CSPI03G47000 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI03G47000
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	beta-amyrin 11-oxidase-like
Location	Chr3: 40073549 .. 40074923 (-)
RNA-Seq Expression	CSPI03G47000
Synteny	CSPI03G47000

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTAAGTGTTTGTGTGATTATTGGAAGTGTTTTAGGGGTTTACTTTTTTGTGAGGAAACTAAATGAAGTATGGTATTTGGTTAAGTTAGGAAGAAGAGTTTACAAATCACTTCCTTCTGGTGATTTGGGATGGCCACTTATTGGCTCTTCATTCTCCTTTTACAAAGCATTTAAGGCTGGTGGTGATCCAGATTCTTTCATTCATCATCTTATTTCAAGGTATATTTTTTCATCTCTATTTCACTTTCTTTTGCCTTTTGATTATTCTTCACCGGTCTGACATTATTATATCAATCTATTCAAGTATATTATTATAACGATACCTTTATAAATGTGATGAAAGTGATAGAGATGCATGTCAAATTTAACTAAGATATTTTGTTGCACCTAATGTTTTTCGTCTTATATCATTTTGCTTAAAAAATGGGTATCGTGGTCTATCCACCTAAGGTTTCAAGCTAGCTTTTAAATAGAGAAACCATGAAAACCATGATAAGAAAACTGTAAAAAACCAATACGATTTTATTTTTAAAGAAGCAAAATCAAAGATTTACGTCCCACCTTATAACTAATGGGTTATTTTTTGCTTTCGAAAACTTCAGTTTATAATTACTCATTCCGTCCCTACCTTTGTTTACTTTATTTTCTAGTTACTAATATTTTAAGAACTCATCTAAACTTTTGAATAAAACAGAGGGTTTTTTTTCCTTAAGAACTCATTCTGACCAATAATTTCTAAGCAGAAATGGAAGAGTTGGAATGTACAAAAGCCACCTCTATGGAAGGCCGACCATCATCGTAACAGATCCAGAAATATGTCGACGGATATACTTAGACGACCAAAAGTTCAAACCAAATTATCCAAAATCAGTGAAATTATTAGAAGTAAATGGTCTTTTCTCAAGGATTGATCACAAAACATTCTATAGAATAATGGCATCCCCCTTAAATGGGTATGAGATATTATCTAAATATGTGAGTTTCATTGAGGAAATAATGAGCAAAGGGTTAGAGGAATGGAGCACAATGAAAGAACCCATTGAATTGTTGACCGAAATTGGAAGTCTGCTTTTTAAAGTCATTATTCGTATCTTCTTAGGCAATGAAATTCCTACCCCTACATTGAATAAGCTTGAAGCATTATACAAACACTTGGGTCCAGTAATTTTGTCCATTTTACCTTATGATGTCCCTGGCCTAACCTACCATCATGCACTCAAGGTATACAATTTAGTTCATTTGTATTGATCTTTTAACGTTTTTCTCTCTCGTCATTTTTGGATTTGAGAATATAGAATAGAAGCGTGTGAATAGTCATTGCAGAAGTTCGATCTAGCATCTAGGGCACTCCAGTTGATGATTTCAATGTGTAA

mRNA sequence

ATGTTAAGTGTTTGTGTGATTATTGGAAGTGTTTTAGGGGTTTACTTTTTTGTGAGGAAACTAAATGAAGTATGGTATTTGGTTAAGTTAGGAAGAAGAGTTTACAAATCACTTCCTTCTGGTGATTTGGGATGGCCACTTATTGGCTCTTCATTCTCCTTTTACAAAGCATTTAAGGCTGGTGGTGATCCAGATTCTTTCATTCATCATCTTATTTCAAGAAATGGAAGAGTTGGAATGTACAAAAGCCACCTCTATGGAAGGCCGACCATCATCGTAACAGATCCAGAAATATGTCGACGGATATACTTAGACGACCAAAAGTTCAAACCAAATTATCCAAAATCAGTGAAATTATTAGAAGTAAATGGTCTTTTCTCAAGGATTGATCACAAAACATTCTATAGAATAATGGCATCCCCCTTAAATGGGTATGAGATATTATCTAAATATGTGAGTTTCATTGAGGAAATAATGAGCAAAGGGTTAGAGGAATGGAGCACAATGAAAGAACCCATTGAATTGTTGACCGAAATTGGAAGTCTGCTTTTTAAAGTCATTATTCGTATCTTCTTAGGCAATGAAATTCCTACCCCTACATTGAATAAGCTTGAAGCATTATACAAACACTTGGGTCCAGTAATTTTGTCCATTTTACCTTATGATGTCCCTGGCCTAACCTACCATCATGCACTCAAGGGCACTCCAGTTGATGATTTCAATGTGTAA

Coding sequence (CDS)

ATGTTAAGTGTTTGTGTGATTATTGGAAGTGTTTTAGGGGTTTACTTTTTTGTGAGGAAACTAAATGAAGTATGGTATTTGGTTAAGTTAGGAAGAAGAGTTTACAAATCACTTCCTTCTGGTGATTTGGGATGGCCACTTATTGGCTCTTCATTCTCCTTTTACAAAGCATTTAAGGCTGGTGGTGATCCAGATTCTTTCATTCATCATCTTATTTCAAGAAATGGAAGAGTTGGAATGTACAAAAGCCACCTCTATGGAAGGCCGACCATCATCGTAACAGATCCAGAAATATGTCGACGGATATACTTAGACGACCAAAAGTTCAAACCAAATTATCCAAAATCAGTGAAATTATTAGAAGTAAATGGTCTTTTCTCAAGGATTGATCACAAAACATTCTATAGAATAATGGCATCCCCCTTAAATGGGTATGAGATATTATCTAAATATGTGAGTTTCATTGAGGAAATAATGAGCAAAGGGTTAGAGGAATGGAGCACAATGAAAGAACCCATTGAATTGTTGACCGAAATTGGAAGTCTGCTTTTTAAAGTCATTATTCGTATCTTCTTAGGCAATGAAATTCCTACCCCTACATTGAATAAGCTTGAAGCATTATACAAACACTTGGGTCCAGTAATTTTGTCCATTTTACCTTATGATGTCCCTGGCCTAACCTACCATCATGCACTCAAGGGCACTCCAGTTGATGATTTCAATGTGTAA

