CSPI03G36120 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI03G36120
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Mediator of RNA polymerase II transcription subunit 7
Location	Chr3: 31462749 .. 31466067 (+)
RNA-Seq Expression	CSPI03G36120
Synteny	CSPI03G36120

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTTTTATAATTTTCCTTCGGAACGCATCGGTTCTTGTTAGATTTTTTTCCGATCAAGACTAAACCATGGCGACAGCTACCTATCCACCACCACCTCCATTTTACAAGCTCTACAAAGATTACCTGCAGGATCCGAAATCGGCACCGGAGCCTCCTCCGCCCATCGAAGGCACCTATATGTGCTTTGGTAGCAATTACACCGTGAGATTTACTCTACATTCCTTTTGAGTATCGTGATTCCGTTTTCATTTCACACATTAGTTTAGTTCTTTTACCAGTTTGCGGTTTCGTTGTACGTTTCATCTTTAATTCCATAGAGTTGAAGGGATGTATGCATGTTCAAAATGTATTTAGGGTTTTCAGCTCTGAAGTTGTTGGATTCCGCTTCCAAAATCCTTCATATTTTTCTCATTTTAGTGCGGTTTCCTTGTATTTGAATCTCGAGAGTCGGTTTTGATTTCACACCCTAGTCGAATTCTTTTAGCAGTTTGAGTTTAACTGTATTTTTCAACTTTAATTCCATAGATTTGAAGGGGGACGAATGTATGTTCAGAATGTATTTAGGGTTTTTCAGTTCTGAAGTTGTTGAATTCTGCTTTAAAAATGCTTCATATTATTCTCATTTTAATGCGGATTTCTTGCATGTTTTGATTGAGTTGATAATTTAAACATTGTCACGATAATTTGAATATCGAGATTCGGTTTTGATTTCAGACATTAGTTGAGTTCTTTTAGCAGTTTCAGTTTAATTGTATTTTTCATCTTTAATTCCATAGAGTTGGAGGGGACGTATGTATCTGCAAAGTGTATTTGGGGTTTTTCACTTCTGAAGTTGTTGGGTTACGCTTCCAAAATCCTTCATATTATTATCATTTTACTGCGGAATCCTTGTATATTTTGATTGGATTGATTATTGAAACAGCTTCACGATAATTGCTCTGTTTTTTTATTCTTTATCTTGATTATTCTGTTGTGATTTTAGACGGATGACGTGCTTCCAAGTTTGGAAGAGCAAGGAGTGCGCCAACTTTACCCTAGAGGGCCAAATGTTGGTAAGTATGTTTAGTTATCAGTGCTTCCAAATTGTTCTCCATCAGTGGTTTTCTATTCAATAAGAACTGTTATTTGTCTCTCAATTAAGTTAGTGTTATGCCCTAGCATTTATCTTCTGATTCCTAAGGTTCGTGGTCTCTGTTTTTGTTGGTTGATAAGAAAAGTGGATAGACTCATAGTCCCCAAGGGATGGTCACATTGTTTAAAAGGCCTGTTGTCTTTGGGAAAAACCTACTCATAGTTTTTGTTTTTGAAGCTGGAAGCCATGGGTTTTTAAAAGAATAGAATACTTGTATTGGAGGAGGAGGGATGCTGAAGTGAAGGAACAATTTGCAATCCATCCCCATGCCAAGATTTAAAGGTTGAGGCAAATTGTAAAGAAAATGTGCAATTAGCACATGCAAAAATCAAAGTACAGCTAAAAAAAAAAGATTTGATGAAGATTACCAGCTAGATTAGTAGACTTAGCCCTATCTAAATTAAATTACAGAGACACACACGAGTCCCAAGACTCCTACATCATACATGTGCACGCTTACACCCACTCATACACATCCTTTTATCTACATTTTTAATCACGAAAGCTTTGCTTTACAATTGAACCTAGAACTGCAGGCAGGGAATAATTAATCAGTTGGGTTTTTTTTTTAATAAAAAAAAGGTCCGATAATTTCCCCCAGTCTCTGAGCACCAGTGTGATTGTTTTAAGGCCTTGACAATCGGGTTGGGATTTTCAGATTTCTATTTCTGTGTGAGTATTTGAGTGGTTCTATTCGTGATTGTGAAGACATGTGGTACTGTATTTATAGACATGAAATGTGTTAGTGGTATTGGTTGTAATATTCTTTATAGATCTAAAATAAAGACCCTCTTTACCTACGACACTTGCTCAATGAACTTGAATGGAGTGCTTGGAGTAACTCTCTACAGGCCTGCTCCTTAAACTAACTCACTTAGCTGACATTATTACCTGTGGTTCTTTTTCAGATTATAAGAAGGAATTGAGATCTCTGAATAGAGAATTGCAGCTTCACATTCTGGAGCTTGCTGATATTCTTGTTGAGAGGCCATCCCAATATGCAAGGAGGGTGGAAGAAATATCCCTAATATTCAAAAACTTACACCATCTGCTTAATTCTTTGCGACCACATCAGGTGGACACTTTAAAACATTTTAAATTAGTGTATCCATATTGGTGAAATCATTTCATGTTTATTGTTTATTTTTCCCTGTAGGCTAGAGCAACATTGATTCATATACTAGAACTTCAGATTGAGCGCCGTAGACAAGCTGTCGAGGATATAAAGAGGTATGCTAATAAGGGTTCTGATGGAATATATCAGAAATAAGAATGTGGAAGAATTGATACAATCACTAACCCTTTCACCACACCTCTTTTGATACATTATAAAATATCTCGGGCAAACTTAATTGCACATACAAGTTGGGACTTTTGGCCCCTTTATCAATAATCATTGTTTCTTATCATATTCTTGGTGGTATGAGTCTAGTTGGTGGAGATATGTAGCATTGTAAAATTTTCAGCTAACGATTCAATGTTCTAATTACAAAGTCTTGATTTCCATTTCGAGGCACCTCTTCATTGATTATTTTGCTACTTTTTGAATCACGGTTTGGCTTCTTCAATTATGTACTTTGCCTAGGAGGAGAGAAGAAGCACAAAGACTGCTCATGGAGTCACTTGAAACTTTGGATGGGCACTAGTACTACCTCTTTCATCAGTGTTGAATTCATAAAGTACAGATCTTGTCCATGCTGCATGTTTCGATCTCCTCGTATTCTAGTGCATCTACTGATTTCATCGGTTCAGCTCAGTCAGTTCTAATGCATACACTGGTGCATCGATGATATGTCTAGGTAATTCTTAGGTATAACATCTTTGTCAATCTGTGCAAGATCCAGAAGCGACCTCTCCCCCATTGTGATTACTTGAGCAGATTGTTAATCAGATTGTAATTAATTGTTTGAACTACATTGTAAGCTTTAGTTCACGGGTTCGGCATAGTGACTAAGAAACGCAGTTGAAAAGATCTGTTCTATAGATTGTACTGAGTGACAGCCTATAACCGTGAGATTGGAAGAAATTAATGCTATTGCGTCCTTGGCTTCCGTAATGTTTCTCTCACTATGCACTATTGTTTGAAATACAACGATCCGCCAGGCTGTGGGATGCAGCAGTAACGATGGAGACAATGGTAACTTATCAATGAATAATTTGTTTGGTCAATTATTGATACTTTTTTATA

mRNA sequence

CTTTTATAATTTTCCTTCGGAACGCATCGGTTCTTGTTAGATTTTTTTCCGATCAAGACTAAACCATGGCGACAGCTACCTATCCACCACCACCTCCATTTTACAAGCTCTACAAAGATTACCTGCAGGATCCGAAATCGGCACCGGAGCCTCCTCCGCCCATCGAAGGCACCTATATGTGCTTTGGTAGCAATTACACCACGGATGACGTGCTTCCAAGTTTGGAAGAGCAAGGAGTGCGCCAACTTTACCCTAGAGGGCCAAATGTTGATTATAAGAAGGAATTGAGATCTCTGAATAGAGAATTGCAGCTTCACATTCTGGAGCTTGCTGATATTCTTGTTGAGAGGCCATCCCAATATGCAAGGAGGGTGGAAGAAATATCCCTAATATTCAAAAACTTACACCATCTGCTTAATTCTTTGCGACCACATCAGGCTAGAGCAACATTGATTCATATACTAGAACTTCAGATTGAGCGCCGTAGACAAGCTGTCGAGGATATAAAGAGGAGGAGAGAAGAAGCACAAAGACTGCTCATGGAGTCACTTGAAACTTTGGATGGGCACTAGTACTACCTCTTTCATCAGTGTTGAATTCATAAAGTACAGATCTTGTCCATGCTGCATGTTTCGATCTCCTCGTATTCTAGTGCATCTACTGATTTCATCGGTTCAGCTCAGTCAGTTCTAATGCATACACTGGTGCATCGATGATATGTCTAGGTAATTCTTAGGTATAACATCTTTGTCAATCTGTGCAAGATCCAGAAGCGACCTCTCCCCCATTGTGATTACTTGAGCAGATTGTTAATCAGATTGTAATTAATTGTTTGAACTACATTGTAAGCTTTAGTTCACGGGTTCGGCATAGTGACTAAGAAACGCAGTTGAAAAGATCTGTTCTATAGATTGTACTGAGTGACAGCCTATAACCGTGAGATTGGAAGAAATTAATGCTATTGCGTCCTTGGCTTCCGTAATGTTTCTCTCACTATGCACTATTGTTTGAAATACAACGATCCGCCAGGCTGTGGGATGCAGCAGTAACGATGGAGACAATGGTAACTTATCAATGAATAATTTGTTTGGTCAATTATTGATACTTTTTTATA

Coding sequence (CDS)

ATGGCGACAGCTACCTATCCACCACCACCTCCATTTTACAAGCTCTACAAAGATTACCTGCAGGATCCGAAATCGGCACCGGAGCCTCCTCCGCCCATCGAAGGCACCTATATGTGCTTTGGTAGCAATTACACCACGGATGACGTGCTTCCAAGTTTGGAAGAGCAAGGAGTGCGCCAACTTTACCCTAGAGGGCCAAATGTTGATTATAAGAAGGAATTGAGATCTCTGAATAGAGAATTGCAGCTTCACATTCTGGAGCTTGCTGATATTCTTGTTGAGAGGCCATCCCAATATGCAAGGAGGGTGGAAGAAATATCCCTAATATTCAAAAACTTACACCATCTGCTTAATTCTTTGCGACCACATCAGGCTAGAGCAACATTGATTCATATACTAGAACTTCAGATTGAGCGCCGTAGACAAGCTGTCGAGGATATAAAGAGGAGGAGAGAAGAAGCACAAAGACTGCTCATGGAGTCACTTGAAACTTTGGATGGGCACTAG

Protein sequence

MATATYPPPPPFYKLYKDYLQDPKSAPEPPPPIEGTYMCFGSNYTTDDVLPSLEEQGVRQLYPRGPNVDYKKELRSLNRELQLHILELADILVERPSQYARRVEEISLIFKNLHHLLNSLRPHQARATLIHILELQIERRRQAVEDIKRRREEAQRLLMESLETLDGH*

Homology

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BLAST of CSPI03G36120 vs. ExPASy Swiss-Prot
Match: Q9LYW3 (Mediator of RNA polymerase II transcription subunit 7a OS=Arabidopsis thaliana OX=3702 GN=MED7A PE=1 SV=1)

HSP 1 Score: 282.7 bits (722), Expect = 2.7e-75
Identity = 137/167 (82.04%), Postives = 155/167 (92.81%), Query Frame = 0


Query: 1   MATATYPPPPPFYKLYKDYLQDPKSAPEPPPPIEGTYMCFGSNYTTDDVLPSLEEQGVRQ 60
           MATATYPPPPP+Y+LYKDY ++P SAPEPPPPIEGTY+CFG NYTT+DVLPSLEEQGV Q
Sbjct: 1   MATATYPPPPPYYRLYKDYSENPNSAPEPPPPIEGTYVCFGGNYTTEDVLPSLEEQGVPQ 60

Query: 61  LYPRGPNVDYKKELRSLNRELQLHILELADILVERPSQYARRVEEISLIFKNLHHLLNSL 120
           LYP+  N+DYK ELRSLNRELQLHILELAD+LV+RPSQYA+R+ EIS IFKNLHHLLNSL
Sbjct: 61  LYPKDSNLDYKNELRSLNRELQLHILELADVLVDRPSQYAKRIGEISSIFKNLHHLLNSL 120

Query: 121 RPHQARATLIHILELQIERRRQAVEDIKRRREEAQRLLMESLETLDG 168
           RPHQARATLIHI+ELQI++R+QAVEDIKRRREEAQRLL ++  TLDG
Sbjct: 121 RPHQARATLIHIMELQIQQRKQAVEDIKRRREEAQRLLKDAYLTLDG 167

[Close]

BLAST of CSPI03G36120 vs. ExPASy Swiss-Prot
Match: Q9LZD7 (Mediator of RNA polymerase II transcription subunit 7b OS=Arabidopsis thaliana OX=3702 GN=MED7B PE=1 SV=1)

HSP 1 Score: 277.7 bits (709), Expect = 8.6e-74
Identity = 135/167 (80.84%), Postives = 154/167 (92.22%), Query Frame = 0


Query: 1   MATATYPPPPPFYKLYKDYLQDPKSAPEPPPPIEGTYMCFGSNYTTDDVLPSLEEQGVRQ 60
           MATATYPPPPP+Y+LYKD+ ++  SAPEPPPPIEGTY+CFG NYTT+DVLPSLEEQGV Q
Sbjct: 1   MATATYPPPPPYYRLYKDFSENTDSAPEPPPPIEGTYVCFGGNYTTEDVLPSLEEQGVPQ 60

Query: 61  LYPRGPNVDYKKELRSLNRELQLHILELADILVERPSQYARRVEEISLIFKNLHHLLNSL 120
           LYP+  N+DYKKELRSLNRELQLHILELAD+LV+RPSQYA+R+ EIS IFKNLHHLLNSL
Sbjct: 61  LYPKDSNLDYKKELRSLNRELQLHILELADVLVDRPSQYAKRIGEISSIFKNLHHLLNSL 120

Query: 121 RPHQARATLIHILELQIERRRQAVEDIKRRREEAQRLLMESLETLDG 168
           RPHQARATLIHI+ELQI++R+QAVEDIKRRREEAQ LL ++  TLDG
Sbjct: 121 RPHQARATLIHIMELQIQQRKQAVEDIKRRREEAQGLLKDAFVTLDG 167

[Close]

BLAST of CSPI03G36120 vs. ExPASy Swiss-Prot
Match: Q55DQ5 (Putative mediator of RNA polymerase II transcription subunit 7 OS=Dictyostelium discoideum OX=44689 GN=med7 PE=3 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 5.5e-28
Identity = 63/141 (44.68%), Postives = 94/141 (66.67%), Query Frame = 0


Query: 26  APEPPPPIEGTYMCFGSNYTTDDVLPSLEEQGVRQLYPRGPNVDYKKELRSLNRELQLHI 85
           AP  PP +   Y+ FG  Y+T D+LPSL+E G +QLYP+G +++   EL+ LNR +  + 
Sbjct: 143 APPLPPKVGANYVQFGQTYSTVDMLPSLDESGAKQLYPKG-DIEPISELKKLNRSILFNY 202

Query: 86  LELADILVERPSQYARRVEEISLIFKNLHHLLNSLRPHQARATLIHILELQIERRRQAVE 145
           L+L + L+E P  Y  ++E+ISL+F N HHLLNS RPHQAR TL+ I+  QI+++ Q+ E
Sbjct: 203 LQLLETLIENPKNYQSKIEDISLLFINFHHLLNSYRPHQARETLLSIMNEQIKQKNQSNE 262

Query: 146 DIKRRREEAQRLLMESLETLD 167
            IK+  +  +  LM S + L+
Sbjct: 263 SIKKALDICKESLMNSFKNLN 282

[Close]

BLAST of CSPI03G36120 vs. ExPASy Swiss-Prot
Match: Q17MI0 (Mediator of RNA polymerase II transcription subunit 7 OS=Aedes aegypti OX=7159 GN=MED7 PE=3 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 1.4e-26
Identity = 64/161 (39.75%), Postives = 103/161 (63.98%), Query Frame = 0


Query: 8   PPPPFYKLYKDYLQDPKSAPEPPPPIEGTYMCFGSNYTTDD-VLPSLEEQGVRQLYPRGP 67
           PP  +YKLY D       AP+PP PI+GTY  FG  ++T+D ++  LE QG ++LYP+  
Sbjct: 15  PPAQYYKLYTDENVRNNRAPKPPAPIQGTYQMFGQQFSTEDNIIRPLEAQGFKRLYPQ-- 74

Query: 68  NVDYKKELRSLNRELQLHILELADILVERPS--QYARRVEEISLIFKNLHHLLNSLRPHQ 127
           + D +KEL+ LN  L ++ L+L D+LV  P   Q A ++E+++L+F ++HHLLN  RPHQ
Sbjct: 75  HFDRRKELKKLNHSLLVNFLDLIDLLVHNPDSPQRAEKIEDLNLLFVHIHHLLNEFRPHQ 134

Query: 128 ARATLIHILELQIERRRQAVEDIKRRREEAQRLLMESLETL 166
           AR TL  ++ELQ  +R++  +  +   E+ + ++  +  +L
Sbjct: 135 ARETLRVMMELQKRQRQETTQRFQNHLEKVREMVNNAFASL 173

[Close]

BLAST of CSPI03G36120 vs. ExPASy Swiss-Prot
Match: Q3T123 (Mediator of RNA polymerase II transcription subunit 7 OS=Bos taurus OX=9913 GN=MED7 PE=2 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 3.7e-24
Identity = 63/166 (37.95%), Postives = 99/166 (59.64%), Query Frame = 0


Query: 3   TATYPPPPPFYKLYKDYLQDPKSAPEPPPPIEGTYMCFGSNYTTDD-VLPSLEEQGVRQL 62
           +A  PPP  + K Y D       AP+PPPPI+ +YM FG+ +  DD ++  LE QG+ +L
Sbjct: 8   SALPPPPMQYIKEYTDENIQEGLAPKPPPPIKDSYMMFGNQFQCDDLIIRPLESQGIERL 67

Query: 63  YPRGPNVDYKKELRSLNRELQLHILELADILVERPSQYAR--RVEEISLIFKNLHHLLNS 122
           +P     D+KKELR LN  + ++ L+L DIL+  P    R  ++E++ L+F ++HHL+N 
Sbjct: 68  HPM--QFDHKKELRKLNMSILINFLDLLDILIRSPGSIKREEKLEDLKLLFVHVHHLINE 127

Query: 123 LRPHQARATLIHILELQIERRRQAVEDIKRRREEAQRLLMESLETL 166
            RPHQAR TL  ++E+Q  +R +  E  ++  E    ++   L +L
Sbjct: 128 YRPHQARETLRVMMEVQKRQRLETAERFQKHLERVIEMIQNCLASL 171

[Close]

BLAST of CSPI03G36120 vs. ExPASy TrEMBL
Match: A0A5A7TKB8 (Mediator of RNA polymerase II transcription subunit 7 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G005090 PE=3 SV=1)

HSP 1 Score: 335.1 bits (858), Expect = 1.7e-88
Identity = 168/168 (100.00%), Postives = 168/168 (100.00%), Query Frame = 0


Query: 1   MATATYPPPPPFYKLYKDYLQDPKSAPEPPPPIEGTYMCFGSNYTTDDVLPSLEEQGVRQ 60
           MATATYPPPPPFYKLYKDYLQDPKSAPEPPPPIEGTYMCFGSNYTTDDVLPSLEEQGVRQ
Sbjct: 1   MATATYPPPPPFYKLYKDYLQDPKSAPEPPPPIEGTYMCFGSNYTTDDVLPSLEEQGVRQ 60

Query: 61  LYPRGPNVDYKKELRSLNRELQLHILELADILVERPSQYARRVEEISLIFKNLHHLLNSL 120
           LYPRGPNVDYKKELRSLNRELQLHILELADILVERPSQYARRVEEISLIFKNLHHLLNSL
Sbjct: 61  LYPRGPNVDYKKELRSLNRELQLHILELADILVERPSQYARRVEEISLIFKNLHHLLNSL 120

Query: 121 RPHQARATLIHILELQIERRRQAVEDIKRRREEAQRLLMESLETLDGH 169
           RPHQARATLIHILELQIERRRQAVEDIKRRREEAQRLLMESLETLDGH
Sbjct: 121 RPHQARATLIHILELQIERRRQAVEDIKRRREEAQRLLMESLETLDGH 168

[Close]

BLAST of CSPI03G36120 vs. ExPASy TrEMBL
Match: A0A0A0LBQ7 (Mediator of RNA polymerase II transcription subunit 7 OS=Cucumis sativus OX=3659 GN=Csa_3G791540 PE=3 SV=1)

HSP 1 Score: 335.1 bits (858), Expect = 1.7e-88
Identity = 168/168 (100.00%), Postives = 168/168 (100.00%), Query Frame = 0


Query: 1   MATATYPPPPPFYKLYKDYLQDPKSAPEPPPPIEGTYMCFGSNYTTDDVLPSLEEQGVRQ 60
           MATATYPPPPPFYKLYKDYLQDPKSAPEPPPPIEGTYMCFGSNYTTDDVLPSLEEQGVRQ
Sbjct: 1   MATATYPPPPPFYKLYKDYLQDPKSAPEPPPPIEGTYMCFGSNYTTDDVLPSLEEQGVRQ 60

Query: 61  LYPRGPNVDYKKELRSLNRELQLHILELADILVERPSQYARRVEEISLIFKNLHHLLNSL 120
           LYPRGPNVDYKKELRSLNRELQLHILELADILVERPSQYARRVEEISLIFKNLHHLLNSL
Sbjct: 61  LYPRGPNVDYKKELRSLNRELQLHILELADILVERPSQYARRVEEISLIFKNLHHLLNSL 120

Query: 121 RPHQARATLIHILELQIERRRQAVEDIKRRREEAQRLLMESLETLDGH 169
           RPHQARATLIHILELQIERRRQAVEDIKRRREEAQRLLMESLETLDGH
Sbjct: 121 RPHQARATLIHILELQIERRRQAVEDIKRRREEAQRLLMESLETLDGH 168

[Close]

BLAST of CSPI03G36120 vs. ExPASy TrEMBL
Match: A0A1S3B7S4 (Mediator of RNA polymerase II transcription subunit 7 OS=Cucumis melo OX=3656 GN=LOC103486732 PE=3 SV=1)

HSP 1 Score: 335.1 bits (858), Expect = 1.7e-88
Identity = 168/168 (100.00%), Postives = 168/168 (100.00%), Query Frame = 0


Query: 1   MATATYPPPPPFYKLYKDYLQDPKSAPEPPPPIEGTYMCFGSNYTTDDVLPSLEEQGVRQ 60
           MATATYPPPPPFYKLYKDYLQDPKSAPEPPPPIEGTYMCFGSNYTTDDVLPSLEEQGVRQ
Sbjct: 1   MATATYPPPPPFYKLYKDYLQDPKSAPEPPPPIEGTYMCFGSNYTTDDVLPSLEEQGVRQ 60

Query: 61  LYPRGPNVDYKKELRSLNRELQLHILELADILVERPSQYARRVEEISLIFKNLHHLLNSL 120
           LYPRGPNVDYKKELRSLNRELQLHILELADILVERPSQYARRVEEISLIFKNLHHLLNSL
Sbjct: 61  LYPRGPNVDYKKELRSLNRELQLHILELADILVERPSQYARRVEEISLIFKNLHHLLNSL 120

Query: 121 RPHQARATLIHILELQIERRRQAVEDIKRRREEAQRLLMESLETLDGH 169
           RPHQARATLIHILELQIERRRQAVEDIKRRREEAQRLLMESLETLDGH
Sbjct: 121 RPHQARATLIHILELQIERRRQAVEDIKRRREEAQRLLMESLETLDGH 168

[Close]

BLAST of CSPI03G36120 vs. ExPASy TrEMBL
Match: A0A6J1DBY3 (Mediator of RNA polymerase II transcription subunit 7 OS=Momordica charantia OX=3673 GN=LOC111019312 PE=3 SV=1)

HSP 1 Score: 325.5 bits (833), Expect = 1.3e-85
Identity = 162/168 (96.43%), Postives = 166/168 (98.81%), Query Frame = 0


Query: 1   MATATYPPPPPFYKLYKDYLQDPKSAPEPPPPIEGTYMCFGSNYTTDDVLPSLEEQGVRQ 60
           MATATYPPPPPFY+LYKDYLQ+PKSAPEPPPPIEGTY CFGSNYTTDDVLPSLEEQGVRQ
Sbjct: 1   MATATYPPPPPFYRLYKDYLQNPKSAPEPPPPIEGTYACFGSNYTTDDVLPSLEEQGVRQ 60

Query: 61  LYPRGPNVDYKKELRSLNRELQLHILELADILVERPSQYARRVEEISLIFKNLHHLLNSL 120
           LYPRGPNVD+KKELRSLNRELQLHILELADILVERPSQYARRVEEISLIFKNLHHLLNSL
Sbjct: 61  LYPRGPNVDFKKELRSLNRELQLHILELADILVERPSQYARRVEEISLIFKNLHHLLNSL 120

Query: 121 RPHQARATLIHILELQIERRRQAVEDIKRRREEAQRLLMESLETLDGH 169
           RPHQARATLIHILELQI+RRRQAVEDIKRRREEAQRL MESLETLDGH
Sbjct: 121 RPHQARATLIHILELQIDRRRQAVEDIKRRREEAQRLFMESLETLDGH 168

[Close]

BLAST of CSPI03G36120 vs. ExPASy TrEMBL
Match: A0A6J1FG49 (Mediator of RNA polymerase II transcription subunit 7 OS=Cucurbita moschata OX=3662 GN=LOC111443847 PE=3 SV=1)

HSP 1 Score: 325.5 bits (833), Expect = 1.3e-85
Identity = 162/168 (96.43%), Postives = 167/168 (99.40%), Query Frame = 0


Query: 1   MATATYPPPPPFYKLYKDYLQDPKSAPEPPPPIEGTYMCFGSNYTTDDVLPSLEEQGVRQ 60
           MATATYPPPPP+Y+LYKDY Q+PKSAPEPPPPIEGTY+CFGSNYTTDDVLPSLEEQGVRQ
Sbjct: 1   MATATYPPPPPYYRLYKDYSQNPKSAPEPPPPIEGTYVCFGSNYTTDDVLPSLEEQGVRQ 60

Query: 61  LYPRGPNVDYKKELRSLNRELQLHILELADILVERPSQYARRVEEISLIFKNLHHLLNSL 120
           LYPRGPNVD+KKELRSLNRELQLHILELADILVERPSQYARRVEEISLIFKNLHHLLNSL
Sbjct: 61  LYPRGPNVDFKKELRSLNRELQLHILELADILVERPSQYARRVEEISLIFKNLHHLLNSL 120

Query: 121 RPHQARATLIHILELQIERRRQAVEDIKRRREEAQRLLMESLETLDGH 169
           RPHQARATLIHILELQIERRRQAVEDIKRRREEAQRLLMESLETLDGH
Sbjct: 121 RPHQARATLIHILELQIERRRQAVEDIKRRREEAQRLLMESLETLDGH 168

[Close]

BLAST of CSPI03G36120 vs. NCBI nr
Match: XP_008443001.1 (PREDICTED: mediator of RNA polymerase II transcription subunit 7a-like [Cucumis melo] >KAA0043764.1 mediator of RNA polymerase II transcription subunit 7a [Cucumis melo var. makuwa] >TYK25369.1 mediator of RNA polymerase II transcription subunit 7a [Cucumis melo var. makuwa])

HSP 1 Score: 335.1 bits (858), Expect = 3.5e-88
Identity = 168/168 (100.00%), Postives = 168/168 (100.00%), Query Frame = 0


Query: 1   MATATYPPPPPFYKLYKDYLQDPKSAPEPPPPIEGTYMCFGSNYTTDDVLPSLEEQGVRQ 60
           MATATYPPPPPFYKLYKDYLQDPKSAPEPPPPIEGTYMCFGSNYTTDDVLPSLEEQGVRQ
Sbjct: 1   MATATYPPPPPFYKLYKDYLQDPKSAPEPPPPIEGTYMCFGSNYTTDDVLPSLEEQGVRQ 60

Query: 61  LYPRGPNVDYKKELRSLNRELQLHILELADILVERPSQYARRVEEISLIFKNLHHLLNSL 120
           LYPRGPNVDYKKELRSLNRELQLHILELADILVERPSQYARRVEEISLIFKNLHHLLNSL
Sbjct: 61  LYPRGPNVDYKKELRSLNRELQLHILELADILVERPSQYARRVEEISLIFKNLHHLLNSL 120

Query: 121 RPHQARATLIHILELQIERRRQAVEDIKRRREEAQRLLMESLETLDGH 169
           RPHQARATLIHILELQIERRRQAVEDIKRRREEAQRLLMESLETLDGH
Sbjct: 121 RPHQARATLIHILELQIERRRQAVEDIKRRREEAQRLLMESLETLDGH 168

[Close]

BLAST of CSPI03G36120 vs. NCBI nr
Match: XP_031737683.1 (LOW QUALITY PROTEIN: mediator of RNA polymerase II transcription subunit 7a-like [Cucumis sativus])

HSP 1 Score: 332.0 bits (850), Expect = 2.9e-87
Identity = 166/168 (98.81%), Postives = 168/168 (100.00%), Query Frame = 0


Query: 1   MATATYPPPPPFYKLYKDYLQDPKSAPEPPPPIEGTYMCFGSNYTTDDVLPSLEEQGVRQ 60
           MATATYPPPPPFYKLYKDYLQDPKSAPEPPPPIEGTYMCFGSNYTTDDVLPSLEEQGVRQ
Sbjct: 1   MATATYPPPPPFYKLYKDYLQDPKSAPEPPPPIEGTYMCFGSNYTTDDVLPSLEEQGVRQ 60

Query: 61  LYPRGPNVDYKKELRSLNRELQLHILELADILVERPSQYARRVEEISLIFKNLHHLLNSL 120
           LYPRGPNVDY++ELRSLNRELQLHILELADILVERPSQYARRVEEISLIFKNLHHLLNSL
Sbjct: 61  LYPRGPNVDYQEELRSLNRELQLHILELADILVERPSQYARRVEEISLIFKNLHHLLNSL 120

Query: 121 RPHQARATLIHILELQIERRRQAVEDIKRRREEAQRLLMESLETLDGH 169
           RPHQARATLIHILELQIERRRQAVEDIKRRREEAQRLLMESLETLDGH
Sbjct: 121 RPHQARATLIHILELQIERRRQAVEDIKRRREEAQRLLMESLETLDGH 168

[Close]

BLAST of CSPI03G36120 vs. NCBI nr
Match: XP_004136548.2 (mediator of RNA polymerase II transcription subunit 7a [Cucumis sativus] >KAE8645773.1 hypothetical protein Csa_017289 [Cucumis sativus])

HSP 1 Score: 328.6 bits (841), Expect = 3.3e-86
Identity = 167/168 (99.40%), Postives = 167/168 (99.40%), Query Frame = 0


Query: 1   MATATYPPPPPFYKLYKDYLQDPKSAPEPPPPIEGTYMCFGSNYTTDDVLPSLEEQGVRQ 60
           MATATY PPPPFYKLYKDYLQDPKSAPEPPPPIEGTYMCFGSNYTTDDVLPSLEEQGVRQ
Sbjct: 1   MATATY-PPPPFYKLYKDYLQDPKSAPEPPPPIEGTYMCFGSNYTTDDVLPSLEEQGVRQ 60

Query: 61  LYPRGPNVDYKKELRSLNRELQLHILELADILVERPSQYARRVEEISLIFKNLHHLLNSL 120
           LYPRGPNVDYKKELRSLNRELQLHILELADILVERPSQYARRVEEISLIFKNLHHLLNSL
Sbjct: 61  LYPRGPNVDYKKELRSLNRELQLHILELADILVERPSQYARRVEEISLIFKNLHHLLNSL 120

Query: 121 RPHQARATLIHILELQIERRRQAVEDIKRRREEAQRLLMESLETLDGH 169
           RPHQARATLIHILELQIERRRQAVEDIKRRREEAQRLLMESLETLDGH
Sbjct: 121 RPHQARATLIHILELQIERRRQAVEDIKRRREEAQRLLMESLETLDGH 167

[Close]

BLAST of CSPI03G36120 vs. NCBI nr
Match: XP_022937468.1 (mediator of RNA polymerase II transcription subunit 7a [Cucurbita moschata] >KAG6596657.1 Mediator of RNA polymerase II transcription subunit 7b, partial [Cucurbita argyrosperma subsp. sororia] >KAG7028195.1 Mediator of RNA polymerase II transcription subunit 7b [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 325.5 bits (833), Expect = 2.8e-85
Identity = 162/168 (96.43%), Postives = 167/168 (99.40%), Query Frame = 0


Query: 1   MATATYPPPPPFYKLYKDYLQDPKSAPEPPPPIEGTYMCFGSNYTTDDVLPSLEEQGVRQ 60
           MATATYPPPPP+Y+LYKDY Q+PKSAPEPPPPIEGTY+CFGSNYTTDDVLPSLEEQGVRQ
Sbjct: 1   MATATYPPPPPYYRLYKDYSQNPKSAPEPPPPIEGTYVCFGSNYTTDDVLPSLEEQGVRQ 60

Query: 61  LYPRGPNVDYKKELRSLNRELQLHILELADILVERPSQYARRVEEISLIFKNLHHLLNSL 120
           LYPRGPNVD+KKELRSLNRELQLHILELADILVERPSQYARRVEEISLIFKNLHHLLNSL
Sbjct: 61  LYPRGPNVDFKKELRSLNRELQLHILELADILVERPSQYARRVEEISLIFKNLHHLLNSL 120

Query: 121 RPHQARATLIHILELQIERRRQAVEDIKRRREEAQRLLMESLETLDGH 169
           RPHQARATLIHILELQIERRRQAVEDIKRRREEAQRLLMESLETLDGH
Sbjct: 121 RPHQARATLIHILELQIERRRQAVEDIKRRREEAQRLLMESLETLDGH 168

[Close]

BLAST of CSPI03G36120 vs. NCBI nr
Match: XP_022151348.1 (mediator of RNA polymerase II transcription subunit 7a-like [Momordica charantia])

HSP 1 Score: 325.5 bits (833), Expect = 2.8e-85
Identity = 162/168 (96.43%), Postives = 166/168 (98.81%), Query Frame = 0


Query: 1   MATATYPPPPPFYKLYKDYLQDPKSAPEPPPPIEGTYMCFGSNYTTDDVLPSLEEQGVRQ 60
           MATATYPPPPPFY+LYKDYLQ+PKSAPEPPPPIEGTY CFGSNYTTDDVLPSLEEQGVRQ
Sbjct: 1   MATATYPPPPPFYRLYKDYLQNPKSAPEPPPPIEGTYACFGSNYTTDDVLPSLEEQGVRQ 60

Query: 61  LYPRGPNVDYKKELRSLNRELQLHILELADILVERPSQYARRVEEISLIFKNLHHLLNSL 120
           LYPRGPNVD+KKELRSLNRELQLHILELADILVERPSQYARRVEEISLIFKNLHHLLNSL
Sbjct: 61  LYPRGPNVDFKKELRSLNRELQLHILELADILVERPSQYARRVEEISLIFKNLHHLLNSL 120

Query: 121 RPHQARATLIHILELQIERRRQAVEDIKRRREEAQRLLMESLETLDGH 169
           RPHQARATLIHILELQI+RRRQAVEDIKRRREEAQRL MESLETLDGH
Sbjct: 121 RPHQARATLIHILELQIDRRRQAVEDIKRRREEAQRLFMESLETLDGH 168

[Close]

BLAST of CSPI03G36120 vs. TAIR 10
Match: AT5G03220.1 (Mediator complex, subunit Med7 )

HSP 1 Score: 282.7 bits (722), Expect = 1.9e-76
Identity = 137/167 (82.04%), Postives = 155/167 (92.81%), Query Frame = 0


Query: 1   MATATYPPPPPFYKLYKDYLQDPKSAPEPPPPIEGTYMCFGSNYTTDDVLPSLEEQGVRQ 60
           MATATYPPPPP+Y+LYKDY ++P SAPEPPPPIEGTY+CFG NYTT+DVLPSLEEQGV Q
Sbjct: 1   MATATYPPPPPYYRLYKDYSENPNSAPEPPPPIEGTYVCFGGNYTTEDVLPSLEEQGVPQ 60

Query: 61  LYPRGPNVDYKKELRSLNRELQLHILELADILVERPSQYARRVEEISLIFKNLHHLLNSL 120
           LYP+  N+DYK ELRSLNRELQLHILELAD+LV+RPSQYA+R+ EIS IFKNLHHLLNSL
Sbjct: 61  LYPKDSNLDYKNELRSLNRELQLHILELADVLVDRPSQYAKRIGEISSIFKNLHHLLNSL 120

Query: 121 RPHQARATLIHILELQIERRRQAVEDIKRRREEAQRLLMESLETLDG 168
           RPHQARATLIHI+ELQI++R+QAVEDIKRRREEAQRLL ++  TLDG
Sbjct: 121 RPHQARATLIHIMELQIQQRKQAVEDIKRRREEAQRLLKDAYLTLDG 167

[Close]

BLAST of CSPI03G36120 vs. TAIR 10
Match: AT5G03500.1 (Mediator complex, subunit Med7 )

HSP 1 Score: 277.7 bits (709), Expect = 6.1e-75
Identity = 135/167 (80.84%), Postives = 154/167 (92.22%), Query Frame = 0


Query: 1   MATATYPPPPPFYKLYKDYLQDPKSAPEPPPPIEGTYMCFGSNYTTDDVLPSLEEQGVRQ 60
           MATATYPPPPP+Y+LYKD+ ++  SAPEPPPPIEGTY+CFG NYTT+DVLPSLEEQGV Q
Sbjct: 1   MATATYPPPPPYYRLYKDFSENTDSAPEPPPPIEGTYVCFGGNYTTEDVLPSLEEQGVPQ 60

Query: 61  LYPRGPNVDYKKELRSLNRELQLHILELADILVERPSQYARRVEEISLIFKNLHHLLNSL 120
           LYP+  N+DYKKELRSLNRELQLHILELAD+LV+RPSQYA+R+ EIS IFKNLHHLLNSL
Sbjct: 61  LYPKDSNLDYKKELRSLNRELQLHILELADVLVDRPSQYAKRIGEISSIFKNLHHLLNSL 120

Query: 121 RPHQARATLIHILELQIERRRQAVEDIKRRREEAQRLLMESLETLDG 168
           RPHQARATLIHI+ELQI++R+QAVEDIKRRREEAQ LL ++  TLDG
Sbjct: 121 RPHQARATLIHIMELQIQQRKQAVEDIKRRREEAQGLLKDAFVTLDG 167

[Close]

BLAST of CSPI03G36120 vs. TAIR 10
Match: AT5G03500.2 (Mediator complex, subunit Med7 )

HSP 1 Score: 277.7 bits (709), Expect = 6.1e-75
Identity = 135/167 (80.84%), Postives = 154/167 (92.22%), Query Frame = 0


Query: 1   MATATYPPPPPFYKLYKDYLQDPKSAPEPPPPIEGTYMCFGSNYTTDDVLPSLEEQGVRQ 60
           MATATYPPPPP+Y+LYKD+ ++  SAPEPPPPIEGTY+CFG NYTT+DVLPSLEEQGV Q
Sbjct: 1   MATATYPPPPPYYRLYKDFSENTDSAPEPPPPIEGTYVCFGGNYTTEDVLPSLEEQGVPQ 60

Query: 61  LYPRGPNVDYKKELRSLNRELQLHILELADILVERPSQYARRVEEISLIFKNLHHLLNSL 120
           LYP+  N+DYKKELRSLNRELQLHILELAD+LV+RPSQYA+R+ EIS IFKNLHHLLNSL
Sbjct: 61  LYPKDSNLDYKKELRSLNRELQLHILELADVLVDRPSQYAKRIGEISSIFKNLHHLLNSL 120

Query: 121 RPHQARATLIHILELQIERRRQAVEDIKRRREEAQRLLMESLETLDG 168
           RPHQARATLIHI+ELQI++R+QAVEDIKRRREEAQ LL ++  TLDG
Sbjct: 121 RPHQARATLIHIMELQIQQRKQAVEDIKRRREEAQGLLKDAFVTLDG 167

[Close]

BLAST of CSPI03G36120 vs. TAIR 10
Match: AT5G03500.3 (Mediator complex, subunit Med7 )

HSP 1 Score: 277.7 bits (709), Expect = 6.1e-75
Identity = 135/167 (80.84%), Postives = 154/167 (92.22%), Query Frame = 0


Query: 1   MATATYPPPPPFYKLYKDYLQDPKSAPEPPPPIEGTYMCFGSNYTTDDVLPSLEEQGVRQ 60
           MATATYPPPPP+Y+LYKD+ ++  SAPEPPPPIEGTY+CFG NYTT+DVLPSLEEQGV Q
Sbjct: 1   MATATYPPPPPYYRLYKDFSENTDSAPEPPPPIEGTYVCFGGNYTTEDVLPSLEEQGVPQ 60

Query: 61  LYPRGPNVDYKKELRSLNRELQLHILELADILVERPSQYARRVEEISLIFKNLHHLLNSL 120
           LYP+  N+DYKKELRSLNRELQLHILELAD+LV+RPSQYA+R+ EIS IFKNLHHLLNSL
Sbjct: 61  LYPKDSNLDYKKELRSLNRELQLHILELADVLVDRPSQYAKRIGEISSIFKNLHHLLNSL 120

Query: 121 RPHQARATLIHILELQIERRRQAVEDIKRRREEAQRLLMESLETLDG 168
           RPHQARATLIHI+ELQI++R+QAVEDIKRRREEAQ LL ++  TLDG
Sbjct: 121 RPHQARATLIHIMELQIQQRKQAVEDIKRRREEAQGLLKDAFVTLDG 167

The following BLAST results are available for this feature:

BLAST of CSPI03G36120 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LYW3	2.7e-75	82.04	Mediator of RNA polymerase II transcription subunit 7a OS=Arabidopsis thaliana O...	[more]
Q9LZD7	8.6e-74	80.84	Mediator of RNA polymerase II transcription subunit 7b OS=Arabidopsis thaliana O...	[more]
Q55DQ5	5.5e-28	44.68	Putative mediator of RNA polymerase II transcription subunit 7 OS=Dictyostelium ...	[more]
Q17MI0	1.4e-26	39.75	Mediator of RNA polymerase II transcription subunit 7 OS=Aedes aegypti OX=7159 G...	[more]
Q3T123	3.7e-24	37.95	Mediator of RNA polymerase II transcription subunit 7 OS=Bos taurus OX=9913 GN=M...	[more]

BLAST of CSPI03G36120 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7TKB8	1.7e-88	100.00	Mediator of RNA polymerase II transcription subunit 7 OS=Cucumis melo var. makuw...	[more]
A0A0A0LBQ7	1.7e-88	100.00	Mediator of RNA polymerase II transcription subunit 7 OS=Cucumis sativus OX=3659...	[more]
A0A1S3B7S4	1.7e-88	100.00	Mediator of RNA polymerase II transcription subunit 7 OS=Cucumis melo OX=3656 GN...	[more]
A0A6J1DBY3	1.3e-85	96.43	Mediator of RNA polymerase II transcription subunit 7 OS=Momordica charantia OX=...	[more]
A0A6J1FG49	1.3e-85	96.43	Mediator of RNA polymerase II transcription subunit 7 OS=Cucurbita moschata OX=3...	[more]

BLAST of CSPI03G36120 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008443001.1	3.5e-88	100.00	PREDICTED: mediator of RNA polymerase II transcription subunit 7a-like [Cucumis ...	[more]
XP_031737683.1	2.9e-87	98.81	LOW QUALITY PROTEIN: mediator of RNA polymerase II transcription subunit 7a-like...	[more]
XP_004136548.2	3.3e-86	99.40	mediator of RNA polymerase II transcription subunit 7a [Cucumis sativus] >KAE864...	[more]
XP_022937468.1	2.8e-85	96.43	mediator of RNA polymerase II transcription subunit 7a [Cucurbita moschata] >KAG...	[more]
XP_022151348.1	2.8e-85	96.43	mediator of RNA polymerase II transcription subunit 7a-like [Momordica charantia...	[more]

BLAST of CSPI03G36120 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G03220.1	1.9e-76	82.04	Mediator complex, subunit Med7	[more]
AT5G03500.1	6.1e-75	80.84	Mediator complex, subunit Med7	[more]
AT5G03500.2	6.1e-75	80.84	Mediator complex, subunit Med7	[more]
AT5G03500.3	6.1e-75	80.84	Mediator complex, subunit Med7	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 130..164
None	No IPR available	COILS	Coil	Coil	coord: 67..87
None	No IPR available	PANTHER	PTHR21428	MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 7	coord: 2..168
IPR009244	Mediator complex, subunit Med7	PFAM	PF05983	Med7	coord: 4..157 e-value: 5.5E-45 score: 153.9
IPR044888	Mediator complex, subunit Med7 superfmaily	GENE3D	6.10.140.200		coord: 63..159 e-value: 1.5E-29 score: 103.9
IPR037212	Mediator complex, subunit Med7/Med21-like	SUPERFAMILY	140718	Mediator hinge subcomplex-like	coord: 66..157

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G36120.1	CSPI03G36120.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006357	regulation of transcription by RNA polymerase II
cellular_component	GO:0070847	core mediator complex
cellular_component	GO:0016592	mediator complex
molecular_function	GO:0003712	transcription coregulator activity