CSPI03G40910 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI03G40910
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionHNHc domain-containing protein
LocationChr3: 34996255 .. 35001270 (-)
RNA-Seq ExpressionCSPI03G40910
SyntenyCSPI03G40910
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GCAGCAAATAGTTTTGGGTATAGAAAAGGGAGGAAAGAAAATCTGATGACATGCACGTGCCAGTAGAGATTAGTGGGCGACAAAAGAGAGGACTCCAATTTTGTGTTGATAAACTAAATTTTGGAAAATCAAAGATATGAAGTGAAATGTGCGGGAAAAAATATGTGGTGATGAACTTTTCCGTGCGCATGTAATGCTCTTTTCGTCTTCCCCTCAACTACTGGGTTTCAATCTCTTCATCCGATGACCAGCAATCTCCACTTCCATCTCCATTGACTGTTTCAGAAATGGCGCAATTCACTGCACACACTCGACTTAAGTTGCTGCTCAACGGAGACGGTTTACCATTCGGTTCAGAATCCAAAGATCGGTTCAGATACAAGCTCAGATCAGTCCGACCTCGGAGGTTTCCTCTCTCTCGTCCCTCTTCTTCTACTACTTCCTCTACTTCAGCTTTGAGGAAACCCACTCAGCATGCTGCCGAGGTGCGTGTTGGTGTGAGGGATGAGAGCGTTAATGGCGGTGACGACGACGTTATTGGTTTTGACTATGACTACGAGCTTGAGTCTGACGATTTGGCTTGCTTCAGAGGTCTCGTCTTGGATATTTCCTACAGGTTATTCCACTTTTCATCTAATCGATGAGTTCTTCTTCAGTGTTCCTGTAGGGAAATAATTTGAATGTTATCAAGATATTAATGGTGGTATAAATGTGATTAGTTGGGAAGTTGGTCAGAGTTTGGCGTAATTTTAGGAATTGATCAATTGTAGGACGTCGGAGAAAGCCTTATCCTAATTTCTTCTTGAACTGCATACAGTTTACCTAGTCATTTTAGAAAAAAATTGAAATCCTATTCCCAACGTTATCAAATTGATAGTCCTATCGTTATCTCTGACGAAAAATCTCAACGCTCTCCTGCTTTTTTCTTTGGGGGTGGAATTACGGGTAGAATTCGACTTCTAAACTTCTTTATTGTTGATATTGATATTGGTCTTTCGATTAGCTGTTTTACTTGGTCCTACTCCATTCAAACGAGTAAAAGATACCCAAACGCTAGTTTAGATTAAGTCAACAATTTAACAAATTTCTGATGTTGGAGCTTGAAGTGGGGCAGACATTAGTAAAAGAGAAAACATGAAGCCAGCAGCAGTACGTATATCAAATAGATTATTCCCGATTCTGTTCGAATGATCGGGGGTGAATATAGCATGTATACAAGTATTACTAGGTATAGAATTTCAAGGTAATGTGTTGTATTTTCAAGTCTTTCAAGGGATCATAGATAATTTTACCTTCTCGTTCATCCTTCAAGTAGCATATATTTTTAAATACAATTGTTAGGTATGACTGCCATTTTCACTTTGTGATCCATTTTGTTCTTGCACCATGGTTTTGGATTGTTGCTAATGTTGTCCATTGAGCACCAGGGACGACTGAAGTGATTTCGTTTTTAGATAATGTTTCATTCTCAATGTTTCACCTACTTCATTTAAGCATGAAGTAACTGACGATATTTTGGGTCCATGGCGAATATGTGCTACCATGCATTGACGAGAAAACAAACACAAACAAACTCCATTCTTTCCCAGTACTTTTTTCTTACAAGTTTTGATTCATTTCCTGCTATTTAGTCGAACAAATAGTTGACTTAATTTATGTATTGGAATGGATTGACCTTCACTTGCCTGATATACTTGTTTCATATTGCTTTTATATCTCCATATTATTTGTATACCTGGAATGGTTTGCAATTTATCTTTTGCTATTGTTTCCTTGCTTCAGGCCAGTTAACGTTGTTTGTTGGAAACGTGCAATTTGTTTGGAGTTCATGGAAAAGGTTTGGCTAATCTCTTTTATGCATTATGTATAAAAAATAGAGATAATATATAGTTCCAAAAGTGTCAATTTCATTGGTTTTAGTTTTTTATTAAAAATATGTCTAGAAGATCTGGTTAGGGAAGCTCAGGAAGTCTGTCCAGGTAGCTTTAGCTAGAGTGGAAAAGCCAAATGGAAATAACAATCCATGGGCATTTAAATTGTCACTCTCTGCAATTTTTAAATTTTTGCAAGAAGAATGTCAGTTTTAAATCTTTGATGATGGCTTTCCAAAGGTTAAATAACTCGTATCAATTGATTGAGGTTGATCAGGTAGTTTGAGTTTACTATAAATTGTTAAATAATGTTTCGATACTTTATCTCAAAGCTTTTTGCCTTCTTCAGGCTGATGTTTTGGAATACTATGACCAGACAGTGAATTCTCCAAGTGGATCCTTCTATATACCAGCAGTCTTGCGGGTATGGACAAACCATTTACCTGGTTCATTCATGGTTTTTTCCCCCTATAGAATTGCATGTTATTGGTCTTTATTTCCATAATCAGGAGAAGTTCAAGATTGACTTCATTTCTTTGACTTGAATCTCTGCCTTGTCAAAGGCTTTTGAGCTATAGCATCTCTGTTTTCAGGTTCCCCATTTATTGCAAGTTGTTAAGAGGAGAAGAATCAAGAACTCTTTGAGTCGTAAAAACATACTTTATCGGGACAATTACACTTGTCAGTAAGTTAATAAATATTCACAATTGACTAGTTTTATCCAATTTATCATTTAAATTTGAAATGGAAGAAATTATTTTGAGATGTTATCCTAATTTTATGATGGGGAACTTGTATTGAATGGATAAGGAAAATTGTAAAAAATAGAAAAATCGACAAAATATTTTCACTTCTAGAAAAAGAAAAATCAAGTGTAATAGTTTTTTCTTTTAGTGGTTTTTTTCTATTTTTTGATAAAACCCCATTATATAGTCGTTGTTCTATTAAACATAGTTTTCTATTTTAATTGTATTATTATTTTTATGACAAAAGAGGCTACAATTCACTTTGTTTCTTTTAGTGGCTTGTAATATTACTGCCCGTGGAGAACAGCGAGAAAGAGTGTCTATGGCCTTGTTTTCAACCATCGACCAATATTTTATTTTGAAATAAAAACCCTTATGTTTCTTGACCCATTTCCAGTCATGCAATTGCACTAGCTTCCATTCTAGCATAAATTCATGTGTTTTTACAGCAAACTGCCTGCCTATTACATAGTGTCACCATTTTGAATGGCTAATACAATCCTCATCAACTCTCATTTATGAATTGGTTTCTGGCAAGGTCATTTTTTTACCACACTTGGATAAAACAAGCAGTCATTCGGTTGGTATTAGTTCTGTCATCAAGTATAAGGCCCTGTAAACTTTGGTTTCATAGCCTCTGAAGTGCGTGTGAGCTTATCTCTTTTGAGCTCTTGGCAATGGTCATTTTGCACTCATTCATGGTTTAGGTCGCTTGAGCACTTACTAAACCGATGGCTATCGGTCCCTATCCCAGATAATCAAACAGGATCTATACAACTGTGTTCCTGAGTTCTAAGGAGATAAGGATAATTCTTTCAAAGATTTCAGAGTTCCACACTGTATCTTGGCCACCTTTGGATGAGGTTCAATGTATTCCAAGTTCTTTTTTGCCAAGTGGAACTTCTAAAGATTCATGTGTAGTTTATGATAATTGAGGACAGTGAACTCTAGCTAATTGGAGTGTGGTTTCTTTGGCTTTTGAATAAAAAAGATTTGGGTAGTGCCAACTTGCAGTGTAGAAACGAAGCCCCTTATTATCTAAAATAGCTATGGAGGTTCTTGACTGAGTTTGATAAGTTTTGGTTGATTTAGGTTTTATTAAATTCTTATCACTACTTTCAATAGGAAGGTTTAACTAGAATATTAGTATTGACATGAGGGATGATATTGCATGGATATGAATGGAAAATCTTTGACTCTACTTGCGGTTTATGTATGCATTTTCTTTTTTCCTTTCTTCTGGTTTCCTCAAACGGGTTTTTTTTATCAACAGGTATTGTTCATCACACGAGAGTTTGACCATTGACCATGTTTTGCCCATATCCCGGGGAGGAGAATGGACATGGGAAAACCTGGTGAGTTATCCAGTAGATATTAGCATTATATTGCAAATTCTCTAATGAATTTAAGTTCACCAATATAATAAAATTTTCAATCATTAGAAGACTGATCTTGATCCTAAAACACAAAGATGCCCTAAGATTTTCCTATGTAATAAAACCGCACAAAAACATTTCTAATGACAGGTGCAGAAAAACTTAATTGACAAAGGAATCTTATCTGAAGCATTGATCTGCTACGATTCTTCTCAACATCTCATTTACGAGATTTGTTCAATTTTTTCTGTTTTCTATAATCTCTTGTGTCGCAATAGGTTGCTGCCTGTGTAAAATGCAATTCAAAGAAAGGCCAAAAAACCGTAGAAGAAGCAAATATGAAGCTGAAAAAAACTCCCAAGGTAATGTTTCGTTTTGTATGTAGTGAGAAATTGACTTTTTTTTTTCTTTTCTTCTCTTCTGTGGTGTCTGCCATGCTTCTTTATGTTTGAGAGAATATTATTTTACACTATTATTTTGATGTTGTTTAGTTAGGGTTATTTAAGATATAAAAGTCCATTAGGCCTGTTTTAGACTTGGTATTTAAATGTGCTTTAATCTCATTGTGATGTTGGCTAGAAACCCTAATCTAAAAATCCTAGTTTCTCTCTAACTCATTCTAGAATTTCACTTTATTATAATGTTTGATCTGTTCTGGTTGCATCTAGCTGAAGCTTATCGATTTTTACTTTGAATTTTGTAGGCTCCAAAAGATTATGATATACTTGCCATTCCTCTAACAAGTACCGCAATAAAGATGTTGAAACTGAGAAAGGGAACTCCTGAAGAATGGCGTCAATATCTGTCAAGTGAGCAATGACCATGTATGTACTTTTAAGTGGTACTTGTAAATTCTTCTTTGCACATATAGCACTAATTTCACCTCATACTTGGTTCTTCCAAGACTTCTTTATATAGCACAGCACCTTCTAAATTTAGCTTAATCTATCATTTGTTCCCATAACCATTTTGCATTTCTTTTTTCTTCACCATTGCTCTGCTTCTCTTTTTGTTCTACCAATC