Protein sequence

MLSVCVIIGSVLGVYFFVRKLNEVWYLVKLGRRVYKSLPSGDLGWPLIGSSFSFYKAFKAGGDPDSFIHHLISRNGRVGMYKSHLYGRPTIIVTDPEICRRIYLDDQKFKPNYPKSVKLLEVNGLFSRIDHKTFYRIMASPLNGYEILSKYVSFIEEIMSKGLEEWSTMKEPIELLTEIGSLLFKVIIRIFLGNEIPTPTLNKLEALYKHLGPVILSILPYDVPGLTYHHALKGTPVDDFNV*

Homology

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BLAST of CSPI03G47000 vs. ExPASy Swiss-Prot
Match: B5BSX1 (Beta-amyrin 11-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP88D6 PE=1 SV=1)

HSP 1 Score: 184.1 bits (466), Expect = 1.9e-45
Identity = 104/235 (44.26%), Postives = 148/235 (62.98%), Query Frame = 0


Query: 2   LSVCVIIGSVLGVYFFVRKLNEVWYLVKLGRRVYKSLPSGDLGWPLIGSSFSFYKAFKAG 61
           L VC I GS      FVR LN  +Y VKL RR    LP GD+GWPLIG   SF K F + 
Sbjct: 13  LLVCYIFGSK-----FVRNLNGWYYDVKL-RRKEHPLPPGDMGWPLIGDLLSFIKDF-SS 72

Query: 62  GDPDSFIHHLISRNGRVGMYKSHLYGRPTIIVTDPEICRRIYLDDQKFKPNYPKSVK-LL 121
           G PDSFI++L+ + GR G+YK+HL+G P+IIV +P++CRR+  DD  FK  YPKS+K L 
Sbjct: 73  GHPDSFINNLVLKYGRSGIYKTHLFGNPSIIVCEPQMCRRVLTDDVNFKLGYPKSIKELA 132

Query: 122 EVNGLF--SRIDHKTFYRIMASPLNGYEILSKYVSFIEEIMSKGLEEWSTMKEPIELLTE 181
               +   S  +H+ F R++ SP+ G++ L+ Y+  +EEI+   LEE S+MK P+ELL E
Sbjct: 133 RCRPMIDVSNAEHRLFRRLITSPIVGHKALAMYLERLEEIVINSLEELSSMKHPVELLKE 192

Query: 182 IGSLLFKVIIRIFLGNEIPTPTLNKLEALYKHLGPVILSILPYDVPGLTYHHALK 234
           +  + FK I+ +F+G+      + K+ + +  L   + SI P +VPG T+H AL+
Sbjct: 193 MKKVSFKAIVHVFMGSS-NQDIIKKIGSSFTDLYNGMFSI-PINVPGFTFHKALE 238

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BLAST of CSPI03G47000 vs. ExPASy Swiss-Prot
Match: Q9C5Y2 (Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2)

HSP 1 Score: 169.1 bits (427), Expect = 6.2e-41
Identity = 101/232 (43.53%), Postives = 142/232 (61.21%), Query Frame = 0


Query: 6   VIIGSVLGVYFFVRKLNEVW-YLVKLGRRVYKSLPSGDLGWPLIGSSFSFYKAFKAGGDP 65
           +I+G  L V  +V K   VW Y+ KLG + +  LP GDLGWP+IG+ +SF +AFK   DP
Sbjct: 14  IILG--LFVLKWVLKRVNVWIYVSKLGEKKH-YLPPGDLGWPVIGNMWSFLRAFKT-SDP 73

Query: 66  DSFIHHLISRNGRVGMYKSHLYGRPTIIVTDPEICRRIYLDDQKFKPNYPKSVKLLEVNG 125
           +SFI   I+R GR G+YK+H++G P ++VT PE CRR+  DD  F   +PKS   L    
Sbjct: 74  ESFIQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRK 133

Query: 126 LFSRI---DHKTFYRIMASPLNGYEILSKYVSFIEEIMSKGLEEWSTMKEPIELLTEIGS 185
            F  I   +HK   R+ ++P+NG E LS Y+ FIEE ++  LE+WS M E IE L+ +  
Sbjct: 134 SFVGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGE-IEFLSHLRK 193

Query: 186 LLFKVIIRIFLGNEIPTPTLNKLEALYKHLGPVILSILPYDVPGLTYHHALK 234
           L FKVI+ IFL +E     ++ LE  Y +L   + + +  ++PG  YH ALK
Sbjct: 194 LTFKVIMYIFLSSE-SEHVMDSLEREYTNLNYGVRA-MGINLPGFAYHRALK 238

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BLAST of CSPI03G47000 vs. ExPASy Swiss-Prot
Match: O23051 (Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=KAO1 PE=1 SV=1)

HSP 1 Score: 163.3 bits (412), Expect = 3.4e-39
Identity = 94/224 (41.96%), Postives = 131/224 (58.48%), Query Frame = 0


Query: 13  GVYFFVRKLNEVWYLVKLGRRVYKSLPSGDLGWPLIGSSFSFYKAFKAGGDPDSFIHHLI 72
           G+ + VRK+N   Y   LG   +  LP GDLGWP IG+  SF +AFK   DPDSF   LI
Sbjct: 21  GLNWLVRKVNVWLYESSLGENRH-YLPPGDLGWPFIGNMLSFLRAFKT-SDPDSFTRTLI 80

Query: 73  SRNGRVGMYKSHLYGRPTIIVTDPEICRRIYLDDQKFKPNYPKSVKLLEVNGLFSRI--- 132
            R G  G+YK+H++G P+IIVT  + CRR+  DD  FKP +P S   L     F  I   
Sbjct: 81  KRYGPKGIYKAHMFGNPSIIVTTSDTCRRVLTDDDAFKPGWPTSTMELIGRKSFVGISFE 140

Query: 133 DHKTFYRIMASPLNGYEILSKYVSFIEEIMSKGLEEWSTMKEPIELLTEIGSLLFKVIIR 192
           +HK   R+ A+P+NG+E LS Y+ +IEE +   L++W+ M E  E LT +  L F++I+ 
Sbjct: 141 EHKRLRRLTAAPVNGHEALSTYIPYIEENVITVLDKWTKMGE-FEFLTHLRKLTFRIIMY 200

Query: 193 IFLGNEIPTPTLNKLEALYKHLGPVILSILPYDVPGLTYHHALK 234
           IFL +E     ++ LE  Y  L   + + +  ++PG  YH ALK
Sbjct: 201 IFLSSESEN-VMDALEREYTALNYGVRA-MAVNIPGFAYHRALK 239

