCCTCGCCACCTAGCTGGCATATTCCCACTCCTCTCTTCCCTTAGCGGTGGTCACACTTCCCAGCTCTCCCCTTTCTTGCTCCACCTCATTGAACTCCTCCACAAGCGTCGTACTAATGTCTCGTACCACGAGCTTGAACAAGATGAATTGAGCGACGACGAAGGTTACGGAGGCAACGACGTGCTTGAAATCCATTGTGCCATCGATGCATTCATATCCGATACAAAAATATTCATGTCACTCTCAAAAGCTATCTCTGCTTTTCCTACCTTGTATGAGGATGTTTGTAATGCAGCTGAGGATTTGAGAGTTTCCATTGTGATTCTTCCATTTCATAAGCATCAAAGGATAGATGGGAAAATGGAGAGTGGGAAGGAAGGCATTAGAACAACCAACCAGAAGATTTTGCGCCATGCACCATGCTCAGTGGGAATTCTCGTGGACCGGGTTCAAACCGGGTTCTTGTCGTTCTCGCATTTGTTGGTATCCGATCATGTGCAGCATGTGGCGACGCTGTTTTTTGGTGGCCCAGATGATAGGGAGGCGTTGGCATGGAGTCGGAGAATGATTAGTCACTCAAGGATTAATTTGACCGTCATAAGGTTTGTCCCGAAAGCAACGTCTGATGTCGAGGGGACGGCCACAACATCGTCGAGTGATGATGGTGTTTTAATGGCTTTACCTAGTTTGAGAACCACTTCTAGTGAGACTGATAATACCTTCCTTGCTGATTTCTATGACAGGCATGTCTCAACGGGACAAGTAGGGTATGTAGAGAAGCAAGTGAAGAATGGGGAAGAGACAGTGGCGGAGCTAAGAGACATAGGAGACATGTACTCTCTATTTATTGTGGGGAAAGGAGGGAGAGGTCACTCCCCTCTAACCACAGGGATGAGTGACTGGGAAGAGTGCTCCGAGCTCGGTACCGTCGGAGACCTCTTGGCTTCTTCAGACTTCAATATTAGTGGTTCCGTTTTGATCATTCAACAACATAGACACCAAAAGAAGGATCTTATTGATGACTAA

Coding sequence (CDS)

ATGGACGCAACACATAGAATGGTTTGCCAAGACGACTTGTTCAACCCTTTGTCGTCAATGGGTGTTCAGGTTTCCTTCATTCTTGTCCTTTCTCACTTCTTCCATCTCGTCCTCAAGGCCTTCGGCCAACCCGGTCCCATTGCCCAGATTCTGGCAGGCATGGTGTTGGGTCCTACTGGGCTATCAAACATCAAAGCTATACGAGATGTATTCTTTCAGGCTTCAGCTGCTGACTACTATGAAATCTTTGGCTTTTTAAGTCGAATCATTTTCATGTTTCTTATTGGCTTGGAGACCGATTTCCCCTACATTCTCCGCAACCTCCGCGTCGCTGGCATTGTTGCATGCGGTGGTGCTGCAGTCGGTAGCGTCTTCGGCATTGCCGTCTCCTTCTTCCTCTACCAACAATTCGAAGAAAAAAGCAGTAGGTTCGGTTTTTTCTTCATAGTCATGCTGATTCTAGCGTACACCGCTTCCCCGATTGTCATCCGTCTCGCGGCCGAGTTGAAGTTCGCGACTTCTGACGTGGGAAAACTCGCTATTTCATCCGCCCTCATCAATGAAATGGCATGTTTGGCGGTTTTCAATGCAATTTTAGCATTGAGATCATTCCAAGAGTTTGGAAAAGGAATATTTTGTGCTGTATTCATTGCTGGTGTTGTGATACTGAACAAATACTTAGCGAGTTGGTTCAACAAAAGAAACCGTAACCAAAAGTACCTTAAGAACATGGAAGTGTTTTTTTTGTTGTCTCTTGTGATTGCAGCATCGGTAATAATTGAGCTTCAAGCATTTAACAGCATTGTTAGCAGCTTTATTTTTGGGGTAATGTTTCCAAAAGAAGGCAAATCAGCAAGAACTTTAATGCACAAATTGACTTATTCAGTGCACAATTTTGTTTTACCAATCTATTTTGGTTACGTTGGATTCCAATTTGATGGGAACAATCTTTGGAAAATGAGCAATGTGATTATTGTGGGAATTATGGTATTGTTGAGTATTGGGAGCAAAATGAGTGGAACTTTAGCTGCATGCAACTACTTGAATATTCCTTTGAATGAAGGAGTTTTTCTTGGTTTTGTTCTTAACTTGAAGGGACATGCTGATCTCTTGCTTATTGGTGGAGCCTCCAAGTCTATCTTGACATGGAGCAATCCTCGAGCTTACAACCTCCTCTTAATAAGCATAGTAATCAACACCATAATCTCAGGTCCTATCGTCGCCTTACTGATGCGACGTGAACACAAGCTATTTTCTCACGCCCACACCTCATTAGAGTACACGGACCCGACCCACGAGCTTCGTGCTCTAGCATGTGCCTATGGTCCTCGCCACCTAGCTGGCATATTCCCACTCCTCTCTTCCCTTAGCGGTGGTCACACTTCCCAGCTCTCCCCTTTCTTGCTCCACCTCATTGAACTCCTCCACAAGCGTCGTACTAATGTCTCGTACCACGAGCTTGAACAAGATGAATTGAGCGACGACGAAGGTTACGGAGGCAACGACGTGCTTGAAATCCATTGTGCCATCGATGCATTCATATCCGATACAAAAATATTCATGTCACTCTCAAAAGCTATCTCTGCTTTTCCTACCTTGTATGAGGATGTTTGTAATGCAGCTGAGGATTTGAGAGTTTCCATTGTGATTCTTCCATTTCATAAGCATCAAAGGATAGATGGGAAAATGGAGAGTGGGAAGGAAGGCATTAGAACAACCAACCAGAAGATTTTGCGCCATGCACCATGCTCAGTGGGAATTCTCGTGGACCGGGTTCAAACCGGGTTCTTGTCGTTCTCGCATTTGTTGGTATCCGATCATGTGCAGCATGTGGCGACGCTGTTTTTTGGTGGCCCAGATGATAGGGAGGCGTTGGCATGGAGTCGGAGAATGATTAGTCACTCAAGGATTAATTTGACCGTCATAAGGTTTGTCCCGAAAGCAACGTCTGATGTCGAGGGGACGGCCACAACATCGTCGAGTGATGATGGTGTTTTAATGGCTTTACCTAGTTTGAGAACCACTTCTAGTGAGACTGATAATACCTTCCTTGCTGATTTCTATGACAGGCATGTCTCAACGGGACAAGTAGGGTATGTAGAGAAGCAAGTGAAGAATGGGGAAGAGACAGTGGCGGAGCTAAGAGACATAGGAGACATGTACTCTCTATTTATTGTGGGGAAAGGAGGGAGAGGTCACTCCCCTCTAACCACAGGGATGAGTGACTGGGAAGAGTGCTCCGAGCTCGGTACCGTCGGAGACCTCTTGGCTTCTTCAGACTTCAATATTAGTGGTTCCGTTTTGATCATTCAACAACATAGACACCAAAAGAAGGATCTTATTGATGACTAA

Protein sequence

MDATHRMVCQDDLFNPLSSMGVQVSFILVLSHFFHLVLKAFGQPGPIAQILAGMVLGPTGLSNIKAIRDVFFQASAADYYEIFGFLSRIIFMFLIGLETDFPYILRNLRVAGIVACGGAAVGSVFGIAVSFFLYQQFEEKSSRFGFFFIVMLILAYTASPIVIRLAAELKFATSDVGKLAISSALINEMACLAVFNAILALRSFQEFGKGIFCAVFIAGVVILNKYLASWFNKRNRNQKYLKNMEVFFLLSLVIAASVIIELQAFNSIVSSFIFGVMFPKEGKSARTLMHKLTYSVHNFVLPIYFGYVGFQFDGNNLWKMSNVIIVGIMVLLSIGSKMSGTLAACNYLNIPLNEGVFLGFVLNLKGHADLLLIGGASKSILTWSNPRAYNLLLISIVINTIISGPIVALLMRREHKLFSHAHTSLEYTDPTHELRALACAYGPRHLAGIFPLLSSLSGGHTSQLSPFLLHLIELLHKRRTNVSYHELEQDELSDDEGYGGNDVLEIHCAIDAFISDTKIFMSLSKAISAFPTLYEDVCNAAEDLRVSIVILPFHKHQRIDGKMESGKEGIRTTNQKILRHAPCSVGILVDRVQTGFLSFSHLLVSDHVQHVATLFFGGPDDREALAWSRRMISHSRINLTVIRFVPKATSDVEGTATTSSSDDGVLMALPSLRTTSSETDNTFLADFYDRHVSTGQVGYVEKQVKNGEETVAELRDIGDMYSLFIVGKGGRGHSPLTTGMSDWEECSELGTVGDLLASSDFNISGSVLIIQQHRHQKKDLIDD*
Homology
BLAST of CSPI03G47210 vs. ExPASy Swiss-Prot
Match: Q9SA37 (Cation/H(+) antiporter 1 OS=Arabidopsis thaliana OX=3702 GN=CHX1 PE=2 SV=1)

HSP 1 Score: 629.4 bits (1622), Expect = 5.4e-179
Identity = 347/783 (44.32%), Postives = 506/783 (64.62%), Query Frame = 0

Query: 11  DDLFNPLSSMGVQVSFILVLSHFFHLVLKAFGQPGPIAQILAGMVLGPTGLSNIKAIRDV 70
           D LFNPL++M +Q++ ILV S FF+L LK  GQ GP+AQILAG+VL  + L+ I+ + + 
Sbjct: 13  DALFNPLNTMFIQMACILVFSQFFYLFLKPCGQAGPVAQILAGIVL--SLLTIIRKVHEF 72

Query: 71  FFQASAADYYEIFGFLSRIIFMFLIGLETDFPYILRNLRVAGIVACGGAAVGSVFGIAVS 130
           F Q  +A YY  F FL R  F+FLIGLE D  ++ RNL+ + ++  G   +  +  +   
Sbjct: 73  FLQKDSASYYIFFSFLLRTAFVFLIGLEIDLDFMKRNLKNSIVITLGSLVISGIIWLPFL 132

Query: 131 FFLYQQFEEKSSRFGFFFIVMLILAYTASPIVIRLAAELKFATSDVGKLAISSALINEMA 190
           +FL +  + K     F+   ++ L+ TA+P+VIR   + K  TS++G+LAIS  L  E+ 
Sbjct: 133 WFLIRFMQIKGDFLTFYLAFLITLSNTAAPVVIRSIIDWKLHTSEIGRLAISCGLFIEIT 192

Query: 191 CLAVFNAILALRSFQEFGKGIFCAVFIAGVVIL-NKYLASWFNKRNRNQKYLKNMEVFFL 250
            + ++  +L+  S       IF   F  GV+IL N++LASW  KRN  +KYL   E    
Sbjct: 193 NIFIYTIVLSFIS-GTMTADIFIYSFATGVIILTNRFLASWLPKRNPKEKYLSKAETLAF 252

Query: 251 LSLVIAASVIIELQAFNSIVSSFIFGVMFPKEGKSARTLMHKLTYSVHNFVLPIYFGYVG 310
           + L++  ++ IE    NS +  FI G+MFP+EGK+ RTL+ +L+Y +H FVLP+YFGY+G
Sbjct: 253 IILILIIALTIESSNLNSTLFVFIIGLMFPREGKTYRTLIQRLSYPIHEFVLPVYFGYIG 312

Query: 311 FQFDGNNLWKMSNVIIVGIMVLLSIGSKMSGTLAACNYLNIPLNEGVFLGFVLNLKGHAD 370
           F+F  N+L K  + +++G+ V LS+  K+ G L AC++L IP    +FL  +L++KGH  
Sbjct: 313 FRFSVNSLTK-RHYLVLGMTVALSLLGKLLGVLFACSFLKIPKQYWLFLSTMLSVKGHIG 372

Query: 371 LLLIGGASKSILTWSNPRAYNLLLISIVINTIISGPIVALLMRREHKLFSHAHTSLEYTD 430
           L+L+  ++     W  P  +++ + ++VI T++SG I +LL+R + K F+H  TSLE  D
Sbjct: 373 LVLL-DSNLMYKKWFTPVVHDMFVAALVIMTLLSGVITSLLLRSQEKSFAHIKTSLELFD 432

Query: 431 PTHELRALACAYGPRHLAGIFPLLSSLS----GGHTSQLSPFLLHLIELLHKRRTNVSYH 490
            T ELR L C YG RH  G   L+S+LS    G  +S  +P+L+HLI L  KR+T + YH
Sbjct: 433 TTEELRVLTCVYGVRHARGSISLVSALSGFSPGTSSSPFTPYLMHLIPLPKKRKTELLYH 492

Query: 491 ELEQD---ELSDDEGYGGNDVLEIHCAIDAFISDTKIFMSLSKAISAFPTLYEDVCNAAE 550
           EL++D       D+ +G N+ LEI+ +ID+F  D KI +   K ++    ++E++CNA E
Sbjct: 493 ELDEDAGNSNGGDDEFGTNEGLEINDSIDSFTRDRKIMVRQVKLVAPMENMHEEICNATE 552

Query: 551 DLRVSIVILPFHKHQRIDGKMESGKEGIRTTNQKILRHAPCSVGILVDRVQTGFLSFSHL 610
           DLRVSIV LPFHKHQRIDGK  +  E  R  N+K+L+ A CS+GI VDR  TG   F  L
Sbjct: 553 DLRVSIVFLPFHKHQRIDGKTTNDGEVFRHMNRKVLKQAQCSIGIFVDRNITG---FHQL 612

Query: 611 LVSDHVQHVATLFFGGPDDREALAWSRRMISHSRINLTVIRFV--PKATSDVEGTATTSS 670
             SD VQHVA LFFGGPDDREAL+  + + ++S+I+LTVI+FV     T  + G A T  
Sbjct: 613 HGSDSVQHVAALFFGGPDDREALSLCKWLTNNSQIHLTVIQFVADDSKTEKIVGDAVTKE 672

Query: 671 SDDGVLMALPSLRTTSSETDNTFLADFYDRHVSTGQVGYVEKQVKNGEETVAELRDIGDM 730
           +++ V + + S   T +ETD  FL +FY R V+TGQVG++EK+V NG +T+  LR+IG+M
Sbjct: 673 NNE-VFLEIVSEDQTENETDRIFLEEFYHRFVTTGQVGFIEKRVSNGMQTLTILREIGEM 732

Query: 731 YSLFIVGKGGRGHSPLTTGMSDWEECSELGTVGDLLASSDFNISGSVLIIQQHRHQKKDL 784
           YSLF+VGK  RG  P+T+GM+DWEEC ELGTVGD LASS+ +++ SVL++Q+HR+     
Sbjct: 733 YSLFVVGK-NRGDCPMTSGMNDWEECPELGTVGDFLASSNMDVNASVLVVQRHRNSFDSF 785

BLAST of CSPI03G47210 vs. ExPASy Swiss-Prot
Match: Q9SAK8 (Cation/H(+) antiporter 2 OS=Arabidopsis thaliana OX=3702 GN=CHX2 PE=2 SV=1)

HSP 1 Score: 610.1 bits (1572), Expect = 3.4e-173
Identity = 345/782 (44.12%), Postives = 491/782 (62.79%), Query Frame = 0

Query: 11  DDLFNPLSSMGVQVSFILVLSHFFHLVLKAFGQPGPIAQILAGMVLGPTGLSNIKAIRDV 70
           D+LFNPL++M +Q++ ILV S  F+L+LK  GQ GP+AQILAG+VL P  LS I  +++ 
Sbjct: 13  DELFNPLNTMFIQMACILVFSQLFYLLLKPCGQAGPVAQILAGIVLSPVLLSRIPKVKEF 72

Query: 71  FFQASAADYYEIFGFLSRIIFMFLIGLETDFPYILRNLRVAGIVACGGAAVGSVFGIAVS 130
           F Q +AADYY  F F  R  FMFLIGLE D  ++ RN + A ++      V  +   A  
Sbjct: 73  FLQKNAADYYSFFSFALRTSFMFLIGLEVDLHFMRRNFKKAAVITLSSFVVSGLLSFASL 132

Query: 131 FFLYQQFEEKSSRFGFFFIVMLILAYTASPIVIRLAAELKFATSDVGKLAISSALINEMA 190
                 F  K   F FF ++++ L+ TASP+V+R  A+ K  T ++G+L IS AL  E+ 
Sbjct: 133 MLFIPLFGIKEDYFTFFLVLLVTLSNTASPVVVRSIADWKLNTCEIGRLTISCALFIELT 192

