CSPI03G32250 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI03G32250
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Haloacid dehalogenase-like hydrolase (HAD) superfamily protein
Location	Chr3: 28835733 .. 28838462 (+)
RNA-Seq Expression	CSPI03G32250
Synteny	CSPI03G32250

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAATATGAGTCCAGAAAGTACAAGGAACCGGACTCCTCCCTTGTTATTGTTTACGTTATGCCTTTCTATTCAATCTTTCCCCAATTCCCACCACTTTCTTTTGATCATCTTTTAATCAAACGTGATTAGCATTACACTTCAAAGATTAAAAACTATTTCCTTTTCTTTTTCTTTTTCTTGTTCAATTCAACAATGAACGAACCATGCCAATCCTTTTGCCTATATAAACCCCTTTAACCCTCCCTTTCTCTTCCCCCACTCGCTCCATTCTTCATTGTTTCTTAAGGAGAAGAGCAACAACCTTTTCTTTCTTTGGCCCTCTTTCTGTAATAACCTTCTTCTCTTGTTCCCTCATTGTTCTTTTGCTCTTGCCATTTCGTTCCTAGTTGGTAAGTCCAGTTTCTTCTCATGTGGTTCCCTGATTTTGCCTTCTTTTCTCCATTATCTTCCTTGTTTGATCTCTGATTTGGATTTCCTTTCTGGGTTTTGTTGGGTTTTCTATTCTTGCATTTTTTTTTTTTTAAAGTTTTTAATGGGTATCTAAAATGGGGTTTGGATTGAACTTGTAATTTGACTGTTTGATAATACTGAGTAACATAAACATGGGTGTGGTTTTCTTTGTTGGAATTCCTTATGGAATATTGAACTTATGAATGAGATTGGTGTGGACTGAACTTTTTAACAGACTATTTTGTACTGCATTCTTGAATGAACCATTAAAATTGAAATTTTGAAGAGAGAGTATGTTGTTCAAGCATACTGAGATCAGTTGTGATGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGATTTAGTTTGTAGGATTTCTAAATCAAAATGGAATACGAGAACCGGTTCAGGCAGGCTCAGAGACCAAAATATGATTGTCTTCTTTTTGGTAAGAACTTTGTCTTTGAACAATCAGATTCTTTTCTCATATGATTTTTTATTTCTTTCATTTGGAATATAATTTGGTTCGTTTCTAATGTGTGTGGCTGAAACTGTAGATTTAGATGACACTCTGTATCCCTTGAGTTCTGGCATTGCAGCTTCGTGCCTTCAGAATATCAAAGGTAGGATTATAAATATAAAATAAAGCCTGAAATTTCCATTGTGTTGCGATCATCAGTTTCTTGATGTTGTTTGTTACTTCCCTTTTTGTAGATTACATGGTTGAAAAACTGGGGATAGAGCAGAGTAAAATCCCTGACCTGTGCAATTTACTGTACAAGAACTATGGAACAACCATGGCAGGTCTCAGGGTATAGCTTCTTGAACTAATTACTTGTTTCAAAAATGCTTTGAAGAAGTGTAGAACAATAATAACTAATGGGGTTTTTTTTTAATAGGCAATTGGCTATGATTTTGATTATGATGAGTACCACAAGTTTGTTCATGGAAGACTTCCTTATGATAATCTAAAGCCCGATCCTGTTCTAAGGAATCTTCTGTTGAACCTGCCTTATAGGAAAGTTGTAAGTATTTGTGTCTTCTTTTTCAGATTCTGTAGAAACTTAAGATTCATTTTGCTGATTTTTTTCTCTTTTTGTGTGTGTATAGATCTTTACAAATGCTGACAAGATCCATGCAGTTAAAGTTCTTAAAAAACTTGGATTAGAAGACTGTTTTCAAGGCATTATCTGCTTTGAAACCCTGAATCCAACCAACAAGAATTTTGTTTCAGTCGATAAAGATGAAATGAATCCAATCCCATCTGACTCGAAAATTTTTGACATCATTGGCCACTTTCTTCATCCCAATCCTGGGACGGAATTACCAAAAACTCCCATCATTTGCAAACCATCAGAGGCTGCCATTGAAAGAGCTCTCAAAATTGCAGGCCTCAACCCTCAGAGAACTGTAAGAGAAAGAGAACCATCTTATTCTTCACTTATAGCTCAATTTACAGTCCATTCCCTTTCAACTTCATTTTTGGTTTTAACTTGTTCCTTTTGCTGTTTTCAGCTGTTCTTTGAAGATAGTGTTCGAAACATACAAGCTGGGAAACGTGTTGGGCTCGACACCGTCTTGGTAAGACATTTTGAAACCATGTAGATGAGAAAACAAAGGGCAGTTCCTGCTGAAAATTTGAGATATTGTTGGCTAATTTGTTTGTCTTTTTTGTTGTTTGGTACTATAGATTGGCACTTCACAGAGAGTTAAAGGAGCAGATTATGCCTTGGAAAGCATCCACAATCTAAGGGAAGGAATACCTGAGCTTTGGAATGTTGAAATCAAATCAGAAATGGGTTATGCTGGCAAAGTTGGAGTTGAAACTTCAGTGACAGCTTAGAACTACAACATCCTTGTTAGGTTCTTCAAATCCTTCCCCCAAAAAATAATGAAAGAAAAACACAAGAAAAGGGGAAATCTTCCGCCTCCAGTTTCACTGGGCAGCTGTTGTAAATAAGCATTTTGATTATGAGAGTAAAGTTCATAAGTGTAATCTTTTCTGTTTTTCTACAATCACTTAATTAACAAAATCATTGAAAAAAATCACTAAAAAAGATGTTGCCATCTATATTAGATGAATAAAACACAGAGTTCTCATACTCTCCACTAATTTTCTATCCATTCAAATTCATTGCCACCATGTTTTTGCATGGCTGTAGTTCACATGTATCATGTTATGACAACTCCATCCATTCTCTTTACACCTAAATCACATCACGTACTGTTTTATTATCGTAAAATGCATTCTAGACTCAACTCAATATAATTGACAAAAC