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BLAST of CSPI03G47000 vs. ExPASy Swiss-Prot
Match: Q5VRM7 (Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 SV=1)

HSP 1 Score: 145.6 bits (366), Expect = 7.4e-34
Identity = 80/237 (33.76%), Postives = 138/237 (58.23%), Query Frame = 0


Query: 1   MLSVCVIIGSVLGVYFFVRKLNEVWYLVKLGRRVYKSLPSGDLGWPLIGSSFSFYKAFKA 60
           +L+  V+ G VL V   VR+ ++   +  LG      LP G++GWP++GS ++F +AFK+
Sbjct: 19  VLAAAVVAGVVL-VDAVVRRAHDWVRVAALGAERRSRLPPGEMGWPMVGSMWAFLRAFKS 78

Query: 61  GGDPDSFIHHLISRNGRVGMYKSHLYGRPTIIVTDPEICRRIYLDDQKFKPNYPKSVKLL 120
            G+PD+FI   I R GR G+Y++ ++  PTI+   PE C+++ +DD+ F   +PK+   L
Sbjct: 79  -GNPDAFIASFIRRFGRTGVYRTFMFSSPTILAVTPEACKQVLMDDEGFVTGWPKATVTL 138

Query: 121 EVNGLF---SRIDHKTFYRIMASPLNGYEILSKYVSFIEEIMSKGLEEWSTMKE-PIELL 180
                F   S  DH+   ++ A+P+NG++ L+ Y+SFI++ +   L  WS+ +   +E L
Sbjct: 139 IGPKSFVNMSYDDHRRIRKLTAAPINGFDALTTYLSFIDQTVVASLRRWSSPESGQVEFL 198

Query: 181 TEIGSLLFKVIIRIFLGNEIPTPTLNKLEALYKHLGPVILSILPYDVPGLTYHHALK 234
           TE+  + FK+I++IF+       T+  LE  Y  L    +  +  ++PG  Y+ AL+
Sbjct: 199 TELRRMTFKIIVQIFMSG-ADDATMEALERSYTDLN-YGMRAMAINLPGFAYYRALR 251

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BLAST of CSPI03G47000 vs. ExPASy Swiss-Prot
Match: Q9AXH9 (Ent-kaurenoic acid oxidase 1 OS=Hordeum vulgare OX=4513 GN=KAO1 PE=1 SV=1)

HSP 1 Score: 140.6 bits (353), Expect = 2.4e-32
Identity = 74/221 (33.48%), Postives = 122/221 (55.20%), Query Frame = 0


Query: 18  VRKLNEVWYLVKLGRRVYKSLPSGDLGWPLIGSSFSFYKAFKAGGDPDSFIHHLISRNGR 77
           VR  +  ++   LG      LP GD+GWPL+G  ++F +AFK+ G PDSFI     R GR
Sbjct: 25  VRAAHAWYWTASLGAGRRGRLPPGDMGWPLVGGMWAFLRAFKS-GRPDSFIDSFARRFGR 84

Query: 78  VGMYKSHLYGRPTIIVTDPEICRRIYLDDQKFKPNYPKSVKLLEVNGLFSRI---DHKTF 137
            G+Y++ ++  PTI+ T PE C+++ +DD  F   +PK+   L     F  +   +H+  
Sbjct: 85  AGLYRAFMFSSPTIMATTPEACKQVLMDDDAFVTGWPKATVALIGPKSFVNMGYDEHRRL 144

Query: 138 YRIMASPLNGYEILSKYVSFIEEIMSKGLEEWSTM--KEPIELLTEIGSLLFKVIIRIFL 197
            ++ A+P+NG++ L+ Y+ FI++ +   L  WS        E LTE+  + F++I++IF+
Sbjct: 145 RKLTAAPINGFDALTSYLGFIDDTVVTTLRGWSERGGDGHFEFLTELRRMTFRIIVQIFM 204

Query: 198 GNEIPTPTLNKLEALYKHLGPVILSILPYDVPGLTYHHALK 234
           G      T  +LE  Y  L    +  +  D+PG  YH A++
Sbjct: 205 GG-ADERTAAELERTYTELN-YGMRAMAIDLPGFAYHKAIR 242

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BLAST of CSPI03G47000 vs. ExPASy TrEMBL
Match: A0A0A0LI74 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G904060 PE=4 SV=1)

HSP 1 Score: 495.0 bits (1273), Expect = 1.8e-136
Identity = 241/242 (99.59%), Postives = 242/242 (100.00%), Query Frame = 0


Query: 1   MLSVCVIIGSVLGVYFFVRKLNEVWYLVKLGRRVYKSLPSGDLGWPLIGSSFSFYKAFKA 60
           MLSVCVIIGSVLGVYFFVRKLNEVWYLVKLGRRVYKSLPSGDLGWPLIGSSFSFYKAFKA
Sbjct: 1   MLSVCVIIGSVLGVYFFVRKLNEVWYLVKLGRRVYKSLPSGDLGWPLIGSSFSFYKAFKA 60

Query: 61  GGDPDSFIHHLISRNGRVGMYKSHLYGRPTIIVTDPEICRRIYLDDQKFKPNYPKSVKLL 120
           GGDPDSFIHHLISRNGRVGMYKSHLYGRPTIIVTDPEICRRIYLDDQKFKPNYPKSVKLL
Sbjct: 61  GGDPDSFIHHLISRNGRVGMYKSHLYGRPTIIVTDPEICRRIYLDDQKFKPNYPKSVKLL 120

Query: 121 EVNGLFSRIDHKTFYRIMASPLNGYEILSKYVSFIEEIMSKGLEEWSTMKEPIELLTEIG 180
           EVNGLFSRIDHKTFYRIMASPLNGYEILSKYVSFIEEIMSKGLEEWSTMKEPIELL+EIG
Sbjct: 121 EVNGLFSRIDHKTFYRIMASPLNGYEILSKYVSFIEEIMSKGLEEWSTMKEPIELLSEIG 180

Query: 181 SLLFKVIIRIFLGNEIPTPTLNKLEALYKHLGPVILSILPYDVPGLTYHHALKGTPVDDF 240
           SLLFKVIIRIFLGNEIPTPTLNKLEALYKHLGPVILSILPYDVPGLTYHHALKGTPVDDF
Sbjct: 181 SLLFKVIIRIFLGNEIPTPTLNKLEALYKHLGPVILSILPYDVPGLTYHHALKGTPVDDF 240