mRNA sequence

GCAGCAAATAGTTTTGGGTATAGAAAAGGGAGGAAAGAAAATCTGATGACATGCACGTGCCAGTAGAGATTAGTGGGCGACAAAAGAGAGGACTCCAATTTTGTGTTGATAAACTAAATTTTGGAAAATCAAAGATATGAAGTGAAATGTGCGGGAAAAAATATGTGGTGATGAACTTTTCCGTGCGCATGTAATGCTCTTTTCGTCTTCCCCTCAACTACTGGGTTTCAATCTCTTCATCCGATGACCAGCAATCTCCACTTCCATCTCCATTGACTGTTTCAGAAATGGCGCAATTCACTGCACACACTCGACTTAAGTTGCTGCTCAACGGAGACGGTTTACCATTCGGTTCAGAATCCAAAGATCGGTTCAGATACAAGCTCAGATCAGTCCGACCTCGGAGGTTTCCTCTCTCTCGTCCCTCTTCTTCTACTACTTCCTCTACTTCAGCTTTGAGGAAACCCACTCAGCATGCTGCCGAGGTGCGTGTTGGTGTGAGGGATGAGAGCGTTAATGGCGGTGACGACGACGTTATTGGTTTTGACTATGACTACGAGCTTGAGTCTGACGATTTGGCTTGCTTCAGAGGTCTCGTCTTGGATATTTCCTACAGGCCAGTTAACGTTGTTTGTTGGAAACGTGCAATTTGTTTGGAGTTCATGGAAAAGGCTGATGTTTTGGAATACTATGACCAGACAGTGAATTCTCCAAGTGGATCCTTCTATATACCAGCAGTCTTGCGGGTTCCCCATTTATTGCAAGTTGTTAAGAGGAGAAGAATCAAGAACTCTTTGAGTCGTAAAAACATACTTTATCGGGACAATTACACTTGTCAGTATTGTTCATCACACGAGAGTTTGACCATTGACCATGTTTTGCCCATATCCCGGGGAGGAGAATGGACATGGGAAAACCTGGTTGCTGCCTGTGTAAAATGCAATTCAAAGAAAGGCCAAAAAACCGTAGAAGAAGCAAATATGAAGCTGAAAAAAACTCCCAAGGCTCCAAAAGATTATGATATACTTGCCATTCCTCTAACAAGTACCGCAATAAAGATGTTGAAACTGAGAAAGGGAACTCCTGAAGAATGGCGTCAATATCTGTCAAGTGAGCAATGACCATGTATGTACTTTTAAGTGGTACTTGTAAATTCTTCTTTGCACATATAGCACTAATTTCACCTCATACTTGGTTCTTCCAAGACTTCTTTATATAGCACAGCACCTTCTAAATTTAGCTTAATCTATCATTTGTTCCCATAACCATTTTGCATTTCTTTTTTCTTCACCATTGCTCTGCTTCTCTTTTTGTTCTACCAATC

Coding sequence (CDS)

ATGGCGCAATTCACTGCACACACTCGACTTAAGTTGCTGCTCAACGGAGACGGTTTACCATTCGGTTCAGAATCCAAAGATCGGTTCAGATACAAGCTCAGATCAGTCCGACCTCGGAGGTTTCCTCTCTCTCGTCCCTCTTCTTCTACTACTTCCTCTACTTCAGCTTTGAGGAAACCCACTCAGCATGCTGCCGAGGTGCGTGTTGGTGTGAGGGATGAGAGCGTTAATGGCGGTGACGACGACGTTATTGGTTTTGACTATGACTACGAGCTTGAGTCTGACGATTTGGCTTGCTTCAGAGGTCTCGTCTTGGATATTTCCTACAGGCCAGTTAACGTTGTTTGTTGGAAACGTGCAATTTGTTTGGAGTTCATGGAAAAGGCTGATGTTTTGGAATACTATGACCAGACAGTGAATTCTCCAAGTGGATCCTTCTATATACCAGCAGTCTTGCGGGTTCCCCATTTATTGCAAGTTGTTAAGAGGAGAAGAATCAAGAACTCTTTGAGTCGTAAAAACATACTTTATCGGGACAATTACACTTGTCAGTATTGTTCATCACACGAGAGTTTGACCATTGACCATGTTTTGCCCATATCCCGGGGAGGAGAATGGACATGGGAAAACCTGGTTGCTGCCTGTGTAAAATGCAATTCAAAGAAAGGCCAAAAAACCGTAGAAGAAGCAAATATGAAGCTGAAAAAAACTCCCAAGGCTCCAAAAGATTATGATATACTTGCCATTCCTCTAACAAGTACCGCAATAAAGATGTTGAAACTGAGAAAGGGAACTCCTGAAGAATGGCGTCAATATCTGTCAAGTGAGCAATGA

Protein sequence

MAQFTAHTRLKLLLNGDGLPFGSESKDRFRYKLRSVRPRRFPLSRPSSSTTSSTSALRKPTQHAAEVRVGVRDESVNGGDDDVIGFDYDYELESDDLACFRGLVLDISYRPVNVVCWKRAICLEFMEKADVLEYYDQTVNSPSGSFYIPAVLRVPHLLQVVKRRRIKNSLSRKNILYRDNYTCQYCSSHESLTIDHVLPISRGGEWTWENLVAACVKCNSKKGQKTVEEANMKLKKTPKAPKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSSEQ*
Homology
BLAST of CSPI03G40910 vs. ExPASy TrEMBL
Match: A0A0A0LG99 (HNHc domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G844900 PE=4 SV=1)

HSP 1 Score: 561.6 bits (1446), Expect = 1.8e-156
Identity = 276/277 (99.64%), Postives = 277/277 (100.00%), Query Frame = 0

Query: 1   MAQFTAHTRLKLLLNGDGLPFGSESKDRFRYKLRSVRPRRFPLSRPSSSTTSSTSALRKP 60
           MAQFTAHTRLKLLLNGDGLPFGSESKDRFRYKLRSVRPRRFPLSRPSSSTTSSTSALRKP
Sbjct: 1   MAQFTAHTRLKLLLNGDGLPFGSESKDRFRYKLRSVRPRRFPLSRPSSSTTSSTSALRKP 60

Query: 61  TQHAAEVRVGVRDESVNGGDDDVIGFDYDYELESDDLACFRGLVLDISYRPVNVVCWKRA 120
           TQHAAEVRVGVRDESVNGGDDDV+GFDYDYELESDDLACFRGLVLDISYRPVNVVCWKRA
Sbjct: 61  TQHAAEVRVGVRDESVNGGDDDVVGFDYDYELESDDLACFRGLVLDISYRPVNVVCWKRA 120

Query: 121 ICLEFMEKADVLEYYDQTVNSPSGSFYIPAVLRVPHLLQVVKRRRIKNSLSRKNILYRDN 180
           ICLEFMEKADVLEYYDQTVNSPSGSFYIPAVLRVPHLLQVVKRRRIKNSLSRKNILYRDN
Sbjct: 121 ICLEFMEKADVLEYYDQTVNSPSGSFYIPAVLRVPHLLQVVKRRRIKNSLSRKNILYRDN 180

Query: 181 YTCQYCSSHESLTIDHVLPISRGGEWTWENLVAACVKCNSKKGQKTVEEANMKLKKTPKA 240
           YTCQYCSSHESLTIDHVLPISRGGEWTWENLVAACVKCNSKKGQKTVEEANMKLKKTPKA
Sbjct: 181 YTCQYCSSHESLTIDHVLPISRGGEWTWENLVAACVKCNSKKGQKTVEEANMKLKKTPKA 240

Query: 241 PKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSSEQ 278
           PKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSSEQ
Sbjct: 241 PKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSSEQ 277

BLAST of CSPI03G40910 vs. ExPASy TrEMBL
Match: A0A5D3E2H2 (HNH endonuclease OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold734G00280 PE=4 SV=1)

HSP 1 Score: 533.5 bits (1373), Expect = 5.3e-148
Identity = 265/277 (95.67%), Postives = 269/277 (97.11%), Query Frame = 0

Query: 1   MAQFTAHTRLKLLLNGDGLPFGSESKDRFRYKLRSVRPRRFPLSRPSSSTTSSTSALRKP 60
           MAQFTAH+R+KLLLNGDGLP GSESKDRFRYKLRSVR RRFPLS PSSS TSSTSALRK 
Sbjct: 1   MAQFTAHSRVKLLLNGDGLPCGSESKDRFRYKLRSVRARRFPLSAPSSS-TSSTSALRKS 60

Query: 61  TQHAAEVRVGVRDESVNGGDDDVIGFDYDYELESDDLACFRGLVLDISYRPVNVVCWKRA 120
           TQHAAEVRVGVRDESV GGDD ++GFDYDYE ESDDLACFRGLVLDISYRPVNVVCWKRA
Sbjct: 61  TQHAAEVRVGVRDESVTGGDDAIVGFDYDYEFESDDLACFRGLVLDISYRPVNVVCWKRA 120

Query: 121 ICLEFMEKADVLEYYDQTVNSPSGSFYIPAVLRVPHLLQVVKRRRIKNSLSRKNILYRDN 180
           ICLEFMEKADVLEYYDQTVNSPSGSFYIPAVLRVPHLLQVVKRRRIKNSLSRKNILYRDN
Sbjct: 121 ICLEFMEKADVLEYYDQTVNSPSGSFYIPAVLRVPHLLQVVKRRRIKNSLSRKNILYRDN 180

Query: 181 YTCQYCSSHESLTIDHVLPISRGGEWTWENLVAACVKCNSKKGQKTVEEANMKLKKTPKA 240
           YTCQYCSSHESLTIDHVLPISRGGEWTWENLVAACVKCNSKKGQKTVEEANMKLKKTPKA
Sbjct: 181 YTCQYCSSHESLTIDHVLPISRGGEWTWENLVAACVKCNSKKGQKTVEEANMKLKKTPKA 240

Query: 241 PKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSSEQ 278
           PKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSSEQ
Sbjct: 241 PKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSSEQ 276

BLAST of CSPI03G40910 vs. ExPASy TrEMBL
Match: A0A1S3CNK0 (uncharacterized protein LOC103502982 OS=Cucumis melo OX=3656 GN=LOC103502982 PE=4 SV=1)

HSP 1 Score: 533.5 bits (1373), Expect = 5.3e-148
Identity = 265/277 (95.67%), Postives = 269/277 (97.11%), Query Frame = 0

Query: 1   MAQFTAHTRLKLLLNGDGLPFGSESKDRFRYKLRSVRPRRFPLSRPSSSTTSSTSALRKP 60
           MAQFTAH+R+KLLLNGDGLP GSESKDRFRYKLRSVR RRFPLS PSSS TSSTSALRK 
Sbjct: 1   MAQFTAHSRVKLLLNGDGLPCGSESKDRFRYKLRSVRARRFPLSAPSSS-TSSTSALRKS 60

Query: 61  TQHAAEVRVGVRDESVNGGDDDVIGFDYDYELESDDLACFRGLVLDISYRPVNVVCWKRA 120
           TQHAAEVRVGVRDESV GGDD ++GFDYDYE ESDDLACFRGLVLDISYRPVNVVCWKRA
Sbjct: 61  TQHAAEVRVGVRDESVTGGDDAIVGFDYDYEFESDDLACFRGLVLDISYRPVNVVCWKRA 120

Query: 121 ICLEFMEKADVLEYYDQTVNSPSGSFYIPAVLRVPHLLQVVKRRRIKNSLSRKNILYRDN 180
           ICLEFMEKADVLEYYDQTVNSPSGSFYIPAVLRVPHLLQVVKRRRIKNSLSRKNILYRDN
Sbjct: 121 ICLEFMEKADVLEYYDQTVNSPSGSFYIPAVLRVPHLLQVVKRRRIKNSLSRKNILYRDN 180

Query: 181 YTCQYCSSHESLTIDHVLPISRGGEWTWENLVAACVKCNSKKGQKTVEEANMKLKKTPKA 240
           YTCQYCSSHESLTIDHVLPISRGGEWTWENLVAACVKCNSKKGQKTVEEANMKLKKTPKA
Sbjct: 181 YTCQYCSSHESLTIDHVLPISRGGEWTWENLVAACVKCNSKKGQKTVEEANMKLKKTPKA 240

Query: 241 PKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSSEQ 278
           PKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSSEQ
Sbjct: 241 PKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSSEQ 276

BLAST of CSPI03G40910 vs. ExPASy TrEMBL
Match: A0A6J1DFU6 (uncharacterized protein LOC111020122 OS=Momordica charantia OX=3673 GN=LOC111020122 PE=4 SV=1)

HSP 1 Score: 484.6 bits (1246), Expect = 2.8e-133
Identity = 246/280 (87.86%), Postives = 255/280 (91.07%), Query Frame = 0

Query: 1   MAQFTAHTRLKLLLNGDGLPFGSESKDRFRYKLRSVRPRRFPLSRPS---SSTTSSTSAL 60
           MAQFT   R+KLLLNGDG+PFGSESKDR RYKLRSV  RR PLS  S   S + SS SAL
Sbjct: 1   MAQFTTLNRVKLLLNGDGVPFGSESKDRLRYKLRSV--RRIPLSAASTGISPSASSASAL 60

Query: 61  RKPTQHAAEVRVGVRDESVNGGDDDVIGFDYDYELESDDLACFRGLVLDISYRPVNVVCW 120
           RK  QH AE+RVGVRDESV+  D  ++G D DYE ESDDLACFRGLVLDISYRPVNVVCW
Sbjct: 61  RKSAQHVAEMRVGVRDESVS-DDGAIVGLDCDYEFESDDLACFRGLVLDISYRPVNVVCW 120

Query: 121 KRAICLEFMEKADVLEYYDQTVNSPSGSFYIPAVLRVPHLLQVVKRRRIKNSLSRKNILY 180
           KRAICLEFMEKADVLEYYDQTVNSPSGSFYIPAVLRVPHLLQVVKRRRIKNSLSRKNILY
Sbjct: 121 KRAICLEFMEKADVLEYYDQTVNSPSGSFYIPAVLRVPHLLQVVKRRRIKNSLSRKNILY 180

Query: 181 RDNYTCQYCSSHESLTIDHVLPISRGGEWTWENLVAACVKCNSKKGQKTVEEANMKLKKT 240
           RDNYTCQYCSSH+SLTIDHVLPISRGGEWTWENLVAACVKCNSKKGQKTVEEANMKLKKT
Sbjct: 181 RDNYTCQYCSSHDSLTIDHVLPISRGGEWTWENLVAACVKCNSKKGQKTVEEANMKLKKT 240

Query: 241 PKAPKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSSEQ 278
           PKAPKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLS+EQ
Sbjct: 241 PKAPKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSNEQ 277

BLAST of CSPI03G40910 vs. ExPASy TrEMBL
Match: A0A6J1F2H9 (uncharacterized protein LOC111439170 OS=Cucurbita moschata OX=3662 GN=LOC111439170 PE=4 SV=1)

HSP 1 Score: 474.6 bits (1220), Expect = 2.9e-130
Identity = 244/283 (86.22%), Postives = 256/283 (90.46%), Query Frame = 0

Query: 1   MAQFTAHTRLKLLLNGDGLPFGSESKDRFRYKLRSVR--PRRFPLSRPSSS----TTSST 60
           MAQFTAH+R+KLLLNGDG+PFGSE KDR R+KLRSVR   RR PLS  SS+    ++SS 
Sbjct: 1   MAQFTAHSRVKLLLNGDGVPFGSEPKDRSRHKLRSVRTLKRRTPLSGASSTGLSPSSSSA 60

Query: 61  SALRKPTQHAAEVRVGVRDESVNGGDDDVIGFDYDYELESDDLACFRGLVLDISYRPVNV 120
           SALRK  Q     RVGVR ESV+ GDD ++  DYDYE ESDDLACFRGLVLDISYRPVNV
Sbjct: 61  SALRKSAQ-----RVGVRGESVS-GDDAILDIDYDYEFESDDLACFRGLVLDISYRPVNV 120

Query: 121 VCWKRAICLEFMEKADVLEYYDQTVNSPSGSFYIPAVLRVPHLLQVVKRRRIKNSLSRKN 180
           VCWKRAICLEFMEKADVLEYYDQTV+SPSGSFYIPAVLRVPHLLQVVKRRRIK SLSRKN
Sbjct: 121 VCWKRAICLEFMEKADVLEYYDQTVSSPSGSFYIPAVLRVPHLLQVVKRRRIKQSLSRKN 180

Query: 181 ILYRDNYTCQYCSSHESLTIDHVLPISRGGEWTWENLVAACVKCNSKKGQKTVEEANMKL 240
           ILYRDNYTCQYCSSHESLTIDHVLP+SRGGEWTWENLVAACVKCNSKKGQKTVEEANMKL
Sbjct: 181 ILYRDNYTCQYCSSHESLTIDHVLPVSRGGEWTWENLVAACVKCNSKKGQKTVEEANMKL 240

Query: 241 KKTPKAPKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSSEQ 278
           KKTPKAPKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSSEQ
Sbjct: 241 KKTPKAPKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSSEQ 277

BLAST of CSPI03G40910 vs. NCBI nr
Match: XP_004141149.1 (uncharacterized protein LOC101207660 [Cucumis sativus] >XP_031738556.1 uncharacterized protein LOC101207660 [Cucumis sativus] >KGN59772.1 hypothetical protein Csa_001958 [Cucumis sativus])

HSP 1 Score: 561.6 bits (1446), Expect = 3.8e-156
Identity = 276/277 (99.64%), Postives = 277/277 (100.00%), Query Frame = 0

Query: 1   MAQFTAHTRLKLLLNGDGLPFGSESKDRFRYKLRSVRPRRFPLSRPSSSTTSSTSALRKP 60
           MAQFTAHTRLKLLLNGDGLPFGSESKDRFRYKLRSVRPRRFPLSRPSSSTTSSTSALRKP
Sbjct: 1   MAQFTAHTRLKLLLNGDGLPFGSESKDRFRYKLRSVRPRRFPLSRPSSSTTSSTSALRKP 60

Query: 61  TQHAAEVRVGVRDESVNGGDDDVIGFDYDYELESDDLACFRGLVLDISYRPVNVVCWKRA 120
           TQHAAEVRVGVRDESVNGGDDDV+GFDYDYELESDDLACFRGLVLDISYRPVNVVCWKRA
Sbjct: 61  TQHAAEVRVGVRDESVNGGDDDVVGFDYDYELESDDLACFRGLVLDISYRPVNVVCWKRA 120

Query: 121 ICLEFMEKADVLEYYDQTVNSPSGSFYIPAVLRVPHLLQVVKRRRIKNSLSRKNILYRDN 180
           ICLEFMEKADVLEYYDQTVNSPSGSFYIPAVLRVPHLLQVVKRRRIKNSLSRKNILYRDN
Sbjct: 121 ICLEFMEKADVLEYYDQTVNSPSGSFYIPAVLRVPHLLQVVKRRRIKNSLSRKNILYRDN 180

Query: 181 YTCQYCSSHESLTIDHVLPISRGGEWTWENLVAACVKCNSKKGQKTVEEANMKLKKTPKA 240
           YTCQYCSSHESLTIDHVLPISRGGEWTWENLVAACVKCNSKKGQKTVEEANMKLKKTPKA
Sbjct: 181 YTCQYCSSHESLTIDHVLPISRGGEWTWENLVAACVKCNSKKGQKTVEEANMKLKKTPKA 240

Query: 241 PKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSSEQ 278
           PKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSSEQ
Sbjct: 241 PKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSSEQ 277

BLAST of CSPI03G40910 vs. NCBI nr
Match: XP_008465340.1 (PREDICTED: uncharacterized protein LOC103502982 [Cucumis melo] >KAA0051262.1 HNH endonuclease [Cucumis melo var. makuwa] >TYK29998.1 HNH endonuclease [Cucumis melo var. makuwa])

HSP 1 Score: 533.5 bits (1373), Expect = 1.1e-147
Identity = 265/277 (95.67%), Postives = 269/277 (97.11%), Query Frame = 0

Query: 1   MAQFTAHTRLKLLLNGDGLPFGSESKDRFRYKLRSVRPRRFPLSRPSSSTTSSTSALRKP 60
           MAQFTAH+R+KLLLNGDGLP GSESKDRFRYKLRSVR RRFPLS PSSS TSSTSALRK 
Sbjct: 1   MAQFTAHSRVKLLLNGDGLPCGSESKDRFRYKLRSVRARRFPLSAPSSS-TSSTSALRKS 60

Query: 61  TQHAAEVRVGVRDESVNGGDDDVIGFDYDYELESDDLACFRGLVLDISYRPVNVVCWKRA 120
           TQHAAEVRVGVRDESV GGDD ++GFDYDYE ESDDLACFRGLVLDISYRPVNVVCWKRA
Sbjct: 61  TQHAAEVRVGVRDESVTGGDDAIVGFDYDYEFESDDLACFRGLVLDISYRPVNVVCWKRA 120

Query: 121 ICLEFMEKADVLEYYDQTVNSPSGSFYIPAVLRVPHLLQVVKRRRIKNSLSRKNILYRDN 180
           ICLEFMEKADVLEYYDQTVNSPSGSFYIPAVLRVPHLLQVVKRRRIKNSLSRKNILYRDN
Sbjct: 121 ICLEFMEKADVLEYYDQTVNSPSGSFYIPAVLRVPHLLQVVKRRRIKNSLSRKNILYRDN 180

Query: 181 YTCQYCSSHESLTIDHVLPISRGGEWTWENLVAACVKCNSKKGQKTVEEANMKLKKTPKA 240
           YTCQYCSSHESLTIDHVLPISRGGEWTWENLVAACVKCNSKKGQKTVEEANMKLKKTPKA
Sbjct: 181 YTCQYCSSHESLTIDHVLPISRGGEWTWENLVAACVKCNSKKGQKTVEEANMKLKKTPKA 240

Query: 241 PKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSSEQ 278
           PKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSSEQ
Sbjct: 241 PKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSSEQ 276

BLAST of CSPI03G40910 vs. NCBI nr
Match: XP_038905692.1 (uncharacterized protein LOC120091663 [Benincasa hispida])

HSP 1 Score: 498.8 bits (1283), Expect = 3.0e-137
Identity = 252/279 (90.32%), Postives = 261/279 (93.55%), Query Frame = 0

Query: 1   MAQFTAHTRLKLLLNGDGLPFGSESKDRFRYKLRSVR--PRRFPLSRPSSSTTSSTSALR 60
           MAQFTAH+R+KLLLNGDG+PFGSE KDRFRYKLR VR   RR PLS PS S TSS SALR
Sbjct: 1   MAQFTAHSRVKLLLNGDGVPFGSEPKDRFRYKLRLVRTLKRRIPLSGPSPS-TSSASALR 60

Query: 61  KPTQHAAEVRVGVRDESVNGGDDDVIGFDYDYELESDDLACFRGLVLDISYRPVNVVCWK 120
           K  QHAAEVRVGVR ESV+ GDD ++  DYDYE E+DDLACFRGLVLDISYRPVNVVCWK
Sbjct: 61  KSAQHAAEVRVGVRGESVS-GDDAIVDLDYDYEFETDDLACFRGLVLDISYRPVNVVCWK 120

Query: 121 RAICLEFMEKADVLEYYDQTVNSPSGSFYIPAVLRVPHLLQVVKRRRIKNSLSRKNILYR 180
           RAICLEFMEKADVLEYYDQTV+SPSGSFYIPAVLRVPHLLQVVKRRRIKNSLSRKNILYR
Sbjct: 121 RAICLEFMEKADVLEYYDQTVSSPSGSFYIPAVLRVPHLLQVVKRRRIKNSLSRKNILYR 180

Query: 181 DNYTCQYCSSHESLTIDHVLPISRGGEWTWENLVAACVKCNSKKGQKTVEEANMKLKKTP 240
           DNYTCQYCSSHESLTIDHVLPISRGGEWTWENLVAACV+CNSKKGQKTVEEANMKLKKTP
Sbjct: 181 DNYTCQYCSSHESLTIDHVLPISRGGEWTWENLVAACVQCNSKKGQKTVEEANMKLKKTP 240

Query: 241 KAPKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSSEQ 278
           KAPKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSSEQ
Sbjct: 241 KAPKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSSEQ 277

BLAST of CSPI03G40910 vs. NCBI nr
Match: XP_022152374.1 (uncharacterized protein LOC111020122 [Momordica charantia])

HSP 1 Score: 484.6 bits (1246), Expect = 5.8e-133
Identity = 246/280 (87.86%), Postives = 255/280 (91.07%), Query Frame = 0

Query: 1   MAQFTAHTRLKLLLNGDGLPFGSESKDRFRYKLRSVRPRRFPLSRPS---SSTTSSTSAL 60
           MAQFT   R+KLLLNGDG+PFGSESKDR RYKLRSV  RR PLS  S   S + SS SAL
Sbjct: 1   MAQFTTLNRVKLLLNGDGVPFGSESKDRLRYKLRSV--RRIPLSAASTGISPSASSASAL 60

Query: 61  RKPTQHAAEVRVGVRDESVNGGDDDVIGFDYDYELESDDLACFRGLVLDISYRPVNVVCW 120
           RK  QH AE+RVGVRDESV+  D  ++G D DYE ESDDLACFRGLVLDISYRPVNVVCW
Sbjct: 61  RKSAQHVAEMRVGVRDESVS-DDGAIVGLDCDYEFESDDLACFRGLVLDISYRPVNVVCW 120

Query: 121 KRAICLEFMEKADVLEYYDQTVNSPSGSFYIPAVLRVPHLLQVVKRRRIKNSLSRKNILY 180
           KRAICLEFMEKADVLEYYDQTVNSPSGSFYIPAVLRVPHLLQVVKRRRIKNSLSRKNILY
Sbjct: 121 KRAICLEFMEKADVLEYYDQTVNSPSGSFYIPAVLRVPHLLQVVKRRRIKNSLSRKNILY 180

Query: 181 RDNYTCQYCSSHESLTIDHVLPISRGGEWTWENLVAACVKCNSKKGQKTVEEANMKLKKT 240
           RDNYTCQYCSSH+SLTIDHVLPISRGGEWTWENLVAACVKCNSKKGQKTVEEANMKLKKT
Sbjct: 181 RDNYTCQYCSSHDSLTIDHVLPISRGGEWTWENLVAACVKCNSKKGQKTVEEANMKLKKT 240

Query: 241 PKAPKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSSEQ 278
           PKAPKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLS+EQ
Sbjct: 241 PKAPKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSNEQ 277

BLAST of CSPI03G40910 vs. NCBI nr
Match: XP_022932702.1 (uncharacterized protein LOC111439170 [Cucurbita moschata] >XP_022932703.1 uncharacterized protein LOC111439170 [Cucurbita moschata] >XP_022972084.1 uncharacterized protein LOC111470725 [Cucurbita maxima] >XP_023539770.1 uncharacterized protein LOC111800353 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 474.6 bits (1220), Expect = 6.0e-130
Identity = 244/283 (86.22%), Postives = 256/283 (90.46%), Query Frame = 0

Query: 1   MAQFTAHTRLKLLLNGDGLPFGSESKDRFRYKLRSVR--PRRFPLSRPSSS----TTSST 60
           MAQFTAH+R+KLLLNGDG+PFGSE KDR R+KLRSVR   RR PLS  SS+    ++SS 
Sbjct: 1   MAQFTAHSRVKLLLNGDGVPFGSEPKDRSRHKLRSVRTLKRRTPLSGASSTGLSPSSSSA 60

Query: 61  SALRKPTQHAAEVRVGVRDESVNGGDDDVIGFDYDYELESDDLACFRGLVLDISYRPVNV 120
           SALRK  Q     RVGVR ESV+ GDD ++  DYDYE ESDDLACFRGLVLDISYRPVNV
Sbjct: 61  SALRKSAQ-----RVGVRGESVS-GDDAILDIDYDYEFESDDLACFRGLVLDISYRPVNV 120

Query: 121 VCWKRAICLEFMEKADVLEYYDQTVNSPSGSFYIPAVLRVPHLLQVVKRRRIKNSLSRKN 180
           VCWKRAICLEFMEKADVLEYYDQTV+SPSGSFYIPAVLRVPHLLQVVKRRRIK SLSRKN
Sbjct: 121 VCWKRAICLEFMEKADVLEYYDQTVSSPSGSFYIPAVLRVPHLLQVVKRRRIKQSLSRKN 180

Query: 181 ILYRDNYTCQYCSSHESLTIDHVLPISRGGEWTWENLVAACVKCNSKKGQKTVEEANMKL 240
           ILYRDNYTCQYCSSHESLTIDHVLP+SRGGEWTWENLVAACVKCNSKKGQKTVEEANMKL
Sbjct: 181 ILYRDNYTCQYCSSHESLTIDHVLPVSRGGEWTWENLVAACVKCNSKKGQKTVEEANMKL 240

Query: 241 KKTPKAPKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSSEQ 278
           KKTPKAPKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSSEQ
Sbjct: 241 KKTPKAPKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSSEQ 277

BLAST of CSPI03G40910 vs. TAIR 10
Match: AT2G23840.1 (HNH endonuclease )

HSP 1 Score: 340.9 bits (873), Expect = 9.7e-94
Identity = 182/284 (64.08%), Postives = 217/284 (76.41%), Query Frame = 0

Query: 1   MAQFTAHTRLKLLLNGDGLPFGSESKDRFRYKL------RSVRPRRFPLSRPSSSTTSST 60
           MA F+A  RLKLL + DGL FG +S+D+FR  L        + P R    +  +   SS 
Sbjct: 1   MAHFSA--RLKLLSSNDGLSFGVDSRDQFRQSLYMAGGGSPLVPIRMSRLKFCARIFSSY 60

Query: 61  SALRKPTQHAAEVRVGVRDESVNGGDDDVIGFDYDYE---LESDD-LACFRGLVLDISYR 120
           S   K      ++R     E     D+D   +D+D +   LE+DD L+CFRGLVLDISYR
Sbjct: 61  SFPYK----RKKIRDLDNTEKNLDIDEDNDEWDFDGDDDGLETDDHLSCFRGLVLDISYR 120

Query: 121 PVNVVCWKRAICLEFMEKADVLEYYDQTVNSPSGSFYIPAVLRVPHLLQVVKRRRIKNSL 180
           PVNVVCWKRAICLE+M+KADVLEYYDQTV+SP+GSFYIPAVLRVPHLLQVVKRRR+KNSL
Sbjct: 121 PVNVVCWKRAICLEYMDKADVLEYYDQTVSSPTGSFYIPAVLRVPHLLQVVKRRRVKNSL 180

Query: 181 SRKNILYRDNYTCQYCSSHESLTIDHVLPISRGGEWTWENLVAACVKCNSKKGQKTVEEA 240
           SRKNIL RD+YTCQYCSS E+LTIDHV+P+SRGGEWTW+NLVAAC +CNS+KGQKT +EA
Sbjct: 181 SRKNILLRDDYTCQYCSSRENLTIDHVMPVSRGGEWTWQNLVAACSRCNSRKGQKTADEA 240

Query: 241 NMKLKKTPKAPKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLS 275
           +MKL K PK PKDYDI+AIPLT+ AI+ML+  KG PEEWRQYL+
Sbjct: 241 HMKLLKVPKEPKDYDIVAIPLTNAAIRMLRSNKGMPEEWRQYLA 278

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A0A0LG991.8e-15699.64HNHc domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G844900 PE=4 S... [more]
A0A5D3E2H25.3e-14895.67HNH endonuclease OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold734G002... [more]
A0A1S3CNK05.3e-14895.67uncharacterized protein LOC103502982 OS=Cucumis melo OX=3656 GN=LOC103502982 PE=... [more]
A0A6J1DFU62.8e-13387.86uncharacterized protein LOC111020122 OS=Momordica charantia OX=3673 GN=LOC111020... [more]
A0A6J1F2H92.9e-13086.22uncharacterized protein LOC111439170 OS=Cucurbita moschata OX=3662 GN=LOC1114391... [more]
Match NameE-valueIdentityDescription
XP_004141149.13.8e-15699.64uncharacterized protein LOC101207660 [Cucumis sativus] >XP_031738556.1 uncharact... [more]
XP_008465340.11.1e-14795.67PREDICTED: uncharacterized protein LOC103502982 [Cucumis melo] >KAA0051262.1 HNH... [more]
XP_038905692.13.0e-13790.32uncharacterized protein LOC120091663 [Benincasa hispida][more]
XP_022152374.15.8e-13387.86uncharacterized protein LOC111020122 [Momordica charantia][more]
XP_022932702.16.0e-13086.22uncharacterized protein LOC111439170 [Cucurbita moschata] >XP_022932703.1 unchar... [more]
Match NameE-valueIdentityDescription
AT2G23840.19.7e-9464.08HNH endonuclease [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003615HNH nucleaseSMARTSM00507HNH_5coord: 170..216
e-value: 1.3E-4
score: 31.4
IPR003615HNH nucleaseCDDcd00085HNHccoord: 172..212
e-value: 4.58824E-7
score: 43.9996
NoneNo IPR availableGENE3D1.10.30.50coord: 158..213
e-value: 3.3E-12
score: 47.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 40..66
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 42..60
NoneNo IPR availablePANTHERPTHR33877:SF2SLL1193 PROTEINcoord: 49..212
NoneNo IPR availablePANTHERPTHR33877SLL1193 PROTEINcoord: 49..212
IPR002711HNH endonucleasePFAMPF01844HNHcoord: 183..212
e-value: 1.4E-6
score: 28.3

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI03G40910.1CSPI03G40910.1mRNA
CSPI03G40910.2CSPI03G40910.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0090305 nucleic acid phosphodiester bond hydrolysis
molecular_function GO:0004519 endonuclease activity
molecular_function GO:0003676 nucleic acid binding