CSPI03G37700 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI03G37700
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	3'-5' exonuclease domain-containing protein
Location	Chr3: 32650808 .. 32651721 (-)
RNA-Seq Expression	CSPI03G37700
Synteny	CSPI03G37700

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ACCTGCTTTAGTCTTTGAAGTTCTCAGCAATGGCGATCACCATTGTTGACCATGAAGTTCCCTCAGATTCCCACAATTACTTCGACATAACCTTCGACACTGACGAGCCAATTCTCACTCTTCTCACCACTTCACCATCCATGGTAGACGGTTGGATTTCACAAACCCTCGCCATTCAAACTCCACCTCTCCTCGTCGGCCTCGACATCGAATGGCGCCCTAACAATCGCTCCTACGACAATCCTGTTGCCACCTTACAACTCTGCATTGGCCGCCGCTGCCTGATCTTGCAATTGCTTCACATGCCAGAGATCCCTAAATCTCTGTTCGAGTTTTTGGAAAACGAATCATTCACATTCGTAGGAGTGGGAATCGACGAAGATGCGGAAAAGCTAAACTGTGACTACGGATTGAAAGTGGGGAAGAGAATGGATCTGAGGGATTTGGCTGAGAGTGTAACGGGAAGAGGAGAATTGAAGAATGCGGGGTTGAAGAGATTGGGGAAAGAAGTGTTGGGGAAAGAGATTCATAAACCGAAGAGTGTGACGATGAGTAGATGGGATCAAGAATGGCTTACGCTTAATCAGGTGAAGTATGCTTGTATTGATGCGTTTTTCTCGTTTGAGATTGGAAAGTTTTTGCAATCTGCATCCTATTGAAAGGGGAATTTGGGGATTTTTCTTTCTTCTTTTTTTAAGGTTTAAGGGTTATTTGTTTGTTTTGGCTGCTTGATGGATGTGTTCTTAGCTTGGCTATGGTGTTTTTCTTTGCAAATCAAAACTTCGTGGAAATTTGGATGGATTGGATGATAATTTGCTCTTTACTTTACCAAACTTGGATCTCAATTTTAGAATTTACTGTGGTGGAGATCAAATGGTTTGTGATTAGTATGTAAACTATATTGAACATGGATG

mRNA sequence

ACCTGCTTTAGTCTTTGAAGTTCTCAGCAATGGCGATCACCATTGTTGACCATGAAGTTCCCTCAGATTCCCACAATTACTTCGACATAACCTTCGACACTGACGAGCCAATTCTCACTCTTCTCACCACTTCACCATCCATGGTAGACGGTTGGATTTCACAAACCCTCGCCATTCAAACTCCACCTCTCCTCGTCGGCCTCGACATCGAATGGCGCCCTAACAATCGCTCCTACGACAATCCTGTTGCCACCTTACAACTCTGCATTGGCCGCCGCTGCCTGATCTTGCAATTGCTTCACATGCCAGAGATCCCTAAATCTCTGTTCGAGTTTTTGGAAAACGAATCATTCACATTCGTAGGAGTGGGAATCGACGAAGATGCGGAAAAGCTAAACTGTGACTACGGATTGAAAGTGGGGAAGAGAATGGATCTGAGGGATTTGGCTGAGAGTGTAACGGGAAGAGGAGAATTGAAGAATGCGGGGTTGAAGAGATTGGGGAAAGAAGTGTTGGGGAAAGAGATTCATAAACCGAAGAGTGTGACGATGAGTAGATGGGATCAAGAATGGCTTACGCTTAATCAGGTGAAGTATGCTTGTATTGATGCGTTTTTCTCGTTTGAGATTGGAAAGTTTTTGCAATCTGCATCCTATTGAAAGGGGAATTTGGGGATTTTTCTTTCTTCTTTTTTTAAGGTTTAAGGGTTATTTGTTTGTTTTGGCTGCTTGATGGATGTGTTCTTAGCTTGGCTATGGTGTTTTTCTTTGCAAATCAAAACTTCGTGGAAATTTGGATGGATTGGATGATAATTTGCTCTTTACTTTACCAAACTTGGATCTCAATTTTAGAATTTACTGTGGTGGAGATCAAATGGTTTGTGATTAGTATGTAAACTATATTGAACATGGATG

Coding sequence (CDS)

ATGGCGATCACCATTGTTGACCATGAAGTTCCCTCAGATTCCCACAATTACTTCGACATAACCTTCGACACTGACGAGCCAATTCTCACTCTTCTCACCACTTCACCATCCATGGTAGACGGTTGGATTTCACAAACCCTCGCCATTCAAACTCCACCTCTCCTCGTCGGCCTCGACATCGAATGGCGCCCTAACAATCGCTCCTACGACAATCCTGTTGCCACCTTACAACTCTGCATTGGCCGCCGCTGCCTGATCTTGCAATTGCTTCACATGCCAGAGATCCCTAAATCTCTGTTCGAGTTTTTGGAAAACGAATCATTCACATTCGTAGGAGTGGGAATCGACGAAGATGCGGAAAAGCTAAACTGTGACTACGGATTGAAAGTGGGGAAGAGAATGGATCTGAGGGATTTGGCTGAGAGTGTAACGGGAAGAGGAGAATTGAAGAATGCGGGGTTGAAGAGATTGGGGAAAGAAGTGTTGGGGAAAGAGATTCATAAACCGAAGAGTGTGACGATGAGTAGATGGGATCAAGAATGGCTTACGCTTAATCAGGTGAAGTATGCTTGTATTGATGCGTTTTTCTCGTTTGAGATTGGAAAGTTTTTGCAATCTGCATCCTATTGA