Query: 241 NV 243
           NV
Sbjct: 241 NV 242

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BLAST of CSPI03G47000 vs. ExPASy TrEMBL
Match: A0A6J1GJM1 (beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111454910 PE=3 SV=1)

HSP 1 Score: 390.2 bits (1001), Expect = 6.3e-105
Identity = 182/233 (78.11%), Postives = 206/233 (88.41%), Query Frame = 0


Query: 1   MLSVCVIIGSVLGVYFFVRKLNEVWYLVKLGRRVYKSLPSGDLGWPLIGSSFSFYKAFKA 60
           M++VC IIG+VLGVY FVRKLNE+WYLVKLGR+ YKSLP GD+GWPL+GSSFSFY AF  
Sbjct: 3   MINVCTIIGAVLGVYMFVRKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFYNAFTV 62

Query: 61  GGDPDSFIHHLISRNGRVGMYKSHLYGRPTIIVTDPEICRRIYLDDQKFKPNYPKSVKLL 120
           GGDPDSFI+HL+SRNGRVGMYKSHLYGRPTIIVTDPEICRRIYLDD+ FKPNYPKSVK+L
Sbjct: 63  GGDPDSFIYHLLSRNGRVGMYKSHLYGRPTIIVTDPEICRRIYLDDEIFKPNYPKSVKIL 122

Query: 121 EVNGLFSRIDHKTFYRIMASPLNGYEILSKYVSFIEEIMSKGLEEWSTMKEPIELLTEIG 180
           EVNG F+R DHK+ YRIMA+P+NGYE+LS YV FI+++M+KGLEEWS+MKEPIELL EIG
Sbjct: 123 EVNGFFTRADHKSAYRIMAAPMNGYEVLSHYVDFIDQVMAKGLEEWSSMKEPIELLGEIG 182

Query: 181 SLLFKVIIRIFLGNEIPTPTLNKLEALYKHLGPVILSILPYDVPGLTYHHALK 234
           SL FKVI RIFLG +I    + +LE LYK LGP ILSILPYDVPGL YH ALK
Sbjct: 183 SLFFKVISRIFLGTDIGASRMAELETLYKDLGPAILSILPYDVPGLVYHRALK 235

[Close]

BLAST of CSPI03G47000 vs. ExPASy TrEMBL
Match: A0A6J1GLR1 (beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111455409 PE=3 SV=1)

HSP 1 Score: 386.0 bits (990), Expect = 1.2e-103
Identity = 180/232 (77.59%), Postives = 205/232 (88.36%), Query Frame = 0


Query: 2   LSVCVIIGSVLGVYFFVRKLNEVWYLVKLGRRVYKSLPSGDLGWPLIGSSFSFYKAFKAG 61
           ++VCVIIG VLGVY FVRKLNE+WYLVKLGR+ YKSLP GD+GWPL+GSSFSFY AF  G
Sbjct: 4   INVCVIIGFVLGVYMFVRKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFYNAFTVG 63

Query: 62  GDPDSFIHHLISRNGRVGMYKSHLYGRPTIIVTDPEICRRIYLDDQKFKPNYPKSVKLLE 121
           GDPDSFI+HL+SRNGRVGMYKSH YGRPTIIVTDPEICRRIYLDD+ FKPNYPKSVK+LE
Sbjct: 64  GDPDSFIYHLLSRNGRVGMYKSHFYGRPTIIVTDPEICRRIYLDDEIFKPNYPKSVKILE 123

Query: 122 VNGLFSRIDHKTFYRIMASPLNGYEILSKYVSFIEEIMSKGLEEWSTMKEPIELLTEIGS 181
           VNG F+R DHK+ YRIMA+P+NGYE+LS YV FI+++M+KGLEEWS+MKEPIELL EIGS
Sbjct: 124 VNGFFTRADHKSAYRIMAAPMNGYEVLSHYVDFIDQVMAKGLEEWSSMKEPIELLGEIGS 183

Query: 182 LLFKVIIRIFLGNEIPTPTLNKLEALYKHLGPVILSILPYDVPGLTYHHALK 234
           L FKVI RIFLG +I    + +LE LYK LGP ILSILPYD+PGLT+H ALK
Sbjct: 184 LFFKVISRIFLGTDIGASRMAELETLYKDLGPAILSILPYDLPGLTFHGALK 235

[Close]

BLAST of CSPI03G47000 vs. ExPASy TrEMBL
Match: A0A6J1I8I5 (beta-amyrin 11-oxidase-like OS=Cucurbita maxima OX=3661 GN=LOC111470631 PE=3 SV=1)

HSP 1 Score: 385.6 bits (989), Expect = 1.6e-103
Identity = 181/232 (78.02%), Postives = 205/232 (88.36%), Query Frame = 0


Query: 2   LSVCVIIGSVLGVYFFVRKLNEVWYLVKLGRRVYKSLPSGDLGWPLIGSSFSFYKAFKAG 61
           ++VCVIIG VLGVY FVRKLNE+WYLVKLGR+ YKSLP GD+GWPL+GSSFSFYKAF  G
Sbjct: 4   INVCVIIGFVLGVYMFVRKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFYKAFTVG 63

Query: 62  GDPDSFIHHLISRNGRVGMYKSHLYGRPTIIVTDPEICRRIYLDDQKFKPNYPKSVKLLE 121
           GDPDSFI+HL+SRNGRVGMYKSHLYG+PTIIVTDPEICRRIYLDD+ F+PNYPKSVK+LE
Sbjct: 64  GDPDSFIYHLLSRNGRVGMYKSHLYGKPTIIVTDPEICRRIYLDDEIFQPNYPKSVKILE 123

Query: 122 VNGLFSRIDHKTFYRIMASPLNGYEILSKYVSFIEEIMSKGLEEWSTMKEPIELLTEIGS 181
           VNGLFSR+DH   YRIMA+P+NGY +LS YV FI++IM+KGLE+WST KEPIELL EIGS
Sbjct: 124 VNGLFSRMDHNCAYRIMAAPINGYNVLSHYVDFIDQIMAKGLEKWSTKKEPIELLGEIGS 183

Query: 182 LLFKVIIRIFLGNEIPTPTLNKLEALYKHLGPVILSILPYDVPGLTYHHALK 234
           L FKVI RIFLG +I    + +LE LYK LGP ILSILPYDVPGLT+H ALK
Sbjct: 184 LFFKVISRIFLGTDIGASRMAELETLYKDLGPAILSILPYDVPGLTFHRALK 235

