CSPI03G32390 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI03G32390
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	RING-type E3 ubiquitin transferase
Location	Chr3: 28926920 .. 28927875 (-)
RNA-Seq Expression	CSPI03G32390
Synteny	CSPI03G32390

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CAATGAACAAAAAAAATCCTACAAATCCAAACAAAAACAATAAAACTAAAATCTTGTATGAACCTGAGCTGTTTAATGATAAGAAACCAATTCCACAGCCTTCCTTTCCCCTTCATTTTTCCGCCATGGACGCCACCGCCTCCTCCATGAATCGATCACCGCCGCCGTACCCAACGCCACATCGCACCGTTGATTTCTCTCCAATTGAGTTCATTCTCGGCCTTGTCGCCGTCATCACAATCCCTGCCCTAGTCTACTCCTTCATCTTCGCCATCAAGTGCCCGCCCAATTTCCTCCGCCTCTGGCGGCAAAGATCCACAAGGCTCTCCGGCGCCGGCGCCACCGTTGTGGTCGGAAATCGTACTCCTTCTAGTGACGTTGCTGCTGCCGTTAAATACAACAAAGACGAGCACTGCAAGGAAGTCGGCAACGAGTGTCCAGTGTGCCTGTCGGCGTTCGACGACGGTGAAGAGATCCGGCAACTCGCCGTCTGTAAACACTCTTTTCATGTGGATTGTATTGATATGTGGCTCTATTCTCACCCTAATTGTCCTGTTTGTCGAGCCGCCGTCCCCGTCACTGTTAAGCGGCCGATTCACAACCACGCGCCGCGACGGCCGGCGGCTTCTAGAAGCGACGATTTCCACCAAGGACTGCCGGATGCCGGATATTTAGTTTGAAATTCTTCTTCTTCCATCTCTTTGTTCTTCCGTTTTATGCAAATTCGAACCACAGATCGAAAAATTACGTTCAAATTTCATCTCCATTTCCCTATATTTTGTGGATAATTTGGAATTAGATAGTTTTTATTTCATAGAATTTGGAAAAATTGGAATGATTTTCGATTCTGAAATTCAAATCCCAAAAACTTAATAGGATTCAGGATTAGATCTTTTTCTTTTCTTTTTCCTTTTTGGGTGATGAGAAATTGATTTATGAAATTTCCTTCCCTTTTA

mRNA sequence

CAATGAACAAAAAAAATCCTACAAATCCAAACAAAAACAATAAAACTAAAATCTTGTATGAACCTGAGCTGTTTAATGATAAGAAACCAATTCCACAGCCTTCCTTTCCCCTTCATTTTTCCGCCATGGACGCCACCGCCTCCTCCATGAATCGATCACCGCCGCCGTACCCAACGCCACATCGCACCGTTGATTTCTCTCCAATTGAGTTCATTCTCGGCCTTGTCGCCGTCATCACAATCCCTGCCCTAGTCTACTCCTTCATCTTCGCCATCAAGTGCCCGCCCAATTTCCTCCGCCTCTGGCGGCAAAGATCCACAAGGCTCTCCGGCGCCGGCGCCACCGTTGTGGTCGGAAATCGTACTCCTTCTAGTGACGTTGCTGCTGCCGTTAAATACAACAAAGACGAGCACTGCAAGGAAGTCGGCAACGAGTGTCCAGTGTGCCTGTCGGCGTTCGACGACGGTGAAGAGATCCGGCAACTCGCCGTCTGTAAACACTCTTTTCATGTGGATTGTATTGATATGTGGCTCTATTCTCACCCTAATTGTCCTGTTTGTCGAGCCGCCGTCCCCGTCACTGTTAAGCGGCCGATTCACAACCACGCGCCGCGACGGCCGGCGGCTTCTAGAAGCGACGATTTCCACCAAGGACTGCCGGATGCCGGATATTTAGTTTGAAATTCTTCTTCTTCCATCTCTTTGTTCTTCCGTTTTATGCAAATTCGAACCACAGATCGAAAAATTACGTTCAAATTTCATCTCCATTTCCCTATATTTTGTGGATAATTTGGAATTAGATAGTTTTTATTTCATAGAATTTGGAAAAATTGGAATGATTTTCGATTCTGAAATTCAAATCCCAAAAACTTAATAGGATTCAGGATTAGATCTTTTTCTTTTCTTTTTCCTTTTTGGGTGATGAGAAATTGATTTATGAAATTTCCTTCCCTTTTA

Coding sequence (CDS)

ATGAACAAAAAAAATCCTACAAATCCAAACAAAAACAATAAAACTAAAATCTTGTATGAACCTGAGCTGTTTAATGATAAGAAACCAATTCCACAGCCTTCCTTTCCCCTTCATTTTTCCGCCATGGACGCCACCGCCTCCTCCATGAATCGATCACCGCCGCCGTACCCAACGCCACATCGCACCGTTGATTTCTCTCCAATTGAGTTCATTCTCGGCCTTGTCGCCGTCATCACAATCCCTGCCCTAGTCTACTCCTTCATCTTCGCCATCAAGTGCCCGCCCAATTTCCTCCGCCTCTGGCGGCAAAGATCCACAAGGCTCTCCGGCGCCGGCGCCACCGTTGTGGTCGGAAATCGTACTCCTTCTAGTGACGTTGCTGCTGCCGTTAAATACAACAAAGACGAGCACTGCAAGGAAGTCGGCAACGAGTGTCCAGTGTGCCTGTCGGCGTTCGACGACGGTGAAGAGATCCGGCAACTCGCCGTCTGTAAACACTCTTTTCATGTGGATTGTATTGATATGTGGCTCTATTCTCACCCTAATTGTCCTGTTTGTCGAGCCGCCGTCCCCGTCACTGTTAAGCGGCCGATTCACAACCACGCGCCGCGACGGCCGGCGGCTTCTAGAAGCGACGATTTCCACCAAGGACTGCCGGATGCCGGATATTTAGTTTGA

Protein sequence

MNKKNPTNPNKNNKTKILYEPELFNDKKPIPQPSFPLHFSAMDATASSMNRSPPPYPTPHRTVDFSPIEFILGLVAVITIPALVYSFIFAIKCPPNFLRLWRQRSTRLSGAGATVVVGNRTPSSDVAAAVKYNKDEHCKEVGNECPVCLSAFDDGEEIRQLAVCKHSFHVDCIDMWLYSHPNCPVCRAAVPVTVKRPIHNHAPRRPAASRSDDFHQGLPDAGYLV*

Homology

[Close]

BLAST of CSPI03G32390 vs. ExPASy Swiss-Prot
Match: O80927 (RING-H2 finger protein ATL33 OS=Arabidopsis thaliana OX=3702 GN=ATL33 PE=2 SV=2)

HSP 1 Score: 143.7 bits (361), Expect = 2.6e-33
Identity = 82/227 (36.12%), Postives = 115/227 (50.66%), Query Frame = 0


Query: 30  IPQPSFPLHFSAMDATASSMNRS------PPPYPTPHRTVDFSPIEFILGLVAVITIPAL 89
           +P P+  +  +    T  + N +      PPP+P P R++D +P++ I  ++A + +PAL
Sbjct: 18  VPTPATTVPTTDFPGTTITSNSTFIIIGPPPPFPAPPRSIDLTPLKLIFVVIAFVAVPAL 77

Query: 90  VYSFIFAIKCPPNFLRLWRQRSTRLSGAGATVVVGNRTPSSDV------------AAAVK 149
           VY+  F   C           S R S +  T    + TP + V             +  K
Sbjct: 78  VYALFFNGPC---------SSSRRNSSSSRTSSSSDDTPHATVDTPPITETTVTSESGGK 137

Query: 150 YNKDEHCKEVGNECPVCLSAFDDGEEIRQLAVCKHSFHVDCIDMWLYSHPNCPVCRA--- 209
           ++KD H KE+GNEC VCL  F D +E+RQL+ CKH+FHV CI+ WL  HPNCP+CR    
Sbjct: 138 FHKDTHSKEIGNECSVCLMVFTDSDELRQLSECKHAFHVLCIETWLKDHPNCPICRTDVS 197

Query: 210 --------AVPVTVKRPIHNHAPRR--PAASRSDDFHQGLPDAGYLV 226
                    VPV V   ++     R   A SR DD+ QGLPDA  LV
Sbjct: 198 VKQQTEAPNVPVNVNGNVNRSGGNRRVSATSRDDDWRQGLPDASSLV 235

[Close]

BLAST of CSPI03G32390 vs. ExPASy Swiss-Prot
Match: Q8W571 (RING-H2 finger protein ATL32 OS=Arabidopsis thaliana OX=3702 GN=ATL32 PE=2 SV=3)

HSP 1 Score: 80.5 bits (197), Expect = 2.7e-14
Identity = 50/157 (31.85%), Postives = 73/157 (46.50%), Query Frame = 0


Query: 44  ATASSMNRSPPPYP---TPHRTVDFSPIEFILGLVAVITIPALVYSFIFAIKCPPNFLRL 103
           A A S + SPP      TP +T  F+ +  +  L  +++    VY    A   P +  R 
Sbjct: 26  ANAQSFSPSPPDLQTGHTPSKTTVFAVLVTLFFLTGLLS----VYIRHCARSNPDSSTRY 85

Query: 104 WRQRSTRLSGAGATVVVGNRTPSSDVAAAVKYNKDEHCKEVGNECPVCLSAFDDGEEIRQ 163
           +R R+    G+     + N    S    A    K+        EC +CL+  +D E +R 
Sbjct: 86  FRNRAN--DGSSRRGGLDNAVVESFPVFAYSSVKESKIGSKDLECAICLNELEDHETVRL 145

Query: 164 LAVCKHSFHVDCIDMWLYSHPNCPVCRAAVPVTVKRP 198
           L +C H FH+DCID WLYSH  CPVCR+ +     +P
Sbjct: 146 LPICNHLFHIDCIDTWLYSHATCPVCRSNLTAKSNKP 176

[Close]

BLAST of CSPI03G32390 vs. ExPASy Swiss-Prot
Match: Q9LRB7 (E3 ubiquitin-protein ligase EL5 OS=Oryza sativa subsp. japonica OX=39947 GN=EL5.1 PE=1 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 7.9e-14
Identity = 60/189 (31.75%), Postives = 81/189 (42.86%), Query Frame = 0


Query: 41  AMDATASSMNRSPPPYPTPHRTVDFSPIEFILGLVAVITIPALVYSFIF----------A 100
           AMD ++SS + SP   P     +    I  +  +  +I   AL  +F+           A
Sbjct: 11  AMDESSSSSSPSPVSAPAGQAAMTAGGIATVAAV--LIVFAALTLAFVLLQCYCDERRRA 70

Query: 101 IKCPPNFLRLWRQRSTRLSGAGATVVVGNR-----------TPSSDVAAAVKYNKDEHCK 160
           +       R  R R  R SG+G     G             T  S   AA    ++E   
Sbjct: 71  VTTTSTSGRGRRPRPRRRSGSGGDGGTGGGVDPEVLRSLPVTVYSRSTAAAAAKEEEEED 130

Query: 161 EVGNECPVCLSAFDDGEEIRQLAVCKHSFHVDCIDMWLYSHPNCPVCRAAVPVTVKRPIH 209
           + G EC VCL+  +DGEE R L  C H FH +C+DMWL SH  CP+CR  V V    P+ 
Sbjct: 131 DDGVECAVCLAELEDGEEARFLPRCGHGFHAECVDMWLGSHSTCPLCRLTV-VVPPPPLP 190

[Close]

BLAST of CSPI03G32390 vs. ExPASy Swiss-Prot
Match: Q9LX93 (E3 ubiquitin-protein ligase RING1 OS=Arabidopsis thaliana OX=3702 GN=ATL55 PE=1 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 5.1e-13
Identity = 55/182 (30.22%), Postives = 76/182 (41.76%), Query Frame = 0


Query: 53  PPPYPTPHRTVDFSPIEFILGLVAVITIPALVYSFIFAIKCPPNFLRLWRQRSTRLSGAG 112
           PPP P P       P E  L L   I +   +  F+F +     FL L   +  R+S A 
Sbjct: 27  PPPPPPP------PPRELSLLLPTSICVVGSIILFLFLV----FFLYLHITQQRRISAAS 86

Query: 113 ATVVVGNRTPSSDVAAAVKYNKDEHCKEV--------------------------GNECP 172
            T    N+    D      ++   H  ++                          G EC 
Sbjct: 87  VTPGDTNQQEDEDETEERDFSDFHHVWQIPTVGLHRSAINSITVVGFKKGEGIIDGTECS 146

Query: 173 VCLSAFDDGEEIRQLAVCKHSFHVDCIDMWLYSHPNCPVCRAAVPVTVKRPIH-----NH 204
           VCL+ F++ E +R L  C H+FH++CID WL SH NCP+CRA V + +  P H     NH
Sbjct: 147 VCLNEFEEDESLRLLPKCSHAFHLNCIDTWLLSHKNCPLCRAPV-LLITEPPHQETETNH 197

[Close]

BLAST of CSPI03G32390 vs. ExPASy Swiss-Prot
Match: P0C041 (Putative RING-H2 finger protein ATL53 OS=Arabidopsis thaliana OX=3702 GN=ATL53 PE=3 SV=2)

HSP 1 Score: 73.9 bits (180), Expect = 2.5e-12
Identity = 30/60 (50.00%), Postives = 41/60 (68.33%), Query Frame = 0


Query: 142 GNECPVCLSAFDDGEEIRQLAVCKHSFHVDCIDMWLYSHPNCPVCRAAVPV-TVKRPIHN 201
           G +C +CL  F++ E +R L  C H+FHV CID WL SH NCP+CRA + V T ++P H+
Sbjct: 152 GTDCSICLGEFNEDESLRLLPKCNHTFHVVCIDRWLKSHSNCPLCRAKIIVPTTQQPEHH 211

[Close]

BLAST of CSPI03G32390 vs. ExPASy TrEMBL
Match: A0A0A0LA67 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_3G734330 PE=3 SV=1)

HSP 1 Score: 388.3 bits (996), Expect = 2.2e-104
Identity = 184/184 (100.00%), Postives = 184/184 (100.00%), Query Frame = 0


Query: 42  MDATASSMNRSPPPYPTPHRTVDFSPIEFILGLVAVITIPALVYSFIFAIKCPPNFLRLW 101
           MDATASSMNRSPPPYPTPHRTVDFSPIEFILGLVAVITIPALVYSFIFAIKCPPNFLRLW
Sbjct: 1   MDATASSMNRSPPPYPTPHRTVDFSPIEFILGLVAVITIPALVYSFIFAIKCPPNFLRLW 60

Query: 102 RQRSTRLSGAGATVVVGNRTPSSDVAAAVKYNKDEHCKEVGNECPVCLSAFDDGEEIRQL 161
           RQRSTRLSGAGATVVVGNRTPSSDVAAAVKYNKDEHCKEVGNECPVCLSAFDDGEEIRQL
Sbjct: 61  RQRSTRLSGAGATVVVGNRTPSSDVAAAVKYNKDEHCKEVGNECPVCLSAFDDGEEIRQL 120

Query: 162 AVCKHSFHVDCIDMWLYSHPNCPVCRAAVPVTVKRPIHNHAPRRPAASRSDDFHQGLPDA 221
           AVCKHSFHVDCIDMWLYSHPNCPVCRAAVPVTVKRPIHNHAPRRPAASRSDDFHQGLPDA
Sbjct: 121 AVCKHSFHVDCIDMWLYSHPNCPVCRAAVPVTVKRPIHNHAPRRPAASRSDDFHQGLPDA 180

Query: 222 GYLV 226
           GYLV
Sbjct: 181 GYLV 184

[Close]

BLAST of CSPI03G32390 vs. ExPASy TrEMBL
Match: A0A5D3DN65 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G001760 PE=3 SV=1)

HSP 1 Score: 372.1 bits (954), Expect = 1.7e-99
Identity = 175/184 (95.11%), Postives = 177/184 (96.20%), Query Frame = 0


Query: 42  MDATASSMNRSPPPYPTPHRTVDFSPIEFILGLVAVITIPALVYSFIFAIKCPPNFLRLW 101
           MD TASSMNRSPPPYPTPHRTVDFSPIEFILGLVAVITIPAL YSFIFAIKCPPNFLRLW
Sbjct: 1   MDTTASSMNRSPPPYPTPHRTVDFSPIEFILGLVAVITIPALAYSFIFAIKCPPNFLRLW 60

Query: 102 RQRSTRLSGAGATVVVGNRTPSSDVAAAVKYNKDEHCKEVGNECPVCLSAFDDGEEIRQL 161
           RQRSTRLS AG T+VVGNRTPSSDVAAAVKYNKDEHCKEVG ECPVCLSAFDDGEEIRQL
Sbjct: 61  RQRSTRLSAAGVTMVVGNRTPSSDVAAAVKYNKDEHCKEVGTECPVCLSAFDDGEEIRQL 120

Query: 162 AVCKHSFHVDCIDMWLYSHPNCPVCRAAVPVTVKRPIHNHAPRRPAASRSDDFHQGLPDA 221
             CKHSFHVDCIDMWLYSHPNCPVCRAAVPVTVKRPI+NHAPRRPAASRSDDFHQGLPDA
Sbjct: 121 TTCKHSFHVDCIDMWLYSHPNCPVCRAAVPVTVKRPINNHAPRRPAASRSDDFHQGLPDA 180

Query: 222 GYLV 226
           GYLV
Sbjct: 181 GYLV 184

[Close]

BLAST of CSPI03G32390 vs. ExPASy TrEMBL
Match: A0A1S3B6Q9 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103486397 PE=3 SV=1)

HSP 1 Score: 372.1 bits (954), Expect = 1.7e-99
Identity = 175/184 (95.11%), Postives = 177/184 (96.20%), Query Frame = 0


Query: 42  MDATASSMNRSPPPYPTPHRTVDFSPIEFILGLVAVITIPALVYSFIFAIKCPPNFLRLW 101
           MD TASSMNRSPPPYPTPHRTVDFSPIEFILGLVAVITIPAL YSFIFAIKCPPNFLRLW
Sbjct: 1   MDTTASSMNRSPPPYPTPHRTVDFSPIEFILGLVAVITIPALAYSFIFAIKCPPNFLRLW 60

Query: 102 RQRSTRLSGAGATVVVGNRTPSSDVAAAVKYNKDEHCKEVGNECPVCLSAFDDGEEIRQL 161
           RQRSTRLS AG T+VVGNRTPSSDVAAAVKYNKDEHCKEVG ECPVCLSAFDDGEEIRQL
Sbjct: 61  RQRSTRLSAAGVTMVVGNRTPSSDVAAAVKYNKDEHCKEVGTECPVCLSAFDDGEEIRQL 120

Query: 162 AVCKHSFHVDCIDMWLYSHPNCPVCRAAVPVTVKRPIHNHAPRRPAASRSDDFHQGLPDA 221
             CKHSFHVDCIDMWLYSHPNCPVCRAAVPVTVKRPI+NHAPRRPAASRSDDFHQGLPDA
Sbjct: 121 TTCKHSFHVDCIDMWLYSHPNCPVCRAAVPVTVKRPINNHAPRRPAASRSDDFHQGLPDA 180

Query: 222 GYLV 226
           GYLV
Sbjct: 181 GYLV 184

[Close]

BLAST of CSPI03G32390 vs. ExPASy TrEMBL
Match: A0A6J1F238 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111441690 PE=3 SV=1)

HSP 1 Score: 310.8 bits (795), Expect = 4.5e-81
Identity = 148/186 (79.57%), Postives = 157/186 (84.41%), Query Frame = 0


Query: 40  SAMDATASSMNRSPPPYPTPHRTVDFSPIEFILGLVAVITIPALVYSFIFAIKCPPNFLR 99
           S MD +A+ MNRS PP+PTPHRT DFSPIEFILGL+AVI IPALVYSF FAIKCPP  LR
Sbjct: 2   SQMDTSAALMNRSTPPHPTPHRTNDFSPIEFILGLIAVIAIPALVYSFFFAIKCPPGLLR 61

Query: 100 LWRQRSTRLSGAGATVVVGNRTPSSDVAAAVKYNKDEHCKEVGNECPVCLSAFDDGEEIR 159
           LWR+RS RLSGA  TVV GNR+  SDVA   KYNKDE  KEVGNECPVCLSAF DGEEIR
Sbjct: 62  LWRRRSARLSGAPVTVVAGNRSSYSDVAVTFKYNKDEVGKEVGNECPVCLSAFVDGEEIR 121

Query: 160 QLAVCKHSFHVDCIDMWLYSHPNCPVCRAAVPVTVKRPIHNHAPRRPAASRSDDFHQGLP 219
           QL  CKHSFHVDCIDMW YSHPNCPVCRA + + VKRPI+N APRRPAASRSDDFHQGLP
Sbjct: 122 QLIACKHSFHVDCIDMWFYSHPNCPVCRAEIAIAVKRPINNRAPRRPAASRSDDFHQGLP 181

Query: 220 DAGYLV 226
           DAG LV
Sbjct: 182 DAGNLV 187

[Close]

BLAST of CSPI03G32390 vs. ExPASy TrEMBL
Match: A0A6J1J6T6 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111481888 PE=3 SV=1)

HSP 1 Score: 303.9 bits (777), Expect = 5.6e-79
Identity = 146/186 (78.49%), Postives = 155/186 (83.33%), Query Frame = 0


Query: 40  SAMDATASSMNRSPPPYPTPHRTVDFSPIEFILGLVAVITIPALVYSFIFAIKCPPNFLR 99
           S MD++A+ MNRS PP+P  HRT DFSP EFILGLVAVI IPALVYSF FAIKCPP  LR
Sbjct: 2   SQMDSSAALMNRSTPPHPMTHRTNDFSPFEFILGLVAVIAIPALVYSFFFAIKCPPGLLR 61

Query: 100 LWRQRSTRLSGAGATVVVGNRTPSSDVAAAVKYNKDEHCKEVGNECPVCLSAFDDGEEIR 159
           LWR+RS RLSGA   VV GNR+  SDVAA  KYNKDE  KEVGNECPVCLSAF DGEEIR
Sbjct: 62  LWRRRSARLSGAPVAVVAGNRSSYSDVAATFKYNKDEVGKEVGNECPVCLSAFVDGEEIR 121

Query: 160 QLAVCKHSFHVDCIDMWLYSHPNCPVCRAAVPVTVKRPIHNHAPRRPAASRSDDFHQGLP 219
           QL  CKHSFHVDCIDMW YSHPNCPVCRA + + VKRPI+N APRRPAASRSDDFHQGLP
Sbjct: 122 QLIACKHSFHVDCIDMWFYSHPNCPVCRAEIVIAVKRPINNRAPRRPAASRSDDFHQGLP 181

Query: 220 DAGYLV 226
           DAG LV
Sbjct: 182 DAGNLV 187

[Close]

BLAST of CSPI03G32390 vs. NCBI nr
Match: KAE8651013.1 (hypothetical protein Csa_001104 [Cucumis sativus])

HSP 1 Score: 470.7 bits (1210), Expect = 7.1e-129
Identity = 225/225 (100.00%), Postives = 225/225 (100.00%), Query Frame = 0


Query: 1   MNKKNPTNPNKNNKTKILYEPELFNDKKPIPQPSFPLHFSAMDATASSMNRSPPPYPTPH 60
           MNKKNPTNPNKNNKTKILYEPELFNDKKPIPQPSFPLHFSAMDATASSMNRSPPPYPTPH
Sbjct: 1   MNKKNPTNPNKNNKTKILYEPELFNDKKPIPQPSFPLHFSAMDATASSMNRSPPPYPTPH 60

Query: 61  RTVDFSPIEFILGLVAVITIPALVYSFIFAIKCPPNFLRLWRQRSTRLSGAGATVVVGNR 120
           RTVDFSPIEFILGLVAVITIPALVYSFIFAIKCPPNFLRLWRQRSTRLSGAGATVVVGNR
Sbjct: 61  RTVDFSPIEFILGLVAVITIPALVYSFIFAIKCPPNFLRLWRQRSTRLSGAGATVVVGNR 120

Query: 121 TPSSDVAAAVKYNKDEHCKEVGNECPVCLSAFDDGEEIRQLAVCKHSFHVDCIDMWLYSH 180
           TPSSDVAAAVKYNKDEHCKEVGNECPVCLSAFDDGEEIRQLAVCKHSFHVDCIDMWLYSH
Sbjct: 121 TPSSDVAAAVKYNKDEHCKEVGNECPVCLSAFDDGEEIRQLAVCKHSFHVDCIDMWLYSH 180

Query: 181 PNCPVCRAAVPVTVKRPIHNHAPRRPAASRSDDFHQGLPDAGYLV 226
           PNCPVCRAAVPVTVKRPIHNHAPRRPAASRSDDFHQGLPDAGYLV
Sbjct: 181 PNCPVCRAAVPVTVKRPIHNHAPRRPAASRSDDFHQGLPDAGYLV 225

[Close]

BLAST of CSPI03G32390 vs. NCBI nr
Match: XP_004137943.1 (RING-H2 finger protein ATL33 [Cucumis sativus])

HSP 1 Score: 388.3 bits (996), Expect = 4.6e-104
Identity = 184/184 (100.00%), Postives = 184/184 (100.00%), Query Frame = 0


Query: 42  MDATASSMNRSPPPYPTPHRTVDFSPIEFILGLVAVITIPALVYSFIFAIKCPPNFLRLW 101
           MDATASSMNRSPPPYPTPHRTVDFSPIEFILGLVAVITIPALVYSFIFAIKCPPNFLRLW
Sbjct: 1   MDATASSMNRSPPPYPTPHRTVDFSPIEFILGLVAVITIPALVYSFIFAIKCPPNFLRLW 60

Query: 102 RQRSTRLSGAGATVVVGNRTPSSDVAAAVKYNKDEHCKEVGNECPVCLSAFDDGEEIRQL 161
           RQRSTRLSGAGATVVVGNRTPSSDVAAAVKYNKDEHCKEVGNECPVCLSAFDDGEEIRQL
Sbjct: 61  RQRSTRLSGAGATVVVGNRTPSSDVAAAVKYNKDEHCKEVGNECPVCLSAFDDGEEIRQL 120

Query: 162 AVCKHSFHVDCIDMWLYSHPNCPVCRAAVPVTVKRPIHNHAPRRPAASRSDDFHQGLPDA 221
           AVCKHSFHVDCIDMWLYSHPNCPVCRAAVPVTVKRPIHNHAPRRPAASRSDDFHQGLPDA
Sbjct: 121 AVCKHSFHVDCIDMWLYSHPNCPVCRAAVPVTVKRPIHNHAPRRPAASRSDDFHQGLPDA 180

Query: 222 GYLV 226
           GYLV
Sbjct: 181 GYLV 184

[Close]

BLAST of CSPI03G32390 vs. NCBI nr
Match: XP_008442561.1 (PREDICTED: RING-H2 finger protein ATL33 [Cucumis melo] >KAA0044082.1 RING-H2 finger protein ATL33 [Cucumis melo var. makuwa] >TYK25055.1 RING-H2 finger protein ATL33 [Cucumis melo var. makuwa])

HSP 1 Score: 372.1 bits (954), Expect = 3.4e-99
Identity = 175/184 (95.11%), Postives = 177/184 (96.20%), Query Frame = 0


Query: 42  MDATASSMNRSPPPYPTPHRTVDFSPIEFILGLVAVITIPALVYSFIFAIKCPPNFLRLW 101
           MD TASSMNRSPPPYPTPHRTVDFSPIEFILGLVAVITIPAL YSFIFAIKCPPNFLRLW
Sbjct: 1   MDTTASSMNRSPPPYPTPHRTVDFSPIEFILGLVAVITIPALAYSFIFAIKCPPNFLRLW 60

Query: 102 RQRSTRLSGAGATVVVGNRTPSSDVAAAVKYNKDEHCKEVGNECPVCLSAFDDGEEIRQL 161
           RQRSTRLS AG T+VVGNRTPSSDVAAAVKYNKDEHCKEVG ECPVCLSAFDDGEEIRQL
Sbjct: 61  RQRSTRLSAAGVTMVVGNRTPSSDVAAAVKYNKDEHCKEVGTECPVCLSAFDDGEEIRQL 120

Query: 162 AVCKHSFHVDCIDMWLYSHPNCPVCRAAVPVTVKRPIHNHAPRRPAASRSDDFHQGLPDA 221
             CKHSFHVDCIDMWLYSHPNCPVCRAAVPVTVKRPI+NHAPRRPAASRSDDFHQGLPDA
Sbjct: 121 TTCKHSFHVDCIDMWLYSHPNCPVCRAAVPVTVKRPINNHAPRRPAASRSDDFHQGLPDA 180

Query: 222 GYLV 226
           GYLV
Sbjct: 181 GYLV 184

[Close]

BLAST of CSPI03G32390 vs. NCBI nr
Match: XP_038905078.1 (RING-H2 finger protein ATL33 [Benincasa hispida])

HSP 1 Score: 351.3 bits (900), Expect = 6.3e-93
Identity = 167/184 (90.76%), Postives = 169/184 (91.85%), Query Frame = 0


Query: 42  MDATASSMNRSPPPYPTPHRTVDFSPIEFILGLVAVITIPALVYSFIFAIKCPPNFLRLW 101
           MD +AS MNRSPPP PTPHRTVDFSPIEFILGLVAVITIPALVYSF FAIKCPPN LRLW
Sbjct: 1   MDKSASLMNRSPPPSPTPHRTVDFSPIEFILGLVAVITIPALVYSFFFAIKCPPNLLRLW 60

Query: 102 RQRSTRLSGAGATVVVGNRTPSSDVAAAVKYNKDEHCKEVGNECPVCLSAFDDGEEIRQL 161
           R RS RLSGA  TVVV NRTPSSDVAAAVKYNKDEHCKEV NECPVCLSAFDDGEEIRQL
Sbjct: 61  RHRSARLSGAAVTVVVRNRTPSSDVAAAVKYNKDEHCKEVSNECPVCLSAFDDGEEIRQL 120

Query: 162 AVCKHSFHVDCIDMWLYSHPNCPVCRAAVPVTVKRPIHNHAPRRPAASRSDDFHQGLPDA 221
           A CKHSFHVDCIDMWLYSHPNCPVCRAAVPV VKRPI+NHAPRRPA SRSDDF QGLPDA
Sbjct: 121 AACKHSFHVDCIDMWLYSHPNCPVCRAAVPVAVKRPINNHAPRRPAVSRSDDFQQGLPDA 180

Query: 222 GYLV 226
           GYLV
Sbjct: 181 GYLV 184

[Close]

BLAST of CSPI03G32390 vs. NCBI nr
Match: XP_022934546.1 (RING-H2 finger protein ATL33-like [Cucurbita moschata] >KAG6580552.1 RING-H2 finger protein ATL33, partial [Cucurbita argyrosperma subsp. sororia] >KAG7017306.1 RING-H2 finger protein ATL33, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 310.8 bits (795), Expect = 9.4e-81
Identity = 148/186 (79.57%), Postives = 157/186 (84.41%), Query Frame = 0


Query: 40  SAMDATASSMNRSPPPYPTPHRTVDFSPIEFILGLVAVITIPALVYSFIFAIKCPPNFLR 99
           S MD +A+ MNRS PP+PTPHRT DFSPIEFILGL+AVI IPALVYSF FAIKCPP  LR
Sbjct: 2   SQMDTSAALMNRSTPPHPTPHRTNDFSPIEFILGLIAVIAIPALVYSFFFAIKCPPGLLR 61

Query: 100 LWRQRSTRLSGAGATVVVGNRTPSSDVAAAVKYNKDEHCKEVGNECPVCLSAFDDGEEIR 159
           LWR+RS RLSGA  TVV GNR+  SDVA   KYNKDE  KEVGNECPVCLSAF DGEEIR
Sbjct: 62  LWRRRSARLSGAPVTVVAGNRSSYSDVAVTFKYNKDEVGKEVGNECPVCLSAFVDGEEIR 121

Query: 160 QLAVCKHSFHVDCIDMWLYSHPNCPVCRAAVPVTVKRPIHNHAPRRPAASRSDDFHQGLP 219
           QL  CKHSFHVDCIDMW YSHPNCPVCRA + + VKRPI+N APRRPAASRSDDFHQGLP
Sbjct: 122 QLIACKHSFHVDCIDMWFYSHPNCPVCRAEIAIAVKRPINNRAPRRPAASRSDDFHQGLP 181

Query: 220 DAGYLV 226
           DAG LV
Sbjct: 182 DAGNLV 187

[Close]

BLAST of CSPI03G32390 vs. TAIR 10
Match: AT2G37580.1 (RING/U-box superfamily protein )

HSP 1 Score: 143.7 bits (361), Expect = 1.9e-34
Identity = 82/227 (36.12%), Postives = 115/227 (50.66%), Query Frame = 0


Query: 30  IPQPSFPLHFSAMDATASSMNRS------PPPYPTPHRTVDFSPIEFILGLVAVITIPAL 89
           +P P+  +  +    T  + N +      PPP+P P R++D +P++ I  ++A + +PAL
Sbjct: 18  VPTPATTVPTTDFPGTTITSNSTFIIIGPPPPFPAPPRSIDLTPLKLIFVVIAFVAVPAL 77

Query: 90  VYSFIFAIKCPPNFLRLWRQRSTRLSGAGATVVVGNRTPSSDV------------AAAVK 149
           VY+  F   C           S R S +  T    + TP + V             +  K
Sbjct: 78  VYALFFNGPC---------SSSRRNSSSSRTSSSSDDTPHATVDTPPITETTVTSESGGK 137

Query: 150 YNKDEHCKEVGNECPVCLSAFDDGEEIRQLAVCKHSFHVDCIDMWLYSHPNCPVCRA--- 209
           ++KD H KE+GNEC VCL  F D +E+RQL+ CKH+FHV CI+ WL  HPNCP+CR    
Sbjct: 138 FHKDTHSKEIGNECSVCLMVFTDSDELRQLSECKHAFHVLCIETWLKDHPNCPICRTDVS 197

Query: 210 --------AVPVTVKRPIHNHAPRR--PAASRSDDFHQGLPDAGYLV 226
                    VPV V   ++     R   A SR DD+ QGLPDA  LV
Sbjct: 198 VKQQTEAPNVPVNVNGNVNRSGGNRRVSATSRDDDWRQGLPDASSLV 235

[Close]

BLAST of CSPI03G32390 vs. TAIR 10
Match: AT4G40070.1 (RING/U-box superfamily protein )

HSP 1 Score: 80.5 bits (197), Expect = 1.9e-15
Identity = 50/157 (31.85%), Postives = 73/157 (46.50%), Query Frame = 0


Query: 44  ATASSMNRSPPPYP---TPHRTVDFSPIEFILGLVAVITIPALVYSFIFAIKCPPNFLRL 103
           A A S + SPP      TP +T  F+ +  +  L  +++    VY    A   P +  R 
Sbjct: 26  ANAQSFSPSPPDLQTGHTPSKTTVFAVLVTLFFLTGLLS----VYIRHCARSNPDSSTRY 85

Query: 104 WRQRSTRLSGAGATVVVGNRTPSSDVAAAVKYNKDEHCKEVGNECPVCLSAFDDGEEIRQ 163
           +R R+    G+     + N    S    A    K+        EC +CL+  +D E +R 
Sbjct: 86  FRNRAN--DGSSRRGGLDNAVVESFPVFAYSSVKESKIGSKDLECAICLNELEDHETVRL 145

Query: 164 LAVCKHSFHVDCIDMWLYSHPNCPVCRAAVPVTVKRP 198
           L +C H FH+DCID WLYSH  CPVCR+ +     +P
Sbjct: 146 LPICNHLFHIDCIDTWLYSHATCPVCRSNLTAKSNKP 176

[Close]

BLAST of CSPI03G32390 vs. TAIR 10
Match: AT5G10380.1 (RING/U-box superfamily protein )

HSP 1 Score: 76.3 bits (186), Expect = 3.6e-14
Identity = 55/182 (30.22%), Postives = 76/182 (41.76%), Query Frame = 0


Query: 53  PPPYPTPHRTVDFSPIEFILGLVAVITIPALVYSFIFAIKCPPNFLRLWRQRSTRLSGAG 112
           PPP P P       P E  L L   I +   +  F+F +     FL L   +  R+S A 
Sbjct: 27  PPPPPPP------PPRELSLLLPTSICVVGSIILFLFLV----FFLYLHITQQRRISAAS 86

Query: 113 ATVVVGNRTPSSDVAAAVKYNKDEHCKEV--------------------------GNECP 172
            T    N+    D      ++   H  ++                          G EC 
Sbjct: 87  VTPGDTNQQEDEDETEERDFSDFHHVWQIPTVGLHRSAINSITVVGFKKGEGIIDGTECS 146

Query: 173 VCLSAFDDGEEIRQLAVCKHSFHVDCIDMWLYSHPNCPVCRAAVPVTVKRPIH-----NH 204
           VCL+ F++ E +R L  C H+FH++CID WL SH NCP+CRA V + +  P H     NH
Sbjct: 147 VCLNEFEEDESLRLLPKCSHAFHLNCIDTWLLSHKNCPLCRAPV-LLITEPPHQETETNH 197

[Close]

BLAST of CSPI03G32390 vs. TAIR 10
Match: AT4G17905.1 (RING/U-box superfamily protein )

HSP 1 Score: 73.9 bits (180), Expect = 1.8e-13
Identity = 30/60 (50.00%), Postives = 41/60 (68.33%), Query Frame = 0


Query: 142 GNECPVCLSAFDDGEEIRQLAVCKHSFHVDCIDMWLYSHPNCPVCRAAVPV-TVKRPIHN 201
           G +C +CL  F++ E +R L  C H+FHV CID WL SH NCP+CRA + V T ++P H+
Sbjct: 152 GTDCSICLGEFNEDESLRLLPKCNHTFHVVCIDRWLKSHSNCPLCRAKIIVPTTQQPEHH 211

[Close]

BLAST of CSPI03G32390 vs. TAIR 10
Match: AT1G72220.1 (RING/U-box superfamily protein )

HSP 1 Score: 73.6 bits (179), Expect = 2.4e-13
Identity = 26/47 (55.32%), Postives = 35/47 (74.47%), Query Frame = 0


Query: 144 ECPVCLSAFDDGEEIRQLAVCKHSFHVDCIDMWLYSHPNCPVCRAAV 191
           +CPVCL+ F++ E +R L  C H+FH+ CID WL SH NCP+CRA +
Sbjct: 176 DCPVCLNEFEEDESLRLLPKCNHAFHISCIDTWLSSHTNCPLCRAGI 222

The following BLAST results are available for this feature:

BLAST of CSPI03G32390 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O80927	2.6e-33	36.12	RING-H2 finger protein ATL33 OS=Arabidopsis thaliana OX=3702 GN=ATL33 PE=2 SV=2	[more]
Q8W571	2.7e-14	31.85	RING-H2 finger protein ATL32 OS=Arabidopsis thaliana OX=3702 GN=ATL32 PE=2 SV=3	[more]
Q9LRB7	7.9e-14	31.75	E3 ubiquitin-protein ligase EL5 OS=Oryza sativa subsp. japonica OX=39947 GN=EL5....	[more]
Q9LX93	5.1e-13	30.22	E3 ubiquitin-protein ligase RING1 OS=Arabidopsis thaliana OX=3702 GN=ATL55 PE=1 ...	[more]
P0C041	2.5e-12	50.00	Putative RING-H2 finger protein ATL53 OS=Arabidopsis thaliana OX=3702 GN=ATL53 P...	[more]

BLAST of CSPI03G32390 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LA67	2.2e-104	100.00	RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_3G734330 PE...	[more]
A0A5D3DN65	1.7e-99	95.11	RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A1S3B6Q9	1.7e-99	95.11	RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103486397 PE=3 ...	[more]
A0A6J1F238	4.5e-81	79.57	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111441690...	[more]
A0A6J1J6T6	5.6e-79	78.49	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111481888 P...	[more]

BLAST of CSPI03G32390 vs. NCBI nr


Match Name	E-value	Identity	Description
KAE8651013.1	7.1e-129	100.00	hypothetical protein Csa_001104 [Cucumis sativus]	[more]
XP_004137943.1	4.6e-104	100.00	RING-H2 finger protein ATL33 [Cucumis sativus]	[more]
XP_008442561.1	3.4e-99	95.11	PREDICTED: RING-H2 finger protein ATL33 [Cucumis melo] >KAA0044082.1 RING-H2 fin...	[more]
XP_038905078.1	6.3e-93	90.76	RING-H2 finger protein ATL33 [Benincasa hispida]	[more]
XP_022934546.1	9.4e-81	79.57	RING-H2 finger protein ATL33-like [Cucurbita moschata] >KAG6580552.1 RING-H2 fin...	[more]

BLAST of CSPI03G32390 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G37580.1	1.9e-34	36.12	RING/U-box superfamily protein	[more]
AT4G40070.1	1.9e-15	31.85	RING/U-box superfamily protein	[more]
AT5G10380.1	3.6e-14	30.22	RING/U-box superfamily protein	[more]
AT4G17905.1	1.8e-13	50.00	RING/U-box superfamily protein	[more]
AT1G72220.1	2.4e-13	55.32	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 145..186 e-value: 7.3E-6 score: 35.5
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 143..187 e-value: 1.3E-13 score: 51.0
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 145..187 score: 12.135206
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 118..193 e-value: 4.2E-20 score: 73.2
IPR044675	E3 ubiquitin-protein ligase RING1-like	PANTHER	PTHR45676	RING-H2 FINGER PROTEIN ATL51-RELATED	coord: 45..225
None	No IPR available	PANTHER	PTHR45676:SF88	RING-H2 FINGER PROTEIN ATL33	coord: 45..225
None	No IPR available	CDD	cd16461	RING-H2_EL5_like	coord: 144..187 e-value: 5.31358E-16 score: 67.3351
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 144..193

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G32390.1	CSPI03G32390.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0016021	integral component of membrane