Protein sequence

MAITIVDHEVPSDSHNYFDITFDTDEPILTLLTTSPSMVDGWISQTLAIQTPPLLVGLDIEWRPNNRSYDNPVATLQLCIGRRCLILQLLHMPEIPKSLFEFLENESFTFVGVGIDEDAEKLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQEWLTLNQVKYACIDAFFSFEIGKFLQSASY*

Homology

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BLAST of CSPI03G37700 vs. ExPASy Swiss-Prot
Match: Q84LH3 (Werner Syndrome-like exonuclease OS=Arabidopsis thaliana OX=3702 GN=WEX PE=1 SV=1)

HSP 1 Score: 96.3 bits (238), Expect = 4.4e-19
Identity = 59/153 (38.56%), Postives = 89/153 (58.17%), Query Frame = 0


Query: 56  VGLDIEWRPNNRSYDNP--VATLQLCI-GRRCLILQLLHMPEIPKSLFEFLENESFTFVG 115
           VGLDIEWRP+ R    P  VAT+Q+C+    C ++ + H   IP+SL   +E+ +   VG
Sbjct: 130 VGLDIEWRPSFRKGVLPGKVATVQICVDSNYCDVMHIFH-SGIPQSLQHLIEDSTLVKVG 189

Query: 116 VGIDEDAEKLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSV 175
           +GID D+ KL  DYG+ +    DL DLA    G G+ K  GL  L + ++ KE+ KP  +
Sbjct: 190 IGIDGDSVKLFHDYGVSIKDVEDLSDLANQKIG-GD-KKWGLASLTETLVCKELLKPNRI 249

Query: 176 TMSRWDQEWLTLNQVKYACIDAFFSFEIGKFLQ 206
            +  W+   L+  Q++YA  DA+ S+ + K L+
Sbjct: 250 RLGNWEFYPLSKQQLQYAATDAYASWHLYKVLK 279

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BLAST of CSPI03G37700 vs. ExPASy Swiss-Prot
Match: Q14191 (Werner syndrome ATP-dependent helicase OS=Homo sapiens OX=9606 GN=WRN PE=1 SV=2)

HSP 1 Score: 83.6 bits (205), Expect = 3.0e-15
Identity = 54/153 (35.29%), Postives = 80/153 (52.29%), Query Frame = 0


Query: 55  LVGLDIEWRP-NNRSYDNPVATLQLCIGR-RCLILQLLHMPEIPKSLFEFLENESFTFVG 114
           +VG D+EW P  NR     VA +QLC+   +C +  +  M   P+ L   LEN++    G
Sbjct: 78  VVGFDMEWPPLYNRGKLGKVALIQLCVSESKCYLFHVSSMSVFPQGLKMLLENKAVKKAG 137

Query: 115 VGIDEDAEKLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSV 174
           VGI+ D  KL  D+ +K+   ++L D+A       E     L  L K +LGK++ K KS+
Sbjct: 138 VGIEGDQWKLLRDFDIKLKNFVELTDVANKKLKCTE--TWSLNSLVKHLLGKQLLKDKSI 197

Query: 175 TMSRWDQEWLTLNQVKYACIDAFFSFEIGKFLQ 206
             S W +  LT +Q  YA  DA+  F I + L+
Sbjct: 198 RCSNWSKFPLTEDQKLYAATDAYAGFIIYRNLE 228

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BLAST of CSPI03G37700 vs. ExPASy Swiss-Prot
Match: Q9VGN7 (Exonuclease 3'-5' domain-containing protein 2 OS=Drosophila melanogaster OX=7227 GN=Exd2 PE=2 SV=2)

HSP 1 Score: 80.1 bits (196), Expect = 3.3e-14
Identity = 55/147 (37.41%), Postives = 74/147 (50.34%), Query Frame = 0


Query: 55  LVGLDIEWRPNNRSYDNPVATLQLCIGR-RCLILQLLHMPEIPKSLFEFLENESFTFVGV 114
           ++G D EW     S   PVA LQL   R  C + +L HM +IP+ L E LE++S   VGV
Sbjct: 79  VLGFDCEWITVGGS-RRPVALLQLSSHRGLCALFRLCHMKQIPQDLRELLEDDSVIKVGV 138

Query: 115 GIDEDAEKLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSVT 174
              EDA KL+ DYG+ V   +DLR L      + E    GL +L K  L   + K   + 
Sbjct: 139 APQEDAMKLSHDYGVGVASTLDLRFLCVMAGHKPE----GLGKLSKTHLNYTLDKHWRLA 198

Query: 175 MSRWDQEWLTLNQVKYACIDAFFSFEI 201
            S W+ + L   Q+ YA  DA  +  I
Sbjct: 199 CSNWEAKTLEPKQLDYAANDALMAVAI 220

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BLAST of CSPI03G37700 vs. ExPASy Swiss-Prot
Match: O09053 (Werner syndrome ATP-dependent helicase homolog OS=Mus musculus OX=10090 GN=Wrn PE=1 SV=3)

HSP 1 Score: 80.1 bits (196), Expect = 3.3e-14
Identity = 53/146 (36.30%), Postives = 75/146 (51.37%), Query Frame = 0


Query: 55  LVGLDIEW----RPNNRSYDNPVATLQLCIGR-RCLILQLLHMPEIPKSLFEFLENESFT 114
           +VG D+EW    +P  RS    VA +QLC+   +C +  +  M   P+ L   LEN+S  
Sbjct: 72  VVGFDMEWPPIYKPGKRS---RVAVIQLCVSESKCYLFHISSMSVFPQGLKMLLENKSIK 131

Query: 115 FVGVGIDEDAEKLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKP 174
             GVGI+ D  KL  D+ +K+   ++L D+A       E     L  L K VLGK++ K 
Sbjct: 132 KAGVGIEGDQWKLLRDFDVKLESFVELTDVANEKLKCAE--TWSLNGLVKHVLGKQLLKD 191

Query: 175 KSVTMSRWDQEWLTLNQVKYACIDAF 196
           KS+  S W    LT +Q  YA  DA+
Sbjct: 192 KSIRCSNWSNFPLTEDQKLYAATDAY 212

[Close]

BLAST of CSPI03G37700 vs. ExPASy Swiss-Prot
Match: O93530 (Werner syndrome ATP-dependent helicase homolog OS=Xenopus laevis OX=8355 GN=wrn PE=2 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 9.6e-14
Identity = 55/155 (35.48%), Postives = 79/155 (50.97%), Query Frame = 0


Query: 55  LVGLDIEWRP-NNRSYDNPVATLQLCIG-RRCLILQLLHMPEIPKSLFEFLENESFTFVG 114
           ++G DIEW P   +     VA +Q+C+  ++C +  +  M   PK L   LE+ES   VG
Sbjct: 72  VLGFDIEWPPVYTKGKTGKVALIQVCVSEKKCYLFHISPMAGFPKGLKRLLEDESVRKVG 131

Query: 115 VGIDEDAEKLNCDYGLKVGKRMDLRDLAESVTGRGE--LKNAGLKRLGKEVLGKEIHKPK 174
           VGI+ D  KL  DY LK+   ++L ++A       E    N  +K L KE    +++K K
Sbjct: 132 VGIEGDQWKLMSDYELKLKGFIELSEMANQKLRCKEKWTFNGLIKHLFKE----QLYKRK 191

Query: 175 SVTMSRWDQEWLTLNQVKYACIDAFFSFEIGKFLQ 206
           S   S WD   LT +Q  YA  DA+    I K L+
Sbjct: 192 SYRCSNWDIFLLTEDQKLYAATDAYAGLLIYKKLE 222

[Close]

BLAST of CSPI03G37700 vs. ExPASy TrEMBL
Match: A0A0A0LBN3 (3'-5' exonuclease domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G819870 PE=4 SV=1)

HSP 1 Score: 429.1 bits (1102), Expect = 1.1e-116
Identity = 209/209 (100.00%), Postives = 209/209 (100.00%), Query Frame = 0


Query: 1   MAITIVDHEVPSDSHNYFDITFDTDEPILTLLTTSPSMVDGWISQTLAIQTPPLLVGLDI 60
           MAITIVDHEVPSDSHNYFDITFDTDEPILTLLTTSPSMVDGWISQTLAIQTPPLLVGLDI
Sbjct: 1   MAITIVDHEVPSDSHNYFDITFDTDEPILTLLTTSPSMVDGWISQTLAIQTPPLLVGLDI 60

Query: 61  EWRPNNRSYDNPVATLQLCIGRRCLILQLLHMPEIPKSLFEFLENESFTFVGVGIDEDAE 120
           EWRPNNRSYDNPVATLQLCIGRRCLILQLLHMPEIPKSLFEFLENESFTFVGVGIDEDAE
Sbjct: 61  EWRPNNRSYDNPVATLQLCIGRRCLILQLLHMPEIPKSLFEFLENESFTFVGVGIDEDAE 120

Query: 121 KLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQE 180
           KLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQE
Sbjct: 121 KLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQE 180

Query: 181 WLTLNQVKYACIDAFFSFEIGKFLQSASY 210
           WLTLNQVKYACIDAFFSFEIGKFLQSASY
Sbjct: 181 WLTLNQVKYACIDAFFSFEIGKFLQSASY 209

[Close]

BLAST of CSPI03G37700 vs. ExPASy TrEMBL
Match: A0A5A7UI20 (Werner Syndrome-like exonuclease OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G006600 PE=4 SV=1)

HSP 1 Score: 414.5 bits (1064), Expect = 2.7e-112
Identity = 201/208 (96.63%), Postives = 204/208 (98.08%), Query Frame = 0


Query: 1   MAITIVDHEVPSDSHNYFDITFDTDEPILTLLTTSPSMVDGWISQTLAIQTPPLLVGLDI 60
           M+ITIVDHEVPSDSHNYFDITFD DEPILTLLTTSPSMVD WISQTL IQTPPLLVGLDI
Sbjct: 1   MSITIVDHEVPSDSHNYFDITFDNDEPILTLLTTSPSMVDDWISQTLTIQTPPLLVGLDI 60

Query: 61  EWRPNNRSYDNPVATLQLCIGRRCLILQLLHMPEIPKSLFEFLENESFTFVGVGIDEDAE 120
           EWRPNNRSYDNPVATLQLCIGRRCLILQLLH+PEIPKSLFEFLENESFTFVGVGIDEDAE
Sbjct: 61  EWRPNNRSYDNPVATLQLCIGRRCLILQLLHIPEIPKSLFEFLENESFTFVGVGIDEDAE 120

Query: 121 KLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQE 180
           KLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQE
Sbjct: 121 KLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQE 180

Query: 181 WLTLNQVKYACIDAFFSFEIGKFLQSAS 209
           WLTLNQVKYACIDAFFSFEIG+FLQS S
Sbjct: 181 WLTLNQVKYACIDAFFSFEIGRFLQSES 208

[Close]

BLAST of CSPI03G37700 vs. ExPASy TrEMBL
Match: E5GBP0 (3'-5' exonuclease OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 414.5 bits (1064), Expect = 2.7e-112
Identity = 201/208 (96.63%), Postives = 204/208 (98.08%), Query Frame = 0


Query: 1   MAITIVDHEVPSDSHNYFDITFDTDEPILTLLTTSPSMVDGWISQTLAIQTPPLLVGLDI 60
           M+ITIVDHEVPSDSHNYFDITFD DEPILTLLTTSPSMVD WISQTL IQTPPLLVGLDI
Sbjct: 1   MSITIVDHEVPSDSHNYFDITFDNDEPILTLLTTSPSMVDDWISQTLTIQTPPLLVGLDI 60

Query: 61  EWRPNNRSYDNPVATLQLCIGRRCLILQLLHMPEIPKSLFEFLENESFTFVGVGIDEDAE 120
           EWRPNNRSYDNPVATLQLCIGRRCLILQLLH+PEIPKSLFEFLENESFTFVGVGIDEDAE
Sbjct: 61  EWRPNNRSYDNPVATLQLCIGRRCLILQLLHIPEIPKSLFEFLENESFTFVGVGIDEDAE 120

Query: 121 KLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQE 180
           KLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQE
Sbjct: 121 KLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQE 180

Query: 181 WLTLNQVKYACIDAFFSFEIGKFLQSAS 209
           WLTLNQVKYACIDAFFSFEIG+FLQS S
Sbjct: 181 WLTLNQVKYACIDAFFSFEIGRFLQSES 208

[Close]

BLAST of CSPI03G37700 vs. ExPASy TrEMBL
Match: A0A1S3B738 (Werner Syndrome-like exonuclease OS=Cucumis melo OX=3656 GN=LOC103486879 PE=4 SV=1)

HSP 1 Score: 414.5 bits (1064), Expect = 2.7e-112
Identity = 201/208 (96.63%), Postives = 204/208 (98.08%), Query Frame = 0


Query: 1   MAITIVDHEVPSDSHNYFDITFDTDEPILTLLTTSPSMVDGWISQTLAIQTPPLLVGLDI 60
           M+ITIVDHEVPSDSHNYFDITFD DEPILTLLTTSPSMVD WISQTL IQTPPLLVGLDI
Sbjct: 1   MSITIVDHEVPSDSHNYFDITFDNDEPILTLLTTSPSMVDDWISQTLTIQTPPLLVGLDI 60

Query: 61  EWRPNNRSYDNPVATLQLCIGRRCLILQLLHMPEIPKSLFEFLENESFTFVGVGIDEDAE 120
           EWRPNNRSYDNPVATLQLCIGRRCLILQLLH+PEIPKSLFEFLENESFTFVGVGIDEDAE
Sbjct: 61  EWRPNNRSYDNPVATLQLCIGRRCLILQLLHIPEIPKSLFEFLENESFTFVGVGIDEDAE 120

Query: 121 KLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQE 180
           KLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQE
Sbjct: 121 KLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQE 180

Query: 181 WLTLNQVKYACIDAFFSFEIGKFLQSAS 209
           WLTLNQVKYACIDAFFSFEIG+FLQS S
Sbjct: 181 WLTLNQVKYACIDAFFSFEIGRFLQSES 208

[Close]

BLAST of CSPI03G37700 vs. ExPASy TrEMBL
Match: A0A6J1F975 (Werner Syndrome-like exonuclease OS=Cucurbita moschata OX=3662 GN=LOC111441964 PE=4 SV=1)

HSP 1 Score: 334.3 bits (856), Expect = 3.6e-88
Identity = 159/208 (76.44%), Postives = 182/208 (87.50%), Query Frame = 0


Query: 1   MAITIVDHEVPSDSHNYFDITFDTDEPILTLLTTSPSMVDGWISQTLAIQTPPLLVGLDI 60
           MA+TIVD E PS SHNYFD+TF++DEPILTLLT SP MVD WIS+TL  QT PL+VGLDI
Sbjct: 1   MALTIVDREDPSGSHNYFDVTFESDEPILTLLTISPLMVDDWISETLNTQTSPLIVGLDI 60

Query: 61  EWRPNNRSYDNPVATLQLCIGRRCLILQLLHMPEIPKSLFEFLENESFTFVGVGIDEDAE 120
           EWRPN RSY+NPVATLQLC GRRCLI+QL++ PE PKSL+EFLENES +FVGVGI+ DAE
Sbjct: 61  EWRPNIRSYENPVATLQLCTGRRCLIVQLIYAPEFPKSLYEFLENESISFVGVGIEGDAE 120

Query: 121 KLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQE 180
           KLNCDYGLKV K +DLR+LAESVTGR ELKNAGLK L KE+LGKEI KP+ VT+SRWDQE
Sbjct: 121 KLNCDYGLKVRKTVDLRNLAESVTGRRELKNAGLKSLAKEILGKEIRKPRGVTLSRWDQE 180

Query: 181 WLTLNQVKYACIDAFFSFEIGKFLQSAS 209
           WLT NQ+KYAC DAF S+EIG+ LQ++S
Sbjct: 181 WLTHNQIKYACFDAFVSYEIGRHLQASS 208

[Close]

BLAST of CSPI03G37700 vs. NCBI nr
Match: XP_011652174.1 (Werner Syndrome-like exonuclease [Cucumis sativus] >KGN59430.1 hypothetical protein Csa_001395 [Cucumis sativus])

HSP 1 Score: 429.1 bits (1102), Expect = 2.2e-116
Identity = 209/209 (100.00%), Postives = 209/209 (100.00%), Query Frame = 0


Query: 1   MAITIVDHEVPSDSHNYFDITFDTDEPILTLLTTSPSMVDGWISQTLAIQTPPLLVGLDI 60
           MAITIVDHEVPSDSHNYFDITFDTDEPILTLLTTSPSMVDGWISQTLAIQTPPLLVGLDI
Sbjct: 1   MAITIVDHEVPSDSHNYFDITFDTDEPILTLLTTSPSMVDGWISQTLAIQTPPLLVGLDI 60

Query: 61  EWRPNNRSYDNPVATLQLCIGRRCLILQLLHMPEIPKSLFEFLENESFTFVGVGIDEDAE 120
           EWRPNNRSYDNPVATLQLCIGRRCLILQLLHMPEIPKSLFEFLENESFTFVGVGIDEDAE
Sbjct: 61  EWRPNNRSYDNPVATLQLCIGRRCLILQLLHMPEIPKSLFEFLENESFTFVGVGIDEDAE 120

Query: 121 KLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQE 180
           KLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQE
Sbjct: 121 KLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQE 180

Query: 181 WLTLNQVKYACIDAFFSFEIGKFLQSASY 210
           WLTLNQVKYACIDAFFSFEIGKFLQSASY
Sbjct: 181 WLTLNQVKYACIDAFFSFEIGKFLQSASY 209

[Close]

BLAST of CSPI03G37700 vs. NCBI nr
Match: XP_008443244.1 (PREDICTED: Werner Syndrome-like exonuclease [Cucumis melo] >ADN33882.1 3'-5' exonuclease [Cucumis melo subsp. melo] >KAA0053896.1 Werner Syndrome-like exonuclease [Cucumis melo var. makuwa] >TYK25510.1 Werner Syndrome-like exonuclease [Cucumis melo var. makuwa])

HSP 1 Score: 414.5 bits (1064), Expect = 5.6e-112
Identity = 201/208 (96.63%), Postives = 204/208 (98.08%), Query Frame = 0


Query: 1   MAITIVDHEVPSDSHNYFDITFDTDEPILTLLTTSPSMVDGWISQTLAIQTPPLLVGLDI 60
           M+ITIVDHEVPSDSHNYFDITFD DEPILTLLTTSPSMVD WISQTL IQTPPLLVGLDI
Sbjct: 1   MSITIVDHEVPSDSHNYFDITFDNDEPILTLLTTSPSMVDDWISQTLTIQTPPLLVGLDI 60

Query: 61  EWRPNNRSYDNPVATLQLCIGRRCLILQLLHMPEIPKSLFEFLENESFTFVGVGIDEDAE 120
           EWRPNNRSYDNPVATLQLCIGRRCLILQLLH+PEIPKSLFEFLENESFTFVGVGIDEDAE
Sbjct: 61  EWRPNNRSYDNPVATLQLCIGRRCLILQLLHIPEIPKSLFEFLENESFTFVGVGIDEDAE 120

Query: 121 KLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQE 180
           KLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQE
Sbjct: 121 KLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQE 180

Query: 181 WLTLNQVKYACIDAFFSFEIGKFLQSAS 209
           WLTLNQVKYACIDAFFSFEIG+FLQS S
Sbjct: 181 WLTLNQVKYACIDAFFSFEIGRFLQSES 208

[Close]

BLAST of CSPI03G37700 vs. NCBI nr
Match: XP_038903327.1 (Werner Syndrome-like exonuclease isoform X2 [Benincasa hispida])

HSP 1 Score: 355.5 bits (911), Expect = 3.1e-94
Identity = 169/187 (90.37%), Postives = 181/187 (96.79%), Query Frame = 0


Query: 1   MAITIVDHEVPSDSHNYFDITFDTDEPILTLLTTSPSMVDGWISQTLAIQTPPLLVGLDI 60
           MAITIVDH+VPSDSHNYFDIT D+DEPILTLLT SPSMV+ WIS+TLA+QTPPL+VGLDI
Sbjct: 1   MAITIVDHQVPSDSHNYFDITLDSDEPILTLLTNSPSMVENWISETLALQTPPLMVGLDI 60

Query: 61  EWRPNNRSYDNPVATLQLCIGRRCLILQLLHMPEIPKSLFEFLENESFTFVGVGIDEDAE 120
           EWRPNNRSYDNPVATLQLC+GRRCLILQL+H PEIPKSLFEFLENES TFVGVGIDEDAE
Sbjct: 61  EWRPNNRSYDNPVATLQLCMGRRCLILQLIHTPEIPKSLFEFLENESLTFVGVGIDEDAE 120

Query: 121 KLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQE 180
           KLNCDYGLKVGKR+DLR+LAESVTGR +LKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQE
Sbjct: 121 KLNCDYGLKVGKRVDLRNLAESVTGRRDLKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQE 180

Query: 181 WLTLNQV 188
           WLTLNQ+
Sbjct: 181 WLTLNQM 187

[Close]

BLAST of CSPI03G37700 vs. NCBI nr
Match: XP_038903326.1 (Werner Syndrome-like exonuclease isoform X1 [Benincasa hispida])

HSP 1 Score: 354.8 bits (909), Expect = 5.3e-94
Identity = 169/186 (90.86%), Postives = 180/186 (96.77%), Query Frame = 0


Query: 1   MAITIVDHEVPSDSHNYFDITFDTDEPILTLLTTSPSMVDGWISQTLAIQTPPLLVGLDI 60
           MAITIVDH+VPSDSHNYFDIT D+DEPILTLLT SPSMV+ WIS+TLA+QTPPL+VGLDI
Sbjct: 1   MAITIVDHQVPSDSHNYFDITLDSDEPILTLLTNSPSMVENWISETLALQTPPLMVGLDI 60

Query: 61  EWRPNNRSYDNPVATLQLCIGRRCLILQLLHMPEIPKSLFEFLENESFTFVGVGIDEDAE 120
           EWRPNNRSYDNPVATLQLC+GRRCLILQL+H PEIPKSLFEFLENES TFVGVGIDEDAE
Sbjct: 61  EWRPNNRSYDNPVATLQLCMGRRCLILQLIHTPEIPKSLFEFLENESLTFVGVGIDEDAE 120

Query: 121 KLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQE 180
           KLNCDYGLKVGKR+DLR+LAESVTGR +LKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQE
Sbjct: 121 KLNCDYGLKVGKRVDLRNLAESVTGRRDLKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQE 180

Query: 181 WLTLNQ 187
           WLTLNQ
Sbjct: 181 WLTLNQ 186

[Close]

BLAST of CSPI03G37700 vs. NCBI nr
Match: KAG6580879.1 (Werner Syndrome-like exonuclease, partial [Cucurbita argyrosperma subsp. sororia] >KAG7017635.1 Werner Syndrome-like exonuclease, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 337.4 bits (864), Expect = 8.7e-89
Identity = 160/208 (76.92%), Postives = 183/208 (87.98%), Query Frame = 0


Query: 1   MAITIVDHEVPSDSHNYFDITFDTDEPILTLLTTSPSMVDGWISQTLAIQTPPLLVGLDI 60
           MA+TIVD E PS SHNYFD+TF++DEPILTLLT SP MVD WIS+TL  QTPPL+VGLDI
Sbjct: 1   MALTIVDREDPSGSHNYFDVTFESDEPILTLLTISPLMVDDWISETLNTQTPPLIVGLDI 60

Query: 61  EWRPNNRSYDNPVATLQLCIGRRCLILQLLHMPEIPKSLFEFLENESFTFVGVGIDEDAE 120
           EWRPN RSY+NPVATLQLC GRRCLI+QL++ PE PKSL+EFLENES +FVGVGI+ DAE
Sbjct: 61  EWRPNIRSYENPVATLQLCTGRRCLIVQLIYAPEFPKSLYEFLENESISFVGVGIEGDAE 120

Query: 121 KLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQE 180
           KLNCDYGLKV K +DLR+LAESVTGR ELKNAGLK L KE+LGKEI KP+ VT+SRWDQE
Sbjct: 121 KLNCDYGLKVRKTVDLRNLAESVTGRRELKNAGLKSLAKEILGKEIRKPRGVTLSRWDQE 180

Query: 181 WLTLNQVKYACIDAFFSFEIGKFLQSAS 209
           WLT NQ+KYAC DAF S+EIG+ LQ++S
Sbjct: 181 WLTHNQIKYACFDAFVSYEIGRHLQASS 208

[Close]

BLAST of CSPI03G37700 vs. TAIR 10
Match: AT3G12410.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 102.8 bits (255), Expect = 3.4e-22
Identity = 68/212 (32.08%), Postives = 112/212 (52.83%), Query Frame = 0


Query: 13  DSHNYFDITFDTDEPILTLLTTSPSMVDGWISQTL---AIQTPPLLVGLDIEW------- 72
           ++H  + + F  DE I+T +T   S++  WI   L      + PL+VG+ ++W       
Sbjct: 13  NTHLEYSVDFFGDEFIVT-VTWDSSVISRWIRNVLFNNRFSSHPLVVGVGVQWTPFSYYS 72

Query: 73  --RPNN--------RSY-DNPVATLQLCIGRRCLILQLLHMPEIPKSLFEFLENESFTFV 132
             RPNN        R Y DNP   LQLC+G RCLI+QL +  ++P +L  FL +   TFV
Sbjct: 73  DPRPNNYYADPPPIRYYSDNPADILQLCVGNRCLIIQLGYCDQVPNNLRSFLADPETTFV 132

Query: 133 GVGIDEDAEKL-NCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKE-IHKP 192
           GV   +DA KL  C + L++G+ +D+R       GR  ++ +  + + +E +G + +   
Sbjct: 133 GVWNGQDAGKLARCCHQLEIGELLDIRRYVTDSWGR-SMRRSSFEEIVEECMGYQGVMLD 192

Query: 193 KSVTMSRWDQEWLTLNQVKYACIDAFFSFEIG 202
             ++MS W    L L+Q+  A +DA+   ++G
Sbjct: 193 PEISMSDWTAYDLDLDQILQASLDAYVCHQLG 222

[Close]

BLAST of CSPI03G37700 vs. TAIR 10
Match: AT2G36110.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 99.0 bits (245), Expect = 4.9e-21
Identity = 61/182 (33.52%), Postives = 97/182 (53.30%), Query Frame = 0


Query: 26  EPILTLLTTSPSMVDGWISQ----TLAIQTPPLLVGLDIEWRPNNRSYDNPVATLQLCIG 85
           E ++  +T + S +  WI      +    + PL+VGLD++W P     D P   LQLC+G
Sbjct: 24  ERLIVTVTHTTSTIRRWIHSIRFFSRLRSSHPLVVGLDVQWTPGGS--DPPPDILQLCVG 83

Query: 86  RRCLILQLLHMPEIPKSLFEFLENESFTFVGVGIDEDAEKL-NCDYGLKVGKRMDLRDLA 145
            RCLI+QL H   IP+ L  FLE+E+ TFVGV   +D  KL    + L++ + +D+R   
Sbjct: 84  NRCLIIQLSHCKRIPEVLRSFLEDETITFVGVWNSQDQGKLERFRHQLEIWRLLDIRHYL 143

Query: 146 ESVTGRGELKNAGLKRLGKEVLG-KEIHKPKSVTMSRWDQEWLTLNQVKYACIDAFFSFE 202
            +      L N+  +++ +E LG K + K K + MS W    L+ +Q+  A  D +   +
Sbjct: 144 PT-----RLLNSSFEKIVEECLGYKGVRKDKEICMSNWGARSLSHDQIVQASDDVYVCCK 198

[Close]

BLAST of CSPI03G37700 vs. TAIR 10
Match: AT4G13870.1 (Werner syndrome-like exonuclease )

HSP 1 Score: 96.3 bits (238), Expect = 3.1e-20
Identity = 60/155 (38.71%), Postives = 89/155 (57.42%), Query Frame = 0


Query: 56  VGLDIEWRPNNRSYDNP--VATLQLCI-GRRCLILQLLHMPEIPKSLFEFLENESFTFVG 115
           VGLDIEWRP+ R    P  VAT+Q+C+    C ++ + H   IP+SL   +E+ +   VG
Sbjct: 130 VGLDIEWRPSFRKGVLPGKVATVQICVDSNYCDVMHIFH-SGIPQSLQHLIEDSTLVKVG 189

Query: 116 VGIDEDAEKLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSV 175
           +GID D+ KL  DYG+ +    DL DLA    G G+ K  GL  L + ++ KE+ KP  +
Sbjct: 190 IGIDGDSVKLFHDYGVSIKDVEDLSDLANQKIG-GD-KKWGLASLTETLVCKELLKPNRI 249

Query: 176 TMSRWDQEWLTLNQVKYACIDAFFSFEIGKFLQSA 208
            +  W+   L+  Q++YA  DA+ S+ + K L  A
Sbjct: 250 RLGNWEFYPLSKQQLQYAATDAYASWHLYKDLPDA 281

[Close]

BLAST of CSPI03G37700 vs. TAIR 10
Match: AT4G13870.2 (Werner syndrome-like exonuclease )

HSP 1 Score: 96.3 bits (238), Expect = 3.1e-20
Identity = 59/153 (38.56%), Postives = 89/153 (58.17%), Query Frame = 0


Query: 56  VGLDIEWRPNNRSYDNP--VATLQLCI-GRRCLILQLLHMPEIPKSLFEFLENESFTFVG 115
           VGLDIEWRP+ R    P  VAT+Q+C+    C ++ + H   IP+SL   +E+ +   VG
Sbjct: 130 VGLDIEWRPSFRKGVLPGKVATVQICVDSNYCDVMHIFH-SGIPQSLQHLIEDSTLVKVG 189

Query: 116 VGIDEDAEKLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSV 175
           +GID D+ KL  DYG+ +    DL DLA    G G+ K  GL  L + ++ KE+ KP  +
Sbjct: 190 IGIDGDSVKLFHDYGVSIKDVEDLSDLANQKIG-GD-KKWGLASLTETLVCKELLKPNRI 249

Query: 176 TMSRWDQEWLTLNQVKYACIDAFFSFEIGKFLQ 206
            +  W+   L+  Q++YA  DA+ S+ + K L+
Sbjct: 250 RLGNWEFYPLSKQQLQYAATDAYASWHLYKVLK 279

[Close]

BLAST of CSPI03G37700 vs. TAIR 10
Match: AT3G12470.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 95.9 bits (237), Expect = 4.1e-20
Identity = 62/193 (32.12%), Postives = 103/193 (53.37%), Query Frame = 0


Query: 14  SHNYFDITFDTDEPILTLLTTSPSMVDGWISQTLAI----QTPPLLVGLDIEWRPNNRSY 73
           +H    + F  D+ I+T +T + S++  WI    +        PL+VG+ ++WRP++   
Sbjct: 13  THQKHFVDFFGDDLIVT-VTPTASVIRRWIRSVRSYNRNHSVHPLVVGIGVQWRPDSSDP 72

Query: 74  DNPVATLQLCIGRRCLILQLLHMPEIPKSLFEFLENESFTFVGVGIDEDAEKL-NCDYGL 133
           D P  TLQLC+G RCLI+QL +   +PK L  FL +   TFVGV   +D +KL  C + +
Sbjct: 73  DPPPKTLQLCVGSRCLIIQLGYNYGLPKVLRTFLADPKTTFVGVWNGQDQKKLEKCRHRV 132

Query: 134 KVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGK-EIHKPKSVTMSRWDQEWLTLNQV 193
           ++GK +D+R       G   +     +++ KE LG+  +    ++ MS W    L   QV
Sbjct: 133 EIGKLLDIRMFVRDSRG-ARMCFCSFEQIVKERLGRVGVRLDPAICMSDWGVYNLNHYQV 192

Query: 194 KYACIDAFFSFEI 201
             A I+++  F++
Sbjct: 193 LQASIESYVCFKL 203

The following BLAST results are available for this feature:

BLAST of CSPI03G37700 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q84LH3	4.4e-19	38.56	Werner Syndrome-like exonuclease OS=Arabidopsis thaliana OX=3702 GN=WEX PE=1 SV=...	[more]
Q14191	3.0e-15	35.29	Werner syndrome ATP-dependent helicase OS=Homo sapiens OX=9606 GN=WRN PE=1 SV=2	[more]
Q9VGN7	3.3e-14	37.41	Exonuclease 3'-5' domain-containing protein 2 OS=Drosophila melanogaster OX=7227...	[more]
O09053	3.3e-14	36.30	Werner syndrome ATP-dependent helicase homolog OS=Mus musculus OX=10090 GN=Wrn P...	[more]
O93530	9.6e-14	35.48	Werner syndrome ATP-dependent helicase homolog OS=Xenopus laevis OX=8355 GN=wrn ...	[more]

BLAST of CSPI03G37700 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LBN3	1.1e-116	100.00	3'-5' exonuclease domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G...	[more]
A0A5A7UI20	2.7e-112	96.63	Werner Syndrome-like exonuclease OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
E5GBP0	2.7e-112	96.63	3'-5' exonuclease OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1	[more]
A0A1S3B738	2.7e-112	96.63	Werner Syndrome-like exonuclease OS=Cucumis melo OX=3656 GN=LOC103486879 PE=4 SV...	[more]
A0A6J1F975	3.6e-88	76.44	Werner Syndrome-like exonuclease OS=Cucurbita moschata OX=3662 GN=LOC111441964 P...	[more]

BLAST of CSPI03G37700 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011652174.1	2.2e-116	100.00	Werner Syndrome-like exonuclease [Cucumis sativus] >KGN59430.1 hypothetical prot...	[more]
XP_008443244.1	5.6e-112	96.63	PREDICTED: Werner Syndrome-like exonuclease [Cucumis melo] >ADN33882.1 3'-5' exo...	[more]
XP_038903327.1	3.1e-94	90.37	Werner Syndrome-like exonuclease isoform X2 [Benincasa hispida]	[more]
XP_038903326.1	5.3e-94	90.86	Werner Syndrome-like exonuclease isoform X1 [Benincasa hispida]	[more]
KAG6580879.1	8.7e-89	76.92	Werner Syndrome-like exonuclease, partial [Cucurbita argyrosperma subsp. sororia...	[more]

BLAST of CSPI03G37700 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G12410.1	3.4e-22	32.08	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT2G36110.1	4.9e-21	33.52	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT4G13870.1	3.1e-20	38.71	Werner syndrome-like exonuclease	[more]
AT4G13870.2	3.1e-20	38.56	Werner syndrome-like exonuclease	[more]
AT3G12470.1	4.1e-20	32.12	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002562	3'-5' exonuclease domain	SMART	SM00474	35exoneu6	coord: 36..209 e-value: 2.0E-8 score: 44.0
IPR002562	3'-5' exonuclease domain	PFAM	PF01612	DNA_pol_A_exo1	coord: 42..206 e-value: 6.5E-18 score: 65.1
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 18..206 e-value: 1.1E-56 score: 193.6
None	No IPR available	PANTHER	PTHR13620:SF65	OS01G0660800 PROTEIN	coord: 9..207
None	No IPR available	PANTHER	PTHR13620	3-5 EXONUCLEASE	coord: 9..207
None	No IPR available	CDD	cd06141	WRN_exo	coord: 33..202 e-value: 9.29726E-64 score: 193.179
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 51..205

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G37700.1	CSPI03G37700.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0090305	nucleic acid phosphodiester bond hydrolysis
biological_process	GO:0006139	nucleobase-containing compound metabolic process
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005634	nucleus
molecular_function	GO:0008408	3'-5' exonuclease activity
molecular_function	GO:0003676	nucleic acid binding