CSPI03G33330 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI03G33330
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionPhosphoglycerate kinase
LocationChr3: 29439938 .. 29445834 (-)
RNA-Seq ExpressionCSPI03G33330
SyntenyCSPI03G33330
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAAACGCACCCTCTACATGCCAACGAAACGTGGGGGAGGAGAGAGAAAAAGAAAAGTGAAGTCCGGAGGGAGATAACACATGAAGGAAAGAAGGAAGACAATGGATGACCGGAAAATTGGTCGGAGATGCCTCTAAGAAAGGTTTCAAATCGGTAAGCTCATTTCTTTTTAAAAGAAAACATGCAAATGGAGTATTTATATCATGGGTCTCATTTTCCACGATATAAAGTTTCAATTTTAGGCGTATTTTATCTAGTTTTCAGGAGAAACTTTGCCGGAAACTTTTATGAATTATCATCTAGTAAGTTTCATTCTAAAAACTATTACAATTTAAAACCATACTCGTTGCTAGCCACTTAATTTGATAAACATTTTTAGTGTTCAACTATAAGTTCATAGTTTAGGTGGAATCGATTATCGATATGCCACGTACTCTGGGATGGAGTCTCCCAATGCTCTCAGAGACTTTGAAGAGCTGAAATACCACCAGAATTACTGATTTGAAAATCAGAAGAGTGTTGGAAAGCATAACTAATCAAAGACGTAAACAGTAAAAAAGAGGGTTGAATTGAAAATGGATGGAATGAGTTAAGTTTTTATATTACAAATTGGATCAAAACGCTTTTTACAAACGTGAGTTTTGGATTGTGTTACGTTACAAATCTATCTCATTACGGAAGACTAAACACCTCCTTACGGTCTTACCTATTTGGTTCATTCTCAAATTTGAAAGTTCTCTACCTCATGTTGTTTTATGATTGGTTTGTTGCAGTCTAGTGCAGAATTCTCGTTTCTCGAATAAGGAAAAAGGAGATGGGCCAGCTTTTAAATCCACCTCAAGGAATTATTTTCGTTATTAAAGCAGTTGCTCAAGATCCAGCCAAGTTATATCGTCAAAATTTGTCTCCATATTTCTCCAAATATGACAGATATCATGCTGTCTTAATGTCAACCCCAGGTATAAAGTTAATTTTTCTCTATATATTAAACTTTGAGTTTGCGCCTTAATCTTATACAAGTATGTCTTGTTTGAAATGCTTCTCAGCACGAAGTTGGGGGGCAGTCTATTGTAAGAACTTGGTGAACTCGGAGGTATTTAAAAGGCTCAGTCTTTGCCATCCAATTTATTTAATGATGGAAAGGGAACTTTAGGATTTTATTTCTCTGTGTATAACTTAAGTTTTCAGTATCCTACTTTCTTTGTCTGAGTTTTTTAAACTTGATTTGATTTCTTTGTTTCTTTTTTTTTGCCTTAAGTGAAGTTTGCCATTTAAAAGTCCAACACATGTTCTATCATTCCAAGTATTGTGTATAACTGGCTTGTGGTCTTAATAAGAATTTGTGGATCCTGTAGGACCATGCTTCCAGTTGGTGGAATTTAGAGGCTGTTCCACATGTACAAACTCTTGGAGAGTTTCCCAGGGAAGAACTTGCCGGAAAAGTTGTAATGGTCAGGTTTGATTCAACATTACTGCTACTGGAAGAAATGGACCTAAAGACTAACTCGATGAAGAATGCAGTGTTCACCATTAAATACTTGCAGAAATCTGGAGCAAAAGTAATACTTGCTAGCAACTGGAATACAAACTCTGCTGCAAGATATCTGGATATAAAGTCTGTCGTGGGTACACATTCTAGGATGTTTTCTGGTTTCGATATAGTACACTGTTGTTTCATTTTAAGAATGGAGATAATTCATTATGATTTTGTTATTGTTATTATTTTATTGGAGATAATTCATTATGTTGATTCTACATGAATATGTCGAAGTAGGATTGAAGTAAATAGACAACCATAATGCATGAAAGTTAAACCTTAACAGAGCTCCACTTTTTAGACTTCAGATGGTTTTGGTTTGTTTTGATTTTACTTTATGCTTAATAATTGATGGCTTGCAATTACAGATTATTTGTCCTCTGTTCTTCAACTCAAAGTTTGCCCGGTAAAGTGCAGTTCGTATAGTATGATGTTAACCGAAGAAGGCTTTGAGAAAGCTGATATTCTCCTTCTCGAGAATCTTTCCGTGTTCAAAGAAGAAGTTTCGAATTGCTCAAAATTTTCCAAGATGCTATCTTTAGGTGTTGATATTTTTGTTAATGACTCCTTCTCCCAATCTCATAGAATTCTTGCATCCACAGTTGGGATTACTCGTTTTTGCAGTACCTGCCTAGCTGGCTTTAACTTTCAAGAAGGTTTATGCCAGTTAAAAAAAGCTGCACAAACCAAGAGGCAACCTTATGTTGCAATTGTATATTCTCCACTACTTGAATAGATATGGATTTTCCCCTCATCAGCCATAAAACGATTCTATGTAATGTATTATGAACTTTTTGATGCAGATTGGAGGAGGCAACCTCTTAAACAAATTGGCTGCCTTGCACGTTTTAGCTTCAACATGCTCTGTGTTGGTCTTTGTTGGAATGATGTCATTTCAGATCATGCAAGCCTTGGGGCTGTCTGTTTCTTACAGATTGATGAATCATGGTGTATGTAAGGAAGCTGCAGAACTAATCCAGTTTTCACTTGATAAAAGGGTACGAATTGTATACCCCAAGGACTTCTGGTGTGCGAACGTTGACACTTCAAAGAAAATGGAAATATTTGCTTCTCATGATATTCCAGATGGTAAGTTCTCATTTTCCCCAACTCAAATGAATATTTATCCTTTTTAAGGTTTTACACTTCGTATTTAAAAACTTGTTATTTTGTTATATTAACTTCTTCCGCTTCTAGGTTGGCTACCAGTTGATCTTGGTCCTACATCACTGGACGAAATAAATGCCCTGCTTATGAATAGCAAGGTAGAATTCAAATTTTCCTTAAGTTACAAAAGTTGCTTCTGCTTGATCTAAAATCCCATGTATCTGCCATTGCCTGGTAAGTGGTAGCTATGAACCATAATTTTATGCTCAGCACCTTGGGTTGTCCAATAGTTTATGATTTAGCTCCTCGGCTTGTTCAATAGATCTATGATTTAGCACCTCGGGTTGTCCAACTTCTCTTTTATTTAGAAAACTGCTGTAATTTTTGGCATGCGAAGAAAAACTAGGATAGGTCAAATCATTCCAATTTCTGCTTTATTTGAATATCAATGGAACACTATCTACTATCTACTTTCATTCAAATTGTAGGGATTTGATCATTTTACTGCTCCTATGACTTTTTTATTCCTTTGATGTATATGCCTGTGCAATCAAACTTGAAATTGTGGGGATTCAAATCTTTGGGTAAGGGTTGGAGAAGAAGAATTGGCATTTTTTGAATGATTGAATTATGAAAGCATTGATCTCCTGAGATAATATTACAGATCCTAAGATTTAGCACTTATGGTCATGGACTTACAGTTGGAAGAGGTTCAATAATGCATCATTCTGCAATCATTTTTCTAAAGCACCAACTATTACTTATCTGTTATTTACTCAAGTGAGGATCGGTCTAACACTTTGAGCACTATGTTGCAGAAAATTATATGGATTGGACCTGTGAAATTCAGTGATTCAAGCCAAAGTGCCCGTGGAGCTTCTAGATTAGCAAGAAAGCTATATGATCTATCCCAAAGGGATTGTGATATAACTGTTGTTGGAACCACTGCATGCAAAGCAATTATGCAGGAATCGAGTACCCTATCTGCTTATAATGTTTTTGAGAATGCTTCAGTTGTATGGGATTTCTTTAAAGGGAAACAACTTCCAGGGGTTTTGGCCTTGGATAGAGTAAGTTTTTAGGCATTTTTGTTATATTTTCTAAAAAACAAATGATGTGTTTAAATTGTTTCAACGAAGTGCAGAACATAATGTGAACTTATAGGGGGAATGTCATAAATCGTATTCACACGTACTATTTGATGATTGATTTTTTCAATATTACACGTGCTGTCTAGTTTTGCAAGGCTACAATTTCCATTTATCATCATTTCTTGTGCAGAAAGTTCTTATTTTAGAACGGTATTACTCCATTAAAGACATCCCCACCATAGGATAGATTTTTGAAGTATGCATCTGGATGAGCAGCTAAAACTAGTTTGAAACGATTTGTTCATGCAGGCATACCCATATGAGATTAACTGGGATGCAGTGTTTTGTGATACAGCTCTGCCTTTAGTGGTTGACATTGGAAGTGGTATTATCTTCTTTGTATCATATACTTTTTTTGGGGGGGATTAAAATTCTTTCTATCCCTTTTATAGTAAAACTCTGTATCTAACAGCTAAAAGGTTGATTTTCATGCTATCTAACATTTTTGGTACAAGTGAATGGTTTTTTCTTTTGTTGGTACAACTGTACAAGTTTAAAGAAGCCAATGACGATTGCATTTAATTGTTTATTCACGAAGTTAGCTGATAAGTTGTAAGTTGTAAATGCCAGGTAATGGGATGTTTCTTCTGGAAATGGCTAGGAGGAGGAAGGATCACAATTTTCTTGGTTTAGAGATTAATGAAAAGGTCTTCTTTCTTGTCATTATCAGCATTAACCTTAACCATATACATTATCTCACACTTGTACGACCTTTTCAGCTTGTGAAACGTTGTCTACATTCTGTTCATCAATTGGAGATGAAGAATGGGTAAGGCATATCAATGAAGCATCCTCTGTCCCTGACGCTAGGTTAACATGTACCTTTGGATCGGGTTTTTTGTTTCTTTACTAAATTTTATCTCGATTTTTTCTCATGGTATCAGGCATTTTATTGCGACGAATGCAACGTCAACGTTTCGTTCTATTGTGTGTAATTATCCAGGGGAGTTGGTGCTTGTCTCTATACAGGTACACAACCCTCATGGATCATGTTGTCCCCAATATTTGGATTTTGACCCTTCCTAGCCATGATATTACTGAGAACTGATAGAATGCTGTTTGTTTTACTCTGCTATGCTACGCACTTGGAATTTTCCAGCTAATATCTCTAAATTCAGATCACTCGTAGAATTCACTACCTTCATGACTTTGAACCAGATACTGATTTCATTCTTGAAAGTTAAAGCCTTGAAGAGGCGTTGCTATTTACAAAAGCCTAACTATTAATTGCTTGATACGAGCCATTCATAGTGCTAGCTCTATCGATACTTTAAAATCTTAATTGCACACCTCTGTACCAAATATATTTACTCTCTGTATATACAGTGTCCCAACCCCGATTTCAATAAACCGGAGCACAGATGGAGAATGTTGCAAAGGTCATTAGTGGAGGCAGTGGCAGACTTGCTTGCGTCTAATGGCAAGGTACTACCATTTTCTCAGTGCATCTTCACCTTTTTTTTCTTTTCAAGTAAATGTTCATCTTGGATGTTGGACTTCTCTTATGTCATATGGTTATTACTCTTGTTACGATGTGACTGGATTCCTATTTGTTAGGTCTTTCTTCAATCTGATGTTGAAGCTGTTGCTTTAAGAATGAGGGAAGCTTTCTTACTATATGGAAAAGGGAAACTAGGTGTTTTGCAAGAACAAGTTGGGGAAGAATGGCTAAGTGAGAACCCATTTGGAGTCAGATCGGATTGGGAACGACACGTATTAGATCGTGGGGATCCTATGTTCAGGTTAATGCTCTCGAAATCAACAACGACCAAATCGTTCAACTGACCAGAAAAAATTTCCTTTGTTATGGTTATTCACCACTGAAGCTCTAAGAAGCCATGAAAGTACAACCTAACATGGTCTCCTAGTTCATATACTTTCAACCTTGTGTTTTATCTAATCGACGGAGGCCGTTATCATTAACCAAGTTTGTAGTGTAGAAGCTGATCCGTCAAAGAAACTAGTGTGATAAAAAATGTTGCCTGATTCCGAAGAGCTTGACTCAACGTTAGAGAAAAGCCTTGCCCTTTGTTGCAAAGAAAAGAAAACAAAAGACTCATTTCTCCTCTGGTTCTCAATCAATTAGATTACCTTTAATAAT

mRNA sequence

AAAAAACGCACCCTCTACATGCCAACGAAACGTGGGGGAGGAGAGAGAAAAAGAAAAGTGAAGTCCGGAGGGAGATAACACATGAAGGAAAGAAGGAAGACAATGGATGACCGGAAAATTGGTCGGAGATGCCTCTAAGAAAGGTTTCAAATCGTCTAGTGCAGAATTCTCGTTTCTCGAATAAGGAAAAAGGAGATGGGCCAGCTTTTAAATCCACCTCAAGGAATTATTTTCGTTATTAAAGCAGTTGCTCAAGATCCAGCCAAGTTATATCGTCAAAATTTGTCTCCATATTTCTCCAAATATGACAGATATCATGCTGTCTTAATGTCAACCCCAGCACGAAGTTGGGGGGCAGTCTATTGTAAGAACTTGGTGAACTCGGAGGACCATGCTTCCAGTTGGTGGAATTTAGAGGCTGTTCCACATGTACAAACTCTTGGAGAGTTTCCCAGGGAAGAACTTGCCGGAAAAGTTGTAATGGTCAGGTTTGATTCAACATTACTGCTACTGGAAGAAATGGACCTAAAGACTAACTCGATGAAGAATGCAGTGTTCACCATTAAATACTTGCAGAAATCTGGAGCAAAAGTAATACTTGCTAGCAACTGGAATACAAACTCTGCTGCAAGATATCTGGATATAAAGTCTGTCGTGGATTATTTGTCCTCTGTTCTTCAACTCAAAGTTTGCCCGGTAAAGTGCAGTTCGTATAGTATGATGTTAACCGAAGAAGGCTTTGAGAAAGCTGATATTCTCCTTCTCGAGAATCTTTCCGTGTTCAAAGAAGAAGTTTCGAATTGCTCAAAATTTTCCAAGATGCTATCTTTAGGTGTTGATATTTTTGTTAATGACTCCTTCTCCCAATCTCATAGAATTCTTGCATCCACAGTTGGGATTACTCGTTTTTGCAGTACCTGCCTAGCTGGCTTTAACTTTCAAGAAGGTTTATGCCAGTTAAAAAAAGCTGCACAAACCAAGAGGCAACCTTATGTTGCAATTATTGGAGGAGGCAACCTCTTAAACAAATTGGCTGCCTTGCACGTTTTAGCTTCAACATGCTCTGTGTTGGTCTTTGTTGGAATGATGTCATTTCAGATCATGCAAGCCTTGGGGCTGTCTGTTTCTTACAGATTGATGAATCATGGTGTATGTAAGGAAGCTGCAGAACTAATCCAGTTTTCACTTGATAAAAGGGTACGAATTGTATACCCCAAGGACTTCTGGTGTGCGAACGTTGACACTTCAAAGAAAATGGAAATATTTGCTTCTCATGATATTCCAGATGGTTGGCTACCAGTTGATCTTGGTCCTACATCACTGGACGAAATAAATGCCCTGCTTATGAATAGCAAGAAAATTATATGGATTGGACCTGTGAAATTCAGTGATTCAAGCCAAAGTGCCCGTGGAGCTTCTAGATTAGCAAGAAAGCTATATGATCTATCCCAAAGGGATTGTGATATAACTGTTGTTGGAACCACTGCATGCAAAGCAATTATGCAGGAATCGAGTACCCTATCTGCTTATAATGTTTTTGAGAATGCTTCAGTTGTATGGGATTTCTTTAAAGGGAAACAACTTCCAGGGGTTTTGGCCTTGGATAGAGCATACCCATATGAGATTAACTGGGATGCAGTGTTTTGTGATACAGCTCTGCCTTTAGTGGTTGACATTGGAAGTGGTAATGGGATGTTTCTTCTGGAAATGGCTAGGAGGAGGAAGGATCACAATTTTCTTGGTTTAGAGATTAATGAAAAGCTTGTGAAACGTTGTCTACATTCTGTTCATCAATTGGAGATGAAGAATGGGCATTTTATTGCGACGAATGCAACGTCAACGTTTCGTTCTATTGTGTGTAATTATCCAGGGGAGTTGGTGCTTGTCTCTATACAGTGTCCCAACCCCGATTTCAATAAACCGGAGCACAGATGGAGAATGTTGCAAAGGTCATTAGTGGAGGCAGTGGCAGACTTGCTTGCGTCTAATGGCAAGGTCTTTCTTCAATCTGATGTTGAAGCTGTTGCTTTAAGAATGAGGGAAGCTTTCTTACTATATGGAAAAGGGAAACTAGGTGTTTTGCAAGAACAAGTTGGGGAAGAATGGCTAAGTGAGAACCCATTTGGAGTCAGATCGGATTGGGAACGACACGTATTAGATCGTGGGGATCCTATGTTCAGGTTAATGCTCTCGAAATCAACAACGACCAAATCGTTCAACTGACCAGAAAAAATTTCCTTTGTTATGGTTATTCACCACTGAAGCTCTAAGAAGCCATGAAAGTACAACCTAACATGGTCTCCTAGTTCATATACTTTCAACCTTGTGTTTTATCTAATCGACGGAGGCCGTTATCATTAACCAAGTTTGTAGTGTAGAAGCTGATCCGTCAAAGAAACTAGTGTGATAAAAAATGTTGCCTGATTCCGAAGAGCTTGACTCAACGTTAGAGAAAAGCCTTGCCCTTTGTTGCAAAGAAAAGAAAACAAAAGACTCATTTCTCCTCTGGTTCTCAATCAATTAGATTACCTTTAATAAT

Coding sequence (CDS)

ATGGGCCAGCTTTTAAATCCACCTCAAGGAATTATTTTCGTTATTAAAGCAGTTGCTCAAGATCCAGCCAAGTTATATCGTCAAAATTTGTCTCCATATTTCTCCAAATATGACAGATATCATGCTGTCTTAATGTCAACCCCAGCACGAAGTTGGGGGGCAGTCTATTGTAAGAACTTGGTGAACTCGGAGGACCATGCTTCCAGTTGGTGGAATTTAGAGGCTGTTCCACATGTACAAACTCTTGGAGAGTTTCCCAGGGAAGAACTTGCCGGAAAAGTTGTAATGGTCAGGTTTGATTCAACATTACTGCTACTGGAAGAAATGGACCTAAAGACTAACTCGATGAAGAATGCAGTGTTCACCATTAAATACTTGCAGAAATCTGGAGCAAAAGTAATACTTGCTAGCAACTGGAATACAAACTCTGCTGCAAGATATCTGGATATAAAGTCTGTCGTGGATTATTTGTCCTCTGTTCTTCAACTCAAAGTTTGCCCGGTAAAGTGCAGTTCGTATAGTATGATGTTAACCGAAGAAGGCTTTGAGAAAGCTGATATTCTCCTTCTCGAGAATCTTTCCGTGTTCAAAGAAGAAGTTTCGAATTGCTCAAAATTTTCCAAGATGCTATCTTTAGGTGTTGATATTTTTGTTAATGACTCCTTCTCCCAATCTCATAGAATTCTTGCATCCACAGTTGGGATTACTCGTTTTTGCAGTACCTGCCTAGCTGGCTTTAACTTTCAAGAAGGTTTATGCCAGTTAAAAAAAGCTGCACAAACCAAGAGGCAACCTTATGTTGCAATTATTGGAGGAGGCAACCTCTTAAACAAATTGGCTGCCTTGCACGTTTTAGCTTCAACATGCTCTGTGTTGGTCTTTGTTGGAATGATGTCATTTCAGATCATGCAAGCCTTGGGGCTGTCTGTTTCTTACAGATTGATGAATCATGGTGTATGTAAGGAAGCTGCAGAACTAATCCAGTTTTCACTTGATAAAAGGGTACGAATTGTATACCCCAAGGACTTCTGGTGTGCGAACGTTGACACTTCAAAGAAAATGGAAATATTTGCTTCTCATGATATTCCAGATGGTTGGCTACCAGTTGATCTTGGTCCTACATCACTGGACGAAATAAATGCCCTGCTTATGAATAGCAAGAAAATTATATGGATTGGACCTGTGAAATTCAGTGATTCAAGCCAAAGTGCCCGTGGAGCTTCTAGATTAGCAAGAAAGCTATATGATCTATCCCAAAGGGATTGTGATATAACTGTTGTTGGAACCACTGCATGCAAAGCAATTATGCAGGAATCGAGTACCCTATCTGCTTATAATGTTTTTGAGAATGCTTCAGTTGTATGGGATTTCTTTAAAGGGAAACAACTTCCAGGGGTTTTGGCCTTGGATAGAGCATACCCATATGAGATTAACTGGGATGCAGTGTTTTGTGATACAGCTCTGCCTTTAGTGGTTGACATTGGAAGTGGTAATGGGATGTTTCTTCTGGAAATGGCTAGGAGGAGGAAGGATCACAATTTTCTTGGTTTAGAGATTAATGAAAAGCTTGTGAAACGTTGTCTACATTCTGTTCATCAATTGGAGATGAAGAATGGGCATTTTATTGCGACGAATGCAACGTCAACGTTTCGTTCTATTGTGTGTAATTATCCAGGGGAGTTGGTGCTTGTCTCTATACAGTGTCCCAACCCCGATTTCAATAAACCGGAGCACAGATGGAGAATGTTGCAAAGGTCATTAGTGGAGGCAGTGGCAGACTTGCTTGCGTCTAATGGCAAGGTCTTTCTTCAATCTGATGTTGAAGCTGTTGCTTTAAGAATGAGGGAAGCTTTCTTACTATATGGAAAAGGGAAACTAGGTGTTTTGCAAGAACAAGTTGGGGAAGAATGGCTAAGTGAGAACCCATTTGGAGTCAGATCGGATTGGGAACGACACGTATTAGATCGTGGGGATCCTATGTTCAGGTTAATGCTCTCGAAATCAACAACGACCAAATCGTTCAACTGA

Protein sequence

MGQLLNPPQGIIFVIKAVAQDPAKLYRQNLSPYFSKYDRYHAVLMSTPARSWGAVYCKNLVNSEDHASSWWNLEAVPHVQTLGEFPREELAGKVVMVRFDSTLLLLEEMDLKTNSMKNAVFTIKYLQKSGAKVILASNWNTNSAARYLDIKSVVDYLSSVLQLKVCPVKCSSYSMMLTEEGFEKADILLLENLSVFKEEVSNCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCSTCLAGFNFQEGLCQLKKAAQTKRQPYVAIIGGGNLLNKLAALHVLASTCSVLVFVGMMSFQIMQALGLSVSYRLMNHGVCKEAAELIQFSLDKRVRIVYPKDFWCANVDTSKKMEIFASHDIPDGWLPVDLGPTSLDEINALLMNSKKIIWIGPVKFSDSSQSARGASRLARKLYDLSQRDCDITVVGTTACKAIMQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRAYPYEINWDAVFCDTALPLVVDIGSGNGMFLLEMARRRKDHNFLGLEINEKLVKRCLHSVHQLEMKNGHFIATNATSTFRSIVCNYPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLASNGKVFLQSDVEAVALRMREAFLLYGKGKLGVLQEQVGEEWLSENPFGVRSDWERHVLDRGDPMFRLMLSKSTTTKSFN*
Homology
BLAST of CSPI03G33330 vs. ExPASy Swiss-Prot
Match: Q42961 (Phosphoglycerate kinase, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 192.6 bits (488), Expect = 1.5e-47
Identity = 124/397 (31.23%), Postives = 204/397 (51.39%), Query Frame = 0

Query: 80  QTLGEFPREELAGKVVMVRFDSTLLLLEEMDLKTNS-MKNAVFTIKYLQKSGAKVILASN 139
           +++G+    EL GK V VR D  + L +  ++  ++ ++ AV TIK+L  +GAKVIL+S+
Sbjct: 79  KSVGDLTAAELKGKKVFVRADLNVPLDDNQNITDDTRIRAAVPTIKHLMANGAKVILSSH 138

Query: 140 -WNTNSAARYLDIKSVVDYLSSVLQLKVCPVK-CSSYSMMLTEEGFEKADILLLENLSVF 199
                       +  +V  LS +L ++V  V+ C    +        +  +LLLEN+  +
Sbjct: 139 LGRPKGVTPKYSLAPLVPRLSELLGIQVVKVEDCIGPEVEKLVASLPEGGVLLLENVRFY 198

Query: 200 KEEVSNCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCSTCLAGFNFQEGLCQLK 259
           KEE  N  +F+K L+   D++VND+F  +HR  AST G+T+F    +AGF  Q+ L  L 
Sbjct: 199 KEEEKNEPEFAKKLASLADLYVNDAFGTAHRAHASTEGVTKFLKPSVAGFLLQKELDYLV 258

Query: 260 KAAQTKRQPYVAIIGGGNLLNKLAALHVLASTCSVLVFVGMMSFQIMQALGLSVSYRLMN 319
            A    ++P+ AI+GG  + +K+  +  L   C +L+  G M F   +A GLSV   L+ 
Sbjct: 259 GAVSNPKRPFAAIVGGSKVSSKIGVIESLLEKCDILLLGGGMIFTFYKAQGLSVGSSLVE 318

Query: 320 HGVCKEAAELIQFSLDKRVRIVYPKDFWCAN-VDTSKKMEIFASHDIPDGWLPVDLGPTS 379
               + A  L++ +  K V ++ P D   A+        +I  +  IPDGW+ +D+GP S
Sbjct: 319 EDKLELATSLLEKAKAKGVSLLLPSDVVIADKFAPDANSKIVPASAIPDGWMGLDIGPDS 378

Query: 380 LDEINALLMNSKKIIWIGPVKFSDSSQSARGASRLARKLYDLSQRDCDITVVGTTACKAI 439
           +   N  L  +K +IW GP+   +  + A G   +A+KL DLS +     + G  +  A+
Sbjct: 379 VKTFNDALDTTKTVIWNGPMGVFEFDKFAVGTEAIAKKLADLSGKGVTTIIGGGDSVAAV 438

Query: 440 MQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRA 473
            +        ++        +  +GK LPGV+ALD A
Sbjct: 439 EKVGVASVMSHISTGGGASLELLEGKVLPGVIALDEA 475

BLAST of CSPI03G33330 vs. ExPASy Swiss-Prot
Match: P29409 (Phosphoglycerate kinase, chloroplastic (Fragment) OS=Spinacia oleracea OX=3562 PE=2 SV=2)

HSP 1 Score: 189.5 bits (480), Expect = 1.2e-46
Identity = 121/404 (29.95%), Postives = 207/404 (51.24%), Query Frame = 0

Query: 80  QTLGEFPREELAGKVVMVRFDSTLLLLEEMDLKTNS-MKNAVFTIKYLQKSGAKVILASN 139
           +++G+    +L GK V VR D  + L +  ++  ++ ++ A+ TIK+L  +GAKVIL+S+
Sbjct: 32  KSVGDLTSADLKGKKVFVRADLNVPLDDSQNITDDTRIRAAIPTIKHLINNGAKVILSSH 91

Query: 140 -WNTNSAARYLDIKSVVDYLSSVLQLKVCPV-KCSSYSMMLTEEGFEKADILLLENLSVF 199
                       +  +V  LS +L L+V     C    +        +  +LLLEN+  +
Sbjct: 92  LGRPKGVTPKFSLAPLVPRLSELLGLQVVKADDCIGPDVEKLVAELPEGGVLLLENVRFY 151

Query: 200 KEEVSNCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCSTCLAGFNFQEGLCQLK 259
           KEE  N  +F+K L+   D++VND+F  +HR  AST G+T+F    +AGF  Q+ L  L 
Sbjct: 152 KEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVTKFLKPSVAGFLLQKELDYLV 211

Query: 260 KAAQTKRQPYVAIIGGGNLLNKLAALHVLASTCSVLVFVGMMSFQIMQALGLSVSYRLMN 319
            A    ++P+ AI+GG  + +K+  +  L   C +L+  G M F   +A G+SV   L+ 
Sbjct: 212 GAVSNPKRPFAAIVGGSKVSSKIGVIESLLEKCDILLLGGGMIFTFYKAQGMSVGSSLVE 271

Query: 320 HGVCKEAAELIQFSLDKRVRIVYPKDFWCAN---VDTSKKMEIFASHDIPDGWLPVDLGP 379
                 A  L+  + +K V ++ P D   A+    D   K  I  +  IPDGW+ +D+GP
Sbjct: 272 EDKLDLATSLLAKAKEKGVSLLLPTDVVIADKFAADADSK--IVPASGIPDGWMGLDIGP 331

Query: 380 TSLDEINALLMNSKKIIWIGPVKFSDSSQSARGASRLARKLYDLSQRDCDITVVGTTACK 439
            S+   +  L  ++ +IW GP+   +  + A G   +A+KL ++S++     + G  +  
Sbjct: 332 DSIKTFSEALDTTQTVIWNGPMGVFEFEKFAAGTEAIAKKLEEISKKGATTIIGGGDSVA 391

Query: 440 AIMQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRAYPYEI 478
           A+ +     +  ++        +  +GKQLPGVLAL+ A P  +
Sbjct: 392 AVEKVGVAEAMSHISTGGGASLELLEGKQLPGVLALNEADPVPV 433

BLAST of CSPI03G33330 vs. ExPASy Swiss-Prot
Match: Q9LD57 (Phosphoglycerate kinase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PGK1 PE=1 SV=1)

HSP 1 Score: 188.7 bits (478), Expect = 2.1e-46
Identity = 120/402 (29.85%), Postives = 203/402 (50.50%), Query Frame = 0

Query: 80  QTLGEFPREELAGKVVMVRFDSTLLLLEEMDLKTNS-MKNAVFTIKYLQKSGAKVILASN 139
           +++G+    +L GK V VR D  + L +   +  ++ ++ A+ TIKYL ++GAKVIL+++
Sbjct: 80  KSVGDLTSADLKGKKVFVRADLNVPLDDNQTITDDTRIRAAIPTIKYLIENGAKVILSTH 139

Query: 140 -WNTNSAARYLDIKSVVDYLSSVLQLKVCPV-KCSSYSMMLTEEGFEKADILLLENLSVF 199
                       +  +V  LS +L ++V     C    +        +  +LLLEN+  +
Sbjct: 140 LGRPKGVTPKFSLAPLVPRLSELLGIEVTKADDCIGPEVESLVASLPEGGVLLLENVRFY 199

Query: 200 KEEVSNCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCSTCLAGFNFQEGLCQLK 259
           KEE  N  +F+K L+   D++VND+F  +HR  AST G+T+F    +AGF  Q+ L  L 
Sbjct: 200 KEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVTKFLKPSVAGFLLQKELDYLV 259

Query: 260 KAAQTKRQPYVAIIGGGNLLNKLAALHVLASTCSVLVFVGMMSFQIMQALGLSVSYRLMN 319
            A    ++P+ AI+GG  + +K+  +  L   C +L+  G M F   +A GLSV   L+ 
Sbjct: 260 GAVSNPKRPFAAIVGGSKVSSKIGVIESLLEKCDILLLGGGMIFTFYKAQGLSVGSSLVE 319

Query: 320 HGVCKEAAELIQFSLDKRVRIVYPKDFWCAN-VDTSKKMEIFASHDIPDGWLPVDLGPTS 379
               + A EL+  +  K V ++ P D   A+        +I  +  I DGW+ +D+GP S
Sbjct: 320 EDKLELATELLAKAKAKGVSLLLPTDVVVADKFAPDANSKIVPASGIEDGWMGLDIGPDS 379

Query: 380 LDEINALLMNSKKIIWIGPVKFSDSSQSARGASRLARKLYDLSQRDCDITVVGTTACKAI 439
           +   N  L  ++ +IW GP+   +  + A G   +A KL +LS++     + G  +  A+
Sbjct: 380 IKTFNEALDTTQTVIWNGPMGVFEMEKFAAGTEAIANKLAELSEKGVTTIIGGGDSVAAV 439

Query: 440 MQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRAYPYEI 478
            +        ++        +  +GK LPGV+ALD A P  +
Sbjct: 440 EKVGVAGVMSHISTGGGASLELLEGKVLPGVIALDEAIPVTV 481

BLAST of CSPI03G33330 vs. ExPASy Swiss-Prot
Match: P12783 (Phosphoglycerate kinase, cytosolic OS=Triticum aestivum OX=4565 PE=2 SV=1)

HSP 1 Score: 186.0 bits (471), Expect = 1.4e-45
Identity = 124/398 (31.16%), Postives = 202/398 (50.75%), Query Frame = 0

Query: 79  VQTLGEFPREELAGKVVMVRFDSTLLLLEEMDLKTNS-MKNAVFTIKYLQKSGAKVILAS 138
           V TLGE    +L GK V VR D  + L +   +  ++ ++ ++ TIKYL + GAKVILAS
Sbjct: 7   VGTLGE---ADLKGKKVFVRADLNVPLDDAQKITDDTRIRASIPTIKYLLEKGAKVILAS 66

Query: 139 N-WNTNSAARYLDIKSVVDYLSSVLQLKVCPV-KCSSYSMMLTEEGFEKADILLLENLSV 198
           +            +K +V  LS +L L+V     C    +           +LLLEN+  
Sbjct: 67  HLGRPKGVTPKFSLKPLVARLSELLGLEVVMAPDCIGEEVEKLAAALPDGGVLLLENVRF 126

Query: 199 FKEEVSNCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCSTCLAGFNFQEGLCQL 258
           +KEE  N  +F+K L+   D++VND+F  +HR  AST G+T+F    +AGF  Q+ L  L
Sbjct: 127 YKEEEKNDPEFAKKLASVADLYVNDAFGTAHRAHASTEGVTKFLRPSVAGFLMQKELDYL 186

Query: 259 KKAAQTKRQPYVAIIGGGNLLNKLAALHVLASTCSVLVFVGMMSFQIMQALGLSVSYRLM 318
             A    ++P+ AI+GG  + +K+  +  L +   +L+  G M F   +A GL+V   L+
Sbjct: 187 VGAVANPKKPFAAIVGGSKVSSKIGVIESLLAKVDILILGGGMIFTFYKAQGLAVGKSLV 246

Query: 319 NHGVCKEAAELIQFSLDKRVRIVYPKDFWCAN-VDTSKKMEIFASHDIPDGWLPVDLGPT 378
                + A  LI+ +  K V+++ P D   A+      + +I  +  IPDGW+ +D+GP 
Sbjct: 247 EEDKLELATSLIETAKSKGVKLLLPTDVVVADKFAADAESKIVPATAIPDGWMGLDVGPD 306

Query: 379 SLDEINALLMNSKKIIWIGPVKFSDSSQSARGASRLARKLYDLSQRDCDITVVGTTACKA 438
           S+      L  +K +IW GP+   +  + A G   +A++L +L+ +     + G  +  A
Sbjct: 307 SIKTFAEALDTTKTVIWNGPMGVFEFEKFAAGTDAIAKQLAELTGKGVTTIIGGGDSVAA 366

Query: 439 IMQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRA 473
           + +        ++        +  +GK LPGVLALD A
Sbjct: 367 VEKAGLADKMSHISTGGGASLELLEGKPLPGVLALDEA 401

BLAST of CSPI03G33330 vs. ExPASy Swiss-Prot
Match: P50318 (Phosphoglycerate kinase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At1g56190 PE=1 SV=3)

HSP 1 Score: 184.5 bits (467), Expect = 4.0e-45
Identity = 118/402 (29.35%), Postives = 204/402 (50.75%), Query Frame = 0

Query: 80  QTLGEFPREELAGKVVMVRFDSTLLLLEEMDLKTNS-MKNAVFTIKYLQKSGAKVILASN 139
           +++G+    +L GK V VR D  + L +  ++  ++ ++ A+ TIK+L ++GAKVIL+++
Sbjct: 77  KSVGDLNSVDLKGKKVFVRADLNVPLDDNQNITDDTRIRAAIPTIKFLIENGAKVILSTH 136

Query: 140 -WNTNSAARYLDIKSVVDYLSSVLQLKVCPV-KCSSYSMMLTEEGFEKADILLLENLSVF 199
                       +  +V  LS +L ++V     C    +        +  +LLLEN+  +
Sbjct: 137 LGRPKGVTPKFSLAPLVPRLSELLGIEVVKADDCIGPEVETLVASLPEGGVLLLENVRFY 196

Query: 200 KEEVSNCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCSTCLAGFNFQEGLCQLK 259
           KEE  N   F+K L+   D++VND+F  +HR  AST G+T+F    +AGF  Q+ L  L 
Sbjct: 197 KEEEKNEPDFAKKLASLADLYVNDAFGTAHRAHASTEGVTKFLKPSVAGFLLQKELDYLV 256

Query: 260 KAAQTKRQPYVAIIGGGNLLNKLAALHVLASTCSVLVFVGMMSFQIMQALGLSVSYRLMN 319
            A    ++P+ AI+GG  + +K+  +  L   C +L+  G M F   +A GLSV   L+ 
Sbjct: 257 GAVSNPKRPFAAIVGGSKVSSKIGVIESLLEKCDILLLGGGMIFTFYKAQGLSVGSSLVE 316

Query: 320 HGVCKEAAELIQFSLDKRVRIVYPKDFWCAN-VDTSKKMEIFASHDIPDGWLPVDLGPTS 379
               + A  L+  +  + V ++ P D   A+        +I  +  IPDGW+ +D+GP S
Sbjct: 317 EDKLELATTLLAKAKARGVSLLLPTDVVIADKFAPDANSKIVPASAIPDGWMGLDIGPDS 376

Query: 380 LDEINALLMNSKKIIWIGPVKFSDSSQSARGASRLARKLYDLSQRDCDITVVGTTACKAI 439
           +   N  L  ++ +IW GP+   +  + A+G   +A KL +LS++     + G  +  A+
Sbjct: 377 VKTFNEALDTTQTVIWNGPMGVFEFEKFAKGTEAVANKLAELSKKGVTTIIGGGDSVAAV 436

Query: 440 MQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRAYPYEI 478
            +        ++        +  +GK LPGV+ALD A P  +
Sbjct: 437 EKVGVAGVMSHISTGGGASLELLEGKVLPGVVALDEATPVTV 478

BLAST of CSPI03G33330 vs. ExPASy TrEMBL
Match: A0A0A0LB01 (Phosphoglycerate kinase OS=Cucumis sativus OX=3659 GN=Csa_3G740160 PE=3 SV=1)

HSP 1 Score: 1344.7 bits (3479), Expect = 0.0e+00
Identity = 669/675 (99.11%), Postives = 671/675 (99.41%), Query Frame = 0

Query: 1   MGQLLNPPQGIIFVIKAVAQDPAKLYRQNLSPYFSKYDRYHAVLMSTPARSWGAVYCKNL 60
           MGQLLNPPQGI+FVIKAVAQDPAKLYRQNLSPYFSKYDRYHAVLMSTPARSWGAVYCKNL
Sbjct: 1   MGQLLNPPQGILFVIKAVAQDPAKLYRQNLSPYFSKYDRYHAVLMSTPARSWGAVYCKNL 60

Query: 61  VNSEDHASSWWNLEAVPHVQTLGEFPREELAGKVVMVRFDSTLLLLEEMDLKTNSMKNAV 120
           VNSEDHASSWWNLEAVPHVQTLGEFPREELAGKVVMVRFDSTLLLLEEMDLKTNSMKNAV
Sbjct: 61  VNSEDHASSWWNLEAVPHVQTLGEFPREELAGKVVMVRFDSTLLLLEEMDLKTNSMKNAV 120

Query: 121 FTIKYLQKSGAKVILASNWNTNSAARYLDIKSVVDYLSSVLQLKVCPVKCSSYSMMLTEE 180
           FTIKYL KSGAKVILASNWNTNSAARYLDIKSVVDYLSSVLQLKVCPVKCSSYSMMLTEE
Sbjct: 121 FTIKYLHKSGAKVILASNWNTNSAARYLDIKSVVDYLSSVLQLKVCPVKCSSYSMMLTEE 180

Query: 181 GFEKADILLLENLSVFKEEVSNCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCS 240
           GFEKADILLLENLSVFKEEV+NCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCS
Sbjct: 181 GFEKADILLLENLSVFKEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCS 240

Query: 241 TCLAGFNFQEGLCQLKKAAQTKRQPYVAIIGGGNLLNKLAALHVLASTCSVLVFVGMMSF 300
           TCLAGFNFQEGLCQLKKAAQTKRQPYVAIIGGGNLLNKLAALHVLASTCSVLVFVGMMSF
Sbjct: 241 TCLAGFNFQEGLCQLKKAAQTKRQPYVAIIGGGNLLNKLAALHVLASTCSVLVFVGMMSF 300

Query: 301 QIMQALGLSVSYRLMNHGVCKEAAELIQFSLDKRVRIVYPKDFWCANVDTSKKMEIFASH 360
           QIMQALGLSVSYRLMNHGVCKEAAELIQFSLDKRVRIVYPKDFWCANVDTSKKMEIFASH
Sbjct: 301 QIMQALGLSVSYRLMNHGVCKEAAELIQFSLDKRVRIVYPKDFWCANVDTSKKMEIFASH 360

Query: 361 DIPDGWLPVDLGPTSLDEINALLMNSKKIIWIGPVKFSDSSQSARGASRLARKLYDLSQR 420
           DIPDGWLPVDLGPTSLDEINALLMNSKKIIWIGPVKFSDSSQSARGASRLARKLYDLSQR
Sbjct: 361 DIPDGWLPVDLGPTSLDEINALLMNSKKIIWIGPVKFSDSSQSARGASRLARKLYDLSQR 420

Query: 421 DCDITVVGTTACKAIMQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRAYPYEINWD 480
           DCDITVVGTTACKAIMQESSTL AYNVFENASVVWDFFKGKQLPGVLALDRAYPYEINWD
Sbjct: 421 DCDITVVGTTACKAIMQESSTLCAYNVFENASVVWDFFKGKQLPGVLALDRAYPYEINWD 480

Query: 481 AVFCDTALPLVVDIGSGNGMFLLEMARRRKDHNFLGLEINEKLVKRCLHSVHQLEMKNGH 540
           AVFCDT LPLVVDIGSGNGMFLLEMARRRKDHNFLGLEINEKLVKRCLHSVHQLEMKNGH
Sbjct: 481 AVFCDTTLPLVVDIGSGNGMFLLEMARRRKDHNFLGLEINEKLVKRCLHSVHQLEMKNGH 540

Query: 541 FIATNATSTFRSIVCNYPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLASNGK 600
           FIATNATSTFRSIVCNYPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLASNGK
Sbjct: 541 FIATNATSTFRSIVCNYPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLASNGK 600

Query: 601 VFLQSDVEAVALRMREAFLLYGKGKLGVLQEQVGEEWLSENPFGVRSDWERHVLDRGDPM 660
           VFLQSDVEAVALRMREAFLLYGKGKLGVLQEQVGEEWLSENPFGVRSDWERHVLDRGDPM
Sbjct: 601 VFLQSDVEAVALRMREAFLLYGKGKLGVLQEQVGEEWLSENPFGVRSDWERHVLDRGDPM 660

Query: 661 FRLMLSKSTTTKSFN 676
           FRLMLSKS TTKSFN
Sbjct: 661 FRLMLSKS-TTKSFN 674

BLAST of CSPI03G33330 vs. ExPASy TrEMBL
Match: A0A1S4DUU0 (Phosphoglycerate kinase OS=Cucumis melo OX=3656 GN=LOC103487132 PE=3 SV=1)

HSP 1 Score: 1312.0 bits (3394), Expect = 0.0e+00
Identity = 649/675 (96.15%), Postives = 662/675 (98.07%), Query Frame = 0

Query: 1   MGQLLNPPQGIIFVIKAVAQDPAKLYRQNLSPYFSKYDRYHAVLMSTPARSWGAVYCKNL 60
           MGQLLNP QGI FVIKAVAQDPAKLYRQ LSPYFSKYDRYH VLMSTPARSWGAVYCKNL
Sbjct: 1   MGQLLNPLQGIFFVIKAVAQDPAKLYRQTLSPYFSKYDRYHTVLMSTPARSWGAVYCKNL 60

Query: 61  VNSEDHASSWWNLEAVPHVQTLGEFPREELAGKVVMVRFDSTLLLLEEMDLKTNSMKNAV 120
           VNSEDHASSWWNLEAVPHVQTLGEFPREELAGKVVMVRFDSTLLLLEEMDL+TNSMKNAV
Sbjct: 61  VNSEDHASSWWNLEAVPHVQTLGEFPREELAGKVVMVRFDSTLLLLEEMDLRTNSMKNAV 120

Query: 121 FTIKYLQKSGAKVILASNWNTNSAARYLDIKSVVDYLSSVLQLKVCPVKCSSYSMMLTEE 180
           FTIKYL K GAKVILA+NWNTNSAAR+LDIKSVVDYLSSVLQLKVCPVKCSSYSMMLTEE
Sbjct: 121 FTIKYLHKYGAKVILATNWNTNSAARHLDIKSVVDYLSSVLQLKVCPVKCSSYSMMLTEE 180

Query: 181 GFEKADILLLENLSVFKEEVSNCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCS 240
           GFEKADILLLENLS+FKEEV+NCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCS
Sbjct: 181 GFEKADILLLENLSMFKEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCS 240

Query: 241 TCLAGFNFQEGLCQLKKAAQTKRQPYVAIIGGGNLLNKLAALHVLASTCSVLVFVGMMSF 300
           TCLAGFNFQE LCQLKKAA+TKRQPYVAIIGGGNLL+KLAALHVLASTCS LVFVGMMSF
Sbjct: 241 TCLAGFNFQERLCQLKKAAETKRQPYVAIIGGGNLLDKLAALHVLASTCSALVFVGMMSF 300

Query: 301 QIMQALGLSVSYRLMNHGVCKEAAELIQFSLDKRVRIVYPKDFWCANVDTSKKMEIFASH 360
           QIMQALGLSVSYRLMNHGVCKEAA+LIQFSLDKRV+IVYPKDFWC NVDTSKK+EIFASH
Sbjct: 301 QIMQALGLSVSYRLMNHGVCKEAADLIQFSLDKRVQIVYPKDFWCVNVDTSKKIEIFASH 360

Query: 361 DIPDGWLPVDLGPTSLDEINALLMNSKKIIWIGPVKFSDSSQSARGASRLARKLYDLSQR 420
           DIPDGWLPVDLGPTSLDEINALLMNSKKIIWIGPVKFSDSSQSARGASRLARKLYDL+QR
Sbjct: 361 DIPDGWLPVDLGPTSLDEINALLMNSKKIIWIGPVKFSDSSQSARGASRLARKLYDLAQR 420

Query: 421 DCDITVVGTTACKAIMQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRAYPYEINWD 480
           DCDITVVGTTACKAIMQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRAYPYEINWD
Sbjct: 421 DCDITVVGTTACKAIMQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRAYPYEINWD 480

Query: 481 AVFCDTALPLVVDIGSGNGMFLLEMARRRKDHNFLGLEINEKLVKRCLHSVHQLEMKNGH 540
           AVFCDTALPLVVDIGSGNGMFLLEMARRRKDHNFLGLEINEKLVKRCLHSVHQLEMKNGH
Sbjct: 481 AVFCDTALPLVVDIGSGNGMFLLEMARRRKDHNFLGLEINEKLVKRCLHSVHQLEMKNGH 540

Query: 541 FIATNATSTFRSIVCNYPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLASNGK 600
           FIATNATSTFRSIVC+YPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLASNGK
Sbjct: 541 FIATNATSTFRSIVCSYPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLASNGK 600

Query: 601 VFLQSDVEAVALRMREAFLLYGKGKLGVLQEQVGEEWLSENPFGVRSDWERHVLDRGDPM 660
           VFLQSDVEAVALRMREAFLLYGKGKLGVLQEQ GEEWLSENPFGVRSDWE+HVLDRG PM
Sbjct: 601 VFLQSDVEAVALRMREAFLLYGKGKLGVLQEQFGEEWLSENPFGVRSDWEQHVLDRGHPM 660

Query: 661 FRLMLSKSTTTKSFN 676
           FRLMLS STT KSFN
Sbjct: 661 FRLMLSNSTTNKSFN 675

BLAST of CSPI03G33330 vs. ExPASy TrEMBL
Match: A0A5D3DNE9 (Phosphoglycerate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G002660 PE=3 SV=1)

HSP 1 Score: 1289.6 bits (3336), Expect = 0.0e+00
Identity = 637/661 (96.37%), Postives = 650/661 (98.34%), Query Frame = 0

Query: 1   MGQLLNPPQGIIFVIKAVAQDPAKLYRQNLSPYFSKYDRYHAVLMSTPARSWGAVYCKNL 60
           MGQLLNP QGI FVIKAVAQDPAKLYRQ LSPYFSKYDRYH VLMSTPARSWGAVYCKNL
Sbjct: 29  MGQLLNPLQGIFFVIKAVAQDPAKLYRQTLSPYFSKYDRYHTVLMSTPARSWGAVYCKNL 88

Query: 61  VNSEDHASSWWNLEAVPHVQTLGEFPREELAGKVVMVRFDSTLLLLEEMDLKTNSMKNAV 120
           VNSEDHASSWWNLEAVPHVQTLGEFPREELAGKVVMVRFDSTLLLLEEMDL+TNSMKNAV
Sbjct: 89  VNSEDHASSWWNLEAVPHVQTLGEFPREELAGKVVMVRFDSTLLLLEEMDLRTNSMKNAV 148

Query: 121 FTIKYLQKSGAKVILASNWNTNSAARYLDIKSVVDYLSSVLQLKVCPVKCSSYSMMLTEE 180
           FTIKYL K GAKVILA+NWNTNSAAR+LDIKSVVDYLSSVLQLKVCPVKCSSYSMMLTEE
Sbjct: 149 FTIKYLHKYGAKVILATNWNTNSAARHLDIKSVVDYLSSVLQLKVCPVKCSSYSMMLTEE 208

Query: 181 GFEKADILLLENLSVFKEEVSNCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCS 240
           GFEKADILLLENLS+FKEEV+NCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCS
Sbjct: 209 GFEKADILLLENLSMFKEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCS 268

Query: 241 TCLAGFNFQEGLCQLKKAAQTKRQPYVAIIGGGNLLNKLAALHVLASTCSVLVFVGMMSF 300
           TCLAGFNFQE LCQLKKAA+TKRQPYVAIIGGGNLL+KLAALHVLASTCS LVFVGMMSF
Sbjct: 269 TCLAGFNFQERLCQLKKAAETKRQPYVAIIGGGNLLDKLAALHVLASTCSALVFVGMMSF 328

Query: 301 QIMQALGLSVSYRLMNHGVCKEAAELIQFSLDKRVRIVYPKDFWCANVDTSKKMEIFASH 360
           QIMQALGLSVSYRLMNHGVCKEAA+LIQFSLDKRV+IVYPKDFWC NVDTSKK+EIFASH
Sbjct: 329 QIMQALGLSVSYRLMNHGVCKEAADLIQFSLDKRVQIVYPKDFWCVNVDTSKKIEIFASH 388

Query: 361 DIPDGWLPVDLGPTSLDEINALLMNSKKIIWIGPVKFSDSSQSARGASRLARKLYDLSQR 420
           DIPDGWLPVDLGPTSLDEINALLMNSKKIIWIGPVKFSDSSQSARGASRLARKLYDL+QR
Sbjct: 389 DIPDGWLPVDLGPTSLDEINALLMNSKKIIWIGPVKFSDSSQSARGASRLARKLYDLAQR 448

Query: 421 DCDITVVGTTACKAIMQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRAYPYEINWD 480
           DCDITVVGTTACKAIMQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRAYPYEINWD
Sbjct: 449 DCDITVVGTTACKAIMQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRAYPYEINWD 508

Query: 481 AVFCDTALPLVVDIGSGNGMFLLEMARRRKDHNFLGLEINEKLVKRCLHSVHQLEMKNGH 540
           AVFCDTALPLVVDIGSGNGMFLLEMARRRKDHNFLGLEINEKLVKRCLHSVHQLEMKNGH
Sbjct: 509 AVFCDTALPLVVDIGSGNGMFLLEMARRRKDHNFLGLEINEKLVKRCLHSVHQLEMKNGH 568

Query: 541 FIATNATSTFRSIVCNYPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLASNGK 600
           FIATNATSTFRSIVC+YPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLASNGK
Sbjct: 569 FIATNATSTFRSIVCSYPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLASNGK 628

Query: 601 VFLQSDVEAVALRMREAFLLYGKGKLGVLQEQVGEEWLSENPFGVRSDWERHVLDRGDPM 660
           VFLQSDVEAVALRMREAFLLYGKGKLGVLQEQ GEEWLSENPFGVRSDWE+HVLDRG PM
Sbjct: 629 VFLQSDVEAVALRMREAFLLYGKGKLGVLQEQFGEEWLSENPFGVRSDWEQHVLDRGHPM 688

Query: 661 F 662
           F
Sbjct: 689 F 689

BLAST of CSPI03G33330 vs. ExPASy TrEMBL
Match: A0A5A7TS09 (Phosphoglycerate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold236G003450 PE=3 SV=1)

HSP 1 Score: 1276.2 bits (3301), Expect = 0.0e+00
Identity = 637/685 (92.99%), Postives = 650/685 (94.89%), Query Frame = 0

Query: 1   MGQLLNPPQGIIFVIKAVAQDPAKLYRQNLSPYFSKYDRYHAVLMSTPARSWGAVYCKNL 60
           MGQLLNP QGI FVIKAVAQDPAKLYRQ LSPYFSKYDRYH VLMSTPARSWGAVYCKNL
Sbjct: 29  MGQLLNPLQGIFFVIKAVAQDPAKLYRQTLSPYFSKYDRYHTVLMSTPARSWGAVYCKNL 88

Query: 61  VNSEDHASSWWNLEAVPHVQTLGEFPREELAGKVVMVRFDSTLLLLEEMDLKTNSMKNAV 120
           VNSEDHASSWWNLEAVPHVQTLGEFPREELAGKVVMVRFDSTLLLLEEMDL+TNSMKNAV
Sbjct: 89  VNSEDHASSWWNLEAVPHVQTLGEFPREELAGKVVMVRFDSTLLLLEEMDLRTNSMKNAV 148

Query: 121 FTIKYLQKSGAKVILASNWNTNSAARYLDIKSVVDYLSSVLQLKVCPVKCSSYSMMLTEE 180
           FTIKYL K GAKVILA+NWNTNSAAR+LDIKSVVDYLSSVLQLKVCPVKCSSYSMMLTEE
Sbjct: 149 FTIKYLHKYGAKVILATNWNTNSAARHLDIKSVVDYLSSVLQLKVCPVKCSSYSMMLTEE 208

Query: 181 GFEKADILLLENLSVFKEEVSNCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCS 240
           GFEKADILLLENLS+FKEEV+NCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCS
Sbjct: 209 GFEKADILLLENLSMFKEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCS 268

Query: 241 TCLAGFNFQEGLCQLKKAAQTKRQPYVAIIGGGNLLNKLAALHVLASTCSVLVFVGMMSF 300
           TCLAGFNFQE LCQLKKAA+TKRQPYVAIIGGGNLL+KLAALHVLASTCS LVFVGMMSF
Sbjct: 269 TCLAGFNFQERLCQLKKAAETKRQPYVAIIGGGNLLDKLAALHVLASTCSALVFVGMMSF 328

Query: 301 QIMQALGLSVSYRLMNHGVCKEAAELIQFSLDKRVRIVYPKDFWCANVDTSKKMEIFASH 360
           QIMQALGLSVSYRLMNHGVCKEAA+LIQFSLDKRV+IVYPKDFWC NVDTSKK+EIFASH
Sbjct: 329 QIMQALGLSVSYRLMNHGVCKEAADLIQFSLDKRVQIVYPKDFWCVNVDTSKKIEIFASH 388

Query: 361 DIPDGWLPVDLGPTSLDEINALLMNSKKIIWIGPVKFSDSSQSARGASRLARKLYDLSQR 420
           DIPDGWLPVDLGPTSLDEINALLMNSKKIIWIGPVKFSDSSQSARGASRLARKLYDL+QR
Sbjct: 389 DIPDGWLPVDLGPTSLDEINALLMNSKKIIWIGPVKFSDSSQSARGASRLARKLYDLAQR 448

Query: 421 DCDITVVGTTACKAIMQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRAYPYEINWD 480
           DCDITVVGTTACKAIMQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRAYPYEINWD
Sbjct: 449 DCDITVVGTTACKAIMQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRAYPYEINWD 508

Query: 481 AVFCDTALPLVVDIGSGNGMFLLEMARRRKDHNFLGLEINEK------------------ 540
           AVFCDTALPLVVDIGSGNGMFLLEMARRRKDHNFLGLEINEK                  
Sbjct: 509 AVFCDTALPLVVDIGSGNGMFLLEMARRRKDHNFLGLEINEKVFFLFIFSINFNHIHYLT 568

Query: 541 ------LVKRCLHSVHQLEMKNGHFIATNATSTFRSIVCNYPGELVLVSIQCPNPDFNKP 600
                 LVKRCLHSVHQLEMKNGHFIATNATSTFRSIVC+YPGELVLVSIQCPNPDFNKP
Sbjct: 569 LVRPFQLVKRCLHSVHQLEMKNGHFIATNATSTFRSIVCSYPGELVLVSIQCPNPDFNKP 628

Query: 601 EHRWRMLQRSLVEAVADLLASNGKVFLQSDVEAVALRMREAFLLYGKGKLGVLQEQVGEE 660
           EHRWRMLQRSLVEAVADLLASNGKVFLQSDVEAVALRMREAFLLYGKGKLGVLQEQ GEE
Sbjct: 629 EHRWRMLQRSLVEAVADLLASNGKVFLQSDVEAVALRMREAFLLYGKGKLGVLQEQFGEE 688

Query: 661 WLSENPFGVRSDWERHVLDRGDPMF 662
           WLSENPFGVRSDWE+HVLDRG PMF
Sbjct: 689 WLSENPFGVRSDWEQHVLDRGHPMF 713

BLAST of CSPI03G33330 vs. ExPASy TrEMBL
Match: A0A6J1J0L1 (Phosphoglycerate kinase OS=Cucurbita maxima OX=3661 GN=LOC111481591 PE=3 SV=1)

HSP 1 Score: 1156.7 bits (2991), Expect = 0.0e+00
Identity = 574/671 (85.54%), Postives = 614/671 (91.51%), Query Frame = 0

Query: 1   MGQLLNPPQGIIFVIKAVAQDPAKLYRQNLSPYFSKYDRYHAVLMSTPARSWGAVYC-KN 60
           MGQLLNP +G IFV KAVAQD  KLY QNL PYF+KYDRYHAVL ST AR WG VYC KN
Sbjct: 1   MGQLLNPFKGSIFVSKAVAQDSPKLYYQNLFPYFTKYDRYHAVLASTEARRWGVVYCKKN 60

Query: 61  LVNSEDHASSWWNLEAVPHVQTLGEFPREELAGKVVMVRFDSTLLLLEEMDLKTNSMKNA 120
            VNSEDHA SWWNLEAVPHVQTL EFPREELAGKVV VRFDSTLLL+EE+DL+ N +KNA
Sbjct: 61  TVNSEDHAPSWWNLEAVPHVQTLREFPREELAGKVVTVRFDSTLLLMEEIDLRINLVKNA 120

Query: 121 VFTIKYLQKSGAKVILASNWNTNSAARYLDIKSVVDYLSSVLQLKVCPVKCSSYSMMLTE 180
           +FT+KYL KSGAKVILASNWNTNS  R+ +++SV DYL+SVLQLKV PV+CSS SMM T+
Sbjct: 121 IFTVKYLHKSGAKVILASNWNTNSITRHPNMESVADYLTSVLQLKVLPVQCSSISMMSTK 180

Query: 181 EGFEKADILLLENLSVFKEEVSNCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFC 240
           E FEK DILLL+NLS FKEEV+NCSKFSK+LSLGVDIFVNDSFSQSHRILASTVGITRFC
Sbjct: 181 ECFEKVDILLLQNLSEFKEEVANCSKFSKLLSLGVDIFVNDSFSQSHRILASTVGITRFC 240

Query: 241 STCLAGFNFQEGLCQLKKAAQTKRQPYVAIIGGGNLLNKLAALHVLASTCSVLVFVGMMS 300
            TC+AGF+FQE LCQLK+AAQTKRQPYVAIIGGGNLL+K++ALH+LASTCS LVFVGMMS
Sbjct: 241 CTCIAGFSFQESLCQLKEAAQTKRQPYVAIIGGGNLLDKVSALHLLASTCSALVFVGMMS 300

Query: 301 FQIMQALGLSVSYRLMNHGVCKEAAELIQFSLDKRVRIVYPKDFWCANVDTSKKMEIFAS 360
           FQIM ALGL VS RL+NH VCKEAA++ QFSLDK V+IVYPKDFWC NVDTSKKMEIFAS
Sbjct: 301 FQIMHALGLPVSSRLVNHSVCKEAADVFQFSLDKSVQIVYPKDFWCKNVDTSKKMEIFAS 360

Query: 361 HDIPDGWLPVDLGPTSLDEINALLMNSKKIIWIGPVKFSDSSQSARGASRLARKLYDLSQ 420
           HDIPDGWLPVDLGPTSLDEINALLM SKKIIWIGPVKF +SSQS  GAS LARKLYDL Q
Sbjct: 361 HDIPDGWLPVDLGPTSLDEINALLMKSKKIIWIGPVKFFNSSQSTGGASILARKLYDLIQ 420

Query: 421 RDCDITVVGTTACKAIMQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRAYPYEINW 480
            DCD+TVVGTTACK++MQESSTL A NVFENASVVWDF KGKQLPGVLALDRAYPY+INW
Sbjct: 421 SDCDVTVVGTTACKSLMQESSTLPACNVFENASVVWDFLKGKQLPGVLALDRAYPYKINW 480

Query: 481 DAVFCDTALPLVVDIGSGNGMFLLEMARRRKDHNFLGLEINEKLVKRCLHSVHQLEMKNG 540
           DAVFCD ALPLVVDIGSGNGMFLLEMARRRKD NFLGLEINEKLVKRCLHSV QLEM+NG
Sbjct: 481 DAVFCDIALPLVVDIGSGNGMFLLEMARRRKDLNFLGLEINEKLVKRCLHSVCQLEMRNG 540

Query: 541 HFIATNATSTFRSIVCNYPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLASNG 600
           HFIATNATSTFRSIV +YPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLA+NG
Sbjct: 541 HFIATNATSTFRSIVSSYPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLAANG 600

Query: 601 KVFLQSDVEAVALRMREAFLLYGKGKLGVLQEQVGEEWLSENPFGVRSDWERHVLDRGDP 660
           KVFLQSDVEAVALRM+EAFLLYGKGKL V Q+QVGE WLSENPFGVRSDWERHVLDRGDP
Sbjct: 601 KVFLQSDVEAVALRMKEAFLLYGKGKLSVFQDQVGEGWLSENPFGVRSDWERHVLDRGDP 660

Query: 661 MFRLMLSKSTT 671
           MFR+MLS STT
Sbjct: 661 MFRIMLSNSTT 671

BLAST of CSPI03G33330 vs. NCBI nr
Match: XP_004137833.1 (phosphoglycerate kinase, cytosolic isoform X1 [Cucumis sativus] >KGN58973.1 hypothetical protein Csa_001234 [Cucumis sativus])

HSP 1 Score: 1344.7 bits (3479), Expect = 0.0e+00
Identity = 669/675 (99.11%), Postives = 671/675 (99.41%), Query Frame = 0

Query: 1   MGQLLNPPQGIIFVIKAVAQDPAKLYRQNLSPYFSKYDRYHAVLMSTPARSWGAVYCKNL 60
           MGQLLNPPQGI+FVIKAVAQDPAKLYRQNLSPYFSKYDRYHAVLMSTPARSWGAVYCKNL
Sbjct: 1   MGQLLNPPQGILFVIKAVAQDPAKLYRQNLSPYFSKYDRYHAVLMSTPARSWGAVYCKNL 60

Query: 61  VNSEDHASSWWNLEAVPHVQTLGEFPREELAGKVVMVRFDSTLLLLEEMDLKTNSMKNAV 120
           VNSEDHASSWWNLEAVPHVQTLGEFPREELAGKVVMVRFDSTLLLLEEMDLKTNSMKNAV
Sbjct: 61  VNSEDHASSWWNLEAVPHVQTLGEFPREELAGKVVMVRFDSTLLLLEEMDLKTNSMKNAV 120

Query: 121 FTIKYLQKSGAKVILASNWNTNSAARYLDIKSVVDYLSSVLQLKVCPVKCSSYSMMLTEE 180
           FTIKYL KSGAKVILASNWNTNSAARYLDIKSVVDYLSSVLQLKVCPVKCSSYSMMLTEE
Sbjct: 121 FTIKYLHKSGAKVILASNWNTNSAARYLDIKSVVDYLSSVLQLKVCPVKCSSYSMMLTEE 180

Query: 181 GFEKADILLLENLSVFKEEVSNCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCS 240
           GFEKADILLLENLSVFKEEV+NCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCS
Sbjct: 181 GFEKADILLLENLSVFKEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCS 240

Query: 241 TCLAGFNFQEGLCQLKKAAQTKRQPYVAIIGGGNLLNKLAALHVLASTCSVLVFVGMMSF 300
           TCLAGFNFQEGLCQLKKAAQTKRQPYVAIIGGGNLLNKLAALHVLASTCSVLVFVGMMSF
Sbjct: 241 TCLAGFNFQEGLCQLKKAAQTKRQPYVAIIGGGNLLNKLAALHVLASTCSVLVFVGMMSF 300

Query: 301 QIMQALGLSVSYRLMNHGVCKEAAELIQFSLDKRVRIVYPKDFWCANVDTSKKMEIFASH 360
           QIMQALGLSVSYRLMNHGVCKEAAELIQFSLDKRVRIVYPKDFWCANVDTSKKMEIFASH
Sbjct: 301 QIMQALGLSVSYRLMNHGVCKEAAELIQFSLDKRVRIVYPKDFWCANVDTSKKMEIFASH 360

Query: 361 DIPDGWLPVDLGPTSLDEINALLMNSKKIIWIGPVKFSDSSQSARGASRLARKLYDLSQR 420
           DIPDGWLPVDLGPTSLDEINALLMNSKKIIWIGPVKFSDSSQSARGASRLARKLYDLSQR
Sbjct: 361 DIPDGWLPVDLGPTSLDEINALLMNSKKIIWIGPVKFSDSSQSARGASRLARKLYDLSQR 420

Query: 421 DCDITVVGTTACKAIMQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRAYPYEINWD 480
           DCDITVVGTTACKAIMQESSTL AYNVFENASVVWDFFKGKQLPGVLALDRAYPYEINWD
Sbjct: 421 DCDITVVGTTACKAIMQESSTLCAYNVFENASVVWDFFKGKQLPGVLALDRAYPYEINWD 480

Query: 481 AVFCDTALPLVVDIGSGNGMFLLEMARRRKDHNFLGLEINEKLVKRCLHSVHQLEMKNGH 540
           AVFCDT LPLVVDIGSGNGMFLLEMARRRKDHNFLGLEINEKLVKRCLHSVHQLEMKNGH
Sbjct: 481 AVFCDTTLPLVVDIGSGNGMFLLEMARRRKDHNFLGLEINEKLVKRCLHSVHQLEMKNGH 540

Query: 541 FIATNATSTFRSIVCNYPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLASNGK 600
           FIATNATSTFRSIVCNYPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLASNGK
Sbjct: 541 FIATNATSTFRSIVCNYPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLASNGK 600

Query: 601 VFLQSDVEAVALRMREAFLLYGKGKLGVLQEQVGEEWLSENPFGVRSDWERHVLDRGDPM 660
           VFLQSDVEAVALRMREAFLLYGKGKLGVLQEQVGEEWLSENPFGVRSDWERHVLDRGDPM
Sbjct: 601 VFLQSDVEAVALRMREAFLLYGKGKLGVLQEQVGEEWLSENPFGVRSDWERHVLDRGDPM 660

Query: 661 FRLMLSKSTTTKSFN 676
           FRLMLSKS TTKSFN
Sbjct: 661 FRLMLSKS-TTKSFN 674

BLAST of CSPI03G33330 vs. NCBI nr
Match: XP_016899748.1 (PREDICTED: phosphoglycerate kinase, cytosolic-like isoform X1 [Cucumis melo] >XP_016899749.1 PREDICTED: phosphoglycerate kinase, cytosolic-like isoform X1 [Cucumis melo])

HSP 1 Score: 1312.0 bits (3394), Expect = 0.0e+00
Identity = 649/675 (96.15%), Postives = 662/675 (98.07%), Query Frame = 0

Query: 1   MGQLLNPPQGIIFVIKAVAQDPAKLYRQNLSPYFSKYDRYHAVLMSTPARSWGAVYCKNL 60
           MGQLLNP QGI FVIKAVAQDPAKLYRQ LSPYFSKYDRYH VLMSTPARSWGAVYCKNL
Sbjct: 1   MGQLLNPLQGIFFVIKAVAQDPAKLYRQTLSPYFSKYDRYHTVLMSTPARSWGAVYCKNL 60

Query: 61  VNSEDHASSWWNLEAVPHVQTLGEFPREELAGKVVMVRFDSTLLLLEEMDLKTNSMKNAV 120
           VNSEDHASSWWNLEAVPHVQTLGEFPREELAGKVVMVRFDSTLLLLEEMDL+TNSMKNAV
Sbjct: 61  VNSEDHASSWWNLEAVPHVQTLGEFPREELAGKVVMVRFDSTLLLLEEMDLRTNSMKNAV 120

Query: 121 FTIKYLQKSGAKVILASNWNTNSAARYLDIKSVVDYLSSVLQLKVCPVKCSSYSMMLTEE 180
           FTIKYL K GAKVILA+NWNTNSAAR+LDIKSVVDYLSSVLQLKVCPVKCSSYSMMLTEE
Sbjct: 121 FTIKYLHKYGAKVILATNWNTNSAARHLDIKSVVDYLSSVLQLKVCPVKCSSYSMMLTEE 180

Query: 181 GFEKADILLLENLSVFKEEVSNCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCS 240
           GFEKADILLLENLS+FKEEV+NCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCS
Sbjct: 181 GFEKADILLLENLSMFKEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCS 240

Query: 241 TCLAGFNFQEGLCQLKKAAQTKRQPYVAIIGGGNLLNKLAALHVLASTCSVLVFVGMMSF 300
           TCLAGFNFQE LCQLKKAA+TKRQPYVAIIGGGNLL+KLAALHVLASTCS LVFVGMMSF
Sbjct: 241 TCLAGFNFQERLCQLKKAAETKRQPYVAIIGGGNLLDKLAALHVLASTCSALVFVGMMSF 300

Query: 301 QIMQALGLSVSYRLMNHGVCKEAAELIQFSLDKRVRIVYPKDFWCANVDTSKKMEIFASH 360
           QIMQALGLSVSYRLMNHGVCKEAA+LIQFSLDKRV+IVYPKDFWC NVDTSKK+EIFASH
Sbjct: 301 QIMQALGLSVSYRLMNHGVCKEAADLIQFSLDKRVQIVYPKDFWCVNVDTSKKIEIFASH 360

Query: 361 DIPDGWLPVDLGPTSLDEINALLMNSKKIIWIGPVKFSDSSQSARGASRLARKLYDLSQR 420
           DIPDGWLPVDLGPTSLDEINALLMNSKKIIWIGPVKFSDSSQSARGASRLARKLYDL+QR
Sbjct: 361 DIPDGWLPVDLGPTSLDEINALLMNSKKIIWIGPVKFSDSSQSARGASRLARKLYDLAQR 420

Query: 421 DCDITVVGTTACKAIMQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRAYPYEINWD 480
           DCDITVVGTTACKAIMQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRAYPYEINWD
Sbjct: 421 DCDITVVGTTACKAIMQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRAYPYEINWD 480

Query: 481 AVFCDTALPLVVDIGSGNGMFLLEMARRRKDHNFLGLEINEKLVKRCLHSVHQLEMKNGH 540
           AVFCDTALPLVVDIGSGNGMFLLEMARRRKDHNFLGLEINEKLVKRCLHSVHQLEMKNGH
Sbjct: 481 AVFCDTALPLVVDIGSGNGMFLLEMARRRKDHNFLGLEINEKLVKRCLHSVHQLEMKNGH 540

Query: 541 FIATNATSTFRSIVCNYPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLASNGK 600
           FIATNATSTFRSIVC+YPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLASNGK
Sbjct: 541 FIATNATSTFRSIVCSYPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLASNGK 600

Query: 601 VFLQSDVEAVALRMREAFLLYGKGKLGVLQEQVGEEWLSENPFGVRSDWERHVLDRGDPM 660
           VFLQSDVEAVALRMREAFLLYGKGKLGVLQEQ GEEWLSENPFGVRSDWE+HVLDRG PM
Sbjct: 601 VFLQSDVEAVALRMREAFLLYGKGKLGVLQEQFGEEWLSENPFGVRSDWEQHVLDRGHPM 660

Query: 661 FRLMLSKSTTTKSFN 676
           FRLMLS STT KSFN
Sbjct: 661 FRLMLSNSTTNKSFN 675

BLAST of CSPI03G33330 vs. NCBI nr
Match: TYK25141.1 (phosphoglycerate kinase, cytosolic-like isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 1289.6 bits (3336), Expect = 0.0e+00
Identity = 637/661 (96.37%), Postives = 650/661 (98.34%), Query Frame = 0

Query: 1   MGQLLNPPQGIIFVIKAVAQDPAKLYRQNLSPYFSKYDRYHAVLMSTPARSWGAVYCKNL 60
           MGQLLNP QGI FVIKAVAQDPAKLYRQ LSPYFSKYDRYH VLMSTPARSWGAVYCKNL
Sbjct: 29  MGQLLNPLQGIFFVIKAVAQDPAKLYRQTLSPYFSKYDRYHTVLMSTPARSWGAVYCKNL 88

Query: 61  VNSEDHASSWWNLEAVPHVQTLGEFPREELAGKVVMVRFDSTLLLLEEMDLKTNSMKNAV 120
           VNSEDHASSWWNLEAVPHVQTLGEFPREELAGKVVMVRFDSTLLLLEEMDL+TNSMKNAV
Sbjct: 89  VNSEDHASSWWNLEAVPHVQTLGEFPREELAGKVVMVRFDSTLLLLEEMDLRTNSMKNAV 148

Query: 121 FTIKYLQKSGAKVILASNWNTNSAARYLDIKSVVDYLSSVLQLKVCPVKCSSYSMMLTEE 180
           FTIKYL K GAKVILA+NWNTNSAAR+LDIKSVVDYLSSVLQLKVCPVKCSSYSMMLTEE
Sbjct: 149 FTIKYLHKYGAKVILATNWNTNSAARHLDIKSVVDYLSSVLQLKVCPVKCSSYSMMLTEE 208

Query: 181 GFEKADILLLENLSVFKEEVSNCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCS 240
           GFEKADILLLENLS+FKEEV+NCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCS
Sbjct: 209 GFEKADILLLENLSMFKEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCS 268

Query: 241 TCLAGFNFQEGLCQLKKAAQTKRQPYVAIIGGGNLLNKLAALHVLASTCSVLVFVGMMSF 300
           TCLAGFNFQE LCQLKKAA+TKRQPYVAIIGGGNLL+KLAALHVLASTCS LVFVGMMSF
Sbjct: 269 TCLAGFNFQERLCQLKKAAETKRQPYVAIIGGGNLLDKLAALHVLASTCSALVFVGMMSF 328

Query: 301 QIMQALGLSVSYRLMNHGVCKEAAELIQFSLDKRVRIVYPKDFWCANVDTSKKMEIFASH 360
           QIMQALGLSVSYRLMNHGVCKEAA+LIQFSLDKRV+IVYPKDFWC NVDTSKK+EIFASH
Sbjct: 329 QIMQALGLSVSYRLMNHGVCKEAADLIQFSLDKRVQIVYPKDFWCVNVDTSKKIEIFASH 388

Query: 361 DIPDGWLPVDLGPTSLDEINALLMNSKKIIWIGPVKFSDSSQSARGASRLARKLYDLSQR 420
           DIPDGWLPVDLGPTSLDEINALLMNSKKIIWIGPVKFSDSSQSARGASRLARKLYDL+QR
Sbjct: 389 DIPDGWLPVDLGPTSLDEINALLMNSKKIIWIGPVKFSDSSQSARGASRLARKLYDLAQR 448

Query: 421 DCDITVVGTTACKAIMQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRAYPYEINWD 480
           DCDITVVGTTACKAIMQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRAYPYEINWD
Sbjct: 449 DCDITVVGTTACKAIMQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRAYPYEINWD 508

Query: 481 AVFCDTALPLVVDIGSGNGMFLLEMARRRKDHNFLGLEINEKLVKRCLHSVHQLEMKNGH 540
           AVFCDTALPLVVDIGSGNGMFLLEMARRRKDHNFLGLEINEKLVKRCLHSVHQLEMKNGH
Sbjct: 509 AVFCDTALPLVVDIGSGNGMFLLEMARRRKDHNFLGLEINEKLVKRCLHSVHQLEMKNGH 568

Query: 541 FIATNATSTFRSIVCNYPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLASNGK 600
           FIATNATSTFRSIVC+YPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLASNGK
Sbjct: 569 FIATNATSTFRSIVCSYPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLASNGK 628

Query: 601 VFLQSDVEAVALRMREAFLLYGKGKLGVLQEQVGEEWLSENPFGVRSDWERHVLDRGDPM 660
           VFLQSDVEAVALRMREAFLLYGKGKLGVLQEQ GEEWLSENPFGVRSDWE+HVLDRG PM
Sbjct: 629 VFLQSDVEAVALRMREAFLLYGKGKLGVLQEQFGEEWLSENPFGVRSDWEQHVLDRGHPM 688

Query: 661 F 662
           F
Sbjct: 689 F 689

BLAST of CSPI03G33330 vs. NCBI nr
Match: KAA0043999.1 (phosphoglycerate kinase, cytosolic-like isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 1276.2 bits (3301), Expect = 0.0e+00
Identity = 637/685 (92.99%), Postives = 650/685 (94.89%), Query Frame = 0

Query: 1   MGQLLNPPQGIIFVIKAVAQDPAKLYRQNLSPYFSKYDRYHAVLMSTPARSWGAVYCKNL 60
           MGQLLNP QGI FVIKAVAQDPAKLYRQ LSPYFSKYDRYH VLMSTPARSWGAVYCKNL
Sbjct: 29  MGQLLNPLQGIFFVIKAVAQDPAKLYRQTLSPYFSKYDRYHTVLMSTPARSWGAVYCKNL 88

Query: 61  VNSEDHASSWWNLEAVPHVQTLGEFPREELAGKVVMVRFDSTLLLLEEMDLKTNSMKNAV 120
           VNSEDHASSWWNLEAVPHVQTLGEFPREELAGKVVMVRFDSTLLLLEEMDL+TNSMKNAV
Sbjct: 89  VNSEDHASSWWNLEAVPHVQTLGEFPREELAGKVVMVRFDSTLLLLEEMDLRTNSMKNAV 148

Query: 121 FTIKYLQKSGAKVILASNWNTNSAARYLDIKSVVDYLSSVLQLKVCPVKCSSYSMMLTEE 180
           FTIKYL K GAKVILA+NWNTNSAAR+LDIKSVVDYLSSVLQLKVCPVKCSSYSMMLTEE
Sbjct: 149 FTIKYLHKYGAKVILATNWNTNSAARHLDIKSVVDYLSSVLQLKVCPVKCSSYSMMLTEE 208

Query: 181 GFEKADILLLENLSVFKEEVSNCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCS 240
           GFEKADILLLENLS+FKEEV+NCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCS
Sbjct: 209 GFEKADILLLENLSMFKEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCS 268

Query: 241 TCLAGFNFQEGLCQLKKAAQTKRQPYVAIIGGGNLLNKLAALHVLASTCSVLVFVGMMSF 300
           TCLAGFNFQE LCQLKKAA+TKRQPYVAIIGGGNLL+KLAALHVLASTCS LVFVGMMSF
Sbjct: 269 TCLAGFNFQERLCQLKKAAETKRQPYVAIIGGGNLLDKLAALHVLASTCSALVFVGMMSF 328

Query: 301 QIMQALGLSVSYRLMNHGVCKEAAELIQFSLDKRVRIVYPKDFWCANVDTSKKMEIFASH 360
           QIMQALGLSVSYRLMNHGVCKEAA+LIQFSLDKRV+IVYPKDFWC NVDTSKK+EIFASH
Sbjct: 329 QIMQALGLSVSYRLMNHGVCKEAADLIQFSLDKRVQIVYPKDFWCVNVDTSKKIEIFASH 388

Query: 361 DIPDGWLPVDLGPTSLDEINALLMNSKKIIWIGPVKFSDSSQSARGASRLARKLYDLSQR 420
           DIPDGWLPVDLGPTSLDEINALLMNSKKIIWIGPVKFSDSSQSARGASRLARKLYDL+QR
Sbjct: 389 DIPDGWLPVDLGPTSLDEINALLMNSKKIIWIGPVKFSDSSQSARGASRLARKLYDLAQR 448

Query: 421 DCDITVVGTTACKAIMQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRAYPYEINWD 480
           DCDITVVGTTACKAIMQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRAYPYEINWD
Sbjct: 449 DCDITVVGTTACKAIMQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRAYPYEINWD 508

Query: 481 AVFCDTALPLVVDIGSGNGMFLLEMARRRKDHNFLGLEINEK------------------ 540
           AVFCDTALPLVVDIGSGNGMFLLEMARRRKDHNFLGLEINEK                  
Sbjct: 509 AVFCDTALPLVVDIGSGNGMFLLEMARRRKDHNFLGLEINEKVFFLFIFSINFNHIHYLT 568

Query: 541 ------LVKRCLHSVHQLEMKNGHFIATNATSTFRSIVCNYPGELVLVSIQCPNPDFNKP 600
                 LVKRCLHSVHQLEMKNGHFIATNATSTFRSIVC+YPGELVLVSIQCPNPDFNKP
Sbjct: 569 LVRPFQLVKRCLHSVHQLEMKNGHFIATNATSTFRSIVCSYPGELVLVSIQCPNPDFNKP 628

Query: 601 EHRWRMLQRSLVEAVADLLASNGKVFLQSDVEAVALRMREAFLLYGKGKLGVLQEQVGEE 660
           EHRWRMLQRSLVEAVADLLASNGKVFLQSDVEAVALRMREAFLLYGKGKLGVLQEQ GEE
Sbjct: 629 EHRWRMLQRSLVEAVADLLASNGKVFLQSDVEAVALRMREAFLLYGKGKLGVLQEQFGEE 688

Query: 661 WLSENPFGVRSDWERHVLDRGDPMF 662
           WLSENPFGVRSDWE+HVLDRG PMF
Sbjct: 689 WLSENPFGVRSDWEQHVLDRGHPMF 713

BLAST of CSPI03G33330 vs. NCBI nr
Match: XP_038904993.1 (phosphoglycerate kinase, cytosolic-like isoform X1 [Benincasa hispida] >XP_038904994.1 phosphoglycerate kinase, cytosolic-like isoform X1 [Benincasa hispida] >XP_038904995.1 phosphoglycerate kinase, cytosolic-like isoform X1 [Benincasa hispida] >XP_038904996.1 phosphoglycerate kinase, cytosolic-like isoform X1 [Benincasa hispida])

HSP 1 Score: 1208.7 bits (3126), Expect = 0.0e+00
Identity = 598/675 (88.59%), Postives = 631/675 (93.48%), Query Frame = 0

Query: 1   MGQLLNPPQGIIFVIKAVAQDPAKLYRQNLSPYFSKYDRYHAVLMSTPARSWGAVYCKNL 60
           MGQLLNP QG  F+IKA AQDP KLYR+NLSP+FSKYDRY AVLMST AR WG VYCKN 
Sbjct: 1   MGQLLNPLQGSTFIIKAFAQDPPKLYRKNLSPHFSKYDRYRAVLMSTLARRWGVVYCKNF 60

Query: 61  VNSEDHASSWWNLEAVPHVQTLGEFPREELAGKVVMVRFDSTLLLLEEMDLKTNSMKNAV 120
           VN EDHA  WWNLEA+PHVQTLGEFPREEL GKVVMVRFDSTLLLLEEMDL+ NS+KNAV
Sbjct: 61  VNLEDHAPGWWNLEALPHVQTLGEFPREELPGKVVMVRFDSTLLLLEEMDLRINSVKNAV 120

Query: 121 FTIKYLQKSGAKVILASNWNTNSAARYLDIKSVVDYLSSVLQLKVCPVKCSSYSMMLTEE 180
           FTIKYL KS AKVILASNWNTNS  R+ DIKSV DYLSSVLQLKV PVKCSS+SMM TEE
Sbjct: 121 FTIKYLHKSAAKVILASNWNTNSTPRHPDIKSVADYLSSVLQLKVLPVKCSSFSMMSTEE 180

Query: 181 GFEKADILLLENLSVFKEEVSNCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCS 240
            FEKADIL+L+NLSVFKEEV+NCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGI+RFCS
Sbjct: 181 CFEKADILILKNLSVFKEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGISRFCS 240

Query: 241 TCLAGFNFQEGLCQLKKAAQTKRQPYVAIIGGGNLLNKLAALHVLASTCSVLVFVGMMSF 300
           TCLAGFNFQEGL  L+KAA+TKRQPYVAIIGGGNLL+K AALH+LASTCS LVFVG+MS 
Sbjct: 241 TCLAGFNFQEGLGLLRKAAETKRQPYVAIIGGGNLLDKSAALHLLASTCSALVFVGLMSI 300

Query: 301 QIMQALGLSVSYRLMNHGVCKEAAELIQFSLDKRVRIVYPKDFWCANVDTSKKMEIFASH 360
           QIMQALGLSVS RL+NHGVCKEAA++IQFSLDK V+IVYPKDFWC NVD SKKME+FAS+
Sbjct: 301 QIMQALGLSVSSRLVNHGVCKEAADIIQFSLDKNVQIVYPKDFWCKNVDASKKMELFASN 360

Query: 361 DIPDGWLPVDLGPTSLDEINALLMNSKKIIWIGPVKFSDSSQSARGASRLARKLYDLSQR 420
           DIPDGWLPVDLGPTSLDEINALLMNSKKIIWIGPVKFSDSSQS  G S+LARKLYDL+QR
Sbjct: 361 DIPDGWLPVDLGPTSLDEINALLMNSKKIIWIGPVKFSDSSQSTCGTSKLARKLYDLTQR 420

Query: 421 DCDITVVGTTACKAIMQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRAYPYEINWD 480
            CD+TVVGTTACKA+MQESSTLSAYNVFENASVVWDFFKGK LPGVLALDRAYPY+I+WD
Sbjct: 421 GCDVTVVGTTACKAMMQESSTLSAYNVFENASVVWDFFKGKPLPGVLALDRAYPYKIDWD 480

Query: 481 AVFCDTALPLVVDIGSGNGMFLLEMARRRKDHNFLGLEINEKLVKRCLHSVHQLEMKNGH 540
           AVFCDTALPLVVDIGSGNGMFLLEMA RRKD NFLGLEINEKLVKRCLHSV+QLEMKNGH
Sbjct: 481 AVFCDTALPLVVDIGSGNGMFLLEMATRRKDLNFLGLEINEKLVKRCLHSVYQLEMKNGH 540

Query: 541 FIATNATSTFRSIVCNYPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLASNGK 600
           FIATNATSTFRSIV +YPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLASNGK
Sbjct: 541 FIATNATSTFRSIVSSYPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLASNGK 600

Query: 601 VFLQSDVEAVALRMREAFLLYGKGKLGVLQEQVGEEWLSENPFGVRSDWERHVLDRGDPM 660
           VFLQSDVE VALRM+EAFLLYGKGKL +LQEQVGEEWLSENPFGVRSDWERHVLDRGDPM
Sbjct: 601 VFLQSDVEVVALRMKEAFLLYGKGKLAILQEQVGEEWLSENPFGVRSDWERHVLDRGDPM 660

Query: 661 FRLMLSKSTTTKSFN 676
           FRLMLSKSTTT SFN
Sbjct: 661 FRLMLSKSTTTNSFN 675

BLAST of CSPI03G33330 vs. TAIR 10
Match: AT3G12780.1 (phosphoglycerate kinase 1 )

HSP 1 Score: 188.7 bits (478), Expect = 1.5e-47
Identity = 120/402 (29.85%), Postives = 203/402 (50.50%), Query Frame = 0

Query: 80  QTLGEFPREELAGKVVMVRFDSTLLLLEEMDLKTNS-MKNAVFTIKYLQKSGAKVILASN 139
           +++G+    +L GK V VR D  + L +   +  ++ ++ A+ TIKYL ++GAKVIL+++
Sbjct: 80  KSVGDLTSADLKGKKVFVRADLNVPLDDNQTITDDTRIRAAIPTIKYLIENGAKVILSTH 139

Query: 140 -WNTNSAARYLDIKSVVDYLSSVLQLKVCPV-KCSSYSMMLTEEGFEKADILLLENLSVF 199
                       +  +V  LS +L ++V     C    +        +  +LLLEN+  +
Sbjct: 140 LGRPKGVTPKFSLAPLVPRLSELLGIEVTKADDCIGPEVESLVASLPEGGVLLLENVRFY 199

Query: 200 KEEVSNCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCSTCLAGFNFQEGLCQLK 259
           KEE  N  +F+K L+   D++VND+F  +HR  AST G+T+F    +AGF  Q+ L  L 
Sbjct: 200 KEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVTKFLKPSVAGFLLQKELDYLV 259

Query: 260 KAAQTKRQPYVAIIGGGNLLNKLAALHVLASTCSVLVFVGMMSFQIMQALGLSVSYRLMN 319
            A    ++P+ AI+GG  + +K+  +  L   C +L+  G M F   +A GLSV   L+ 
Sbjct: 260 GAVSNPKRPFAAIVGGSKVSSKIGVIESLLEKCDILLLGGGMIFTFYKAQGLSVGSSLVE 319

Query: 320 HGVCKEAAELIQFSLDKRVRIVYPKDFWCAN-VDTSKKMEIFASHDIPDGWLPVDLGPTS 379
               + A EL+  +  K V ++ P D   A+        +I  +  I DGW+ +D+GP S
Sbjct: 320 EDKLELATELLAKAKAKGVSLLLPTDVVVADKFAPDANSKIVPASGIEDGWMGLDIGPDS 379

Query: 380 LDEINALLMNSKKIIWIGPVKFSDSSQSARGASRLARKLYDLSQRDCDITVVGTTACKAI 439
           +   N  L  ++ +IW GP+   +  + A G   +A KL +LS++     + G  +  A+
Sbjct: 380 IKTFNEALDTTQTVIWNGPMGVFEMEKFAAGTEAIANKLAELSEKGVTTIIGGGDSVAAV 439

Query: 440 MQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRAYPYEI 478
            +        ++        +  +GK LPGV+ALD A P  +
Sbjct: 440 EKVGVAGVMSHISTGGGASLELLEGKVLPGVIALDEAIPVTV 481

BLAST of CSPI03G33330 vs. TAIR 10
Match: AT1G56190.1 (Phosphoglycerate kinase family protein )

HSP 1 Score: 184.5 bits (467), Expect = 2.8e-46
Identity = 118/402 (29.35%), Postives = 204/402 (50.75%), Query Frame = 0

Query: 80  QTLGEFPREELAGKVVMVRFDSTLLLLEEMDLKTNS-MKNAVFTIKYLQKSGAKVILASN 139
           +++G+    +L GK V VR D  + L +  ++  ++ ++ A+ TIK+L ++GAKVIL+++
Sbjct: 77  KSVGDLNSVDLKGKKVFVRADLNVPLDDNQNITDDTRIRAAIPTIKFLIENGAKVILSTH 136

Query: 140 -WNTNSAARYLDIKSVVDYLSSVLQLKVCPV-KCSSYSMMLTEEGFEKADILLLENLSVF 199
                       +  +V  LS +L ++V     C    +        +  +LLLEN+  +
Sbjct: 137 LGRPKGVTPKFSLAPLVPRLSELLGIEVVKADDCIGPEVETLVASLPEGGVLLLENVRFY 196

Query: 200 KEEVSNCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCSTCLAGFNFQEGLCQLK 259
           KEE  N   F+K L+   D++VND+F  +HR  AST G+T+F    +AGF  Q+ L  L 
Sbjct: 197 KEEEKNEPDFAKKLASLADLYVNDAFGTAHRAHASTEGVTKFLKPSVAGFLLQKELDYLV 256

Query: 260 KAAQTKRQPYVAIIGGGNLLNKLAALHVLASTCSVLVFVGMMSFQIMQALGLSVSYRLMN 319
            A    ++P+ AI+GG  + +K+  +  L   C +L+  G M F   +A GLSV   L+ 
Sbjct: 257 GAVSNPKRPFAAIVGGSKVSSKIGVIESLLEKCDILLLGGGMIFTFYKAQGLSVGSSLVE 316

Query: 320 HGVCKEAAELIQFSLDKRVRIVYPKDFWCAN-VDTSKKMEIFASHDIPDGWLPVDLGPTS 379
               + A  L+  +  + V ++ P D   A+        +I  +  IPDGW+ +D+GP S
Sbjct: 317 EDKLELATTLLAKAKARGVSLLLPTDVVIADKFAPDANSKIVPASAIPDGWMGLDIGPDS 376

Query: 380 LDEINALLMNSKKIIWIGPVKFSDSSQSARGASRLARKLYDLSQRDCDITVVGTTACKAI 439
           +   N  L  ++ +IW GP+   +  + A+G   +A KL +LS++     + G  +  A+
Sbjct: 377 VKTFNEALDTTQTVIWNGPMGVFEFEKFAKGTEAVANKLAELSKKGVTTIIGGGDSVAAV 436

Query: 440 MQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRAYPYEI 478
            +        ++        +  +GK LPGV+ALD A P  +
Sbjct: 437 EKVGVAGVMSHISTGGGASLELLEGKVLPGVVALDEATPVTV 478

BLAST of CSPI03G33330 vs. TAIR 10
Match: AT1G56190.2 (Phosphoglycerate kinase family protein )

HSP 1 Score: 184.5 bits (467), Expect = 2.8e-46
Identity = 118/402 (29.35%), Postives = 204/402 (50.75%), Query Frame = 0

Query: 80  QTLGEFPREELAGKVVMVRFDSTLLLLEEMDLKTNS-MKNAVFTIKYLQKSGAKVILASN 139
           +++G+    +L GK V VR D  + L +  ++  ++ ++ A+ TIK+L ++GAKVIL+++
Sbjct: 4   KSVGDLNSVDLKGKKVFVRADLNVPLDDNQNITDDTRIRAAIPTIKFLIENGAKVILSTH 63

Query: 140 -WNTNSAARYLDIKSVVDYLSSVLQLKVCPV-KCSSYSMMLTEEGFEKADILLLENLSVF 199
                       +  +V  LS +L ++V     C    +        +  +LLLEN+  +
Sbjct: 64  LGRPKGVTPKFSLAPLVPRLSELLGIEVVKADDCIGPEVETLVASLPEGGVLLLENVRFY 123

Query: 200 KEEVSNCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCSTCLAGFNFQEGLCQLK 259
           KEE  N   F+K L+   D++VND+F  +HR  AST G+T+F    +AGF  Q+ L  L 
Sbjct: 124 KEEEKNEPDFAKKLASLADLYVNDAFGTAHRAHASTEGVTKFLKPSVAGFLLQKELDYLV 183

Query: 260 KAAQTKRQPYVAIIGGGNLLNKLAALHVLASTCSVLVFVGMMSFQIMQALGLSVSYRLMN 319
            A    ++P+ AI+GG  + +K+  +  L   C +L+  G M F   +A GLSV   L+ 
Sbjct: 184 GAVSNPKRPFAAIVGGSKVSSKIGVIESLLEKCDILLLGGGMIFTFYKAQGLSVGSSLVE 243

Query: 320 HGVCKEAAELIQFSLDKRVRIVYPKDFWCAN-VDTSKKMEIFASHDIPDGWLPVDLGPTS 379
               + A  L+  +  + V ++ P D   A+        +I  +  IPDGW+ +D+GP S
Sbjct: 244 EDKLELATTLLAKAKARGVSLLLPTDVVIADKFAPDANSKIVPASAIPDGWMGLDIGPDS 303

Query: 380 LDEINALLMNSKKIIWIGPVKFSDSSQSARGASRLARKLYDLSQRDCDITVVGTTACKAI 439
           +   N  L  ++ +IW GP+   +  + A+G   +A KL +LS++     + G  +  A+
Sbjct: 304 VKTFNEALDTTQTVIWNGPMGVFEFEKFAKGTEAVANKLAELSKKGVTTIIGGGDSVAAV 363

Query: 440 MQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRAYPYEI 478
            +        ++        +  +GK LPGV+ALD A P  +
Sbjct: 364 EKVGVAGVMSHISTGGGASLELLEGKVLPGVVALDEATPVTV 405

BLAST of CSPI03G33330 vs. TAIR 10
Match: AT1G79550.1 (phosphoglycerate kinase )

HSP 1 Score: 180.3 bits (456), Expect = 5.3e-45
Identity = 119/397 (29.97%), Postives = 199/397 (50.13%), Query Frame = 0

Query: 80  QTLGEFPREELAGKVVMVRFDSTLLLLEEMDLKTNS-MKNAVFTIKYLQKSGAKVILASN 139
           +++G     +L GK V VR D  + L +  ++  ++ ++ AV TIKYL  +G++V+L S+
Sbjct: 5   RSVGTLKEADLKGKSVFVRVDLNVPLDDNSNITDDTRIRAAVPTIKYLMGNGSRVVLCSH 64

Query: 140 -WNTNSAARYLDIKSVVDYLSSVLQLKVCPVKCS-SYSMMLTEEGFEKADILLLENLSVF 199
                       +K +V  LS +L ++V     S    +     G  +  +LLLEN+  +
Sbjct: 65  LGRPKGVTPKYSLKPLVPRLSELLGVEVVMANDSIGEEVQKLVAGLPEGGVLLLENVRFY 124

Query: 200 KEEVSNCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCSTCLAGFNFQEGLCQLK 259
            EE  N  +F+K L+   D++VND+F  +HR  AST G+ +F    +AGF  Q+ L  L 
Sbjct: 125 AEEEKNDPEFAKKLAALADVYVNDAFGTAHRAHASTEGVAKFLKPSVAGFLMQKELDYLV 184

Query: 260 KAAQTKRQPYVAIIGGGNLLNKLAALHVLASTCSVLVFVGMMSFQIMQALGLSVSYRLMN 319
            A    ++P+ AI+GG  +  K+  +  L +T  +L+  G M F   +A GLSV   L+ 
Sbjct: 185 GAVANPKKPFAAIVGGSKVSTKIGVIESLLNTVDILLLGGGMIFTFYKAQGLSVGSSLVE 244

Query: 320 HGVCKEAAELIQFSLDKRVRIVYPKDFWCAN-VDTSKKMEIFASHDIPDGWLPVDLGPTS 379
                 A  L++ +  K V ++ P D   A+        +I  +  IPDGW+ +D+GP S
Sbjct: 245 EDKLDLAKSLMEKAKAKGVSLLLPTDVVIADKFAPDANSKIVPATAIPDGWMGLDIGPDS 304

Query: 380 LDEINALLMNSKKIIWIGPVKFSDSSQSARGASRLARKLYDLSQRDCDITVVGTTACKAI 439
           +   +  L  +K IIW GP+   +  + A G   +A++L +LS +     + G  +  A+
Sbjct: 305 IKTFSEALDTTKTIIWNGPMGVFEFDKFAAGTEAVAKQLAELSGKGVTTIIGGGDSVAAV 364

Query: 440 MQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRA 473
            +        ++        +  +GK LPGVLALD A
Sbjct: 365 EKVGLADKMSHISTGGGASLELLEGKPLPGVLALDEA 401

BLAST of CSPI03G33330 vs. TAIR 10
Match: AT1G79550.2 (phosphoglycerate kinase )

HSP 1 Score: 180.3 bits (456), Expect = 5.3e-45
Identity = 119/397 (29.97%), Postives = 199/397 (50.13%), Query Frame = 0

Query: 80  QTLGEFPREELAGKVVMVRFDSTLLLLEEMDLKTNS-MKNAVFTIKYLQKSGAKVILASN 139
           +++G     +L GK V VR D  + L +  ++  ++ ++ AV TIKYL  +G++V+L S+
Sbjct: 5   RSVGTLKEADLKGKSVFVRVDLNVPLDDNSNITDDTRIRAAVPTIKYLMGNGSRVVLCSH 64

Query: 140 -WNTNSAARYLDIKSVVDYLSSVLQLKVCPVKCS-SYSMMLTEEGFEKADILLLENLSVF 199
                       +K +V  LS +L ++V     S    +     G  +  +LLLEN+  +
Sbjct: 65  LGRPKGVTPKYSLKPLVPRLSELLGVEVVMANDSIGEEVQKLVAGLPEGGVLLLENVRFY 124

Query: 200 KEEVSNCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCSTCLAGFNFQEGLCQLK 259
            EE  N  +F+K L+   D++VND+F  +HR  AST G+ +F    +AGF  Q+ L  L 
Sbjct: 125 AEEEKNDPEFAKKLAALADVYVNDAFGTAHRAHASTEGVAKFLKPSVAGFLMQKELDYLV 184

Query: 260 KAAQTKRQPYVAIIGGGNLLNKLAALHVLASTCSVLVFVGMMSFQIMQALGLSVSYRLMN 319
            A    ++P+ AI+GG  +  K+  +  L +T  +L+  G M F   +A GLSV   L+ 
Sbjct: 185 GAVANPKKPFAAIVGGSKVSTKIGVIESLLNTVDILLLGGGMIFTFYKAQGLSVGSSLVE 244

Query: 320 HGVCKEAAELIQFSLDKRVRIVYPKDFWCAN-VDTSKKMEIFASHDIPDGWLPVDLGPTS 379
                 A  L++ +  K V ++ P D   A+        +I  +  IPDGW+ +D+GP S
Sbjct: 245 EDKLDLAKSLMEKAKAKGVSLLLPTDVVIADKFAPDANSKIVPATAIPDGWMGLDIGPDS 304

Query: 380 LDEINALLMNSKKIIWIGPVKFSDSSQSARGASRLARKLYDLSQRDCDITVVGTTACKAI 439
           +   +  L  +K IIW GP+   +  + A G   +A++L +LS +     + G  +  A+
Sbjct: 305 IKTFSEALDTTKTIIWNGPMGVFEFDKFAAGTEAVAKQLAELSGKGVTTIIGGGDSVAAV 364

Query: 440 MQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRA 473
            +        ++        +  +GK LPGVLALD A
Sbjct: 365 EKVGLADKMSHISTGGGASLELLEGKPLPGVLALDEA 401

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q429611.5e-4731.23Phosphoglycerate kinase, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1[more]
P294091.2e-4629.95Phosphoglycerate kinase, chloroplastic (Fragment) OS=Spinacia oleracea OX=3562 P... [more]
Q9LD572.1e-4629.85Phosphoglycerate kinase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PGK1... [more]
P127831.4e-4531.16Phosphoglycerate kinase, cytosolic OS=Triticum aestivum OX=4565 PE=2 SV=1[more]
P503184.0e-4529.35Phosphoglycerate kinase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At1g... [more]
Match NameE-valueIdentityDescription
A0A0A0LB010.0e+0099.11Phosphoglycerate kinase OS=Cucumis sativus OX=3659 GN=Csa_3G740160 PE=3 SV=1[more]
A0A1S4DUU00.0e+0096.15Phosphoglycerate kinase OS=Cucumis melo OX=3656 GN=LOC103487132 PE=3 SV=1[more]
A0A5D3DNE90.0e+0096.37Phosphoglycerate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A5A7TS090.0e+0092.99Phosphoglycerate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold... [more]
A0A6J1J0L10.0e+0085.54Phosphoglycerate kinase OS=Cucurbita maxima OX=3661 GN=LOC111481591 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_004137833.10.0e+0099.11phosphoglycerate kinase, cytosolic isoform X1 [Cucumis sativus] >KGN58973.1 hypo... [more]
XP_016899748.10.0e+0096.15PREDICTED: phosphoglycerate kinase, cytosolic-like isoform X1 [Cucumis melo] >XP... [more]
TYK25141.10.0e+0096.37phosphoglycerate kinase, cytosolic-like isoform X1 [Cucumis melo var. makuwa][more]
KAA0043999.10.0e+0092.99phosphoglycerate kinase, cytosolic-like isoform X1 [Cucumis melo var. makuwa][more]
XP_038904993.10.0e+0088.59phosphoglycerate kinase, cytosolic-like isoform X1 [Benincasa hispida] >XP_03890... [more]
Match NameE-valueIdentityDescription
AT3G12780.11.5e-4729.85phosphoglycerate kinase 1 [more]
AT1G56190.12.8e-4629.35Phosphoglycerate kinase family protein [more]
AT1G56190.22.8e-4629.35Phosphoglycerate kinase family protein [more]
AT1G79550.15.3e-4529.97phosphoglycerate kinase [more]
AT1G79550.25.3e-4529.97phosphoglycerate kinase [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001576Phosphoglycerate kinasePRINTSPR00477PHGLYCKINASEcoord: 293..318
score: 24.89
coord: 15..37
score: 27.71
coord: 90..105
score: 43.82
coord: 352..369
score: 25.95
coord: 118..140
score: 44.62
coord: 172..191
score: 30.16
coord: 149..171
score: 25.27
IPR001576Phosphoglycerate kinasePFAMPF00162PGKcoord: 16..343
e-value: 4.3E-54
score: 183.9
IPR001576Phosphoglycerate kinasePANTHERPTHR11406PHOSPHOGLYCERATE KINASEcoord: 1..450
IPR003358tRNA (guanine-N-7) methyltransferase, Trmb typePFAMPF02390Methyltransf_4coord: 394..530
e-value: 1.6E-26
score: 92.8
IPR003358tRNA (guanine-N-7) methyltransferase, Trmb typePROSITEPS51625SAM_MT_TRMBcoord: 347..572
score: 44.203499
IPR015824Phosphoglycerate kinase, N-terminalGENE3D3.40.50.1260coord: 1..163
e-value: 1.0E-28
score: 102.0
IPR015824Phosphoglycerate kinase, N-terminalGENE3D3.40.50.1260coord: 165..374
e-value: 2.3E-35
score: 124.0
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 375..579
e-value: 2.6E-51
score: 175.8
NoneNo IPR availablePIRSRPIRSR000724-2PIRSR000724-2coord: 18..216
e-value: 9.2E-14
score: 48.9
NoneNo IPR availablePANTHERPTHR11406:SF13PHOSPHOGLYCERATE KINASEcoord: 1..450
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 396..509
e-value: 1.35644E-5
score: 42.4171
IPR036043Phosphoglycerate kinase superfamilySUPERFAMILY53748Phosphoglycerate kinasecoord: 16..375
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 394..568

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI03G33330.1CSPI03G33330.1mRNA
CSPI03G33330.2CSPI03G33330.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006094 gluconeogenesis
biological_process GO:0006096 glycolytic process
biological_process GO:0106004 tRNA (guanine-N7)-methylation
biological_process GO:0006400 tRNA modification
cellular_component GO:0005829 cytosol
molecular_function GO:0043531 ADP binding
molecular_function GO:0005524 ATP binding
molecular_function GO:0004618 phosphoglycerate kinase activity
molecular_function GO:0008176 tRNA (guanine-N7-)-methyltransferase activity