CSPI03G38820 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI03G38820
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionDihydroorotase
LocationChr3: 33434310 .. 33437608 (-)
RNA-Seq ExpressionCSPI03G38820
SyntenyCSPI03G38820
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTAATTATAACAACATCCATAAAAAAACTATTGAAAATTGTATGGATTTCATTTCCAATTAACACCATATTTCAAACCTTGTTAGTAAGGGCTTCAATTTTGTCTTATTTGTTTAATTATCCATCCCATCTTTCCTCAAGCTCCAAGCACCTCACGGCGAATCCACCATCTCCGGCTATCCTCAGGCGACCCAACTCAATCACAAAGAGCCGGACCGTCCTGGCTCTTCCTACTCCGGCGGATTCAGTTCCTCGCGGCAGCAGATCTACAAGTGCAAGTGACCCACGAACGGCTCCTGCGACGTTTCCCACTTCTTCTTCTTCTTCTCCGGGCAACAAGCTGCACCGGCGCTAGGTAAGCTTCGAAAACAAATCAACAACGGCGGCATTTGGTTGTGCATCTGTTATCTCTGATTTTATATTTTCATTTGAACAGAAGAAAAATCGTTTTAAAGAAGCCTCAAGGTTGTCTCTGGATTTTACTATGACAAAGACTTTGGTCCTTCCGTTCCAAGTACATATGCTTTAGTTATCAATAAAATCCAATCTATTTTATATTCAATTTGTATTTGGTTCATTTTGTTGTCTGTCATCAACTCTTCTTCGAGCAACGTTCATGTGGCGTATTATAGTTCAAGCATTGTAGGATGTACTTTGAGCGTCATCTTCGGTGTGATATTTTCGTAGTTTCCTTAACCAATGACAATTTACTAAACTTAAATTCAGATCTCTGCTTTCATTTCACTGATTGCTACATTGTTGATGGTAGCCACTGAAATCACCTGCACAAAAGTTTGAAGGGGTTAAGTTCATAAGGCGCCAAGGGCCAAAAATGCAGCTCACACTCACCCAGCCTGATGATTGGCATCTTCATCTTCGCGATGGTGATCTCTTAAAAGCGGTCCTCCCTCACAGGTAAGCTTATCTTTTATTCTTTGTACATGGTGGTAGTGGTTTTGAAATGATTAAAATCACTTTTGTCAAGATTAAGTTACTCTAATACATGCTTTCAATCATTCATAGTCAATTTTAGTTGTATAAAAATAATGTTTAAAAGTGTAAAATCCAGCATTGGGTTGCTTTTCATTGATTGAGATATGTTTATGGGTGATTTTGAAGGTGTCAACAGTTTTAGAATCACTCCTAAACATATCATAATTGACATGTCAAGCAACCACATGATGCATTCTATTTTAACATGTATATCATGTTTTCAATTGTTCATTCTTGTCCTAAACTATCAATATGCAGTGCAAGTACCTTTGGAAGGGCTATTGTGATGCCAAATCTGAAGCCACCTGTCACTACCACAGCTGCTGCTGTGGCGTATCGGGATTCTATCTTGAAAGCGTTACCGTCCAATAGCAACTTTGTTCCTCTTATGACCCTTTATTTGACGGATACAATGAGCCCAAACGAGATCAAGCTTGCAAGTAAGTCTTTGCTTTCAACAAGAAATTTTGGAAGAGATCTGTTATTTGATGATTTTTGAGGTTGTATTCTATTCTTTTTTCTAACATGATGATGGATTGACAGGAAAAAGTGGGGCCGTTTATGCTGTAAAATTGTATCCTGCCGGTGCTACGACCAATTCCCAAGATGGTGTTACAGATCTTTTTGGGAAGTGTCTACCTGTTCTTGAGGAGATGGTTGAACAGGATATGCCTCTGTTGGTAGTTTGCTGATAGGAACACTTTGTTTATTCGTCCAATATATAGAGAAGCAGTGCATTCTTTCTTCTTGTTAATTTAAGAAGAGATATCTCTCTGTAGGTTCATGGTGAGGTAACAAATTCTGATGTTGACATGTTTGATCGTGAGAAGGTCTTCATTGAAACTGTGTTGAAACCCCTGATTGAGAGGCTTCCAAAGCTGAAGGTGGTGATGGAGCATATTACTACTGCAGATGCTGCTAAGTTTGTTTTGTCTTGCGAACATGGTATTTATACTTCATTATTTTCATTGAAGTGTTTTTGTTAGTTATCCCTCTTTCATTTTGTCGACCATTGGTATCACTTTTTTCTTGTAAATTATGCTTACCAATATATGACTTTTAAAATCCTTTACAGCGCTTCTAGCAGCAACTGTCACTCCACAACATCTTCTTCTCAACAGGAATTCTCTATTTCAAGGAGGACTGCAACCACATAATTACTGTCTTCCGGTACTCAAAAGAGAGACTCACAGTATGTTCCAACTATATTTTGTGCTTATTCTTTATATTTTCCTCACACATGTTCTTCTTGTATGACTGTATCAGTAGCCTTCATGAAAAACAATTTCTCCTTACAAGTATAAGATATTCTTATCCAAGTTAGAAGGTTTTTTGGAGGTTAGGATGGATTAACCTCAACATAAGGTGGCTTTTGATATTCGTCATGTATCTTAGAATCATTGAAGTTGGCCTAGTGGTCAATATTAATTATAAAAGGCTTTGGGTGAATGAGTTCAAGCCATGGTTGTCACCTTCTTAAGATTTAATACCTCATGAGTTTTTTTGTTTGGACTTTTCTTCATGAAACTTGAGAATCCCCCAAAAGAAAGATAATTATTTCTTCATTTGTGCTCACGCCTTTTCTCTCATCTAGGGCAGGCAATTGTGTCAGCTGTAACAAGCGGAGGCAAAAAGTTCTTCCTCGGAACTGACAGCGCTCCACATGAGAGAAAAAGGAAAGAGAGTTCATGTGGTTGTGCCGGTATATACAGTGCCCCTATTGCCTTGTCATTATATGCAAAGGTGTTTGAAGAGGTATACCATCTTGTTTCTTATTATGCTAATAATTTGAATCACTTCATCTTTATCAAATAATCATCATTTTCTCCTTTTTGGTTTCTTTTTCTCTGCCAAGGCGGGTGCTCTTGATAAACTAGAAGCATTTACAAGTTTCAATGGACCCGACTTCTATGGACTCCCCAGAAACACCTCAAAAATTACACTGCAAAAGGCTCCATGGCAGGTCCCAAAGTCTTTTTCATTTTCTTTTGGGGATATTGTTCCAATGTTTGCAGGTGAGACACTTGAGTGGCAGCCATGCTTCAACTGAGACTAACAAGTTCCAATGTTGCTTGCTTACACAATAACACCACATTCTAAGCATGTCCTCAATTCTCTTGTTTAACTACACTGCTGTTGATTTACCTGAATTAGAGCTTTTTACTGTTGGGACATCTATGATTGTTCCCTTCCCTCCCATTTTTGAAATGAGATCATGATGAACTTTTGATTTGTATGAGATTTTGAAGAAGTTGATCTTGGATATTGTTGGTTTGGTAATAAAAATTCCCAAAATTTGTTTTTGG

mRNA sequence

ATTAATTATAACAACATCCATAAAAAAACTATTGAAAATTGTATGGATTTCATTTCCAATTAACACCATATTTCAAACCTTGTTAGTAAGGGCTTCAATTTTGTCTTATTTGTTTAATTATCCATCCCATCTTTCCTCAAGCTCCAAGCACCTCACGGCGAATCCACCATCTCCGGCTATCCTCAGGCGACCCAACTCAATCACAAAGAGCCGGACCGTCCTGGCTCTTCCTACTCCGGCGGATTCAGTTCCTCGCGGCAGCAGATCTACAAGTGCAAGTGACCCACGAACGGCTCCTGCGACGTTTCCCACTTCTTCTTCTTCTTCTCCGGGCAACAAGCTGCACCGGCGCTAGAAGAAAAATCGTTTTAAAGAAGCCTCAAGGTTGTCTCTGGATTTTACTATGACAAAGACTTTGGTCCTTCCGTTCCAACCACTGAAATCACCTGCACAAAAGTTTGAAGGGGTTAAGTTCATAAGGCGCCAAGGGCCAAAAATGCAGCTCACACTCACCCAGCCTGATGATTGGCATCTTCATCTTCGCGATGGTGATCTCTTAAAAGCGGTCCTCCCTCACAGTGCAAGTACCTTTGGAAGGGCTATTGTGATGCCAAATCTGAAGCCACCTGTCACTACCACAGCTGCTGCTGTGGCGTATCGGGATTCTATCTTGAAAGCGTTACCGTCCAATAGCAACTTTGTTCCTCTTATGACCCTTTATTTGACGGATACAATGAGCCCAAACGAGATCAAGCTTGCAAGAAAAAGTGGGGCCGTTTATGCTGTAAAATTGTATCCTGCCGGTGCTACGACCAATTCCCAAGATGGTGTTACAGATCTTTTTGGGAAGTGTCTACCTGTTCTTGAGGAGATGGTTGAACAGGATATGCCTCTGTTGGTTCATGGTGAGGTAACAAATTCTGATGTTGACATGTTTGATCGTGAGAAGGTCTTCATTGAAACTGTGTTGAAACCCCTGATTGAGAGGCTTCCAAAGCTGAAGGTGGTGATGGAGCATATTACTACTGCAGATGCTGCTAAGTTTGTTTTGTCTTGCGAACATGCGCTTCTAGCAGCAACTGTCACTCCACAACATCTTCTTCTCAACAGGAATTCTCTATTTCAAGGAGGACTGCAACCACATAATTACTGTCTTCCGGTACTCAAAAGAGAGACTCACAGGCAGGCAATTGTGTCAGCTGTAACAAGCGGAGGCAAAAAGTTCTTCCTCGGAACTGACAGCGCTCCACATGAGAGAAAAAGGAAAGAGAGTTCATGTGGTTGTGCCGGTATATACAGTGCCCCTATTGCCTTGTCATTATATGCAAAGGTGTTTGAAGAGGCGGGTGCTCTTGATAAACTAGAAGCATTTACAAGTTTCAATGGACCCGACTTCTATGGACTCCCCAGAAACACCTCAAAAATTACACTGCAAAAGGCTCCATGGCAGGTCCCAAAGTCTTTTTCATTTTCTTTTGGGGATATTGTTCCAATGTTTGCAGGTGAGACACTTGAGTGGCAGCCATGCTTCAACTGAGACTAACAAGTTCCAATGTTGCTTGCTTACACAATAACACCACATTCTAAGCATGTCCTCAATTCTCTTGTTTAACTACACTGCTGTTGATTTACCTGAATTAGAGCTTTTTACTGTTGGGACATCTATGATTGTTCCCTTCCCTCCCATTTTTGAAATGAGATCATGATGAACTTTTGATTTGTATGAGATTTTGAAGAAGTTGATCTTGGATATTGTTGGTTTGGTAATAAAAATTCCCAAAATTTGTTTTTGG

Coding sequence (CDS)

ATGACAAAGACTTTGGTCCTTCCGTTCCAACCACTGAAATCACCTGCACAAAAGTTTGAAGGGGTTAAGTTCATAAGGCGCCAAGGGCCAAAAATGCAGCTCACACTCACCCAGCCTGATGATTGGCATCTTCATCTTCGCGATGGTGATCTCTTAAAAGCGGTCCTCCCTCACAGTGCAAGTACCTTTGGAAGGGCTATTGTGATGCCAAATCTGAAGCCACCTGTCACTACCACAGCTGCTGCTGTGGCGTATCGGGATTCTATCTTGAAAGCGTTACCGTCCAATAGCAACTTTGTTCCTCTTATGACCCTTTATTTGACGGATACAATGAGCCCAAACGAGATCAAGCTTGCAAGAAAAAGTGGGGCCGTTTATGCTGTAAAATTGTATCCTGCCGGTGCTACGACCAATTCCCAAGATGGTGTTACAGATCTTTTTGGGAAGTGTCTACCTGTTCTTGAGGAGATGGTTGAACAGGATATGCCTCTGTTGGTTCATGGTGAGGTAACAAATTCTGATGTTGACATGTTTGATCGTGAGAAGGTCTTCATTGAAACTGTGTTGAAACCCCTGATTGAGAGGCTTCCAAAGCTGAAGGTGGTGATGGAGCATATTACTACTGCAGATGCTGCTAAGTTTGTTTTGTCTTGCGAACATGCGCTTCTAGCAGCAACTGTCACTCCACAACATCTTCTTCTCAACAGGAATTCTCTATTTCAAGGAGGACTGCAACCACATAATTACTGTCTTCCGGTACTCAAAAGAGAGACTCACAGGCAGGCAATTGTGTCAGCTGTAACAAGCGGAGGCAAAAAGTTCTTCCTCGGAACTGACAGCGCTCCACATGAGAGAAAAAGGAAAGAGAGTTCATGTGGTTGTGCCGGTATATACAGTGCCCCTATTGCCTTGTCATTATATGCAAAGGTGTTTGAAGAGGCGGGTGCTCTTGATAAACTAGAAGCATTTACAAGTTTCAATGGACCCGACTTCTATGGACTCCCCAGAAACACCTCAAAAATTACACTGCAAAAGGCTCCATGGCAGGTCCCAAAGTCTTTTTCATTTTCTTTTGGGGATATTGTTCCAATGTTTGCAGGTGAGACACTTGAGTGGCAGCCATGCTTCAACTGA

Protein sequence

MTKTLVLPFQPLKSPAQKFEGVKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKAVLPHSASTFGRAIVMPNLKPPVTTTAAAVAYRDSILKALPSNSNFVPLMTLYLTDTMSPNEIKLARKSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEQDMPLLVHGEVTNSDVDMFDREKVFIETVLKPLIERLPKLKVVMEHITTADAAKFVLSCEHALLAATVTPQHLLLNRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSGGKKFFLGTDSAPHERKRKESSCGCAGIYSAPIALSLYAKVFEEAGALDKLEAFTSFNGPDFYGLPRNTSKITLQKAPWQVPKSFSFSFGDIVPMFAGETLEWQPCFN*
Homology
BLAST of CSPI03G38820 vs. ExPASy Swiss-Prot
Match: O04904 (Dihydroorotase, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=PYR4 PE=1 SV=1)

HSP 1 Score: 599.4 bits (1544), Expect = 2.9e-170
Identity = 292/375 (77.87%), Postives = 329/375 (87.73%), Query Frame = 0

Query: 1   MTKTLVLPFQPLKSPAQKFE-GVKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKAVLPHS 60
           M KTLV P+    S   KF+   + ++ +  +M+LT+TQPDDWHLHLRDGDLL AV+PHS
Sbjct: 1   MIKTLVSPYSGFGSQKLKFDRSSEKVKTRAVRMELTITQPDDWHLHLRDGDLLHAVVPHS 60

Query: 61  ASTFGRAIVMPNLKPPVTTTAAAVAYRDSILKALPSNSNFVPLMTLYLTDTMSPNEIKLA 120
           AS F RAIVMPNLKPPVT+TAAA+ YR  I+KALPS S+F PLMTLYLTD   P EI+LA
Sbjct: 61  ASNFKRAIVMPNLKPPVTSTAAAIIYRKFIMKALPSESSFDPLMTLYLTDKTLPEEIRLA 120

Query: 121 RKSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEQDMPLLVHGEVTNSDVDMFD 180
           R+SG VYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMV+Q+MPLLVHGEVT+  +D+FD
Sbjct: 121 RESGVVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVKQNMPLLVHGEVTDPSIDVFD 180

Query: 181 REKVFIETVLKPLIERLPKLKVVMEHITTADAAKFVLSCEHALLAATVTPQHLLLNRNSL 240
           REK+FIETVL+PLI+RLP+LKVVMEHITT DA  FV SC+   + ATVTPQHLLLNRN+L
Sbjct: 181 REKIFIETVLQPLIQRLPQLKVVMEHITTMDAVNFVESCKEGSVGATVTPQHLLLNRNAL 240

Query: 241 FQGGLQPHNYCLPVLKRETHRQAIVSAVTSGGKKFFLGTDSAPHERKRKESSCGCAGIYS 300
           FQGGLQPHNYCLPVLKRE HR+AIV AVTSG KKFFLGTDSAPHER RKESSCGCAGIYS
Sbjct: 241 FQGGLQPHNYCLPVLKREIHREAIVKAVTSGSKKFFLGTDSAPHERSRKESSCGCAGIYS 300

Query: 301 APIALSLYAKVFEEAGALDKLEAFTSFNGPDFYGLPRNTSKITLQKAPWQVPKSFSFSFG 360
           APIALSLYAKVF+EAGALDKLEAFTSFNGPDFYGLPRN+SKITL+K+PW+VP  F+F FG
Sbjct: 301 APIALSLYAKVFDEAGALDKLEAFTSFNGPDFYGLPRNSSKITLKKSPWKVPDVFNFPFG 360

Query: 361 DIVPMFAGETLEWQP 375
           +IVPMFAGETL+WQP
Sbjct: 361 EIVPMFAGETLQWQP 375

BLAST of CSPI03G38820 vs. ExPASy Swiss-Prot
Match: Q0JJD1 (Dihydroorotase, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=PYRC PE=2 SV=1)

HSP 1 Score: 573.9 bits (1478), Expect = 1.3e-162
Identity = 272/345 (78.84%), Postives = 308/345 (89.28%), Query Frame = 0

Query: 30  PKMQLTLTQPDDWHLHLRDGDLLKAVLPHSASTFGRAIVMPNLKPPVTTTAAAVAYRDSI 89
           P  +LT+T+PDDWHLHLR+GD+L AVLPHSA  FGRAIVMPNLKPPVTTTA A+ YR+ I
Sbjct: 56  PLQELTITRPDDWHLHLREGDVLAAVLPHSAMHFGRAIVMPNLKPPVTTTARALEYREEI 115

Query: 90  LKALPSNSNFVPLMTLYLTDTMSPNEIKLARKSGAVYAVKLYPAGATTNSQDGVTDLFGK 149
           L+ALP  SNFVPLMTLYLTD  SP EIKLA+KSG V+AVKLYP+GATTNSQDGVTD+FGK
Sbjct: 116 LRALPPGSNFVPLMTLYLTDNTSPEEIKLAKKSGVVFAVKLYPSGATTNSQDGVTDIFGK 175

Query: 150 CLPVLEEMVEQDMPLLVHGEVTNSDVDMFDREKVFIETVLKPLIERLPKLKVVMEHITTA 209
           CLPVLEEM  Q+MPLLVHGEVT+  VD FDREKVFIE +L PL++RLP+LK+VMEHITT 
Sbjct: 176 CLPVLEEMARQEMPLLVHGEVTDQHVDTFDREKVFIEKILAPLVQRLPQLKIVMEHITTM 235

Query: 210 DAAKFVLSCEHALLAATVTPQHLLLNRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTS 269
           DA  FV SC+   +AATVTPQHLLLNRN+LFQGGLQPHNYCLPVLKRETHRQAIVSAVTS
Sbjct: 236 DAVNFVESCKEGHVAATVTPQHLLLNRNALFQGGLQPHNYCLPVLKRETHRQAIVSAVTS 295

Query: 270 GGKKFFLGTDSAPHERKRKESSCGCAGIYSAPIALSLYAKVFEEAGALDKLEAFTSFNGP 329
           G K++FLGTDSAPH+++RKE SCGCAGIYSAP+ALSLYAKVFE+AGALDKLEAFTSFNGP
Sbjct: 296 GSKQYFLGTDSAPHDKRRKECSCGCAGIYSAPVALSLYAKVFEQAGALDKLEAFTSFNGP 355

Query: 330 DFYGLPRNTSKITLQKAPWQVPKSFSFSFGDIVPMFAGETLEWQP 375
           DFYGLPRNTSKI L+K+ W+VP ++S+S G+IVPMF G TLEW P
Sbjct: 356 DFYGLPRNTSKIVLRKSAWKVPDTYSYSSGEIVPMFTGNTLEWLP 400

BLAST of CSPI03G38820 vs. ExPASy Swiss-Prot
Match: B1XI96 (Dihydroorotase OS=Synechococcus sp. (strain ATCC 27264 / PCC 7002 / PR-6) OX=32049 GN=pyrC PE=3 SV=1)

HSP 1 Score: 453.8 bits (1166), Expect = 2.0e-126
Identity = 224/341 (65.69%), Postives = 262/341 (76.83%), Query Frame = 0

Query: 33  QLTLTQPDDWHLHLRDGDLLKAVLPHSASTFGRAIVMPNLKPPVTTTAAAVAYRDSILKA 92
           ++TLT+PDDWHLHLRDG+ LKAVLP +   F RAIVMPNLKPPV T A A AY D IL A
Sbjct: 3   KITLTRPDDWHLHLRDGEALKAVLPDTVRQFARAIVMPNLKPPVRTVAEAAAYHDRILAA 62

Query: 93  LPSNSNFVPLMTLYLTDTMSPNEIKLARKSGAVYAVKLYPAGATTNSQDGVTDLFGKCLP 152
           +P    F PLMTLYLTD  SP EI+ A++SG V AVK YPAGATTNS  GVTD+  KC  
Sbjct: 63  IPPGRTFEPLMTLYLTDNTSPAEIQAAKESGFVKAVKYYPAGATTNSDLGVTDI-RKCDA 122

Query: 153 VLEEMVEQDMPLLVHGEVTNSDVDMFDREKVFIETVLKPLIERLPKLKVVMEHITTADAA 212
           V E M   D+PLL+HGEVT+  VD+FDREKVFIET L+PL ER P+L++V+EHITT DA 
Sbjct: 123 VFEAMQTVDLPLLLHGEVTDHRVDVFDREKVFIETYLRPLKERFPQLRIVLEHITTKDAV 182

Query: 213 KFVLSCEHALLAATVTPQHLLLNRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSGGK 272
           +FVL+ +   +AAT+TPQHLL NRNS+FQGG++PH YCLP+LKRETHR+A++ A TSG  
Sbjct: 183 EFVLASDEK-VAATITPQHLLFNRNSIFQGGIRPHFYCLPILKRETHREALLEAATSGNP 242

Query: 273 KFFLGTDSAPHERKRKESSCGCAGIYSAPIALSLYAKVFEEAGALDKLEAFTSFNGPDFY 332
           KFFLGTDSAPH R RKES CGCAG YSA  AL LYA VFE A ALDKLE F SF GPDFY
Sbjct: 243 KFFLGTDSAPHGRDRKESDCGCAGCYSALHALELYATVFEAADALDKLEGFASFYGPDFY 302

Query: 333 GLPRNTSKITLQKAPWQVPKSFSFSFGDIVPMFAGETLEWQ 374
            LPRNT KITL K PWQ+P +  F    +VP++AG+ L W+
Sbjct: 303 QLPRNTEKITLTKTPWQIPDALPFPESSLVPLWAGQELSWK 341

BLAST of CSPI03G38820 vs. ExPASy Swiss-Prot
Match: C1D672 (Dihydroorotase OS=Laribacter hongkongensis (strain HLHK9) OX=557598 GN=pyrC PE=3 SV=1)

HSP 1 Score: 443.0 bits (1138), Expect = 3.5e-123
Identity = 223/341 (65.40%), Postives = 259/341 (75.95%), Query Frame = 0

Query: 34  LTLTQPDDWHLHLRDGDLLKAVLPHSASTFGRAIVMPNLKPPVTTTAAAVAYRDSILKAL 93
           LTLT+PDDWHLHLRDG+ L AV  +SA  F RAIVMPNLKPPVTT   A AYR+ IL AL
Sbjct: 4   LTLTRPDDWHLHLRDGEALAAVAAYSAREFARAIVMPNLKPPVTTVVQAAAYRERILAAL 63

Query: 94  -PSNSNFVPLMTLYLTDTMSPNEIKLARKSGAVYAVKLYPAGATTNSQDGVTDLFGKCLP 153
            P    F PLMTLYLTD   P EI    +SG V+A+KLYPAGATTNS  GVT L G CLP
Sbjct: 64  PPGGPAFEPLMTLYLTDNTPPAEIDRVAESGFVHALKLYPAGATTNSDAGVTSLSG-CLP 123

Query: 154 VLEEMVEQDMPLLVHGEVTNSDVDMFDREKVFIETVLKPLIERLPKLKVVMEHITTADAA 213
            L  M E  +PLLVHGEVT+  +D+FDRE VFI+TVL+PL+  LP L+VVMEHITT  AA
Sbjct: 124 TLTRMAELGVPLLVHGEVTDPQIDVFDREAVFIDTVLQPLLAELPTLRVVMEHITTRQAA 183

Query: 214 KFVLSCEHALLAATVTPQHLLLNRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSGGK 273
           +FVL+     +AATVTPQH+L+NRN+LF GGL+PH+YCLPVLKRE HR AI  AVTSG  
Sbjct: 184 EFVLAAPDN-IAATVTPQHMLMNRNALFTGGLRPHHYCLPVLKREEHRAAIARAVTSGST 243

Query: 274 KFFLGTDSAPHERKRKESSCGCAGIYSAPIALSLYAKVFEEAGALDKLEAFTSFNGPDFY 333
           +FFLGTDSAPH R  KE++CGCAGI+SA  AL LYA+ FE+AGALD+LEAF SFNGPDFY
Sbjct: 244 RFFLGTDSAPHARHAKEAACGCAGIFSAHAALPLYAEAFEDAGALDRLEAFASFNGPDFY 303

Query: 334 GLPRNTSKITLQKAPWQVPKSFSFSFGDIVPMFAGETLEWQ 374
            LPRNT  +TL++  W VP S  +    +VP+ AGETL WQ
Sbjct: 304 RLPRNTGTVTLKREEWTVPASLPYGNDTLVPLRAGETLRWQ 342

BLAST of CSPI03G38820 vs. ExPASy Swiss-Prot
Match: B0CF78 (Dihydroorotase OS=Acaryochloris marina (strain MBIC 11017) OX=329726 GN=pyrC PE=3 SV=1)

HSP 1 Score: 438.3 bits (1126), Expect = 8.6e-122
Identity = 217/341 (63.64%), Postives = 256/341 (75.07%), Query Frame = 0

Query: 33  QLTLTQPDDWHLHLRDGDLLKAVLPHSASTFGRAIVMPNLKPPVTTTAAAVAYRDSILKA 92
           +LTLT+PDDWHLHLRDGD LKAVLPHSA  F RAIVMPNLKPPV T A A  YR+ IL A
Sbjct: 3   KLTLTRPDDWHLHLRDGDALKAVLPHSARQFARAIVMPNLKPPVRTVADAADYRNRILAA 62

Query: 93  LPSNSNFVPLMTLYLTDTMSPNEIKLARKSGAVYAVKLYPAGATTNSQDGVTDLFGKCLP 152
           LP    F PLMTLYLTD  SP EI  A+ S  V AVK YPAGATTNS  GVTD+  KC  
Sbjct: 63  LPVGQTFEPLMTLYLTDNTSPEEISAAKASKFVKAVKYYPAGATTNSDFGVTDI-RKCDR 122

Query: 153 VLEEMVEQDMPLLVHGEVTNSDVDMFDREKVFIETVLKPLIERLPKLKVVMEHITTADAA 212
           +   M ++D+PLL+HGEVT++++D+FDREKVFIET L PL+ R PKL+VV+EHITT++A 
Sbjct: 123 IFAAMEQEDIPLLLHGEVTDTNIDVFDREKVFIETHLIPLVSRFPKLRVVLEHITTSEAV 182

Query: 213 KFVLSCEHALLAATVTPQHLLLNRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSGGK 272
            FVL+     + AT+TPQHLL +RN++F+GG++PH YCLP+LKRE HRQA++ A TSG  
Sbjct: 183 TFVLNTNEN-IGATITPQHLLFSRNAIFKGGIRPHYYCLPILKREKHRQALLQAATSGNP 242

Query: 273 KFFLGTDSAPHERKRKESSCGCAGIYSAPIALSLYAKVFEEAGALDKLEAFTSFNGPDFY 332
           KFFLGTDSAPH R  KE SCGCAG YSA  A+ LYA+ FE A ALDKLEAF SF GPDFY
Sbjct: 243 KFFLGTDSAPHARNSKEQSCGCAGCYSALHAMELYAEAFESANALDKLEAFASFYGPDFY 302

Query: 333 GLPRNTSKITLQKAPWQVPKSFSFSFGDIVPMFAGETLEWQ 374
            LPRNT  ITL K  WQ+P    F    +VP+ AGE + W+
Sbjct: 303 QLPRNTKTITLAKQTWQIPDEVPFPGTGLVPLRAGEEITWK 341

BLAST of CSPI03G38820 vs. ExPASy TrEMBL
Match: A0A0A0LG11 (Dihydroorotase OS=Cucumis sativus OX=3659 GN=Csa_3G824970 PE=3 SV=1)

HSP 1 Score: 757.3 bits (1954), Expect = 3.1e-215
Identity = 374/377 (99.20%), Postives = 375/377 (99.47%), Query Frame = 0

Query: 1   MTKTLVLPFQPLKSPAQKFEGVKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKAVLPHSA 60
           MTKTLVLPFQPLKSP QKFEGVKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKAVLPHSA
Sbjct: 1   MTKTLVLPFQPLKSPGQKFEGVKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKAVLPHSA 60

Query: 61  STFGRAIVMPNLKPPVTTTAAAVAYRDSILKALPSNSNFVPLMTLYLTDTMSPNEIKLAR 120
           STFGRAIVMPNLKPPVTTTAAAVAYRDSILKALPSNSNFVPLMTLYLTDTMSPNEIKLAR
Sbjct: 61  STFGRAIVMPNLKPPVTTTAAAVAYRDSILKALPSNSNFVPLMTLYLTDTMSPNEIKLAR 120

Query: 121 KSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEQDMPLLVHGEVTNSDVDMFDR 180
           KSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEQDMPLLVHGEVTNSDVDMFDR
Sbjct: 121 KSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEQDMPLLVHGEVTNSDVDMFDR 180

Query: 181 EKVFIETVLKPLIERLPKLKVVMEHITTADAAKFVLSCEHALLAATVTPQHLLLNRNSLF 240
           EKVFIETVLKPLIERLPKLKVVMEHITTADAAKFVLSCEHALLAATVTPQHLLLNRNSLF
Sbjct: 181 EKVFIETVLKPLIERLPKLKVVMEHITTADAAKFVLSCEHALLAATVTPQHLLLNRNSLF 240

Query: 241 QGGLQPHNYCLPVLKRETHRQAIVSAVTSGGKKFFLGTDSAPHERKRKESSCGCAGIYSA 300
           QGGLQPHNYCLPVLKRETHRQAIVSAVTSG KKFFLGTDSAPHERKRKESSCGCAGIYSA
Sbjct: 241 QGGLQPHNYCLPVLKRETHRQAIVSAVTSGSKKFFLGTDSAPHERKRKESSCGCAGIYSA 300

Query: 301 PIALSLYAKVFEEAGALDKLEAFTSFNGPDFYGLPRNTSKITLQKAPWQVPKSFSFSFGD 360
           PIALSLYAKVFEEAGALDKLE+FTSFNGPDFYGLPRNTSKITLQKAPWQVPKSFSFSFGD
Sbjct: 301 PIALSLYAKVFEEAGALDKLESFTSFNGPDFYGLPRNTSKITLQKAPWQVPKSFSFSFGD 360

Query: 361 IVPMFAGETLEWQPCFN 378
           IVPMFAGETLEWQPCFN
Sbjct: 361 IVPMFAGETLEWQPCFN 377

BLAST of CSPI03G38820 vs. ExPASy TrEMBL
Match: A0A5D3DPM6 (Dihydroorotase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G007860 PE=3 SV=1)

HSP 1 Score: 751.1 bits (1938), Expect = 2.2e-213
Identity = 369/377 (97.88%), Postives = 374/377 (99.20%), Query Frame = 0

Query: 1   MTKTLVLPFQPLKSPAQKFEGVKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKAVLPHSA 60
           MTKTLVLPFQPLKSPAQKFEGVKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKAVLPHSA
Sbjct: 88  MTKTLVLPFQPLKSPAQKFEGVKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKAVLPHSA 147

Query: 61  STFGRAIVMPNLKPPVTTTAAAVAYRDSILKALPSNSNFVPLMTLYLTDTMSPNEIKLAR 120
           STFGRAIVMPNLKPP+TTTAAAVAYRDSILKALPSNSNF+PLMTLYLTDTMSPNEIKLAR
Sbjct: 148 STFGRAIVMPNLKPPITTTAAAVAYRDSILKALPSNSNFIPLMTLYLTDTMSPNEIKLAR 207

Query: 121 KSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEQDMPLLVHGEVTNSDVDMFDR 180
           KSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEQDMPLLVHGEVTN DVDMFDR
Sbjct: 208 KSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEQDMPLLVHGEVTNPDVDMFDR 267

Query: 181 EKVFIETVLKPLIERLPKLKVVMEHITTADAAKFVLSCEHALLAATVTPQHLLLNRNSLF 240
           EKVFIETVLKPLIERLPKLKVVMEHITTA+AAKFVLSCEHALLAATVTPQHLLLNRNSLF
Sbjct: 268 EKVFIETVLKPLIERLPKLKVVMEHITTANAAKFVLSCEHALLAATVTPQHLLLNRNSLF 327

Query: 241 QGGLQPHNYCLPVLKRETHRQAIVSAVTSGGKKFFLGTDSAPHERKRKESSCGCAGIYSA 300
           QGGLQPHNYCLPVLKRETHRQAIVSAVTSG KKFFLGTDSAPHER+RKESSCGCAGIYSA
Sbjct: 328 QGGLQPHNYCLPVLKRETHRQAIVSAVTSGSKKFFLGTDSAPHERRRKESSCGCAGIYSA 387

Query: 301 PIALSLYAKVFEEAGALDKLEAFTSFNGPDFYGLPRNTSKITLQKAPWQVPKSFSFSFGD 360
           PIALSLYAKVFEEAGALDKLEAFTSFNGPDFYGLPRNTSKITLQKAPWQVPKSF+F FGD
Sbjct: 388 PIALSLYAKVFEEAGALDKLEAFTSFNGPDFYGLPRNTSKITLQKAPWQVPKSFTFPFGD 447

Query: 361 IVPMFAGETLEWQPCFN 378
           IVPMFAGETLEWQPCFN
Sbjct: 448 IVPMFAGETLEWQPCFN 464

BLAST of CSPI03G38820 vs. ExPASy TrEMBL
Match: A0A1S3B7W2 (Dihydroorotase OS=Cucumis melo OX=3656 GN=LOC103486989 PE=3 SV=1)

HSP 1 Score: 751.1 bits (1938), Expect = 2.2e-213
Identity = 369/377 (97.88%), Postives = 374/377 (99.20%), Query Frame = 0

Query: 1   MTKTLVLPFQPLKSPAQKFEGVKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKAVLPHSA 60
           MTKTLVLPFQPLKSPAQKFEGVKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKAVLPHSA
Sbjct: 1   MTKTLVLPFQPLKSPAQKFEGVKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKAVLPHSA 60

Query: 61  STFGRAIVMPNLKPPVTTTAAAVAYRDSILKALPSNSNFVPLMTLYLTDTMSPNEIKLAR 120
           STFGRAIVMPNLKPP+TTTAAAVAYRDSILKALPSNSNF+PLMTLYLTDTMSPNEIKLAR
Sbjct: 61  STFGRAIVMPNLKPPITTTAAAVAYRDSILKALPSNSNFIPLMTLYLTDTMSPNEIKLAR 120

Query: 121 KSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEQDMPLLVHGEVTNSDVDMFDR 180
           KSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEQDMPLLVHGEVTN DVDMFDR
Sbjct: 121 KSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEQDMPLLVHGEVTNPDVDMFDR 180

Query: 181 EKVFIETVLKPLIERLPKLKVVMEHITTADAAKFVLSCEHALLAATVTPQHLLLNRNSLF 240
           EKVFIETVLKPLIERLPKLKVVMEHITTA+AAKFVLSCEHALLAATVTPQHLLLNRNSLF
Sbjct: 181 EKVFIETVLKPLIERLPKLKVVMEHITTANAAKFVLSCEHALLAATVTPQHLLLNRNSLF 240

Query: 241 QGGLQPHNYCLPVLKRETHRQAIVSAVTSGGKKFFLGTDSAPHERKRKESSCGCAGIYSA 300
           QGGLQPHNYCLPVLKRETHRQAIVSAVTSG KKFFLGTDSAPHER+RKESSCGCAGIYSA
Sbjct: 241 QGGLQPHNYCLPVLKRETHRQAIVSAVTSGSKKFFLGTDSAPHERRRKESSCGCAGIYSA 300

Query: 301 PIALSLYAKVFEEAGALDKLEAFTSFNGPDFYGLPRNTSKITLQKAPWQVPKSFSFSFGD 360
           PIALSLYAKVFEEAGALDKLEAFTSFNGPDFYGLPRNTSKITLQKAPWQVPKSF+F FGD
Sbjct: 301 PIALSLYAKVFEEAGALDKLEAFTSFNGPDFYGLPRNTSKITLQKAPWQVPKSFTFPFGD 360

Query: 361 IVPMFAGETLEWQPCFN 378
           IVPMFAGETLEWQPCFN
Sbjct: 361 IVPMFAGETLEWQPCFN 377

BLAST of CSPI03G38820 vs. ExPASy TrEMBL
Match: A0A5A7UFB1 (Dihydroorotase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold135G001630 PE=3 SV=1)

HSP 1 Score: 746.5 bits (1926), Expect = 5.4e-212
Identity = 369/378 (97.62%), Postives = 374/378 (98.94%), Query Frame = 0

Query: 1   MTKTLVLPFQPLKSPAQKFEGVKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKAVLPHSA 60
           MTKTLVLPFQPLKSPAQKFEGVKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKAVLPHSA
Sbjct: 88  MTKTLVLPFQPLKSPAQKFEGVKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKAVLPHSA 147

Query: 61  STFGRAIVMPNLKPPVTTTAAAVAYRDSILKALPSNSNFVPLMTLYLTDTMSPNEIKLAR 120
           STFGRAIVMPNLKPP+TTTAAAVAYRDSILKALPSNSNF+PLMTLYLTDTMSPNEIKLAR
Sbjct: 148 STFGRAIVMPNLKPPITTTAAAVAYRDSILKALPSNSNFIPLMTLYLTDTMSPNEIKLAR 207

Query: 121 KSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEQDMPLL-VHGEVTNSDVDMFD 180
           KSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEQDMPLL VHGEVTN DVDMFD
Sbjct: 208 KSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEQDMPLLVVHGEVTNPDVDMFD 267

Query: 181 REKVFIETVLKPLIERLPKLKVVMEHITTADAAKFVLSCEHALLAATVTPQHLLLNRNSL 240
           REKVFIETVLKPLIERLPKLKVVMEHITTA+AAKFVLSCEHALLAATVTPQHLLLNRNSL
Sbjct: 268 REKVFIETVLKPLIERLPKLKVVMEHITTANAAKFVLSCEHALLAATVTPQHLLLNRNSL 327

Query: 241 FQGGLQPHNYCLPVLKRETHRQAIVSAVTSGGKKFFLGTDSAPHERKRKESSCGCAGIYS 300
           FQGGLQPHNYCLPVLKRETHRQAIVSAVTSG KKFFLGTDSAPHER+RKESSCGCAGIYS
Sbjct: 328 FQGGLQPHNYCLPVLKRETHRQAIVSAVTSGSKKFFLGTDSAPHERRRKESSCGCAGIYS 387

Query: 301 APIALSLYAKVFEEAGALDKLEAFTSFNGPDFYGLPRNTSKITLQKAPWQVPKSFSFSFG 360
           APIALSLYAKVFEEAGALDKLEAFTSFNGPDFYGLPRNTSKITLQKAPWQVPKSF+F FG
Sbjct: 388 APIALSLYAKVFEEAGALDKLEAFTSFNGPDFYGLPRNTSKITLQKAPWQVPKSFTFPFG 447

Query: 361 DIVPMFAGETLEWQPCFN 378
           DIVPMFAGETLEWQPCFN
Sbjct: 448 DIVPMFAGETLEWQPCFN 465

BLAST of CSPI03G38820 vs. ExPASy TrEMBL
Match: A0A6J1F3S4 (Dihydroorotase OS=Cucurbita moschata OX=3662 GN=LOC111442116 PE=3 SV=1)

HSP 1 Score: 721.8 bits (1862), Expect = 1.4e-204
Identity = 353/377 (93.63%), Postives = 365/377 (96.82%), Query Frame = 0

Query: 1   MTKTLVLPFQPLKSPAQKFEGVKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKAVLPHSA 60
           MTKTL LPFQPLKSP QKFEG+KFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKAVLPHSA
Sbjct: 1   MTKTLALPFQPLKSPVQKFEGLKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKAVLPHSA 60

Query: 61  STFGRAIVMPNLKPPVTTTAAAVAYRDSILKALPSNSNFVPLMTLYLTDTMSPNEIKLAR 120
           S FGRAIVMPNLKPP+TTTAAAVAYRDSIL+ALPSNSNF PLMTLYLTDTM+PNEIKLAR
Sbjct: 61  SNFGRAIVMPNLKPPITTTAAAVAYRDSILEALPSNSNFTPLMTLYLTDTMNPNEIKLAR 120

Query: 121 KSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEQDMPLLVHGEVTNSDVDMFDR 180
           +SGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEQDMPLLVHGEVTN DVD+FDR
Sbjct: 121 RSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEQDMPLLVHGEVTNPDVDVFDR 180

Query: 181 EKVFIETVLKPLIERLPKLKVVMEHITTADAAKFVLSCEHALLAATVTPQHLLLNRNSLF 240
           EKVFIETVLKPLIERLPKLKVVMEHITTADAAKFV SCE+ LLAATVTPQHLLLNRNSLF
Sbjct: 181 EKVFIETVLKPLIERLPKLKVVMEHITTADAAKFVQSCENGLLAATVTPQHLLLNRNSLF 240

Query: 241 QGGLQPHNYCLPVLKRETHRQAIVSAVTSGGKKFFLGTDSAPHERKRKESSCGCAGIYSA 300
           QGGLQPHNYCLPVLKRETHRQAIVSAVTSG KKFFLGTDSAPH+R+RKE +CGCAGIYSA
Sbjct: 241 QGGLQPHNYCLPVLKRETHRQAIVSAVTSGSKKFFLGTDSAPHDRRRKECACGCAGIYSA 300

Query: 301 PIALSLYAKVFEEAGALDKLEAFTSFNGPDFYGLPRNTSKITLQKAPWQVPKSFSFSFGD 360
           PIALSLYAKVFEEA ALDKLEAFTSFNGPDFYGLPRNTSKITLQKAPW+VP+SFSF FGD
Sbjct: 301 PIALSLYAKVFEEACALDKLEAFTSFNGPDFYGLPRNTSKITLQKAPWKVPESFSFPFGD 360

Query: 361 IVPMFAGETLEWQPCFN 378
           IVPMFAGETLEWQPC N
Sbjct: 361 IVPMFAGETLEWQPCMN 377

BLAST of CSPI03G38820 vs. NCBI nr
Match: XP_004136697.2 (dihydroorotase, mitochondrial isoform X1 [Cucumis sativus] >KGN59552.1 hypothetical protein Csa_002648 [Cucumis sativus])

HSP 1 Score: 757.3 bits (1954), Expect = 6.3e-215
Identity = 374/377 (99.20%), Postives = 375/377 (99.47%), Query Frame = 0

Query: 1   MTKTLVLPFQPLKSPAQKFEGVKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKAVLPHSA 60
           MTKTLVLPFQPLKSP QKFEGVKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKAVLPHSA
Sbjct: 1   MTKTLVLPFQPLKSPGQKFEGVKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKAVLPHSA 60

Query: 61  STFGRAIVMPNLKPPVTTTAAAVAYRDSILKALPSNSNFVPLMTLYLTDTMSPNEIKLAR 120
           STFGRAIVMPNLKPPVTTTAAAVAYRDSILKALPSNSNFVPLMTLYLTDTMSPNEIKLAR
Sbjct: 61  STFGRAIVMPNLKPPVTTTAAAVAYRDSILKALPSNSNFVPLMTLYLTDTMSPNEIKLAR 120

Query: 121 KSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEQDMPLLVHGEVTNSDVDMFDR 180
           KSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEQDMPLLVHGEVTNSDVDMFDR
Sbjct: 121 KSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEQDMPLLVHGEVTNSDVDMFDR 180

Query: 181 EKVFIETVLKPLIERLPKLKVVMEHITTADAAKFVLSCEHALLAATVTPQHLLLNRNSLF 240
           EKVFIETVLKPLIERLPKLKVVMEHITTADAAKFVLSCEHALLAATVTPQHLLLNRNSLF
Sbjct: 181 EKVFIETVLKPLIERLPKLKVVMEHITTADAAKFVLSCEHALLAATVTPQHLLLNRNSLF 240

Query: 241 QGGLQPHNYCLPVLKRETHRQAIVSAVTSGGKKFFLGTDSAPHERKRKESSCGCAGIYSA 300
           QGGLQPHNYCLPVLKRETHRQAIVSAVTSG KKFFLGTDSAPHERKRKESSCGCAGIYSA
Sbjct: 241 QGGLQPHNYCLPVLKRETHRQAIVSAVTSGSKKFFLGTDSAPHERKRKESSCGCAGIYSA 300

Query: 301 PIALSLYAKVFEEAGALDKLEAFTSFNGPDFYGLPRNTSKITLQKAPWQVPKSFSFSFGD 360
           PIALSLYAKVFEEAGALDKLE+FTSFNGPDFYGLPRNTSKITLQKAPWQVPKSFSFSFGD
Sbjct: 301 PIALSLYAKVFEEAGALDKLESFTSFNGPDFYGLPRNTSKITLQKAPWQVPKSFSFSFGD 360

Query: 361 IVPMFAGETLEWQPCFN 378
           IVPMFAGETLEWQPCFN
Sbjct: 361 IVPMFAGETLEWQPCFN 377

BLAST of CSPI03G38820 vs. NCBI nr
Match: TYK25626.1 (dihydroorotase [Cucumis melo var. makuwa])

HSP 1 Score: 751.1 bits (1938), Expect = 4.5e-213
Identity = 369/377 (97.88%), Postives = 374/377 (99.20%), Query Frame = 0

Query: 1   MTKTLVLPFQPLKSPAQKFEGVKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKAVLPHSA 60
           MTKTLVLPFQPLKSPAQKFEGVKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKAVLPHSA
Sbjct: 88  MTKTLVLPFQPLKSPAQKFEGVKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKAVLPHSA 147

Query: 61  STFGRAIVMPNLKPPVTTTAAAVAYRDSILKALPSNSNFVPLMTLYLTDTMSPNEIKLAR 120
           STFGRAIVMPNLKPP+TTTAAAVAYRDSILKALPSNSNF+PLMTLYLTDTMSPNEIKLAR
Sbjct: 148 STFGRAIVMPNLKPPITTTAAAVAYRDSILKALPSNSNFIPLMTLYLTDTMSPNEIKLAR 207

Query: 121 KSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEQDMPLLVHGEVTNSDVDMFDR 180
           KSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEQDMPLLVHGEVTN DVDMFDR
Sbjct: 208 KSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEQDMPLLVHGEVTNPDVDMFDR 267

Query: 181 EKVFIETVLKPLIERLPKLKVVMEHITTADAAKFVLSCEHALLAATVTPQHLLLNRNSLF 240
           EKVFIETVLKPLIERLPKLKVVMEHITTA+AAKFVLSCEHALLAATVTPQHLLLNRNSLF
Sbjct: 268 EKVFIETVLKPLIERLPKLKVVMEHITTANAAKFVLSCEHALLAATVTPQHLLLNRNSLF 327

Query: 241 QGGLQPHNYCLPVLKRETHRQAIVSAVTSGGKKFFLGTDSAPHERKRKESSCGCAGIYSA 300
           QGGLQPHNYCLPVLKRETHRQAIVSAVTSG KKFFLGTDSAPHER+RKESSCGCAGIYSA
Sbjct: 328 QGGLQPHNYCLPVLKRETHRQAIVSAVTSGSKKFFLGTDSAPHERRRKESSCGCAGIYSA 387

Query: 301 PIALSLYAKVFEEAGALDKLEAFTSFNGPDFYGLPRNTSKITLQKAPWQVPKSFSFSFGD 360
           PIALSLYAKVFEEAGALDKLEAFTSFNGPDFYGLPRNTSKITLQKAPWQVPKSF+F FGD
Sbjct: 388 PIALSLYAKVFEEAGALDKLEAFTSFNGPDFYGLPRNTSKITLQKAPWQVPKSFTFPFGD 447

Query: 361 IVPMFAGETLEWQPCFN 378
           IVPMFAGETLEWQPCFN
Sbjct: 448 IVPMFAGETLEWQPCFN 464

BLAST of CSPI03G38820 vs. NCBI nr
Match: XP_008443394.1 (PREDICTED: dihydroorotase, mitochondrial isoform X1 [Cucumis melo])

HSP 1 Score: 751.1 bits (1938), Expect = 4.5e-213
Identity = 369/377 (97.88%), Postives = 374/377 (99.20%), Query Frame = 0

Query: 1   MTKTLVLPFQPLKSPAQKFEGVKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKAVLPHSA 60
           MTKTLVLPFQPLKSPAQKFEGVKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKAVLPHSA
Sbjct: 1   MTKTLVLPFQPLKSPAQKFEGVKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKAVLPHSA 60

Query: 61  STFGRAIVMPNLKPPVTTTAAAVAYRDSILKALPSNSNFVPLMTLYLTDTMSPNEIKLAR 120
           STFGRAIVMPNLKPP+TTTAAAVAYRDSILKALPSNSNF+PLMTLYLTDTMSPNEIKLAR
Sbjct: 61  STFGRAIVMPNLKPPITTTAAAVAYRDSILKALPSNSNFIPLMTLYLTDTMSPNEIKLAR 120

Query: 121 KSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEQDMPLLVHGEVTNSDVDMFDR 180
           KSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEQDMPLLVHGEVTN DVDMFDR
Sbjct: 121 KSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEQDMPLLVHGEVTNPDVDMFDR 180

Query: 181 EKVFIETVLKPLIERLPKLKVVMEHITTADAAKFVLSCEHALLAATVTPQHLLLNRNSLF 240
           EKVFIETVLKPLIERLPKLKVVMEHITTA+AAKFVLSCEHALLAATVTPQHLLLNRNSLF
Sbjct: 181 EKVFIETVLKPLIERLPKLKVVMEHITTANAAKFVLSCEHALLAATVTPQHLLLNRNSLF 240

Query: 241 QGGLQPHNYCLPVLKRETHRQAIVSAVTSGGKKFFLGTDSAPHERKRKESSCGCAGIYSA 300
           QGGLQPHNYCLPVLKRETHRQAIVSAVTSG KKFFLGTDSAPHER+RKESSCGCAGIYSA
Sbjct: 241 QGGLQPHNYCLPVLKRETHRQAIVSAVTSGSKKFFLGTDSAPHERRRKESSCGCAGIYSA 300

Query: 301 PIALSLYAKVFEEAGALDKLEAFTSFNGPDFYGLPRNTSKITLQKAPWQVPKSFSFSFGD 360
           PIALSLYAKVFEEAGALDKLEAFTSFNGPDFYGLPRNTSKITLQKAPWQVPKSF+F FGD
Sbjct: 301 PIALSLYAKVFEEAGALDKLEAFTSFNGPDFYGLPRNTSKITLQKAPWQVPKSFTFPFGD 360

Query: 361 IVPMFAGETLEWQPCFN 378
           IVPMFAGETLEWQPCFN
Sbjct: 361 IVPMFAGETLEWQPCFN 377

BLAST of CSPI03G38820 vs. NCBI nr
Match: KAA0053778.1 (dihydroorotase [Cucumis melo var. makuwa])

HSP 1 Score: 746.5 bits (1926), Expect = 1.1e-211
Identity = 369/378 (97.62%), Postives = 374/378 (98.94%), Query Frame = 0

Query: 1   MTKTLVLPFQPLKSPAQKFEGVKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKAVLPHSA 60
           MTKTLVLPFQPLKSPAQKFEGVKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKAVLPHSA
Sbjct: 88  MTKTLVLPFQPLKSPAQKFEGVKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKAVLPHSA 147

Query: 61  STFGRAIVMPNLKPPVTTTAAAVAYRDSILKALPSNSNFVPLMTLYLTDTMSPNEIKLAR 120
           STFGRAIVMPNLKPP+TTTAAAVAYRDSILKALPSNSNF+PLMTLYLTDTMSPNEIKLAR
Sbjct: 148 STFGRAIVMPNLKPPITTTAAAVAYRDSILKALPSNSNFIPLMTLYLTDTMSPNEIKLAR 207

Query: 121 KSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEQDMPLL-VHGEVTNSDVDMFD 180
           KSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEQDMPLL VHGEVTN DVDMFD
Sbjct: 208 KSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEQDMPLLVVHGEVTNPDVDMFD 267

Query: 181 REKVFIETVLKPLIERLPKLKVVMEHITTADAAKFVLSCEHALLAATVTPQHLLLNRNSL 240
           REKVFIETVLKPLIERLPKLKVVMEHITTA+AAKFVLSCEHALLAATVTPQHLLLNRNSL
Sbjct: 268 REKVFIETVLKPLIERLPKLKVVMEHITTANAAKFVLSCEHALLAATVTPQHLLLNRNSL 327

Query: 241 FQGGLQPHNYCLPVLKRETHRQAIVSAVTSGGKKFFLGTDSAPHERKRKESSCGCAGIYS 300
           FQGGLQPHNYCLPVLKRETHRQAIVSAVTSG KKFFLGTDSAPHER+RKESSCGCAGIYS
Sbjct: 328 FQGGLQPHNYCLPVLKRETHRQAIVSAVTSGSKKFFLGTDSAPHERRRKESSCGCAGIYS 387

Query: 301 APIALSLYAKVFEEAGALDKLEAFTSFNGPDFYGLPRNTSKITLQKAPWQVPKSFSFSFG 360
           APIALSLYAKVFEEAGALDKLEAFTSFNGPDFYGLPRNTSKITLQKAPWQVPKSF+F FG
Sbjct: 388 APIALSLYAKVFEEAGALDKLEAFTSFNGPDFYGLPRNTSKITLQKAPWQVPKSFTFPFG 447

Query: 361 DIVPMFAGETLEWQPCFN 378
           DIVPMFAGETLEWQPCFN
Sbjct: 448 DIVPMFAGETLEWQPCFN 465

BLAST of CSPI03G38820 vs. NCBI nr
Match: XP_038897131.1 (dihydroorotase, mitochondrial-like isoform X1 [Benincasa hispida])

HSP 1 Score: 736.9 bits (1901), Expect = 8.9e-209
Identity = 362/377 (96.02%), Postives = 366/377 (97.08%), Query Frame = 0

Query: 1   MTKTLVLPFQPLKSPAQKFEGVKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKAVLPHSA 60
           MTKTL LPFQPLKSP QKFEG+KFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKAVLPHSA
Sbjct: 1   MTKTLGLPFQPLKSPVQKFEGLKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKAVLPHSA 60

Query: 61  STFGRAIVMPNLKPPVTTTAAAVAYRDSILKALPSNSNFVPLMTLYLTDTMSPNEIKLAR 120
           S FGRAIVMPNLKPP+TTTAAAVAYRDSILKALPSNSNFVPLMTLYLTDTMSPNEIKLAR
Sbjct: 61  SNFGRAIVMPNLKPPITTTAAAVAYRDSILKALPSNSNFVPLMTLYLTDTMSPNEIKLAR 120

Query: 121 KSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEQDMPLLVHGEVTNSDVDMFDR 180
           +SGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEQDMPLLVHGEVTN DVDMFDR
Sbjct: 121 RSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEQDMPLLVHGEVTNPDVDMFDR 180

Query: 181 EKVFIETVLKPLIERLPKLKVVMEHITTADAAKFVLSCEHALLAATVTPQHLLLNRNSLF 240
           EKVFIETVLKPLIERLPKLKVVMEHITTADAAKFV SCEH LLAATVTPQHLLLNRNSLF
Sbjct: 181 EKVFIETVLKPLIERLPKLKVVMEHITTADAAKFVQSCEHGLLAATVTPQHLLLNRNSLF 240

Query: 241 QGGLQPHNYCLPVLKRETHRQAIVSAVTSGGKKFFLGTDSAPHERKRKESSCGCAGIYSA 300
           QGGLQPHNYCLPVLKRETHRQAIVSAVTSG KKFFLGTDSAPHER+RKE SCGCAGIYSA
Sbjct: 241 QGGLQPHNYCLPVLKRETHRQAIVSAVTSGSKKFFLGTDSAPHERRRKECSCGCAGIYSA 300

Query: 301 PIALSLYAKVFEEAGALDKLEAFTSFNGPDFYGLPRNTSKITLQKAPWQVPKSFSFSFGD 360
           PIALSLYAKVFEE GALDKLEAFTSFNGPDFYGLPRNTSKITL KAPWQVPKSFSF FGD
Sbjct: 301 PIALSLYAKVFEEVGALDKLEAFTSFNGPDFYGLPRNTSKITLHKAPWQVPKSFSFPFGD 360

Query: 361 IVPMFAGETLEWQPCFN 378
           IVPMFAGETLEWQPCFN
Sbjct: 361 IVPMFAGETLEWQPCFN 377

BLAST of CSPI03G38820 vs. TAIR 10
Match: AT4G22930.1 (pyrimidin 4 )

HSP 1 Score: 599.4 bits (1544), Expect = 2.1e-171
Identity = 292/375 (77.87%), Postives = 329/375 (87.73%), Query Frame = 0

Query: 1   MTKTLVLPFQPLKSPAQKFE-GVKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKAVLPHS 60
           M KTLV P+    S   KF+   + ++ +  +M+LT+TQPDDWHLHLRDGDLL AV+PHS
Sbjct: 1   MIKTLVSPYSGFGSQKLKFDRSSEKVKTRAVRMELTITQPDDWHLHLRDGDLLHAVVPHS 60

Query: 61  ASTFGRAIVMPNLKPPVTTTAAAVAYRDSILKALPSNSNFVPLMTLYLTDTMSPNEIKLA 120
           AS F RAIVMPNLKPPVT+TAAA+ YR  I+KALPS S+F PLMTLYLTD   P EI+LA
Sbjct: 61  ASNFKRAIVMPNLKPPVTSTAAAIIYRKFIMKALPSESSFDPLMTLYLTDKTLPEEIRLA 120

Query: 121 RKSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEQDMPLLVHGEVTNSDVDMFD 180
           R+SG VYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMV+Q+MPLLVHGEVT+  +D+FD
Sbjct: 121 RESGVVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVKQNMPLLVHGEVTDPSIDVFD 180

Query: 181 REKVFIETVLKPLIERLPKLKVVMEHITTADAAKFVLSCEHALLAATVTPQHLLLNRNSL 240
           REK+FIETVL+PLI+RLP+LKVVMEHITT DA  FV SC+   + ATVTPQHLLLNRN+L
Sbjct: 181 REKIFIETVLQPLIQRLPQLKVVMEHITTMDAVNFVESCKEGSVGATVTPQHLLLNRNAL 240

Query: 241 FQGGLQPHNYCLPVLKRETHRQAIVSAVTSGGKKFFLGTDSAPHERKRKESSCGCAGIYS 300
           FQGGLQPHNYCLPVLKRE HR+AIV AVTSG KKFFLGTDSAPHER RKESSCGCAGIYS
Sbjct: 241 FQGGLQPHNYCLPVLKREIHREAIVKAVTSGSKKFFLGTDSAPHERSRKESSCGCAGIYS 300

Query: 301 APIALSLYAKVFEEAGALDKLEAFTSFNGPDFYGLPRNTSKITLQKAPWQVPKSFSFSFG 360
           APIALSLYAKVF+EAGALDKLEAFTSFNGPDFYGLPRN+SKITL+K+PW+VP  F+F FG
Sbjct: 301 APIALSLYAKVFDEAGALDKLEAFTSFNGPDFYGLPRNSSKITLKKSPWKVPDVFNFPFG 360

Query: 361 DIVPMFAGETLEWQP 375
           +IVPMFAGETL+WQP
Sbjct: 361 EIVPMFAGETLQWQP 375

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O049042.9e-17077.87Dihydroorotase, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=PYR4 PE=1 SV=1[more]
Q0JJD11.3e-16278.84Dihydroorotase, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=PYRC P... [more]
B1XI962.0e-12665.69Dihydroorotase OS=Synechococcus sp. (strain ATCC 27264 / PCC 7002 / PR-6) OX=320... [more]
C1D6723.5e-12365.40Dihydroorotase OS=Laribacter hongkongensis (strain HLHK9) OX=557598 GN=pyrC PE=3... [more]
B0CF788.6e-12263.64Dihydroorotase OS=Acaryochloris marina (strain MBIC 11017) OX=329726 GN=pyrC PE=... [more]
Match NameE-valueIdentityDescription
A0A0A0LG113.1e-21599.20Dihydroorotase OS=Cucumis sativus OX=3659 GN=Csa_3G824970 PE=3 SV=1[more]
A0A5D3DPM62.2e-21397.88Dihydroorotase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G00786... [more]
A0A1S3B7W22.2e-21397.88Dihydroorotase OS=Cucumis melo OX=3656 GN=LOC103486989 PE=3 SV=1[more]
A0A5A7UFB15.4e-21297.62Dihydroorotase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold135G00163... [more]
A0A6J1F3S41.4e-20493.63Dihydroorotase OS=Cucurbita moschata OX=3662 GN=LOC111442116 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_004136697.26.3e-21599.20dihydroorotase, mitochondrial isoform X1 [Cucumis sativus] >KGN59552.1 hypotheti... [more]
TYK25626.14.5e-21397.88dihydroorotase [Cucumis melo var. makuwa][more]
XP_008443394.14.5e-21397.88PREDICTED: dihydroorotase, mitochondrial isoform X1 [Cucumis melo][more]
KAA0053778.11.1e-21197.62dihydroorotase [Cucumis melo var. makuwa][more]
XP_038897131.18.9e-20996.02dihydroorotase, mitochondrial-like isoform X1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT4G22930.12.1e-17177.87pyrimidin 4 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006680Amidohydrolase-relatedPFAMPF01979Amidohydro_1coord: 10..319
e-value: 3.5E-27
score: 95.6
IPR004721Dihydroorotase homodimeric typeTIGRFAMTIGR00856TIGR00856coord: 3..342
e-value: 1.7E-145
score: 482.3
IPR004721Dihydroorotase homodimeric typePIRSFPIRSF001237Dihydroorotasecoord: 1..345
e-value: 5.8E-146
score: 483.8
IPR004721Dihydroorotase homodimeric typePANTHERPTHR43137DIHYDROOROTASEcoord: 1..344
IPR004721Dihydroorotase homodimeric typeHAMAPMF_00219PyrC_classIIcoord: 1..344
score: 47.12632
IPR004721Dihydroorotase homodimeric typeCDDcd01294DHOasecoord: 3..341
e-value: 0.0
score: 568.452
NoneNo IPR availableGENE3D3.20.20.140coord: 1..343
e-value: 1.2E-126
score: 424.5
IPR002195Dihydroorotase, conserved sitePROSITEPS00483DIHYDROOROTASE_2coord: 246..257
IPR002195Dihydroorotase, conserved sitePROSITEPS00482DIHYDROOROTASE_1coord: 10..18
IPR032466Metal-dependent hydrolaseSUPERFAMILY51556Metallo-dependent hydrolasescoord: 2..342

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI03G38820.1CSPI03G38820.1mRNA
CSPI03G38820.2CSPI03G38820.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006207 'de novo' pyrimidine nucleobase biosynthetic process
biological_process GO:0044205 'de novo' UMP biosynthetic process
biological_process GO:0019856 pyrimidine nucleobase biosynthetic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0004151 dihydroorotase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016787 hydrolase activity
molecular_function GO:0016812 hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amides