[Close]

BLAST of CSPI03G47000 vs. ExPASy TrEMBL
Match: A0A6J1GJP8 (beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111454912 PE=3 SV=1)

HSP 1 Score: 383.3 bits (983), Expect = 7.8e-103
Identity = 179/232 (77.16%), Postives = 204/232 (87.93%), Query Frame = 0


Query: 2   LSVCVIIGSVLGVYFFVRKLNEVWYLVKLGRRVYKSLPSGDLGWPLIGSSFSFYKAFKAG 61
           ++VC+IIG +LGVY FVRKLNE+WYLVKLGR+ YKSLP GD+GWPL+GSSFSFYKAF  G
Sbjct: 4   INVCIIIGFLLGVYMFVRKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFYKAFIVG 63

Query: 62  GDPDSFIHHLISRNGRVGMYKSHLYGRPTIIVTDPEICRRIYLDDQKFKPNYPKSVKLLE 121
           GDPDSFI+HL+SRNGRVGMYKSHLYG PTIIVTDPEICRRIYLDD+ FKPNYPKSVK+LE
Sbjct: 64  GDPDSFIYHLLSRNGRVGMYKSHLYGSPTIIVTDPEICRRIYLDDEIFKPNYPKSVKILE 123

Query: 122 VNGLFSRIDHKTFYRIMASPLNGYEILSKYVSFIEEIMSKGLEEWSTMKEPIELLTEIGS 181
           VNGLFSR+DHK  YRIMA+P+NGY +LS YV FI++IM+KGLEEWST KEPIEL  EIGS
Sbjct: 124 VNGLFSRMDHKCAYRIMAAPINGYNVLSHYVDFIDQIMAKGLEEWSTKKEPIELFGEIGS 183

Query: 182 LLFKVIIRIFLGNEIPTPTLNKLEALYKHLGPVILSILPYDVPGLTYHHALK 234
           L F+VI RIFLG +I    + +LE LYK LGP ILSILPYD+PGLT+H ALK
Sbjct: 184 LFFRVISRIFLGTDIGASRMAELETLYKDLGPAILSILPYDLPGLTFHRALK 235

[Close]

BLAST of CSPI03G47000 vs. NCBI nr
Match: KAE8651451.1 (hypothetical protein Csa_000969 [Cucumis sativus])

HSP 1 Score: 474.6 bits (1220), Expect = 5.3e-130
Identity = 232/233 (99.57%), Postives = 233/233 (100.00%), Query Frame = 0


Query: 1   MLSVCVIIGSVLGVYFFVRKLNEVWYLVKLGRRVYKSLPSGDLGWPLIGSSFSFYKAFKA 60
           MLSVCVIIGSVLGVYFFVRKLNEVWYLVKLGRRVYKSLPSGDLGWPLIGSSFSFYKAFKA
Sbjct: 4   MLSVCVIIGSVLGVYFFVRKLNEVWYLVKLGRRVYKSLPSGDLGWPLIGSSFSFYKAFKA 63

Query: 61  GGDPDSFIHHLISRNGRVGMYKSHLYGRPTIIVTDPEICRRIYLDDQKFKPNYPKSVKLL 120
           GGDPDSFIHHLISRNGRVGMYKSHLYGRPTIIVTDPEICRRIYLDDQKFKPNYPKSVKLL
Sbjct: 64  GGDPDSFIHHLISRNGRVGMYKSHLYGRPTIIVTDPEICRRIYLDDQKFKPNYPKSVKLL 123

Query: 121 EVNGLFSRIDHKTFYRIMASPLNGYEILSKYVSFIEEIMSKGLEEWSTMKEPIELLTEIG 180
           EVNGLFSRIDHKTFYRIMASPLNGYEILSKYVSFIEEIMSKGLEEWSTMKEPIELL+EIG
Sbjct: 124 EVNGLFSRIDHKTFYRIMASPLNGYEILSKYVSFIEEIMSKGLEEWSTMKEPIELLSEIG 183

Query: 181 SLLFKVIIRIFLGNEIPTPTLNKLEALYKHLGPVILSILPYDVPGLTYHHALK 234
           SLLFKVIIRIFLGNEIPTPTLNKLEALYKHLGPVILSILPYDVPGLTYHHALK
Sbjct: 184 SLLFKVIIRIFLGNEIPTPTLNKLEALYKHLGPVILSILPYDVPGLTYHHALK 236

[Close]

BLAST of CSPI03G47000 vs. NCBI nr
Match: XP_011652661.1 (beta-amyrin 11-oxidase [Cucumis sativus])

HSP 1 Score: 474.6 bits (1220), Expect = 5.3e-130
Identity = 232/233 (99.57%), Postives = 233/233 (100.00%), Query Frame = 0


Query: 1   MLSVCVIIGSVLGVYFFVRKLNEVWYLVKLGRRVYKSLPSGDLGWPLIGSSFSFYKAFKA 60
           MLSVCVIIGSVLGVYFFVRKLNEVWYLVKLGRRVYKSLPSGDLGWPLIGSSFSFYKAFKA
Sbjct: 4   MLSVCVIIGSVLGVYFFVRKLNEVWYLVKLGRRVYKSLPSGDLGWPLIGSSFSFYKAFKA 63

Query: 61  GGDPDSFIHHLISRNGRVGMYKSHLYGRPTIIVTDPEICRRIYLDDQKFKPNYPKSVKLL 120
           GGDPDSFIHHLISRNGRVGMYKSHLYGRPTIIVTDPEICRRIYLDDQKFKPNYPKSVKLL
Sbjct: 64  GGDPDSFIHHLISRNGRVGMYKSHLYGRPTIIVTDPEICRRIYLDDQKFKPNYPKSVKLL 123

Query: 121 EVNGLFSRIDHKTFYRIMASPLNGYEILSKYVSFIEEIMSKGLEEWSTMKEPIELLTEIG 180
           EVNGLFSRIDHKTFYRIMASPLNGYEILSKYVSFIEEIMSKGLEEWSTMKEPIELL+EIG
Sbjct: 124 EVNGLFSRIDHKTFYRIMASPLNGYEILSKYVSFIEEIMSKGLEEWSTMKEPIELLSEIG 183