Query: 191 CLAVFNAILALRSFQEFGKGIFCAVFIAGVVILNKYLASWFNKRNRNQKYLKNME--VFF 250
            + ++  I+A  S     +     +    ++++N  LA W  KRN  +KYL   E  VFF
Sbjct: 193 NVVLYTIIMAFISGTIILELFLFLLATVALILINMVLAPWLPKRNPKEKYLSKAETLVFF 252

Query: 251 LLSLVIAASVIIELQAFNSIVSSFIFGVMFPKEGKSARTLMHKLTYSVHNFVLPIYFGYV 310
           +  L+I   + IE    NS VS F  G+MFP++GK+ RTL+ +L+Y +H FVLP+YFGY+
Sbjct: 253 IFLLII--GITIESYDVNSSVSVFAIGIMFPRQGKTHRTLIQRLSYPIHEFVLPVYFGYI 312

Query: 311 GFQFDGNNLWKMSNVIIVGIMVLLSIGSKMSGTLAACNYLNIPLNEGVFLGFVLNLKGHA 370
           GF+F    L K   + IV I+V+++I  K  G ++AC YL IP    +FL  +L++KGH 
Sbjct: 313 GFRFSIIALTKRFYLGIV-IIVIVTIAGKFIGVISACMYLKIPKKYWLFLPTILSVKGHV 372

Query: 371 DLLLIGGASKSILTWSNPRAYNLLLISIVINTIISGPIVALLMRREHKLFSHAHTSLEYT 430
            LLL+  ++ S   W     +++++ ++VI T++SG + + L++   K F++  TSLE  
Sbjct: 373 GLLLL-DSNYSEKKWWTTTIHDMMVAALVITTLVSGVLASFLLKTREKDFAYEKTSLESH 432

Query: 431 DPTHELRALACAYGPRHLAGIFPLLSSLSG--GHTSQLSPFLLHLIELLHKRRTNVSYHE 490
           +   ELR L+CAYG RH  G   L+S+LSG  G +   +P L+HL+ L  KR++ + YHE
Sbjct: 433 NTNEELRILSCAYGVRHARGAISLVSALSGSRGASDPFTPLLMHLVPLPKKRKSELMYHE 492

Query: 491 LEQD--ELSDDEGYGGNDVLEIHCAIDAFISDTKIFMSLSKAISAFPTLYEDVCNAAEDL 550
            ++D    + D+ +G N+ LEI+ +ID+F  D+KI +   K ++    ++E++CNA EDL
Sbjct: 493 HDEDGGNANGDDEFGTNEGLEINDSIDSFAKDSKILIQQVKLVTQMLNMHEEICNATEDL 552

Query: 551 RVSIVILPFHKHQRIDGKMESGKEGIRTTNQKILRHAPCSVGILVDRVQTGFLSFSHLLV 610
           RVSIV LPFHKHQRIDGK  +  E  R  N+ +LRH PCS+GI VDR  TGF        
Sbjct: 553 RVSIVFLPFHKHQRIDGKTTNDGELFRQMNRNVLRHGPCSIGIFVDRNITGFQQPHGF-- 612

Query: 611 SDHVQHVATLFFGGPDDREALAWSRRMISHSRINLTVIRFV---PKATSDVEGTATTSSS 670
            D VQHVATLFFGGPDDREALA  R + +++ I+LTVI+FV    KA + V G A T  +
Sbjct: 613 -DSVQHVATLFFGGPDDREALALCRWLANNTLIHLTVIQFVSEESKAETPV-GNAMTRDN 672

Query: 671 DDGVLMALPSLRTTSSETDNTFLADFYDRHVSTGQVGYVEKQVKNGEETVAELRDIGDMY 730
           ++ V M +     T  ETD +FL +FY+R V+TGQVG++EK V NG  T+  LR+IG+MY
Sbjct: 673 NE-VFMEVLGRNQTEQETDRSFLEEFYNRFVTTGQVGFIEKLVSNGPHTLTILREIGEMY 732

Query: 731 SLFIVGKGGRGHSPLTTGMSDWEECSELGTVGDLLASSDFNISGSVLIIQQHRHQKKDLI 784
           SLF+VGK   G  P+T  M DWEEC ELGTVGD LASS  +++ SVL++Q+ RH     I
Sbjct: 733 SLFVVGK-STGDCPMTVRMKDWEECPELGTVGDFLASS-LDVNASVLVVQRQRHSHDSFI 783

BLAST of CSPI03G47210 vs. ExPASy Swiss-Prot
Match: Q9SIT5 (Cation/H(+) antiporter 15 OS=Arabidopsis thaliana OX=3702 GN=CHX15 PE=2 SV=1)

HSP 1 Score: 382.5 bits (981), Expect = 1.2e-104
Identity = 248/766 (32.38%), Postives = 407/766 (53.13%), Query Frame = 0

Query: 22  VQVSFILVLSHFFHLVLKAFGQPGPIAQILAGMVLGPTGLSNIKAIRDVFFQASAADYYE 81
           +Q++ ++V++ FF  +LK F QP  I++IL G+VLGP+ L          F   +    E
Sbjct: 42  LQLTLVVVVTRFFVFILKPFRQPRVISEILGGIVLGPSVLGRSTKFAHTIFPQRSVMVLE 101

Query: 82  IFGFLSRIIFMFLIGLETDFPYILRNLRVAGIVACGGAAVGSVFGIAVSFFLYQQFEEKS 141
               +  + F+FL+G+E D   + +  + A  +A GG  +  + G A SF +++  E+  
Sbjct: 102 TMANVGLLYFLFLVGVEMDIMVVRKTGKRALTIAIGGMVLPFLIGAAFSFSMHRS-EDHL 161

Query: 142 SRFGFFFIVMLILAYTASPIVIRLAAELKFATSDVGKLAISSALINEMACLAVFNAILAL 201
            +  +   + + L+ TA P++ R+ AELK   +++G++++S+AL+N+M    +    +AL
Sbjct: 162 GQGTYILFLGVALSVTAFPVLARILAELKLINTEIGRISMSAALVNDMFAWILLALAIAL 221

Query: 202 -----RSFQEFGKGIFCAVFIAGVVILNKYLASWFNKRNRNQKYLKNMEVFFLLSLVIAA 261
                 SF      I  AVFIA  V + +   +W  ++    +      +  +L+ V+ +
Sbjct: 222 AESDKTSFASLWVMISSAVFIAVCVFVVRPGIAWIIRKTPEGENFSEFHICLILTGVMIS 281

Query: 262 SVIIELQAFNSIVSSFIFGVMFPKEGKSARTLMHKLTYSVHNFVLPIYFGYVGFQFDGNN 321
             I +    +S+  +F+FG++ P  G    TL+ KL   V   +LP++F   G + +   
Sbjct: 282 GFITDAIGTHSVFGAFVFGLVIP-NGPLGLTLIEKLEDFVSGLLLPLFFAISGLKTNIAA 341

Query: 322 LWKMSNVIIVGIMVLLSIGSKMSGTLAACNYLNIPLNEGVFLGFVLNLKGHADLLLIG-G 381
           +   +  + + +++ L+   K+ GT+    +  +P+ EG+ LG +LN KG  +++++  G
Sbjct: 342 IQGPATWLTLFLVIFLACAGKVIGTVIVAFFHGMPVREGITLGLLLNTKGLVEMIVLNVG 401

Query: 382 ASKSILTWSNPRAYNLLLISIVINTIISGPIVALLMRREHKLFSHAHTSLEYTDPTHELR 441
             + +L   +     ++L+++V+  +I+ PIV +L +   K  S+   +++ T P  ELR
Sbjct: 402 KDQKVL--DDETFATMVLVALVMTGVIT-PIVTILYKPVKKSVSYKRRTIQQTKPDSELR 461

Query: 442 ALACAYGPRHLAGIFPLLSSLSGGHTSQLSPFLLHLIELLHKRRTNVSYHE--------L 501
            L C + PR++  I  LL +      S +  ++LHL+EL  +    +  H         L
Sbjct: 462 VLVCVHTPRNVPTIINLLEASHPTKRSPICIYVLHLVELTGRASAMLIVHNTRKSGRPAL 521

Query: 502 EQDELSDDEGYGGNDVLEIHCAIDAFISDTKIFMSLSKAISAFPTLYEDVCNAAEDLRVS 561
            + +   D      +  E H A  A    T        AIS + T++EDVC+ AED RVS
Sbjct: 522 NRTQAQSDHIINAFENYEQHAAFVAVQPLT--------AISPYSTMHEDVCSLAEDKRVS 581

Query: 562 IVILPFHKHQRIDGKMESGKEGIRTTNQKILRHAPCSVGILVDRVQTGFLSFSHLLVSDH 621
            +I+PFHK Q +DG MES     R  NQ +L ++PCSVGILVDR   G    +   VS  
Sbjct: 582 FIIIPFHKQQTVDGGMESTNPAYRLVNQNLLENSPCSVGILVDRGLNGATRLNSNTVS-- 641

Query: 622 VQHVATLFFGGPDDREALAWSRRMISHSRINLTVIRFVPKATSDVEGTATTSSSDDGVLM 681
              VA LFFGGPDDREALA++ RM  H  I LTV+RF+     D   TA+T +++D  L 
Sbjct: 642 -LQVAVLFFGGPDDREALAYAWRMAQHPGITLTVLRFI--HDEDEADTASTRATNDSDLK 701

Query: 682 ALPSLRTTSSETDNTFLADFYDRHVSTGQVGYVEKQVKNGEETVAELRDIGDMYSLFIVG 741
                     + D+ ++  F   +     + Y+EK V NGEETVA +R +   + LFIVG
Sbjct: 702 IPKMDHRKQRQLDDDYINLFRAENAEYESIVYIEKLVSNGEETVAAVRSMDSSHDLFIVG 761

Query: 742 KGGRGHSPLTTGMSDWEECSELGTVGDLLASSDFNISGSVLIIQQH 774
           +G    SPLT G++DW EC ELG +GDLLASSDF  + SVL++QQ+
Sbjct: 762 RGEGMSSPLTAGLTDWSECPELGAIGDLLASSDFAATVSVLVVQQY 789

BLAST of CSPI03G47210 vs. ExPASy Swiss-Prot
Match: Q9LUN4 (Cation/H(+) antiporter 19 OS=Arabidopsis thaliana OX=3702 GN=CHX19 PE=2 SV=1)

HSP 1 Score: 328.6 bits (841), Expect = 2.0e-88
Identity = 224/763 (29.36%), Postives = 390/763 (51.11%), Query Frame = 0

Query: 22  VQVSFILVLSHFFHLVLKAFGQPGPIAQILAGMVLGPTGLSNIKAIRDVFFQASAADYYE 81
           +Q+  ++V +      LK   QP  IA+I+ G++LGP+ L   KA  D  F   +    +
Sbjct: 37  LQIVLVVVFTRLLAYFLKPLKQPRVIAEIIGGILLGPSALGRSKAYLDTIFPKKSLTVLD 96

Query: 82  IFGFLSRIIFMFLIGLETDFPYILRNLRVAGIVACGGAAVGSVFGIAVSFFLYQQFEEKS 141
               +  + F+FL+GLE DF  I +  + + ++A  G ++  + G+  SF L     +  
Sbjct: 97  TLANIGLLFFLFLVGLELDFAAIKKTGKKSLLIAIAGISLPFIVGVGTSFVLSATISKGV 156

Query: 142 SRFGFFFIVMLILAYTASPIVIRLAAELKFATSDVGKLAISSALINEMACLAVFNAILAL 201
            +  F   + + L+ TA P++ R+ AELK  T+D+G++A+S+A +N++A   +    +AL
Sbjct: 157 DQLPFIVFMGVALSITAFPVLARILAELKLLTTDIGRMAMSAAGVNDVAAWILLALAIAL 216

Query: 202 RSFQEFGKG---------IFCAV-FIAGVVILNKYLASWFNKRNRNQKYLKNMEVFFLLS 261
                 G G         + C   F+   V+  K L ++  +R    + +K + V   L+
Sbjct: 217 S-----GDGTSPLVSVWVLLCGTGFVIFAVVAIKPLLAYMARRCPEGEPVKELYVCVTLT 276

Query: 262 LVIAASVIIELQAFNSIVSSFIFGVMFPKEGKSARTLMHKLTYSVHNFVLPIYFGYVGFQ 321
           +V+AAS + +    +++  +F+ G++ PKEG   R L  K+   V   +LP+YF   G +
Sbjct: 277 VVLAASFVTDTIGIHALFGAFVVGIVAPKEGPFCRILTEKIEDLVSGLLLPLYFAASGLK 336

Query: 322 FDGNNLWKMSNVIIVGIMVLLSIGSKMSGTLAACNYLNIPLNEGVFLGFVLNLKGHADLL 381
            D   +    +  ++ +++L +   K+ GT+ +     +P  E V LGF++N KG  +L+
Sbjct: 337 TDVTTIRGAQSWGLLVLVILTTCFGKIVGTVGSSMLCKVPFREAVTLGFLMNTKGLVELI 396

Query: 382 LIG-GASKSILTWSNPRAYNLLLISIVINTIISGPIVALLMRREHKLFSHAHTSLEYTDP 441
           ++  G  + +L   N +A+ +L++  +  T I+ PIV L+ +   K   + H +++  D 
Sbjct: 397 VLNIGKDRKVL---NDQAFAILVLMALFTTFITTPIVMLIYKPARKGAPYKHRTIQRKDH 456

Query: 442 THELRALACAYGPRHLAGIFPLLSSLSG-GHTSQLSPFLLHLIELLHKRRTNVSYHELEQ 501
             ELR LAC +  R++  +  L+ S  G G   +L  + +HL+EL  +       H+   
Sbjct: 457 DSELRILACFHSTRNIPTLINLIESSRGTGKKGRLCVYAMHLMELSERSSAIAMVHKARN 516

Query: 502 DELSD-DEGYGGNDVLEIHCAIDAFISDTKIFMSLSKAISAFPTLYEDVCNAAEDLRVSI 561
           + L   ++     D + I  A +A+     + +    AIS   +++ED+C +A   RV++
Sbjct: 517 NGLPIWNKIERSTDQMVI--AFEAYQHLRAVAVRPMTAISGLSSIHEDICTSAHQKRVAM 576

Query: 562 VILPFHKHQRIDGKMESGKEGIRTTNQKILRHAPCSVGILVDRVQTGFLSFSHLLVSDHV 621
           ++LPFHKHQR+DG MES        NQ++L+ APCSVGILVDR   G    S ++ S+  
Sbjct: 577 ILLPFHKHQRMDGAMESIGHRFHEVNQRVLQRAPCSVGILVDR---GLGGTSQVVASEVA 636

Query: 622 QHVATLFFGGPDDREALAWSRRMISHSRINLTVIRFVPKATSDVEGTATTSSSDDGVLMA 681
             V   FFGG DDREALA+  +M+ H  I LTV +FV        GT       +     
Sbjct: 637 YKVVIPFFGGLDDREALAYGMKMVEHPGITLTVYKFVA-----ARGTLKRFEKSEH---- 696

Query: 682 LPSLRTTSSETDNTFLADFYDRHVSTGQVGYVEKQVKNGEETVAELRDIGDMYSLFIVGK 741
                    ETD  F+ +  +       + Y E+ V++ ++ +A L+ +    +LF+VG+
Sbjct: 697 -DEKEKKEKETDEEFVRELMNDPRGNESLAYEERVVESKDDIIATLKSMSKC-NLFVVGR 756

Query: 742 GGRGHSPLTTGMSDWEECSELGTVGDLLASSDFNISGSVLIIQ 772
                S + +      +C ELG VG LL+SS+F+ + SVL++Q
Sbjct: 757 NAAVASLVKS-----TDCPELGPVGRLLSSSEFSTTASVLVVQ 770

BLAST of CSPI03G47210 vs. ExPASy Swiss-Prot
Match: Q9FFR9 (Cation/H(+) antiporter 18 OS=Arabidopsis thaliana OX=3702 GN=CHX18 PE=2 SV=1)

HSP 1 Score: 310.8 bits (795), Expect = 4.3e-83
Identity = 234/796 (29.40%), Postives = 393/796 (49.37%), Query Frame = 0

Query: 1   MDATHRMVCQDDLFNP----LSSMGVQVSFILVLSHFFHLVLKAFGQPGPIAQILAGMVL 60
           M AT   V Q D  NP    L    +Q+  ++VL+     +L+   QP  IA+++ G++L
Sbjct: 13  MKATSNGVFQGD--NPIDFALPLAILQIVIVIVLTRVLAYLLRPLRQPRVIAEVIGGIML 72

Query: 61  GPTGLSNIKAIRDVFFQASAADYYEIFGFLSRIIFMFLIGLETDFPYILRNLRVAGIVAC 120
           GP+ L   KA  D  F   +    E    L  + F+FL GLE D   + R  + A  +A 
Sbjct: 73  GPSLLGRSKAFLDAVFPKKSLTVLETLANLGLLFFLFLAGLEIDTKALRRTGKKALGIAL 132

