CSPI03G39990 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI03G39990
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Descriptionpolygalacturonase QRT3-like
LocationChr3: 34250099 .. 34251894 (-)
RNA-Seq ExpressionCSPI03G39990
SyntenyCSPI03G39990
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TCGAATTCATCTCATTAATAAGCCGAGTGTGAGAGAGATGAATCCCACAAACTATGCTTACACTTTTAGGCCCTTCCTAGGCTTTGTTTATGCAATTATCATCACTGCATTTGTCACTGTGAGTGAGAGTTCATATTCCACTTCTTATGAAGATCATCACTTCATGAGACGCGTCGACAACTATCGATACAATAGCCTCTCTTCTGCACCTCCTTCTACGGTAACTCTTTATCTCTATTTCCATTGACACAATATATATAAGAGTTGGCATTGATTGGATTGGAATTGTCTAACCTTATGAATGAATGACAAACCATGAAGGCAATGTCCGGATCTCGTGTTTATCACGTGGTTGCCTATGGAGCTGATCCCACTGGAGAATCTGACAGCACTGAGGCTATAGAGAAAGCTATTTTTGATGCATTTGAGTATCCCACAGATGGTCATTTGATGAAAGGCATTTTAGACCTTGGAGGTTCTCAACTTCACCTAGATGGAGGGACTTACAAGATAAGCCGTCCCTTGCGCTTACCGGACATACCTGCTGGCAACTTCATGGTACCTGTTATATAATACTAAGATACATGAATTACTCCTCTTATCATCCATTGGTTTTTGAGGATTGAACTTTTGTCTATTAAGAATGCATTTTACATATGTGGACAGATACATGGAGGATCATTACGCGCGTCCGAATCTTTTCCAAGCAATGGGCATCTTATAGAATTAAGGCCTTCGTCACCTGCTGTGATGTATGAATATATCACACTCAAGGATCTAATGTTAAACTGCAATTTCAGAGGAGGTGGGATAGCCATTATCAACTCATTGAGAACTACAGTAGATAACTGTTACATATCACATTTCATGAGCGAGGGAATAGTAATTGAAGGCGGGCATGAAAGCTACATACGAAACTCCTTCATCGGGCAACACATAAACGTCGGCGGAGACCGAGGCGAAAAGGATTTCTCAGGGATTGGAGTGAAAATAATGGGAAATGACAATTTGTTGAGAGACATAGTGATATATTCAGCAGAGATTGGAGTGATGGTGTTGGGACAAGCAAATGTGATGATGGGAGTACATTGTTACAACAAGGCGAGGGCATTGGGTGGGATAGGAATTTATGTGAAGGAACCTGGTTTTACACAAACAAGGATAGTGAATTGCTACTTGGATTACACAGGAGTGGTGGTGGAGGATCCAGTTCAAGTGCATATAACAGGATGTTTCTTCTTAGGGAATGCATTGGTTGTATTGAAATCGATAGGGGGAGTAATAAGCGGATTGAATATCGTCGATAACATGTTCTCCGGAGATTATACAGGGGTGAGAATAGTTGAGTTAGATGAATCCATGACTCCCTTCACTAGAATTGATCAAGTGGTGGTAGATAGAAATAATGTCCGTGGGATGGTGGTTAAATCGACAGTGGGAAGAGGATCGACGAGGGCGAATGGAACGACTTGGACAGTTGATTTCTCAAGCCTATTGTTGTTTCCTAACCTCATAAAAAATGTAGTGTATTCATTGGAGATGGAGCAGAGTCAGGGTCAAGTATTTCCAAACCATGTTTTAACTAATCTAACTCACAACAGAGTAACTGTGAGGTCCAATTTGGCAATAGCAGCTACATTACATGTTCAAGTACACCAAACAACCTATGCCTTATGATACATACACTATTGTTCATTAGCTTGCTTTCCTCACATTAAAATTGAACTGAAAGATTAATTCATTGTTTGTTGCTTCATCCTTTGATGTATAGACACCAGTAAAGTAAAAGAAACACC

mRNA sequence

TCGAATTCATCTCATTAATAAGCCGAGTGTGAGAGAGATGAATCCCACAAACTATGCTTACACTTTTAGGCCCTTCCTAGGCTTTGTTTATGCAATTATCATCACTGCATTTGTCACTGTGAGTGAGAGTTCATATTCCACTTCTTATGAAGATCATCACTTCATGAGACGCGTCGACAACTATCGATACAATAGCCTCTCTTCTGCACCTCCTTCTACGGCAATGTCCGGATCTCGTGTTTATCACGTGGTTGCCTATGGAGCTGATCCCACTGGAGAATCTGACAGCACTGAGGCTATAGAGAAAGCTATTTTTGATGCATTTGAGTATCCCACAGATGGTCATTTGATGAAAGGCATTTTAGACCTTGGAGGTTCTCAACTTCACCTAGATGGAGGGACTTACAAGATAAGCCGTCCCTTGCGCTTACCGGACATACCTGCTGGCAACTTCATGATACATGGAGGATCATTACGCGCGTCCGAATCTTTTCCAAGCAATGGGCATCTTATAGAATTAAGGCCTTCGTCACCTGCTGTGATGTATGAATATATCACACTCAAGGATCTAATGTTAAACTGCAATTTCAGAGGAGGTGGGATAGCCATTATCAACTCATTGAGAACTACAGTAGATAACTGTTACATATCACATTTCATGAGCGAGGGAATAGTAATTGAAGGCGGGCATGAAAGCTACATACGAAACTCCTTCATCGGGCAACACATAAACGTCGGCGGAGACCGAGGCGAAAAGGATTTCTCAGGGATTGGAGTGAAAATAATGGGAAATGACAATTTGTTGAGAGACATAGTGATATATTCAGCAGAGATTGGAGTGATGGTGTTGGGACAAGCAAATGTGATGATGGGAGTACATTGTTACAACAAGGCGAGGGCATTGGGTGGGATAGGAATTTATGTGAAGGAACCTGGTTTTACACAAACAAGGATAGTGAATTGCTACTTGGATTACACAGGAGTGGTGGTGGAGGATCCAGTTCAAGTGCATATAACAGGATGTTTCTTCTTAGGGAATGCATTGGTTGTATTGAAATCGATAGGGGGAGTAATAAGCGGATTGAATATCGTCGATAACATGTTCTCCGGAGATTATACAGGGGTGAGAATAGTTGAGTTAGATGAATCCATGACTCCCTTCACTAGAATTGATCAAGTGGTGGTAGATAGAAATAATGTCCGTGGGATGGTGGTTAAATCGACAGTGGGAAGAGGATCGACGAGGGCGAATGGAACGACTTGGACAGTTGATTTCTCAAGCCTATTGTTGTTTCCTAACCTCATAAAAAATGTAGTGTATTCATTGGAGATGGAGCAGAGTCAGGGTCAAGTATTTCCAAACCATGTTTTAACTAATCTAACTCACAACAGAGTAACTGTGAGGTCCAATTTGGCAATAGCAGCTACATTACATGTTCAAGTACACCAAACAACCTATGCCTTATGATACATACACTATTGTTCATTAGCTTGCTTTCCTCACATTAAAATTGAACTGAAAGATTAATTCATTGTTTGTTGCTTCATCCTTTGATGTATAGACACCAGTAAAGTAAAAGAAACACC

Coding sequence (CDS)