Query: 181 SLLFKVIIRIFLGNEIPTPTLNKLEALYKHLGPVILSILPYDVPGLTYHHALK 234
           SLLFKVIIRIFLGNEIPTPTLNKLEALYKHLGPVILSILPYDVPGLTYHHALK
Sbjct: 184 SLLFKVIIRIFLGNEIPTPTLNKLEALYKHLGPVILSILPYDVPGLTYHHALK 236

[Close]

BLAST of CSPI03G47000 vs. NCBI nr
Match: XP_038877829.1 (beta-amyrin 11-oxidase-like [Benincasa hispida])

HSP 1 Score: 402.9 bits (1034), Expect = 2.0e-108
Identity = 193/233 (82.83%), Postives = 210/233 (90.13%), Query Frame = 0


Query: 1   MLSVCVIIGSVLGVYFFVRKLNEVWYLVKLGRRVYKSLPSGDLGWPLIGSSFSFYKAFKA 60
           ++S  VIIGSVL VY FVRK NE+WYLVKLGR+VYKSLP GD GWP IG S SFYKAFKA
Sbjct: 3   IISAYVIIGSVLWVYLFVRKFNEIWYLVKLGRKVYKSLPPGDFGWPFIGYSLSFYKAFKA 62

Query: 61  GGDPDSFIHHLISRNGRVGMYKSHLYGRPTIIVTDPEICRRIYLDDQKFKPNYPKSVKLL 120
           GGDPDSFI HL+S+NGRVGMYKSHLYGRPTIIVTD EICR IYLDD+KFKPNYPKSVK+L
Sbjct: 63  GGDPDSFISHLLSKNGRVGMYKSHLYGRPTIIVTDLEICRLIYLDDEKFKPNYPKSVKIL 122

Query: 121 EVNGLFSRIDHKTFYRIMASPLNGYEILSKYVSFIEEIMSKGLEEWSTMKEPIELLTEIG 180
           EVNGLFSRIDHKT Y+IMASP+NGYEILS YVSFI++ M+KGLEEWST KEPIELL+EIG
Sbjct: 123 EVNGLFSRIDHKTAYKIMASPINGYEILSNYVSFIDQTMAKGLEEWSTAKEPIELLSEIG 182

Query: 181 SLLFKVIIRIFLGNEIPTPTLNKLEALYKHLGPVILSILPYDVPGLTYHHALK 234
           SLLFKVII IFLGNEI  PT+ KLE LYKHLGP ILSILPYD+PGLT+HHALK
Sbjct: 183 SLLFKVIIHIFLGNEITAPTMAKLEELYKHLGPAILSILPYDLPGLTFHHALK 235

[Close]

BLAST of CSPI03G47000 vs. NCBI nr
Match: XP_023511634.1 (beta-amyrin 11-oxidase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 392.1 bits (1006), Expect = 3.4e-105
Identity = 183/232 (78.88%), Postives = 208/232 (89.66%), Query Frame = 0


Query: 2   LSVCVIIGSVLGVYFFVRKLNEVWYLVKLGRRVYKSLPSGDLGWPLIGSSFSFYKAFKAG 61
           ++VCVIIG VLGVY FVRKLNE+WYLVKLGR+ YKSLP GD+GWPL+GSSFSFYKAF  G
Sbjct: 4   INVCVIIGFVLGVYMFVRKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFYKAFTVG 63

Query: 62  GDPDSFIHHLISRNGRVGMYKSHLYGRPTIIVTDPEICRRIYLDDQKFKPNYPKSVKLLE 121
           GDPDSFI+HL++RNGRVGMYKSHLYGRPTIIVTDPEICRRIYLDD+ FKPNYPKSVK+LE
Sbjct: 64  GDPDSFIYHLLTRNGRVGMYKSHLYGRPTIIVTDPEICRRIYLDDEIFKPNYPKSVKILE 123

Query: 122 VNGLFSRIDHKTFYRIMASPLNGYEILSKYVSFIEEIMSKGLEEWSTMKEPIELLTEIGS 181
           VNGLFSRIDHK+ YRIM +P+NGYE+LS YV FI+++M+KGLEEWS+MKEPIELL EIGS
Sbjct: 124 VNGLFSRIDHKSAYRIMVAPMNGYEVLSHYVDFIDQVMAKGLEEWSSMKEPIELLGEIGS 183

Query: 182 LLFKVIIRIFLGNEIPTPTLNKLEALYKHLGPVILSILPYDVPGLTYHHALK 234
           L FKVI RIFLG +I    + +LE LYK LGP ILSILPYD+PGLT+H ALK
Sbjct: 184 LFFKVISRIFLGTDIGASRMAELETLYKDLGPAILSILPYDLPGLTFHRALK 235

[Close]

BLAST of CSPI03G47000 vs. NCBI nr
Match: KAG6571834.1 (Beta-amyrin 11-oxidase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 392.1 bits (1006), Expect = 3.4e-105
Identity = 183/233 (78.54%), Postives = 207/233 (88.84%), Query Frame = 0


Query: 1   MLSVCVIIGSVLGVYFFVRKLNEVWYLVKLGRRVYKSLPSGDLGWPLIGSSFSFYKAFKA 60
           M++VC IIG+VLGVY FVRKLNE+WYLVKLGR+ YKSLP GD+GWPL+GSSFSFYKAF  
Sbjct: 3   MINVCTIIGAVLGVYMFVRKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFYKAFTV 62

Query: 61  GGDPDSFIHHLISRNGRVGMYKSHLYGRPTIIVTDPEICRRIYLDDQKFKPNYPKSVKLL 120
           GGDPDSFI+HL+SRNGRVGMYKSHLYGRPTIIVTDPEICRRIYLDD+ FKPNYPKSVK+L
Sbjct: 63  GGDPDSFIYHLLSRNGRVGMYKSHLYGRPTIIVTDPEICRRIYLDDEIFKPNYPKSVKIL 122

Query: 121 EVNGLFSRIDHKTFYRIMASPLNGYEILSKYVSFIEEIMSKGLEEWSTMKEPIELLTEIG 180
           EVNG F+R DHK+ YRIMA+P+NGYE+LS YV FI+++M+KGLEEWS+MKEPIELL EIG
Sbjct: 123 EVNGFFTRADHKSAYRIMAAPMNGYEVLSHYVDFIDQVMAKGLEEWSSMKEPIELLGEIG 182

Query: 181 SLLFKVIIRIFLGNEIPTPTLNKLEALYKHLGPVILSILPYDVPGLTYHHALK 234
           SL FKVI RIFLG +I    + +LE LYK LGP ILSILPYDVPGL YH ALK
Sbjct: 183 SLFFKVISRIFLGTDIGASRMAELETLYKDLGPAILSILPYDVPGLVYHRALK 235

[Close]

BLAST of CSPI03G47000 vs. TAIR 10
Match: AT2G32440.1 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 169.1 bits (427), Expect = 4.4e-42
Identity = 101/232 (43.53%), Postives = 142/232 (61.21%), Query Frame = 0


Query: 6   VIIGSVLGVYFFVRKLNEVW-YLVKLGRRVYKSLPSGDLGWPLIGSSFSFYKAFKAGGDP 65
           +I+G  L V  +V K   VW Y+ KLG + +  LP GDLGWP+IG+ +SF +AFK   DP
Sbjct: 14  IILG--LFVLKWVLKRVNVWIYVSKLGEKKH-YLPPGDLGWPVIGNMWSFLRAFKT-SDP 73

Query: 66  DSFIHHLISRNGRVGMYKSHLYGRPTIIVTDPEICRRIYLDDQKFKPNYPKSVKLLEVNG 125
           +SFI   I+R GR G+YK+H++G P ++VT PE CRR+  DD  F   +PKS   L    
Sbjct: 74  ESFIQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRK 133

Query: 126 LFSRI---DHKTFYRIMASPLNGYEILSKYVSFIEEIMSKGLEEWSTMKEPIELLTEIGS 185
            F  I   +HK   R+ ++P+NG E LS Y+ FIEE ++  LE+WS M E IE L+ +  
Sbjct: 134 SFVGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGE-IEFLSHLRK 193

Query: 186 LLFKVIIRIFLGNEIPTPTLNKLEALYKHLGPVILSILPYDVPGLTYHHALK 234
           L FKVI+ IFL +E     ++ LE  Y +L   + + +  ++PG  YH ALK
Sbjct: 194 LTFKVIMYIFLSSE-SEHVMDSLEREYTNLNYGVRA-MGINLPGFAYHRALK 238

[Close]

BLAST of CSPI03G47000 vs. TAIR 10
Match: AT2G32440.2 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 169.1 bits (427), Expect = 4.4e-42
Identity = 101/232 (43.53%), Postives = 142/232 (61.21%), Query Frame = 0


Query: 6   VIIGSVLGVYFFVRKLNEVW-YLVKLGRRVYKSLPSGDLGWPLIGSSFSFYKAFKAGGDP 65
           +I+G  L V  +V K   VW Y+ KLG + +  LP GDLGWP+IG+ +SF +AFK   DP
Sbjct: 14  IILG--LFVLKWVLKRVNVWIYVSKLGEKKH-YLPPGDLGWPVIGNMWSFLRAFKT-SDP 73

Query: 66  DSFIHHLISRNGRVGMYKSHLYGRPTIIVTDPEICRRIYLDDQKFKPNYPKSVKLLEVNG 125
           +SFI   I+R GR G+YK+H++G P ++VT PE CRR+  DD  F   +PKS   L    
Sbjct: 74  ESFIQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRK 133

Query: 126 LFSRI---DHKTFYRIMASPLNGYEILSKYVSFIEEIMSKGLEEWSTMKEPIELLTEIGS 185
            F  I   +HK   R+ ++P+NG E LS Y+ FIEE ++  LE+WS M E IE L+ +  
Sbjct: 134 SFVGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGE-IEFLSHLRK 193

Query: 186 LLFKVIIRIFLGNEIPTPTLNKLEALYKHLGPVILSILPYDVPGLTYHHALK 234
           L FKVI+ IFL +E     ++ LE  Y +L   + + +  ++PG  YH ALK
Sbjct: 194 LTFKVIMYIFLSSE-SEHVMDSLEREYTNLNYGVRA-MGINLPGFAYHRALK 238

[Close]

BLAST of CSPI03G47000 vs. TAIR 10
Match: AT1G05160.1 (cytochrome P450, family 88, subfamily A, polypeptide 3 )

HSP 1 Score: 163.3 bits (412), Expect = 2.4e-40
Identity = 94/224 (41.96%), Postives = 131/224 (58.48%), Query Frame = 0


Query: 13  GVYFFVRKLNEVWYLVKLGRRVYKSLPSGDLGWPLIGSSFSFYKAFKAGGDPDSFIHHLI 72
           G+ + VRK+N   Y   LG   +  LP GDLGWP IG+  SF +AFK   DPDSF   LI
Sbjct: 21  GLNWLVRKVNVWLYESSLGENRH-YLPPGDLGWPFIGNMLSFLRAFKT-SDPDSFTRTLI 80

Query: 73  SRNGRVGMYKSHLYGRPTIIVTDPEICRRIYLDDQKFKPNYPKSVKLLEVNGLFSRI--- 132
            R G  G+YK+H++G P+IIVT  + CRR+  DD  FKP +P S   L     F  I   
Sbjct: 81  KRYGPKGIYKAHMFGNPSIIVTTSDTCRRVLTDDDAFKPGWPTSTMELIGRKSFVGISFE 140

Query: 133 DHKTFYRIMASPLNGYEILSKYVSFIEEIMSKGLEEWSTMKEPIELLTEIGSLLFKVIIR 192
           +HK   R+ A+P+NG+E LS Y+ +IEE +   L++W+ M E  E LT +  L F++I+ 
Sbjct: 141 EHKRLRRLTAAPVNGHEALSTYIPYIEENVITVLDKWTKMGE-FEFLTHLRKLTFRIIMY 200

Query: 193 IFLGNEIPTPTLNKLEALYKHLGPVILSILPYDVPGLTYHHALK 234
           IFL +E     ++ LE  Y  L   + + +  ++PG  YH ALK
Sbjct: 201 IFLSSESEN-VMDALEREYTALNYGVRA-MAVNIPGFAYHRALK 239

[Close]

BLAST of CSPI03G47000 vs. TAIR 10
Match: AT5G36110.1 (cytochrome P450, family 716, subfamily A, polypeptide 1 )

HSP 1 Score: 82.4 bits (202), Expect = 5.4e-16
Identity = 64/207 (30.92%), Postives = 104/207 (50.24%), Query Frame = 0