Query: 121 GGAAVGSVFGIAVSFFLYQQFEEKSSRFGFFFIVMLILAYTASPIVIRLAAELKFATSDV 180
            G  +    GI  SF L     +  +   F   + + L+ TA P++ R+ AELK  T+++
Sbjct: 133 AGITLPFALGIGSSFVLKATISKGVNSTAFLVFMGVALSITAFPVLARILAELKLLTTEI 192

Query: 181 GKLAISSALINEMACLAVFNAILALR--------SFQEFGKGIFCAVFIAGVVILNKYLA 240
           G+LA+S+A +N++A   +    +AL         S   F  G  CA F+ G   +   + 
Sbjct: 193 GRLAMSAAAVNDVAAWILLALAIALSGSNTSPLVSLWVFLSG--CA-FVIGASFIIPPIF 252

Query: 241 SWFNKRNRNQKYLKNMEVFFLLSLVIAASVIIELQAFNSIVSSFIFGVMFPKEGKSARTL 300
            W ++R    + ++   +   L++V+    I +    +S+  +F+ GV+ PKEG  A  L
Sbjct: 253 RWISRRCHEGEPIEETYICATLAVVLVCGFITDAIGIHSMFGAFVVGVLIPKEGPFAGAL 312

Query: 301 MHKLTYSVHNFVLPIYFGYVGFQFDGNNLWKMSNVIIVGIMVLLSIGS---KMSGTLAAC 360
           + K+   V    LP+YF   G +    N+  +      G++VL++  +   K+ GTL   
Sbjct: 313 VEKVEDLVSGLFLPLYFVASGLK---TNVATIQGAQSWGLLVLVTATACFGKILGTLGVS 372

Query: 361 NYLNIPLNEGVFLGFVLNLKGHADLLLIG-GASKSILTWSNPRAYNLLLISIVINTIISG 420
               IP+ E + LGF++N KG  +L+++  G  + +L   N + + ++++  +  T I+ 
Sbjct: 373 LAFKIPMREAITLGFLMNTKGLVELIVLNIGKDRKVL---NDQTFAIMVLMALFTTFITT 432

Query: 421 PIVALL---MRREHKLFSHAHTSLEYTDPTHELRALACAYGPRHLAGIFPLLSSLSGGHT 480
           P+V  +    RR  K   + H ++E  +   +LR L C +G   +  +  LL +  G   
Sbjct: 433 PVVMAVYKPARRAKKEGEYKHRAVERENTNTQLRILTCFHGAGSIPSMINLLEASRGIEK 492

Query: 481 SQ-LSPFLLHLIELLHKRRTNVSYHELEQDELSDDEGYGGN-DVLEIHCAIDAFISDTKI 540
            + L  + LHL EL  +    +  H++ ++ +      G N D  ++  A  AF   +++
Sbjct: 493 GEGLCVYALHLRELSERSSAILMVHKVRKNGMPFWNRRGVNADADQVVVAFQAFQQLSRV 552

Query: 541 FMSLSKAISAFPTLYEDVCNAAEDLRVSIVILPFHKHQRIDGKMESGKEGIRTTNQKILR 600
            +    AIS+   ++ED+C  A   + +IVILPFHKHQ++DG +E+ +   R  N+++L 
Sbjct: 553 NVRPMTAISSMSDIHEDICTTAVRKKAAIVILPFHKHQQLDGSLETTRGDYRWVNRRVLL 612

Query: 601 HAPCSVGILVDRVQTGFLSFSHLLVSDHVQHVATLFFGGPDDREALAWSRRMISHSRINL 660
            APCSVGI VDR   G    S +   D    V  LFFGGPDDREALA+  RM  H  I L
Sbjct: 613 QAPCSVGIFVDR---GLGGSSQVSAQDVSYSVVVLFFGGPDDREALAYGLRMAEHPGIVL 672

Query: 661 TVIRFV--PKATSDVEGTATTSSSDDGVLMALPSLRTTSSETDNTFLADFYDRHVSTGQV 720
           TV RFV  P+   ++     ++++++       +    + ++D   +++          V
Sbjct: 673 TVFRFVVSPERVGEIVNVEVSNNNNE-------NQSVKNLKSDEEIMSEIRKISSVDESV 732

Query: 721 GYVEKQVKNGEETVAELRDIGDMYSLFIVGKGGRGHSPLTTGMSDWEECSELGTVGDLLA 774
            +VEKQ++N    V    +     +LF+VG+   G   +   + +  EC ELG VG LL 
Sbjct: 733 KFVEKQIENAAVDVRSAIEEVRRSNLFLVGRMPGGE--IALAIRENSECPELGPVGSLLI 785

BLAST of CSPI03G47210 vs. ExPASy TrEMBL
Match: A0A0A0LHF4 (Na_H_Exchanger domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G910760 PE=4 SV=1)

HSP 1 Score: 1526.1 bits (3950), Expect = 0.0e+00
Identity = 782/783 (99.87%), Postives = 782/783 (99.87%), Query Frame = 0

Query: 1   MDATHRMVCQDDLFNPLSSMGVQVSFILVLSHFFHLVLKAFGQPGPIAQILAGMVLGPTG 60
           MDATHRMVCQDDLFNPLSSMGVQVSFILVLSHFFHLVLKAFGQPGPIAQILAGMVLGPTG
Sbjct: 1   MDATHRMVCQDDLFNPLSSMGVQVSFILVLSHFFHLVLKAFGQPGPIAQILAGMVLGPTG 60

Query: 61  LSNIKAIRDVFFQASAADYYEIFGFLSRIIFMFLIGLETDFPYILRNLRVAGIVACGGAA 120
           LSNIKAIRDVFFQASAADYYEIFGFLSRIIFMFLIGLETDFPYILRNLRVAGIVACGGAA
Sbjct: 61  LSNIKAIRDVFFQASAADYYEIFGFLSRIIFMFLIGLETDFPYILRNLRVAGIVACGGAA 120

Query: 121 VGSVFGIAVSFFLYQQFEEKSSRFGFFFIVMLILAYTASPIVIRLAAELKFATSDVGKLA 180
           VGSVFGIAVSFFLYQQFEEKSSRFGFFFIVMLILAYTASPIVIRLAAELKFATSDVGKLA
Sbjct: 121 VGSVFGIAVSFFLYQQFEEKSSRFGFFFIVMLILAYTASPIVIRLAAELKFATSDVGKLA 180

Query: 181 ISSALINEMACLAVFNAILALRSFQEFGKGIFCAVFIAGVVILNKYLASWFNKRNRNQKY 240
           ISSALINEMACLAVFNAILALRSFQEFGKGIFCAVFIAGVVILNKYLASWFNKRNRNQKY
Sbjct: 181 ISSALINEMACLAVFNAILALRSFQEFGKGIFCAVFIAGVVILNKYLASWFNKRNRNQKY 240

Query: 241 LKNMEVFFLLSLVIAASVIIELQAFNSIVSSFIFGVMFPKEGKSARTLMHKLTYSVHNFV 300
           LKNMEVFFLLSLVIAASVIIELQAFNSIVSSFIFGVMFPKEGKSARTLMHKLTYSVHNFV
Sbjct: 241 LKNMEVFFLLSLVIAASVIIELQAFNSIVSSFIFGVMFPKEGKSARTLMHKLTYSVHNFV 300

Query: 301 LPIYFGYVGFQFDGNNLWKMSNVIIVGIMVLLSIGSKMSGTLAACNYLNIPLNEGVFLGF 360
           LPIYFGYVGFQFDGNNLWKMSNVIIVGIMVLLSIGSKMSGTLAACNYLNIPLNEGVFLGF
Sbjct: 301 LPIYFGYVGFQFDGNNLWKMSNVIIVGIMVLLSIGSKMSGTLAACNYLNIPLNEGVFLGF 360

Query: 361 VLNLKGHADLLLIGGASKSILTWSNPRAYNLLLISIVINTIISGPIVALLMRREHKLFSH 420
           VLNLKGHADLLLIGGASKSILTWSNPRAYNLLLISIVINTIISGPIVALLMRREHKLFSH
Sbjct: 361 VLNLKGHADLLLIGGASKSILTWSNPRAYNLLLISIVINTIISGPIVALLMRREHKLFSH 420

Query: 421 AHTSLEYTDPTHELRALACAYGPRHLAGIFPLLSSLSGGHTSQLSPFLLHLIELLHKRRT 480
           AHTSLEYTDPTHELRALACAYGPRHLAGIFPLLSSLSGGHTSQLSPFLLHLIELLHKRRT
Sbjct: 421 AHTSLEYTDPTHELRALACAYGPRHLAGIFPLLSSLSGGHTSQLSPFLLHLIELLHKRRT 480

Query: 481 NVSYHELEQDELSDDEGYGGNDVLEIHCAIDAFISDTKIFMSLSKAISAFPTLYEDVCNA 540
           NVSYHELEQDELSDDEGYGGNDVLEIHCAIDAFISDTKIFMSLSKAISAFPTLYEDVCNA
Sbjct: 481 NVSYHELEQDELSDDEGYGGNDVLEIHCAIDAFISDTKIFMSLSKAISAFPTLYEDVCNA 540

Query: 541 AEDLRVSIVILPFHKHQRIDGKMESGKEGIRTTNQKILRHAPCSVGILVDRVQTGFLSFS 600
           AEDLRVSIVILPFHKHQRIDGKMESGKEGIRTTNQKILRHAPCSVGILVDRVQTGFLSFS
Sbjct: 541 AEDLRVSIVILPFHKHQRIDGKMESGKEGIRTTNQKILRHAPCSVGILVDRVQTGFLSFS 600

Query: 601 HLLVSDHVQHVATLFFGGPDDREALAWSRRMISHSRINLTVIRFVPKATSDVEGTATTSS 660
           HLLVSDHVQHVATLFFGGPDDREALAWSRRMISHSRINLTVIRFVPKATSDVEG ATTSS
Sbjct: 601 HLLVSDHVQHVATLFFGGPDDREALAWSRRMISHSRINLTVIRFVPKATSDVEGAATTSS 660

Query: 661 SDDGVLMALPSLRTTSSETDNTFLADFYDRHVSTGQVGYVEKQVKNGEETVAELRDIGDM 720
           SDDGVLMALPSLRTTSSETDNTFLADFYDRHVSTGQVGYVEKQVKNGEETVAELRDIGDM
Sbjct: 661 SDDGVLMALPSLRTTSSETDNTFLADFYDRHVSTGQVGYVEKQVKNGEETVAELRDIGDM 720

Query: 721 YSLFIVGKGGRGHSPLTTGMSDWEECSELGTVGDLLASSDFNISGSVLIIQQHRHQKKDL 780
           YSLFIVGKGGRGHSPLTTGMSDWEECSELGTVGDLLASSDFNISGSVLIIQQHRHQKKDL
Sbjct: 721 YSLFIVGKGGRGHSPLTTGMSDWEECSELGTVGDLLASSDFNISGSVLIIQQHRHQKKDL 780

Query: 781 IDD 784
           IDD
Sbjct: 781 IDD 783

BLAST of CSPI03G47210 vs. ExPASy TrEMBL
Match: A0A1S3CRQ9 (cation/H(+) antiporter 1-like OS=Cucumis melo OX=3656 GN=LOC103504000 PE=4 SV=1)

HSP 1 Score: 1477.2 bits (3823), Expect = 0.0e+00
Identity = 751/783 (95.91%), Postives = 770/783 (98.34%), Query Frame = 0

Query: 1   MDATHRMVCQDDLFNPLSSMGVQVSFILVLSHFFHLVLKAFGQPGPIAQILAGMVLGPTG 60
           MDATHRMVCQ+DLFNPLSSMGVQVSFILVLSHFFHLVLKAFGQPGPIAQILAGMVLGPTG
Sbjct: 1   MDATHRMVCQEDLFNPLSSMGVQVSFILVLSHFFHLVLKAFGQPGPIAQILAGMVLGPTG 60

Query: 61  LSNIKAIRDVFFQASAADYYEIFGFLSRIIFMFLIGLETDFPYILRNLRVAGIVACGGAA 120
           LSNIKA+RDVFFQASAADYYEIFGFLSRIIFMFLIGLETDFPY+LRNLRVAGIVACGGAA
Sbjct: 61  LSNIKAVRDVFFQASAADYYEIFGFLSRIIFMFLIGLETDFPYLLRNLRVAGIVACGGAA 120

Query: 121 VGSVFGIAVSFFLYQQFEEKSSRFGFFFIVMLILAYTASPIVIRLAAELKFATSDVGKLA 180
           VGSVFGIAVSFFLYQQFEEKSS+FGFFFI+MLIL+YTASPIVIRLAAELKFATSDVGKLA
Sbjct: 121 VGSVFGIAVSFFLYQQFEEKSSKFGFFFIIMLILSYTASPIVIRLAAELKFATSDVGKLA 180

Query: 181 ISSALINEMACLAVFNAILALRSFQEFGKGIFCAVFIAGVVILNKYLASWFNKRNRNQKY 240
           ISSALINEM+CLAVFNAILALRSF+ FGKGIFCAVFIA VVILNKYLASW NKRNRNQKY
Sbjct: 181 ISSALINEMSCLAVFNAILALRSFRGFGKGIFCAVFIASVVILNKYLASWLNKRNRNQKY 240

Query: 241 LKNMEVFFLLSLVIAASVIIELQAFNSIVSSFIFGVMFPKEGKSARTLMHKLTYSVHNFV 300
           LKNMEVFFLLSLVIAASVIIEL+AFNSIVSSFIFGVMFPKEGKSARTLMHKLTYSVHNF+
Sbjct: 241 LKNMEVFFLLSLVIAASVIIELEAFNSIVSSFIFGVMFPKEGKSARTLMHKLTYSVHNFI 300

Query: 301 LPIYFGYVGFQFDGNNLWKMSNVIIVGIMVLLSIGSKMSGTLAACNYLNIPLNEGVFLGF 360
           LPIYFGYVGFQFDGNNLWK+SNVIIVGIMVLLSIGSKMSGTLAACNYLNIPLNEGVFLGF
Sbjct: 301 LPIYFGYVGFQFDGNNLWKLSNVIIVGIMVLLSIGSKMSGTLAACNYLNIPLNEGVFLGF 360

Query: 361 VLNLKGHADLLLIGGASKSILTWSNPRAYNLLLISIVINTIISGPIVALLMRREHKLFSH 420
           VLNLKGHADLLLIGGASK+ILTWSNPRAYNLLLISIVINTIISGPIVALLMRREHKLFSH
Sbjct: 361 VLNLKGHADLLLIGGASKAILTWSNPRAYNLLLISIVINTIISGPIVALLMRREHKLFSH 420

Query: 421 AHTSLEYTDPTHELRALACAYGPRHLAGIFPLLSSLSGGHTSQLSPFLLHLIELLHKRRT 480
           AHTSLEYTDPTHELRALAC YGPRHL+G+FPLLSSLSGGHTSQLSPFLLHLIELLHKRRT
Sbjct: 421 AHTSLEYTDPTHELRALACVYGPRHLSGLFPLLSSLSGGHTSQLSPFLLHLIELLHKRRT 480

Query: 481 NVSYHELEQDELSDDEGYGGNDVLEIHCAIDAFISDTKIFMSLSKAISAFPTLYEDVCNA 540
           NVSYHELEQDELSDDEGYGGNDVLE+HCAIDAFISDTKIFMSLSKAISAFPTLYEDVCNA
Sbjct: 481 NVSYHELEQDELSDDEGYGGNDVLEVHCAIDAFISDTKIFMSLSKAISAFPTLYEDVCNA 540

Query: 541 AEDLRVSIVILPFHKHQRIDGKMESGKEGIRTTNQKILRHAPCSVGILVDRVQTGFLSFS 600
           AEDLRVSIVILPFHKHQRIDGKMESGKEGIRTTNQKILRHAPCSVGILVDRVQTGF SFS
Sbjct: 541 AEDLRVSIVILPFHKHQRIDGKMESGKEGIRTTNQKILRHAPCSVGILVDRVQTGFSSFS 600

Query: 601 HLLVSDHVQHVATLFFGGPDDREALAWSRRMISHSRINLTVIRFVPKATSDVEGTATTSS 660
           HLLVSDHVQHVATLFFGGPDDREALAWSRRMISHSRINLTVIRFVP AT+ ++  AT SS
Sbjct: 601 HLLVSDHVQHVATLFFGGPDDREALAWSRRMISHSRINLTVIRFVPTATT-IDAAATASS 660

Query: 661 SDDGVLMALPSLRTTSSETDNTFLADFYDRHVSTGQVGYVEKQVKNGEETVAELRDIGDM 720
           SDDGVLMALPSLRTTSSETDNTFLADFYDRHVSTGQVGYVEKQVKNGEETVAELRDIGDM
Sbjct: 661 SDDGVLMALPSLRTTSSETDNTFLADFYDRHVSTGQVGYVEKQVKNGEETVAELRDIGDM 720

Query: 721 YSLFIVGKGGRGHSPLTTGMSDWEECSELGTVGDLLASSDFNISGSVLIIQQHRHQKKDL 780
           YSLFIVGKGGRGHS LTTGMSDWEEC ELGTVGDLLASSDFNISGSVLI+QQHRHQKKDL
Sbjct: 721 YSLFIVGKGGRGHSTLTTGMSDWEECPELGTVGDLLASSDFNISGSVLIVQQHRHQKKDL 780

Query: 781 IDD 784
            DD
Sbjct: 781 TDD 782

BLAST of CSPI03G47210 vs. ExPASy TrEMBL
Match: A0A6J1EY98 (cation/H(+) antiporter 1-like OS=Cucurbita moschata OX=3662 GN=LOC111439593 PE=4 SV=1)

HSP 1 Score: 1429.8 bits (3700), Expect = 0.0e+00
Identity = 719/785 (91.59%), Postives = 761/785 (96.94%), Query Frame = 0

Query: 1   MDATHRMVCQDDLFNPLSSMGVQVSFILVLSHFFHLVLKAFGQPGPIAQILAGMVLGPTG 60
           MDATHRMVCQ+DLFNPLSSMGVQVSFILVLSHFFHLVLKAFGQPGPIAQILAGMVLGPTG
Sbjct: 1   MDATHRMVCQEDLFNPLSSMGVQVSFILVLSHFFHLVLKAFGQPGPIAQILAGMVLGPTG 60

Query: 61  LSNIKAIRDVFFQASAADYYEIFGFLSRIIFMFLIGLETDFPYILRNLRVAGIVACGGAA 120
           +SNI+AIRDVFFQASAADYYEIFGFLSRI+FMFLIGLETDFPYI+RNLRVAGIVACGGAA
Sbjct: 61  ISNIRAIRDVFFQASAADYYEIFGFLSRIVFMFLIGLETDFPYIVRNLRVAGIVACGGAA 120

Query: 121 VGSVFGIAVSFFLYQQFEEKSSRFGFFFIVMLILAYTASPIVIRLAAELKFATSDVGKLA 180
           VGSVFGIAVSFFLYQQFEEK S+FGFFFI+MLILAYTASPIVIRLAAELKFATSDVGKLA
Sbjct: 121 VGSVFGIAVSFFLYQQFEEKGSKFGFFFIIMLILAYTASPIVIRLAAELKFATSDVGKLA 180

Query: 181 ISSALINEMACLAVFNAILALRSFQEFGKGIFCAVFIAGVVILNKYLASWFNKRNRNQKY 240
           ISSALINEMACLA+FNAI+ALRSF+ FG+GIFC +FI GVV+LNKYLASWFNKRNRNQKY
Sbjct: 181 ISSALINEMACLALFNAIVALRSFRGFGRGIFCTLFITGVVVLNKYLASWFNKRNRNQKY 240

Query: 241 LKNMEVFFLLSLVIAASVIIELQAFNSIVSSFIFGVMFPKEGKSARTLMHKLTYSVHNFV 300
           LKNMEVFFLLSLVIAASVIIEL+ FNSIV SF+ GVMFPKEGK+ARTL+HKLTYSVHNFV
Sbjct: 241 LKNMEVFFLLSLVIAASVIIELETFNSIVCSFVLGVMFPKEGKTARTLLHKLTYSVHNFV 300

Query: 301 LPIYFGYVGFQFDGNNLWKMSNVIIVGIMVLLSIGSKMSGTLAACNYLNIPLNEGVFLGF 360
           LPIYFGYVGFQFDGNNLWK+SNVIIV IMVLLSIGSKMSGTLAACNYLNIPLNEGVFLGF
Sbjct: 301 LPIYFGYVGFQFDGNNLWKLSNVIIVAIMVLLSIGSKMSGTLAACNYLNIPLNEGVFLGF 360

Query: 361 VLNLKGHADLLLIGGASKSILTWSNPRAYNLLLISIVINTIISGPIVALLMRREHKLFSH 420
           VLNLKGHADLLLIGGASKS+++WSNPRAYNLLLISIVINTIISGPIVALLMRRE+KLFSH
Sbjct: 361 VLNLKGHADLLLIGGASKSLMSWSNPRAYNLLLISIVINTIISGPIVALLMRREYKLFSH 420

Query: 421 AHTSLEYTDPTHELRALACAYGPRHLAGIFPLLSSLSGGHTSQLSPFLLHLIELLHKRRT 480
            HTSLE+TDP HELRALACAYGPRHL+G+FPLLS+LSGGHTS LSPFLLHLIELLHKRRT
Sbjct: 421 THTSLEHTDPNHELRALACAYGPRHLSGLFPLLSALSGGHTSHLSPFLLHLIELLHKRRT 480

Query: 481 NVSYHELEQDELSDDEGYGGNDVLEIHCAIDAFISDTKIFMSLSKAISAFPTLYEDVCNA 540
           N+SYHELEQDELSDDEGYGGNDVLEIHCAIDAFISDTKIFM LSKA+S+FPTLYEDVCNA
Sbjct: 481 NLSYHELEQDELSDDEGYGGNDVLEIHCAIDAFISDTKIFMPLSKAVSSFPTLYEDVCNA 540

Query: 541 AEDLRVSIVILPFHKHQRIDGKMESGKEGIRTTNQKILRHAPCSVGILVDRVQTGFLSFS 600
           AEDLRVSIVILPFHKHQRIDGKMESGKEGIRTTNQKILRHAPCSVGILVDR+QTGFL+FS
Sbjct: 541 AEDLRVSIVILPFHKHQRIDGKMESGKEGIRTTNQKILRHAPCSVGILVDRIQTGFLAFS 600

Query: 601 HLLVSDHVQHVATLFFGGPDDREALAWSRRMISHSRINLTVIRFVPKAT-SDVEG-TATT 660
           HLL+SDHVQHVATLFFGG DDREALAWSRRMISHSRINLTV+RFVP  T +DVEG T ++
Sbjct: 601 HLLISDHVQHVATLFFGGSDDREALAWSRRMISHSRINLTVVRFVPLVTINDVEGSTESS 660

Query: 661 SSSDDGVLMALPSLRTTSSETDNTFLADFYDRHVSTGQVGYVEKQVKNGEETVAELRDIG 720
           SSSD+GVLMALPSLR++SSETDN FLADFYDRHVSTGQ GYVEKQVKNG ETV ELRDIG
Sbjct: 661 SSSDEGVLMALPSLRSSSSETDNAFLADFYDRHVSTGQAGYVEKQVKNGTETVKELRDIG 720

Query: 721 DMYSLFIVGKGGRGHSPLTTGMSDWEECSELGTVGDLLASSDFNISGSVLIIQQHRHQKK 780
           DMYSLFIVGKGGRGHS LTTGMSDWEEC ELGTVGDLLASSDFNI+GSVL++QQHRHQKK
Sbjct: 721 DMYSLFIVGKGGRGHSTLTTGMSDWEECPELGTVGDLLASSDFNINGSVLVVQQHRHQKK 780

Query: 781 DLIDD 784
           DLIDD
Sbjct: 781 DLIDD 785

BLAST of CSPI03G47210 vs. ExPASy TrEMBL
Match: A0A6J1IDY5 (cation/H(+) antiporter 1-like OS=Cucurbita maxima OX=3661 GN=LOC111471794 PE=4 SV=1)

HSP 1 Score: 1428.7 bits (3697), Expect = 0.0e+00
Identity = 718/785 (91.46%), Postives = 761/785 (96.94%), Query Frame = 0

Query: 1   MDATHRMVCQDDLFNPLSSMGVQVSFILVLSHFFHLVLKAFGQPGPIAQILAGMVLGPTG 60
           MDATHRMVCQ+DLFNPLSSMGVQVSFILVLSHFFHLVLKAFGQPGPIAQILAGMVLGPTG
Sbjct: 1   MDATHRMVCQEDLFNPLSSMGVQVSFILVLSHFFHLVLKAFGQPGPIAQILAGMVLGPTG 60

Query: 61  LSNIKAIRDVFFQASAADYYEIFGFLSRIIFMFLIGLETDFPYILRNLRVAGIVACGGAA 120
           +SNI+AIRDVFFQASAADYYEIFGFLSRI+FMFLIGLETDFPYI+RNLRVAGIVACGGAA
Sbjct: 61  ISNIRAIRDVFFQASAADYYEIFGFLSRIVFMFLIGLETDFPYIIRNLRVAGIVACGGAA 120

Query: 121 VGSVFGIAVSFFLYQQFEEKSSRFGFFFIVMLILAYTASPIVIRLAAELKFATSDVGKLA 180
           VGSVFGIAVSFFLYQQFEEK S+FGFFFI+MLILAYTASPIVIRLAAELKFATSDVGKLA
Sbjct: 121 VGSVFGIAVSFFLYQQFEEKGSKFGFFFIIMLILAYTASPIVIRLAAELKFATSDVGKLA 180

Query: 181 ISSALINEMACLAVFNAILALRSFQEFGKGIFCAVFIAGVVILNKYLASWFNKRNRNQKY 240
           ISSALINEMACLA+FNAI+ALRSF+ FG+GIFC +FI GVVILNKYLASWFNKRNRNQKY
Sbjct: 181 ISSALINEMACLALFNAIVALRSFRGFGRGIFCTLFITGVVILNKYLASWFNKRNRNQKY 240

Query: 241 LKNMEVFFLLSLVIAASVIIELQAFNSIVSSFIFGVMFPKEGKSARTLMHKLTYSVHNFV 300
           LKNMEVFFLLSLVIAASVIIEL+ FNSIV SF+ GVMFPKEGK+ARTL+HKLTYSVHNFV
Sbjct: 241 LKNMEVFFLLSLVIAASVIIELETFNSIVCSFVLGVMFPKEGKTARTLLHKLTYSVHNFV 300

Query: 301 LPIYFGYVGFQFDGNNLWKMSNVIIVGIMVLLSIGSKMSGTLAACNYLNIPLNEGVFLGF 360
           LPIYFGYVGFQFDGNNLWK+SNVIIV IMVLLSIGSKMSGTLAACNYLNIPLNEGVFLGF
Sbjct: 301 LPIYFGYVGFQFDGNNLWKLSNVIIVAIMVLLSIGSKMSGTLAACNYLNIPLNEGVFLGF 360

Query: 361 VLNLKGHADLLLIGGASKSILTWSNPRAYNLLLISIVINTIISGPIVALLMRREHKLFSH 420
           VLNLKGHADLLLIGGASKS+++WSNPRAYNLLLISIVINTIISGPIVALLMRRE+KLFSH
Sbjct: 361 VLNLKGHADLLLIGGASKSLMSWSNPRAYNLLLISIVINTIISGPIVALLMRREYKLFSH 420

Query: 421 AHTSLEYTDPTHELRALACAYGPRHLAGIFPLLSSLSGGHTSQLSPFLLHLIELLHKRRT 480
            HTSLE+TDP HELRALACAYGPRHL+G+FPLLS+LSGGHTS LSPFLLHLIELLHKRRT
Sbjct: 421 THTSLEHTDPNHELRALACAYGPRHLSGLFPLLSALSGGHTSHLSPFLLHLIELLHKRRT 480

Query: 481 NVSYHELEQDELSDDEGYGGNDVLEIHCAIDAFISDTKIFMSLSKAISAFPTLYEDVCNA 540
           N+SYHELEQDELSDDEGYGGNDVLEIHCAIDAFISDTKIFM LSKA+S+FPTLYEDVCNA
Sbjct: 481 NLSYHELEQDELSDDEGYGGNDVLEIHCAIDAFISDTKIFMPLSKAVSSFPTLYEDVCNA 540

Query: 541 AEDLRVSIVILPFHKHQRIDGKMESGKEGIRTTNQKILRHAPCSVGILVDRVQTGFLSFS 600
           AEDLRVS+VILPFHKHQRIDGKMESGKEGIRTTNQKILRHAPCSVGILVDR+QTGFL+FS
Sbjct: 541 AEDLRVSVVILPFHKHQRIDGKMESGKEGIRTTNQKILRHAPCSVGILVDRIQTGFLAFS 600

Query: 601 HLLVSDHVQHVATLFFGGPDDREALAWSRRMISHSRINLTVIRFVPKAT-SDVEGTA-TT 660
           HLL+SDHVQHVATLFFGG DDREALAWSRRMISHSRINLTV+RFVP  T +D+E TA ++
Sbjct: 601 HLLISDHVQHVATLFFGGSDDREALAWSRRMISHSRINLTVVRFVPLVTINDMEATAESS 660

Query: 661 SSSDDGVLMALPSLRTTSSETDNTFLADFYDRHVSTGQVGYVEKQVKNGEETVAELRDIG 720
           SSSD+GVLMALPSLR++SSETDN FLADFYDRHVSTGQ GYVEKQVKNG ETV ELRDIG
Sbjct: 661 SSSDEGVLMALPSLRSSSSETDNAFLADFYDRHVSTGQAGYVEKQVKNGTETVKELRDIG 720

Query: 721 DMYSLFIVGKGGRGHSPLTTGMSDWEECSELGTVGDLLASSDFNISGSVLIIQQHRHQKK 780
           DMYSLFIVGKGGRGHS LTTGMSDWEEC ELGTVGDLLASSDFNI+GSVL++QQHRHQKK
Sbjct: 721 DMYSLFIVGKGGRGHSTLTTGMSDWEECPELGTVGDLLASSDFNINGSVLVVQQHRHQKK 780

Query: 781 DLIDD 784
           DLIDD
Sbjct: 781 DLIDD 785

BLAST of CSPI03G47210 vs. ExPASy TrEMBL
Match: A0A5D3E5E6 (Cation/H(+) antiporter 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G004000 PE=4 SV=1)

HSP 1 Score: 1385.2 bits (3584), Expect = 0.0e+00
Identity = 712/783 (90.93%), Postives = 728/783 (92.98%), Query Frame = 0

Query: 1   MDATHRMVCQDDLFNPLSSMGVQVSFILVLSHFFHLVLKAFGQPGPIAQILAGMVLGPTG 60
           MDATHRMVCQ+DLFNPLSSMGVQVSFILVLSHFFHLVLKAFGQPGPIAQILAGMVLGPTG
Sbjct: 1   MDATHRMVCQEDLFNPLSSMGVQVSFILVLSHFFHLVLKAFGQPGPIAQILAGMVLGPTG 60

Query: 61  LSNIKAIRDVFFQASAADYYEIFGFLSRIIFMFLIGLETDFPYILRNLRVAGIVACGGAA 120
           LSNIKA+RDVFFQASAADYYEIFGFLSRIIFMFLIGLETDFPY+LRNLRVAGIVACGGAA
Sbjct: 61  LSNIKAVRDVFFQASAADYYEIFGFLSRIIFMFLIGLETDFPYLLRNLRVAGIVACGGAA 120

Query: 121 VGSVFGIAVSFFLYQQFEEKSSRFGFFFIVMLILAYTASPIVIRLAAELKFATSDVGKLA 180
           VGSVFGIAVSFFLYQQFEEKSS+FGFFFI+MLIL+YTASPIVIRLAAELKFATSDVGKLA
Sbjct: 121 VGSVFGIAVSFFLYQQFEEKSSKFGFFFIIMLILSYTASPIVIRLAAELKFATSDVGKLA 180

Query: 181 ISSALINEMACLAVFNAILALRSFQEFGKGIFCAVFIAGVVILNKYLASWFNKRNRNQKY 240
           ISSALINEM+CLAVFNAILALRSF+ FGKGIFCAVFIA VVILNKYLASW NKRNRNQKY
Sbjct: 181 ISSALINEMSCLAVFNAILALRSFRGFGKGIFCAVFIASVVILNKYLASWLNKRNRNQKY 240

Query: 241 LKNMEVFFLLSLVIAASVIIELQAFNSIVSSFIFGVMFPKEGKSARTLMHKLTYSVHNFV 300
           LKNMEVFFLLSLVIAASVIIEL+AFNSIVSSFIFGVMFPKEGKSARTLMHKLTYSVHNF+
Sbjct: 241 LKNMEVFFLLSLVIAASVIIELEAFNSIVSSFIFGVMFPKEGKSARTLMHKLTYSVHNFI 300

Query: 301 LPIYFGYVGFQFDGNNLWKMSNVIIVGIMVLLSIGSKMSGTLAACNYLNIPLNEGVFLGF 360
           LPIYFGYVGFQFDGNNLWK+SNVIIVGIMVLLSIGSKMSGTLAACNYLNIPLNEGVFLGF
Sbjct: 301 LPIYFGYVGFQFDGNNLWKLSNVIIVGIMVLLSIGSKMSGTLAACNYLNIPLNEGVFLGF 360

Query: 361 VLNLKGHADLLLIGGASKSILTWSNPRAYNLLLISIVINTIISGPIVALLMRREHKLFSH 420
           VLNLKGHADLLLIGGASK+ILTWSNPRAYNLLLISIVINTIISGPIVALLMRREHKLFSH
Sbjct: 361 VLNLKGHADLLLIGGASKAILTWSNPRAYNLLLISIVINTIISGPIVALLMRREHKLFSH 420

Query: 421 AHTSLEYTDPTHELRALACAYGPRHLAGIFPLLSSLSGGHTSQLSPFLLHLIELLHKRRT 480
           AHTSLEYTDPTHELRALAC YGPRHL+G+FPLLSSLSGGHTSQLSPFLLHLIELLHKRRT
Sbjct: 421 AHTSLEYTDPTHELRALACVYGPRHLSGLFPLLSSLSGGHTSQLSPFLLHLIELLHKRRT 480

Query: 481 NVSYHELEQDELSDDEGYGGNDVLEIHCAIDAFISDTKIFMSLSKAISAFPTLYEDVCNA 540
           NVSYHELEQDELSDDEGYGGNDVLE+HCAIDAFISDTKIFMSLSKAISAFPTLYEDVCNA
Sbjct: 481 NVSYHELEQDELSDDEGYGGNDVLEVHCAIDAFISDTKIFMSLSKAISAFPTLYEDVCNA 540

Query: 541 AEDLRVSIVILPFHKHQRIDGKMESGKEGIRTTNQKILRHAPCSVGILVDRVQTGFLSFS 600
           AEDLRVSIVILPFHKHQRIDGKMESGKEGIRTTNQKILRHAPCSVGILVDRVQTGF SFS
Sbjct: 541 AEDLRVSIVILPFHKHQRIDGKMESGKEGIRTTNQKILRHAPCSVGILVDRVQTGFSSFS 600

Query: 601 HLLVSDHVQHVATLFFGGPDDREALAWSRRMISHSRINLTVIRFVPKATSDVEGTATTSS 660
           HLLVSDHVQHVATLFFGGPDDREALAWSRRMISHSRINLTVI                  
Sbjct: 601 HLLVSDHVQHVATLFFGGPDDREALAWSRRMISHSRINLTVI------------------ 660

Query: 661 SDDGVLMALPSLRTTSSETDNTFLADFYDRHVSTGQVGYVEKQVKNGEETVAELRDIGDM 720
                                        RHVSTGQVGYVEKQVKNGEETVAELRDIGDM
Sbjct: 661 -----------------------------RHVSTGQVGYVEKQVKNGEETVAELRDIGDM 720

Query: 721 YSLFIVGKGGRGHSPLTTGMSDWEECSELGTVGDLLASSDFNISGSVLIIQQHRHQKKDL 780
           YSLFIVGKGGRGHS LTTGMSDWEEC ELGTVGDLLASSDFNISGSVLI+QQHRHQKKDL
Sbjct: 721 YSLFIVGKGGRGHSTLTTGMSDWEECPELGTVGDLLASSDFNISGSVLIVQQHRHQKKDL 736

Query: 781 IDD 784
            DD
Sbjct: 781 TDD 736

BLAST of CSPI03G47210 vs. NCBI nr
Match: XP_031737603.1 (cation/H(+) antiporter 1 [Cucumis sativus])

HSP 1 Score: 1485.7 bits (3845), Expect = 0.0e+00
Identity = 763/764 (99.87%), Postives = 763/764 (99.87%), Query Frame = 0

Query: 20  MGVQVSFILVLSHFFHLVLKAFGQPGPIAQILAGMVLGPTGLSNIKAIRDVFFQASAADY 79
           MGVQVSFILVLSHFFHLVLKAFGQPGPIAQILAGMVLGPTGLSNIKAIRDVFFQASAADY
Sbjct: 1   MGVQVSFILVLSHFFHLVLKAFGQPGPIAQILAGMVLGPTGLSNIKAIRDVFFQASAADY 60

Query: 80  YEIFGFLSRIIFMFLIGLETDFPYILRNLRVAGIVACGGAAVGSVFGIAVSFFLYQQFEE 139
           YEIFGFLSRIIFMFLIGLETDFPYILRNLRVAGIVACGGAAVGSVFGIAVSFFLYQQFEE
Sbjct: 61  YEIFGFLSRIIFMFLIGLETDFPYILRNLRVAGIVACGGAAVGSVFGIAVSFFLYQQFEE 120

Query: 140 KSSRFGFFFIVMLILAYTASPIVIRLAAELKFATSDVGKLAISSALINEMACLAVFNAIL 199
           KSSRFGFFFIVMLILAYTASPIVIRLAAELKFATSDVGKLAISSALINEMACLAVFNAIL
Sbjct: 121 KSSRFGFFFIVMLILAYTASPIVIRLAAELKFATSDVGKLAISSALINEMACLAVFNAIL 180

Query: 200 ALRSFQEFGKGIFCAVFIAGVVILNKYLASWFNKRNRNQKYLKNMEVFFLLSLVIAASVI 259
           ALRSFQEFGKGIFCAVFIAGVVILNKYLASWFNKRNRNQKYLKNMEVFFLLSLVIAASVI
Sbjct: 181 ALRSFQEFGKGIFCAVFIAGVVILNKYLASWFNKRNRNQKYLKNMEVFFLLSLVIAASVI 240

Query: 260 IELQAFNSIVSSFIFGVMFPKEGKSARTLMHKLTYSVHNFVLPIYFGYVGFQFDGNNLWK 319
           IELQAFNSIVSSFIFGVMFPKEGKSARTLMHKLTYSVHNFVLPIYFGYVGFQFDGNNLWK
Sbjct: 241 IELQAFNSIVSSFIFGVMFPKEGKSARTLMHKLTYSVHNFVLPIYFGYVGFQFDGNNLWK 300

Query: 320 MSNVIIVGIMVLLSIGSKMSGTLAACNYLNIPLNEGVFLGFVLNLKGHADLLLIGGASKS 379
           MSNVIIVGIMVLLSIGSKMSGTLAACNYLNIPLNEGVFLGFVLNLKGHADLLLIGGASKS
Sbjct: 301 MSNVIIVGIMVLLSIGSKMSGTLAACNYLNIPLNEGVFLGFVLNLKGHADLLLIGGASKS 360

Query: 380 ILTWSNPRAYNLLLISIVINTIISGPIVALLMRREHKLFSHAHTSLEYTDPTHELRALAC 439
           ILTWSNPRAYNLLLISIVINTIISGPIVALLMRREHKLFSHAHTSLEYTDPTHELRALAC
Sbjct: 361 ILTWSNPRAYNLLLISIVINTIISGPIVALLMRREHKLFSHAHTSLEYTDPTHELRALAC 420

Query: 440 AYGPRHLAGIFPLLSSLSGGHTSQLSPFLLHLIELLHKRRTNVSYHELEQDELSDDEGYG 499
           AYGPRHLAGIFPLLSSLSGGHTSQLSPFLLHLIELLHKRRTNVSYHELEQDELSDDEGYG
Sbjct: 421 AYGPRHLAGIFPLLSSLSGGHTSQLSPFLLHLIELLHKRRTNVSYHELEQDELSDDEGYG 480

Query: 500 GNDVLEIHCAIDAFISDTKIFMSLSKAISAFPTLYEDVCNAAEDLRVSIVILPFHKHQRI 559
           GNDVLEIHCAIDAFISDTKIFMSLSKAISAFPTLYEDVCNAAEDLRVSIVILPFHKHQRI
Sbjct: 481 GNDVLEIHCAIDAFISDTKIFMSLSKAISAFPTLYEDVCNAAEDLRVSIVILPFHKHQRI 540

Query: 560 DGKMESGKEGIRTTNQKILRHAPCSVGILVDRVQTGFLSFSHLLVSDHVQHVATLFFGGP 619
           DGKMESGKEGIRTTNQKILRHAPCSVGILVDRVQTGFLSFSHLLVSDHVQHVATLFFGGP
Sbjct: 541 DGKMESGKEGIRTTNQKILRHAPCSVGILVDRVQTGFLSFSHLLVSDHVQHVATLFFGGP 600

Query: 620 DDREALAWSRRMISHSRINLTVIRFVPKATSDVEGTATTSSSDDGVLMALPSLRTTSSET 679
           DDREALAWSRRMISHSRINLTVIRFVPKATSDVEG ATTSSSDDGVLMALPSLRTTSSET
Sbjct: 601 DDREALAWSRRMISHSRINLTVIRFVPKATSDVEGAATTSSSDDGVLMALPSLRTTSSET 660

Query: 680 DNTFLADFYDRHVSTGQVGYVEKQVKNGEETVAELRDIGDMYSLFIVGKGGRGHSPLTTG 739
           DNTFLADFYDRHVSTGQVGYVEKQVKNGEETVAELRDIGDMYSLFIVGKGGRGHSPLTTG
Sbjct: 661 DNTFLADFYDRHVSTGQVGYVEKQVKNGEETVAELRDIGDMYSLFIVGKGGRGHSPLTTG 720

Query: 740 MSDWEECSELGTVGDLLASSDFNISGSVLIIQQHRHQKKDLIDD 784
           MSDWEECSELGTVGDLLASSDFNISGSVLIIQQHRHQKKDLIDD
Sbjct: 721 MSDWEECSELGTVGDLLASSDFNISGSVLIIQQHRHQKKDLIDD 764

BLAST of CSPI03G47210 vs. NCBI nr
Match: XP_008466643.2 (PREDICTED: cation/H(+) antiporter 1-like [Cucumis melo])

HSP 1 Score: 1477.2 bits (3823), Expect = 0.0e+00
Identity = 751/783 (95.91%), Postives = 770/783 (98.34%), Query Frame = 0

Query: 1   MDATHRMVCQDDLFNPLSSMGVQVSFILVLSHFFHLVLKAFGQPGPIAQILAGMVLGPTG 60
           MDATHRMVCQ+DLFNPLSSMGVQVSFILVLSHFFHLVLKAFGQPGPIAQILAGMVLGPTG
Sbjct: 1   MDATHRMVCQEDLFNPLSSMGVQVSFILVLSHFFHLVLKAFGQPGPIAQILAGMVLGPTG 60

Query: 61  LSNIKAIRDVFFQASAADYYEIFGFLSRIIFMFLIGLETDFPYILRNLRVAGIVACGGAA 120
           LSNIKA+RDVFFQASAADYYEIFGFLSRIIFMFLIGLETDFPY+LRNLRVAGIVACGGAA
Sbjct: 61  LSNIKAVRDVFFQASAADYYEIFGFLSRIIFMFLIGLETDFPYLLRNLRVAGIVACGGAA 120

Query: 121 VGSVFGIAVSFFLYQQFEEKSSRFGFFFIVMLILAYTASPIVIRLAAELKFATSDVGKLA 180
           VGSVFGIAVSFFLYQQFEEKSS+FGFFFI+MLIL+YTASPIVIRLAAELKFATSDVGKLA
Sbjct: 121 VGSVFGIAVSFFLYQQFEEKSSKFGFFFIIMLILSYTASPIVIRLAAELKFATSDVGKLA 180

Query: 181 ISSALINEMACLAVFNAILALRSFQEFGKGIFCAVFIAGVVILNKYLASWFNKRNRNQKY 240
           ISSALINEM+CLAVFNAILALRSF+ FGKGIFCAVFIA VVILNKYLASW NKRNRNQKY
Sbjct: 181 ISSALINEMSCLAVFNAILALRSFRGFGKGIFCAVFIASVVILNKYLASWLNKRNRNQKY 240

Query: 241 LKNMEVFFLLSLVIAASVIIELQAFNSIVSSFIFGVMFPKEGKSARTLMHKLTYSVHNFV 300
           LKNMEVFFLLSLVIAASVIIEL+AFNSIVSSFIFGVMFPKEGKSARTLMHKLTYSVHNF+
Sbjct: 241 LKNMEVFFLLSLVIAASVIIELEAFNSIVSSFIFGVMFPKEGKSARTLMHKLTYSVHNFI 300

Query: 301 LPIYFGYVGFQFDGNNLWKMSNVIIVGIMVLLSIGSKMSGTLAACNYLNIPLNEGVFLGF 360
           LPIYFGYVGFQFDGNNLWK+SNVIIVGIMVLLSIGSKMSGTLAACNYLNIPLNEGVFLGF
Sbjct: 301 LPIYFGYVGFQFDGNNLWKLSNVIIVGIMVLLSIGSKMSGTLAACNYLNIPLNEGVFLGF 360

Query: 361 VLNLKGHADLLLIGGASKSILTWSNPRAYNLLLISIVINTIISGPIVALLMRREHKLFSH 420
           VLNLKGHADLLLIGGASK+ILTWSNPRAYNLLLISIVINTIISGPIVALLMRREHKLFSH
Sbjct: 361 VLNLKGHADLLLIGGASKAILTWSNPRAYNLLLISIVINTIISGPIVALLMRREHKLFSH 420

Query: 421 AHTSLEYTDPTHELRALACAYGPRHLAGIFPLLSSLSGGHTSQLSPFLLHLIELLHKRRT 480
           AHTSLEYTDPTHELRALAC YGPRHL+G+FPLLSSLSGGHTSQLSPFLLHLIELLHKRRT
Sbjct: 421 AHTSLEYTDPTHELRALACVYGPRHLSGLFPLLSSLSGGHTSQLSPFLLHLIELLHKRRT 480

Query: 481 NVSYHELEQDELSDDEGYGGNDVLEIHCAIDAFISDTKIFMSLSKAISAFPTLYEDVCNA 540
           NVSYHELEQDELSDDEGYGGNDVLE+HCAIDAFISDTKIFMSLSKAISAFPTLYEDVCNA
Sbjct: 481 NVSYHELEQDELSDDEGYGGNDVLEVHCAIDAFISDTKIFMSLSKAISAFPTLYEDVCNA 540

Query: 541 AEDLRVSIVILPFHKHQRIDGKMESGKEGIRTTNQKILRHAPCSVGILVDRVQTGFLSFS 600
           AEDLRVSIVILPFHKHQRIDGKMESGKEGIRTTNQKILRHAPCSVGILVDRVQTGF SFS
Sbjct: 541 AEDLRVSIVILPFHKHQRIDGKMESGKEGIRTTNQKILRHAPCSVGILVDRVQTGFSSFS 600

Query: 601 HLLVSDHVQHVATLFFGGPDDREALAWSRRMISHSRINLTVIRFVPKATSDVEGTATTSS 660
           HLLVSDHVQHVATLFFGGPDDREALAWSRRMISHSRINLTVIRFVP AT+ ++  AT SS
Sbjct: 601 HLLVSDHVQHVATLFFGGPDDREALAWSRRMISHSRINLTVIRFVPTATT-IDAAATASS 660

Query: 661 SDDGVLMALPSLRTTSSETDNTFLADFYDRHVSTGQVGYVEKQVKNGEETVAELRDIGDM 720
           SDDGVLMALPSLRTTSSETDNTFLADFYDRHVSTGQVGYVEKQVKNGEETVAELRDIGDM
Sbjct: 661 SDDGVLMALPSLRTTSSETDNTFLADFYDRHVSTGQVGYVEKQVKNGEETVAELRDIGDM 720

Query: 721 YSLFIVGKGGRGHSPLTTGMSDWEECSELGTVGDLLASSDFNISGSVLIIQQHRHQKKDL 780
           YSLFIVGKGGRGHS LTTGMSDWEEC ELGTVGDLLASSDFNISGSVLI+QQHRHQKKDL
Sbjct: 721 YSLFIVGKGGRGHSTLTTGMSDWEECPELGTVGDLLASSDFNISGSVLIVQQHRHQKKDL 780

Query: 781 IDD 784
            DD
Sbjct: 781 TDD 782

BLAST of CSPI03G47210 vs. NCBI nr
Match: XP_022932972.1 (cation/H(+) antiporter 1-like [Cucurbita moschata])

HSP 1 Score: 1429.8 bits (3700), Expect = 0.0e+00
Identity = 719/785 (91.59%), Postives = 761/785 (96.94%), Query Frame = 0

Query: 1   MDATHRMVCQDDLFNPLSSMGVQVSFILVLSHFFHLVLKAFGQPGPIAQILAGMVLGPTG 60
           MDATHRMVCQ+DLFNPLSSMGVQVSFILVLSHFFHLVLKAFGQPGPIAQILAGMVLGPTG
Sbjct: 1   MDATHRMVCQEDLFNPLSSMGVQVSFILVLSHFFHLVLKAFGQPGPIAQILAGMVLGPTG 60

Query: 61  LSNIKAIRDVFFQASAADYYEIFGFLSRIIFMFLIGLETDFPYILRNLRVAGIVACGGAA 120
           +SNI+AIRDVFFQASAADYYEIFGFLSRI+FMFLIGLETDFPYI+RNLRVAGIVACGGAA
Sbjct: 61  ISNIRAIRDVFFQASAADYYEIFGFLSRIVFMFLIGLETDFPYIVRNLRVAGIVACGGAA 120

Query: 121 VGSVFGIAVSFFLYQQFEEKSSRFGFFFIVMLILAYTASPIVIRLAAELKFATSDVGKLA 180
           VGSVFGIAVSFFLYQQFEEK S+FGFFFI+MLILAYTASPIVIRLAAELKFATSDVGKLA
Sbjct: 121 VGSVFGIAVSFFLYQQFEEKGSKFGFFFIIMLILAYTASPIVIRLAAELKFATSDVGKLA 180

Query: 181 ISSALINEMACLAVFNAILALRSFQEFGKGIFCAVFIAGVVILNKYLASWFNKRNRNQKY 240
           ISSALINEMACLA+FNAI+ALRSF+ FG+GIFC +FI GVV+LNKYLASWFNKRNRNQKY
Sbjct: 181 ISSALINEMACLALFNAIVALRSFRGFGRGIFCTLFITGVVVLNKYLASWFNKRNRNQKY 240

Query: 241 LKNMEVFFLLSLVIAASVIIELQAFNSIVSSFIFGVMFPKEGKSARTLMHKLTYSVHNFV 300
           LKNMEVFFLLSLVIAASVIIEL+ FNSIV SF+ GVMFPKEGK+ARTL+HKLTYSVHNFV
Sbjct: 241 LKNMEVFFLLSLVIAASVIIELETFNSIVCSFVLGVMFPKEGKTARTLLHKLTYSVHNFV 300

Query: 301 LPIYFGYVGFQFDGNNLWKMSNVIIVGIMVLLSIGSKMSGTLAACNYLNIPLNEGVFLGF 360
           LPIYFGYVGFQFDGNNLWK+SNVIIV IMVLLSIGSKMSGTLAACNYLNIPLNEGVFLGF
Sbjct: 301 LPIYFGYVGFQFDGNNLWKLSNVIIVAIMVLLSIGSKMSGTLAACNYLNIPLNEGVFLGF 360

Query: 361 VLNLKGHADLLLIGGASKSILTWSNPRAYNLLLISIVINTIISGPIVALLMRREHKLFSH 420
           VLNLKGHADLLLIGGASKS+++WSNPRAYNLLLISIVINTIISGPIVALLMRRE+KLFSH
Sbjct: 361 VLNLKGHADLLLIGGASKSLMSWSNPRAYNLLLISIVINTIISGPIVALLMRREYKLFSH 420

Query: 421 AHTSLEYTDPTHELRALACAYGPRHLAGIFPLLSSLSGGHTSQLSPFLLHLIELLHKRRT 480
            HTSLE+TDP HELRALACAYGPRHL+G+FPLLS+LSGGHTS LSPFLLHLIELLHKRRT
Sbjct: 421 THTSLEHTDPNHELRALACAYGPRHLSGLFPLLSALSGGHTSHLSPFLLHLIELLHKRRT 480

Query: 481 NVSYHELEQDELSDDEGYGGNDVLEIHCAIDAFISDTKIFMSLSKAISAFPTLYEDVCNA 540
           N+SYHELEQDELSDDEGYGGNDVLEIHCAIDAFISDTKIFM LSKA+S+FPTLYEDVCNA
Sbjct: 481 NLSYHELEQDELSDDEGYGGNDVLEIHCAIDAFISDTKIFMPLSKAVSSFPTLYEDVCNA 540

Query: 541 AEDLRVSIVILPFHKHQRIDGKMESGKEGIRTTNQKILRHAPCSVGILVDRVQTGFLSFS 600
           AEDLRVSIVILPFHKHQRIDGKMESGKEGIRTTNQKILRHAPCSVGILVDR+QTGFL+FS
Sbjct: 541 AEDLRVSIVILPFHKHQRIDGKMESGKEGIRTTNQKILRHAPCSVGILVDRIQTGFLAFS 600

Query: 601 HLLVSDHVQHVATLFFGGPDDREALAWSRRMISHSRINLTVIRFVPKAT-SDVEG-TATT 660
           HLL+SDHVQHVATLFFGG DDREALAWSRRMISHSRINLTV+RFVP  T +DVEG T ++
Sbjct: 601 HLLISDHVQHVATLFFGGSDDREALAWSRRMISHSRINLTVVRFVPLVTINDVEGSTESS 660

Query: 661 SSSDDGVLMALPSLRTTSSETDNTFLADFYDRHVSTGQVGYVEKQVKNGEETVAELRDIG 720
           SSSD+GVLMALPSLR++SSETDN FLADFYDRHVSTGQ GYVEKQVKNG ETV ELRDIG
Sbjct: 661 SSSDEGVLMALPSLRSSSSETDNAFLADFYDRHVSTGQAGYVEKQVKNGTETVKELRDIG 720

Query: 721 DMYSLFIVGKGGRGHSPLTTGMSDWEECSELGTVGDLLASSDFNISGSVLIIQQHRHQKK 780
           DMYSLFIVGKGGRGHS LTTGMSDWEEC ELGTVGDLLASSDFNI+GSVL++QQHRHQKK
Sbjct: 721 DMYSLFIVGKGGRGHSTLTTGMSDWEECPELGTVGDLLASSDFNINGSVLVVQQHRHQKK 780

Query: 781 DLIDD 784
           DLIDD
Sbjct: 781 DLIDD 785

BLAST of CSPI03G47210 vs. NCBI nr
Match: KAG6571757.1 (Cation/H(+) antiporter 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7033619.1 Cation/H(+) antiporter 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1429.5 bits (3699), Expect = 0.0e+00
Identity = 719/785 (91.59%), Postives = 762/785 (97.07%), Query Frame = 0

Query: 1   MDATHRMVCQDDLFNPLSSMGVQVSFILVLSHFFHLVLKAFGQPGPIAQILAGMVLGPTG 60
           MDATHRMVCQ+DLFNPLSSMGVQVSFILVLSHFFHLVLKAFGQPGPIAQILAGMVLGPTG
Sbjct: 1   MDATHRMVCQEDLFNPLSSMGVQVSFILVLSHFFHLVLKAFGQPGPIAQILAGMVLGPTG 60

Query: 61  LSNIKAIRDVFFQASAADYYEIFGFLSRIIFMFLIGLETDFPYILRNLRVAGIVACGGAA 120
           +SNI+AIRDVFFQASAADYYEIFGFLSRI+FMFLIGLETDFPYI+RNLRVAGIVACGGAA
Sbjct: 61  ISNIRAIRDVFFQASAADYYEIFGFLSRIVFMFLIGLETDFPYIVRNLRVAGIVACGGAA 120

Query: 121 VGSVFGIAVSFFLYQQFEEKSSRFGFFFIVMLILAYTASPIVIRLAAELKFATSDVGKLA 180
           VGSVFGIAVSFFLYQQFEEK S+FGFFFI+MLILAYTASPIVIRLAAELKFATSDVGKLA
Sbjct: 121 VGSVFGIAVSFFLYQQFEEKGSKFGFFFIIMLILAYTASPIVIRLAAELKFATSDVGKLA 180

Query: 181 ISSALINEMACLAVFNAILALRSFQEFGKGIFCAVFIAGVVILNKYLASWFNKRNRNQKY 240
           ISSALINEMACLA+FNAI+ALRSF+ FG+GIFC +FI GVV+LNKYLASWFNKRNRNQKY
Sbjct: 181 ISSALINEMACLALFNAIVALRSFRGFGRGIFCTLFITGVVVLNKYLASWFNKRNRNQKY 240

Query: 241 LKNMEVFFLLSLVIAASVIIELQAFNSIVSSFIFGVMFPKEGKSARTLMHKLTYSVHNFV 300
           LKNMEVFFLLSLVIAASVIIEL+ FNSIV SF+ GVMFPKEGK+ARTL+HKLTYSVHNFV
Sbjct: 241 LKNMEVFFLLSLVIAASVIIELETFNSIVCSFVLGVMFPKEGKTARTLLHKLTYSVHNFV 300

Query: 301 LPIYFGYVGFQFDGNNLWKMSNVIIVGIMVLLSIGSKMSGTLAACNYLNIPLNEGVFLGF 360
           LPIYFGYVGFQFDGNNLWK+SNVIIV IMVLLSIGSKMSGTLAACNYLNIPLNEGVFLGF
Sbjct: 301 LPIYFGYVGFQFDGNNLWKLSNVIIVAIMVLLSIGSKMSGTLAACNYLNIPLNEGVFLGF 360

Query: 361 VLNLKGHADLLLIGGASKSILTWSNPRAYNLLLISIVINTIISGPIVALLMRREHKLFSH 420
           VLNLKGHADLLLIGGASKS+++WSNPRAYNLLLISIVINTIISGPIVALLMRRE+KLFSH
Sbjct: 361 VLNLKGHADLLLIGGASKSLMSWSNPRAYNLLLISIVINTIISGPIVALLMRREYKLFSH 420

Query: 421 AHTSLEYTDPTHELRALACAYGPRHLAGIFPLLSSLSGGHTSQLSPFLLHLIELLHKRRT 480
            HTSLE+TDP HELRALACAYGPRHL+G+FPLLS+LSGGHTS LSPFLLHLIELLHKRRT
Sbjct: 421 THTSLEHTDPHHELRALACAYGPRHLSGLFPLLSALSGGHTSHLSPFLLHLIELLHKRRT 480

Query: 481 NVSYHELEQDELSDDEGYGGNDVLEIHCAIDAFISDTKIFMSLSKAISAFPTLYEDVCNA 540
           N+SYHELEQDELSDDEGYGGNDVLEIHCAIDAFISDTKIFM LSKA+S+FPTLYEDVCNA
Sbjct: 481 NLSYHELEQDELSDDEGYGGNDVLEIHCAIDAFISDTKIFMPLSKAVSSFPTLYEDVCNA 540

Query: 541 AEDLRVSIVILPFHKHQRIDGKMESGKEGIRTTNQKILRHAPCSVGILVDRVQTGFLSFS 600
           AEDLRVSIVILPFHKHQRIDGKMESGKEGIRTTNQKILRHAPCSVGILVDR+QTGFL+FS
Sbjct: 541 AEDLRVSIVILPFHKHQRIDGKMESGKEGIRTTNQKILRHAPCSVGILVDRIQTGFLAFS 600

Query: 601 HLLVSDHVQHVATLFFGGPDDREALAWSRRMISHSRINLTVIRFVPKAT-SDVEGTA-TT 660
           HLL+SDHVQHVATLFFGG DDREALAWSRRMISHSRINLTV+RFVP  T +DVEG+A ++
Sbjct: 601 HLLISDHVQHVATLFFGGSDDREALAWSRRMISHSRINLTVVRFVPLVTLNDVEGSAESS 660

Query: 661 SSSDDGVLMALPSLRTTSSETDNTFLADFYDRHVSTGQVGYVEKQVKNGEETVAELRDIG 720
           SSSD+GVLMALPSLR++SSETDN FLADFYDRHVSTGQ GYVEKQVKNG ETV ELRDIG
Sbjct: 661 SSSDEGVLMALPSLRSSSSETDNAFLADFYDRHVSTGQAGYVEKQVKNGTETVKELRDIG 720

Query: 721 DMYSLFIVGKGGRGHSPLTTGMSDWEECSELGTVGDLLASSDFNISGSVLIIQQHRHQKK 780
           DMYSLFIVGKGGRGHS LTTGMSDWEEC ELGTVGDLLASSDFNI+GSVL++QQHRHQKK
Sbjct: 721 DMYSLFIVGKGGRGHSTLTTGMSDWEECPELGTVGDLLASSDFNINGSVLVVQQHRHQKK 780

Query: 781 DLIDD 784
           DLIDD
Sbjct: 781 DLIDD 785

BLAST of CSPI03G47210 vs. NCBI nr
Match: XP_022973229.1 (cation/H(+) antiporter 1-like [Cucurbita maxima])

HSP 1 Score: 1428.7 bits (3697), Expect = 0.0e+00
Identity = 718/785 (91.46%), Postives = 761/785 (96.94%), Query Frame = 0

Query: 1   MDATHRMVCQDDLFNPLSSMGVQVSFILVLSHFFHLVLKAFGQPGPIAQILAGMVLGPTG 60
           MDATHRMVCQ+DLFNPLSSMGVQVSFILVLSHFFHLVLKAFGQPGPIAQILAGMVLGPTG
Sbjct: 1   MDATHRMVCQEDLFNPLSSMGVQVSFILVLSHFFHLVLKAFGQPGPIAQILAGMVLGPTG 60

Query: 61  LSNIKAIRDVFFQASAADYYEIFGFLSRIIFMFLIGLETDFPYILRNLRVAGIVACGGAA 120
           +SNI+AIRDVFFQASAADYYEIFGFLSRI+FMFLIGLETDFPYI+RNLRVAGIVACGGAA
Sbjct: 61  ISNIRAIRDVFFQASAADYYEIFGFLSRIVFMFLIGLETDFPYIIRNLRVAGIVACGGAA 120

Query: 121 VGSVFGIAVSFFLYQQFEEKSSRFGFFFIVMLILAYTASPIVIRLAAELKFATSDVGKLA 180
           VGSVFGIAVSFFLYQQFEEK S+FGFFFI+MLILAYTASPIVIRLAAELKFATSDVGKLA
Sbjct: 121 VGSVFGIAVSFFLYQQFEEKGSKFGFFFIIMLILAYTASPIVIRLAAELKFATSDVGKLA 180

Query: 181 ISSALINEMACLAVFNAILALRSFQEFGKGIFCAVFIAGVVILNKYLASWFNKRNRNQKY 240
           ISSALINEMACLA+FNAI+ALRSF+ FG+GIFC +FI GVVILNKYLASWFNKRNRNQKY
Sbjct: 181 ISSALINEMACLALFNAIVALRSFRGFGRGIFCTLFITGVVILNKYLASWFNKRNRNQKY 240

Query: 241 LKNMEVFFLLSLVIAASVIIELQAFNSIVSSFIFGVMFPKEGKSARTLMHKLTYSVHNFV 300
           LKNMEVFFLLSLVIAASVIIEL+ FNSIV SF+ GVMFPKEGK+ARTL+HKLTYSVHNFV
Sbjct: 241 LKNMEVFFLLSLVIAASVIIELETFNSIVCSFVLGVMFPKEGKTARTLLHKLTYSVHNFV 300

Query: 301 LPIYFGYVGFQFDGNNLWKMSNVIIVGIMVLLSIGSKMSGTLAACNYLNIPLNEGVFLGF 360
           LPIYFGYVGFQFDGNNLWK+SNVIIV IMVLLSIGSKMSGTLAACNYLNIPLNEGVFLGF
Sbjct: 301 LPIYFGYVGFQFDGNNLWKLSNVIIVAIMVLLSIGSKMSGTLAACNYLNIPLNEGVFLGF 360

Query: 361 VLNLKGHADLLLIGGASKSILTWSNPRAYNLLLISIVINTIISGPIVALLMRREHKLFSH 420
           VLNLKGHADLLLIGGASKS+++WSNPRAYNLLLISIVINTIISGPIVALLMRRE+KLFSH
Sbjct: 361 VLNLKGHADLLLIGGASKSLMSWSNPRAYNLLLISIVINTIISGPIVALLMRREYKLFSH 420

Query: 421 AHTSLEYTDPTHELRALACAYGPRHLAGIFPLLSSLSGGHTSQLSPFLLHLIELLHKRRT 480
            HTSLE+TDP HELRALACAYGPRHL+G+FPLLS+LSGGHTS LSPFLLHLIELLHKRRT
Sbjct: 421 THTSLEHTDPNHELRALACAYGPRHLSGLFPLLSALSGGHTSHLSPFLLHLIELLHKRRT 480

Query: 481 NVSYHELEQDELSDDEGYGGNDVLEIHCAIDAFISDTKIFMSLSKAISAFPTLYEDVCNA 540
           N+SYHELEQDELSDDEGYGGNDVLEIHCAIDAFISDTKIFM LSKA+S+FPTLYEDVCNA
Sbjct: 481 NLSYHELEQDELSDDEGYGGNDVLEIHCAIDAFISDTKIFMPLSKAVSSFPTLYEDVCNA 540

Query: 541 AEDLRVSIVILPFHKHQRIDGKMESGKEGIRTTNQKILRHAPCSVGILVDRVQTGFLSFS 600
           AEDLRVS+VILPFHKHQRIDGKMESGKEGIRTTNQKILRHAPCSVGILVDR+QTGFL+FS
Sbjct: 541 AEDLRVSVVILPFHKHQRIDGKMESGKEGIRTTNQKILRHAPCSVGILVDRIQTGFLAFS 600