ATGAATCCCACAAACTATGCTTACACTTTTAGGCCCTTCCTAGGCTTTGTTTATGCAATTATCATCACTGCATTTGTCACTGTGAGTGAGAGTTCATATTCCACTTCTTATGAAGATCATCACTTCATGAGACGCGTCGACAACTATCGATACAATAGCCTCTCTTCTGCACCTCCTTCTACGGCAATGTCCGGATCTCGTGTTTATCACGTGGTTGCCTATGGAGCTGATCCCACTGGAGAATCTGACAGCACTGAGGCTATAGAGAAAGCTATTTTTGATGCATTTGAGTATCCCACAGATGGTCATTTGATGAAAGGCATTTTAGACCTTGGAGGTTCTCAACTTCACCTAGATGGAGGGACTTACAAGATAAGCCGTCCCTTGCGCTTACCGGACATACCTGCTGGCAACTTCATGATACATGGAGGATCATTACGCGCGTCCGAATCTTTTCCAAGCAATGGGCATCTTATAGAATTAAGGCCTTCGTCACCTGCTGTGATGTATGAATATATCACACTCAAGGATCTAATGTTAAACTGCAATTTCAGAGGAGGTGGGATAGCCATTATCAACTCATTGAGAACTACAGTAGATAACTGTTACATATCACATTTCATGAGCGAGGGAATAGTAATTGAAGGCGGGCATGAAAGCTACATACGAAACTCCTTCATCGGGCAACACATAAACGTCGGCGGAGACCGAGGCGAAAAGGATTTCTCAGGGATTGGAGTGAAAATAATGGGAAATGACAATTTGTTGAGAGACATAGTGATATATTCAGCAGAGATTGGAGTGATGGTGTTGGGACAAGCAAATGTGATGATGGGAGTACATTGTTACAACAAGGCGAGGGCATTGGGTGGGATAGGAATTTATGTGAAGGAACCTGGTTTTACACAAACAAGGATAGTGAATTGCTACTTGGATTACACAGGAGTGGTGGTGGAGGATCCAGTTCAAGTGCATATAACAGGATGTTTCTTCTTAGGGAATGCATTGGTTGTATTGAAATCGATAGGGGGAGTAATAAGCGGATTGAATATCGTCGATAACATGTTCTCCGGAGATTATACAGGGGTGAGAATAGTTGAGTTAGATGAATCCATGACTCCCTTCACTAGAATTGATCAAGTGGTGGTAGATAGAAATAATGTCCGTGGGATGGTGGTTAAATCGACAGTGGGAAGAGGATCGACGAGGGCGAATGGAACGACTTGGACAGTTGATTTCTCAAGCCTATTGTTGTTTCCTAACCTCATAAAAAATGTAGTGTATTCATTGGAGATGGAGCAGAGTCAGGGTCAAGTATTTCCAAACCATGTTTTAACTAATCTAACTCACAACAGAGTAACTGTGAGGTCCAATTTGGCAATAGCAGCTACATTACATGTTCAAGTACACCAAACAACCTATGCCTTATGA

Protein sequence

MNPTNYAYTFRPFLGFVYAIIITAFVTVSESSYSTSYEDHHFMRRVDNYRYNSLSSAPPSTAMSGSRVYHVVAYGADPTGESDSTEAIEKAIFDAFEYPTDGHLMKGILDLGGSQLHLDGGTYKISRPLRLPDIPAGNFMIHGGSLRASESFPSNGHLIELRPSSPAVMYEYITLKDLMLNCNFRGGGIAIINSLRTTVDNCYISHFMSEGIVIEGGHESYIRNSFIGQHINVGGDRGEKDFSGIGVKIMGNDNLLRDIVIYSAEIGVMVLGQANVMMGVHCYNKARALGGIGIYVKEPGFTQTRIVNCYLDYTGVVVEDPVQVHITGCFFLGNALVVLKSIGGVISGLNIVDNMFSGDYTGVRIVELDESMTPFTRIDQVVVDRNNVRGMVVKSTVGRGSTRANGTTWTVDFSSLLLFPNLIKNVVYSLEMEQSQGQVFPNHVLTNLTHNRVTVRSNLAIAATLHVQVHQTTYAL*
Homology
BLAST of CSPI03G39990 vs. ExPASy Swiss-Prot
Match: O49432 (Polygalacturonase QRT3 OS=Arabidopsis thaliana OX=3702 GN=QRT3 PE=2 SV=1)

HSP 1 Score: 494.6 bits (1272), Expect = 1.3e-138
Identity = 248/424 (58.49%), Postives = 320/424 (75.47%), Query Frame = 0

Query: 53  SLSSAPPS--TAMSGSRVYHVVAYGADPTGESDSTEAIEKAIFDAFEYPTDGHLMKGILD 112
           +L S PP+   A+ G RVY V++YGADPTG+ DST+AI KA+ +AF+ P  G LM+GI D
Sbjct: 58  ALVSPPPTPPQAVPGPRVYQVISYGADPTGKLDSTDAILKAMEEAFDGPNHGVLMQGIND 117

Query: 113 LGGSQLHLDGGTYKISRPLRLPDIPAGNFMIHGGSLRASESFPSNGHLIELRPSSPAVMY 172
           LGG+++ L GG+Y ISRPLR P   AGN +I GG+LRAS  FP + +LIEL+  S  + Y
Sbjct: 118 LGGARIDLQGGSYLISRPLRFPSAGAGNLLISGGTLRASNDFPVDRYLIELKDESSKLQY 177

Query: 173 --EYITLKDLMLNCNFRGGGIAIINSLRTTVDNCYISHF-MSEGIVIEGGHESYIRNSFI 232
             EYITL+DL+++CN+RGG IA+INSLRT++DNCYI+ F  + GI+++ GHE+YIRNSF+
Sbjct: 178 IFEYITLRDLLIDCNYRGGAIAVINSLRTSIDNCYITRFGDTNGILVKSGHETYIRNSFL 237

Query: 233 GQHINVGGDRGEKDFSGIGVKIMGNDNLLRDIVIYSAEIGVMVLGQANVMMGVHCYNKAR 292
           GQHI  GGDRGE+ FSG  + +MGNDN + D VI+SA IGVMV GQAN++ GVHCYNKA 
Sbjct: 238 GQHITAGGDRGERSFSGTAINLMGNDNAVTDTVIFSARIGVMVSGQANLLSGVHCYNKAT 297

Query: 293 ALGGIGIYVKEPGFTQTRIVNCYLDYTGVVVEDPVQVHITGCFFLGNALVVLKSIGGVIS 352
             GG GIY++ PG TQ RIVN YLDYTG+V EDPVQ+ I+G FFLG+A ++LKSI G I 
Sbjct: 298 GFGGTGIYLRLPGLTQNRIVNSYLDYTGIVAEDPVQLQISGTFFLGDAFILLKSIAGYIR 357

Query: 353 GLNIVDNMFSGDYTGVRIVELDESMTPFTRIDQVVVDRNNVRGMVVKSTVGRGSTRANGT 412
           G++IVDNMFSG   GV+IV+LD+  T F  + QVVVDRN+V GMV KSTV RGS   NGT
Sbjct: 358 GVSIVDNMFSGSGHGVQIVQLDQRNTAFDDVGQVVVDRNSVNGMVEKSTVARGSVDGNGT 417

Query: 413 TWTVDFSSLLLFPNLIKNVVYSLEMEQSQGQVFPNHVLTNLTHNRVTVRSNLAIAATLHV 472
           +WTVDF+ +LLFP+LI +V Y+L    S+  VFP H L N++ NRV V +N  +  T++V
Sbjct: 418 SWTVDFNPVLLFPDLINHVQYTL--VASEAGVFPLHALRNVSDNRVVVETNAPVTGTVYV 477

BLAST of CSPI03G39990 vs. ExPASy TrEMBL
Match: A0A0A0LD06 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G837050 PE=4 SV=1)

HSP 1 Score: 952.2 bits (2460), Expect = 8.2e-274
Identity = 476/476 (100.00%), Postives = 476/476 (100.00%), Query Frame = 0

Query: 1   MNPTNYAYTFRPFLGFVYAIIITAFVTVSESSYSTSYEDHHFMRRVDNYRYNSLSSAPPS 60
           MNPTNYAYTFRPFLGFVYAIIITAFVTVSESSYSTSYEDHHFMRRVDNYRYNSLSSAPPS
Sbjct: 1   MNPTNYAYTFRPFLGFVYAIIITAFVTVSESSYSTSYEDHHFMRRVDNYRYNSLSSAPPS 60

Query: 61  TAMSGSRVYHVVAYGADPTGESDSTEAIEKAIFDAFEYPTDGHLMKGILDLGGSQLHLDG 120
           TAMSGSRVYHVVAYGADPTGESDSTEAIEKAIFDAFEYPTDGHLMKGILDLGGSQLHLDG
Sbjct: 61  TAMSGSRVYHVVAYGADPTGESDSTEAIEKAIFDAFEYPTDGHLMKGILDLGGSQLHLDG 120

Query: 121 GTYKISRPLRLPDIPAGNFMIHGGSLRASESFPSNGHLIELRPSSPAVMYEYITLKDLML 180
           GTYKISRPLRLPDIPAGNFMIHGGSLRASESFPSNGHLIELRPSSPAVMYEYITLKDLML
Sbjct: 121 GTYKISRPLRLPDIPAGNFMIHGGSLRASESFPSNGHLIELRPSSPAVMYEYITLKDLML 180

Query: 181 NCNFRGGGIAIINSLRTTVDNCYISHFMSEGIVIEGGHESYIRNSFIGQHINVGGDRGEK 240
           NCNFRGGGIAIINSLRTTVDNCYISHFMSEGIVIEGGHESYIRNSFIGQHINVGGDRGEK
Sbjct: 181 NCNFRGGGIAIINSLRTTVDNCYISHFMSEGIVIEGGHESYIRNSFIGQHINVGGDRGEK 240