mRNA sequence

AAATATGAGTCCAGAAAGTACAAGGAACCGGACTCCTCCCTTGTTATTGTTTACGTTATGCCTTTCTATTCAATCTTTCCCCAATTCCCACCACTTTCTTTTGATCATCTTTTAATCAAACGTGATTAGCATTACACTTCAAAGATTAAAAACTATTTCCTTTTCTTTTTCTTTTTCTTGTTCAATTCAACAATGAACGAACCATGCCAATCCTTTTGCCTATATAAACCCCTTTAACCCTCCCTTTCTCTTCCCCCACTCGCTCCATTCTTCATTGTTTCTTAAGGAGAAGAGCAACAACCTTTTCTTTCTTTGGCCCTCTTTCTGTAATAACCTTCTTCTCTTGTTCCCTCATTGTTCTTTTGCTCTTGCCATTTCGTTCCTAGTTGGATTTCTAAATCAAAATGGAATACGAGAACCGGTTCAGGCAGGCTCAGAGACCAAAATATGATTGTCTTCTTTTTGATTTAGATGACACTCTGTATCCCTTGAGTTCTGGCATTGCAGCTTCGTGCCTTCAGAATATCAAAGATTACATGGTTGAAAAACTGGGGATAGAGCAGAGTAAAATCCCTGACCTGTGCAATTTACTGTACAAGAACTATGGAACAACCATGGCAGGTCTCAGGGCAATTGGCTATGATTTTGATTATGATGAGTACCACAAGTTTGTTCATGGAAGACTTCCTTATGATAATCTAAAGCCCGATCCTGTTCTAAGGAATCTTCTGTTGAACCTGCCTTATAGGAAAGTTATCTTTACAAATGCTGACAAGATCCATGCAGTTAAAGTTCTTAAAAAACTTGGATTAGAAGACTGTTTTCAAGGCATTATCTGCTTTGAAACCCTGAATCCAACCAACAAGAATTTTGTTTCAGTCGATAAAGATGAAATGAATCCAATCCCATCTGACTCGAAAATTTTTGACATCATTGGCCACTTTCTTCATCCCAATCCTGGGACGGAATTACCAAAAACTCCCATCATTTGCAAACCATCAGAGGCTGCCATTGAAAGAGCTCTCAAAATTGCAGGCCTCAACCCTCAGAGAACTCTGTTCTTTGAAGATAGTGTTCGAAACATACAAGCTGGGAAACGTGTTGGGCTCGACACCGTCTTGATTGGCACTTCACAGAGAGTTAAAGGAGCAGATTATGCCTTGGAAAGCATCCACAATCTAAGGGAAGGAATACCTGAGCTTTGGAATGTTGAAATCAAATCAGAAATGGGTTATGCTGGCAAAGTTGGAGTTGAAACTTCAGTGACAGCTTAGAACTACAACATCCTTGTTAGGTTCTTCAAATCCTTCCCCCAAAAAATAATGAAAGAAAAACACAAGAAAAGGGGAAATCTTCCGCCTCCAGTTTCACTGGGCAGCTGTTGTAAATAAGCATTTTGATTATGAGAGTAAAGTTCATAAGTGTAATCTTTTCTGTTTTTCTACAATCACTTAATTAACAAAATCATTGAAAAAAATCACTAAAAAAGATGTTGCCATCTATATTAGATGAATAAAACACAGAGTTCTCATACTCTCCACTAATTTTCTATCCATTCAAATTCATTGCCACCATGTTTTTGCATGGCTGTAGTTCACATGTATCATGTTATGACAACTCCATCCATTCTCTTTACACCTAAATCACATCACGTACTGTTTTATTATCGTAAAATGCATTCTAGACTCAACTCAATATAATTGACAAAAC

Coding sequence (CDS)

ATGGAATACGAGAACCGGTTCAGGCAGGCTCAGAGACCAAAATATGATTGTCTTCTTTTTGATTTAGATGACACTCTGTATCCCTTGAGTTCTGGCATTGCAGCTTCGTGCCTTCAGAATATCAAAGATTACATGGTTGAAAAACTGGGGATAGAGCAGAGTAAAATCCCTGACCTGTGCAATTTACTGTACAAGAACTATGGAACAACCATGGCAGGTCTCAGGGCAATTGGCTATGATTTTGATTATGATGAGTACCACAAGTTTGTTCATGGAAGACTTCCTTATGATAATCTAAAGCCCGATCCTGTTCTAAGGAATCTTCTGTTGAACCTGCCTTATAGGAAAGTTATCTTTACAAATGCTGACAAGATCCATGCAGTTAAAGTTCTTAAAAAACTTGGATTAGAAGACTGTTTTCAAGGCATTATCTGCTTTGAAACCCTGAATCCAACCAACAAGAATTTTGTTTCAGTCGATAAAGATGAAATGAATCCAATCCCATCTGACTCGAAAATTTTTGACATCATTGGCCACTTTCTTCATCCCAATCCTGGGACGGAATTACCAAAAACTCCCATCATTTGCAAACCATCAGAGGCTGCCATTGAAAGAGCTCTCAAAATTGCAGGCCTCAACCCTCAGAGAACTCTGTTCTTTGAAGATAGTGTTCGAAACATACAAGCTGGGAAACGTGTTGGGCTCGACACCGTCTTGATTGGCACTTCACAGAGAGTTAAAGGAGCAGATTATGCCTTGGAAAGCATCCACAATCTAAGGGAAGGAATACCTGAGCTTTGGAATGTTGAAATCAAATCAGAAATGGGTTATGCTGGCAAAGTTGGAGTTGAAACTTCAGTGACAGCTTAG

Protein sequence

MEYENRFRQAQRPKYDCLLFDLDDTLYPLSSGIAASCLQNIKDYMVEKLGIEQSKIPDLCNLLYKNYGTTMAGLRAIGYDFDYDEYHKFVHGRLPYDNLKPDPVLRNLLLNLPYRKVIFTNADKIHAVKVLKKLGLEDCFQGIICFETLNPTNKNFVSVDKDEMNPIPSDSKIFDIIGHFLHPNPGTELPKTPIICKPSEAAIERALKIAGLNPQRTLFFEDSVRNIQAGKRVGLDTVLIGTSQRVKGADYALESIHNLREGIPELWNVEIKSEMGYAGKVGVETSVTA*

Homology

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BLAST of CSPI03G32250 vs. ExPASy Swiss-Prot
Match: Q09893 (Uncharacterized protein C24B11.05 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC24B11.05 PE=3 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 4.8e-16
Identity = 52/129 (40.31%), Postives = 73/129 (56.59%), Query Frame = 0


Query: 18  LLFDLDDTLYPLSSGIAASCLQNIKDYMVEKLGIEQSKIPDLCNLLYKNYGTTMAGLRAI 77
           + FDLD+ LYP S  I       I  +  +KLGI   +   L  + Y++YG  + GL  +
Sbjct: 8   IFFDLDNCLYPKSYKIHNMMAARITAFFSDKLGIPTEEAERLREVYYRHYGIAIRGL-VL 67

Query: 78  GYDFDYDEYHKFVHGRLPYDN-LKPDPVLRNLLLNL--PYRKVIFTNADKIHAVKVLKKL 137
            ++ D  +Y + V   LP +  +K D VLR +LL L   Y+  IFTNA  +HA +VLK L
Sbjct: 68  HHEIDAVDYDQRVDQSLPLEKVIKKDEVLREMLLELRKKYKCWIFTNAYIVHANRVLKYL 127

Query: 138 GLEDCFQGI 144
           G+EDCF GI
Sbjct: 128 GIEDCFDGI 135

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BLAST of CSPI03G32250 vs. ExPASy Swiss-Prot
Match: P53078 (Suppressor of disruption of TFIIS OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SDT1 PE=1 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 1.1e-15
Identity = 68/236 (28.81%), Postives = 103/236 (43.64%), Query Frame = 0


Query: 11  QRPKYDCLLFDLDDTLYPLSSGIAASCLQNIKDYMVEKLGIEQSKIPDLCNLLYKNYGTT 70
           Q P      FD+D+ LY  S+ I     Q+I  +    L +       L N  YK YG  
Sbjct: 51  QNPNLKVFFFDIDNCLYKSSTRIHDLMQQSILRFFQTHLKLSPEDAHVLNNSYYKEYGLA 110

Query: 71  MAGLRAIGYDFDYDEYHKFVHGRLP-YDNLKPDPVLRNLLLNLPY-----RKVIFTNADK 130
           + GL  + +  +  EY++ V   LP  D LKPD  LRN+LL L       +  +FTNA K
Sbjct: 111 IRGL-VMFHKVNALEYNRLVDDSLPLQDILKPDIPLRNMLLRLRQSGKIDKLWLFTNAYK 170

Query: 131 IHAVKVLKKLGLEDCFQGIICFETLNPTNKNFVSVDKDEMNPIPSDSKIFDIIGHFLHPN 190
            HA++ L+ LG+ D F G+        T  ++   D                        
Sbjct: 171 NHAIRCLRLLGIADLFDGL--------TYCDYSRTD------------------------ 230

Query: 191 PGTELPKTPIICKPSEAAIERALKIAGL-NPQRTLFFEDSVRNIQAGKRVGLDTVL 240
                    ++CKP   A E+A+K +GL   +   F +DS +NI+ G ++G+ T +
Sbjct: 231 --------TLVCKPHVKAFEKAMKESGLARYENAYFIDDSGKNIETGIKLGMKTCI 245

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BLAST of CSPI03G32250 vs. ExPASy Swiss-Prot
Match: P40025 (Phosphate metabolism protein 8 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=PHM8 PE=1 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 9.1e-15
Identity = 49/153 (32.03%), Postives = 77/153 (50.33%), Query Frame = 0


Query: 13  PKYDCLLFDLDDTLYPLSSGIAASCLQNIKDYMVEKLGIEQSKIPDLCNLLYKNYGTTMA 72
           P      FD+D+TLY  S+ +     Q++ ++   +LG +  +   L    Y+ YG ++ 
Sbjct: 50  PARKVFFFDIDNTLYRKSTKVQLLMQQSLSNFFKYELGFDDDEAERLIESYYQEYGLSVK 109

Query: 73  GLRAIGYDFDYDEYHKFVHGRLP-YDNLKPDPVLRNLLLNLPYRKV-------IFTNADK 132
           GL       D  +Y+ F+   LP  D LKPD  LR LL+NL  +K+       +FTN+ K
Sbjct: 110 GLIKNKQIDDVLQYNTFIDDSLPLQDYLKPDWKLRELLINLKKKKLGKFDKLWLFTNSYK 169

Query: 133 IHAVKVLKKLGLEDCFQGIICFETLNPTNKNFV 158
            HA++ +K LG+ D F GI       P  + F+
Sbjct: 170 NHAIRCVKILGIADLFDGITYCHYDRPIEEEFI 202

[Close]

BLAST of CSPI03G32250 vs. ExPASy TrEMBL
Match: A0A0A0LD56 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G734170 PE=4 SV=1)

HSP 1 Score: 598.2 bits (1541), Expect = 1.8e-167
Identity = 289/289 (100.00%), Postives = 289/289 (100.00%), Query Frame = 0


Query: 1   MEYENRFRQAQRPKYDCLLFDLDDTLYPLSSGIAASCLQNIKDYMVEKLGIEQSKIPDLC 60
           MEYENRFRQAQRPKYDCLLFDLDDTLYPLSSGIAASCLQNIKDYMVEKLGIEQSKIPDLC
Sbjct: 1   MEYENRFRQAQRPKYDCLLFDLDDTLYPLSSGIAASCLQNIKDYMVEKLGIEQSKIPDLC 60

Query: 61  NLLYKNYGTTMAGLRAIGYDFDYDEYHKFVHGRLPYDNLKPDPVLRNLLLNLPYRKVIFT 120
           NLLYKNYGTTMAGLRAIGYDFDYDEYHKFVHGRLPYDNLKPDPVLRNLLLNLPYRKVIFT
Sbjct: 61  NLLYKNYGTTMAGLRAIGYDFDYDEYHKFVHGRLPYDNLKPDPVLRNLLLNLPYRKVIFT 120

Query: 121 NADKIHAVKVLKKLGLEDCFQGIICFETLNPTNKNFVSVDKDEMNPIPSDSKIFDIIGHF 180
           NADKIHAVKVLKKLGLEDCFQGIICFETLNPTNKNFVSVDKDEMNPIPSDSKIFDIIGHF
Sbjct: 121 NADKIHAVKVLKKLGLEDCFQGIICFETLNPTNKNFVSVDKDEMNPIPSDSKIFDIIGHF 180

Query: 181 LHPNPGTELPKTPIICKPSEAAIERALKIAGLNPQRTLFFEDSVRNIQAGKRVGLDTVLI 240
           LHPNPGTELPKTPIICKPSEAAIERALKIAGLNPQRTLFFEDSVRNIQAGKRVGLDTVLI
Sbjct: 181 LHPNPGTELPKTPIICKPSEAAIERALKIAGLNPQRTLFFEDSVRNIQAGKRVGLDTVLI 240

Query: 241 GTSQRVKGADYALESIHNLREGIPELWNVEIKSEMGYAGKVGVETSVTA 290
           GTSQRVKGADYALESIHNLREGIPELWNVEIKSEMGYAGKVGVETSVTA
Sbjct: 241 GTSQRVKGADYALESIHNLREGIPELWNVEIKSEMGYAGKVGVETSVTA 289

[Close]

BLAST of CSPI03G32250 vs. ExPASy TrEMBL
Match: A0A5A7TPS2 (Suppressor of disruption of TFIIS-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G001620 PE=4 SV=1)

HSP 1 Score: 581.3 bits (1497), Expect = 2.3e-162
Identity = 283/289 (97.92%), Postives = 285/289 (98.62%), Query Frame = 0


Query: 1   MEYENRFRQAQRPKYDCLLFDLDDTLYPLSSGIAASCLQNIKDYMVEKLGIEQSKIPDLC 60
           MEYENRFRQAQRPKYDCLLFDLDDTLYPLSSGIAASCLQNIKDYMVEKLGIEQSKIPDLC
Sbjct: 1   MEYENRFRQAQRPKYDCLLFDLDDTLYPLSSGIAASCLQNIKDYMVEKLGIEQSKIPDLC 60

Query: 61  NLLYKNYGTTMAGLRAIGYDFDYDEYHKFVHGRLPYDNLKPDPVLRNLLLNLPYRKVIFT 120
           NLLYKNYGTTMAGLRAIGY+FDYDEYHKFVHGRLPYDNLKPDPVLRNLLLNLPYRKVIFT
Sbjct: 61  NLLYKNYGTTMAGLRAIGYNFDYDEYHKFVHGRLPYDNLKPDPVLRNLLLNLPYRKVIFT 120

Query: 121 NADKIHAVKVLKKLGLEDCFQGIICFETLNPTNKNFVSVDKDEMNPIPSDSKIFDIIGHF 180
           NADKIHAVKVLKKLGLEDCFQGIICFETLNPTNKNFV    DEMNPIPSDS+IFDIIGHF
Sbjct: 121 NADKIHAVKVLKKLGLEDCFQGIICFETLNPTNKNFV----DEMNPIPSDSEIFDIIGHF 180

Query: 181 LHPNPGTELPKTPIICKPSEAAIERALKIAGLNPQRTLFFEDSVRNIQAGKRVGLDTVLI 240
           LHPNPGTELPKTPIICKPSEAAIERALKIAGLNPQRTLFFEDSVRNIQAGKRVGLDTVLI
Sbjct: 181 LHPNPGTELPKTPIICKPSEAAIERALKIAGLNPQRTLFFEDSVRNIQAGKRVGLDTVLI 240

Query: 241 GTSQRVKGADYALESIHNLREGIPELWNVEIKSEMGYAGKVGVETSVTA 290
           GTSQRVKGADYALESIHNLREGIPELWNVEIKSEMGYAGKVGVETSVTA
Sbjct: 241 GTSQRVKGADYALESIHNLREGIPELWNVEIKSEMGYAGKVGVETSVTA 285

[Close]

BLAST of CSPI03G32250 vs. ExPASy TrEMBL
Match: A0A1S3B6P5 (suppressor of disruption of TFIIS-like OS=Cucumis melo OX=3656 GN=LOC103486381 PE=4 SV=1)

HSP 1 Score: 581.3 bits (1497), Expect = 2.3e-162
Identity = 283/289 (97.92%), Postives = 285/289 (98.62%), Query Frame = 0


Query: 1   MEYENRFRQAQRPKYDCLLFDLDDTLYPLSSGIAASCLQNIKDYMVEKLGIEQSKIPDLC 60
           MEYENRFRQAQRPKYDCLLFDLDDTLYPLSSGIAASCLQNIKDYMVEKLGIEQSKIPDLC
Sbjct: 1   MEYENRFRQAQRPKYDCLLFDLDDTLYPLSSGIAASCLQNIKDYMVEKLGIEQSKIPDLC 60

Query: 61  NLLYKNYGTTMAGLRAIGYDFDYDEYHKFVHGRLPYDNLKPDPVLRNLLLNLPYRKVIFT 120
           NLLYKNYGTTMAGLRAIGY+FDYDEYHKFVHGRLPYDNLKPDPVLRNLLLNLPYRKVIFT
Sbjct: 61  NLLYKNYGTTMAGLRAIGYNFDYDEYHKFVHGRLPYDNLKPDPVLRNLLLNLPYRKVIFT 120

Query: 121 NADKIHAVKVLKKLGLEDCFQGIICFETLNPTNKNFVSVDKDEMNPIPSDSKIFDIIGHF 180
           NADKIHAVKVLKKLGLEDCFQGIICFETLNPTNKNFV    DEMNPIPSDS+IFDIIGHF
Sbjct: 121 NADKIHAVKVLKKLGLEDCFQGIICFETLNPTNKNFV----DEMNPIPSDSEIFDIIGHF 180

Query: 181 LHPNPGTELPKTPIICKPSEAAIERALKIAGLNPQRTLFFEDSVRNIQAGKRVGLDTVLI 240
           LHPNPGTELPKTPIICKPSEAAIERALKIAGLNPQRTLFFEDSVRNIQAGKRVGLDTVLI
Sbjct: 181 LHPNPGTELPKTPIICKPSEAAIERALKIAGLNPQRTLFFEDSVRNIQAGKRVGLDTVLI 240

Query: 241 GTSQRVKGADYALESIHNLREGIPELWNVEIKSEMGYAGKVGVETSVTA 290
           GTSQRVKGADYALESIHNLREGIPELWNVEIKSEMGYAGKVGVETSVTA
Sbjct: 241 GTSQRVKGADYALESIHNLREGIPELWNVEIKSEMGYAGKVGVETSVTA 285

[Close]

BLAST of CSPI03G32250 vs. ExPASy TrEMBL
Match: A0A6J1CWA9 (suppressor of disruption of TFIIS-like OS=Momordica charantia OX=3673 GN=LOC111014990 PE=4 SV=1)

HSP 1 Score: 557.0 bits (1434), Expect = 4.7e-155
Identity = 270/294 (91.84%), Postives = 280/294 (95.24%), Query Frame = 0


Query: 1   MEYENRFRQAQRPKYDCLLFDLDDTLYPLSSGIAASCLQNIKDYMVEKLGIEQSKIPDLC 60
           MEYENRFRQAQRPKYDCLLFDLDDTLYPLS+GIAASCLQNIKDYMVEKLGIEQSKIPD C
Sbjct: 1   MEYENRFRQAQRPKYDCLLFDLDDTLYPLSTGIAASCLQNIKDYMVEKLGIEQSKIPDYC 60

Query: 61  NLLYKNYGTTMAGLRAIGYDFDYDEYHKFVHGRLPYDNLKPDPVLRNLLLNLPYRKVIFT 120
           NLLYKNYGTTMAGLRAIGY+FDYDEYH FVHGRLPYDNLKPDPVLR+LLL+LPYR+VIFT
Sbjct: 61  NLLYKNYGTTMAGLRAIGYNFDYDEYHSFVHGRLPYDNLKPDPVLRSLLLSLPYRRVIFT 120

Query: 121 NADKIHAVKVLKKLGLEDCFQGIICFETLNPTNKNFVSVDKDEM-----NPIPSDSKIFD 180
           NADK HAVKVLKKLGLEDCF+GIICFETLNPTNKNFVSVD+DE+     NP PS S+IFD
Sbjct: 121 NADKDHAVKVLKKLGLEDCFEGIICFETLNPTNKNFVSVDEDELEFMGSNPAPSGSEIFD 180

Query: 181 IIGHFLHPNPGTELPKTPIICKPSEAAIERALKIAGLNPQRTLFFEDSVRNIQAGKRVGL 240
           IIGHFL PNPG ELPKTPIICKPSEAAIERALKIAGLNPQRTLFFEDSVRNIQAGKRVGL
Sbjct: 181 IIGHFLQPNPGVELPKTPIICKPSEAAIERALKIAGLNPQRTLFFEDSVRNIQAGKRVGL 240

Query: 241 DTVLIGTSQRVKGADYALESIHNLREGIPELWNVEIKSEMGYAGKVGVETSVTA 290
           DTVLIGTS R+KGADYALESIHNLREGIPELWNVEIKSEMGYAGKV VETSVTA
Sbjct: 241 DTVLIGTSHRIKGADYALESIHNLREGIPELWNVEIKSEMGYAGKVTVETSVTA 294

[Close]

BLAST of CSPI03G32250 vs. ExPASy TrEMBL
Match: A0A6J1GF49 (uncharacterized protein LOC111453617 OS=Cucurbita moschata OX=3662 GN=LOC111453617 PE=4 SV=1)

HSP 1 Score: 533.1 bits (1372), Expect = 7.2e-148
Identity = 262/296 (88.51%), Postives = 276/296 (93.24%), Query Frame = 0


Query: 1   MEYENRFRQAQRPKYDCLLFDLDDTLYPLSSGIAASCLQNIKDYMVEKLGIEQSKIPDLC 60
           MEYENRFRQAQRPKYDCLLFDLDDTLYPLS+GIAA+CLQNIKDYMVEKLGIE SKI DLC
Sbjct: 1   MEYENRFRQAQRPKYDCLLFDLDDTLYPLSTGIAAACLQNIKDYMVEKLGIEPSKIADLC 60

Query: 61  NLLYKNYGTTMAGLRAIGYDFDYDEYHKFVHGRLPYDNLKPDPVLRNLLLNLPYRKVIFT 120
           NLLYKNYGTTMAGLRAIGY+FDYDEYH FVHGRLPYDNLKPDPVLR+LLL+LPYRK+IFT
Sbjct: 61  NLLYKNYGTTMAGLRAIGYEFDYDEYHSFVHGRLPYDNLKPDPVLRSLLLSLPYRKIIFT 120

Query: 121 NADKIHAVKVLKKLGLEDCFQGIICFETLNPTNKNFVSVDKDE------MNP-IPSDSKI 180
           NAD+IH+V+VLK+LGLEDCF+GIICFETLNPTNKNFVS D+D+       NP  PS S+I
Sbjct: 121 NADRIHSVEVLKRLGLEDCFEGIICFETLNPTNKNFVSADEDDELEFLGSNPTAPSGSEI 180

Query: 181 FDIIGHFLHPNPGTELPKTPIICKPSEAAIERALKIAGLNPQRTLFFEDSVRNIQAGKRV 240
           FDIIGHFL PNPG ELPKTPIICKPSEAAIERALKIAG   QRTLFFEDSVRNIQAGKRV
Sbjct: 181 FDIIGHFLQPNPGVELPKTPIICKPSEAAIERALKIAG--AQRTLFFEDSVRNIQAGKRV 240

Query: 241 GLDTVLIGTSQRVKGADYALESIHNLREGIPELWNVEIKSEMGYAGKVGVETSVTA 290
           GLDTVLIGTSQRVKGADYALESIHNLREGIPELWNVEIKSEMGYAGKV VETSVTA
Sbjct: 241 GLDTVLIGTSQRVKGADYALESIHNLREGIPELWNVEIKSEMGYAGKVAVETSVTA 294

[Close]

BLAST of CSPI03G32250 vs. NCBI nr
Match: XP_011651891.1 (suppressor of disruption of TFIIS [Cucumis sativus] >KGN58864.1 hypothetical protein Csa_000976 [Cucumis sativus])

HSP 1 Score: 598.2 bits (1541), Expect = 3.8e-167
Identity = 289/289 (100.00%), Postives = 289/289 (100.00%), Query Frame = 0


Query: 1   MEYENRFRQAQRPKYDCLLFDLDDTLYPLSSGIAASCLQNIKDYMVEKLGIEQSKIPDLC 60
           MEYENRFRQAQRPKYDCLLFDLDDTLYPLSSGIAASCLQNIKDYMVEKLGIEQSKIPDLC
Sbjct: 1   MEYENRFRQAQRPKYDCLLFDLDDTLYPLSSGIAASCLQNIKDYMVEKLGIEQSKIPDLC 60

Query: 61  NLLYKNYGTTMAGLRAIGYDFDYDEYHKFVHGRLPYDNLKPDPVLRNLLLNLPYRKVIFT 120
           NLLYKNYGTTMAGLRAIGYDFDYDEYHKFVHGRLPYDNLKPDPVLRNLLLNLPYRKVIFT
Sbjct: 61  NLLYKNYGTTMAGLRAIGYDFDYDEYHKFVHGRLPYDNLKPDPVLRNLLLNLPYRKVIFT 120

Query: 121 NADKIHAVKVLKKLGLEDCFQGIICFETLNPTNKNFVSVDKDEMNPIPSDSKIFDIIGHF 180
           NADKIHAVKVLKKLGLEDCFQGIICFETLNPTNKNFVSVDKDEMNPIPSDSKIFDIIGHF
Sbjct: 121 NADKIHAVKVLKKLGLEDCFQGIICFETLNPTNKNFVSVDKDEMNPIPSDSKIFDIIGHF 180

Query: 181 LHPNPGTELPKTPIICKPSEAAIERALKIAGLNPQRTLFFEDSVRNIQAGKRVGLDTVLI 240
           LHPNPGTELPKTPIICKPSEAAIERALKIAGLNPQRTLFFEDSVRNIQAGKRVGLDTVLI
Sbjct: 181 LHPNPGTELPKTPIICKPSEAAIERALKIAGLNPQRTLFFEDSVRNIQAGKRVGLDTVLI 240

Query: 241 GTSQRVKGADYALESIHNLREGIPELWNVEIKSEMGYAGKVGVETSVTA 290
           GTSQRVKGADYALESIHNLREGIPELWNVEIKSEMGYAGKVGVETSVTA
Sbjct: 241 GTSQRVKGADYALESIHNLREGIPELWNVEIKSEMGYAGKVGVETSVTA 289

[Close]

BLAST of CSPI03G32250 vs. NCBI nr
Match: XP_008442536.1 (PREDICTED: suppressor of disruption of TFIIS-like [Cucumis melo] >KAA0044096.1 suppressor of disruption of TFIIS-like [Cucumis melo var. makuwa] >TYK25041.1 suppressor of disruption of TFIIS-like [Cucumis melo var. makuwa])

HSP 1 Score: 581.3 bits (1497), Expect = 4.8e-162
Identity = 283/289 (97.92%), Postives = 285/289 (98.62%), Query Frame = 0


Query: 1   MEYENRFRQAQRPKYDCLLFDLDDTLYPLSSGIAASCLQNIKDYMVEKLGIEQSKIPDLC 60
           MEYENRFRQAQRPKYDCLLFDLDDTLYPLSSGIAASCLQNIKDYMVEKLGIEQSKIPDLC
Sbjct: 1   MEYENRFRQAQRPKYDCLLFDLDDTLYPLSSGIAASCLQNIKDYMVEKLGIEQSKIPDLC 60

Query: 61  NLLYKNYGTTMAGLRAIGYDFDYDEYHKFVHGRLPYDNLKPDPVLRNLLLNLPYRKVIFT 120
           NLLYKNYGTTMAGLRAIGY+FDYDEYHKFVHGRLPYDNLKPDPVLRNLLLNLPYRKVIFT
Sbjct: 61  NLLYKNYGTTMAGLRAIGYNFDYDEYHKFVHGRLPYDNLKPDPVLRNLLLNLPYRKVIFT 120

Query: 121 NADKIHAVKVLKKLGLEDCFQGIICFETLNPTNKNFVSVDKDEMNPIPSDSKIFDIIGHF 180
           NADKIHAVKVLKKLGLEDCFQGIICFETLNPTNKNFV    DEMNPIPSDS+IFDIIGHF
Sbjct: 121 NADKIHAVKVLKKLGLEDCFQGIICFETLNPTNKNFV----DEMNPIPSDSEIFDIIGHF 180

Query: 181 LHPNPGTELPKTPIICKPSEAAIERALKIAGLNPQRTLFFEDSVRNIQAGKRVGLDTVLI 240
           LHPNPGTELPKTPIICKPSEAAIERALKIAGLNPQRTLFFEDSVRNIQAGKRVGLDTVLI
Sbjct: 181 LHPNPGTELPKTPIICKPSEAAIERALKIAGLNPQRTLFFEDSVRNIQAGKRVGLDTVLI 240

Query: 241 GTSQRVKGADYALESIHNLREGIPELWNVEIKSEMGYAGKVGVETSVTA 290
           GTSQRVKGADYALESIHNLREGIPELWNVEIKSEMGYAGKVGVETSVTA
Sbjct: 241 GTSQRVKGADYALESIHNLREGIPELWNVEIKSEMGYAGKVGVETSVTA 285

[Close]

BLAST of CSPI03G32250 vs. NCBI nr
Match: XP_038903150.1 (suppressor of disruption of TFIIS-like [Benincasa hispida])

HSP 1 Score: 569.7 bits (1467), Expect = 1.4e-158
Identity = 278/294 (94.56%), Postives = 284/294 (96.60%), Query Frame = 0


Query: 1   MEYENRFRQAQRPKYDCLLFDLDDTLYPLSSGIAASCLQNIKDYMVEKLGIEQSKIPDLC 60
           MEYENRFRQAQRPKYDCLLFDLDDTLYPLSSGIAASCLQNIKDYMVEKLGIEQ KIPDLC
Sbjct: 1   MEYENRFRQAQRPKYDCLLFDLDDTLYPLSSGIAASCLQNIKDYMVEKLGIEQRKIPDLC 60

Query: 61  NLLYKNYGTTMAGLRAIGYDFDYDEYHKFVHGRLPYDNLKPDPVLRNLLLNLPYRKVIFT 120
           NLLYKNYGTTMAGLRAIGYDFDYDEYH+FVHGRLPYDNLKPDPVLR+LLL+LPYRKVIFT
Sbjct: 61  NLLYKNYGTTMAGLRAIGYDFDYDEYHRFVHGRLPYDNLKPDPVLRSLLLSLPYRKVIFT 120

Query: 121 NADKIHAVKVLKKLGLEDCFQGIICFETLNPTNKNFVSVDKDEM-----NPIPSDSKIFD 180
           NADKIHAVKVLKKLGLEDCFQGIICFETLNPTNKNFVS DKDE+     NPIPSDS+IFD
Sbjct: 121 NADKIHAVKVLKKLGLEDCFQGIICFETLNPTNKNFVSDDKDELECFGTNPIPSDSEIFD 180

Query: 181 IIGHFLHPNPGTELPKTPIICKPSEAAIERALKIAGLNPQRTLFFEDSVRNIQAGKRVGL 240
           IIG+FL PNPG ELPKTPIICKPSEAAIERALKIAGLNPQRTLFFEDSVRNIQAGKRVGL
Sbjct: 181 IIGYFLQPNPGMELPKTPIICKPSEAAIERALKIAGLNPQRTLFFEDSVRNIQAGKRVGL 240

Query: 241 DTVLIGTSQRVKGADYALESIHNLREGIPELWNVEIKSEMGYAGKVGVETSVTA 290
           DTVLIGTSQRVKGADYALESIHNLREGIPELWNVEIKSEMGYAGKV VETSVTA
Sbjct: 241 DTVLIGTSQRVKGADYALESIHNLREGIPELWNVEIKSEMGYAGKVAVETSVTA 294

[Close]

BLAST of CSPI03G32250 vs. NCBI nr
Match: XP_022145568.1 (suppressor of disruption of TFIIS-like [Momordica charantia])

HSP 1 Score: 557.0 bits (1434), Expect = 9.7e-155
Identity = 270/294 (91.84%), Postives = 280/294 (95.24%), Query Frame = 0


Query: 1   MEYENRFRQAQRPKYDCLLFDLDDTLYPLSSGIAASCLQNIKDYMVEKLGIEQSKIPDLC 60
           MEYENRFRQAQRPKYDCLLFDLDDTLYPLS+GIAASCLQNIKDYMVEKLGIEQSKIPD C
Sbjct: 1   MEYENRFRQAQRPKYDCLLFDLDDTLYPLSTGIAASCLQNIKDYMVEKLGIEQSKIPDYC 60

Query: 61  NLLYKNYGTTMAGLRAIGYDFDYDEYHKFVHGRLPYDNLKPDPVLRNLLLNLPYRKVIFT 120
           NLLYKNYGTTMAGLRAIGY+FDYDEYH FVHGRLPYDNLKPDPVLR+LLL+LPYR+VIFT
Sbjct: 61  NLLYKNYGTTMAGLRAIGYNFDYDEYHSFVHGRLPYDNLKPDPVLRSLLLSLPYRRVIFT 120

Query: 121 NADKIHAVKVLKKLGLEDCFQGIICFETLNPTNKNFVSVDKDEM-----NPIPSDSKIFD 180
           NADK HAVKVLKKLGLEDCF+GIICFETLNPTNKNFVSVD+DE+     NP PS S+IFD
Sbjct: 121 NADKDHAVKVLKKLGLEDCFEGIICFETLNPTNKNFVSVDEDELEFMGSNPAPSGSEIFD 180

Query: 181 IIGHFLHPNPGTELPKTPIICKPSEAAIERALKIAGLNPQRTLFFEDSVRNIQAGKRVGL 240
           IIGHFL PNPG ELPKTPIICKPSEAAIERALKIAGLNPQRTLFFEDSVRNIQAGKRVGL
Sbjct: 181 IIGHFLQPNPGVELPKTPIICKPSEAAIERALKIAGLNPQRTLFFEDSVRNIQAGKRVGL 240

Query: 241 DTVLIGTSQRVKGADYALESIHNLREGIPELWNVEIKSEMGYAGKVGVETSVTA 290
           DTVLIGTS R+KGADYALESIHNLREGIPELWNVEIKSEMGYAGKV VETSVTA
Sbjct: 241 DTVLIGTSHRIKGADYALESIHNLREGIPELWNVEIKSEMGYAGKVTVETSVTA 294

[Close]

BLAST of CSPI03G32250 vs. NCBI nr
Match: KAG6596404.1 (hypothetical protein SDJN03_09584, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 534.3 bits (1375), Expect = 6.7e-148
Identity = 263/296 (88.85%), Postives = 276/296 (93.24%), Query Frame = 0


Query: 1   MEYENRFRQAQRPKYDCLLFDLDDTLYPLSSGIAASCLQNIKDYMVEKLGIEQSKIPDLC 60
           MEYENRFRQAQRPKYDCLLFDLDDTLYPLS+GIAA+CLQNIKDYMVEKLGIE SKI DLC
Sbjct: 37  MEYENRFRQAQRPKYDCLLFDLDDTLYPLSTGIAAACLQNIKDYMVEKLGIEPSKIADLC 96

Query: 61  NLLYKNYGTTMAGLRAIGYDFDYDEYHKFVHGRLPYDNLKPDPVLRNLLLNLPYRKVIFT 120
           NLLYKNYGTTMAGLRAIGY+FDYDEYH FVHGRLPYDNLKPDPVLR+LLL+LPYRK+IFT
Sbjct: 97  NLLYKNYGTTMAGLRAIGYEFDYDEYHSFVHGRLPYDNLKPDPVLRSLLLSLPYRKIIFT 156

Query: 121 NADKIHAVKVLKKLGLEDCFQGIICFETLNPTNKNFVSVDKDE------MNP-IPSDSKI 180
           NADKIH+V+VLK+LGLEDCF+GIICFETLNPTNKNFVS D+D+       NP  PS S+I
Sbjct: 157 NADKIHSVEVLKRLGLEDCFEGIICFETLNPTNKNFVSADEDDELEFLGSNPTAPSGSEI 216

Query: 181 FDIIGHFLHPNPGTELPKTPIICKPSEAAIERALKIAGLNPQRTLFFEDSVRNIQAGKRV 240
           FDIIGHFL PNPG ELPKTPIICKPSEAAIERALKIAG   QRTLFFEDSVRNIQAGKRV
Sbjct: 217 FDIIGHFLQPNPGVELPKTPIICKPSEAAIERALKIAG--AQRTLFFEDSVRNIQAGKRV 276

Query: 241 GLDTVLIGTSQRVKGADYALESIHNLREGIPELWNVEIKSEMGYAGKVGVETSVTA 290
           GLDTVLIGTSQRVKGADYALESIHNLREGIPELWNVEIKSEMGYAGKV VETSVTA
Sbjct: 277 GLDTVLIGTSQRVKGADYALESIHNLREGIPELWNVEIKSEMGYAGKVAVETSVTA 330

[Close]

BLAST of CSPI03G32250 vs. TAIR 10
Match: AT5G02230.1 (Haloacid dehalogenase-like hydrolase (HAD) superfamily protein )

HSP 1 Score: 418.3 bits (1074), Expect = 5.0e-117
Identity = 203/290 (70.00%), Postives = 237/290 (81.72%), Query Frame = 0


Query: 1   MEYENRFRQAQRPKYDCLLFDLDDTLYPLSSGIAASCLQNIKDYMVEKLGIEQSKIPDLC 60
           ME+ENR+  A   KYDCLLFDLDDTLYPLSSGIA  C  NIKDYM EKLGI + KI +L 
Sbjct: 1   MEFENRYGLATSQKYDCLLFDLDDTLYPLSSGIARECGNNIKDYMTEKLGIPKDKIVELS 60

Query: 61  NLLYKNYGTTMAGLRAIGYDFDYDEYHKFVHGRLPYDNLKPDPVLRNLLLNLPYRKVIFT 120
           +LLYKNYGTTMAGLRAIGY+FDYDEYH FVHGRLPYDN+KPD VLR+LLL+LP RKVIFT
Sbjct: 61  DLLYKNYGTTMAGLRAIGYEFDYDEYHSFVHGRLPYDNIKPDLVLRSLLLSLPLRKVIFT 120

Query: 121 NADKIHAVKVLKKLGLEDCFQGIICFETLNPTNKNFVSVDKDEMNPIPSDSKIFDIIGHF 180
           NAD++HA K LKKLGLEDCF+GIICFETLN  + N  S          ++S+IFDI+GHF
Sbjct: 121 NADRVHAAKALKKLGLEDCFEGIICFETLNLMHTNAAS----------NNSEIFDIVGHF 180

Query: 181 LHPNPGTELPKTPIICKPSEAAIERALKIAGLNPQRTLFFEDSVRNIQAGKRVGLDTVLI 240
               P   LPKTP++CKPSE+AIE+AL+IA ++P RTLFFEDSVRN+QAGKRVGL TVL+
Sbjct: 181 NRSEPVGSLPKTPVVCKPSESAIEKALEIANIDPNRTLFFEDSVRNVQAGKRVGLYTVLV 240

Query: 241 GTSQRVKGADYALESIHNLREGIPELWNVEIK-SEMGYAGKVGVETSVTA 290
           G S +VKGADYALE+IHN++E IPELW  + K S++GY+GKV VETSV A
Sbjct: 241 GKSTKVKGADYALENIHNMKEAIPELWESDRKSSDVGYSGKVAVETSVRA 280

[Close]

BLAST of CSPI03G32250 vs. TAIR 10
Match: AT5G02230.2 (Haloacid dehalogenase-like hydrolase (HAD) superfamily protein )

HSP 1 Score: 418.3 bits (1074), Expect = 5.0e-117
Identity = 203/290 (70.00%), Postives = 237/290 (81.72%), Query Frame = 0


Query: 1   MEYENRFRQAQRPKYDCLLFDLDDTLYPLSSGIAASCLQNIKDYMVEKLGIEQSKIPDLC 60
           ME+ENR+  A   KYDCLLFDLDDTLYPLSSGIA  C  NIKDYM EKLGI + KI +L 
Sbjct: 1   MEFENRYGLATSQKYDCLLFDLDDTLYPLSSGIARECGNNIKDYMTEKLGIPKDKIVELS 60

Query: 61  NLLYKNYGTTMAGLRAIGYDFDYDEYHKFVHGRLPYDNLKPDPVLRNLLLNLPYRKVIFT 120
           +LLYKNYGTTMAGLRAIGY+FDYDEYH FVHGRLPYDN+KPD VLR+LLL+LP RKVIFT
Sbjct: 61  DLLYKNYGTTMAGLRAIGYEFDYDEYHSFVHGRLPYDNIKPDLVLRSLLLSLPLRKVIFT 120

Query: 121 NADKIHAVKVLKKLGLEDCFQGIICFETLNPTNKNFVSVDKDEMNPIPSDSKIFDIIGHF 180
           NAD++HA K LKKLGLEDCF+GIICFETLN  + N  S          ++S+IFDI+GHF
Sbjct: 121 NADRVHAAKALKKLGLEDCFEGIICFETLNLMHTNAAS----------NNSEIFDIVGHF 180

Query: 181 LHPNPGTELPKTPIICKPSEAAIERALKIAGLNPQRTLFFEDSVRNIQAGKRVGLDTVLI 240
               P   LPKTP++CKPSE+AIE+AL+IA ++P RTLFFEDSVRN+QAGKRVGL TVL+
Sbjct: 181 NRSEPVGSLPKTPVVCKPSESAIEKALEIANIDPNRTLFFEDSVRNVQAGKRVGLYTVLV 240

Query: 241 GTSQRVKGADYALESIHNLREGIPELWNVEIK-SEMGYAGKVGVETSVTA 290
           G S +VKGADYALE+IHN++E IPELW  + K S++GY+GKV VETSV A
Sbjct: 241 GKSTKVKGADYALENIHNMKEAIPELWESDRKSSDVGYSGKVAVETSVRA 280

[Close]

BLAST of CSPI03G32250 vs. TAIR 10
Match: AT5G59480.1 (Haloacid dehalogenase-like hydrolase (HAD) superfamily protein )

HSP 1 Score: 360.1 bits (923), Expect = 1.6e-99
Identity = 167/287 (58.19%), Postives = 213/287 (74.22%), Query Frame = 0


Query: 1   MEYENRFRQAQRPKYDCLLFDLDDTLYPLSSGIAASCLQNIKDYMVEKLGIEQSKIPDLC 60
           M YE  F+Q    KYDCLLFD+DDTLYPLSSG+A    +NI++YMV+KLGIE+ K+ +LC
Sbjct: 1   MAYEEHFQQTSEAKYDCLLFDIDDTLYPLSSGLAMEVKKNIQEYMVQKLGIEEDKVQELC 60

Query: 61  NLLYKNYGTTMAGLRAIGYDFDYDEYHKFVHGRLPYDNLKPDPVLRNLLLNLPYRKVIFT 120
             LYK YGTTMAGL+A+GYDFDYD++H+FVHGRLPY  LKPDP+LRN++L+LP RKV+FT
Sbjct: 61  LSLYKIYGTTMAGLKAVGYDFDYDDFHRFVHGRLPYSTLKPDPILRNIILSLPIRKVVFT 120

Query: 121 NADKIHAVKVLKKLGLEDCFQGIICFETLNPTNKNFVSVDKDEMNPIPSDSKIFDIIGHF 180
           NADK HA K++ +LGLE CF+ II FETLNP  K    VD           +IFDII + 
Sbjct: 121 NADKAHAAKIIARLGLEGCFEKIISFETLNPITKTESPVD-------TKTREIFDIISYM 180

Query: 181 LHPNPGTELPKTPIICKPSEAAIERALKIAGLNPQRTLFFEDSVRNIQAGKRVGLDTVLI 240
            +P+   ELPKT ++CKPSE A E+  K+A +NP++TLFF+DS+RNIQ GKRVGL TV +
Sbjct: 181 ANPDSSIELPKTSVVCKPSEGAFEQVFKMANINPKKTLFFDDSIRNIQTGKRVGLHTVWV 240

Query: 241 GTSQRVKGADYALESIHNLREGIPELWNV--EIKSEMGYAGKVGVET 286
           GTS R +G D ALE IHN+RE +P+LW+   +   E+    KV +ET
Sbjct: 241 GTSHREEGVDIALEHIHNIREALPQLWDAVDDKAKEIRTRQKVAIET 280

[Close]

BLAST of CSPI03G32250 vs. TAIR 10
Match: AT5G59480.2 (Haloacid dehalogenase-like hydrolase (HAD) superfamily protein )

HSP 1 Score: 354.8 bits (909), Expect = 6.8e-98
Identity = 167/287 (58.19%), Postives = 212/287 (73.87%), Query Frame = 0


Query: 1   MEYENRFRQAQRPKYDCLLFDLDDTLYPLSSGIAASCLQNIKDYMVEKLGIEQSKIPDLC 60
           M YE  F+Q    KYDCLLFD+DDTLYPLSSG+A    +NI++YMV+KLGIE+ K+ +LC
Sbjct: 1   MAYEEHFQQTSEAKYDCLLFDIDDTLYPLSSGLAMEVKKNIQEYMVQKLGIEEDKVQELC 60

Query: 61  NLLYKNYGTTMAGLRAIGYDFDYDEYHKFVHGRLPYDNLKPDPVLRNLLLNLPYRKVIFT 120
             LYK YGTTMAGL+A+GYDFDYD++H+FVHGRLPY  LKPDP+LRN++L+LP RKV FT
Sbjct: 61  LSLYKIYGTTMAGLKAVGYDFDYDDFHRFVHGRLPYSTLKPDPILRNIILSLPIRKV-FT 120

Query: 121 NADKIHAVKVLKKLGLEDCFQGIICFETLNPTNKNFVSVDKDEMNPIPSDSKIFDIIGHF 180
           NADK HA K++ +LGLE CF+ II FETLNP  K    VD           +IFDII + 
Sbjct: 121 NADKAHAAKIIARLGLEGCFEKIISFETLNPITKTESPVD-------TKTREIFDIISYM 180

Query: 181 LHPNPGTELPKTPIICKPSEAAIERALKIAGLNPQRTLFFEDSVRNIQAGKRVGLDTVLI 240
            +P+   ELPKT ++CKPSE A E+  K+A +NP++TLFF+DS+RNIQ GKRVGL TV +
Sbjct: 181 ANPDSSIELPKTSVVCKPSEGAFEQVFKMANINPKKTLFFDDSIRNIQTGKRVGLHTVWV 240

Query: 241 GTSQRVKGADYALESIHNLREGIPELWNV--EIKSEMGYAGKVGVET 286
           GTS R +G D ALE IHN+RE +P+LW+   +   E+    KV +ET
Sbjct: 241 GTSHREEGVDIALEHIHNIREALPQLWDAVDDKAKEIRTRQKVAIET 279

[Close]

BLAST of CSPI03G32250 vs. TAIR 10
Match: AT5G59490.1 (Haloacid dehalogenase-like hydrolase (HAD) superfamily protein )

HSP 1 Score: 328.2 bits (840), Expect = 6.8e-90
Identity = 155/288 (53.82%), Postives = 209/288 (72.57%), Query Frame = 0


Query: 7   FRQAQRPKYDCLLFDLDDTLYPLSSGIAASCLQNIKDYMVEKLGIEQSKIPDLCNLLYKN 66
           F  +  P+Y+CLLFDLDDTLYPLSSG++ +C  NI +YMVEKLGI++  + +L  +LYK 
Sbjct: 3   FVNSSPPRYECLLFDLDDTLYPLSSGLSDACSNNIIEYMVEKLGIDEDGVVELNQILYKK 62

Query: 67  YGTTMAGLRAIGYDFDYDEYHKFVHGRLPYDNLKPDPVLRNLLLNLPYRKVIFTNADKIH 126
           YGT+MAGL+A+GY+FD DEYH++VHGRLPY+NLKPDPVLR+LLL LP RK++F+N D++H
Sbjct: 63  YGTSMAGLKAVGYEFDNDEYHRYVHGRLPYENLKPDPVLRSLLLGLPLRKLVFSNGDEVH 122

Query: 127 AVKVLKKLGLEDCFQGIICFETLNPTNKNFVSVDKDEMNPIPSDSKIFDIIGHFLHPNPG 186
            +K L +LG+EDCF+ II FETLNP           ++N    ++++  + GH       
Sbjct: 123 VMKALTRLGIEDCFERIISFETLNP-----------DIN----EAELSCVTGH------- 182

Query: 187 TELPKTPIICKPSEAAIERALKIAGLNPQRTLFFEDSVRNIQAGKRVGLDTVLIGTSQRV 246
             LP+ P+ICKP+E A E+A  IA LNP +TLFF+DS RNIQ GK VGL TVL+G S+++
Sbjct: 183 --LPENPVICKPTEIAFEKAFDIAQLNPHKTLFFDDSTRNIQTGKAVGLHTVLVGKSEKI 242

Query: 247 KGADYALESIHNLREGIPELWNVEI-----KSEMGYAGKVGVETSVTA 290
            G+DYALESIHN++E  PELW+  I        + YA ++ +ETSV A
Sbjct: 243 DGSDYALESIHNMKEAFPELWSESIINNKETERIDYASQISIETSVQA 266

The following BLAST results are available for this feature:

BLAST of CSPI03G32250 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q09893	4.8e-16	40.31	Uncharacterized protein C24B11.05 OS=Schizosaccharomyces pombe (strain 972 / ATC...	[more]
P53078	1.1e-15	28.81	Suppressor of disruption of TFIIS OS=Saccharomyces cerevisiae (strain ATCC 20450...	[more]
P40025	9.1e-15	32.03	Phosphate metabolism protein 8 OS=Saccharomyces cerevisiae (strain ATCC 204508 /...	[more]

BLAST of CSPI03G32250 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LD56	1.8e-167	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G734170 PE=4 SV=1	[more]
A0A5A7TPS2	2.3e-162	97.92	Suppressor of disruption of TFIIS-like OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A1S3B6P5	2.3e-162	97.92	suppressor of disruption of TFIIS-like OS=Cucumis melo OX=3656 GN=LOC103486381 P...	[more]
A0A6J1CWA9	4.7e-155	91.84	suppressor of disruption of TFIIS-like OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A6J1GF49	7.2e-148	88.51	uncharacterized protein LOC111453617 OS=Cucurbita moschata OX=3662 GN=LOC1114536...	[more]

BLAST of CSPI03G32250 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011651891.1	3.8e-167	100.00	suppressor of disruption of TFIIS [Cucumis sativus] >KGN58864.1 hypothetical pro...	[more]
XP_008442536.1	4.8e-162	97.92	PREDICTED: suppressor of disruption of TFIIS-like [Cucumis melo] >KAA0044096.1 s...	[more]
XP_038903150.1	1.4e-158	94.56	suppressor of disruption of TFIIS-like [Benincasa hispida]	[more]
XP_022145568.1	9.7e-155	91.84	suppressor of disruption of TFIIS-like [Momordica charantia]	[more]
KAG6596404.1	6.7e-148	88.85	hypothetical protein SDJN03_09584, partial [Cucurbita argyrosperma subsp. sorori...	[more]

BLAST of CSPI03G32250 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G02230.1	5.0e-117	70.00	Haloacid dehalogenase-like hydrolase (HAD) superfamily protein	[more]
AT5G02230.2	5.0e-117	70.00	Haloacid dehalogenase-like hydrolase (HAD) superfamily protein	[more]
AT5G59480.1	1.6e-99	58.19	Haloacid dehalogenase-like hydrolase (HAD) superfamily protein	[more]
AT5G59480.2	6.8e-98	58.19	Haloacid dehalogenase-like hydrolase (HAD) superfamily protein	[more]
AT5G59490.1	6.8e-90	53.82	Haloacid dehalogenase-like hydrolase (HAD) superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR023214	HAD superfamily	GENE3D	3.40.50.1000		coord: 81..267 e-value: 8.1E-46 score: 157.6
IPR041492	Haloacid dehalogenase-like hydrolase	PFAM	PF13419	HAD_2	coord: 197..239 e-value: 2.1E-5 score: 24.7
IPR006439	HAD hydrolase, subfamily IA	TIGRFAM	TIGR01509	TIGR01509	coord: 195..240 e-value: 2.1E-10 score: 38.9
IPR010237	Pyrimidine 5-nucleotidase	TIGRFAM	TIGR01993	TIGR01993	coord: 16..239 e-value: 4.7E-68 score: 226.5
IPR010237	Pyrimidine 5-nucleotidase	SFLD	SFLDG01132	C1.5.3:_5'-Nucleotidase_Like	coord: 10..268 e-value: 0.0 score: 237.0
IPR010237	Pyrimidine 5-nucleotidase	CDD	cd02604	HAD_5NT	coord: 17..242 e-value: 2.95921E-81 score: 241.383
None	No IPR available	SFLD	SFLDG01129	C1.5:_HAD,_Beta-PGM,_Phosphat	coord: 10..268 e-value: 0.0 score: 237.0
None	No IPR available	PANTHER	PTHR12725:SF82	HALOACID DEHALOGENASE-LIKE HYDROLASE (HAD) SUPERFAMILY PROTEIN	coord: 1..289
None	No IPR available	PANTHER	PTHR12725	HALOACID DEHALOGENASE-LIKE HYDROLASE	coord: 1..289
IPR036412	HAD-like superfamily	SUPERFAMILY	56784	HAD-like	coord: 13..267

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G32250.1	CSPI03G32250.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016787	hydrolase activity