Query: 601 HLLVSDHVQHVATLFFGGPDDREALAWSRRMISHSRINLTVIRFVPKAT-SDVEGTA-TT 660
           HLL+SDHVQHVATLFFGG DDREALAWSRRMISHSRINLTV+RFVP  T +D+E TA ++
Sbjct: 601 HLLISDHVQHVATLFFGGSDDREALAWSRRMISHSRINLTVVRFVPLVTINDMEATAESS 660

Query: 661 SSSDDGVLMALPSLRTTSSETDNTFLADFYDRHVSTGQVGYVEKQVKNGEETVAELRDIG 720
           SSSD+GVLMALPSLR++SSETDN FLADFYDRHVSTGQ GYVEKQVKNG ETV ELRDIG
Sbjct: 661 SSSDEGVLMALPSLRSSSSETDNAFLADFYDRHVSTGQAGYVEKQVKNGTETVKELRDIG 720

Query: 721 DMYSLFIVGKGGRGHSPLTTGMSDWEECSELGTVGDLLASSDFNISGSVLIIQQHRHQKK 780
           DMYSLFIVGKGGRGHS LTTGMSDWEEC ELGTVGDLLASSDFNI+GSVL++QQHRHQKK
Sbjct: 721 DMYSLFIVGKGGRGHSTLTTGMSDWEECPELGTVGDLLASSDFNINGSVLVVQQHRHQKK 780

Query: 781 DLIDD 784
           DLIDD
Sbjct: 781 DLIDD 785

BLAST of CSPI03G47210 vs. TAIR 10
Match: AT1G16380.1 (Cation/hydrogen exchanger family protein )

HSP 1 Score: 629.4 bits (1622), Expect = 3.9e-180
Identity = 347/783 (44.32%), Postives = 506/783 (64.62%), Query Frame = 0

Query: 11  DDLFNPLSSMGVQVSFILVLSHFFHLVLKAFGQPGPIAQILAGMVLGPTGLSNIKAIRDV 70
           D LFNPL++M +Q++ ILV S FF+L LK  GQ GP+AQILAG+VL  + L+ I+ + + 
Sbjct: 13  DALFNPLNTMFIQMACILVFSQFFYLFLKPCGQAGPVAQILAGIVL--SLLTIIRKVHEF 72

Query: 71  FFQASAADYYEIFGFLSRIIFMFLIGLETDFPYILRNLRVAGIVACGGAAVGSVFGIAVS 130
           F Q  +A YY  F FL R  F+FLIGLE D  ++ RNL+ + ++  G   +  +  +   
Sbjct: 73  FLQKDSASYYIFFSFLLRTAFVFLIGLEIDLDFMKRNLKNSIVITLGSLVISGIIWLPFL 132

Query: 131 FFLYQQFEEKSSRFGFFFIVMLILAYTASPIVIRLAAELKFATSDVGKLAISSALINEMA 190
           +FL +  + K     F+   ++ L+ TA+P+VIR   + K  TS++G+LAIS  L  E+ 
Sbjct: 133 WFLIRFMQIKGDFLTFYLAFLITLSNTAAPVVIRSIIDWKLHTSEIGRLAISCGLFIEIT 192

Query: 191 CLAVFNAILALRSFQEFGKGIFCAVFIAGVVIL-NKYLASWFNKRNRNQKYLKNMEVFFL 250
            + ++  +L+  S       IF   F  GV+IL N++LASW  KRN  +KYL   E    
Sbjct: 193 NIFIYTIVLSFIS-GTMTADIFIYSFATGVIILTNRFLASWLPKRNPKEKYLSKAETLAF 252

Query: 251 LSLVIAASVIIELQAFNSIVSSFIFGVMFPKEGKSARTLMHKLTYSVHNFVLPIYFGYVG 310
           + L++  ++ IE    NS +  FI G+MFP+EGK+ RTL+ +L+Y +H FVLP+YFGY+G
Sbjct: 253 IILILIIALTIESSNLNSTLFVFIIGLMFPREGKTYRTLIQRLSYPIHEFVLPVYFGYIG 312

Query: 311 FQFDGNNLWKMSNVIIVGIMVLLSIGSKMSGTLAACNYLNIPLNEGVFLGFVLNLKGHAD 370
           F+F  N+L K  + +++G+ V LS+  K+ G L AC++L IP    +FL  +L++KGH  
Sbjct: 313 FRFSVNSLTK-RHYLVLGMTVALSLLGKLLGVLFACSFLKIPKQYWLFLSTMLSVKGHIG 372

Query: 371 LLLIGGASKSILTWSNPRAYNLLLISIVINTIISGPIVALLMRREHKLFSHAHTSLEYTD 430
           L+L+  ++     W  P  +++ + ++VI T++SG I +LL+R + K F+H  TSLE  D
Sbjct: 373 LVLL-DSNLMYKKWFTPVVHDMFVAALVIMTLLSGVITSLLLRSQEKSFAHIKTSLELFD 432

Query: 431 PTHELRALACAYGPRHLAGIFPLLSSLS----GGHTSQLSPFLLHLIELLHKRRTNVSYH 490
            T ELR L C YG RH  G   L+S+LS    G  +S  +P+L+HLI L  KR+T + YH
Sbjct: 433 TTEELRVLTCVYGVRHARGSISLVSALSGFSPGTSSSPFTPYLMHLIPLPKKRKTELLYH 492

Query: 491 ELEQD---ELSDDEGYGGNDVLEIHCAIDAFISDTKIFMSLSKAISAFPTLYEDVCNAAE 550
           EL++D       D+ +G N+ LEI+ +ID+F  D KI +   K ++    ++E++CNA E
Sbjct: 493 ELDEDAGNSNGGDDEFGTNEGLEINDSIDSFTRDRKIMVRQVKLVAPMENMHEEICNATE 552

Query: 551 DLRVSIVILPFHKHQRIDGKMESGKEGIRTTNQKILRHAPCSVGILVDRVQTGFLSFSHL 610
           DLRVSIV LPFHKHQRIDGK  +  E  R  N+K+L+ A CS+GI VDR  TG   F  L
Sbjct: 553 DLRVSIVFLPFHKHQRIDGKTTNDGEVFRHMNRKVLKQAQCSIGIFVDRNITG---FHQL 612

Query: 611 LVSDHVQHVATLFFGGPDDREALAWSRRMISHSRINLTVIRFV--PKATSDVEGTATTSS 670
             SD VQHVA LFFGGPDDREAL+  + + ++S+I+LTVI+FV     T  + G A T  
Sbjct: 613 HGSDSVQHVAALFFGGPDDREALSLCKWLTNNSQIHLTVIQFVADDSKTEKIVGDAVTKE 672

Query: 671 SDDGVLMALPSLRTTSSETDNTFLADFYDRHVSTGQVGYVEKQVKNGEETVAELRDIGDM 730
           +++ V + + S   T +ETD  FL +FY R V+TGQVG++EK+V NG +T+  LR+IG+M
Sbjct: 673 NNE-VFLEIVSEDQTENETDRIFLEEFYHRFVTTGQVGFIEKRVSNGMQTLTILREIGEM 732

Query: 731 YSLFIVGKGGRGHSPLTTGMSDWEECSELGTVGDLLASSDFNISGSVLIIQQHRHQKKDL 784
           YSLF+VGK  RG  P+T+GM+DWEEC ELGTVGD LASS+ +++ SVL++Q+HR+     
Sbjct: 733 YSLFVVGK-NRGDCPMTSGMNDWEECPELGTVGDFLASSNMDVNASVLVVQRHRNSFDSF 785

BLAST of CSPI03G47210 vs. TAIR 10
Match: AT1G79400.1 (cation/H+ exchanger 2 )

HSP 1 Score: 610.1 bits (1572), Expect = 2.4e-174
Identity = 345/782 (44.12%), Postives = 491/782 (62.79%), Query Frame = 0

Query: 11  DDLFNPLSSMGVQVSFILVLSHFFHLVLKAFGQPGPIAQILAGMVLGPTGLSNIKAIRDV 70
           D+LFNPL++M +Q++ ILV S  F+L+LK  GQ GP+AQILAG+VL P  LS I  +++ 
Sbjct: 13  DELFNPLNTMFIQMACILVFSQLFYLLLKPCGQAGPVAQILAGIVLSPVLLSRIPKVKEF 72

Query: 71  FFQASAADYYEIFGFLSRIIFMFLIGLETDFPYILRNLRVAGIVACGGAAVGSVFGIAVS 130
           F Q +AADYY  F F  R  FMFLIGLE D  ++ RN + A ++      V  +   A  
Sbjct: 73  FLQKNAADYYSFFSFALRTSFMFLIGLEVDLHFMRRNFKKAAVITLSSFVVSGLLSFASL 132

Query: 131 FFLYQQFEEKSSRFGFFFIVMLILAYTASPIVIRLAAELKFATSDVGKLAISSALINEMA 190
                 F  K   F FF ++++ L+ TASP+V+R  A+ K  T ++G+L IS AL  E+ 
Sbjct: 133 MLFIPLFGIKEDYFTFFLVLLVTLSNTASPVVVRSIADWKLNTCEIGRLTISCALFIELT 192

Query: 191 CLAVFNAILALRSFQEFGKGIFCAVFIAGVVILNKYLASWFNKRNRNQKYLKNME--VFF 250
            + ++  I+A  S     +     +    ++++N  LA W  KRN  +KYL   E  VFF
Sbjct: 193 NVVLYTIIMAFISGTIILELFLFLLATVALILINMVLAPWLPKRNPKEKYLSKAETLVFF 252

Query: 251 LLSLVIAASVIIELQAFNSIVSSFIFGVMFPKEGKSARTLMHKLTYSVHNFVLPIYFGYV 310
           +  L+I   + IE    NS VS F  G+MFP++GK+ RTL+ +L+Y +H FVLP+YFGY+
Sbjct: 253 IFLLII--GITIESYDVNSSVSVFAIGIMFPRQGKTHRTLIQRLSYPIHEFVLPVYFGYI 312

Query: 311 GFQFDGNNLWKMSNVIIVGIMVLLSIGSKMSGTLAACNYLNIPLNEGVFLGFVLNLKGHA 370
           GF+F    L K   + IV I+V+++I  K  G ++AC YL IP    +FL  +L++KGH 
Sbjct: 313 GFRFSIIALTKRFYLGIV-IIVIVTIAGKFIGVISACMYLKIPKKYWLFLPTILSVKGHV 372

Query: 371 DLLLIGGASKSILTWSNPRAYNLLLISIVINTIISGPIVALLMRREHKLFSHAHTSLEYT 430
            LLL+  ++ S   W     +++++ ++VI T++SG + + L++   K F++  TSLE  
Sbjct: 373 GLLLL-DSNYSEKKWWTTTIHDMMVAALVITTLVSGVLASFLLKTREKDFAYEKTSLESH 432

Query: 431 DPTHELRALACAYGPRHLAGIFPLLSSLSG--GHTSQLSPFLLHLIELLHKRRTNVSYHE 490
           +   ELR L+CAYG RH  G   L+S+LSG  G +   +P L+HL+ L  KR++ + YHE
Sbjct: 433 NTNEELRILSCAYGVRHARGAISLVSALSGSRGASDPFTPLLMHLVPLPKKRKSELMYHE 492

Query: 491 LEQD--ELSDDEGYGGNDVLEIHCAIDAFISDTKIFMSLSKAISAFPTLYEDVCNAAEDL 550
            ++D    + D+ +G N+ LEI+ +ID+F  D+KI +   K ++    ++E++CNA EDL
Sbjct: 493 HDEDGGNANGDDEFGTNEGLEINDSIDSFAKDSKILIQQVKLVTQMLNMHEEICNATEDL 552

Query: 551 RVSIVILPFHKHQRIDGKMESGKEGIRTTNQKILRHAPCSVGILVDRVQTGFLSFSHLLV 610
           RVSIV LPFHKHQRIDGK  +  E  R  N+ +LRH PCS+GI VDR  TGF        
Sbjct: 553 RVSIVFLPFHKHQRIDGKTTNDGELFRQMNRNVLRHGPCSIGIFVDRNITGFQQPHGF-- 612

Query: 611 SDHVQHVATLFFGGPDDREALAWSRRMISHSRINLTVIRFV---PKATSDVEGTATTSSS 670
            D VQHVATLFFGGPDDREALA  R + +++ I+LTVI+FV    KA + V G A T  +
Sbjct: 613 -DSVQHVATLFFGGPDDREALALCRWLANNTLIHLTVIQFVSEESKAETPV-GNAMTRDN 672

Query: 671 DDGVLMALPSLRTTSSETDNTFLADFYDRHVSTGQVGYVEKQVKNGEETVAELRDIGDMY 730
           ++ V M +     T  ETD +FL +FY+R V+TGQVG++EK V NG  T+  LR+IG+MY
Sbjct: 673 NE-VFMEVLGRNQTEQETDRSFLEEFYNRFVTTGQVGFIEKLVSNGPHTLTILREIGEMY 732

Query: 731 SLFIVGKGGRGHSPLTTGMSDWEECSELGTVGDLLASSDFNISGSVLIIQQHRHQKKDLI 784
           SLF+VGK   G  P+T  M DWEEC ELGTVGD LASS  +++ SVL++Q+ RH     I
Sbjct: 733 SLFVVGK-STGDCPMTVRMKDWEECPELGTVGDFLASS-LDVNASVLVVQRQRHSHDSFI 783

BLAST of CSPI03G47210 vs. TAIR 10
Match: AT2G13620.1 (cation/hydrogen exchanger 15 )

HSP 1 Score: 382.5 bits (981), Expect = 8.2e-106
Identity = 248/766 (32.38%), Postives = 407/766 (53.13%), Query Frame = 0

Query: 22  VQVSFILVLSHFFHLVLKAFGQPGPIAQILAGMVLGPTGLSNIKAIRDVFFQASAADYYE 81
           +Q++ ++V++ FF  +LK F QP  I++IL G+VLGP+ L          F   +    E
Sbjct: 42  LQLTLVVVVTRFFVFILKPFRQPRVISEILGGIVLGPSVLGRSTKFAHTIFPQRSVMVLE 101

Query: 82  IFGFLSRIIFMFLIGLETDFPYILRNLRVAGIVACGGAAVGSVFGIAVSFFLYQQFEEKS 141
               +  + F+FL+G+E D   + +  + A  +A GG  +  + G A SF +++  E+  
Sbjct: 102 TMANVGLLYFLFLVGVEMDIMVVRKTGKRALTIAIGGMVLPFLIGAAFSFSMHRS-EDHL 161

Query: 142 SRFGFFFIVMLILAYTASPIVIRLAAELKFATSDVGKLAISSALINEMACLAVFNAILAL 201
            +  +   + + L+ TA P++ R+ AELK   +++G++++S+AL+N+M    +    +AL
Sbjct: 162 GQGTYILFLGVALSVTAFPVLARILAELKLINTEIGRISMSAALVNDMFAWILLALAIAL 221

Query: 202 -----RSFQEFGKGIFCAVFIAGVVILNKYLASWFNKRNRNQKYLKNMEVFFLLSLVIAA 261
                 SF      I  AVFIA  V + +   +W  ++    +      +  +L+ V+ +
Sbjct: 222 AESDKTSFASLWVMISSAVFIAVCVFVVRPGIAWIIRKTPEGENFSEFHICLILTGVMIS 281

Query: 262 SVIIELQAFNSIVSSFIFGVMFPKEGKSARTLMHKLTYSVHNFVLPIYFGYVGFQFDGNN 321
             I +    +S+  +F+FG++ P  G    TL+ KL   V   +LP++F   G + +   
Sbjct: 282 GFITDAIGTHSVFGAFVFGLVIP-NGPLGLTLIEKLEDFVSGLLLPLFFAISGLKTNIAA 341

Query: 322 LWKMSNVIIVGIMVLLSIGSKMSGTLAACNYLNIPLNEGVFLGFVLNLKGHADLLLIG-G 381
           +   +  + + +++ L+   K+ GT+    +  +P+ EG+ LG +LN KG  +++++  G
Sbjct: 342 IQGPATWLTLFLVIFLACAGKVIGTVIVAFFHGMPVREGITLGLLLNTKGLVEMIVLNVG 401

Query: 382 ASKSILTWSNPRAYNLLLISIVINTIISGPIVALLMRREHKLFSHAHTSLEYTDPTHELR 441
             + +L   +     ++L+++V+  +I+ PIV +L +   K  S+   +++ T P  ELR
Sbjct: 402 KDQKVL--DDETFATMVLVALVMTGVIT-PIVTILYKPVKKSVSYKRRTIQQTKPDSELR 461

Query: 442 ALACAYGPRHLAGIFPLLSSLSGGHTSQLSPFLLHLIELLHKRRTNVSYHE--------L 501
            L C + PR++  I  LL +      S +  ++LHL+EL  +    +  H         L
Sbjct: 462 VLVCVHTPRNVPTIINLLEASHPTKRSPICIYVLHLVELTGRASAMLIVHNTRKSGRPAL 521

Query: 502 EQDELSDDEGYGGNDVLEIHCAIDAFISDTKIFMSLSKAISAFPTLYEDVCNAAEDLRVS 561
            + +   D      +  E H A  A    T        AIS + T++EDVC+ AED RVS
Sbjct: 522 NRTQAQSDHIINAFENYEQHAAFVAVQPLT--------AISPYSTMHEDVCSLAEDKRVS 581

Query: 562 IVILPFHKHQRIDGKMESGKEGIRTTNQKILRHAPCSVGILVDRVQTGFLSFSHLLVSDH 621
            +I+PFHK Q +DG MES     R  NQ +L ++PCSVGILVDR   G    +   VS  
Sbjct: 582 FIIIPFHKQQTVDGGMESTNPAYRLVNQNLLENSPCSVGILVDRGLNGATRLNSNTVS-- 641

Query: 622 VQHVATLFFGGPDDREALAWSRRMISHSRINLTVIRFVPKATSDVEGTATTSSSDDGVLM 681
              VA LFFGGPDDREALA++ RM  H  I LTV+RF+     D   TA+T +++D  L 
Sbjct: 642 -LQVAVLFFGGPDDREALAYAWRMAQHPGITLTVLRFI--HDEDEADTASTRATNDSDLK 701

Query: 682 ALPSLRTTSSETDNTFLADFYDRHVSTGQVGYVEKQVKNGEETVAELRDIGDMYSLFIVG 741
                     + D+ ++  F   +     + Y+EK V NGEETVA +R +   + LFIVG
Sbjct: 702 IPKMDHRKQRQLDDDYINLFRAENAEYESIVYIEKLVSNGEETVAAVRSMDSSHDLFIVG 761

Query: 742 KGGRGHSPLTTGMSDWEECSELGTVGDLLASSDFNISGSVLIIQQH 774
           +G    SPLT G++DW EC ELG +GDLLASSDF  + SVL++QQ+
Sbjct: 762 RGEGMSSPLTAGLTDWSECPELGAIGDLLASSDFAATVSVLVVQQY 789

BLAST of CSPI03G47210 vs. TAIR 10
Match: AT3G17630.1 (cation/H+ exchanger 19 )

HSP 1 Score: 328.6 bits (841), Expect = 1.4e-89
Identity = 224/763 (29.36%), Postives = 390/763 (51.11%), Query Frame = 0

Query: 22  VQVSFILVLSHFFHLVLKAFGQPGPIAQILAGMVLGPTGLSNIKAIRDVFFQASAADYYE 81
           +Q+  ++V +      LK   QP  IA+I+ G++LGP+ L   KA  D  F   +    +
Sbjct: 37  LQIVLVVVFTRLLAYFLKPLKQPRVIAEIIGGILLGPSALGRSKAYLDTIFPKKSLTVLD 96

Query: 82  IFGFLSRIIFMFLIGLETDFPYILRNLRVAGIVACGGAAVGSVFGIAVSFFLYQQFEEKS 141
               +  + F+FL+GLE DF  I +  + + ++A  G ++  + G+  SF L     +  
Sbjct: 97  TLANIGLLFFLFLVGLELDFAAIKKTGKKSLLIAIAGISLPFIVGVGTSFVLSATISKGV 156

Query: 142 SRFGFFFIVMLILAYTASPIVIRLAAELKFATSDVGKLAISSALINEMACLAVFNAILAL 201
            +  F   + + L+ TA P++ R+ AELK  T+D+G++A+S+A +N++A   +    +AL
Sbjct: 157 DQLPFIVFMGVALSITAFPVLARILAELKLLTTDIGRMAMSAAGVNDVAAWILLALAIAL 216

Query: 202 RSFQEFGKG---------IFCAV-FIAGVVILNKYLASWFNKRNRNQKYLKNMEVFFLLS 261
                 G G         + C   F+   V+  K L ++  +R    + +K + V   L+
Sbjct: 217 S-----GDGTSPLVSVWVLLCGTGFVIFAVVAIKPLLAYMARRCPEGEPVKELYVCVTLT 276

Query: 262 LVIAASVIIELQAFNSIVSSFIFGVMFPKEGKSARTLMHKLTYSVHNFVLPIYFGYVGFQ 321
           +V+AAS + +    +++  +F+ G++ PKEG   R L  K+   V   +LP+YF   G +
Sbjct: 277 VVLAASFVTDTIGIHALFGAFVVGIVAPKEGPFCRILTEKIEDLVSGLLLPLYFAASGLK 336

Query: 322 FDGNNLWKMSNVIIVGIMVLLSIGSKMSGTLAACNYLNIPLNEGVFLGFVLNLKGHADLL 381
            D   +    +  ++ +++L +   K+ GT+ +     +P  E V LGF++N KG  +L+
Sbjct: 337 TDVTTIRGAQSWGLLVLVILTTCFGKIVGTVGSSMLCKVPFREAVTLGFLMNTKGLVELI 396

Query: 382 LIG-GASKSILTWSNPRAYNLLLISIVINTIISGPIVALLMRREHKLFSHAHTSLEYTDP 441
           ++  G  + +L   N +A+ +L++  +  T I+ PIV L+ +   K   + H +++  D 
Sbjct: 397 VLNIGKDRKVL---NDQAFAILVLMALFTTFITTPIVMLIYKPARKGAPYKHRTIQRKDH 456

Query: 442 THELRALACAYGPRHLAGIFPLLSSLSG-GHTSQLSPFLLHLIELLHKRRTNVSYHELEQ 501
             ELR LAC +  R++  +  L+ S  G G   +L  + +HL+EL  +       H+   
Sbjct: 457 DSELRILACFHSTRNIPTLINLIESSRGTGKKGRLCVYAMHLMELSERSSAIAMVHKARN 516

Query: 502 DELSD-DEGYGGNDVLEIHCAIDAFISDTKIFMSLSKAISAFPTLYEDVCNAAEDLRVSI 561
           + L   ++     D + I  A +A+     + +    AIS   +++ED+C +A   RV++
Sbjct: 517 NGLPIWNKIERSTDQMVI--AFEAYQHLRAVAVRPMTAISGLSSIHEDICTSAHQKRVAM 576

Query: 562 VILPFHKHQRIDGKMESGKEGIRTTNQKILRHAPCSVGILVDRVQTGFLSFSHLLVSDHV 621
           ++LPFHKHQR+DG MES        NQ++L+ APCSVGILVDR   G    S ++ S+  
Sbjct: 577 ILLPFHKHQRMDGAMESIGHRFHEVNQRVLQRAPCSVGILVDR---GLGGTSQVVASEVA 636

Query: 622 QHVATLFFGGPDDREALAWSRRMISHSRINLTVIRFVPKATSDVEGTATTSSSDDGVLMA 681
             V   FFGG DDREALA+  +M+ H  I LTV +FV        GT       +     
Sbjct: 637 YKVVIPFFGGLDDREALAYGMKMVEHPGITLTVYKFVA-----ARGTLKRFEKSEH---- 696

Query: 682 LPSLRTTSSETDNTFLADFYDRHVSTGQVGYVEKQVKNGEETVAELRDIGDMYSLFIVGK 741
                    ETD  F+ +  +       + Y E+ V++ ++ +A L+ +    +LF+VG+
Sbjct: 697 -DEKEKKEKETDEEFVRELMNDPRGNESLAYEERVVESKDDIIATLKSMSKC-NLFVVGR 756

Query: 742 GGRGHSPLTTGMSDWEECSELGTVGDLLASSDFNISGSVLIIQ 772
                S + +      +C ELG VG LL+SS+F+ + SVL++Q
Sbjct: 757 NAAVASLVKS-----TDCPELGPVGRLLSSSEFSTTASVLVVQ 770

BLAST of CSPI03G47210 vs. TAIR 10
Match: AT5G41610.1 (cation/H+ exchanger 18 )

HSP 1 Score: 310.8 bits (795), Expect = 3.0e-84
Identity = 234/796 (29.40%), Postives = 393/796 (49.37%), Query Frame = 0

Query: 1   MDATHRMVCQDDLFNP----LSSMGVQVSFILVLSHFFHLVLKAFGQPGPIAQILAGMVL 60
           M AT   V Q D  NP    L    +Q+  ++VL+     +L+   QP  IA+++ G++L
Sbjct: 13  MKATSNGVFQGD--NPIDFALPLAILQIVIVIVLTRVLAYLLRPLRQPRVIAEVIGGIML 72

Query: 61  GPTGLSNIKAIRDVFFQASAADYYEIFGFLSRIIFMFLIGLETDFPYILRNLRVAGIVAC 120
           GP+ L   KA  D  F   +    E    L  + F+FL GLE D   + R  + A  +A 
Sbjct: 73  GPSLLGRSKAFLDAVFPKKSLTVLETLANLGLLFFLFLAGLEIDTKALRRTGKKALGIAL 132

Query: 121 GGAAVGSVFGIAVSFFLYQQFEEKSSRFGFFFIVMLILAYTASPIVIRLAAELKFATSDV 180
            G  +    GI  SF L     +  +   F   + + L+ TA P++ R+ AELK  T+++
Sbjct: 133 AGITLPFALGIGSSFVLKATISKGVNSTAFLVFMGVALSITAFPVLARILAELKLLTTEI 192

Query: 181 GKLAISSALINEMACLAVFNAILALR--------SFQEFGKGIFCAVFIAGVVILNKYLA 240
           G+LA+S+A +N++A   +    +AL         S   F  G  CA F+ G   +   + 
Sbjct: 193 GRLAMSAAAVNDVAAWILLALAIALSGSNTSPLVSLWVFLSG--CA-FVIGASFIIPPIF 252

Query: 241 SWFNKRNRNQKYLKNMEVFFLLSLVIAASVIIELQAFNSIVSSFIFGVMFPKEGKSARTL 300
            W ++R    + ++   +   L++V+    I +    +S+  +F+ GV+ PKEG  A  L
Sbjct: 253 RWISRRCHEGEPIEETYICATLAVVLVCGFITDAIGIHSMFGAFVVGVLIPKEGPFAGAL 312

Query: 301 MHKLTYSVHNFVLPIYFGYVGFQFDGNNLWKMSNVIIVGIMVLLSIGS---KMSGTLAAC 360
           + K+   V    LP+YF   G +    N+  +      G++VL++  +   K+ GTL   
Sbjct: 313 VEKVEDLVSGLFLPLYFVASGLK---TNVATIQGAQSWGLLVLVTATACFGKILGTLGVS 372

Query: 361 NYLNIPLNEGVFLGFVLNLKGHADLLLIG-GASKSILTWSNPRAYNLLLISIVINTIISG 420
               IP+ E + LGF++N KG  +L+++  G  + +L   N + + ++++  +  T I+ 
Sbjct: 373 LAFKIPMREAITLGFLMNTKGLVELIVLNIGKDRKVL---NDQTFAIMVLMALFTTFITT 432

Query: 421 PIVALL---MRREHKLFSHAHTSLEYTDPTHELRALACAYGPRHLAGIFPLLSSLSGGHT 480
           P+V  +    RR  K   + H ++E  +   +LR L C +G   +  +  LL +  G   
Sbjct: 433 PVVMAVYKPARRAKKEGEYKHRAVERENTNTQLRILTCFHGAGSIPSMINLLEASRGIEK 492

Query: 481 SQ-LSPFLLHLIELLHKRRTNVSYHELEQDELSDDEGYGGN-DVLEIHCAIDAFISDTKI 540
            + L  + LHL EL  +    +  H++ ++ +      G N D  ++  A  AF   +++
Sbjct: 493 GEGLCVYALHLRELSERSSAILMVHKVRKNGMPFWNRRGVNADADQVVVAFQAFQQLSRV 552

Query: 541 FMSLSKAISAFPTLYEDVCNAAEDLRVSIVILPFHKHQRIDGKMESGKEGIRTTNQKILR 600
            +    AIS+   ++ED+C  A   + +IVILPFHKHQ++DG +E+ +   R  N+++L 
Sbjct: 553 NVRPMTAISSMSDIHEDICTTAVRKKAAIVILPFHKHQQLDGSLETTRGDYRWVNRRVLL 612

Query: 601 HAPCSVGILVDRVQTGFLSFSHLLVSDHVQHVATLFFGGPDDREALAWSRRMISHSRINL 660
            APCSVGI VDR   G    S +   D    V  LFFGGPDDREALA+  RM  H  I L
Sbjct: 613 QAPCSVGIFVDR---GLGGSSQVSAQDVSYSVVVLFFGGPDDREALAYGLRMAEHPGIVL 672

Query: 661 TVIRFV--PKATSDVEGTATTSSSDDGVLMALPSLRTTSSETDNTFLADFYDRHVSTGQV 720
           TV RFV  P+   ++     ++++++       +    + ++D   +++          V
Sbjct: 673 TVFRFVVSPERVGEIVNVEVSNNNNE-------NQSVKNLKSDEEIMSEIRKISSVDESV 732

Query: 721 GYVEKQVKNGEETVAELRDIGDMYSLFIVGKGGRGHSPLTTGMSDWEECSELGTVGDLLA 774
            +VEKQ++N    V    +     +LF+VG+   G   +   + +  EC ELG VG LL 
Sbjct: 733 KFVEKQIENAAVDVRSAIEEVRRSNLFLVGRMPGGE--IALAIRENSECPELGPVGSLLI 785

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SA375.4e-17944.32Cation/H(+) antiporter 1 OS=Arabidopsis thaliana OX=3702 GN=CHX1 PE=2 SV=1[more]
Q9SAK83.4e-17344.12Cation/H(+) antiporter 2 OS=Arabidopsis thaliana OX=3702 GN=CHX2 PE=2 SV=1[more]
Q9SIT51.2e-10432.38Cation/H(+) antiporter 15 OS=Arabidopsis thaliana OX=3702 GN=CHX15 PE=2 SV=1[more]
Q9LUN42.0e-8829.36Cation/H(+) antiporter 19 OS=Arabidopsis thaliana OX=3702 GN=CHX19 PE=2 SV=1[more]
Q9FFR94.3e-8329.40Cation/H(+) antiporter 18 OS=Arabidopsis thaliana OX=3702 GN=CHX18 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LHF40.0e+0099.87Na_H_Exchanger domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G910... [more]
A0A1S3CRQ90.0e+0095.91cation/H(+) antiporter 1-like OS=Cucumis melo OX=3656 GN=LOC103504000 PE=4 SV=1[more]
A0A6J1EY980.0e+0091.59cation/H(+) antiporter 1-like OS=Cucurbita moschata OX=3662 GN=LOC111439593 PE=4... [more]
A0A6J1IDY50.0e+0091.46cation/H(+) antiporter 1-like OS=Cucurbita maxima OX=3661 GN=LOC111471794 PE=4 S... [more]
A0A5D3E5E60.0e+0090.93Cation/H(+) antiporter 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc... [more]
Match NameE-valueIdentityDescription
XP_031737603.10.0e+0099.87cation/H(+) antiporter 1 [Cucumis sativus][more]
XP_008466643.20.0e+0095.91PREDICTED: cation/H(+) antiporter 1-like [Cucumis melo][more]
XP_022932972.10.0e+0091.59cation/H(+) antiporter 1-like [Cucurbita moschata][more]
KAG6571757.10.0e+0091.59Cation/H(+) antiporter 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG70... [more]
XP_022973229.10.0e+0091.46cation/H(+) antiporter 1-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT1G16380.13.9e-18044.32Cation/hydrogen exchanger family protein [more]
AT1G79400.12.4e-17444.12cation/H+ exchanger 2 [more]
AT2G13620.18.2e-10632.38cation/hydrogen exchanger 15 [more]
AT3G17630.11.4e-8929.36cation/H+ exchanger 19 [more]
AT5G41610.13.0e-8429.40cation/H+ exchanger 18 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 770..783
NoneNo IPR availablePANTHERPTHR32468:SF18CATION/H(+) ANTIPORTER 1coord: 21..773
NoneNo IPR availablePANTHERPTHR32468CATION/H + ANTIPORTERcoord: 21..773
IPR006153Cation/H+ exchangerPFAMPF00999Na_H_Exchangercoord: 27..412
e-value: 4.3E-34
score: 117.9
IPR038770Sodium/solute symporter superfamilyGENE3D1.20.1530.20coord: 19..418
e-value: 4.0E-51
score: 176.0

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI03G47210.1CSPI03G47210.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071897 DNA biosynthetic process
biological_process GO:1902600 proton transmembrane transport
biological_process GO:0006885 regulation of pH
biological_process GO:0006812 cation transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0012505 endomembrane system
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0003887 DNA-directed DNA polymerase activity
molecular_function GO:0015299 solute:proton antiporter activity