Query: 241 DFSGIGVKIMGNDNLLRDIVIYSAEIGVMVLGQANVMMGVHCYNKARALGGIGIYVKEPG 300
           DFSGIGVKIMGNDNLLRDIVIYSAEIGVMVLGQANVMMGVHCYNKARALGGIGIYVKEPG
Sbjct: 241 DFSGIGVKIMGNDNLLRDIVIYSAEIGVMVLGQANVMMGVHCYNKARALGGIGIYVKEPG 300

Query: 301 FTQTRIVNCYLDYTGVVVEDPVQVHITGCFFLGNALVVLKSIGGVISGLNIVDNMFSGDY 360
           FTQTRIVNCYLDYTGVVVEDPVQVHITGCFFLGNALVVLKSIGGVISGLNIVDNMFSGDY
Sbjct: 301 FTQTRIVNCYLDYTGVVVEDPVQVHITGCFFLGNALVVLKSIGGVISGLNIVDNMFSGDY 360

Query: 361 TGVRIVELDESMTPFTRIDQVVVDRNNVRGMVVKSTVGRGSTRANGTTWTVDFSSLLLFP 420
           TGVRIVELDESMTPFTRIDQVVVDRNNVRGMVVKSTVGRGSTRANGTTWTVDFSSLLLFP
Sbjct: 361 TGVRIVELDESMTPFTRIDQVVVDRNNVRGMVVKSTVGRGSTRANGTTWTVDFSSLLLFP 420

Query: 421 NLIKNVVYSLEMEQSQGQVFPNHVLTNLTHNRVTVRSNLAIAATLHVQVHQTTYAL 477
           NLIKNVVYSLEMEQSQGQVFPNHVLTNLTHNRVTVRSNLAIAATLHVQVHQTTYAL
Sbjct: 421 NLIKNVVYSLEMEQSQGQVFPNHVLTNLTHNRVTVRSNLAIAATLHVQVHQTTYAL 476

BLAST of CSPI03G39990 vs. ExPASy TrEMBL
Match: A0A5D3BRF5 (Polygalacturonase QRT3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1545G00390 PE=4 SV=1)

HSP 1 Score: 893.6 bits (2308), Expect = 3.5e-256
Identity = 456/486 (93.83%), Postives = 464/486 (95.47%), Query Frame = 0

Query: 1   MNPTNYA-YTFRPFL-GFVYAIIITAFVTVSESSYSTSYEDHHFMRRVDNYRYNSLSSA- 60
           MNPTNYA YT  PFL  FV+AIII+ FVTVSESSYSTSYEDHHFMRRVDNYRYN+LSSA 
Sbjct: 1   MNPTNYASYTLMPFLICFVHAIIISTFVTVSESSYSTSYEDHHFMRRVDNYRYNTLSSAA 60

Query: 61  ---PPSTAMSGSRVYHVVAYGADPTGESDSTEAIEKAIFDAFEYPTDGHLMKGILDLGGS 120
              PP TAMSGSRVYHVVAYGADPTGESDSTEAIEKAI DAFEYPTDGHLMKGILDLGGS
Sbjct: 61  PSRPPLTAMSGSRVYHVVAYGADPTGESDSTEAIEKAISDAFEYPTDGHLMKGILDLGGS 120

Query: 121 QLHLDGGTYKISRPLRLPDIPAGNFMIHGGSLRASESFPSNGHLIELRPSSPAVMYEYIT 180
           QLHLDGGTYKISRPLRLPD  AGNFMIHGGSLRASESFPSNGHLIELRPSSP+VMYEYIT
Sbjct: 121 QLHLDGGTYKISRPLRLPDTSAGNFMIHGGSLRASESFPSNGHLIELRPSSPSVMYEYIT 180

Query: 181 LKDLMLNCNFRGGGIAIINSLRTTVDNCYISHFMSEGIVIEGGHESYIRNSFIGQHINVG 240
           LKDLMLNCNFRGGGIAIINSLRTTVDNCY+SHF SEGIVIEGGHESYIRNSFIGQHINVG
Sbjct: 181 LKDLMLNCNFRGGGIAIINSLRTTVDNCYVSHFRSEGIVIEGGHESYIRNSFIGQHINVG 240

Query: 241 GDRGEKDFSGIGVKIMGNDNLLRDIVIYSAEIGVMVLGQANVMMGVHCYNKARALGGIGI 300
           GDRGEKDFSGIGVKIMGNDNLLRDIVIYSAEIGVMVLGQANV+MGVHCYNKARALGG+GI
Sbjct: 241 GDRGEKDFSGIGVKIMGNDNLLRDIVIYSAEIGVMVLGQANVIMGVHCYNKARALGGVGI 300

Query: 301 YVKEPGFTQTRIVNCYLDYTGVVVEDPVQVHITGCFFLGNALVVLKSIGGVISGLNIVDN 360
           YVKEPGFTQTRIVNCYLDYTGVV EDPVQVHITGCFFLGNALVVLKSIGGVISGLNIVDN
Sbjct: 301 YVKEPGFTQTRIVNCYLDYTGVVAEDPVQVHITGCFFLGNALVVLKSIGGVISGLNIVDN 360

Query: 361 MFSGDYTGVRIVELDESMTPFTRIDQVVVDRNNVRGMVVKSTVGRGSTRANGTTWTVDFS 420
           MFSGDYTGVRIVELDES TPFTRIDQVVVDRNNVRGMVVKSTVGRGSTRANGTTWTVDFS
Sbjct: 361 MFSGDYTGVRIVELDESKTPFTRIDQVVVDRNNVRGMVVKSTVGRGSTRANGTTWTVDFS 420

Query: 421 SLLLFPNLIKNVVYSLEMEQSQG----QVFPNHVLTNLTHNRVTVRSNLAIAATLHVQVH 477
           SLLLFPNLIKNVVYSLEMEQS+G    QV PNHVLTNLTHNRVTVRSNLAIAATLHVQVH
Sbjct: 421 SLLLFPNLIKNVVYSLEMEQSEGQGQIQVLPNHVLTNLTHNRVTVRSNLAIAATLHVQVH 480

BLAST of CSPI03G39990 vs. ExPASy TrEMBL
Match: A0A1S3B7S6 (polygalacturonase QRT3-like OS=Cucumis melo OX=3656 GN=LOC103487092 PE=4 SV=1)

HSP 1 Score: 893.6 bits (2308), Expect = 3.5e-256
Identity = 456/486 (93.83%), Postives = 464/486 (95.47%), Query Frame = 0

Query: 1   MNPTNYA-YTFRPFL-GFVYAIIITAFVTVSESSYSTSYEDHHFMRRVDNYRYNSLSSA- 60
           MNPTNYA YT  PFL  FV+AIII+ FVTVSESSYSTSYEDHHFMRRVDNYRYN+LSSA 
Sbjct: 1   MNPTNYASYTLMPFLICFVHAIIISTFVTVSESSYSTSYEDHHFMRRVDNYRYNTLSSAA 60

Query: 61  ---PPSTAMSGSRVYHVVAYGADPTGESDSTEAIEKAIFDAFEYPTDGHLMKGILDLGGS 120
              PP TAMSGSRVYHVVAYGADPTGESDSTEAIEKAI DAFEYPTDGHLMKGILDLGGS
Sbjct: 61  PSRPPLTAMSGSRVYHVVAYGADPTGESDSTEAIEKAISDAFEYPTDGHLMKGILDLGGS 120

Query: 121 QLHLDGGTYKISRPLRLPDIPAGNFMIHGGSLRASESFPSNGHLIELRPSSPAVMYEYIT 180
           QLHLDGGTYKISRPLRLPD  AGNFMIHGGSLRASESFPSNGHLIELRPSSP+VMYEYIT
Sbjct: 121 QLHLDGGTYKISRPLRLPDTSAGNFMIHGGSLRASESFPSNGHLIELRPSSPSVMYEYIT 180

Query: 181 LKDLMLNCNFRGGGIAIINSLRTTVDNCYISHFMSEGIVIEGGHESYIRNSFIGQHINVG 240
           LKDLMLNCNFRGGGIAIINSLRTTVDNCY+SHF SEGIVIEGGHESYIRNSFIGQHINVG
Sbjct: 181 LKDLMLNCNFRGGGIAIINSLRTTVDNCYVSHFRSEGIVIEGGHESYIRNSFIGQHINVG 240

Query: 241 GDRGEKDFSGIGVKIMGNDNLLRDIVIYSAEIGVMVLGQANVMMGVHCYNKARALGGIGI 300
           GDRGEKDFSGIGVKIMGNDNLLRDIVIYSAEIGVMVLGQANV+MGVHCYNKARALGG+GI
Sbjct: 241 GDRGEKDFSGIGVKIMGNDNLLRDIVIYSAEIGVMVLGQANVIMGVHCYNKARALGGVGI 300

Query: 301 YVKEPGFTQTRIVNCYLDYTGVVVEDPVQVHITGCFFLGNALVVLKSIGGVISGLNIVDN 360
           YVKEPGFTQTRIVNCYLDYTGVV EDPVQVHITGCFFLGNALVVLKSIGGVISGLNIVDN
Sbjct: 301 YVKEPGFTQTRIVNCYLDYTGVVAEDPVQVHITGCFFLGNALVVLKSIGGVISGLNIVDN 360

Query: 361 MFSGDYTGVRIVELDESMTPFTRIDQVVVDRNNVRGMVVKSTVGRGSTRANGTTWTVDFS 420
           MFSGDYTGVRIVELDES TPFTRIDQVVVDRNNVRGMVVKSTVGRGSTRANGTTWTVDFS
Sbjct: 361 MFSGDYTGVRIVELDESKTPFTRIDQVVVDRNNVRGMVVKSTVGRGSTRANGTTWTVDFS 420

Query: 421 SLLLFPNLIKNVVYSLEMEQSQG----QVFPNHVLTNLTHNRVTVRSNLAIAATLHVQVH 477
           SLLLFPNLIKNVVYSLEMEQS+G    QV PNHVLTNLTHNRVTVRSNLAIAATLHVQVH
Sbjct: 421 SLLLFPNLIKNVVYSLEMEQSEGQGQIQVLPNHVLTNLTHNRVTVRSNLAIAATLHVQVH 480

BLAST of CSPI03G39990 vs. ExPASy TrEMBL
Match: A0A6J1J1V1 (polygalacturonase QRT3-like OS=Cucurbita maxima OX=3661 GN=LOC111481950 PE=4 SV=1)

HSP 1 Score: 833.9 bits (2153), Expect = 3.3e-238
Identity = 423/496 (85.28%), Postives = 452/496 (91.13%), Query Frame = 0

Query: 1   MNPTNYAYTFRPFLGFVYAIIITAFVTVSESSYSTSYEDHHFMRRVD-----NYRYNSLS 60
           MNP +YA T  PF+ FVYA+ I+ FV VSESSYSTSYED HFMRR+D     NY+Y+ LS
Sbjct: 1   MNPRSYASTSWPFIAFVYAVTISEFVIVSESSYSTSYED-HFMRRLDPSRLHNYQYDGLS 60

Query: 61  SAPPS---------TAMSGSRVYHVVAYGADPTGESDSTEAIEKAIFDAFEYPTDGHLMK 120
           SA PS         T +SGSRVYHVV+YGADPTGE+DSTEAIEKAI DAF YPTD HLMK
Sbjct: 61  SASPSHTYPSQSHLTPISGSRVYHVVSYGADPTGETDSTEAIEKAISDAFRYPTDAHLMK 120

Query: 121 GILDLGGSQLHLDGGTYKISRPLRLPDIPAGNFMIHGGSLRASESFPSNGHLIELRPSSP 180
           GILDLGGSQ+HLDGGTYKISRPLRLPDI AGNFMIHGGSLRAS+SFPS+GHLIELRPSSP
Sbjct: 121 GILDLGGSQIHLDGGTYKISRPLRLPDIAAGNFMIHGGSLRASDSFPSDGHLIELRPSSP 180

Query: 181 AVMYEYITLKDLMLNCNFRGGGIAIINSLRTTVDNCYISHFMSEGIVIEGGHESYIRNSF 240
           +VMYEYITLKDLMLNCNFRGGGIAI+NS+RTTVDNCYISHFMSEGIVIEGGHESYIRNSF
Sbjct: 181 SVMYEYITLKDLMLNCNFRGGGIAIVNSVRTTVDNCYISHFMSEGIVIEGGHESYIRNSF 240

Query: 241 IGQHINVGGDRGEKDFSGIGVKIMGNDNLLRDIVIYSAEIGVMVLGQANVMMGVHCYNKA 300
           IGQHIN+GGDRGEKDFSGIGVK+MGNDN+LRDIVIYSAEIGVM+LGQANV+MGVHCYNKA
Sbjct: 241 IGQHINMGGDRGEKDFSGIGVKMMGNDNVLRDIVIYSAEIGVMLLGQANVLMGVHCYNKA 300

Query: 301 RALGGIGIYVKEPGFTQTRIVNCYLDYTGVVVEDPVQVHITGCFFLGNALVVLKSIGGVI 360
           R LGGIGIYV  PGFTQTRIVNCYLDYTGVVVEDPVQVHITGCFFLGNALVVLKSIGGVI
Sbjct: 301 RGLGGIGIYVGSPGFTQTRIVNCYLDYTGVVVEDPVQVHITGCFFLGNALVVLKSIGGVI 360

Query: 361 SGLNIVDNMFSGDYTGVRIVELDESMTPFTRIDQVVVDRNNVRGMVVKSTVGRGSTRANG 420
           SGLNIVDNMFSGDYTGV+IVELDES TPFTRIDQVVVDRNNVRGMVVK+TVGRGSTRANG
Sbjct: 361 SGLNIVDNMFSGDYTGVKIVELDESKTPFTRIDQVVVDRNNVRGMVVKTTVGRGSTRANG 420

Query: 421 TTWTVDFSSLLLFPNLIKNVVYSLEMEQSQG------QVFPNHVLTNLTHNRVTVRSNLA 477
           TTWT DFSSLLLF NLIKNV YSLEMEQ+QG      QVFPNH LTN++HNRVTV+SN+A
Sbjct: 421 TTWTFDFSSLLLFRNLIKNVGYSLEMEQTQGQGQGQVQVFPNHALTNVSHNRVTVQSNVA 480

BLAST of CSPI03G39990 vs. ExPASy TrEMBL
Match: A0A6J1F315 (polygalacturonase QRT3-like OS=Cucurbita moschata OX=3662 GN=LOC111441905 PE=4 SV=1)

HSP 1 Score: 833.9 bits (2153), Expect = 3.3e-238
Identity = 426/497 (85.71%), Postives = 450/497 (90.54%), Query Frame = 0

Query: 1   MNPTNYAYTFRPFLGFVYAIIITAFVTVSESSYSTSYEDHHFMRRVD-----NYRYNSLS 60
           MNP +YA T  PF+ FVYA+ I+ FV VSESSYSTSYED  FMRR+D     NY+YN LS
Sbjct: 1   MNPRSYASTSWPFIAFVYAVTISEFVIVSESSYSTSYED-RFMRRLDPSRLHNYQYNGLS 60

Query: 61  SAPPS---------TAMSGSRVYHVVAYGADPTGESDSTEAIEKAIFDAFEYPTDGHLMK 120
           SA PS         T +SGSRVYHVVAYGADPTGESDSTEAIEKAI DAF YPTD HLMK
Sbjct: 61  SASPSHTYPSQSHLTPLSGSRVYHVVAYGADPTGESDSTEAIEKAISDAFRYPTDAHLMK 120

Query: 121 GILDLGGSQLHLDGGTYKISRPLRLPDIPAGNFMIHGGSLRASESFPSNGHLIELRPSSP 180
           GILDLGGSQ+HLDGGTYKISRPLRLPDI AGNFMIHGGSLRAS+SFPS+GHLIELR SSP
Sbjct: 121 GILDLGGSQIHLDGGTYKISRPLRLPDIAAGNFMIHGGSLRASDSFPSDGHLIELRSSSP 180

Query: 181 AVMYEYITLKDLMLNCNFRGGGIAIINSLRTTVDNCYISHFMSEGIVIEGGHESYIRNSF 240
           +VMYEYITLKDLMLNCNFRGGGIAI+NSLRTTVDNCYISHFMSEGIVIEGGHESYIRNSF
Sbjct: 181 SVMYEYITLKDLMLNCNFRGGGIAIVNSLRTTVDNCYISHFMSEGIVIEGGHESYIRNSF 240

Query: 241 IGQHINVGGDRGEKDFSGIGVKIMGNDNLLRDIVIYSAEIGVMVLGQANVMMGVHCYNKA 300
           IGQHIN+GGDRGEKDFSGIGVK++GNDN+LRDIVIYSAEIGVMVLGQANV+MGVHCYNKA
Sbjct: 241 IGQHINMGGDRGEKDFSGIGVKMIGNDNVLRDIVIYSAEIGVMVLGQANVLMGVHCYNKA 300

Query: 301 RALGGIGIYVKEPGFTQTRIVNCYLDYTGVVVEDPVQVHITGCFFLGNALVVLKSIGGVI 360
           R LGGIGIYV  PGFTQTRIVNCYLDYTGVVVEDPVQVHITGCFFLGNALVVLKSIGGVI
Sbjct: 301 RGLGGIGIYVGSPGFTQTRIVNCYLDYTGVVVEDPVQVHITGCFFLGNALVVLKSIGGVI 360

Query: 361 SGLNIVDNMFSGDYTGVRIVELDESMTPFTRIDQVVVDRNNVRGMVVKSTVGRGSTRANG 420
           SGLNIVDNMFSGDYTGVRIVELDES TPFTRIDQVVVDRNNVRGMVVK+TVGRGSTRANG
Sbjct: 361 SGLNIVDNMFSGDYTGVRIVELDESKTPFTRIDQVVVDRNNVRGMVVKTTVGRGSTRANG 420

Query: 421 TTWTVDFSSLLLFPNLIKNVVYSLEMEQSQG--------QVFPNHVLTNLTHNRVTVRSN 476
           TTWTVDFSSLLLF NLIKNV YSLEMEQ+QG        QVFPNH LTN++HNRVTV+SN
Sbjct: 421 TTWTVDFSSLLLFRNLIKNVGYSLEMEQTQGQGQGQGQVQVFPNHALTNVSHNRVTVQSN 480

BLAST of CSPI03G39990 vs. NCBI nr
Match: XP_004141189.2 (polygalacturonase QRT3 [Cucumis sativus] >KGN59673.1 hypothetical protein Csa_001831 [Cucumis sativus])

HSP 1 Score: 952.2 bits (2460), Expect = 1.7e-273
Identity = 476/476 (100.00%), Postives = 476/476 (100.00%), Query Frame = 0

Query: 1   MNPTNYAYTFRPFLGFVYAIIITAFVTVSESSYSTSYEDHHFMRRVDNYRYNSLSSAPPS 60
           MNPTNYAYTFRPFLGFVYAIIITAFVTVSESSYSTSYEDHHFMRRVDNYRYNSLSSAPPS
Sbjct: 1   MNPTNYAYTFRPFLGFVYAIIITAFVTVSESSYSTSYEDHHFMRRVDNYRYNSLSSAPPS 60

Query: 61  TAMSGSRVYHVVAYGADPTGESDSTEAIEKAIFDAFEYPTDGHLMKGILDLGGSQLHLDG 120
           TAMSGSRVYHVVAYGADPTGESDSTEAIEKAIFDAFEYPTDGHLMKGILDLGGSQLHLDG
Sbjct: 61  TAMSGSRVYHVVAYGADPTGESDSTEAIEKAIFDAFEYPTDGHLMKGILDLGGSQLHLDG 120

Query: 121 GTYKISRPLRLPDIPAGNFMIHGGSLRASESFPSNGHLIELRPSSPAVMYEYITLKDLML 180
           GTYKISRPLRLPDIPAGNFMIHGGSLRASESFPSNGHLIELRPSSPAVMYEYITLKDLML
Sbjct: 121 GTYKISRPLRLPDIPAGNFMIHGGSLRASESFPSNGHLIELRPSSPAVMYEYITLKDLML 180

Query: 181 NCNFRGGGIAIINSLRTTVDNCYISHFMSEGIVIEGGHESYIRNSFIGQHINVGGDRGEK 240
           NCNFRGGGIAIINSLRTTVDNCYISHFMSEGIVIEGGHESYIRNSFIGQHINVGGDRGEK
Sbjct: 181 NCNFRGGGIAIINSLRTTVDNCYISHFMSEGIVIEGGHESYIRNSFIGQHINVGGDRGEK 240

Query: 241 DFSGIGVKIMGNDNLLRDIVIYSAEIGVMVLGQANVMMGVHCYNKARALGGIGIYVKEPG 300
           DFSGIGVKIMGNDNLLRDIVIYSAEIGVMVLGQANVMMGVHCYNKARALGGIGIYVKEPG
Sbjct: 241 DFSGIGVKIMGNDNLLRDIVIYSAEIGVMVLGQANVMMGVHCYNKARALGGIGIYVKEPG 300

Query: 301 FTQTRIVNCYLDYTGVVVEDPVQVHITGCFFLGNALVVLKSIGGVISGLNIVDNMFSGDY 360
           FTQTRIVNCYLDYTGVVVEDPVQVHITGCFFLGNALVVLKSIGGVISGLNIVDNMFSGDY
Sbjct: 301 FTQTRIVNCYLDYTGVVVEDPVQVHITGCFFLGNALVVLKSIGGVISGLNIVDNMFSGDY 360

Query: 361 TGVRIVELDESMTPFTRIDQVVVDRNNVRGMVVKSTVGRGSTRANGTTWTVDFSSLLLFP 420
           TGVRIVELDESMTPFTRIDQVVVDRNNVRGMVVKSTVGRGSTRANGTTWTVDFSSLLLFP
Sbjct: 361 TGVRIVELDESMTPFTRIDQVVVDRNNVRGMVVKSTVGRGSTRANGTTWTVDFSSLLLFP 420

Query: 421 NLIKNVVYSLEMEQSQGQVFPNHVLTNLTHNRVTVRSNLAIAATLHVQVHQTTYAL 477
           NLIKNVVYSLEMEQSQGQVFPNHVLTNLTHNRVTVRSNLAIAATLHVQVHQTTYAL
Sbjct: 421 NLIKNVVYSLEMEQSQGQVFPNHVLTNLTHNRVTVRSNLAIAATLHVQVHQTTYAL 476

BLAST of CSPI03G39990 vs. NCBI nr
Match: XP_008443524.2 (PREDICTED: polygalacturonase QRT3-like [Cucumis melo] >KAA0053667.1 polygalacturonase QRT3-like [Cucumis melo var. makuwa] >TYK00836.1 polygalacturonase QRT3-like [Cucumis melo var. makuwa])

HSP 1 Score: 893.6 bits (2308), Expect = 7.2e-256
Identity = 456/486 (93.83%), Postives = 464/486 (95.47%), Query Frame = 0

Query: 1   MNPTNYA-YTFRPFL-GFVYAIIITAFVTVSESSYSTSYEDHHFMRRVDNYRYNSLSSA- 60
           MNPTNYA YT  PFL  FV+AIII+ FVTVSESSYSTSYEDHHFMRRVDNYRYN+LSSA 
Sbjct: 1   MNPTNYASYTLMPFLICFVHAIIISTFVTVSESSYSTSYEDHHFMRRVDNYRYNTLSSAA 60

Query: 61  ---PPSTAMSGSRVYHVVAYGADPTGESDSTEAIEKAIFDAFEYPTDGHLMKGILDLGGS 120
              PP TAMSGSRVYHVVAYGADPTGESDSTEAIEKAI DAFEYPTDGHLMKGILDLGGS
Sbjct: 61  PSRPPLTAMSGSRVYHVVAYGADPTGESDSTEAIEKAISDAFEYPTDGHLMKGILDLGGS 120

Query: 121 QLHLDGGTYKISRPLRLPDIPAGNFMIHGGSLRASESFPSNGHLIELRPSSPAVMYEYIT 180
           QLHLDGGTYKISRPLRLPD  AGNFMIHGGSLRASESFPSNGHLIELRPSSP+VMYEYIT
Sbjct: 121 QLHLDGGTYKISRPLRLPDTSAGNFMIHGGSLRASESFPSNGHLIELRPSSPSVMYEYIT 180

Query: 181 LKDLMLNCNFRGGGIAIINSLRTTVDNCYISHFMSEGIVIEGGHESYIRNSFIGQHINVG 240
           LKDLMLNCNFRGGGIAIINSLRTTVDNCY+SHF SEGIVIEGGHESYIRNSFIGQHINVG
Sbjct: 181 LKDLMLNCNFRGGGIAIINSLRTTVDNCYVSHFRSEGIVIEGGHESYIRNSFIGQHINVG 240

Query: 241 GDRGEKDFSGIGVKIMGNDNLLRDIVIYSAEIGVMVLGQANVMMGVHCYNKARALGGIGI 300
           GDRGEKDFSGIGVKIMGNDNLLRDIVIYSAEIGVMVLGQANV+MGVHCYNKARALGG+GI
Sbjct: 241 GDRGEKDFSGIGVKIMGNDNLLRDIVIYSAEIGVMVLGQANVIMGVHCYNKARALGGVGI 300

Query: 301 YVKEPGFTQTRIVNCYLDYTGVVVEDPVQVHITGCFFLGNALVVLKSIGGVISGLNIVDN 360
           YVKEPGFTQTRIVNCYLDYTGVV EDPVQVHITGCFFLGNALVVLKSIGGVISGLNIVDN
Sbjct: 301 YVKEPGFTQTRIVNCYLDYTGVVAEDPVQVHITGCFFLGNALVVLKSIGGVISGLNIVDN 360

Query: 361 MFSGDYTGVRIVELDESMTPFTRIDQVVVDRNNVRGMVVKSTVGRGSTRANGTTWTVDFS 420
           MFSGDYTGVRIVELDES TPFTRIDQVVVDRNNVRGMVVKSTVGRGSTRANGTTWTVDFS
Sbjct: 361 MFSGDYTGVRIVELDESKTPFTRIDQVVVDRNNVRGMVVKSTVGRGSTRANGTTWTVDFS 420

Query: 421 SLLLFPNLIKNVVYSLEMEQSQG----QVFPNHVLTNLTHNRVTVRSNLAIAATLHVQVH 477
           SLLLFPNLIKNVVYSLEMEQS+G    QV PNHVLTNLTHNRVTVRSNLAIAATLHVQVH
Sbjct: 421 SLLLFPNLIKNVVYSLEMEQSEGQGQIQVLPNHVLTNLTHNRVTVRSNLAIAATLHVQVH 480

BLAST of CSPI03G39990 vs. NCBI nr
Match: XP_038905582.1 (polygalacturonase QRT3-like [Benincasa hispida])

HSP 1 Score: 863.2 bits (2229), Expect = 1.0e-246
Identity = 437/483 (90.48%), Postives = 454/483 (94.00%), Query Frame = 0

Query: 1   MNPTNYAYTFRPFLGFVYAIIITAFVTVSESSYSTSYEDHHFMRRVDNYRYNSLSSAPPS 60
           MNP NYAYTFRPFLGFVYAIII+A V VSESSYSTSYEDHH MRR+DNY Y SLSS  PS
Sbjct: 1   MNPINYAYTFRPFLGFVYAIIISALVIVSESSYSTSYEDHHLMRRIDNYPY-SLSSVAPS 60

Query: 61  ----TAMSGSRVYHVVAYGADPTGESDSTEAIEKAIFDAFEYPTDGHLMKGILDLGGSQL 120
               T  SGSRVYHVVAYGADPTGESDSTEAIEKAI DAF Y TDGHLMKGILDLGGSQL
Sbjct: 61  RSHLTETSGSRVYHVVAYGADPTGESDSTEAIEKAISDAFSYATDGHLMKGILDLGGSQL 120

Query: 121 HLDGGTYKISRPLRLPDIPAGNFMIHGGSLRASESFPSNGHLIELRPSSPAVMYEYITLK 180
           HLDGGTYKISRPLRLPD+ AGNFMIHGGSLRASESFPS+GHLIELRPSSP+VMYEYITLK
Sbjct: 121 HLDGGTYKISRPLRLPDVAAGNFMIHGGSLRASESFPSDGHLIELRPSSPSVMYEYITLK 180

Query: 181 DLMLNCNFRGGGIAIINSLRTTVDNCYISHFMSEGIVIEGGHESYIRNSFIGQHINVGGD 240
           DLMLNCNFRGGGIAIINSLRTTVDNCYISH  SEGIVIEGGHESYIRNSFIGQHINVGGD
Sbjct: 181 DLMLNCNFRGGGIAIINSLRTTVDNCYISHLTSEGIVIEGGHESYIRNSFIGQHINVGGD 240

Query: 241 RGEKDFSGIGVKIMGNDNLLRDIVIYSAEIGVMVLGQANVMMGVHCYNKARALGGIGIYV 300
             EKDFSGIGVK+MGNDN+LRDIVIYSAEIGVMVLGQANV+MGVHCYNKAR LGG+GIYV
Sbjct: 241 GYEKDFSGIGVKVMGNDNVLRDIVIYSAEIGVMVLGQANVIMGVHCYNKARGLGGVGIYV 300

Query: 301 KEPGFTQTRIVNCYLDYTGVVVEDPVQVHITGCFFLGNALVVLKSIGGVISGLNIVDNMF 360
           K+PGFTQTRIVNCYLDYTG+VVEDPVQVHITGCFFLGNALVVLKSIGGVISGLNIVDNMF
Sbjct: 301 KQPGFTQTRIVNCYLDYTGIVVEDPVQVHITGCFFLGNALVVLKSIGGVISGLNIVDNMF 360

Query: 361 SGDYTGVRIVELDESMTPFTRIDQVVVDRNNVRGMVVKSTVGRGSTR-ANGTTWTVDFSS 420
           +GDYTGV+IVELDES TPFTRIDQVVVDRNNVRGMVVK+TVGRGSTR  NGTTWTVDFSS
Sbjct: 361 AGDYTGVKIVELDESKTPFTRIDQVVVDRNNVRGMVVKTTVGRGSTRVTNGTTWTVDFSS 420

Query: 421 LLLFPNLIKNVVYSLEMEQS--QGQVFPNHVLTNLTHNRVTVRSNLAIAATLHVQVHQTT 477
           LLLFPNLI+NVVYSLEMEQS  QGQVFPNHVLTNL HNRVTVRSN+A+AATLHVQVHQTT
Sbjct: 421 LLLFPNLIRNVVYSLEMEQSEGQGQVFPNHVLTNLAHNRVTVRSNVAVAATLHVQVHQTT 480

BLAST of CSPI03G39990 vs. NCBI nr
Match: XP_022983336.1 (polygalacturonase QRT3-like [Cucurbita maxima])

HSP 1 Score: 833.9 bits (2153), Expect = 6.7e-238
Identity = 423/496 (85.28%), Postives = 452/496 (91.13%), Query Frame = 0

Query: 1   MNPTNYAYTFRPFLGFVYAIIITAFVTVSESSYSTSYEDHHFMRRVD-----NYRYNSLS 60
           MNP +YA T  PF+ FVYA+ I+ FV VSESSYSTSYED HFMRR+D     NY+Y+ LS
Sbjct: 1   MNPRSYASTSWPFIAFVYAVTISEFVIVSESSYSTSYED-HFMRRLDPSRLHNYQYDGLS 60

Query: 61  SAPPS---------TAMSGSRVYHVVAYGADPTGESDSTEAIEKAIFDAFEYPTDGHLMK 120
           SA PS         T +SGSRVYHVV+YGADPTGE+DSTEAIEKAI DAF YPTD HLMK
Sbjct: 61  SASPSHTYPSQSHLTPISGSRVYHVVSYGADPTGETDSTEAIEKAISDAFRYPTDAHLMK 120

Query: 121 GILDLGGSQLHLDGGTYKISRPLRLPDIPAGNFMIHGGSLRASESFPSNGHLIELRPSSP 180
           GILDLGGSQ+HLDGGTYKISRPLRLPDI AGNFMIHGGSLRAS+SFPS+GHLIELRPSSP
Sbjct: 121 GILDLGGSQIHLDGGTYKISRPLRLPDIAAGNFMIHGGSLRASDSFPSDGHLIELRPSSP 180

Query: 181 AVMYEYITLKDLMLNCNFRGGGIAIINSLRTTVDNCYISHFMSEGIVIEGGHESYIRNSF 240
           +VMYEYITLKDLMLNCNFRGGGIAI+NS+RTTVDNCYISHFMSEGIVIEGGHESYIRNSF
Sbjct: 181 SVMYEYITLKDLMLNCNFRGGGIAIVNSVRTTVDNCYISHFMSEGIVIEGGHESYIRNSF 240

Query: 241 IGQHINVGGDRGEKDFSGIGVKIMGNDNLLRDIVIYSAEIGVMVLGQANVMMGVHCYNKA 300
           IGQHIN+GGDRGEKDFSGIGVK+MGNDN+LRDIVIYSAEIGVM+LGQANV+MGVHCYNKA
Sbjct: 241 IGQHINMGGDRGEKDFSGIGVKMMGNDNVLRDIVIYSAEIGVMLLGQANVLMGVHCYNKA 300

Query: 301 RALGGIGIYVKEPGFTQTRIVNCYLDYTGVVVEDPVQVHITGCFFLGNALVVLKSIGGVI 360
           R LGGIGIYV  PGFTQTRIVNCYLDYTGVVVEDPVQVHITGCFFLGNALVVLKSIGGVI
Sbjct: 301 RGLGGIGIYVGSPGFTQTRIVNCYLDYTGVVVEDPVQVHITGCFFLGNALVVLKSIGGVI 360

Query: 361 SGLNIVDNMFSGDYTGVRIVELDESMTPFTRIDQVVVDRNNVRGMVVKSTVGRGSTRANG 420
           SGLNIVDNMFSGDYTGV+IVELDES TPFTRIDQVVVDRNNVRGMVVK+TVGRGSTRANG
Sbjct: 361 SGLNIVDNMFSGDYTGVKIVELDESKTPFTRIDQVVVDRNNVRGMVVKTTVGRGSTRANG 420

Query: 421 TTWTVDFSSLLLFPNLIKNVVYSLEMEQSQG------QVFPNHVLTNLTHNRVTVRSNLA 477
           TTWT DFSSLLLF NLIKNV YSLEMEQ+QG      QVFPNH LTN++HNRVTV+SN+A
Sbjct: 421 TTWTFDFSSLLLFRNLIKNVGYSLEMEQTQGQGQGQVQVFPNHALTNVSHNRVTVQSNVA 480

BLAST of CSPI03G39990 vs. NCBI nr
Match: XP_022934866.1 (polygalacturonase QRT3-like [Cucurbita moschata])

HSP 1 Score: 833.9 bits (2153), Expect = 6.7e-238
Identity = 426/497 (85.71%), Postives = 450/497 (90.54%), Query Frame = 0

Query: 1   MNPTNYAYTFRPFLGFVYAIIITAFVTVSESSYSTSYEDHHFMRRVD-----NYRYNSLS 60
           MNP +YA T  PF+ FVYA+ I+ FV VSESSYSTSYED  FMRR+D     NY+YN LS
Sbjct: 1   MNPRSYASTSWPFIAFVYAVTISEFVIVSESSYSTSYED-RFMRRLDPSRLHNYQYNGLS 60

Query: 61  SAPPS---------TAMSGSRVYHVVAYGADPTGESDSTEAIEKAIFDAFEYPTDGHLMK 120
           SA PS         T +SGSRVYHVVAYGADPTGESDSTEAIEKAI DAF YPTD HLMK
Sbjct: 61  SASPSHTYPSQSHLTPLSGSRVYHVVAYGADPTGESDSTEAIEKAISDAFRYPTDAHLMK 120

Query: 121 GILDLGGSQLHLDGGTYKISRPLRLPDIPAGNFMIHGGSLRASESFPSNGHLIELRPSSP 180
           GILDLGGSQ+HLDGGTYKISRPLRLPDI AGNFMIHGGSLRAS+SFPS+GHLIELR SSP
Sbjct: 121 GILDLGGSQIHLDGGTYKISRPLRLPDIAAGNFMIHGGSLRASDSFPSDGHLIELRSSSP 180

Query: 181 AVMYEYITLKDLMLNCNFRGGGIAIINSLRTTVDNCYISHFMSEGIVIEGGHESYIRNSF 240
           +VMYEYITLKDLMLNCNFRGGGIAI+NSLRTTVDNCYISHFMSEGIVIEGGHESYIRNSF
Sbjct: 181 SVMYEYITLKDLMLNCNFRGGGIAIVNSLRTTVDNCYISHFMSEGIVIEGGHESYIRNSF 240

Query: 241 IGQHINVGGDRGEKDFSGIGVKIMGNDNLLRDIVIYSAEIGVMVLGQANVMMGVHCYNKA 300
           IGQHIN+GGDRGEKDFSGIGVK++GNDN+LRDIVIYSAEIGVMVLGQANV+MGVHCYNKA
Sbjct: 241 IGQHINMGGDRGEKDFSGIGVKMIGNDNVLRDIVIYSAEIGVMVLGQANVLMGVHCYNKA 300

Query: 301 RALGGIGIYVKEPGFTQTRIVNCYLDYTGVVVEDPVQVHITGCFFLGNALVVLKSIGGVI 360
           R LGGIGIYV  PGFTQTRIVNCYLDYTGVVVEDPVQVHITGCFFLGNALVVLKSIGGVI
Sbjct: 301 RGLGGIGIYVGSPGFTQTRIVNCYLDYTGVVVEDPVQVHITGCFFLGNALVVLKSIGGVI 360

Query: 361 SGLNIVDNMFSGDYTGVRIVELDESMTPFTRIDQVVVDRNNVRGMVVKSTVGRGSTRANG 420
           SGLNIVDNMFSGDYTGVRIVELDES TPFTRIDQVVVDRNNVRGMVVK+TVGRGSTRANG
Sbjct: 361 SGLNIVDNMFSGDYTGVRIVELDESKTPFTRIDQVVVDRNNVRGMVVKTTVGRGSTRANG 420

Query: 421 TTWTVDFSSLLLFPNLIKNVVYSLEMEQSQG--------QVFPNHVLTNLTHNRVTVRSN 476
           TTWTVDFSSLLLF NLIKNV YSLEMEQ+QG        QVFPNH LTN++HNRVTV+SN
Sbjct: 421 TTWTVDFSSLLLFRNLIKNVGYSLEMEQTQGQGQGQGQVQVFPNHALTNVSHNRVTVQSN 480

BLAST of CSPI03G39990 vs. TAIR 10
Match: AT4G20050.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 494.6 bits (1272), Expect = 9.0e-140
Identity = 248/424 (58.49%), Postives = 320/424 (75.47%), Query Frame = 0

Query: 53  SLSSAPPS--TAMSGSRVYHVVAYGADPTGESDSTEAIEKAIFDAFEYPTDGHLMKGILD 112
           +L S PP+   A+ G RVY V++YGADPTG+ DST+AI KA+ +AF+ P  G LM+GI D
Sbjct: 58  ALVSPPPTPPQAVPGPRVYQVISYGADPTGKLDSTDAILKAMEEAFDGPNHGVLMQGIND 117

Query: 113 LGGSQLHLDGGTYKISRPLRLPDIPAGNFMIHGGSLRASESFPSNGHLIELRPSSPAVMY 172
           LGG+++ L GG+Y ISRPLR P   AGN +I GG+LRAS  FP + +LIEL+  S  + Y
Sbjct: 118 LGGARIDLQGGSYLISRPLRFPSAGAGNLLISGGTLRASNDFPVDRYLIELKDESSKLQY 177

Query: 173 --EYITLKDLMLNCNFRGGGIAIINSLRTTVDNCYISHF-MSEGIVIEGGHESYIRNSFI 232
             EYITL+DL+++CN+RGG IA+INSLRT++DNCYI+ F  + GI+++ GHE+YIRNSF+
Sbjct: 178 IFEYITLRDLLIDCNYRGGAIAVINSLRTSIDNCYITRFGDTNGILVKSGHETYIRNSFL 237

Query: 233 GQHINVGGDRGEKDFSGIGVKIMGNDNLLRDIVIYSAEIGVMVLGQANVMMGVHCYNKAR 292
           GQHI  GGDRGE+ FSG  + +MGNDN + D VI+SA IGVMV GQAN++ GVHCYNKA 
Sbjct: 238 GQHITAGGDRGERSFSGTAINLMGNDNAVTDTVIFSARIGVMVSGQANLLSGVHCYNKAT 297

Query: 293 ALGGIGIYVKEPGFTQTRIVNCYLDYTGVVVEDPVQVHITGCFFLGNALVVLKSIGGVIS 352
             GG GIY++ PG TQ RIVN YLDYTG+V EDPVQ+ I+G FFLG+A ++LKSI G I 
Sbjct: 298 GFGGTGIYLRLPGLTQNRIVNSYLDYTGIVAEDPVQLQISGTFFLGDAFILLKSIAGYIR 357

Query: 353 GLNIVDNMFSGDYTGVRIVELDESMTPFTRIDQVVVDRNNVRGMVVKSTVGRGSTRANGT 412
           G++IVDNMFSG   GV+IV+LD+  T F  + QVVVDRN+V GMV KSTV RGS   NGT
Sbjct: 358 GVSIVDNMFSGSGHGVQIVQLDQRNTAFDDVGQVVVDRNSVNGMVEKSTVARGSVDGNGT 417

Query: 413 TWTVDFSSLLLFPNLIKNVVYSLEMEQSQGQVFPNHVLTNLTHNRVTVRSNLAIAATLHV 472
           +WTVDF+ +LLFP+LI +V Y+L    S+  VFP H L N++ NRV V +N  +  T++V
Sbjct: 418 SWTVDFNPVLLFPDLINHVQYTL--VASEAGVFPLHALRNVSDNRVVVETNAPVTGTVYV 477

BLAST of CSPI03G39990 vs. TAIR 10
Match: AT4G20050.2 (Pectin lyase-like superfamily protein )

HSP 1 Score: 494.6 bits (1272), Expect = 9.0e-140
Identity = 248/424 (58.49%), Postives = 320/424 (75.47%), Query Frame = 0

Query: 53  SLSSAPPS--TAMSGSRVYHVVAYGADPTGESDSTEAIEKAIFDAFEYPTDGHLMKGILD 112
           +L S PP+   A+ G RVY V++YGADPTG+ DST+AI KA+ +AF+ P  G LM+GI D
Sbjct: 58  ALVSPPPTPPQAVPGPRVYQVISYGADPTGKLDSTDAILKAMEEAFDGPNHGVLMQGIND 117

Query: 113 LGGSQLHLDGGTYKISRPLRLPDIPAGNFMIHGGSLRASESFPSNGHLIELRPSSPAVMY 172
           LGG+++ L GG+Y ISRPLR P   AGN +I GG+LRAS  FP + +LIEL+  S  + Y
Sbjct: 118 LGGARIDLQGGSYLISRPLRFPSAGAGNLLISGGTLRASNDFPVDRYLIELKDESSKLQY 177

Query: 173 --EYITLKDLMLNCNFRGGGIAIINSLRTTVDNCYISHF-MSEGIVIEGGHESYIRNSFI 232
             EYITL+DL+++CN+RGG IA+INSLRT++DNCYI+ F  + GI+++ GHE+YIRNSF+
Sbjct: 178 IFEYITLRDLLIDCNYRGGAIAVINSLRTSIDNCYITRFGDTNGILVKSGHETYIRNSFL 237

Query: 233 GQHINVGGDRGEKDFSGIGVKIMGNDNLLRDIVIYSAEIGVMVLGQANVMMGVHCYNKAR 292
           GQHI  GGDRGE+ FSG  + +MGNDN + D VI+SA IGVMV GQAN++ GVHCYNKA 
Sbjct: 238 GQHITAGGDRGERSFSGTAINLMGNDNAVTDTVIFSARIGVMVSGQANLLSGVHCYNKAT 297

Query: 293 ALGGIGIYVKEPGFTQTRIVNCYLDYTGVVVEDPVQVHITGCFFLGNALVVLKSIGGVIS 352
             GG GIY++ PG TQ RIVN YLDYTG+V EDPVQ+ I+G FFLG+A ++LKSI G I 
Sbjct: 298 GFGGTGIYLRLPGLTQNRIVNSYLDYTGIVAEDPVQLQISGTFFLGDAFILLKSIAGYIR 357

Query: 353 GLNIVDNMFSGDYTGVRIVELDESMTPFTRIDQVVVDRNNVRGMVVKSTVGRGSTRANGT 412
           G++IVDNMFSG   GV+IV+LD+  T F  + QVVVDRN+V GMV KSTV RGS   NGT
Sbjct: 358 GVSIVDNMFSGSGHGVQIVQLDQRNTAFDDVGQVVVDRNSVNGMVEKSTVARGSVDGNGT 417

Query: 413 TWTVDFSSLLLFPNLIKNVVYSLEMEQSQGQVFPNHVLTNLTHNRVTVRSNLAIAATLHV 472
           +WTVDF+ +LLFP+LI +V Y+L    S+  VFP H L N++ NRV V +N  +  T++V
Sbjct: 418 SWTVDFNPVLLFPDLINHVQYTL--VASEAGVFPLHALRNVSDNRVVVETNAPVTGTVYV 477

BLAST of CSPI03G39990 vs. TAIR 10
Match: AT4G20040.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 378.6 bits (971), Expect = 7.2e-105
Identity = 194/426 (45.54%), Postives = 272/426 (63.85%), Query Frame = 0

Query: 67  RVYHVVAYGADPTGESDSTEAIEKAIFDAFEYPTDGHLMKGILDLGGSQLHLDGGTYKIS 126
           +V + + YGADPTG  DS++AI +A+ DAF+  T   ++  + DLGG  + L GG+Y I 
Sbjct: 67  KVIYPIGYGADPTGGQDSSDAILEALTDAFQLQTGLEMLPRVADLGGLVIDLQGGSYMIG 126

Query: 127 RPLRLPDIPAGNFMIHGGSLRASESFPSNGHLIEL--------------------RPSSP 186
           +PLR P    GN ++ GG+ RASE FP + HL+EL                    +  S 
Sbjct: 127 KPLRFPSSGGGNLVVKGGTFRASELFPGDRHLVELVASNAKKPMKMSPEESFSDQKDQSS 186

Query: 187 AVMYEYITLKDLMLNCNFRGGGIAIINSLRTTVDNCYISHFMSEGIVIEGGHESYIRNSF 246
            + YE +T +D++ +  FRGGGI +I+S R  + NCY  HF ++GI ++GGHE+YI NSF
Sbjct: 187 GIFYEDVTFQDVLFDSRFRGGGILVIDSARIRITNCYFLHFTTQGIKVQGGHETYISNSF 246

Query: 247 IGQHINVGGDRGEKDFSGIGVKIMGNDNLLRDIVIYSAEIGVMVLGQANVMMGVHCYNKA 306
           +GQH  VGGDR E+ F+G G+ I  NDN + D+VI+SA IG+ + G AN++ GVHCYNKA
Sbjct: 247 LGQHSTVGGDREERGFTGTGIDISSNDNAITDVVIFSAGIGISLNGGANMVTGVHCYNKA 306

Query: 307 RALGGIGIYVKEPGFTQTRIVNCYLDYTGVVVEDPVQVHITGCFFLGNALVVLKSIGGVI 366
              GGIGI VK      TRI NCYLDYTG+V+EDPV VH+T   FLG+A +VL+S+ G I
Sbjct: 307 TWFGGIGILVKS---HLTRIDNCYLDYTGIVIEDPVHVHVTNALFLGDANIVLRSVHGKI 366

Query: 367 SGLNIVDNMFSGD-YTGVRIVELDESMTPFTRIDQVVVDRNNVRGMVVKSTVGRGSTRAN 426
           SG+NIV+NMFSG       IV+L+     F  I+QVV+D+NN  GM++KST G+    AN
Sbjct: 367 SGVNIVNNMFSGTAKNNFPIVKLEGE---FHDINQVVIDQNNAEGMMLKSTTGKAMVSAN 426

Query: 427 GTTWTVDFSSLLLFPNLIKNVVYSLEMEQSQGQVFPNHVLTNLTHNRVTVRSNLAIAATL 472
           GT W  DFS +L+FPN I +  +S   +  Q    P + +TN+++N V V ++ A+  T+
Sbjct: 427 GTRWIADFSPVLVFPNRINHYQHSFFAQSGQ---IPANAVTNVSNNMVVVETDRAVTGTV 483

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O494321.3e-13858.49Polygalacturonase QRT3 OS=Arabidopsis thaliana OX=3702 GN=QRT3 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LD068.2e-274100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G837050 PE=4 SV=1[more]
A0A5D3BRF53.5e-25693.83Polygalacturonase QRT3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf... [more]
A0A1S3B7S63.5e-25693.83polygalacturonase QRT3-like OS=Cucumis melo OX=3656 GN=LOC103487092 PE=4 SV=1[more]
A0A6J1J1V13.3e-23885.28polygalacturonase QRT3-like OS=Cucurbita maxima OX=3661 GN=LOC111481950 PE=4 SV=... [more]
A0A6J1F3153.3e-23885.71polygalacturonase QRT3-like OS=Cucurbita moschata OX=3662 GN=LOC111441905 PE=4 S... [more]
Match NameE-valueIdentityDescription
XP_004141189.21.7e-273100.00polygalacturonase QRT3 [Cucumis sativus] >KGN59673.1 hypothetical protein Csa_00... [more]
XP_008443524.27.2e-25693.83PREDICTED: polygalacturonase QRT3-like [Cucumis melo] >KAA0053667.1 polygalactur... [more]
XP_038905582.11.0e-24690.48polygalacturonase QRT3-like [Benincasa hispida][more]
XP_022983336.16.7e-23885.28polygalacturonase QRT3-like [Cucurbita maxima][more]
XP_022934866.16.7e-23885.71polygalacturonase QRT3-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT4G20050.19.0e-14058.49Pectin lyase-like superfamily protein [more]
AT4G20050.29.0e-14058.49Pectin lyase-like superfamily protein [more]
AT4G20040.17.2e-10545.54Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 69..437
e-value: 4.7E-24
score: 87.1
NoneNo IPR availablePANTHERPTHR33928:SF4POLYGALACTURONASE QRT3-LIKEcoord: 3..475
IPR039279Polygalacturonase QRT3-likePANTHERPTHR33928POLYGALACTURONASE QRT3coord: 3..475
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 64..398

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI03G39990.1CSPI03G39990.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016829 lyase activity
molecular_function GO:0004650 polygalacturonase activity