CSPI03G35440 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI03G35440
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Eukaryotic aspartyl protease family protein
Location	Chr3: 30930376 .. 30932252 (+)
RNA-Seq Expression	CSPI03G35440
Synteny	CSPI03G35440

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TACGTTCATTTGCATACCATTTTCTCTTCTCTAAAACCGCAGCTCTGCTTCCAGCTCACCCCTTTGTCCCTTCTTCCTCTCCATTTATACTTCTATTCCCCATTTCTCATCCCTTCTCATCAACATAACTCCACATTCCCTTCATTCCTTTCCAATCCATAATACCATCACACCATTTCGTTTACAACTAGGAGTAACCTTCTTCCATGGCTTCTCCTTCCCCTCTCTCTTTCTTCTACCTCCTCCTCTTCTCCTCTCTTTCCGCCATTGCCCACTCCAATCCCATCACTCTCCCTCTCAACTCTTTTCCCCACCTTTCTTCTCCAGATCCACTCCAGGCTCTCACTTTCCTCGCCTCTTCTTCCCAGACCAGAGCCCATCAAATCAAAACCCCCAAATCCAACTCTGTTTTCAAGTCCCCTCTCTCCCCCCATAGCTATGGAGCTTACTCCACTCCACTCAGCTTTGGTACTCCACAACAGACTCTGCATTTGATCTTCGATACAGGTAGTAGCCTCGTTTGGTTCCCTTGCACTTCCCGATATCTCTGTTCTGAATGTTCCTTCCCCAAAATAGATCCAACTGGAATCCCCAGATTTGTCCCCAAATTGTCCTCCTCTTCTAAGCTTGTCGGTTGCCAGAATCCCAAATGTGCCTGGATTTTTGGTCCCGATGTCAAATCTCAGTGCCGGAGTTGTAACCCCAAAACAGAGAACTGTACCCAAACTTGCCCTGCTTACGTTGTTCAATATGGTTCCGGTTCCACGGCTGGGCTTTTGCTATCAGAAACCCTTGATTTTCCTGATAAAAAAATCCCTAATTTTGTTGTTGGGTGTTCGTTTTTGTCGATCCATCAGCCCTCTGGAATCGCCGGATTTGGCCGAGGATCTGAATCGCTCCCCTCGCAAATGGGTCTCAAGAAATTCGCTTACTGCCTAGCGTCTCGGAAATTCGACGACTCGCCGCATTCCGGCCAGCTGATTTTAGATTCAACCGGCGTGAAGTCCAGCGGCCTTACCTACACCCCATTCCGGCAGAACCCCTCTGTTTCTAACAACGCTTATAAAGAATACTATTACTTAAACATACGGAAAATCATCGTCGGAAACCAGGCTGTGAAGGTTCCATACAAGTATCTAGTGCCGGGCCCCGATGGGAACGGCGGATCTATCATCGATTCCGGCTCCACCTTCACGTTTATGGACAAACCAGTCTTGGAGGTGGTGGCGCGAGAGTTCGAGAAGCAATTGGCAAATTGGACGAGAGCCACGGATGTGGAAACTCTCACTGGATTACGGCCGTGTTTCGACATTTCGAAGGAGAAATCGGTGAAGTTTCCGGAGTTGATTTTCCAATTTAAAGGTGGAGCGAAATGGGCTCTGCCGTTGAATAACTATTTCGCTTTAGTCAGTAGCTCCGGCGTGGCGTGTTTGACAGTAGTAACGCATCAGATGGAGGACGGCGGTGGCGGTGGGCCGTCTGTGATTTTAGGGGCTTTCCAGCAGCAGAATTTCTATGTGGAATATGACTTGGTGAATCAAAGATTGGGATTTCGGCAACAGACTTGCTCGTAGAAACGATGTCGTATTGGTGTATGCATGTTTATGTTTTCTAACTTTGGGTATTTGGTTGGTCGGTGGCCGGTTAAGCTACTGGTCGGAGCTACAACGGTGGTTTCAATTTGGCAGCGGCGGCGTTCAGATGAATTCAAAATCAATTTTTTTTTCACTTTCTTGTAATAATTATTCGTTGCTGTTGTAATGATATATCTCATATTTCATAATTTGAAGACAGAAAAGAATGTTAAAAAGTAATAGATGATCCCTAATAAGTTAACCTTTTTTCATTCATCATTATATATATAACGCACACACACA

mRNA sequence

TACGTTCATTTGCATACCATTTTCTCTTCTCTAAAACCGCAGCTCTGCTTCCAGCTCACCCCTTTGTCCCTTCTTCCTCTCCATTTATACTTCTATTCCCCATTTCTCATCCCTTCTCATCAACATAACTCCACATTCCCTTCATTCCTTTCCAATCCATAATACCATCACACCATTTCGTTTACAACTAGGAGTAACCTTCTTCCATGGCTTCTCCTTCCCCTCTCTCTTTCTTCTACCTCCTCCTCTTCTCCTCTCTTTCCGCCATTGCCCACTCCAATCCCATCACTCTCCCTCTCAACTCTTTTCCCCACCTTTCTTCTCCAGATCCACTCCAGGCTCTCACTTTCCTCGCCTCTTCTTCCCAGACCAGAGCCCATCAAATCAAAACCCCCAAATCCAACTCTGTTTTCAAGTCCCCTCTCTCCCCCCATAGCTATGGAGCTTACTCCACTCCACTCAGCTTTGGTACTCCACAACAGACTCTGCATTTGATCTTCGATACAGGTAGTAGCCTCGTTTGGTTCCCTTGCACTTCCCGATATCTCTGTTCTGAATGTTCCTTCCCCAAAATAGATCCAACTGGAATCCCCAGATTTGTCCCCAAATTGTCCTCCTCTTCTAAGCTTGTCGGTTGCCAGAATCCCAAATGTGCCTGGATTTTTGGTCCCGATGTCAAATCTCAGTGCCGGAGTTGTAACCCCAAAACAGAGAACTGTACCCAAACTTGCCCTGCTTACGTTGTTCAATATGGTTCCGGTTCCACGGCTGGGCTTTTGCTATCAGAAACCCTTGATTTTCCTGATAAAAAAATCCCTAATTTTGTTGTTGGGTGTTCGTTTTTGTCGATCCATCAGCCCTCTGGAATCGCCGGATTTGGCCGAGGATCTGAATCGCTCCCCTCGCAAATGGGTCTCAAGAAATTCGCTTACTGCCTAGCGTCTCGGAAATTCGACGACTCGCCGCATTCCGGCCAGCTGATTTTAGATTCAACCGGCGTGAAGTCCAGCGGCCTTACCTACACCCCATTCCGGCAGAACCCCTCTGTTTCTAACAACGCTTATAAAGAATACTATTACTTAAACATACGGAAAATCATCGTCGGAAACCAGGCTGTGAAGGTTCCATACAAGTATCTAGTGCCGGGCCCCGATGGGAACGGCGGATCTATCATCGATTCCGGCTCCACCTTCACGTTTATGGACAAACCAGTCTTGGAGGTGGTGGCGCGAGAGTTCGAGAAGCAATTGGCAAATTGGACGAGAGCCACGGATGTGGAAACTCTCACTGGATTACGGCCGTGTTTCGACATTTCGAAGGAGAAATCGGTGAAGTTTCCGGAGTTGATTTTCCAATTTAAAGGTGGAGCGAAATGGGCTCTGCCGTTGAATAACTATTTCGCTTTAGTCAGTAGCTCCGGCGTGGCGTGTTTGACAGTAGTAACGCATCAGATGGAGGACGGCGGTGGCGGTGGGCCGTCTGTGATTTTAGGGGCTTTCCAGCAGCAGAATTTCTATGTGGAATATGACTTGGTGAATCAAAGATTGGGATTTCGGCAACAGACTTGCTCGTAGAAACGATGTCGTATTGGTGTATGCATGTTTATGTTTTCTAACTTTGGGTATTTGGTTGGTCGGTGGCCGGTTAAGCTACTGGTCGGAGCTACAACGGTGGTTTCAATTTGGCAGCGGCGGCGTTCAGATGAATTCAAAATCAATTTTTTTTTCACTTTCTTGTAATAATTATTCGTTGCTGTTGTAATGATATATCTCATATTTCATAATTTGAAGACAGAAAAGAATGTTAAAAAGTAATAGATGATCCCTAATAAGTTAACCTTTTTTCATTCATCATTATATATATAACGCACACACACA

Coding sequence (CDS)

ATGGCTTCTCCTTCCCCTCTCTCTTTCTTCTACCTCCTCCTCTTCTCCTCTCTTTCCGCCATTGCCCACTCCAATCCCATCACTCTCCCTCTCAACTCTTTTCCCCACCTTTCTTCTCCAGATCCACTCCAGGCTCTCACTTTCCTCGCCTCTTCTTCCCAGACCAGAGCCCATCAAATCAAAACCCCCAAATCCAACTCTGTTTTCAAGTCCCCTCTCTCCCCCCATAGCTATGGAGCTTACTCCACTCCACTCAGCTTTGGTACTCCACAACAGACTCTGCATTTGATCTTCGATACAGGTAGTAGCCTCGTTTGGTTCCCTTGCACTTCCCGATATCTCTGTTCTGAATGTTCCTTCCCCAAAATAGATCCAACTGGAATCCCCAGATTTGTCCCCAAATTGTCCTCCTCTTCTAAGCTTGTCGGTTGCCAGAATCCCAAATGTGCCTGGATTTTTGGTCCCGATGTCAAATCTCAGTGCCGGAGTTGTAACCCCAAAACAGAGAACTGTACCCAAACTTGCCCTGCTTACGTTGTTCAATATGGTTCCGGTTCCACGGCTGGGCTTTTGCTATCAGAAACCCTTGATTTTCCTGATAAAAAAATCCCTAATTTTGTTGTTGGGTGTTCGTTTTTGTCGATCCATCAGCCCTCTGGAATCGCCGGATTTGGCCGAGGATCTGAATCGCTCCCCTCGCAAATGGGTCTCAAGAAATTCGCTTACTGCCTAGCGTCTCGGAAATTCGACGACTCGCCGCATTCCGGCCAGCTGATTTTAGATTCAACCGGCGTGAAGTCCAGCGGCCTTACCTACACCCCATTCCGGCAGAACCCCTCTGTTTCTAACAACGCTTATAAAGAATACTATTACTTAAACATACGGAAAATCATCGTCGGAAACCAGGCTGTGAAGGTTCCATACAAGTATCTAGTGCCGGGCCCCGATGGGAACGGCGGATCTATCATCGATTCCGGCTCCACCTTCACGTTTATGGACAAACCAGTCTTGGAGGTGGTGGCGCGAGAGTTCGAGAAGCAATTGGCAAATTGGACGAGAGCCACGGATGTGGAAACTCTCACTGGATTACGGCCGTGTTTCGACATTTCGAAGGAGAAATCGGTGAAGTTTCCGGAGTTGATTTTCCAATTTAAAGGTGGAGCGAAATGGGCTCTGCCGTTGAATAACTATTTCGCTTTAGTCAGTAGCTCCGGCGTGGCGTGTTTGACAGTAGTAACGCATCAGATGGAGGACGGCGGTGGCGGTGGGCCGTCTGTGATTTTAGGGGCTTTCCAGCAGCAGAATTTCTATGTGGAATATGACTTGGTGAATCAAAGATTGGGATTTCGGCAACAGACTTGCTCGTAG

Protein sequence

MASPSPLSFFYLLLFSSLSAIAHSNPITLPLNSFPHLSSPDPLQALTFLASSSQTRAHQIKTPKSNSVFKSPLSPHSYGAYSTPLSFGTPQQTLHLIFDTGSSLVWFPCTSRYLCSECSFPKIDPTGIPRFVPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAYVVQYGSGSTAGLLLSETLDFPDKKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLKKFAYCLASRKFDDSPHSGQLILDSTGVKSSGLTYTPFRQNPSVSNNAYKEYYYLNIRKIIVGNQAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVLEVVAREFEKQLANWTRATDVETLTGLRPCFDISKEKSVKFPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTHQMEDGGGGGPSVILGAFQQQNFYVEYDLVNQRLGFRQQTCS*

Homology

[Close]

BLAST of CSPI03G35440 vs. ExPASy Swiss-Prot
Match: Q940R4 (Probable aspartyl protease At4g16563 OS=Arabidopsis thaliana OX=3702 GN=At4g16563 PE=2 SV=1)

HSP 1 Score: 194.1 bits (492), Expect = 3.4e-48
Identity = 149/501 (29.74%), Postives = 224/501 (44.71%), Query Frame = 0


Query: 7   LSFFYLLLFSSLSA-----IAHSNPITLPLNSFPHLSSPDPLQALTFLASSSQTRAHQIK 66
           L +++    SSLS      ++HS      L++  H SSP  L   +   SS++ R H  K
Sbjct: 14  LQYYFHFSVSSLSTPLLLHLSHS------LSTSKHSSSPLHLLKSSSSRSSARFRRHHHK 73

Query: 67  TPKSNSVFKSPLSPHSYGAYSTPLSFGTPQQTLHLIFDTGSSLVWFPCTSRYLCSECSFP 126
             +       P+S  S   Y   LS G+    + L  DTGS LVWFPC   + C  C   
Sbjct: 74  --QQQQQLSLPIS--SGSDYLISLSVGSSSSAVSLYLDTGSDLVWFPCRP-FTCILCESK 133

Query: 127 KIDPTGIPRFVPKLSSSSKLVGCQNPKCAWIFGPDVKSQ-CRSCN-----PKTENCTQT- 186
            + P+        LSSS+  V C +P C+        S  C   N      +T +C  + 
Sbjct: 134 PLPPSP----PSSLSSSATTVSCSSPSCSAAHSSLPSSDLCAISNCPLDFIETGDCNTSS 193

Query: 187 --CPAYVVQYGSGSTAGLLLSETLDFPDKKIPNFVVGCSFLSIHQPSGIAGFGRGSESLP 246
             CP +   YG GS    L S++L  P   + NF  GC+  ++ +P G+AGFGRG  SLP
Sbjct: 194 YPCPPFYYAYGDGSLVAKLYSDSLSLPSVSVSNFTFGCAHTTLAEPIGVAGFGRGRLSLP 253

Query: 247 SQMGL------KKFAYCLASRKFDDS--PHSGQLIL--------------------DSTG 306
           +Q+ +        F+YCL S  FD         LIL                    D   
Sbjct: 254 AQLAVHSPHLGNSFSYCLVSHSFDSDRVRRPSPLILGRFVDKKEKRVGTTDDHDDGDDEK 313

Query: 307 VKSSGLTYTPFRQNPSVSNNAYKEYYYLNIRKIIVGNQAVKVPYKYLVPGPDGNGGSIID 366
            K +   +T   +NP      +  +Y ++++ I +G + +  P        +G GG ++D
Sbjct: 314 KKKNEFVFTEMLENPK-----HPYFYSVSLQGISIGKRNIPAPAMLRRIDKNGGGGVVVD 373

Query: 367 SGSTFTFMDKPVLEVVAREFEKQLAN-WTRATDVETLTGLRPCFDISKEKSVKFPELIFQ 426
           SG+TFT +       V  EF+ ++     RA  VE  +G+ PC+ ++  ++VK P L+  
Sbjct: 374 SGTTFTMLPAKFYNSVVEEFDSRVGRVHERADRVEPSSGMSPCYYLN--QTVKVPALVLH 433

Query: 427 FKGG-AKWALPLNNYFALVSSSG--------VACLTVVTHQMEDGGGGGPSVILGAFQQQ 456
           F G  +   LP  NYF      G        + CL ++    E    GG   ILG +QQQ
Sbjct: 434 FAGNRSSVTLPRRNYFYEFMDGGDGKEEKRKIGCLMLMNGGDESELRGGTGAILGNYQQQ 492

[Close]

BLAST of CSPI03G35440 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 159.1 bits (401), Expect = 1.2e-37
Identity = 143/465 (30.75%), Postives = 197/465 (42.37%), Query Frame = 0


Query: 15  FSSLSAIAHSNPITLPLNSFPHLSS---PDP-----LQALTFLASSSQTRAHQI------ 74
           F S S    S+ ITL L+    LSS   PD      LQ  +    S  T A QI      
Sbjct: 60  FESGSDSESSSSITLNLDHIDALSSNKTPDELFSSRLQRDSRRVKSIATLAAQIPGRNVT 119

Query: 75  KTPKSNSVFKSPLSPHSYGA--YSTPLSFGTPQQTLHLIFDTGSSLVWFPCTSRYLCSEC 134
             P+      S +S  S G+  Y T L  GTP + ++++ DTGS +VW  C     C  C
Sbjct: 120 HAPRPGGFSSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAP---CRRC 179

Query: 135 SFPKIDPTGIPRFVPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY 194
            + + DP     F P+ S +   + C +P C        +     CN + + C      Y
Sbjct: 180 -YSQSDPI----FDPRKSKTYATIPCSSPHCR-------RLDSAGCNTRRKTC-----LY 239

Query: 195 VVQYGSGS-TAGLLLSETLDFPDKKIPNFVVGCSFLS---IHQPSGIAGFGRGSESLPSQ 254
            V YG GS T G   +ETL F   ++    +GC   +       +G+ G G+G  S P Q
Sbjct: 240 QVSYGDGSFTVGDFSTETLTFRRNRVKGVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQ 299

Query: 255 MGLK---KFAYCLASRKFDDSPHSGQLILDSTGVKSSGLTYTPFRQNPSVSNNAYKEYYY 314
            G +   KF+YCL  R     P S   ++      S    +TP   NP +       +YY
Sbjct: 300 TGHRFNQKFSYCLVDRSASSKPSS---VVFGNAAVSRIARFTPLLSNPKLDT-----FYY 359

Query: 315 LNIRKIIVGNQAVK-VPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVLEVVAREFEKQLAN 374
           + +  I VG   V  V          GNGG IIDSG++ T + +P    +   F      
Sbjct: 360 VGLLGISVGGTRVPGVTASLFKLDQIGNGGVIIDSGTSVTRLIRPAYIAMRDAFRVGAKT 419

Query: 375 WTRATDVETLTGLRPCFDISKEKSVKFPELIFQFKGGAKWALPLNNYFALVSSSGVACLT 434
             RA D         CFD+S    VK P ++  F+ GA  +LP  NY   V ++G  C  
Sbjct: 420 LKRAPDFSLFD---TCFDLSNMNEVKVPTVVLHFR-GADVSLPATNYLIPVDTNGKFCFA 479

Query: 435 VVTHQMEDGGGGGPSVILGAFQQQNFYVEYDLVNQRLGFRQQTCS 456
                   G  GG S+I G  QQQ F V YDL + R+GF    C+
Sbjct: 480 FA------GTMGGLSII-GNIQQQGFRVVYDLASSRVGFAPGGCA 485

[Close]

BLAST of CSPI03G35440 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 149.4 bits (376), Expect = 9.6e-35
Identity = 122/383 (31.85%), Postives = 169/383 (44.13%), Query Frame = 0


Query: 79  GAYSTPLSFGTPQQTLHLIFDTGSSLVWFPCTSRYLCSECSFPKIDPTGIPRFVPKLSSS 138
           G Y   ++ GTP  +   I DTGS L+W  C     C++C      PT  P F P+ SSS
Sbjct: 94  GEYLMNVAIGTPDSSFSAIMDTGSDLIWTQCEP---CTQCF---SQPT--PIFNPQDSSS 153

Query: 139 SKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAYVVQYGSGSTA-GLLLSETLD 198
              + C++  C      D+ S         E C      Y   YG GST  G + +ET  
Sbjct: 154 FSTLPCESQYC-----QDLPS---------ETCNNNECQYTYGYGDGSTTQGYMATETFT 213

Query: 199 FPDKKIPNFVVGC----SFLSIHQPSGIAGFGRGSESLPSQMGLKKFAYCLASRKFDDSP 258
           F    +PN   GC            +G+ G G G  SLPSQ+G+ +F+YC+ S     SP
Sbjct: 214 FETSSVPNIAFGCGEDNQGFGQGNGAGLIGMGWGPLSLPSQLGVGQFSYCMTSYG-SSSP 273

Query: 259 HSGQLILDSTGVKSSGLTYTPFRQNPSVSNNAYKEYYYLNIRKIIVGNQAVKVPYKYLVP 318
            +  L   ++GV     + T       + ++    YYY+ ++ I VG   + +P      
Sbjct: 274 STLALGSAASGVPEGSPSTT------LIHSSLNPTYYYITLQGITVGGDNLGIPSSTFQL 333

Query: 319 GPDGNGGSIIDSGSTFTFMDKPVLEVVAREFEKQLANWTRATDVETLTGLRPCFDISKEK 378
             DG GG IIDSG+T T++ +     VA+ F  Q+      T  E+ +GL  CF    + 
Sbjct: 334 QDDGTGGMIIDSGTTLTYLPQDAYNAVAQAFTDQI---NLPTVDESSSGLSTCFQQPSDG 393

Query: 379 S-VKFPELIFQFKGGAKWALPLNNYFALVS-SSGVACLTVVTHQMEDGGGGGPSVILGAF 438
           S V+ PE+  QF GG    L L     L+S + GV CL      M      G S I G  
Sbjct: 394 STVQVPEISMQFDGG---VLNLGEQNILISPAEGVICLA-----MGSSSQLGIS-IFGNI 435

Query: 439 QQQNFYVEYDLVNQRLGFRQQTC 455
           QQQ   V YDL N  + F    C
Sbjct: 454 QQQETQVLYDLQNLAVSFVPTQC 435

[Close]

BLAST of CSPI03G35440 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 145.6 bits (366), Expect = 1.4e-33
Identity = 113/387 (29.20%), Postives = 160/387 (41.34%), Query Frame = 0


Query: 79  GAYSTPLSFGTPQQTLHLIFDTGSSLVWFPCTSRYLCSECSFPKIDPTGIPRFVPKLSSS 138
           G Y   LS GTP Q    I DTGS L+W  C     C   S P  +P G        SSS
Sbjct: 93  GEYLMNLSIGTPAQPFSAIMDTGSDLIWTQCQPCTQCFNQSTPIFNPQG--------SSS 152

Query: 139 SKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAYVVQYGSGS-TAGLLLSETLD 198
              + C +  C  +  P               C+     Y   YG GS T G + +ETL 
Sbjct: 153 FSTLPCSSQLCQALSSP--------------TCSNNFCQYTYGYGDGSETQGSMGTETLT 212

Query: 199 FPDKKIPNFVVGC----SFLSIHQPSGIAGFGRGSESLPSQMGLKKFAYCLASRKFDDSP 258
           F    IPN   GC            +G+ G GRG  SLPSQ+ + KF+YC+       S 
Sbjct: 213 FGSVSIPNITFGCGENNQGFGQGNGAGLVGMGRGPLSLPSQLDVTKFSYCMT--PIGSST 272

Query: 259 HSGQLILDSTGVKSSGLTYTPFRQNPSVSNNAYKEYYYLNIRKIIVGNQAVKV-PYKYLV 318
            S  L+       ++G   T   Q+  +       +YY+ +  + VG+  + + P  + +
Sbjct: 273 PSNLLLGSLANSVTAGSPNTTLIQSSQIPT-----FYYITLNGLSVGSTRLPIDPSAFAL 332

Query: 319 PGPDGNGGSIIDSGSTFTFMDKPVLEVVAREFEKQLANWTRATDVETLTGLRPCFDI--- 378
              +G GG IIDSG+T T+      + V +EF  Q+       ++  + G    FD+   
Sbjct: 333 NSNNGTGGIIIDSGTTLTYFVNNAYQSVRQEFISQI-------NLPVVNGSSSGFDLCFQ 392

Query: 379 --SKEKSVKFPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTHQMEDGGGGGPSVI 438
             S   +++ P  +  F GG    LP  NYF +  S+G+ CL +       G       I
Sbjct: 393 TPSDPSNLQIPTFVMHFDGG-DLELPSENYF-ISPSNGLICLAM-------GSSSQGMSI 434

Query: 439 LGAFQQQNFYVEYDLVNQRLGFRQQTC 455
            G  QQQN  V YD  N  + F    C
Sbjct: 453 FGNIQQQNMLVVYDTGNSVVSFASAQC 434

[Close]

BLAST of CSPI03G35440 vs. ExPASy Swiss-Prot
Match: Q6F4N5 (Aspartyl protease 25 OS=Oryza sativa subsp. japonica OX=39947 GN=AP25 PE=2 SV=1)

HSP 1 Score: 139.8 bits (351), Expect = 7.6e-32
Identity = 127/468 (27.14%), Postives = 200/468 (42.74%), Query Frame = 0


Query: 1   MASPSPLSFFYLLLFSSLSAIAHSNPITLPLNSFPHLSSPDPLQALTFLASSSQTRAHQI 60
           MA+ + +    LLL ++++A A      L +    H SSP PL+++  LA     R   +
Sbjct: 1   MAATTTIPLLLLLLAATVAAAA----AELSVYHNVHPSSPSPLESIIALARDDDARLLFL 60

Query: 61  KTPKSNS-VFKSPL-SPHSYGAYSTPLSFGTPQQTLHLIFDTGSSLVWFPCTSRYLCSEC 120
            +  + + V  +P+ S  +  +Y      G+P Q L L  DT +   W  C+    C  C
Sbjct: 61  SSKAATAGVSSAPVASGQAPPSYVVRAGLGSPSQQLLLALDTSADATWAHCSP---CGTC 120

Query: 121 SFPKIDPTGIPRFVPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY 180
               +       F P  SSS   + C +  C    G    +     +      T    A+
Sbjct: 121 PSSSL-------FAPANSSSYASLPCSSSWCPLFQGQACPAPQGGGDAAPPPATLPTCAF 180

Query: 181 VVQYGSGSTAGLLLSETLDFPDKKIPNFVVGCSFLSIHQPS------GIAGFGRGSESLP 240
              +   S    L S+TL      IPN+  GC   S+  P+      G+ G GRG  +L 
Sbjct: 181 SKPFADASFQAALASDTLRLGKDAIPNYTFGC-VSSVTGPTTNMPRQGLLGLGRGPMALL 240

Query: 241 SQMGL---KKFAYCLASRKFDDSPHSGQLILDSTGVKSSGLTYTPFRQNPSVSNNAYKEY 300
           SQ G      F+YCL S  +     SG L L + G +   + YTP  +NP  S+      
Sbjct: 241 SQAGSLYNGVFSYCLPS--YRSYYFSGSLRLGAGGGQPRSVRYTPMLRNPHRSS-----L 300

Query: 301 YYLNIRKIIVGNQAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVLEVVAREFEKQLA 360
           YY+N+  + VG+  VKVP            G+++DSG+  T    PV   +  EF +Q+A
Sbjct: 301 YYVNVTGLSVGHAWVKVPAGSFAFDAATGAGTVVDSGTVITRWTAPVYAALREEFRRQVA 360

Query: 361 NWTRATDVETLTGLRPCFDISKEKSVKFPELIFQFKGGAKWALPLNNYFALVSSSGVACL 420
                +   +L     CF+  +  +   P +     GG   ALP+ N     S++ +ACL
Sbjct: 361 ---APSGYTSLGAFDTCFNTDEVAAGGAPAVTVHMDGGVDLALPMENTLIHSSATPLACL 420

Query: 421 TVVTHQMEDGGGGGPSV--ILGAFQQQNFYVEYDLVNQRLGFRQQTCS 456
                 M +      SV  ++   QQQN  V +D+ N R+GF +++C+
Sbjct: 421 A-----MAEAPQNVNSVVNVIANLQQQNIRVVFDVANSRVGFAKESCN 438

[Close]

BLAST of CSPI03G35440 vs. ExPASy TrEMBL
Match: A0A0A0LBI9 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G778440 PE=3 SV=1)

HSP 1 Score: 926.4 bits (2393), Expect = 4.6e-266
Identity = 453/457 (99.12%), Postives = 455/457 (99.56%), Query Frame = 0


Query: 1   MASPSPLSFFYLLLFSSLSAIAHSNPITLPLNSFPHLSSPDPLQALTFLASSSQTRAHQI 60
           MASPSPLSFFYLLLFSSLSAIAHSNPITLPLNSFPHLSSPDPLQALTFLASSSQTRAHQI
Sbjct: 1   MASPSPLSFFYLLLFSSLSAIAHSNPITLPLNSFPHLSSPDPLQALTFLASSSQTRAHQI 60

Query: 61  KTPKSNSVFKSPLSPHSYGAYSTPLSFGTPQQTLHLIFDTGSSLVWFPCTSRYLCSECSF 120
           KTPKSNSVFKSPLSPHSYGAYSTPLSFGTPQQTLHLIFDTGSSLVWFPCTSRYLCSECSF
Sbjct: 61  KTPKSNSVFKSPLSPHSYGAYSTPLSFGTPQQTLHLIFDTGSSLVWFPCTSRYLCSECSF 120

Query: 121 PKIDPTGIPRFVPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAYVV 180
           PKIDPTGIPRFVPKLSSSSKLVGCQNPKC+WIFGPDVKSQCRSCNPKTENCTQTCPAYVV
Sbjct: 121 PKIDPTGIPRFVPKLSSSSKLVGCQNPKCSWIFGPDVKSQCRSCNPKTENCTQTCPAYVV 180

Query: 181 QYGSGSTAGLLLSETLDFPDKKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLKKF 240
           QYGSGSTAGLLLSETLDFPDKKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLKKF
Sbjct: 181 QYGSGSTAGLLLSETLDFPDKKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLKKF 240

Query: 241 AYCLASRKFDDSPHSGQLILDSTGVKSSGLTYTPFRQNPSVSNNAYKEYYYLNIRKIIVG 300
           AYCLASRKFDDSPHSGQLILDSTGVKSSGLTYTPFRQNPSVSNNAYKEYYYLNIRKIIVG
Sbjct: 241 AYCLASRKFDDSPHSGQLILDSTGVKSSGLTYTPFRQNPSVSNNAYKEYYYLNIRKIIVG 300

Query: 301 NQAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVLEVVAREFEKQLANWTRATDVETL 360
           NQAVKVPYK+LVPGPDGNGGSIIDSGSTFTFMDKPVLEVVAREFEKQLANWTRATDVETL
Sbjct: 301 NQAVKVPYKFLVPGPDGNGGSIIDSGSTFTFMDKPVLEVVAREFEKQLANWTRATDVETL 360

Query: 361 TGLRPCFDISKEKSVKFPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTHQMED-- 420
           TGLRPCFDISKEKSVKFPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTHQMED  
Sbjct: 361 TGLRPCFDISKEKSVKFPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTHQMEDGG 420

Query: 421 GGGGGPSVILGAFQQQNFYVEYDLVNQRLGFRQQTCS 456
           GGGGGPSVILGAFQQQNFYVEYDLVNQRLGFRQQTCS
Sbjct: 421 GGGGGPSVILGAFQQQNFYVEYDLVNQRLGFRQQTCS 457

[Close]

BLAST of CSPI03G35440 vs. ExPASy TrEMBL
Match: A0A5A7TRK2 (Aspartic proteinase nepenthesin-2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G004410 PE=3 SV=1)

HSP 1 Score: 882.1 bits (2278), Expect = 1.0e-252
Identity = 428/455 (94.07%), Postives = 446/455 (98.02%), Query Frame = 0


Query: 1   MASPSPLSFFYLLLFSSLSAIAHSNPITLPLNSFPHLSSPDPLQALTFLASSSQTRAHQI 60
           MASPSPLSFFY+LLFSSLSAI++SNPITLPLNS PHLSS DPLQALTFLAS+S+ RAH+I
Sbjct: 1   MASPSPLSFFYILLFSSLSAISNSNPITLPLNSSPHLSSSDPLQALTFLASASKNRAHRI 60

Query: 61  KTPKSNSVFKSPLSPHSYGAYSTPLSFGTPQQTLHLIFDTGSSLVWFPCTSRYLCSECSF 120
           KTPKSNSV KSPLSPHSYGAYSTPLSFGTPQQTLHLIFDTGSSLVWFPCTSRYLC+ECSF
Sbjct: 61  KTPKSNSVSKSPLSPHSYGAYSTPLSFGTPQQTLHLIFDTGSSLVWFPCTSRYLCTECSF 120

Query: 121 PKIDPTGIPRFVPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAYVV 180
           PKIDPTGIPRFVPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAYVV
Sbjct: 121 PKIDPTGIPRFVPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAYVV 180

Query: 181 QYGSGSTAGLLLSETLDFPDKKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLKKF 240
           QYGSGSTAGLLLSETLDFP+KKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLKKF
Sbjct: 181 QYGSGSTAGLLLSETLDFPNKKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLKKF 240

Query: 241 AYCLASRKFDDSPHSGQLILDSTGVKSSGLTYTPFRQNPSVSNNAYKEYYYLNIRKIIVG 300
           AYCLASRKFDDS HSGQLILDS+GVK+SGLTYT FRQNPSVSN+AYKEYYYLNIRKIIVG
Sbjct: 241 AYCLASRKFDDSAHSGQLILDSSGVKTSGLTYTSFRQNPSVSNHAYKEYYYLNIRKIIVG 300

Query: 301 NQAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVLEVVAREFEKQLANWTRATDVETL 360
           NQAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVL+VVA+EFEKQLAN TRATDVETL
Sbjct: 301 NQAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVLDVVAQEFEKQLANRTRATDVETL 360

Query: 361 TGLRPCFDISKEKSVKFPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTHQMEDGG 420
           TGLRPCFD+SKEKSV+FPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTH  EDGG
Sbjct: 361 TGLRPCFDVSKEKSVEFPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTHNTEDGG 420

Query: 421 GGGPSVILGAFQQQNFYVEYDLVNQRLGFRQQTCS 456
           GGGPSVILGAFQQQNFYVEYDLVN+RLGFR+QTC+
Sbjct: 421 GGGPSVILGAFQQQNFYVEYDLVNERLGFRKQTCT 455

[Close]

BLAST of CSPI03G35440 vs. ExPASy TrEMBL
Match: A0A1S3B6B5 (aspartic proteinase nepenthesin-2 OS=Cucumis melo OX=3656 GN=LOC103486666 PE=3 SV=1)

HSP 1 Score: 882.1 bits (2278), Expect = 1.0e-252
Identity = 428/455 (94.07%), Postives = 446/455 (98.02%), Query Frame = 0


Query: 1   MASPSPLSFFYLLLFSSLSAIAHSNPITLPLNSFPHLSSPDPLQALTFLASSSQTRAHQI 60
           MASPSPLSFFY+LLFSSLSAI++SNPITLPLNS PHLSS DPLQALTFLAS+S+ RAH+I
Sbjct: 1   MASPSPLSFFYILLFSSLSAISNSNPITLPLNSSPHLSSSDPLQALTFLASASKNRAHRI 60

Query: 61  KTPKSNSVFKSPLSPHSYGAYSTPLSFGTPQQTLHLIFDTGSSLVWFPCTSRYLCSECSF 120
           KTPKSNSV KSPLSPHSYGAYSTPLSFGTPQQTLHLIFDTGSSLVWFPCTSRYLC+ECSF
Sbjct: 61  KTPKSNSVSKSPLSPHSYGAYSTPLSFGTPQQTLHLIFDTGSSLVWFPCTSRYLCTECSF 120

Query: 121 PKIDPTGIPRFVPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAYVV 180
           PKIDPTGIPRFVPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAYVV
Sbjct: 121 PKIDPTGIPRFVPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAYVV 180

Query: 181 QYGSGSTAGLLLSETLDFPDKKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLKKF 240
           QYGSGSTAGLLLSETLDFP+KKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLKKF
Sbjct: 181 QYGSGSTAGLLLSETLDFPNKKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLKKF 240

Query: 241 AYCLASRKFDDSPHSGQLILDSTGVKSSGLTYTPFRQNPSVSNNAYKEYYYLNIRKIIVG 300
           AYCLASRKFDDS HSGQLILDS+GVK+SGLTYT FRQNPSVSN+AYKEYYYLNIRKIIVG
Sbjct: 241 AYCLASRKFDDSAHSGQLILDSSGVKTSGLTYTSFRQNPSVSNHAYKEYYYLNIRKIIVG 300

Query: 301 NQAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVLEVVAREFEKQLANWTRATDVETL 360
           NQAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVL+VVA+EFEKQLAN TRATDVETL
Sbjct: 301 NQAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVLDVVAQEFEKQLANRTRATDVETL 360

Query: 361 TGLRPCFDISKEKSVKFPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTHQMEDGG 420
           TGLRPCFD+SKEKSV+FPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTH  EDGG
Sbjct: 361 TGLRPCFDVSKEKSVEFPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTHNTEDGG 420

Query: 421 GGGPSVILGAFQQQNFYVEYDLVNQRLGFRQQTCS 456
           GGGPSVILGAFQQQNFYVEYDLVN+RLGFR+QTC+
Sbjct: 421 GGGPSVILGAFQQQNFYVEYDLVNERLGFRKQTCT 455

[Close]

BLAST of CSPI03G35440 vs. ExPASy TrEMBL
Match: A0A6J1IXY3 (probable aspartyl protease At4g16563 OS=Cucurbita maxima OX=3661 GN=LOC111481640 PE=3 SV=1)

HSP 1 Score: 820.8 bits (2119), Expect = 2.7e-234
Identity = 398/457 (87.09%), Postives = 423/457 (92.56%), Query Frame = 0


Query: 1   MASPSPLSFFYLLLFSSLSAIAHSNPITLPLNSFPHLSSPDPLQALTFLASSSQTRAHQI 60
           MA P PL FFY+LL SS+SAIA +NPIT+PL+SFPH SS DPLQ L FLAS+SQ RAHQI
Sbjct: 1   MAPPPPLCFFYILLVSSVSAIADTNPITIPLSSFPHHSSSDPLQTLNFLASASQNRAHQI 60

Query: 61  KTPK--SNSVFKSPLSPHSYGAYSTPLSFGTPQQTLHLIFDTGSSLVWFPCTSRYLCSEC 120
           K PK  SNSV KSPLSPHSYGAYSTPLSFGTP QTLHLIFDTGSSLVW PCTS+YLCSEC
Sbjct: 61  KAPKSESNSVSKSPLSPHSYGAYSTPLSFGTPPQTLHLIFDTGSSLVWLPCTSKYLCSEC 120

Query: 121 SFPKIDPTGIPRFVPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY 180
           SFPKIDP GIPRF+PKLSS+SKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY
Sbjct: 121 SFPKIDPAGIPRFIPKLSSTSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY 180

Query: 181 VVQYGSGSTAGLLLSETLDFPDKKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240
           VVQYGSGSTAGLLLSETLDFPDKK  NFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK
Sbjct: 181 VVQYGSGSTAGLLLSETLDFPDKKFTNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240

Query: 241 KFAYCLASRKFDDSPHSGQLILDSTGVKSSGLTYTPFRQNPSVSNNAYKEYYYLNIRKII 300
           KFAYCLASRKFDDSPH+G+LILDS+G K+SGL+YTPFRQNPSVSN+AYKEYYYL IRKI 
Sbjct: 241 KFAYCLASRKFDDSPHAGELILDSSGAKTSGLSYTPFRQNPSVSNHAYKEYYYLTIRKIF 300

Query: 301 VGNQAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVLEVVAREFEKQLANWTRATDVE 360
           VG +AVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPV E VA+E EKQLAN TRATDVE
Sbjct: 301 VGKKAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEIEKQLANRTRATDVE 360

Query: 361 TLTGLRPCFDISKEKSVKFPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTHQMED 420
           +LTGLRPCFDISK+KSV+FPEL FQ KGGAKW LPL+NYFALVSSSGVACLTVVTH+  D
Sbjct: 361 SLTGLRPCFDISKDKSVEFPELTFQLKGGAKWGLPLSNYFALVSSSGVACLTVVTHKTAD 420

Query: 421 GGGGGPSVILGAFQQQNFYVEYDLVNQRLGFRQQTCS 456
             GGGPS+ILGAFQQQNFYVEYDLVNQ++GFRQQTCS
Sbjct: 421 -SGGGPSIILGAFQQQNFYVEYDLVNQKIGFRQQTCS 456

[Close]

BLAST of CSPI03G35440 vs. ExPASy TrEMBL
Match: A0A6J1F3G5 (probable aspartyl protease At4g16563 OS=Cucurbita moschata OX=3662 GN=LOC111441834 PE=3 SV=1)

HSP 1 Score: 819.3 bits (2115), Expect = 7.9e-234
Identity = 399/457 (87.31%), Postives = 423/457 (92.56%), Query Frame = 0


Query: 1   MASPSPLSFFYLLLFSSLSAIAHSNPITLPLNSFPHLSSPDPLQALTFLASSSQTRAHQI 60
           MA P PL FFY+LL SS+SAIA +NPITLPL+SFPH SS DPLQ L FLAS+SQ RAHQI
Sbjct: 1   MAPPPPLCFFYILLLSSVSAIADTNPITLPLSSFPHHSSSDPLQTLNFLASASQNRAHQI 60

Query: 61  KTP--KSNSVFKSPLSPHSYGAYSTPLSFGTPQQTLHLIFDTGSSLVWFPCTSRYLCSEC 120
           K P  KSNSV KSPLSPHSYGAYSTPLSFGTP QTLHLIFDTGSSLVW PCTS+YLCSEC
Sbjct: 61  KAPKSKSNSVSKSPLSPHSYGAYSTPLSFGTPSQTLHLIFDTGSSLVWLPCTSKYLCSEC 120

Query: 121 SFPKIDPTGIPRFVPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY 180
           SFPKIDP  IPRF+PKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY
Sbjct: 121 SFPKIDPARIPRFIPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY 180

Query: 181 VVQYGSGSTAGLLLSETLDFPDKKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240
           VVQYGSGSTAGLLLSETLDFP+KKI NFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK
Sbjct: 181 VVQYGSGSTAGLLLSETLDFPNKKITNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240

Query: 241 KFAYCLASRKFDDSPHSGQLILDSTGVKSSGLTYTPFRQNPSVSNNAYKEYYYLNIRKII 300
           KFAYCLASRKFDDSPH+G+LILDS+G K+SGLTYTPFRQNPSVSN+AYKEYYYL IRKI 
Sbjct: 241 KFAYCLASRKFDDSPHAGELILDSSGAKTSGLTYTPFRQNPSVSNHAYKEYYYLTIRKIF 300

Query: 301 VGNQAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVLEVVAREFEKQLANWTRATDVE 360
           VGN+AVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPV E VA+E EKQLAN TRATDVE
Sbjct: 301 VGNKAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEIEKQLANRTRATDVE 360

Query: 361 TLTGLRPCFDISKEKSVKFPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTHQMED 420
           +LTGLRPCFDISK+KSV+FPEL F  KGGAKWA PL+NYFALVSSSGVACLTVVTH+  +
Sbjct: 361 SLTGLRPCFDISKDKSVEFPELTFHLKGGAKWAPPLSNYFALVSSSGVACLTVVTHKAAE 420

Query: 421 GGGGGPSVILGAFQQQNFYVEYDLVNQRLGFRQQTCS 456
             GGGPS+ILGAFQQQNFYVEYDLVNQ++GFRQQTCS
Sbjct: 421 -SGGGPSIILGAFQQQNFYVEYDLVNQKIGFRQQTCS 456

[Close]

BLAST of CSPI03G35440 vs. NCBI nr
Match: XP_004136706.1 (probable aspartyl protease At4g16563 [Cucumis sativus] >KGN59188.1 hypothetical protein Csa_002380 [Cucumis sativus])

HSP 1 Score: 926.4 bits (2393), Expect = 9.5e-266
Identity = 453/457 (99.12%), Postives = 455/457 (99.56%), Query Frame = 0


Query: 1   MASPSPLSFFYLLLFSSLSAIAHSNPITLPLNSFPHLSSPDPLQALTFLASSSQTRAHQI 60
           MASPSPLSFFYLLLFSSLSAIAHSNPITLPLNSFPHLSSPDPLQALTFLASSSQTRAHQI
Sbjct: 1   MASPSPLSFFYLLLFSSLSAIAHSNPITLPLNSFPHLSSPDPLQALTFLASSSQTRAHQI 60

Query: 61  KTPKSNSVFKSPLSPHSYGAYSTPLSFGTPQQTLHLIFDTGSSLVWFPCTSRYLCSECSF 120
           KTPKSNSVFKSPLSPHSYGAYSTPLSFGTPQQTLHLIFDTGSSLVWFPCTSRYLCSECSF
Sbjct: 61  KTPKSNSVFKSPLSPHSYGAYSTPLSFGTPQQTLHLIFDTGSSLVWFPCTSRYLCSECSF 120

Query: 121 PKIDPTGIPRFVPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAYVV 180
           PKIDPTGIPRFVPKLSSSSKLVGCQNPKC+WIFGPDVKSQCRSCNPKTENCTQTCPAYVV
Sbjct: 121 PKIDPTGIPRFVPKLSSSSKLVGCQNPKCSWIFGPDVKSQCRSCNPKTENCTQTCPAYVV 180

Query: 181 QYGSGSTAGLLLSETLDFPDKKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLKKF 240
           QYGSGSTAGLLLSETLDFPDKKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLKKF
Sbjct: 181 QYGSGSTAGLLLSETLDFPDKKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLKKF 240

Query: 241 AYCLASRKFDDSPHSGQLILDSTGVKSSGLTYTPFRQNPSVSNNAYKEYYYLNIRKIIVG 300
           AYCLASRKFDDSPHSGQLILDSTGVKSSGLTYTPFRQNPSVSNNAYKEYYYLNIRKIIVG
Sbjct: 241 AYCLASRKFDDSPHSGQLILDSTGVKSSGLTYTPFRQNPSVSNNAYKEYYYLNIRKIIVG 300

Query: 301 NQAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVLEVVAREFEKQLANWTRATDVETL 360
           NQAVKVPYK+LVPGPDGNGGSIIDSGSTFTFMDKPVLEVVAREFEKQLANWTRATDVETL
Sbjct: 301 NQAVKVPYKFLVPGPDGNGGSIIDSGSTFTFMDKPVLEVVAREFEKQLANWTRATDVETL 360

Query: 361 TGLRPCFDISKEKSVKFPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTHQMED-- 420
           TGLRPCFDISKEKSVKFPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTHQMED  
Sbjct: 361 TGLRPCFDISKEKSVKFPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTHQMEDGG 420

Query: 421 GGGGGPSVILGAFQQQNFYVEYDLVNQRLGFRQQTCS 456
           GGGGGPSVILGAFQQQNFYVEYDLVNQRLGFRQQTCS
Sbjct: 421 GGGGGPSVILGAFQQQNFYVEYDLVNQRLGFRQQTCS 457

[Close]

BLAST of CSPI03G35440 vs. NCBI nr
Match: XP_008442902.1 (PREDICTED: aspartic proteinase nepenthesin-2 [Cucumis melo] >KAA0043829.1 aspartic proteinase nepenthesin-2 [Cucumis melo var. makuwa] >TYK25303.1 aspartic proteinase nepenthesin-2 [Cucumis melo var. makuwa])

HSP 1 Score: 882.1 bits (2278), Expect = 2.1e-252
Identity = 428/455 (94.07%), Postives = 446/455 (98.02%), Query Frame = 0


Query: 1   MASPSPLSFFYLLLFSSLSAIAHSNPITLPLNSFPHLSSPDPLQALTFLASSSQTRAHQI 60
           MASPSPLSFFY+LLFSSLSAI++SNPITLPLNS PHLSS DPLQALTFLAS+S+ RAH+I
Sbjct: 1   MASPSPLSFFYILLFSSLSAISNSNPITLPLNSSPHLSSSDPLQALTFLASASKNRAHRI 60

Query: 61  KTPKSNSVFKSPLSPHSYGAYSTPLSFGTPQQTLHLIFDTGSSLVWFPCTSRYLCSECSF 120
           KTPKSNSV KSPLSPHSYGAYSTPLSFGTPQQTLHLIFDTGSSLVWFPCTSRYLC+ECSF
Sbjct: 61  KTPKSNSVSKSPLSPHSYGAYSTPLSFGTPQQTLHLIFDTGSSLVWFPCTSRYLCTECSF 120

Query: 121 PKIDPTGIPRFVPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAYVV 180
           PKIDPTGIPRFVPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAYVV
Sbjct: 121 PKIDPTGIPRFVPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAYVV 180

Query: 181 QYGSGSTAGLLLSETLDFPDKKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLKKF 240
           QYGSGSTAGLLLSETLDFP+KKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLKKF
Sbjct: 181 QYGSGSTAGLLLSETLDFPNKKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLKKF 240

Query: 241 AYCLASRKFDDSPHSGQLILDSTGVKSSGLTYTPFRQNPSVSNNAYKEYYYLNIRKIIVG 300
           AYCLASRKFDDS HSGQLILDS+GVK+SGLTYT FRQNPSVSN+AYKEYYYLNIRKIIVG
Sbjct: 241 AYCLASRKFDDSAHSGQLILDSSGVKTSGLTYTSFRQNPSVSNHAYKEYYYLNIRKIIVG 300

Query: 301 NQAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVLEVVAREFEKQLANWTRATDVETL 360
           NQAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVL+VVA+EFEKQLAN TRATDVETL
Sbjct: 301 NQAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVLDVVAQEFEKQLANRTRATDVETL 360

Query: 361 TGLRPCFDISKEKSVKFPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTHQMEDGG 420
           TGLRPCFD+SKEKSV+FPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTH  EDGG
Sbjct: 361 TGLRPCFDVSKEKSVEFPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTHNTEDGG 420

Query: 421 GGGPSVILGAFQQQNFYVEYDLVNQRLGFRQQTCS 456
           GGGPSVILGAFQQQNFYVEYDLVN+RLGFR+QTC+
Sbjct: 421 GGGPSVILGAFQQQNFYVEYDLVNERLGFRKQTCT 455

[Close]

BLAST of CSPI03G35440 vs. NCBI nr
Match: XP_038905730.1 (probable aspartyl protease At4g16563 [Benincasa hispida])

HSP 1 Score: 870.5 bits (2248), Expect = 6.2e-249
Identity = 420/455 (92.31%), Postives = 441/455 (96.92%), Query Frame = 0


Query: 1   MASPSPLSFFYLLLFSSLSAIAHSNPITLPLNSFPHLSSPDPLQALTFLASSSQTRAHQI 60
           MA PS LSFFY+LLFSS+SAIA++NPITLPLN+FPHLSS DPLQ LTFLAS+SQ RAHQI
Sbjct: 1   MAPPSSLSFFYILLFSSVSAIANTNPITLPLNAFPHLSSSDPLQTLTFLASASQNRAHQI 60

Query: 61  KTPKSNSVFKSPLSPHSYGAYSTPLSFGTPQQTLHLIFDTGSSLVWFPCTSRYLCSECSF 120
           KTPKSNSV KSPL PHSYGAYSTPLSFGTPQQTLHLIFDTGSSLVWFPCTSRYLCSECSF
Sbjct: 61  KTPKSNSVSKSPLFPHSYGAYSTPLSFGTPQQTLHLIFDTGSSLVWFPCTSRYLCSECSF 120

Query: 121 PKIDPTGIPRFVPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAYVV 180
           PKIDPTGIPRFVPKLSSSSKLVGCQNPKCAWIFGP+VKSQCRSCNPKTENCTQTCPAYVV
Sbjct: 121 PKIDPTGIPRFVPKLSSSSKLVGCQNPKCAWIFGPEVKSQCRSCNPKTENCTQTCPAYVV 180

Query: 181 QYGSGSTAGLLLSETLDFPDKKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLKKF 240
           QYGSGSTAGLLLSETLDFPDKKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLKKF
Sbjct: 181 QYGSGSTAGLLLSETLDFPDKKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLKKF 240

Query: 241 AYCLASRKFDDSPHSGQLILDSTGVKSSGLTYTPFRQNPSVSNNAYKEYYYLNIRKIIVG 300
           AYCLASRKFDDSPHSG+LILDSTGVK+SGL+YTPFRQNPSVSN+AYKEYYYLNIRKI VG
Sbjct: 241 AYCLASRKFDDSPHSGELILDSTGVKTSGLSYTPFRQNPSVSNHAYKEYYYLNIRKIFVG 300

Query: 301 NQAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVLEVVAREFEKQLANWTRATDVETL 360
           NQAVKVPYK+LVPGPDGNGGSIIDSGSTFTFMDKPV E VA+EFEKQLAN TRATDVE+L
Sbjct: 301 NQAVKVPYKFLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEFEKQLANRTRATDVESL 360

Query: 361 TGLRPCFDISKEKSVKFPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTHQMEDGG 420
           TGLRPCFDISK+KSV+FPELIFQFKGGAKWALPL+NYFALVSSSGVACLTVVTH+ E GG
Sbjct: 361 TGLRPCFDISKDKSVEFPELIFQFKGGAKWALPLSNYFALVSSSGVACLTVVTHKTEAGG 420

Query: 421 GGGPSVILGAFQQQNFYVEYDLVNQRLGFRQQTCS 456
           GGGPSVI GAFQQQNFYVEYDLVN++LGFRQQTC+
Sbjct: 421 GGGPSVIFGAFQQQNFYVEYDLVNEKLGFRQQTCT 455

[Close]

BLAST of CSPI03G35440 vs. NCBI nr
Match: XP_023528159.1 (probable aspartyl protease At4g16563 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 821.6 bits (2121), Expect = 3.3e-234
Identity = 401/457 (87.75%), Postives = 424/457 (92.78%), Query Frame = 0


Query: 1   MASPSPLSFFYLLLFSSLSAIAHSNPITLPLNSFPHLSSPDPLQALTFLASSSQTRAHQI 60
           MA P  L FFY+LL SS+SAIA +NPITLPL+SFPH SS DPLQ L FLAS+SQ RAHQI
Sbjct: 1   MAPPPLLCFFYILLLSSVSAIADTNPITLPLSSFPHHSSSDPLQTLNFLASASQNRAHQI 60

Query: 61  KTP--KSNSVFKSPLSPHSYGAYSTPLSFGTPQQTLHLIFDTGSSLVWFPCTSRYLCSEC 120
           K P  KSNSV KSPLSPHSYGAYSTPLSFGTP QTLHLIFDTGSSLVW PCTS+YLCSEC
Sbjct: 61  KAPKSKSNSVSKSPLSPHSYGAYSTPLSFGTPSQTLHLIFDTGSSLVWLPCTSKYLCSEC 120

Query: 121 SFPKIDPTGIPRFVPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY 180
           SFPKIDP GIPRF+PKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY
Sbjct: 121 SFPKIDPAGIPRFIPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY 180

Query: 181 VVQYGSGSTAGLLLSETLDFPDKKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240
           VVQYGSGSTAGLLLSETLDF +KKI NFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK
Sbjct: 181 VVQYGSGSTAGLLLSETLDFANKKITNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240

Query: 241 KFAYCLASRKFDDSPHSGQLILDSTGVKSSGLTYTPFRQNPSVSNNAYKEYYYLNIRKII 300
           KFAYCLASRKFDDSPH+G+LILDS+G K+SGLTYTPFRQNPSVSN+AYKEYYYL IRKI 
Sbjct: 241 KFAYCLASRKFDDSPHAGELILDSSGAKTSGLTYTPFRQNPSVSNHAYKEYYYLTIRKIF 300

Query: 301 VGNQAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVLEVVAREFEKQLANWTRATDVE 360
           VGN+AVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPV E VA+E EKQLAN TRATDVE
Sbjct: 301 VGNKAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEIEKQLANRTRATDVE 360

Query: 361 TLTGLRPCFDISKEKSVKFPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTHQMED 420
           +LTGLRPCFDISK+KSV+FPEL FQ KGGAKWALPL+NYFALVSSSGVACLTVVTH+  D
Sbjct: 361 SLTGLRPCFDISKDKSVEFPELTFQLKGGAKWALPLSNYFALVSSSGVACLTVVTHKAAD 420

Query: 421 GGGGGPSVILGAFQQQNFYVEYDLVNQRLGFRQQTCS 456
             GGGPS+ILGAFQQQNFYVEYDLVNQ++GFRQQTCS
Sbjct: 421 -SGGGPSIILGAFQQQNFYVEYDLVNQKIGFRQQTCS 456

[Close]

BLAST of CSPI03G35440 vs. NCBI nr
Match: XP_022982947.1 (probable aspartyl protease At4g16563 [Cucurbita maxima])

HSP 1 Score: 820.8 bits (2119), Expect = 5.6e-234
Identity = 398/457 (87.09%), Postives = 423/457 (92.56%), Query Frame = 0


Query: 1   MASPSPLSFFYLLLFSSLSAIAHSNPITLPLNSFPHLSSPDPLQALTFLASSSQTRAHQI 60
           MA P PL FFY+LL SS+SAIA +NPIT+PL+SFPH SS DPLQ L FLAS+SQ RAHQI
Sbjct: 1   MAPPPPLCFFYILLVSSVSAIADTNPITIPLSSFPHHSSSDPLQTLNFLASASQNRAHQI 60

Query: 61  KTPK--SNSVFKSPLSPHSYGAYSTPLSFGTPQQTLHLIFDTGSSLVWFPCTSRYLCSEC 120
           K PK  SNSV KSPLSPHSYGAYSTPLSFGTP QTLHLIFDTGSSLVW PCTS+YLCSEC
Sbjct: 61  KAPKSESNSVSKSPLSPHSYGAYSTPLSFGTPPQTLHLIFDTGSSLVWLPCTSKYLCSEC 120

Query: 121 SFPKIDPTGIPRFVPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY 180
           SFPKIDP GIPRF+PKLSS+SKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY
Sbjct: 121 SFPKIDPAGIPRFIPKLSSTSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY 180

Query: 181 VVQYGSGSTAGLLLSETLDFPDKKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240
           VVQYGSGSTAGLLLSETLDFPDKK  NFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK
Sbjct: 181 VVQYGSGSTAGLLLSETLDFPDKKFTNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240

Query: 241 KFAYCLASRKFDDSPHSGQLILDSTGVKSSGLTYTPFRQNPSVSNNAYKEYYYLNIRKII 300
           KFAYCLASRKFDDSPH+G+LILDS+G K+SGL+YTPFRQNPSVSN+AYKEYYYL IRKI 
Sbjct: 241 KFAYCLASRKFDDSPHAGELILDSSGAKTSGLSYTPFRQNPSVSNHAYKEYYYLTIRKIF 300

Query: 301 VGNQAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVLEVVAREFEKQLANWTRATDVE 360
           VG +AVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPV E VA+E EKQLAN TRATDVE
Sbjct: 301 VGKKAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEIEKQLANRTRATDVE 360

Query: 361 TLTGLRPCFDISKEKSVKFPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTHQMED 420
           +LTGLRPCFDISK+KSV+FPEL FQ KGGAKW LPL+NYFALVSSSGVACLTVVTH+  D
Sbjct: 361 SLTGLRPCFDISKDKSVEFPELTFQLKGGAKWGLPLSNYFALVSSSGVACLTVVTHKTAD 420

Query: 421 GGGGGPSVILGAFQQQNFYVEYDLVNQRLGFRQQTCS 456
             GGGPS+ILGAFQQQNFYVEYDLVNQ++GFRQQTCS
Sbjct: 421 -SGGGPSIILGAFQQQNFYVEYDLVNQKIGFRQQTCS 456

[Close]

BLAST of CSPI03G35440 vs. TAIR 10
Match: AT3G52500.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 538.1 bits (1385), Expect = 6.8e-153
Identity = 269/474 (56.75%), Postives = 341/474 (71.94%), Query Frame = 0


Query: 5   SPLSFFYLLLFSSLSAIAHSNPITLPLNSFPH--LSSPDPLQALTFLASSSQTRAHQIK- 64
           S + FF+L+  S +SA      + LPL+ F H   S  DP  +L  LA SS  RAH++K 
Sbjct: 3   SSIFFFFLIFLSVVSA------VKLPLSPFSHSDQSPKDPYLSLRRLAESSIARAHKLKH 62

Query: 65  --------------TPKSNSVFKSPLSPHSYGAYSTPLSFGTPQQTLHLIFDTGSSLVWF 124
                         T  S +V KSPLS  SYG YS  LSFGTP QT+  +FDTGSSLVW 
Sbjct: 63  GTSIKPDEDALSSTTTASATVVKSPLSAKSYGGYSVSLSFGTPSQTIPFVFDTGSSLVWL 122

Query: 125 PCTSRYLCSECSFPKIDPTGIPRFVPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPK 184
           PCTSRYLCS C F  +DPT IPRF+PK SSSSK++GCQ+PKC +++GP+V  QCR C+P 
Sbjct: 123 PCTSRYLCSGCDFSGLDPTLIPRFIPKNSSSSKIIGCQSPKCQFLYGPNV--QCRGCDPN 182

Query: 185 TENCTQTCPAYVVQYGSGSTAGLLLSETLDFPDKKIPNFVVGCSFLSIHQPSGIAGFGRG 244
           T NCT  CP Y++QYG GSTAG+L++E LDFPD  +P+FVVGCS +S  QP+GIAGFGRG
Sbjct: 183 TRNCTVGCPPYILQYGLGSTAGVLITEKLDFPDLTVPDFVVGCSIISTRQPAGIAGFGRG 242

Query: 245 SESLPSQMGLKKFAYCLASRKFDDSPHSGQLILD-----STGVKSSGLTYTPFRQNPSVS 304
             SLPSQM LK+F++CL SR+FDD+  +  L LD     ++G K+ GLTYTPFR+NP+VS
Sbjct: 243 PVSLPSQMNLKRFSHCLVSRRFDDTNVTTDLDLDTGSGHNSGSKTPGLTYTPFRKNPNVS 302

Query: 305 NNAYKEYYYLNIRKIIVGNQAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVLEVVAR 364
           N A+ EYYYLN+R+I VG + VK+PYKYL PG +G+GGSI+DSGSTFTFM++PV E+VA 
Sbjct: 303 NKAFLEYYYLNLRRIYVGRKHVKIPYKYLAPGTNGDGGSIVDSGSTFTFMERPVFELVAE 362

Query: 365 EFEKQLANWTRATDVETLTGLRPCFDISKEKSVKFPELIFQFKGGAKWALPLNNYFALVS 424
           EF  Q++N+TR  D+E  TGL PCF+IS +  V  PELIF+FKGGAK  LPL+NYF  V 
Sbjct: 363 EFASQMSNYTREKDLEKETGLGPCFNISGKGDVTVPELIFEFKGGAKLELPLSNYFTFVG 422

Query: 425 SSGVACLTVVTHQ-MEDGGGGGPSVILGAFQQQNFYVEYDLVNQRLGFRQQTCS 456
           ++   CLTVV+ + +   GG GP++ILG+FQQQN+ VEYDL N R GF ++ CS
Sbjct: 423 NTDTVCLTVVSDKTVNPSGGTGPAIILGSFQQQNYLVEYDLENDRFGFAKKKCS 468

[Close]

BLAST of CSPI03G35440 vs. TAIR 10
Match: AT4G16563.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 194.1 bits (492), Expect = 2.4e-49
Identity = 149/501 (29.74%), Postives = 224/501 (44.71%), Query Frame = 0


Query: 7   LSFFYLLLFSSLSA-----IAHSNPITLPLNSFPHLSSPDPLQALTFLASSSQTRAHQIK 66
           L +++    SSLS      ++HS      L++  H SSP  L   +   SS++ R H  K
Sbjct: 14  LQYYFHFSVSSLSTPLLLHLSHS------LSTSKHSSSPLHLLKSSSSRSSARFRRHHHK 73

Query: 67  TPKSNSVFKSPLSPHSYGAYSTPLSFGTPQQTLHLIFDTGSSLVWFPCTSRYLCSECSFP 126
             +       P+S  S   Y   LS G+    + L  DTGS LVWFPC   + C  C   
Sbjct: 74  --QQQQQLSLPIS--SGSDYLISLSVGSSSSAVSLYLDTGSDLVWFPCRP-FTCILCESK 133

Query: 127 KIDPTGIPRFVPKLSSSSKLVGCQNPKCAWIFGPDVKSQ-CRSCN-----PKTENCTQT- 186
            + P+        LSSS+  V C +P C+        S  C   N      +T +C  + 
Sbjct: 134 PLPPSP----PSSLSSSATTVSCSSPSCSAAHSSLPSSDLCAISNCPLDFIETGDCNTSS 193

Query: 187 --CPAYVVQYGSGSTAGLLLSETLDFPDKKIPNFVVGCSFLSIHQPSGIAGFGRGSESLP 246
             CP +   YG GS    L S++L  P   + NF  GC+  ++ +P G+AGFGRG  SLP
Sbjct: 194 YPCPPFYYAYGDGSLVAKLYSDSLSLPSVSVSNFTFGCAHTTLAEPIGVAGFGRGRLSLP 253

Query: 247 SQMGL------KKFAYCLASRKFDDS--PHSGQLIL--------------------DSTG 306
           +Q+ +        F+YCL S  FD         LIL                    D   
Sbjct: 254 AQLAVHSPHLGNSFSYCLVSHSFDSDRVRRPSPLILGRFVDKKEKRVGTTDDHDDGDDEK 313

Query: 307 VKSSGLTYTPFRQNPSVSNNAYKEYYYLNIRKIIVGNQAVKVPYKYLVPGPDGNGGSIID 366
            K +   +T   +NP      +  +Y ++++ I +G + +  P        +G GG ++D
Sbjct: 314 KKKNEFVFTEMLENPK-----HPYFYSVSLQGISIGKRNIPAPAMLRRIDKNGGGGVVVD 373

Query: 367 SGSTFTFMDKPVLEVVAREFEKQLAN-WTRATDVETLTGLRPCFDISKEKSVKFPELIFQ 426
           SG+TFT +       V  EF+ ++     RA  VE  +G+ PC+ ++  ++VK P L+  
Sbjct: 374 SGTTFTMLPAKFYNSVVEEFDSRVGRVHERADRVEPSSGMSPCYYLN--QTVKVPALVLH 433

Query: 427 FKGG-AKWALPLNNYFALVSSSG--------VACLTVVTHQMEDGGGGGPSVILGAFQQQ 456
           F G  +   LP  NYF      G        + CL ++    E    GG   ILG +QQQ
Sbjct: 434 FAGNRSSVTLPRRNYFYEFMDGGDGKEEKRKIGCLMLMNGGDESELRGGTGAILGNYQQQ 492

[Close]

BLAST of CSPI03G35440 vs. TAIR 10
Match: AT5G45120.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 189.5 bits (480), Expect = 5.9e-48
Identity = 155/490 (31.63%), Postives = 226/490 (46.12%), Query Frame = 0


Query: 1   MASPSPLSFFYLLLFSSLS------AIAHSNPITLPLNSFPHLSSPDPLQALTFLASSSQ 60
           M + + + F +LL+   L+      A  H NP +   +SF  L+      +L    S +Q
Sbjct: 1   METQTHVLFLFLLITLLLNTTNKTQARQHKNP-SSSSSSFLVLTLTKSSVSLPTPKSQTQ 60

Query: 61  TRAHQIKTPKSN-SVFKSPLSPHSYGAYSTPLSFGTPQQTLHLIFDTGSSLVWFPCTS-R 120
            R   IK P S+  V   PL     G Y   L+ GTP Q + +  DTGS L W PC +  
Sbjct: 61  ER---IKKPLSSVDVVMEPLREVRDG-YLITLNIGTPPQAVQVYLDTGSDLTWVPCGNLS 120

Query: 121 YLCSECSFPKIDPTGIPR-FVPKLSSSSKLVGCQNPKCAWI------FGPDVKSQCRSCN 180
           + C EC   K +    P  F P  SS+S    C +  C  I      F P   + C    
Sbjct: 121 FDCIECYDLKNNDLKSPSVFSPLHSSTSFRDSCASSFCVEIHSSDNPFDPCAVAGCSVSM 180

Query: 181 PKTENCTQTCPAYVVQYGSGS-TAGLLLSETLDFPDKKIPNFVVGCSFLSIHQPSGIAGF 240
                C + CP++   YG G   +G+L  + L    + +P F  GC   +  +P GIAGF
Sbjct: 181 LLKSTCVRPCPSFAYTYGEGGLISGILTRDILKARTRDVPRFSFGCVTSTYREPIGIAGF 240

Query: 241 GRGSESLPSQMGL--KKFAYCLASRKFDDSPH-SGQLILDSTGVK---SSGLTYTPFRQN 300
           GRG  SLPSQ+G   K F++C    KF ++P+ S  LIL ++ +    +  L +TP    
Sbjct: 241 GRGLLSLPSQLGFLEKGFSHCFLPFKFVNNPNISSPLILGASALSINLTDSLQFTPMLNT 300

Query: 301 PSVSNNAYKEYYYLNIRKIIVGNQ--AVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPV 360
           P   N+     YY+ +  I +G      +VP         GNGG ++DSG+T+T + +P 
Sbjct: 301 PMYPNS-----YYIGLESITIGTNITPTQVPLTLRQFDSQGNGGMLVDSGTTYTHLPEPF 360

Query: 361 LEVVAREFEKQLANWTRATDVETLTGLRPCFDI--------SKEKSVK--FPELIFQFKG 420
              +    +  +  + RAT+ E+ TG   C+ +        S E  V   FP + F F  
Sbjct: 361 YSQLLTTLQSTI-TYPRATETESRTGFDLCYKVPCPNNNLTSLENDVMMIFPSITFHFLN 420

Query: 421 GAKWALPLNNYFALVS--SSGVACLTVVTHQMEDGGGGGPSVILGAFQQQNFYVEYDLVN 455
            A   LP  N F  +S  S G     ++   MED G  GP+ + G+FQQQN  V YDL  
Sbjct: 421 NATLLLPQGNSFYAMSAPSDGSVVQCLLFQNMED-GDYGPAGVFGSFQQQNVKVVYDLEK 478

[Close]

BLAST of CSPI03G35440 vs. TAIR 10
Match: AT2G42980.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 174.1 bits (440), Expect = 2.6e-43
Identity = 127/398 (31.91%), Postives = 183/398 (45.98%), Query Frame = 0


Query: 79  GAYSTPLSFGTPQQTLHLIFDTGSSLVWFPCTSRYLCSECSFPKIDPTGIPRFVPKLSSS 138
           G Y   +  GTP +   LI DTGS L W  C   Y C   +    D        PK S+S
Sbjct: 158 GEYFMDVLVGTPPKHFSLILDTGSDLNWLQCLPCYDCFHQNGMFYD--------PKTSAS 217

Query: 139 SKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAYVVQYGSGS-TAGLLLSETLD 198
            K + C +P+C+ I  PD   QC S N       Q+CP Y   YG  S T G    ET  
Sbjct: 218 FKNITCNDPRCSLISSPDPPVQCESDN-------QSCP-YFYWYGDRSNTTGDFAVETFT 277

Query: 199 F---------PDKKIPNFVVGCSFLS---IHQPSGIAGFGRGSESLPSQMGL---KKFAY 258
                      + K+ N + GC   +       SG+ G GRG  S  SQ+       F+Y
Sbjct: 278 VNLTTTEGGSSEYKVGNMMFGCGHWNRGLFSGASGLLGLGRGPLSFSSQLQSLYGHSFSY 337

Query: 259 CLASRKFDDSPHSGQLIL--DSTGVKSSGLTYTPFRQNPSVSNNAYKEYYYLNIRKIIVG 318
           CL  R   ++  S +LI   D   +  + L +T F        N+ + +YY+ I+ I+VG
Sbjct: 338 CLVDRN-SNTNVSSKLIFGEDKDLLNHTNLNFTSFVNG---KENSVETFYYIQIKSILVG 397

Query: 319 NQAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVLEVVAREF-EKQLANWTRATDVET 378
            +A+ +P +      DG+GG+IIDSG+T ++  +P  E++  +F EK   N+    D   
Sbjct: 398 GKALDIPEETWNISSDGDGGTIIDSGTTLSYFAEPAYEIIKNKFAEKMKENYPIFRDFPV 457

Query: 379 LTGLRPCFDIS--KEKSVKFPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTHQME 438
           L    PCF++S  +E ++  PEL   F  G  W  P  N F  +S   + CL ++     
Sbjct: 458 LD---PCFNVSGIEENNIHLPELGIAFVDGTVWNFPAENSFIWLSED-LVCLAIL----- 517

Query: 439 DGGGGGPSVILGAFQQQNFYVEYDLVNQRLGFRQQTCS 456
            G       I+G +QQQNF++ YD    RLGF    C+
Sbjct: 518 -GTPKSTFSIIGNYQQQNFHILYDTKRSRLGFTPTKCA 525

[Close]

BLAST of CSPI03G35440 vs. TAIR 10
Match: AT2G03200.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 162.9 bits (411), Expect = 5.9e-40
Identity = 158/494 (31.98%), Postives = 216/494 (43.72%), Query Frame = 0


Query: 2   ASPSPLSFFYLLLFSSLSAIAHSN----PITLPLN--------SFPHLSSPDPLQALTFL 61
           +S S L  F+L+LFS L +++ S       TLP N        S  H+ S   L  +  +
Sbjct: 5   SSSSLLFPFFLILFSCLISVSSSRRSLIDRTLPKNLPRSGFRLSLRHVDSGKNLTKIQKI 64

Query: 62  ASSSQTRAHQI------------KTPKSNSVFKSPLSPHSYGAYSTPLSFGTPQQTLHLI 121
                   H++              P   +  K+P    S G +   LS G P      I
Sbjct: 65  QRGINRGFHRLNRLGAVAVLAVASKPDDTNNIKAPTHGGS-GEFLMELSIGNPAVKYSAI 124

Query: 122 FDTGSSLVWFPCTSRYLCSECSFPKIDPTGIPRFVPKLSSSSKLVGCQNPKCAWIFGPDV 181
            DTGS L+W  C     C+EC      PT  P F P+ SSS   VGC +  C  +     
Sbjct: 125 VDTGSDLIWTQCKP---CTECF---DQPT--PIFDPEKSSSYSKVGCSSGLCNAL----- 184

Query: 182 KSQCRSCNPKTENCTQTCPAYVVQYGS-GSTAGLLLSETLDFPDK-KIPNFVVGCSFLS- 241
                +CN   + C      Y+  YG   ST GLL +ET  F D+  I     GC   + 
Sbjct: 185 --PRSNCNEDKDAC-----EYLYTYGDYSSTRGLLATETFTFEDENSISGIGFGCGVENE 244

Query: 242 ---IHQPSGIAGFGRGSESLPSQMGLKKFAYCLASRKFDDSPHSGQL--------ILDST 301
                Q SG+ G GRG  SL SQ+   KF+YCL S   +DS  S  L        I++ T
Sbjct: 245 GDGFSQGSGLVGLGRGPLSLISQLKETKFSYCLTS--IEDSEASSSLFIGSLASGIVNKT 304

Query: 302 GVKSSG-LTYT-PFRQNPSVSNNAYKEYYYLNIRKIIVGNQAVKVPYKYLVPGPDGNGGS 361
           G    G +T T    +NP         +YYL ++ I VG + + V         DG GG 
Sbjct: 305 GASLDGEVTKTMSLLRNPD-----QPSFYYLELQGITVGAKRLSVEKSTFELAEDGTGGM 364

Query: 362 IIDSGSTFTFMDKPVLEVVAREFEKQLANWTRATDVETLTGLRPCFDI-SKEKSVKFPEL 421
           IIDSG+T T++++   +V+  EF  ++   +   D    TGL  CF +    K++  P++
Sbjct: 365 IIDSGTTITYLEETAFKVLKEEFTSRM---SLPVDDSGSTGLDLCFKLPDAAKNIAVPKM 424

Query: 422 IFQFKGGAKWALPLNNYFALVSSSGVACLTVVTHQMEDGGGGGPSVILGAFQQQNFYVEY 455
           IF FK GA   LP  NY    SS+GV CL +       G   G S I G  QQQNF V +
Sbjct: 425 IFHFK-GADLELPGENYMVADSSTGVLCLAM-------GSSNGMS-IFGNVQQQNFNVLH 458

The following BLAST results are available for this feature:

BLAST of CSPI03G35440 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q940R4	3.4e-48	29.74	Probable aspartyl protease At4g16563 OS=Arabidopsis thaliana OX=3702 GN=At4g1656...	[more]
Q9LNJ3	1.2e-37	30.75	Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ...	[more]
Q766C2	9.6e-35	31.85	Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S...	[more]
Q766C3	1.4e-33	29.20	Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S...	[more]
Q6F4N5	7.6e-32	27.14	Aspartyl protease 25 OS=Oryza sativa subsp. japonica OX=39947 GN=AP25 PE=2 SV=1	[more]

BLAST of CSPI03G35440 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LBI9	4.6e-266	99.12	Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G77844...	[more]
A0A5A7TRK2	1.0e-252	94.07	Aspartic proteinase nepenthesin-2 OS=Cucumis melo var. makuwa OX=1194695 GN=E567...	[more]
A0A1S3B6B5	1.0e-252	94.07	aspartic proteinase nepenthesin-2 OS=Cucumis melo OX=3656 GN=LOC103486666 PE=3 S...	[more]
A0A6J1IXY3	2.7e-234	87.09	probable aspartyl protease At4g16563 OS=Cucurbita maxima OX=3661 GN=LOC111481640...	[more]
A0A6J1F3G5	7.9e-234	87.31	probable aspartyl protease At4g16563 OS=Cucurbita moschata OX=3662 GN=LOC1114418...	[more]

BLAST of CSPI03G35440 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004136706.1	9.5e-266	99.12	probable aspartyl protease At4g16563 [Cucumis sativus] >KGN59188.1 hypothetical ...	[more]
XP_008442902.1	2.1e-252	94.07	PREDICTED: aspartic proteinase nepenthesin-2 [Cucumis melo] >KAA0043829.1 aspart...	[more]
XP_038905730.1	6.2e-249	92.31	probable aspartyl protease At4g16563 [Benincasa hispida]	[more]
XP_023528159.1	3.3e-234	87.75	probable aspartyl protease At4g16563 [Cucurbita pepo subsp. pepo]	[more]
XP_022982947.1	5.6e-234	87.09	probable aspartyl protease At4g16563 [Cucurbita maxima]	[more]

BLAST of CSPI03G35440 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G52500.1	6.8e-153	56.75	Eukaryotic aspartyl protease family protein	[more]
AT4G16563.1	2.4e-49	29.74	Eukaryotic aspartyl protease family protein	[more]
AT5G45120.1	5.9e-48	31.63	Eukaryotic aspartyl protease family protein	[more]
AT2G42980.1	2.6e-43	31.91	Eukaryotic aspartyl protease family protein	[more]
AT2G03200.1	5.9e-40	31.98	Eukaryotic aspartyl protease family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001461	Aspartic peptidase A1 family	PRINTS	PR00792	PEPSIN	coord: 87..107 score: 50.56 coord: 321..332 score: 29.09 coord: 426..441 score: 30.89
IPR032799	Xylanase inhibitor, C-terminal	PFAM	PF14541	TAXi_C	coord: 290..450 e-value: 2.3E-32 score: 112.0
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 64..259 e-value: 4.1E-34 score: 120.2
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 260..455 e-value: 4.2E-50 score: 172.0
IPR021109	Aspartic peptidase domain superfamily	SUPERFAMILY	50630	Acid proteases	coord: 75..454
IPR032861	Xylanase inhibitor, N-terminal	PFAM	PF14543	TAXi_N	coord: 81..258 e-value: 1.9E-29 score: 103.1
None	No IPR available	PANTHER	PTHR47967:SF36	BNACNNG47670D PROTEIN	coord: 8..454
None	No IPR available	PANTHER	PTHR47967	OS07G0603500 PROTEIN-RELATED	coord: 8..454
IPR001969	Aspartic peptidase, active site	PROSITE	PS00141	ASP_PROTEASE	coord: 96..107
IPR033121	Peptidase family A1 domain	PROSITE	PS51767	PEPTIDASE_A1	coord: 81..450 score: 33.401329
IPR034161	Pepsin-like domain, plant	CDD	cd05476	pepsin_A_like_plant	coord: 80..454 e-value: 3.67328E-80 score: 247.561

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G35440.1	CSPI03G35440.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0004190	aspartic-type endopeptidase activity