Query: 35  YKSLPSGDLGWPLIGSSFSFYKAFKAGGDPDSFIHHLISR--NGRVGMYKSHLYGRPTII 94
           Y +LP G+ G PLIG SFSF  A +  G P+ FI   + R  +    ++K+HL+G PT +
Sbjct: 31  YPNLPPGNTGLPLIGESFSFLSAGRQ-GHPEKFITDRVRRFSSSSSCVFKTHLFGSPTAV 90

Query: 95  VTDPEICRRIYLDDQKFKPN-YPKSV-KLLEVNGLFSRIDHKTFYRIMASPLNGYEILSK 154
           VT     + ++ ++ K   + +P SV K+   +   S  +     R++ S     E L +
Sbjct: 91  VTGASGNKFLFTNENKLVVSWWPDSVNKIFPSSMQTSSKEEARKLRMLLSQFMKPEALRR 150

Query: 155 YVSFIEEIMSKGLE-EWSTMKEPIEL-LTEIGSLLFKVIIRIFLGNEIPTPTLNKLEALY 214
           YV  ++EI  +  E EW+   + I   LT+     F +  R FL  E P   + +LE  +
Sbjct: 151 YVGVMDEIAQRHFETEWANQDQVIVFPLTK--KFTFSIACRSFLSMEDPA-RVRQLEEQF 210

Query: 215 KHLGPVILSILPYDVPGLTYHHALKGT 236
             +   I SI P D+PG  ++ A+K +
Sbjct: 211 NTVAVGIFSI-PIDLPGTRFNRAIKAS 232

[Close]

BLAST of CSPI03G47000 vs. TAIR 10
Match: AT3G13730.1 (cytochrome P450, family 90, subfamily D, polypeptide 1 )

HSP 1 Score: 81.3 bits (199), Expect = 1.2e-15
Identity = 58/203 (28.57%), Postives = 103/203 (50.74%), Query Frame = 0


Query: 39  PSGDLGWPLIGSSFSFYKAFKAGGDPDSFIHHLISRNGRVGMYKSHLYGRPTIIVTDPEI 98
           P G LGWP+IG +  F  +      P+SF+       GRV  +KSH++G  TI+ TD E+
Sbjct: 52  PHGSLGWPVIGETIEFVSS-AYSDRPESFMDKRRLMYGRV--FKSHIFGTATIVSTDAEV 111

Query: 99  CRRIYLDDQ-KFKPNYPKSVK-------LLEVNGLFSRIDHKTFYRIMASPLNGYEILSK 158
            R +   D   F P YPK+V+       +L +NG      H+ F+ ++ S L    + ++
Sbjct: 112 NRAVLQSDSTAFVPFYPKTVRELMGKSSILLINGSL----HRRFHGLVGSFLKSPLLKAQ 171

Query: 159 YVSFIEEIMSKGLEEWSTMKEPIELLTEIGSLLFKVIIRIFLGNEIPTPTLNKLEALYKH 218
            V  + + +S+ ++ WS   +P+ L     ++ FKV+ +  +  E     L +L+  +++
Sbjct: 172 IVRDMHKFLSESMDLWSE-DQPVLLQDVSKTVAFKVLAKALISVE-KGEDLEELKREFEN 231

Query: 219 LGPVILSILPYDVPGLTYHHALK 234
               ++S LP + PG   H +L+
Sbjct: 232 FISGLMS-LPINFPGTQLHRSLQ 244

The following BLAST results are available for this feature:

BLAST of CSPI03G47000 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
B5BSX1	1.9e-45	44.26	Beta-amyrin 11-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP88D6 PE=1 SV=1	[more]
Q9C5Y2	6.2e-41	43.53	Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2	[more]
O23051	3.4e-39	41.96	Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=KAO1 PE=1 SV=1	[more]
Q5VRM7	7.4e-34	33.76	Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 ...	[more]
Q9AXH9	2.4e-32	33.48	Ent-kaurenoic acid oxidase 1 OS=Hordeum vulgare OX=4513 GN=KAO1 PE=1 SV=1	[more]

BLAST of CSPI03G47000 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LI74	1.8e-136	99.59	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G904060 PE=4 SV=1	[more]
A0A6J1GJM1	6.3e-105	78.11	beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111454910 PE=3 S...	[more]
A0A6J1GLR1	1.2e-103	77.59	beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111455409 PE=3 S...	[more]
A0A6J1I8I5	1.6e-103	78.02	beta-amyrin 11-oxidase-like OS=Cucurbita maxima OX=3661 GN=LOC111470631 PE=3 SV=...	[more]
A0A6J1GJP8	7.8e-103	77.16	beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111454912 PE=3 S...	[more]

BLAST of CSPI03G47000 vs. NCBI nr


Match Name	E-value	Identity	Description
KAE8651451.1	5.3e-130	99.57	hypothetical protein Csa_000969 [Cucumis sativus]	[more]
XP_011652661.1	5.3e-130	99.57	beta-amyrin 11-oxidase [Cucumis sativus]	[more]
XP_038877829.1	2.0e-108	82.83	beta-amyrin 11-oxidase-like [Benincasa hispida]	[more]
XP_023511634.1	3.4e-105	78.88	beta-amyrin 11-oxidase-like [Cucurbita pepo subsp. pepo]	[more]
KAG6571834.1	3.4e-105	78.54	Beta-amyrin 11-oxidase, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of CSPI03G47000 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G32440.1	4.4e-42	43.53	ent-kaurenoic acid hydroxylase 2	[more]
AT2G32440.2	4.4e-42	43.53	ent-kaurenoic acid hydroxylase 2	[more]
AT1G05160.1	2.4e-40	41.96	cytochrome P450, family 88, subfamily A, polypeptide 3	[more]
AT5G36110.1	5.4e-16	30.92	cytochrome P450, family 716, subfamily A, polypeptide 1	[more]
AT3G13730.1	1.2e-15	28.57	cytochrome P450, family 90, subfamily D, polypeptide 1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 32..236 e-value: 1.3E-18 score: 69.1
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 36..213
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 39..202 e-value: 1.4E-5 score: 24.0
None	No IPR available	PANTHER	PTHR24286	CYTOCHROME P450 26	coord: 10..233
None	No IPR available	PANTHER	PTHR24286:SF289	ENT-KAURENOIC ACID OXIDASE 2	coord: 10..233

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G47000.1	CSPI03G47000.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016132	brassinosteroid biosynthetic process
biological_process	GO:0010268	brassinosteroid homeostasis
biological_process	GO:0016125	sterol metabolic process
cellular_component	GO:0005783	endoplasmic reticulum
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0051777	ent-kaurenoate oxidase activity
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen