CSPI03G41480 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI03G41480
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionCytochrome P450
LocationChr3: 35608147 .. 35611655 (+)
RNA-Seq ExpressionCSPI03G41480
SyntenyCSPI03G41480
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CAATTGCGAAACCTCAAACCAAATCTATACACACAGTACATATCCAACTTCAAAAGATTCATAAAAAAAAAATGGATTCTTTGTGGATATTTGTGCAGCTCATCACAGCTGCAGCTTTCATATCTTCCACAATCATAATCCTCAGAAACTTCATCATCCCCACCACACCATTGTCATCTTCTTCAAACTCCTTCACCTCCTCCAACAATCCTTCACTTCCTTCCGGCTCCCTCGGTCTCCCCTTCGTTGGTGAAACCCTCCACTTCGTCTCTTCTGCTTACTCTCACCGCCCCGACACCTTCATGGACACACGTCGCCGACGGTGAGTTTCTGGGTTAGAGATAAAAAGTTTCATAGAGTCGTCAACCGTCATCCCGTGTGAAATAACTCCATATCTCATGTTTTTTTTCCCTAGATATATATAGTGATTCAAATGACAAATCTCTTGGTCGCTAACATGTTTGTATGTCAGTTGACCTTTGGTCACTATATTTGGTGATTAAGAACATGGAAAAATATTATGAGATCTCTTTTTTTTTTTTTTGCAGGTATGGGAAGGTTTTCAAATCGCATATATTTGGAAGTCCAACAATAGTTTCAACAGATGGTGAAGTGAATAGGTTTATTCTACAAAGTGATTCAAAGTATTTTGTGCCATCTTATCCAAAATCTGTGACAGAGTTGATGGGAAAATCTTCTATTTTGCTTATCAATGGCACTTTACATAGAAGAATTCATGGTCTTATTGGAGCTTTTTTCAAATCATCTCATCTCAAAGCTCAAATAACCTTAGACATGCAAAAATATCTTCACAAATCCATCTCTACTTGGACTAGTACTTGTCAACAGAATCCTATCCACATTCAAGATGAAGCCAAAAATGTTTGTATACCTCATCTCAATCTTCTTGTAATTCAGTCAACTTTCTCGTAATCTATCAACTTAAGTTTTATTCATATATCTTTAGATAACGTTATTTCATTCACAAGAAACGAGTCCAATAATATATATTTGGGCGAAGTACAAAGTATATTAAGAATATCTTAAATAGATGATTAAAATTTTAGGGTTAAGAACTGTTCTCATTTGAAACATGGGTTGGGTCATAAGTTTGCTTAGAATCATATTGTCCAAAGAAGCTCACACATTCAGAGTCATTATTATTAGTCAAATCTTTTTCATTTTTCTCAATTAAGAGAACCAAAAAACAAAAAAAAGTTTTGTTTTTTTTTTAATATAAATTGTATTTGATTAATTTGTTTCAGATTGCCTTTGAAGTACTAGTAAAGACACTGATCAGTTTGGAGAGTGGTGAAGAAATGGAATTTCTGAAGAAACAGTTTAATCAATTCATCGCTGGGATTATGGCTTTCCCAATAAACATCCCAGGAACCACACTTTACAGATCATTACAGGCAAGTTTATAAAAGTTTTTATGTTTTCATATAATTGAATTTAGGGGAAAAATAATTCTAACAGTCCCTAAGTTTTTGGATCTACTTTTGTTTGGTTCCTATATACTTTTTAATAGTGTCAAAATTAATAATTAATCATAAATATTTTTTAAAGTTAACTGGTAGACGCTAAAAACTAAAAGTATTCTTGTAAAAGTTTATATTTAATCAAAATTTGAAATTTATGATCAAGTTTGAAAGAAAATTCAAATTTCAAAAGCAAAGTTGTGAAATTTAAGGACTAAATTAAAATGAACAAATTAAACTCAAAATATTTAATAAGACTAAACATGTAATATTTTAAAACCTAAGCATGAAATGAAAAATTAAGTAATAAACTCAAAACCTAGGATTAAAAGAAGGTATTTTCATTTGAAAATTACAACAAATTAAGAAGATCAGTAAAATGAAGCAAAATTGGTGTGTTTGTAATTTTTTTTTTGTGAAAATGTTTCAGGCAAAAAGAAAAATGGTGAAGTTAGTGGAAGAAATAATAGAAGGAAGAAGAAAGAAGAAGAAGATCAAGAGTGAAAAATGGAGTAACAATCACAATAATAATAAGAATGGAGTTGGAATAATAAATGATGTTGTGGATGTTTTGTTGAATGATGGAAGCCAACAAGTAACAGATGAAGTTATAGCTCATAATATGATTGATATGATGATTCCAGGCCAAGATTCTGTCCCAATCCTTATCACTTTGGCCATTAAATACCTTTCTGATTCTCCCTCTGCCCTTCAACAATTAACGGTACCTTCATCATACGCTCTCTCTCCTTCCTATTTTAATGTCTGTATTTGAACTCACTGCTCAAATCTAAATCTATACATATTCCAATTCAATTCAATCTGTATTTACTTAGGCTTGTTAACACTCTTAACTATTAAGAGAGATTAAGAGAGGGTGTAATAATATGTGTTTTAATAAAAATGCTATATCCTAAAATTGAGAATTTTTTTTCTTGTTGTATGTTTTAAAATAGAAACTGATGTTACATTTAAATTAGTCATTTTGGCTTTTCTTCCTTATGATGTGTGTTTCATTTGACAGTGAAAGTTTCTGTCATTTTGGTGACAACCACATCAACAATACAAAAATATAACCCTCTCTCTCTCCACTCTCTCATAATTAATTATGATCTTACCTTTTTATTTTCCTTTCCTTTCTTTTTCCAATATATATACTCATCTTTTTTCACAAAAAGATTTCTTTTAGTAAACAAAATTAAACTTCATGGAAAATAATTAAAGGATCATGAAAGAAAGATATCATTCATGAACGTATATAATGTGGCACAAACAACAACAACTATTTTATTTATTTCAGCAGTCAAAACCCCAATGAATTTAAAATACTATTTACTATTATTATATTATATTTGTATGTGTATGTGTGTACATATTTCCCTAAACTTAAAAATTTTCAATGGGCAGGAAGAGAACATGGAATTGAAAAGACAGAAAACTCAGTTTGGAGAGCCATTGAAGTGGAGTGATTACTTGTCTTTACCATTTACACAAAATGTAAGACACACATCCACATTACTGGACAATATACCAAATATTTGCTATAATAAAATAAAAATTAAATTATGGTTGTGAGTATATCAATATAATACGTTTTCTTTTTAATTCTGGCGATATTCAGGTGATAACAGAGACGTTAAGGTTAGGAAACATAATAACAGGAGTGATGAGAAAGGCAATGAAAGACATTGAAATAAAAGGATATTTAATACCAAAAGGATGGTGTGTACTTGCATATATTAGGTCAGTTCATGTGGATGAAAACCACTTTGAAAGCCCTTACCATTTCAATCCATGGAGATGGCAAGTAAGTATACACTCATTAATTGCCCTAATTATTTTTGGGTCTTTTTTATTAATTGTTATTTAATAAGGAAAATTTAAATTTTTGTTATCTTTCAGGGTAAAGACTCAAATAATTTGAATTTCAGTCCTTTTGGAGGAGGACAAAGGTTGTGCCCAGGCCTTGAATTAGCCAGATTAGAAGCTTCCATTTTCTTACATCACTTCGTTACTGAATTCAGGTAA

mRNA sequence

CAATTGCGAAACCTCAAACCAAATCTATACACACAGTACATATCCAACTTCAAAAGATTCATAAAAAAAAAATGGATTCTTTGTGGATATTTGTGCAGCTCATCACAGCTGCAGCTTTCATATCTTCCACAATCATAATCCTCAGAAACTTCATCATCCCCACCACACCATTGTCATCTTCTTCAAACTCCTTCACCTCCTCCAACAATCCTTCACTTCCTTCCGGCTCCCTCGGTCTCCCCTTCGTTGGTGAAACCCTCCACTTCGTCTCTTCTGCTTACTCTCACCGCCCCGACACCTTCATGGACACACGTCGCCGACGGTATGGGAAGGTTTTCAAATCGCATATATTTGGAAGTCCAACAATAGTTTCAACAGATGGTGAAGTGAATAGGTTTATTCTACAAAGTGATTCAAAGTATTTTGTGCCATCTTATCCAAAATCTGTGACAGAGTTGATGGGAAAATCTTCTATTTTGCTTATCAATGGCACTTTACATAGAAGAATTCATGGTCTTATTGGAGCTTTTTTCAAATCATCTCATCTCAAAGCTCAAATAACCTTAGACATGCAAAAATATCTTCACAAATCCATCTCTACTTGGACTAGTACTTGTCAACAGAATCCTATCCACATTCAAGATGAAGCCAAAAATGTTTGTATACCTCATCTCAATCTTCTTATTGCCTTTGAAGTACTAGTAAAGACACTGATCAGTTTGGAGAGTGGTGAAGAAATGGAATTTCTGAAGAAACAGTTTAATCAATTCATCGCTGGGATTATGGCTTTCCCAATAAACATCCCAGGAACCACACTTTACAGATCATTACAGGCAAAAAGAAAAATGGTGAAGTTAGTGGAAGAAATAATAGAAGGAAGAAGAAAGAAGAAGAAGATCAAGAGTGAAAAATGGAGTAACAATCACAATAATAATAAGAATGGAGTTGGAATAATAAATGATGTTGTGGATGTTTTGTTGAATGATGGAAGCCAACAAGTAACAGATGAAGTTATAGCTCATAATATGATTGATATGATGATTCCAGGCCAAGATTCTGTCCCAATCCTTATCACTTTGGCCATTAAATACCTTTCTGATTCTCCCTCTGCCCTTCAACAATTAACGGAAGAGAACATGGAATTGAAAAGACAGAAAACTCAGTTTGGAGAGCCATTGAAGTGGAGTGATTACTTGTCTTTACCATTTACACAAAATGTGATAACAGAGACGTTAAGGTTAGGAAACATAATAACAGGAGTGATGAGAAAGGCAATGAAAGACATTGAAATAAAAGGATATTTAATACCAAAAGGATGGTGTGTACTTGCATATATTAGGTCAGTTCATGTGGATGAAAACCACTTTGAAAGCCCTTACCATTTCAATCCATGGAGATGGCAAGGTAAAGACTCAAATAATTTGAATTTCAGTCCTTTTGGAGGAGGACAAAGGTTGTGCCCAGGCCTTGAATTAGCCAGATTAGAAGCTTCCATTTTCTTACATCACTTCGTTACTGAATTCAGGTAA

Coding sequence (CDS)

ATGGATTCTTTGTGGATATTTGTGCAGCTCATCACAGCTGCAGCTTTCATATCTTCCACAATCATAATCCTCAGAAACTTCATCATCCCCACCACACCATTGTCATCTTCTTCAAACTCCTTCACCTCCTCCAACAATCCTTCACTTCCTTCCGGCTCCCTCGGTCTCCCCTTCGTTGGTGAAACCCTCCACTTCGTCTCTTCTGCTTACTCTCACCGCCCCGACACCTTCATGGACACACGTCGCCGACGGTATGGGAAGGTTTTCAAATCGCATATATTTGGAAGTCCAACAATAGTTTCAACAGATGGTGAAGTGAATAGGTTTATTCTACAAAGTGATTCAAAGTATTTTGTGCCATCTTATCCAAAATCTGTGACAGAGTTGATGGGAAAATCTTCTATTTTGCTTATCAATGGCACTTTACATAGAAGAATTCATGGTCTTATTGGAGCTTTTTTCAAATCATCTCATCTCAAAGCTCAAATAACCTTAGACATGCAAAAATATCTTCACAAATCCATCTCTACTTGGACTAGTACTTGTCAACAGAATCCTATCCACATTCAAGATGAAGCCAAAAATGTTTGTATACCTCATCTCAATCTTCTTATTGCCTTTGAAGTACTAGTAAAGACACTGATCAGTTTGGAGAGTGGTGAAGAAATGGAATTTCTGAAGAAACAGTTTAATCAATTCATCGCTGGGATTATGGCTTTCCCAATAAACATCCCAGGAACCACACTTTACAGATCATTACAGGCAAAAAGAAAAATGGTGAAGTTAGTGGAAGAAATAATAGAAGGAAGAAGAAAGAAGAAGAAGATCAAGAGTGAAAAATGGAGTAACAATCACAATAATAATAAGAATGGAGTTGGAATAATAAATGATGTTGTGGATGTTTTGTTGAATGATGGAAGCCAACAAGTAACAGATGAAGTTATAGCTCATAATATGATTGATATGATGATTCCAGGCCAAGATTCTGTCCCAATCCTTATCACTTTGGCCATTAAATACCTTTCTGATTCTCCCTCTGCCCTTCAACAATTAACGGAAGAGAACATGGAATTGAAAAGACAGAAAACTCAGTTTGGAGAGCCATTGAAGTGGAGTGATTACTTGTCTTTACCATTTACACAAAATGTGATAACAGAGACGTTAAGGTTAGGAAACATAATAACAGGAGTGATGAGAAAGGCAATGAAAGACATTGAAATAAAAGGATATTTAATACCAAAAGGATGGTGTGTACTTGCATATATTAGGTCAGTTCATGTGGATGAAAACCACTTTGAAAGCCCTTACCATTTCAATCCATGGAGATGGCAAGGTAAAGACTCAAATAATTTGAATTTCAGTCCTTTTGGAGGAGGACAAAGGTTGTGCCCAGGCCTTGAATTAGCCAGATTAGAAGCTTCCATTTTCTTACATCACTTCGTTACTGAATTCAGGTAA

Protein sequence

MDSLWIFVQLITAAAFISSTIIILRNFIIPTTPLSSSSNSFTSSNNPSLPSGSLGLPFVGETLHFVSSAYSHRPDTFMDTRRRRYGKVFKSHIFGSPTIVSTDGEVNRFILQSDSKYFVPSYPKSVTELMGKSSILLINGTLHRRIHGLIGAFFKSSHLKAQITLDMQKYLHKSISTWTSTCQQNPIHIQDEAKNVCIPHLNLLIAFEVLVKTLISLESGEEMEFLKKQFNQFIAGIMAFPINIPGTTLYRSLQAKRKMVKLVEEIIEGRRKKKKIKSEKWSNNHNNNKNGVGIINDVVDVLLNDGSQQVTDEVIAHNMIDMMIPGQDSVPILITLAIKYLSDSPSALQQLTEENMELKRQKTQFGEPLKWSDYLSLPFTQNVITETLRLGNIITGVMRKAMKDIEIKGYLIPKGWCVLAYIRSVHVDENHFESPYHFNPWRWQGKDSNNLNFSPFGGGQRLCPGLELARLEASIFLHHFVTEFR*
Homology
[Close]
BLAST of CSPI03G41480 vs. ExPASy Swiss-Prot
Match: Q94IA6 (3-epi-6-deoxocathasterone 23-monooxygenase CYP90D1 OS=Arabidopsis thaliana OX=3702 GN=CYP90D1 PE=1 SV=1)

HSP 1 Score: 558.5 bits (1438), Expect = 7.3e-158
Identity = 282/444 (63.51%), Postives = 340/444 (76.58%), Query Frame = 0 

Query: 42  TSSNNPSLPSGSLGLPFVGETLHFVSSAYSHRPDTFMDTRRRRYGKVFKSHIFGSPTIVS 101
           + S+ P  P GSLG P +GET+ FVSSAYS RP++FMD RR  YG+VFKSHIFG+ TIVS
Sbjct: 44  SQSHGPKFPHGSLGWPVIGETIEFVSSAYSDRPESFMDKRRLMYGRVFKSHIFGTATIVS 103

Query: 102 TDGEVNRFILQSDSKYFVPSYPKSVTELMGKSSILLINGTLHRRIHGLIGAFFKSSHLKA 161
           TD EVNR +LQSDS  FVP YPK+V ELMGKSSILLING+LHRR HGL+G+F KS  LKA
Sbjct: 104 TDAEVNRAVLQSDSTAFVPFYPKTVRELMGKSSILLINGSLHRRFHGLVGSFLKSPLLKA 163

Query: 162 QITLDMQKYLHKSISTWTSTCQQNPIHIQDEAKNVCIPHLNLLIAFEVLVKTLISLESGE 221
           QI  DM K+L +S+  W+   +  P+ +QD +K V         AF+VL K LIS+E GE
Sbjct: 164 QIVRDMHKFLSESMDLWS---EDQPVLLQDVSKTV---------AFKVLAKALISVEKGE 223

Query: 222 EMEFLKKQFNQFIAGIMAFPINIPGTTLYRSLQAKRKMVKLVEEIIEGRRKKKKIKSEKW 281
           ++E LK++F  FI+G+M+ PIN PGT L+RSLQAK+ MVK VE IIEG+ +K K      
Sbjct: 224 DLEELKREFENFISGLMSLPINFPGTQLHRSLQAKKNMVKQVERIIEGKIRKTK------ 283

Query: 282 SNNHNNNKNGVGIINDVVDVLLNDGSQQVTDEVIAHNMIDMMIPGQDSVPILITLAIKYL 341
                N +    I  DVVDVLL D S+ +T  +IA+NMIDMMIPG DSVP+LITLA+K+L
Sbjct: 284 -----NKEEDDVIAKDVVDVLLKDSSEHLTHNLIANNMIDMMIPGHDSVPVLITLAVKFL 343

Query: 342 SDSPSALQQLTEENMELKRQKTQFGEPLKWSDYLSLPFTQNVITETLRLGNIITGVMRKA 401
           SDSP+AL  LTEENM+LK  K   GEPL W+DYLSLPFTQ VITETLR+GN+I GVMRKA
Sbjct: 344 SDSPAALNLLTEENMKLKSLKELTGEPLYWNDYLSLPFTQKVITETLRMGNVIIGVMRKA 403

Query: 402 MKDIEIKGYLIPKGWCVLAYIRSVHVDENHFESPYHFNPWRWQGKDSNNLNFSPFGGGQR 461
           MKD+EIKGY+IPKGWC LAY+RSVH+D+ ++ESPY FNPWRWQ +D N  +FSPFGGGQR
Sbjct: 404 MKDVEIKGYVIPKGWCFLAYLRSVHLDKLYYESPYKFNPWRWQERDMNTSSFSPFGGGQR 463

Query: 462 LCPGLELARLEASIFLHHFVTEFR 486
           LCPGL+LARLE S+FLHH VT FR
Sbjct: 464 LCPGLDLARLETSVFLHHLVTRFR 464

[Close]
BLAST of CSPI03G41480 vs. ExPASy Swiss-Prot
Match: Q94IW5 (Cytochrome P450 90D2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP90D2 PE=1 SV=1)

HSP 1 Score: 525.8 bits (1353), Expect = 5.2e-148
Identity = 258/438 (58.90%), Postives = 337/438 (76.94%), Query Frame = 0 

Query: 49  LPSGSLGLPFVGETLHFVSSAYSHRPDTFMDTRRRRYGK-VFKSHIFGSPTIVSTDGEVN 108
           LP GS G P VGETL FVS AYS RP+ F+D RR+ +G  VF+SH+FGS T+V+ D EV+
Sbjct: 49  LPPGSFGWPVVGETLEFVSCAYSPRPEAFVDKRRKLHGSAVFRSHLFGSATVVTADAEVS 108

Query: 109 RFILQSDSKYFVPSYPKSVTELMGKSSILLINGTLHRRIHGLIGAFFKSSHLKAQITLDM 168
           RF+LQSD++ FVP YP+S+TELMGKSSILLING L RR+HGL+GAFFKSSHLK+Q+T DM
Sbjct: 109 RFVLQSDARAFVPWYPRSLTELMGKSSILLINGALQRRVHGLVGAFFKSSHLKSQLTADM 168

Query: 169 QKYLHKSISTWTSTCQQNPIHIQDEAKNVCIPHLNLLIAFEVLVKTLISLESGEEMEFLK 228
           ++ L  ++S++  +   + +H+Q  AK+V          FE+LV+ LI LE+GEEM+ LK
Sbjct: 169 RRRLSPALSSFPDS---SLLHVQHLAKSV---------VFEILVRGLIGLEAGEEMQQLK 228

Query: 229 KQFNQFIAGIMAFPINIPGTTLYRSLQAKRKMVKLVEEIIEGRRKKKKIKSEKWSNNHNN 288
           +QF +FI G+M+ PI +PGT LYRSLQAK+KM +L++ II  +R ++   S         
Sbjct: 229 QQFQEFIVGLMSLPIKLPGTRLYRSLQAKKKMARLIQRIIREKRARRAAASPP------- 288

Query: 289 NKNGVGIINDVVDVLLNDGSQQVTDEVIAHNMIDMMIPGQDSVPILITLAIKYLSDSPSA 348
                    D +DVL+ DGS ++TDE+I+ NMID+MIP +DSVP+LITLA+K+LS+ P A
Sbjct: 289 --------RDAIDVLIGDGSDELTDELISDNMIDLMIPAEDSVPVLITLAVKFLSECPLA 348

Query: 349 LQQLTEENMELKRQKTQFGEPLKWSDYLSLPFTQNVITETLRLGNIITGVMRKAMKDIEI 408
           L QL EEN++LKR+KT  GE L+W+DY+SL FTQ+VITETLRLGNII G+MRKA++D+E+
Sbjct: 349 LHQLEEENIQLKRRKTDMGETLQWTDYMSLSFTQHVITETLRLGNIIGGIMRKAVRDVEV 408

Query: 409 KGYLIPKGWCVLAYIRSVHVDENHFESPYHFNPWRWQGKDSNNLNFSPFGGGQRLCPGLE 468
           KG+LIPKGWCV  Y RSVH+D+  ++ PY FNPWRW+ KD +N +F+PFGGGQRLCPGL+
Sbjct: 409 KGHLIPKGWCVFVYFRSVHLDDTLYDEPYKFNPWRWKEKDMSNGSFTPFGGGQRLCPGLD 459

Query: 469 LARLEASIFLHHFVTEFR 486
           LARLEASIFLHH VT FR
Sbjct: 469 LARLEASIFLHHLVTSFR 459

[Close]
BLAST of CSPI03G41480 vs. ExPASy Swiss-Prot
Match: Q9M066 (3-epi-6-deoxocathasterone 23-monooxygenase CYP90C1 OS=Arabidopsis thaliana OX=3702 GN=CYP90C1 PE=1 SV=3)

HSP 1 Score: 455.3 bits (1170), Expect = 8.7e-127
Identity = 225/443 (50.79%), Postives = 313/443 (70.65%), Query Frame = 0 

Query: 49  LPSGSLGLPFVGETLHFVSSAYSHRPDTFMDTRRRRYGKVFKSHIFGSPTIVSTDGEVNR 108
           +P+GSLG P +GETL+F++  YS RP TFMD R+  YGKVFK++I G+P I+STD EVN+
Sbjct: 69  IPNGSLGWPVIGETLNFIACGYSSRPVTFMDKRKSLYGKVFKTNIIGTPIIISTDAEVNK 128

Query: 109 FILQSDSKYFVPSYPKSVTELMGKSSILLINGTLHRRIHGLIGAFFKSSHLKAQITLDMQ 168
            +LQ+    FVP+YPKS+TEL+G++SIL ING   +R+H LIGAF +S HLK +IT D++
Sbjct: 129 VVLQNHGNTFVPAYPKSITELLGENSILSINGPHQKRLHTLIGAFLRSPHLKDRITRDIE 188

Query: 169 KYLHKSISTWTSTCQQNPIHIQDEAKNVCIPHLNLLIAFEVLVKTLISLESGEEMEFLKK 228
             +  ++++W    Q   +H+QDE K          + FE+LVK L+S   GE+M  LK 
Sbjct: 189 ASVVLTLASW---AQLPLVHVQDEIKK---------MTFEILVKVLMSTSPGEDMNILKL 248

Query: 229 QFNQFIAGIMAFPINIPGTTLYRSLQAKRKMVKLVEEIIEGRRKKKKIKSEKWSNNHNNN 288
           +F +FI G++  PI  PGT LY+SL+AK +++K+V++++E R+      S          
Sbjct: 249 EFEEFIKGLICIPIKFPGTRLYKSLKAKERLIKMVKKVVEERQVAMTTTSP--------- 308

Query: 289 KNGVGIINDVVDVLLNDG----SQQVTDEVIAHNMIDMMIPGQDSVPILITLAIKYLSDS 348
                  NDVVDVLL DG     Q    + ++  +++MMIPG++++P  +TLA+K+LSD+
Sbjct: 309 ------ANDVVDVLLRDGGDSEKQSQPSDFVSGKIVEMMIPGEETMPTAMTLAVKFLSDN 368

Query: 349 PSALQQLTEENMELKRQKTQFGEPLKWSDYLSLPFTQNVITETLRLGNIITGVMRKAMKD 408
           P AL +L EENME+KR+K + GE  KW+DY+SL FTQNVI ETLR+ NII GV RKA+KD
Sbjct: 369 PVALAKLVEENMEMKRRKLELGEEYKWTDYMSLSFTQNVINETLRMANIINGVWRKALKD 428

Query: 409 IEIKGYLIPKGWCVLAYIRSVHVDENHFESPYHFNPWRW---QGKDSNNLNFSPFGGGQR 468
           +EIKGYLIPKGWCVLA   SVH+DE+ +++PY F+PWRW    G  ++++ F+PFGGGQR
Sbjct: 429 VEIKGYLIPKGWCVLASFISVHMDEDIYDNPYQFDPWRWDRINGSANSSICFTPFGGGQR 484

Query: 469 LCPGLELARLEASIFLHHFVTEF 485
           LCPGLEL++LE SIFLHH VT +
Sbjct: 489 LCPGLELSKLEISIFLHHLVTRY 484

[Close]
BLAST of CSPI03G41480 vs. ExPASy Swiss-Prot
Match: Q42569 (Cytochrome P450 90A1 OS=Arabidopsis thaliana OX=3702 GN=CYP90A1 PE=1 SV=1)

HSP 1 Score: 336.7 bits (862), Expect = 4.5e-91
Identity = 182/444 (40.99%), Postives = 275/444 (61.94%), Query Frame = 0 

Query: 49  LPSGSLGLPFVGETLHFVSSAYSHRPDTFMDTRRRRYGKVFKSHIFGSPTIVSTDGEVNR 108
           LP GSLGLP +GET   + +  +  P+ F+D R  RYG VF +H+FG PTI S D E NR
Sbjct: 31  LPPGSLGLPLIGETFQLIGAYKTENPEPFIDERVARYGSVFMTHLFGEPTIFSADPETNR 90

Query: 109 FILQSDSKYFVPSYPKSVTELMGKSSILLINGTLHRRIHGLIGAFFKSSHLKAQITLDMQ 168
           F+LQ++ K F  SYP S+  L+GK S+LL+ G+LH+R+H L  +F  SS +K  + LD+ 
Sbjct: 91  FVLQNEGKLFECSYPASICNLLGKHSLLLMKGSLHKRMHSLTMSFANSSIIKDHLMLDID 150

Query: 169 KYLHKSISTWTSTCQQNPIHIQDEAKNVCIPHLNLLIAFEVLVKTLISLESGEEMEFLKK 228
           + +  ++ +W+S      + + +EAK          I FE+ VK L+S + GE  E L+K
Sbjct: 151 RLVRFNLDSWSSR-----VLLMEEAKK---------ITFELTVKQLMSFDPGEWSESLRK 210

Query: 229 QFNQFIAGIMAFPINIPGTTLYRSLQAKRKMVKLVEEIIEGRRKKKKIKSEKWSNNHNNN 288
           ++   I G  + P+ +  TT  +++QA+RK+ + +  ++  RR++++  +E+        
Sbjct: 211 EYLLVIEGFFSLPLPLFSTTYRKAIQARRKVAEALTVVVMKRREEEEEGAER-------- 270

Query: 289 KNGVGIINDVVDVLL--NDGSQQVTDEVIAHNMIDMMIPGQDSVPILITLAIKYLSDSPS 348
                   D++  LL  +DG    +DE I   ++ +++ G ++   ++TLA+K+L+++P 
Sbjct: 271 ------KKDMLAALLAADDG---FSDEEIVDFLVALLVAGYETTSTIMTLAVKFLTETPL 330

Query: 349 ALQQLTEENMELKRQKTQFGEPLKWSDYLSLPFTQNVITETLRLGNIITGVMRKAMKDIE 408
           AL QL EE+ +++  K+     L+WSDY S+PFTQ V+ ETLR+ NII GV R+AM D+E
Sbjct: 331 ALAQLKEEHEKIRAMKSD-SYSLEWSDYKSMPFTQCVVNETLRVANIIGGVFRRAMTDVE 390

Query: 409 IKGYLIPKGWCVLAYIRSVHVDENHFESPYHFNPWRWQGKDSNNLN------FSPFGGGQ 468
           IKGY IPKGW V +  R+VH+D NHF+    FNPWRWQ   SN++       F+PFGGG 
Sbjct: 391 IKGYKIPKGWKVFSSFRAVHLDPNHFKDARTFNPWRWQ---SNSVTTGPSNVFTPFGGGP 439

Query: 469 RLCPGLELARLEASIFLHHFVTEF 485
           RLCPG ELAR+  S+FLH  VT F
Sbjct: 451 RLCPGYELARVALSVFLHRLVTGF 439

[Close]
BLAST of CSPI03G41480 vs. ExPASy Swiss-Prot
Match: O64989 (Cytochrome P450 90B1 OS=Arabidopsis thaliana OX=3702 GN=CYP90B1 PE=1 SV=2)

HSP 1 Score: 289.3 bits (739), Expect = 8.3e-77
Identity = 163/463 (35.21%), Postives = 273/463 (58.96%), Query Frame = 0 

Query: 48  SLPSGSLGLPFVGETLHFVSSAYSHRPDTFMDTRRRRYGKVFKSHIFGSPTIVSTDGEVN 107
           +LP G  G PF+GET+ ++    +     FM     +YGK+++S++FG PTIVS D  +N
Sbjct: 38  NLPPGKSGWPFLGETIGYLKPYTATTLGDFMQQHVSKYGKIYRSNLFGEPTIVSADAGLN 97

Query: 108 RFILQSDSKYFVPSYPKSVTELMGKSSILLINGTLHRRIHGLIGAFFKSSHLKAQITLDM 167
           RFILQ++ + F  SYP+S+  ++GK S+L++ G +HR +  +   F   + L+  +  D+
Sbjct: 98  RFILQNEGRLFECSYPRSIGGILGKWSMLVLVGDMHRDMRSISLNFLSHARLRTILLKDV 157

Query: 168 QKYLHKSISTWTSTCQQNPI-HIQDEAKNVCIPHLNLLIAFEVLVKTLISLESG-EEMEF 227
           +++    + +W    QQN I   QDEAK            F ++ K ++S++ G EE E 
Sbjct: 158 ERHTLFVLDSW----QQNSIFSAQDEAKK---------FTFNLMAKHIMSMDPGEEETEQ 217

Query: 228 LKKQFNQFIAGIMAFPINIPGTTLYRSLQAKRKMVKLVEEIIEGRR--------KKKKIK 287
           LKK++  F+ G+++ P+N+PGT  +++LQ++  ++K +E  +E R+        +++++K
Sbjct: 218 LKKEYVTFMKGVVSAPLNLPGTAYHKALQSRATILKFIERKMEERKLDIKEEDQEEEEVK 277

Query: 288 SE---KWSNNHNNNKNGVGIINDVVDVLLNDGSQQVTDEVIAHNMIDMMIPGQDSVPILI 347
           +E   + S + +  K      +D++  +L   +  ++ E I   ++ ++  G ++  + I
Sbjct: 278 TEDEAEMSKSDHVRKQRTD--DDLLGWVLKHSN--LSTEQILDLILSLLFAGHETSSVAI 337

Query: 348 TLAIKYLSDSPSALQQLTEENMELKRQKTQFGE-PLKWSDYLSLPFTQNVITETLRLGNI 407
            LAI +L   P A+++L EE++E+ R K + GE  L W DY  + FTQ VI ETLRLGN+
Sbjct: 338 ALAIFFLQACPKAVEELREEHLEIARAKKELGESELNWDDYKKMDFTQCVINETLRLGNV 397

Query: 408 ITGVMRKAMKDIEIKGYLIPKGWCVLAYIRSVHVDENHFESPYHFNPWRWQGKDSN---- 467
           +  + RKA+KD+  KGY IP GW VL  I +VH+D + ++ P  FNPWRWQ +++     
Sbjct: 398 VRFLHRKALKDVRYKGYDIPSGWKVLPVISAVHLDNSRYDQPNLFNPWRWQQQNNGASSS 457

Query: 468 --------NLNFSPFGGGQRLCPGLELARLEASIFLHHFVTEF 485
                     N+ PFGGG RLC G ELA+LE ++F+HH V +F
Sbjct: 458 GSGSFSTWGNNYMPFGGGPRLCAGSELAKLEMAVFIHHLVLKF 483

[Close]
BLAST of CSPI03G41480 vs. ExPASy TrEMBL
Match: A0A0A0LCT5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G848830 PE=3 SV=1)

HSP 1 Score: 960.3 bits (2481), Expect = 3.1e-276
Identity = 485/485 (100.00%), Postives = 485/485 (100.00%), Query Frame = 0 

Query: 1   MDSLWIFVQLITAAAFISSTIIILRNFIIPTTPLSSSSNSFTSSNNPSLPSGSLGLPFVG 60
           MDSLWIFVQLITAAAFISSTIIILRNFIIPTTPLSSSSNSFTSSNNPSLPSGSLGLPFVG
Sbjct: 1   MDSLWIFVQLITAAAFISSTIIILRNFIIPTTPLSSSSNSFTSSNNPSLPSGSLGLPFVG 60

Query: 61  ETLHFVSSAYSHRPDTFMDTRRRRYGKVFKSHIFGSPTIVSTDGEVNRFILQSDSKYFVP 120
           ETLHFVSSAYSHRPDTFMDTRRRRYGKVFKSHIFGSPTIVSTDGEVNRFILQSDSKYFVP
Sbjct: 61  ETLHFVSSAYSHRPDTFMDTRRRRYGKVFKSHIFGSPTIVSTDGEVNRFILQSDSKYFVP 120

Query: 121 SYPKSVTELMGKSSILLINGTLHRRIHGLIGAFFKSSHLKAQITLDMQKYLHKSISTWTS 180
           SYPKSVTELMGKSSILLINGTLHRRIHGLIGAFFKSSHLKAQITLDMQKYLHKSISTWTS
Sbjct: 121 SYPKSVTELMGKSSILLINGTLHRRIHGLIGAFFKSSHLKAQITLDMQKYLHKSISTWTS 180

Query: 181 TCQQNPIHIQDEAKNVCIPHLNLLIAFEVLVKTLISLESGEEMEFLKKQFNQFIAGIMAF 240
           TCQQNPIHIQDEAKNVCIPHLNLLIAFEVLVKTLISLESGEEMEFLKKQFNQFIAGIMAF
Sbjct: 181 TCQQNPIHIQDEAKNVCIPHLNLLIAFEVLVKTLISLESGEEMEFLKKQFNQFIAGIMAF 240

Query: 241 PINIPGTTLYRSLQAKRKMVKLVEEIIEGRRKKKKIKSEKWSNNHNNNKNGVGIINDVVD 300
           PINIPGTTLYRSLQAKRKMVKLVEEIIEGRRKKKKIKSEKWSNNHNNNKNGVGIINDVVD
Sbjct: 241 PINIPGTTLYRSLQAKRKMVKLVEEIIEGRRKKKKIKSEKWSNNHNNNKNGVGIINDVVD 300

Query: 301 VLLNDGSQQVTDEVIAHNMIDMMIPGQDSVPILITLAIKYLSDSPSALQQLTEENMELKR 360
           VLLNDGSQQVTDEVIAHNMIDMMIPGQDSVPILITLAIKYLSDSPSALQQLTEENMELKR
Sbjct: 301 VLLNDGSQQVTDEVIAHNMIDMMIPGQDSVPILITLAIKYLSDSPSALQQLTEENMELKR 360

Query: 361 QKTQFGEPLKWSDYLSLPFTQNVITETLRLGNIITGVMRKAMKDIEIKGYLIPKGWCVLA 420
           QKTQFGEPLKWSDYLSLPFTQNVITETLRLGNIITGVMRKAMKDIEIKGYLIPKGWCVLA
Sbjct: 361 QKTQFGEPLKWSDYLSLPFTQNVITETLRLGNIITGVMRKAMKDIEIKGYLIPKGWCVLA 420

Query: 421 YIRSVHVDENHFESPYHFNPWRWQGKDSNNLNFSPFGGGQRLCPGLELARLEASIFLHHF 480
           YIRSVHVDENHFESPYHFNPWRWQGKDSNNLNFSPFGGGQRLCPGLELARLEASIFLHHF
Sbjct: 421 YIRSVHVDENHFESPYHFNPWRWQGKDSNNLNFSPFGGGQRLCPGLELARLEASIFLHHF 480

Query: 481 VTEFR 486
           VTEFR
Sbjct: 481 VTEFR 485

[Close]
BLAST of CSPI03G41480 vs. ExPASy TrEMBL
Match: A0A1S3BHU7 (3-epi-6-deoxocathasterone 23-monooxygenase-like OS=Cucumis melo OX=3656 GN=LOC103490016 PE=3 SV=1)

HSP 1 Score: 890.6 bits (2300), Expect = 3.0e-255
Identity = 458/486 (94.24%), Postives = 465/486 (95.68%), Query Frame = 0 

Query: 1   MDSLWIFVQLITAAAFISSTIIILRNFIIPTTPLSSSSNSFTSSNNPSLPSGSLGLPFVG 60
           MDSLWIFVQ+ITAAAFISSTIIIL+NFIIPTTP SSSSNSFTSSNNPSLPSGSLGLPFVG
Sbjct: 1   MDSLWIFVQVITAAAFISSTIIILKNFIIPTTPSSSSSNSFTSSNNPSLPSGSLGLPFVG 60

Query: 61  ETLHFVSSAYSHRPDTFMDTRRRRYGKVFKSHIFGSPTIVSTDGEVNRFILQSDSKYFVP 120
           ETLHFVSSAYSHRPDTFMDTRRRRYGKVFKSHIFGSPTIVSTDGEVNRFILQSDSKYFVP
Sbjct: 61  ETLHFVSSAYSHRPDTFMDTRRRRYGKVFKSHIFGSPTIVSTDGEVNRFILQSDSKYFVP 120

Query: 121 SYPKSVTELMGKSSILLINGTLHRRIHGLIGAFFKSSHLKAQITLDMQKYLHKSISTWTS 180
           SYPKSVTELMGKSSILLINGTLHRRIHGLIGAFFKSSHLKAQITLDMQKYL KSISTWTS
Sbjct: 121 SYPKSVTELMGKSSILLINGTLHRRIHGLIGAFFKSSHLKAQITLDMQKYLQKSISTWTS 180

Query: 181 TCQQNPIHIQDEAKNVCIPHLNLLIAFEVLVKTLISLESGEEMEFLKKQFNQFIAGIMAF 240
           TCQ NPIHIQDEAKN         IAFEVLVKTL+SLESGEEMEFLKKQFNQFIAGIMAF
Sbjct: 181 TCQHNPIHIQDEAKN---------IAFEVLVKTLVSLESGEEMEFLKKQFNQFIAGIMAF 240

Query: 241 PINIPGTTLYRSLQAKRKMVKLVEEIIEGRRKKKKIKSEK-WSNNHNNNKNGVGIINDVV 300
           PINIPGTTLYRSLQAKRKMVKLVEEIIEGRRKKKK KSEK WSNN+    NGV IINDVV
Sbjct: 241 PINIPGTTLYRSLQAKRKMVKLVEEIIEGRRKKKKKKSEKYWSNNN----NGVAIINDVV 300

Query: 301 DVLLNDGSQQVTDEVIAHNMIDMMIPGQDSVPILITLAIKYLSDSPSALQQLTEENMELK 360
           DVLLNDGSQQVTDEVIAHNMIDMMIPGQDSVPILITLAIKYLSDSPSALQQLTEENMELK
Sbjct: 301 DVLLNDGSQQVTDEVIAHNMIDMMIPGQDSVPILITLAIKYLSDSPSALQQLTEENMELK 360

Query: 361 RQKTQFGEPLKWSDYLSLPFTQNVITETLRLGNIITGVMRKAMKDIEIKGYLIPKGWCVL 420
           RQKTQ GEPLKW+DYLSLPFTQNVITETLRLGNIITGVMRKAMKDIEIKGYLIPKGWCVL
Sbjct: 361 RQKTQLGEPLKWTDYLSLPFTQNVITETLRLGNIITGVMRKAMKDIEIKGYLIPKGWCVL 420

Query: 421 AYIRSVHVDENHFESPYHFNPWRWQGKDSNNLNFSPFGGGQRLCPGLELARLEASIFLHH 480
           AYIRSVHVDEN FESPYHFNPWRWQGKDSNNLNF+PFGGGQRLCPGLELARLEASIFLHH
Sbjct: 421 AYIRSVHVDENQFESPYHFNPWRWQGKDSNNLNFTPFGGGQRLCPGLELARLEASIFLHH 473

Query: 481 FVTEFR 486
           F+TEFR
Sbjct: 481 FITEFR 473

[Close]
BLAST of CSPI03G41480 vs. ExPASy TrEMBL
Match: A0A5A7U5K3 (3-epi-6-deoxocathasterone 23-monooxygenase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold511G00920 PE=3 SV=1)

HSP 1 Score: 832.4 bits (2149), Expect = 9.7e-238
Identity = 428/485 (88.25%), Postives = 435/485 (89.69%), Query Frame = 0 

Query: 1   MDSLWIFVQLITAAAFISSTIIILRNFIIPTTPLSSSSNSFTSSNNPSLPSGSLGLPFVG 60
           MDSLWIFVQ+ITAAAFISSTIIIL+NFIIPTTP SSSSNSFTSSNNPSLPSGSLGLPFVG
Sbjct: 1   MDSLWIFVQVITAAAFISSTIIILKNFIIPTTPSSSSSNSFTSSNNPSLPSGSLGLPFVG 60

Query: 61  ETLHFVSSAYSHRPDTFMDTRRRRYGKVFKSHIFGSPTIVSTDGEVNRFILQSDSKYFVP 120
           ETLHFVSSAYSHRPDTFMDTRRRRYGKVFKSHIFGSPTIVSTDGEVNRFILQSDSKYFVP
Sbjct: 61  ETLHFVSSAYSHRPDTFMDTRRRRYGKVFKSHIFGSPTIVSTDGEVNRFILQSDSKYFVP 120

Query: 121 SYPKSVTELMGKSSILLINGTLHRRIHGLIGAFFKSSHLKAQITLDMQKYLHKSISTWTS 180
           SYPKSVTELMGKSSILLINGTLHRRIHGLIGAFFKSSHLKAQITLDMQKYL KSISTWTS
Sbjct: 121 SYPKSVTELMGKSSILLINGTLHRRIHGLIGAFFKSSHLKAQITLDMQKYLQKSISTWTS 180

Query: 181 TCQQNPIHIQDEAKNVCIPHLNLLIAFEVLVKTLISLESGEEMEFLKKQFNQFIAGIMAF 240
           TCQ NPIHIQDEAKN         IAFEVLVKTL+SLESGEEMEFLKKQFNQFIAGIMAF
Sbjct: 181 TCQHNPIHIQDEAKN---------IAFEVLVKTLVSLESGEEMEFLKKQFNQFIAGIMAF 240

Query: 241 PINIPGTTLYRSLQAKRKMVKLVEEIIEGRRKKKKIKSEKWSNNHNNNKNGVGIINDVVD 300
           PINIPGTTLYRSLQA                                    + IINDVVD
Sbjct: 241 PINIPGTTLYRSLQA------------------------------------IAIINDVVD 300

Query: 301 VLLNDGSQQVTDEVIAHNMIDMMIPGQDSVPILITLAIKYLSDSPSALQQLTEENMELKR 360
           VLLNDGSQQVTDEVIAHNMIDMMIPGQDSVPILITLAIKYLSDSPSALQQLTEENMELKR
Sbjct: 301 VLLNDGSQQVTDEVIAHNMIDMMIPGQDSVPILITLAIKYLSDSPSALQQLTEENMELKR 360

Query: 361 QKTQFGEPLKWSDYLSLPFTQNVITETLRLGNIITGVMRKAMKDIEIKGYLIPKGWCVLA 420
           QKTQ GEPLKW+DYLSLPFTQNVITETLRLGNIITGVMRKAMKDIEIKGYLIPKGWCVLA
Sbjct: 361 QKTQLGEPLKWTDYLSLPFTQNVITETLRLGNIITGVMRKAMKDIEIKGYLIPKGWCVLA 420

Query: 421 YIRSVHVDENHFESPYHFNPWRWQGKDSNNLNFSPFGGGQRLCPGLELARLEASIFLHHF 480
           YIRSVHVDENHFESPYHFNPWRWQGKDSNNLNF+PFGGGQRLCPGLELARLEASIFLHHF
Sbjct: 421 YIRSVHVDENHFESPYHFNPWRWQGKDSNNLNFTPFGGGQRLCPGLELARLEASIFLHHF 440

Query: 481 VTEFR 486
           +TEFR
Sbjct: 481 ITEFR 440

[Close]
BLAST of CSPI03G41480 vs. ExPASy TrEMBL
Match: A0A6J1GWI2 (3-epi-6-deoxocathasterone 23-monooxygenase OS=Cucurbita moschata OX=3662 GN=LOC111458064 PE=3 SV=1)

HSP 1 Score: 741.5 bits (1913), Expect = 2.2e-210
Identity = 387/485 (79.79%), Postives = 419/485 (86.39%), Query Frame = 0 

Query: 1   MDSLWIFVQLITAAAFISSTIIILRNFIIPTTPLSSSSNSFTSSNNPSLPSGSLGLPFVG 60
           MDSLWIFVQLITAAAFI S II++         L +S     SS+ PSLP+GSLGL FVG
Sbjct: 1   MDSLWIFVQLITAAAFICSIIIVI---------LKAS-----SSSKPSLPAGSLGLLFVG 60

Query: 61  ETLHFVSSAYSHRPDTFMDTRRRRYGKVFKSHIFGSPTIVSTDGEVNRFILQSDSKYFVP 120
           ETLHFVSSAYSHRPD+FMDTRR RYGKVFKSH+FGSPTIVSTD EVNRF+LQSD+K FV 
Sbjct: 61  ETLHFVSSAYSHRPDSFMDTRRLRYGKVFKSHLFGSPTIVSTDAEVNRFVLQSDAKAFVT 120

Query: 121 SYPKSVTELMGKSSILLINGTLHRRIHGLIGAFFKSSHLKAQITLDMQKYLHKSISTWTS 180
           SYPKSVT+LMGK+SILLING LHRRIHGLIGAFFKS+HLKAQ+T+DMQK+L KS+STW+S
Sbjct: 121 SYPKSVTDLMGKASILLINGPLHRRIHGLIGAFFKSAHLKAQLTIDMQKFLQKSMSTWSS 180

Query: 181 TCQQNPIHIQDEAKNVCIPHLNLLIAFEVLVKTLISLESGEEMEFLKKQFNQFIAGIMAF 240
           + Q NPIHIQDEAKN         IAFEVLVKTL+SLE GEEMEFLKKQFNQFIAGIMAF
Sbjct: 181 S-QHNPIHIQDEAKN---------IAFEVLVKTLMSLEGGEEMEFLKKQFNQFIAGIMAF 240

Query: 241 PINIPGTTLYRSLQAKRKMVKLVEEIIEGRRKKKKIKSEKWSNNHNNNKNGVGIINDVVD 300
           PINIPGTTLYRSLQAKRKMVKLVEEIIEG+RKK    +E  SNN         + NDVVD
Sbjct: 241 PINIPGTTLYRSLQAKRKMVKLVEEIIEGKRKK---MNENLSNNR--------VPNDVVD 300

Query: 301 VLLNDGSQQVTDEVIAHNMIDMMIPGQDSVPILITLAIKYLSDSPSALQQLTEENMELKR 360
           +LLNDGS QVTDE+IA N+IDMMIPGQDSVPILITLAIKYLSD P ALQ+L EENMELKR
Sbjct: 301 ILLNDGSHQVTDELIADNIIDMMIPGQDSVPILITLAIKYLSDCPLALQRLVEENMELKR 360

Query: 361 QKTQFGEPLKWSDYLSLPFTQNVITETLRLGNIITGVMRKAMKDIEIKGYLIPKGWCVLA 420
            KTQ GEPLKW+DYLSLPFTQNVITETLR+GNIITGVMRKA+KD+EIKGYLIPKGWCVL 
Sbjct: 361 LKTQLGEPLKWTDYLSLPFTQNVITETLRMGNIITGVMRKAVKDVEIKGYLIPKGWCVLV 420

Query: 421 YIRSVHVDENHFESPYHFNPWRWQGKDSNNLNFSPFGGGQRLCPGLELARLEASIFLHHF 480
           Y RSVHVDE HFESPYHFNPWRWQ KDS +LNF+PFGGGQRLCPGLELARLE SIFLHHF
Sbjct: 421 YFRSVHVDEEHFESPYHFNPWRWQDKDSTSLNFTPFGGGQRLCPGLELARLETSIFLHHF 450

Query: 481 VTEFR 486
           VTEFR
Sbjct: 481 VTEFR 450

[Close]
BLAST of CSPI03G41480 vs. ExPASy TrEMBL
Match: A0A6J1IZU1 (3-epi-6-deoxocathasterone 23-monooxygenase OS=Cucurbita maxima OX=3661 GN=LOC111479936 PE=3 SV=1)

HSP 1 Score: 737.3 bits (1902), Expect = 4.2e-209
Identity = 384/482 (79.67%), Postives = 419/482 (86.93%), Query Frame = 0 

Query: 4   LWIFVQLITAAAFISSTIIILRNFIIPTTPLSSSSNSFTSSNNPSLPSGSLGLPFVGETL 63
           +WIFVQLITAAAFI STII++         L +S     SS+ PSLPSGSLGL FVGETL
Sbjct: 1   MWIFVQLITAAAFICSTIIVI---------LKAS-----SSSKPSLPSGSLGLVFVGETL 60

Query: 64  HFVSSAYSHRPDTFMDTRRRRYGKVFKSHIFGSPTIVSTDGEVNRFILQSDSKYFVPSYP 123
           HFVSSAYSHRPD+FMDTRRRRYGKVFKSH+FGSPTIVSTD EVNRF+LQSD+K FV SYP
Sbjct: 61  HFVSSAYSHRPDSFMDTRRRRYGKVFKSHLFGSPTIVSTDAEVNRFVLQSDAKAFVTSYP 120

Query: 124 KSVTELMGKSSILLINGTLHRRIHGLIGAFFKSSHLKAQITLDMQKYLHKSISTWTSTCQ 183
           KSVT+LMGK+SILLING LHRRIHGLIGAFFKS+HLKAQ+T+DMQK+L KS+STW+S+ Q
Sbjct: 121 KSVTDLMGKASILLINGPLHRRIHGLIGAFFKSAHLKAQLTIDMQKFLQKSMSTWSSS-Q 180

Query: 184 QNPIHIQDEAKNVCIPHLNLLIAFEVLVKTLISLESGEEMEFLKKQFNQFIAGIMAFPIN 243
            NPIHIQDEAKN         IAFE+LVKTL+SLE GEEMEFLKKQFNQFIAGIMAFPIN
Sbjct: 181 HNPIHIQDEAKN---------IAFELLVKTLMSLEGGEEMEFLKKQFNQFIAGIMAFPIN 240

Query: 244 IPGTTLYRSLQAKRKMVKLVEEIIEGRRKKKKIKSEKWSNNHNNNKNGVGIINDVVDVLL 303
           IPGTTLYRSLQAKRKMVKLVEEIIEG+RKK +        N N N+    + NDVVD+LL
Sbjct: 241 IPGTTLYRSLQAKRKMVKLVEEIIEGKRKKMR-------ENMNINR----VPNDVVDILL 300

Query: 304 NDGSQQVTDEVIAHNMIDMMIPGQDSVPILITLAIKYLSDSPSALQQLTEENMELKRQKT 363
           NDGS QVTDE+IA N+IDMMIPGQDSVPILITLAIK+LSD P ALQ+L EENMELKR KT
Sbjct: 301 NDGSHQVTDELIADNIIDMMIPGQDSVPILITLAIKFLSDCPLALQRLVEENMELKRLKT 360

Query: 364 QFGEPLKWSDYLSLPFTQNVITETLRLGNIITGVMRKAMKDIEIKGYLIPKGWCVLAYIR 423
           Q GEPLKW+DYLSLPFTQNVITETLR+GNIITGVMRKA+KD+EIKGYLIPKGWCVL Y R
Sbjct: 361 QLGEPLKWTDYLSLPFTQNVITETLRMGNIITGVMRKAVKDVEIKGYLIPKGWCVLVYFR 420

Query: 424 SVHVDENHFESPYHFNPWRWQGKDSNNLNFSPFGGGQRLCPGLELARLEASIFLHHFVTE 483
           SVHVDE HFESPYHFNPWRWQ KDSN+LNF+PFGGGQRLCPGLELARLE SIFLHHFVTE
Sbjct: 421 SVHVDEEHFESPYHFNPWRWQDKDSNSLNFTPFGGGQRLCPGLELARLETSIFLHHFVTE 447

Query: 484 FR 486
           FR
Sbjct: 481 FR 447

[Close]
BLAST of CSPI03G41480 vs. NCBI nr
Match: XP_011652876.1 (3-epi-6-deoxocathasterone 23-monooxygenase CYP90D1 [Cucumis sativus] >KAE8651292.1 hypothetical protein Csa_002432 [Cucumis sativus])

HSP 1 Score: 934.1 bits (2413), Expect = 4.9e-268
Identity = 476/485 (98.14%), Postives = 476/485 (98.14%), Query Frame = 0 

Query: 1   MDSLWIFVQLITAAAFISSTIIILRNFIIPTTPLSSSSNSFTSSNNPSLPSGSLGLPFVG 60
           MDSLWIFVQLITAAAFISSTIIILRNFIIPTTPLSSSSNSFTSSNNPSLPSGSLGLPFVG
Sbjct: 1   MDSLWIFVQLITAAAFISSTIIILRNFIIPTTPLSSSSNSFTSSNNPSLPSGSLGLPFVG 60

Query: 61  ETLHFVSSAYSHRPDTFMDTRRRRYGKVFKSHIFGSPTIVSTDGEVNRFILQSDSKYFVP 120
           ETLHFVSSAYSHRPDTFMDTRRRRYGKVFKSHIFGSPTIVSTDGEVNRFILQSDSKYFVP
Sbjct: 61  ETLHFVSSAYSHRPDTFMDTRRRRYGKVFKSHIFGSPTIVSTDGEVNRFILQSDSKYFVP 120

Query: 121 SYPKSVTELMGKSSILLINGTLHRRIHGLIGAFFKSSHLKAQITLDMQKYLHKSISTWTS 180
           SYPKSVTELMGKSSILLINGTLHRRIHGLIGAFFKSSHLKAQITLDMQKYLHKSISTWTS
Sbjct: 121 SYPKSVTELMGKSSILLINGTLHRRIHGLIGAFFKSSHLKAQITLDMQKYLHKSISTWTS 180

Query: 181 TCQQNPIHIQDEAKNVCIPHLNLLIAFEVLVKTLISLESGEEMEFLKKQFNQFIAGIMAF 240
           TCQQNPIHIQDEAKN         IAFEVLVKTLISLESGEEMEFLKKQFNQFIAGIMAF
Sbjct: 181 TCQQNPIHIQDEAKN---------IAFEVLVKTLISLESGEEMEFLKKQFNQFIAGIMAF 240

Query: 241 PINIPGTTLYRSLQAKRKMVKLVEEIIEGRRKKKKIKSEKWSNNHNNNKNGVGIINDVVD 300
           PINIPGTTLYRSLQAKRKMVKLVEEIIEGRRKKKKIKSEKWSNNHNNNKNGVGIINDVVD
Sbjct: 241 PINIPGTTLYRSLQAKRKMVKLVEEIIEGRRKKKKIKSEKWSNNHNNNKNGVGIINDVVD 300

Query: 301 VLLNDGSQQVTDEVIAHNMIDMMIPGQDSVPILITLAIKYLSDSPSALQQLTEENMELKR 360
           VLLNDGSQQVTDEVIAHNMIDMMIPGQDSVPILITLAIKYLSDSPSALQQLTEENMELKR
Sbjct: 301 VLLNDGSQQVTDEVIAHNMIDMMIPGQDSVPILITLAIKYLSDSPSALQQLTEENMELKR 360

Query: 361 QKTQFGEPLKWSDYLSLPFTQNVITETLRLGNIITGVMRKAMKDIEIKGYLIPKGWCVLA 420
           QKTQFGEPLKWSDYLSLPFTQNVITETLRLGNIITGVMRKAMKDIEIKGYLIPKGWCVLA
Sbjct: 361 QKTQFGEPLKWSDYLSLPFTQNVITETLRLGNIITGVMRKAMKDIEIKGYLIPKGWCVLA 420

Query: 421 YIRSVHVDENHFESPYHFNPWRWQGKDSNNLNFSPFGGGQRLCPGLELARLEASIFLHHF 480
           YIRSVHVDENHFESPYHFNPWRWQGKDSNNLNFSPFGGGQRLCPGLELARLEASIFLHHF
Sbjct: 421 YIRSVHVDENHFESPYHFNPWRWQGKDSNNLNFSPFGGGQRLCPGLELARLEASIFLHHF 476

Query: 481 VTEFR 486
           VTEFR
Sbjct: 481 VTEFR 476

[Close]
BLAST of CSPI03G41480 vs. NCBI nr
Match: XP_008447603.1 (PREDICTED: 3-epi-6-deoxocathasterone 23-monooxygenase-like [Cucumis melo])

HSP 1 Score: 890.6 bits (2300), Expect = 6.2e-255
Identity = 458/486 (94.24%), Postives = 465/486 (95.68%), Query Frame = 0 

Query: 1   MDSLWIFVQLITAAAFISSTIIILRNFIIPTTPLSSSSNSFTSSNNPSLPSGSLGLPFVG 60
           MDSLWIFVQ+ITAAAFISSTIIIL+NFIIPTTP SSSSNSFTSSNNPSLPSGSLGLPFVG
Sbjct: 1   MDSLWIFVQVITAAAFISSTIIILKNFIIPTTPSSSSSNSFTSSNNPSLPSGSLGLPFVG 60

Query: 61  ETLHFVSSAYSHRPDTFMDTRRRRYGKVFKSHIFGSPTIVSTDGEVNRFILQSDSKYFVP 120
           ETLHFVSSAYSHRPDTFMDTRRRRYGKVFKSHIFGSPTIVSTDGEVNRFILQSDSKYFVP
Sbjct: 61  ETLHFVSSAYSHRPDTFMDTRRRRYGKVFKSHIFGSPTIVSTDGEVNRFILQSDSKYFVP 120

Query: 121 SYPKSVTELMGKSSILLINGTLHRRIHGLIGAFFKSSHLKAQITLDMQKYLHKSISTWTS 180
           SYPKSVTELMGKSSILLINGTLHRRIHGLIGAFFKSSHLKAQITLDMQKYL KSISTWTS
Sbjct: 121 SYPKSVTELMGKSSILLINGTLHRRIHGLIGAFFKSSHLKAQITLDMQKYLQKSISTWTS 180

Query: 181 TCQQNPIHIQDEAKNVCIPHLNLLIAFEVLVKTLISLESGEEMEFLKKQFNQFIAGIMAF 240
           TCQ NPIHIQDEAKN         IAFEVLVKTL+SLESGEEMEFLKKQFNQFIAGIMAF
Sbjct: 181 TCQHNPIHIQDEAKN---------IAFEVLVKTLVSLESGEEMEFLKKQFNQFIAGIMAF 240

Query: 241 PINIPGTTLYRSLQAKRKMVKLVEEIIEGRRKKKKIKSEK-WSNNHNNNKNGVGIINDVV 300
           PINIPGTTLYRSLQAKRKMVKLVEEIIEGRRKKKK KSEK WSNN+    NGV IINDVV
Sbjct: 241 PINIPGTTLYRSLQAKRKMVKLVEEIIEGRRKKKKKKSEKYWSNNN----NGVAIINDVV 300

Query: 301 DVLLNDGSQQVTDEVIAHNMIDMMIPGQDSVPILITLAIKYLSDSPSALQQLTEENMELK 360
           DVLLNDGSQQVTDEVIAHNMIDMMIPGQDSVPILITLAIKYLSDSPSALQQLTEENMELK
Sbjct: 301 DVLLNDGSQQVTDEVIAHNMIDMMIPGQDSVPILITLAIKYLSDSPSALQQLTEENMELK 360

Query: 361 RQKTQFGEPLKWSDYLSLPFTQNVITETLRLGNIITGVMRKAMKDIEIKGYLIPKGWCVL 420
           RQKTQ GEPLKW+DYLSLPFTQNVITETLRLGNIITGVMRKAMKDIEIKGYLIPKGWCVL
Sbjct: 361 RQKTQLGEPLKWTDYLSLPFTQNVITETLRLGNIITGVMRKAMKDIEIKGYLIPKGWCVL 420

Query: 421 AYIRSVHVDENHFESPYHFNPWRWQGKDSNNLNFSPFGGGQRLCPGLELARLEASIFLHH 480
           AYIRSVHVDEN FESPYHFNPWRWQGKDSNNLNF+PFGGGQRLCPGLELARLEASIFLHH
Sbjct: 421 AYIRSVHVDENQFESPYHFNPWRWQGKDSNNLNFTPFGGGQRLCPGLELARLEASIFLHH 473

Query: 481 FVTEFR 486
           F+TEFR
Sbjct: 481 FITEFR 473

[Close]
BLAST of CSPI03G41480 vs. NCBI nr
Match: KAA0051142.1 (3-epi-6-deoxocathasterone 23-monooxygenase-like [Cucumis melo var. makuwa])

HSP 1 Score: 832.4 bits (2149), Expect = 2.0e-237
Identity = 428/485 (88.25%), Postives = 435/485 (89.69%), Query Frame = 0 

Query: 1   MDSLWIFVQLITAAAFISSTIIILRNFIIPTTPLSSSSNSFTSSNNPSLPSGSLGLPFVG 60
           MDSLWIFVQ+ITAAAFISSTIIIL+NFIIPTTP SSSSNSFTSSNNPSLPSGSLGLPFVG
Sbjct: 1   MDSLWIFVQVITAAAFISSTIIILKNFIIPTTPSSSSSNSFTSSNNPSLPSGSLGLPFVG 60

Query: 61  ETLHFVSSAYSHRPDTFMDTRRRRYGKVFKSHIFGSPTIVSTDGEVNRFILQSDSKYFVP 120
           ETLHFVSSAYSHRPDTFMDTRRRRYGKVFKSHIFGSPTIVSTDGEVNRFILQSDSKYFVP
Sbjct: 61  ETLHFVSSAYSHRPDTFMDTRRRRYGKVFKSHIFGSPTIVSTDGEVNRFILQSDSKYFVP 120

Query: 121 SYPKSVTELMGKSSILLINGTLHRRIHGLIGAFFKSSHLKAQITLDMQKYLHKSISTWTS 180
           SYPKSVTELMGKSSILLINGTLHRRIHGLIGAFFKSSHLKAQITLDMQKYL KSISTWTS
Sbjct: 121 SYPKSVTELMGKSSILLINGTLHRRIHGLIGAFFKSSHLKAQITLDMQKYLQKSISTWTS 180

Query: 181 TCQQNPIHIQDEAKNVCIPHLNLLIAFEVLVKTLISLESGEEMEFLKKQFNQFIAGIMAF 240
           TCQ NPIHIQDEAKN         IAFEVLVKTL+SLESGEEMEFLKKQFNQFIAGIMAF
Sbjct: 181 TCQHNPIHIQDEAKN---------IAFEVLVKTLVSLESGEEMEFLKKQFNQFIAGIMAF 240

Query: 241 PINIPGTTLYRSLQAKRKMVKLVEEIIEGRRKKKKIKSEKWSNNHNNNKNGVGIINDVVD 300
           PINIPGTTLYRSLQA                                    + IINDVVD
Sbjct: 241 PINIPGTTLYRSLQA------------------------------------IAIINDVVD 300

Query: 301 VLLNDGSQQVTDEVIAHNMIDMMIPGQDSVPILITLAIKYLSDSPSALQQLTEENMELKR 360
           VLLNDGSQQVTDEVIAHNMIDMMIPGQDSVPILITLAIKYLSDSPSALQQLTEENMELKR
Sbjct: 301 VLLNDGSQQVTDEVIAHNMIDMMIPGQDSVPILITLAIKYLSDSPSALQQLTEENMELKR 360

Query: 361 QKTQFGEPLKWSDYLSLPFTQNVITETLRLGNIITGVMRKAMKDIEIKGYLIPKGWCVLA 420
           QKTQ GEPLKW+DYLSLPFTQNVITETLRLGNIITGVMRKAMKDIEIKGYLIPKGWCVLA
Sbjct: 361 QKTQLGEPLKWTDYLSLPFTQNVITETLRLGNIITGVMRKAMKDIEIKGYLIPKGWCVLA 420

Query: 421 YIRSVHVDENHFESPYHFNPWRWQGKDSNNLNFSPFGGGQRLCPGLELARLEASIFLHHF 480
           YIRSVHVDENHFESPYHFNPWRWQGKDSNNLNF+PFGGGQRLCPGLELARLEASIFLHHF
Sbjct: 421 YIRSVHVDENHFESPYHFNPWRWQGKDSNNLNFTPFGGGQRLCPGLELARLEASIFLHHF 440

Query: 481 VTEFR 486
           +TEFR
Sbjct: 481 ITEFR 440

[Close]
BLAST of CSPI03G41480 vs. NCBI nr
Match: XP_038905609.1 (3-epi-6-deoxocathasterone 23-monooxygenase CYP90D1-like [Benincasa hispida])

HSP 1 Score: 811.6 bits (2095), Expect = 3.6e-231
Identity = 423/489 (86.50%), Postives = 443/489 (90.59%), Query Frame = 0 

Query: 1   MDSLWIFVQLITAAAFISSTIIILRNFI-IPTTP---LSSSSNSFTSSNNPSLPSGSLGL 60
           MDSLWIFVQLITAAAF+SSTIIIL++FI IPTTP    SSSSNSF SSNNPSLPSGSLGL
Sbjct: 1   MDSLWIFVQLITAAAFMSSTIIILKSFINIPTTPSSSSSSSSNSFISSNNPSLPSGSLGL 60

Query: 61  PFVGETLHFVSSAYSHRPDTFMDTRRRRYGKVFKSHIFGSPTIVSTDGEVNRFILQSDSK 120
           PFVGETLHF+SSAYS RPDTFMDTRRRRYGKVFKSHIFGSPTIVSTD EVNRFILQSD+K
Sbjct: 61  PFVGETLHFISSAYSQRPDTFMDTRRRRYGKVFKSHIFGSPTIVSTDAEVNRFILQSDAK 120

Query: 121 YFVPSYPKSVTELMGKSSILLINGTLHRRIHGLIGAFFKSSHLKAQITLDMQKYLHKSIS 180
           +FVPSYPKSVTELMGKSSILLING+LHRRIHGLIGAFFKSSHLKAQ+T+DMQKYL KSI 
Sbjct: 121 FFVPSYPKSVTELMGKSSILLINGSLHRRIHGLIGAFFKSSHLKAQVTIDMQKYLKKSIP 180

Query: 181 TWTSTCQQNPIHIQDEAKNVCIPHLNLLIAFEVLVKTLISLESGEEMEFLKKQFNQFIAG 240
           TWT+TCQ NPI+IQDEAKN         IAFEVLVKTLISLE+G+EMEFLKKQFNQFIAG
Sbjct: 181 TWTNTCQHNPIYIQDEAKN---------IAFEVLVKTLISLEAGDEMEFLKKQFNQFIAG 240

Query: 241 IMAFPINIPGTTLYRSLQAKRKMVKLVEEIIEGRRKKKKIKSEKWSNNHNNNKNGVGIIN 300
           IMAFPINIPG+ LYRSLQAKRKMVKLV+EIIEGRRKK   K EK SN           IN
Sbjct: 241 IMAFPINIPGSRLYRSLQAKRKMVKLVQEIIEGRRKK---KGEKLSN--------AIPIN 300

Query: 301 DVVDVLLNDGSQQVTDEVIAHNMIDMMIPGQDSVPILITLAIKYLSDSPSALQQLTEENM 360
           DVVDVL+NDGSQQVTDE+IA NMIDMMIPGQDSVPILITLAIKYLSDSPSAL QL EENM
Sbjct: 301 DVVDVLVNDGSQQVTDELIADNMIDMMIPGQDSVPILITLAIKYLSDSPSALHQLVEENM 360

Query: 361 ELKRQKTQFGEPLKWSDYLSLPFTQNVITETLRLGNIITGVMRKAMKDIEIKGYLIPKGW 420
           ELKR KTQ GE LKWSDYLSLPFTQNVITETLR+GNIITGVMRK MKD+EIKGYLIPKGW
Sbjct: 361 ELKRLKTQLGETLKWSDYLSLPFTQNVITETLRIGNIITGVMRKTMKDVEIKGYLIPKGW 420

Query: 421 CVLAYIRSVHVDENHFESPYHFNPWRWQGKDSNNLNFSPFGGGQRLCPGLELARLEASIF 480
           CVLAYIRSVHVDENHFESPYHFNPWRWQ KDSNN NF+PFGGGQRLCPGLELARLEASIF
Sbjct: 421 CVLAYIRSVHVDENHFESPYHFNPWRWQDKDSNNFNFTPFGGGQRLCPGLELARLEASIF 469

Query: 481 LHHFVTEFR 486
           LHHFVTEFR
Sbjct: 481 LHHFVTEFR 469

[Close]
BLAST of CSPI03G41480 vs. NCBI nr
Match: XP_023526890.1 (3-epi-6-deoxocathasterone 23-monooxygenase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 749.2 bits (1933), Expect = 2.2e-212
Identity = 388/485 (80.00%), Postives = 420/485 (86.60%), Query Frame = 0 

Query: 1   MDSLWIFVQLITAAAFISSTIIILRNFIIPTTPLSSSSNSFTSSNNPSLPSGSLGLPFVG 60
           MDSLWIFVQLITAAAFI STII++                 +SS+ PSLPSGSLGL FVG
Sbjct: 1   MDSLWIFVQLITAAAFICSTIIVI--------------FKASSSSKPSLPSGSLGLLFVG 60

Query: 61  ETLHFVSSAYSHRPDTFMDTRRRRYGKVFKSHIFGSPTIVSTDGEVNRFILQSDSKYFVP 120
           ETLHF+SSAYSHRPD+FMDTRRRRYGKVFKSH+FGSPTIVSTD EVNRF+LQSD+K FV 
Sbjct: 61  ETLHFISSAYSHRPDSFMDTRRRRYGKVFKSHLFGSPTIVSTDAEVNRFVLQSDAKAFVT 120

Query: 121 SYPKSVTELMGKSSILLINGTLHRRIHGLIGAFFKSSHLKAQITLDMQKYLHKSISTWTS 180
           SYPKSVT+LMGK+SILLING LHRRIHGLIGAFFKS+HLKAQ+T+DMQK+L KS+STW+S
Sbjct: 121 SYPKSVTDLMGKASILLINGPLHRRIHGLIGAFFKSAHLKAQLTIDMQKFLQKSMSTWSS 180

Query: 181 TCQQNPIHIQDEAKNVCIPHLNLLIAFEVLVKTLISLESGEEMEFLKKQFNQFIAGIMAF 240
           + Q NPIHIQDEAKN         IAFEVLVKTL+SLE GEEMEFLKKQFNQFIAGIMAF
Sbjct: 181 S-QHNPIHIQDEAKN---------IAFEVLVKTLMSLEGGEEMEFLKKQFNQFIAGIMAF 240

Query: 241 PINIPGTTLYRSLQAKRKMVKLVEEIIEGRRKKKKIKSEKWSNNHNNNKNGVGIINDVVD 300
           PINIPGTTLYRSLQAKRKMVKLVEEIIEG+RKK    +E  SNN         + NDVVD
Sbjct: 241 PINIPGTTLYRSLQAKRKMVKLVEEIIEGKRKK---MNENLSNNR--------VPNDVVD 300

Query: 301 VLLNDGSQQVTDEVIAHNMIDMMIPGQDSVPILITLAIKYLSDSPSALQQLTEENMELKR 360
           +LLNDGS QVTDE+IA N+IDMMIPGQDSVPILITLAIKYLSD P ALQ+L EENMELKR
Sbjct: 301 ILLNDGSHQVTDELIADNIIDMMIPGQDSVPILITLAIKYLSDCPLALQRLVEENMELKR 360

Query: 361 QKTQFGEPLKWSDYLSLPFTQNVITETLRLGNIITGVMRKAMKDIEIKGYLIPKGWCVLA 420
            KTQ GEPLKW+DYLSLPFTQNVITETLR+GNIITGVMRKA+KD+EIKGYLIPKGWCVL 
Sbjct: 361 LKTQLGEPLKWTDYLSLPFTQNVITETLRMGNIITGVMRKAVKDVEIKGYLIPKGWCVLV 420

Query: 421 YIRSVHVDENHFESPYHFNPWRWQGKDSNNLNFSPFGGGQRLCPGLELARLEASIFLHHF 480
           Y RSVHVDE HFESPYHFNPWRWQ KDSN+LNF+PFGGGQRLCPGLELARLE SIFLHHF
Sbjct: 421 YFRSVHVDEEHFESPYHFNPWRWQDKDSNSLNFTPFGGGQRLCPGLELARLETSIFLHHF 450

Query: 481 VTEFR 486
           VTEFR
Sbjct: 481 VTEFR 450

[Close]
BLAST of CSPI03G41480 vs. TAIR 10
Match: AT3G13730.1 (cytochrome P450, family 90, subfamily D, polypeptide 1 )

HSP 1 Score: 558.5 bits (1438), Expect = 5.2e-159
Identity = 282/444 (63.51%), Postives = 340/444 (76.58%), Query Frame = 0 

Query: 42  TSSNNPSLPSGSLGLPFVGETLHFVSSAYSHRPDTFMDTRRRRYGKVFKSHIFGSPTIVS 101
           + S+ P  P GSLG P +GET+ FVSSAYS RP++FMD RR  YG+VFKSHIFG+ TIVS
Sbjct: 44  SQSHGPKFPHGSLGWPVIGETIEFVSSAYSDRPESFMDKRRLMYGRVFKSHIFGTATIVS 103

Query: 102 TDGEVNRFILQSDSKYFVPSYPKSVTELMGKSSILLINGTLHRRIHGLIGAFFKSSHLKA 161
           TD EVNR +LQSDS  FVP YPK+V ELMGKSSILLING+LHRR HGL+G+F KS  LKA
Sbjct: 104 TDAEVNRAVLQSDSTAFVPFYPKTVRELMGKSSILLINGSLHRRFHGLVGSFLKSPLLKA 163

Query: 162 QITLDMQKYLHKSISTWTSTCQQNPIHIQDEAKNVCIPHLNLLIAFEVLVKTLISLESGE 221
           QI  DM K+L +S+  W+   +  P+ +QD +K V         AF+VL K LIS+E GE
Sbjct: 164 QIVRDMHKFLSESMDLWS---EDQPVLLQDVSKTV---------AFKVLAKALISVEKGE 223

Query: 222 EMEFLKKQFNQFIAGIMAFPINIPGTTLYRSLQAKRKMVKLVEEIIEGRRKKKKIKSEKW 281
           ++E LK++F  FI+G+M+ PIN PGT L+RSLQAK+ MVK VE IIEG+ +K K      
Sbjct: 224 DLEELKREFENFISGLMSLPINFPGTQLHRSLQAKKNMVKQVERIIEGKIRKTK------ 283

Query: 282 SNNHNNNKNGVGIINDVVDVLLNDGSQQVTDEVIAHNMIDMMIPGQDSVPILITLAIKYL 341
                N +    I  DVVDVLL D S+ +T  +IA+NMIDMMIPG DSVP+LITLA+K+L
Sbjct: 284 -----NKEEDDVIAKDVVDVLLKDSSEHLTHNLIANNMIDMMIPGHDSVPVLITLAVKFL 343

Query: 342 SDSPSALQQLTEENMELKRQKTQFGEPLKWSDYLSLPFTQNVITETLRLGNIITGVMRKA 401
           SDSP+AL  LTEENM+LK  K   GEPL W+DYLSLPFTQ VITETLR+GN+I GVMRKA
Sbjct: 344 SDSPAALNLLTEENMKLKSLKELTGEPLYWNDYLSLPFTQKVITETLRMGNVIIGVMRKA 403

Query: 402 MKDIEIKGYLIPKGWCVLAYIRSVHVDENHFESPYHFNPWRWQGKDSNNLNFSPFGGGQR 461
           MKD+EIKGY+IPKGWC LAY+RSVH+D+ ++ESPY FNPWRWQ +D N  +FSPFGGGQR
Sbjct: 404 MKDVEIKGYVIPKGWCFLAYLRSVHLDKLYYESPYKFNPWRWQERDMNTSSFSPFGGGQR 463

Query: 462 LCPGLELARLEASIFLHHFVTEFR 486
           LCPGL+LARLE S+FLHH VT FR
Sbjct: 464 LCPGLDLARLETSVFLHHLVTRFR 464

[Close]
BLAST of CSPI03G41480 vs. TAIR 10
Match: AT4G36380.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 455.3 bits (1170), Expect = 6.2e-128
Identity = 225/443 (50.79%), Postives = 313/443 (70.65%), Query Frame = 0 

Query: 49  LPSGSLGLPFVGETLHFVSSAYSHRPDTFMDTRRRRYGKVFKSHIFGSPTIVSTDGEVNR 108
           +P+GSLG P +GETL+F++  YS RP TFMD R+  YGKVFK++I G+P I+STD EVN+
Sbjct: 69  IPNGSLGWPVIGETLNFIACGYSSRPVTFMDKRKSLYGKVFKTNIIGTPIIISTDAEVNK 128

Query: 109 FILQSDSKYFVPSYPKSVTELMGKSSILLINGTLHRRIHGLIGAFFKSSHLKAQITLDMQ 168
            +LQ+    FVP+YPKS+TEL+G++SIL ING   +R+H LIGAF +S HLK +IT D++
Sbjct: 129 VVLQNHGNTFVPAYPKSITELLGENSILSINGPHQKRLHTLIGAFLRSPHLKDRITRDIE 188

Query: 169 KYLHKSISTWTSTCQQNPIHIQDEAKNVCIPHLNLLIAFEVLVKTLISLESGEEMEFLKK 228
             +  ++++W    Q   +H+QDE K          + FE+LVK L+S   GE+M  LK 
Sbjct: 189 ASVVLTLASW---AQLPLVHVQDEIKK---------MTFEILVKVLMSTSPGEDMNILKL 248

Query: 229 QFNQFIAGIMAFPINIPGTTLYRSLQAKRKMVKLVEEIIEGRRKKKKIKSEKWSNNHNNN 288
           +F +FI G++  PI  PGT LY+SL+AK +++K+V++++E R+      S          
Sbjct: 249 EFEEFIKGLICIPIKFPGTRLYKSLKAKERLIKMVKKVVEERQVAMTTTSP--------- 308

Query: 289 KNGVGIINDVVDVLLNDG----SQQVTDEVIAHNMIDMMIPGQDSVPILITLAIKYLSDS 348
                  NDVVDVLL DG     Q    + ++  +++MMIPG++++P  +TLA+K+LSD+
Sbjct: 309 ------ANDVVDVLLRDGGDSEKQSQPSDFVSGKIVEMMIPGEETMPTAMTLAVKFLSDN 368

Query: 349 PSALQQLTEENMELKRQKTQFGEPLKWSDYLSLPFTQNVITETLRLGNIITGVMRKAMKD 408
           P AL +L EENME+KR+K + GE  KW+DY+SL FTQNVI ETLR+ NII GV RKA+KD
Sbjct: 369 PVALAKLVEENMEMKRRKLELGEEYKWTDYMSLSFTQNVINETLRMANIINGVWRKALKD 428

Query: 409 IEIKGYLIPKGWCVLAYIRSVHVDENHFESPYHFNPWRW---QGKDSNNLNFSPFGGGQR 468
           +EIKGYLIPKGWCVLA   SVH+DE+ +++PY F+PWRW    G  ++++ F+PFGGGQR
Sbjct: 429 VEIKGYLIPKGWCVLASFISVHMDEDIYDNPYQFDPWRWDRINGSANSSICFTPFGGGQR 484

Query: 469 LCPGLELARLEASIFLHHFVTEF 485
           LCPGLEL++LE SIFLHH VT +
Sbjct: 489 LCPGLELSKLEISIFLHHLVTRY 484

[Close]
BLAST of CSPI03G41480 vs. TAIR 10
Match: AT5G05690.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 336.7 bits (862), Expect = 3.2e-92
Identity = 182/444 (40.99%), Postives = 275/444 (61.94%), Query Frame = 0 

Query: 49  LPSGSLGLPFVGETLHFVSSAYSHRPDTFMDTRRRRYGKVFKSHIFGSPTIVSTDGEVNR 108
           LP GSLGLP +GET   + +  +  P+ F+D R  RYG VF +H+FG PTI S D E NR
Sbjct: 31  LPPGSLGLPLIGETFQLIGAYKTENPEPFIDERVARYGSVFMTHLFGEPTIFSADPETNR 90

Query: 109 FILQSDSKYFVPSYPKSVTELMGKSSILLINGTLHRRIHGLIGAFFKSSHLKAQITLDMQ 168
           F+LQ++ K F  SYP S+  L+GK S+LL+ G+LH+R+H L  +F  SS +K  + LD+ 
Sbjct: 91  FVLQNEGKLFECSYPASICNLLGKHSLLLMKGSLHKRMHSLTMSFANSSIIKDHLMLDID 150

Query: 169 KYLHKSISTWTSTCQQNPIHIQDEAKNVCIPHLNLLIAFEVLVKTLISLESGEEMEFLKK 228
           + +  ++ +W+S      + + +EAK          I FE+ VK L+S + GE  E L+K
Sbjct: 151 RLVRFNLDSWSSR-----VLLMEEAKK---------ITFELTVKQLMSFDPGEWSESLRK 210

Query: 229 QFNQFIAGIMAFPINIPGTTLYRSLQAKRKMVKLVEEIIEGRRKKKKIKSEKWSNNHNNN 288
           ++   I G  + P+ +  TT  +++QA+RK+ + +  ++  RR++++  +E+        
Sbjct: 211 EYLLVIEGFFSLPLPLFSTTYRKAIQARRKVAEALTVVVMKRREEEEEGAER-------- 270

Query: 289 KNGVGIINDVVDVLL--NDGSQQVTDEVIAHNMIDMMIPGQDSVPILITLAIKYLSDSPS 348
                   D++  LL  +DG    +DE I   ++ +++ G ++   ++TLA+K+L+++P 
Sbjct: 271 ------KKDMLAALLAADDG---FSDEEIVDFLVALLVAGYETTSTIMTLAVKFLTETPL 330

Query: 349 ALQQLTEENMELKRQKTQFGEPLKWSDYLSLPFTQNVITETLRLGNIITGVMRKAMKDIE 408
           AL QL EE+ +++  K+     L+WSDY S+PFTQ V+ ETLR+ NII GV R+AM D+E
Sbjct: 331 ALAQLKEEHEKIRAMKSD-SYSLEWSDYKSMPFTQCVVNETLRVANIIGGVFRRAMTDVE 390

Query: 409 IKGYLIPKGWCVLAYIRSVHVDENHFESPYHFNPWRWQGKDSNNLN------FSPFGGGQ 468
           IKGY IPKGW V +  R+VH+D NHF+    FNPWRWQ   SN++       F+PFGGG 
Sbjct: 391 IKGYKIPKGWKVFSSFRAVHLDPNHFKDARTFNPWRWQ---SNSVTTGPSNVFTPFGGGP 439

Query: 469 RLCPGLELARLEASIFLHHFVTEF 485
           RLCPG ELAR+  S+FLH  VT F
Sbjct: 451 RLCPGYELARVALSVFLHRLVTGF 439

[Close]
BLAST of CSPI03G41480 vs. TAIR 10
Match: AT5G05690.2 (Cytochrome P450 superfamily protein )

HSP 1 Score: 295.0 bits (754), Expect = 1.1e-79
Identity = 158/398 (39.70%), Postives = 246/398 (61.81%), Query Frame = 0 

Query: 49  LPSGSLGLPFVGETLHFVSSAYSHRPDTFMDTRRRRYGKVFKSHIFGSPTIVSTDGEVNR 108
           LP GSLGLP +GET   + +  +  P+ F+D R  RYG VF +H+FG PTI S D E NR
Sbjct: 31  LPPGSLGLPLIGETFQLIGAYKTENPEPFIDERVARYGSVFMTHLFGEPTIFSADPETNR 90

Query: 109 FILQSDSKYFVPSYPKSVTELMGKSSILLINGTLHRRIHGLIGAFFKSSHLKAQITLDMQ 168
           F+LQ++ K F  SYP S+  L+GK S+LL+ G+LH+R+H L  +F  SS +K  + LD+ 
Sbjct: 91  FVLQNEGKLFECSYPASICNLLGKHSLLLMKGSLHKRMHSLTMSFANSSIIKDHLMLDID 150

Query: 169 KYLHKSISTWTSTCQQNPIHIQDEAKNVCIPHLNLLIAFEVLVKTLISLESGEEMEFLKK 228
           + +  ++ +W+S      + + +EAK          I FE+ VK L+S + GE  E L+K
Sbjct: 151 RLVRFNLDSWSSR-----VLLMEEAKK---------ITFELTVKQLMSFDPGEWSESLRK 210

Query: 229 QFNQFIAGIMAFPINIPGTTLYRSLQAKRKMVKLVEEIIEGRRKKKKIKSEKWSNNHNNN 288
           ++   I G  + P+ +  TT  +++QA+RK+ + +  ++  RR++++  +E+        
Sbjct: 211 EYLLVIEGFFSLPLPLFSTTYRKAIQARRKVAEALTVVVMKRREEEEEGAER-------- 270

Query: 289 KNGVGIINDVVDVLL--NDGSQQVTDEVIAHNMIDMMIPGQDSVPILITLAIKYLSDSPS 348
                   D++  LL  +DG    +DE I   ++ +++ G ++   ++TLA+K+L+++P 
Sbjct: 271 ------KKDMLAALLAADDG---FSDEEIVDFLVALLVAGYETTSTIMTLAVKFLTETPL 330

Query: 349 ALQQLTEENMELKRQKTQFGEPLKWSDYLSLPFTQNVITETLRLGNIITGVMRKAMKDIE 408
           AL QL EE+ +++  K+     L+WSDY S+PFTQ V+ ETLR+ NII GV R+AM D+E
Sbjct: 331 ALAQLKEEHEKIRAMKSD-SYSLEWSDYKSMPFTQCVVNETLRVANIIGGVFRRAMTDVE 390

Query: 409 IKGYLIPKGWCVLAYIRSVHVDENHFESPYHFNPWRWQ 445
           IKGY IPKGW V +  R+VH+D NHF+    FNPWRWQ
Sbjct: 391 IKGYKIPKGWKVFSSFRAVHLDPNHFKDARTFNPWRWQ 396

[Close]
BLAST of CSPI03G41480 vs. TAIR 10
Match: AT3G50660.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 289.3 bits (739), Expect = 5.9e-78
Identity = 163/463 (35.21%), Postives = 273/463 (58.96%), Query Frame = 0 

Query: 48  SLPSGSLGLPFVGETLHFVSSAYSHRPDTFMDTRRRRYGKVFKSHIFGSPTIVSTDGEVN 107
           +LP G  G PF+GET+ ++    +     FM     +YGK+++S++FG PTIVS D  +N
Sbjct: 38  NLPPGKSGWPFLGETIGYLKPYTATTLGDFMQQHVSKYGKIYRSNLFGEPTIVSADAGLN 97

Query: 108 RFILQSDSKYFVPSYPKSVTELMGKSSILLINGTLHRRIHGLIGAFFKSSHLKAQITLDM 167
           RFILQ++ + F  SYP+S+  ++GK S+L++ G +HR +  +   F   + L+  +  D+
Sbjct: 98  RFILQNEGRLFECSYPRSIGGILGKWSMLVLVGDMHRDMRSISLNFLSHARLRTILLKDV 157

Query: 168 QKYLHKSISTWTSTCQQNPI-HIQDEAKNVCIPHLNLLIAFEVLVKTLISLESG-EEMEF 227
           +++    + +W    QQN I   QDEAK            F ++ K ++S++ G EE E 
Sbjct: 158 ERHTLFVLDSW----QQNSIFSAQDEAKK---------FTFNLMAKHIMSMDPGEEETEQ 217

Query: 228 LKKQFNQFIAGIMAFPINIPGTTLYRSLQAKRKMVKLVEEIIEGRR--------KKKKIK 287
           LKK++  F+ G+++ P+N+PGT  +++LQ++  ++K +E  +E R+        +++++K
Sbjct: 218 LKKEYVTFMKGVVSAPLNLPGTAYHKALQSRATILKFIERKMEERKLDIKEEDQEEEEVK 277

Query: 288 SE---KWSNNHNNNKNGVGIINDVVDVLLNDGSQQVTDEVIAHNMIDMMIPGQDSVPILI 347
           +E   + S + +  K      +D++  +L   +  ++ E I   ++ ++  G ++  + I
Sbjct: 278 TEDEAEMSKSDHVRKQRTD--DDLLGWVLKHSN--LSTEQILDLILSLLFAGHETSSVAI 337

Query: 348 TLAIKYLSDSPSALQQLTEENMELKRQKTQFGE-PLKWSDYLSLPFTQNVITETLRLGNI 407
            LAI +L   P A+++L EE++E+ R K + GE  L W DY  + FTQ VI ETLRLGN+
Sbjct: 338 ALAIFFLQACPKAVEELREEHLEIARAKKELGESELNWDDYKKMDFTQCVINETLRLGNV 397

Query: 408 ITGVMRKAMKDIEIKGYLIPKGWCVLAYIRSVHVDENHFESPYHFNPWRWQGKDSN---- 467
           +  + RKA+KD+  KGY IP GW VL  I +VH+D + ++ P  FNPWRWQ +++     
Sbjct: 398 VRFLHRKALKDVRYKGYDIPSGWKVLPVISAVHLDNSRYDQPNLFNPWRWQQQNNGASSS 457

Query: 468 --------NLNFSPFGGGQRLCPGLELARLEASIFLHHFVTEF 485
                     N+ PFGGG RLC G ELA+LE ++F+HH V +F
Sbjct: 458 GSGSFSTWGNNYMPFGGGPRLCAGSELAKLEMAVFIHHLVLKF 483

The following BLAST results are available for this feature:
BLAST of CSPI03G41480 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q94IA67.3e-15863.513-epi-6-deoxocathasterone 23-monooxygenase CYP90D1 OS=Arabidopsis thaliana OX=37... [more]
Q94IW55.2e-14858.90Cytochrome P450 90D2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP90D2 PE=1 SV... [more]
Q9M0668.7e-12750.793-epi-6-deoxocathasterone 23-monooxygenase CYP90C1 OS=Arabidopsis thaliana OX=37... [more]
Q425694.5e-9140.99Cytochrome P450 90A1 OS=Arabidopsis thaliana OX=3702 GN=CYP90A1 PE=1 SV=1[more]
O649898.3e-7735.21Cytochrome P450 90B1 OS=Arabidopsis thaliana OX=3702 GN=CYP90B1 PE=1 SV=2[more]
BLAST of CSPI03G41480 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LCT53.1e-276100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G848830 PE=3 SV=1[more]
A0A1S3BHU73.0e-25594.243-epi-6-deoxocathasterone 23-monooxygenase-like OS=Cucumis melo OX=3656 GN=LOC10... [more]
A0A5A7U5K39.7e-23888.253-epi-6-deoxocathasterone 23-monooxygenase-like OS=Cucumis melo var. makuwa OX=1... [more]
A0A6J1GWI22.2e-21079.793-epi-6-deoxocathasterone 23-monooxygenase OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A6J1IZU14.2e-20979.673-epi-6-deoxocathasterone 23-monooxygenase OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
BLAST of CSPI03G41480 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_011652876.14.9e-26898.143-epi-6-deoxocathasterone 23-monooxygenase CYP90D1 [Cucumis sativus] >KAE8651292... [more]
XP_008447603.16.2e-25594.24PREDICTED: 3-epi-6-deoxocathasterone 23-monooxygenase-like [Cucumis melo][more]
KAA0051142.12.0e-23788.253-epi-6-deoxocathasterone 23-monooxygenase-like [Cucumis melo var. makuwa][more]
XP_038905609.13.6e-23186.503-epi-6-deoxocathasterone 23-monooxygenase CYP90D1-like [Benincasa hispida][more]
XP_023526890.12.2e-21280.003-epi-6-deoxocathasterone 23-monooxygenase [Cucurbita pepo subsp. pepo][more]
BLAST of CSPI03G41480 vs. TAIR 10
Match NameE-valueIdentityDescription
AT3G13730.15.2e-15963.51cytochrome P450, family 90, subfamily D, polypeptide 1 [more]
AT4G36380.16.2e-12850.79Cytochrome P450 superfamily protein [more]
AT5G05690.13.2e-9240.99Cytochrome P450 superfamily protein [more]
AT5G05690.21.1e-7939.70Cytochrome P450 superfamily protein [more]
AT3G50660.15.9e-7835.21Cytochrome P450 superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 463..485
score: 31.56
coord: 453..463
score: 51.62
coord: 381..399
score: 25.36
coord: 82..101
score: 27.0
coord: 421..445
score: 27.97
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 38..485
e-value: 3.8E-93
score: 314.7
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 46..485
IPR001128Cytochrome P450PFAMPF00067p450coord: 50..485
e-value: 3.5E-54
score: 184.3
NoneNo IPR availablePANTHERPTHR24286:SF303-EPI-6-DEOXOCATHASTERONE 23-MONOOXYGENASE CYP90D1coord: 25..483
NoneNo IPR availablePANTHERPTHR24286CYTOCHROME P450 26coord: 25..483
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 456..465

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI03G41480.1CSPI03G41480.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016132 brassinosteroid biosynthetic process
biological_process GO:0010268 brassinosteroid homeostasis
biological_process GO:0016125 sterol metabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0016709 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen