CSPI03G32240 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI03G32240
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	cyclin-dependent protein kinase inhibitor SMR4
Location	Chr3: 28823489 .. 28824235 (-)
RNA-Seq Expression	CSPI03G32240
Synteny	CSPI03G32240

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAGGGGGTGAAGGGAATGGAAGGGAAGGGAAGGGAAGGGAAGACCCACAGTCAACAATTGACGCGTAGACGACTTCTCATTTCTTAGCTGTCTCCCACATCGACGCCACTCCCCGCCATTCATTTATTTTTCTGTTTCTTTTCAAACACACTCCCAATTTTTCAAAATCAAATTCTCATTCTCCGCCTCCTCCTCTTCTCCTTCTCGCCACCGTCCACGCCCGCGCTCTGTTCGTAGTTTCGAATTTTCATTACCTTCTTCTAAAACTTTACTCAATAAACCTCTACCTCTTACTTTTCATTTCCTTGGCTATGGACTCGAAGCAGGGGACTGGCAATTACGAGGAAGAGTGTTCGACGCCGACCCGGTGGGAGTGTCGGATTCCGGCGGTCTTTAACTGTCCTCCGCCCCCGAAGAAAAAGAATGCAACCGCTGGAAAGAAGCGGGAACCACCGAAACATGGCTATTTTCAGCCACCTGATCTTGAGGCTCTGTTTTCTGTTCCTCCCAGAAGGGAAGCCTGCGCTTAAATTTTATGCTCTGTTTTAGGGTTTTAAGGAAATGGGTTTTTCTTTTTTTGCTTCTTTCTTCTGTTTGATTAGATTGTTCTATTGAATTGTGAATACATTTTGAGAGTTTGAAAGTTGATTTGGGTTTTTTTTTTTTTAGTTTGCCATTTATGAGAATTTGTATTGAGAATGGAAGAATCTTGGGTTGAAATATTATGGGTTAGTTTTAATTGGGGAA

mRNA sequence

AAGGGGGTGAAGGGAATGGAAGGGAAGGGAAGGGAAGGGAAGACCCACAGTCAACAATTGACGCGTAGACGACTTCTCATTTCTTAGCTGTCTCCCACATCGACGCCACTCCCCGCCATTCATTTATTTTTCTGTTTCTTTTCAAACACACTCCCAATTTTTCAAAATCAAATTCTCATTCTCCGCCTCCTCCTCTTCTCCTTCTCGCCACCGTCCACGCCCGCGCTCTGTTCGTAGTTTCGAATTTTCATTACCTTCTTCTAAAACTTTACTCAATAAACCTCTACCTCTTACTTTTCATTTCCTTGGCTATGGACTCGAAGCAGGGGACTGGCAATTACGAGGAAGAGTGTTCGACGCCGACCCGGTGGGAGTGTCGGATTCCGGCGGTCTTTAACTGTCCTCCGCCCCCGAAGAAAAAGAATGCAACCGCTGGAAAGAAGCGGGAACCACCGAAACATGGCTATTTTCAGCCACCTGATCTTGAGGCTCTGTTTTCTGTTCCTCCCAGAAGGGAAGCCTGCGCTTAAATTTTATGCTCTGTTTTAGGGTTTTAAGGAAATGGGTTTTTCTTTTTTTGCTTCTTTCTTCTGTTTGATTAGATTGTTCTATTGAATTGTGAATACATTTTGAGAGTTTGAAAGTTGATTTGGGTTTTTTTTTTTTTAGTTTGCCATTTATGAGAATTTGTATTGAGAATGGAAGAATCTTGGGTTGAAATATTATGGGTTAGTTTTAATTGGGGAA

Coding sequence (CDS)

ATGGACTCGAAGCAGGGGACTGGCAATTACGAGGAAGAGTGTTCGACGCCGACCCGGTGGGAGTGTCGGATTCCGGCGGTCTTTAACTGTCCTCCGCCCCCGAAGAAAAAGAATGCAACCGCTGGAAAGAAGCGGGAACCACCGAAACATGGCTATTTTCAGCCACCTGATCTTGAGGCTCTGTTTTCTGTTCCTCCCAGAAGGGAAGCCTGCGCTTAA

Protein sequence

MDSKQGTGNYEEECSTPTRWECRIPAVFNCPPPPKKKNATAGKKREPPKHGYFQPPDLEALFSVPPRREACA*

Homology

[Close]

BLAST of CSPI03G32240 vs. ExPASy Swiss-Prot
Match: Q1JPP8 (Cyclin-dependent protein kinase inhibitor SMR4 OS=Arabidopsis thaliana OX=3702 GN=SMR4 PE=1 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 2.5e-14
Identity = 37/62 (59.68%), Postives = 43/62 (69.35%), Query Frame = 0


Query: 11 EEECSTPTRWECRIPAVFNCPPPPKKKNATAGKKREPPKHGYFQPPDLEALFSVPPRREA 70
          EE C+TP     RIP    CPPPP+KK     +KR+PP++GYFQPPDLE LF   PRREA
Sbjct: 12 EEGCTTPRSTMYRIPVASVCPPPPRKKLMVV-RKRDPPRNGYFQPPDLETLFYAQPRREA 71

Query: 71 CA 73
          CA
Sbjct: 72 CA 72

[Close]

BLAST of CSPI03G32240 vs. ExPASy Swiss-Prot
Match: Q9LNX4 (Cyclin-dependent protein kinase inhibitor SMR5 OS=Arabidopsis thaliana OX=3702 GN=SMR5 PE=1 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 1.7e-13
Identity = 36/64 (56.25%), Postives = 45/64 (70.31%), Query Frame = 0


Query: 9  NYEEECSTPTRWECRIPAVFNCPPPPKKKNATA--GKKREPPKHGYFQPPDLEALFSVPP 68
          +Y+  C+TPTR +CRIPA   CPPP ++K +    GKKREPPK GYFQPPDL+  FSV  
Sbjct: 17 DYQMGCTTPTRDDCRIPAYPPCPPPVRRKRSLLGFGKKREPPKKGYFQPPDLDLFFSVVA 76

Query: 69 RREA 71
            +A
Sbjct: 77 ASQA 80

[Close]

BLAST of CSPI03G32240 vs. ExPASy Swiss-Prot
Match: Q29Q81 (Cyclin-dependent protein kinase inhibitor SMR6 OS=Arabidopsis thaliana OX=3702 GN=SMR6 PE=1 SV=1)

HSP 1 Score: 43.9 bits (102), Expect = 9.1e-04
Identity = 27/56 (48.21%), Postives = 33/56 (58.93%), Query Frame = 0


Query: 9   NYEEECS-TPTRWECRIPAVFNCPPPPKKKNATAGKKREPPKHGYFQPP-DLEALF 63
           N EEECS TPT  E +IP +  CPP P+K+   A K R      +F PP DLE +F
Sbjct: 54  NEEEECSTTPTAKETKIPELLECPPAPRKRR-PALKCRSNVVREFFTPPSDLETVF 108

[Close]

BLAST of CSPI03G32240 vs. ExPASy TrEMBL
Match: A0A0A0LAJ2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G734160 PE=4 SV=1)

HSP 1 Score: 161.8 bits (408), Expect = 1.1e-36
Identity = 71/72 (98.61%), Postives = 72/72 (100.00%), Query Frame = 0


Query: 1   MDSKQGTGNYEEECSTPTRWECRIPAVFNCPPPPKKKNATAGKKREPPKHGYFQPPDLEA 60
           MDSKQGTGNYEEECSTPTRWECRIPAVFNCPPPPKKKNATAGKKREPPKHGYFQPPDLEA
Sbjct: 87  MDSKQGTGNYEEECSTPTRWECRIPAVFNCPPPPKKKNATAGKKREPPKHGYFQPPDLEA 146

Query: 61  LFSVPPRREACA 73
           LFS+PPRREACA
Sbjct: 147 LFSIPPRREACA 158

[Close]

BLAST of CSPI03G32240 vs. ExPASy TrEMBL
Match: A0A6J1J1Y0 (cyclin-dependent protein kinase inhibitor SMR4 OS=Cucurbita maxima OX=3661 GN=LOC111481954 PE=4 SV=1)

HSP 1 Score: 139.4 bits (350), Expect = 5.9e-30
Identity = 63/74 (85.14%), Postives = 66/74 (89.19%), Query Frame = 0


Query: 1  MDSKQGTGNYEEECSTPTRWECRIPAVFNCPPPP---KKKNATAGKKREPPKHGYFQPPD 60
          M++KQG  NYEEECSTPTRWECRIPAVFNCPPPP   KKK   +GKKREPPKHGYFQPPD
Sbjct: 1  MEAKQGPANYEEECSTPTRWECRIPAVFNCPPPPPPKKKKAVGSGKKREPPKHGYFQPPD 60

Query: 61 LEALFSVPPRREAC 72
          LEALFSVPPRREAC
Sbjct: 61 LEALFSVPPRREAC 74

[Close]

BLAST of CSPI03G32240 vs. ExPASy TrEMBL
Match: A0A7N2M7Y9 (Uncharacterized protein OS=Quercus lobata OX=97700 PE=4 SV=1)

HSP 1 Score: 96.3 bits (238), Expect = 5.7e-17
Identity = 43/62 (69.35%), Postives = 53/62 (85.48%), Query Frame = 0


Query: 11 EEECSTPTRWECRIPAVFNCPPPPKKKNATAGKKREPPKHGYFQPPDLEALFSVPPRREA 70
          E+ CSTP R EC+IP V  CPPPPKKK+A +G KR+PPK+GYFQPPDL+A+F++PPRREA
Sbjct: 8  EDRCSTPKRRECQIPVVVVCPPPPKKKSA-SGMKRDPPKNGYFQPPDLDAIFTMPPRREA 67

Query: 71 CA 73
           A
Sbjct: 68 WA 68

[Close]

BLAST of CSPI03G32240 vs. ExPASy TrEMBL
Match: I1KK06 (Uncharacterized protein OS=Glycine max OX=3847 GN=GLYMA_07G133800 PE=4 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 4.8e-16
Identity = 40/62 (64.52%), Postives = 49/62 (79.03%), Query Frame = 0


Query: 10 YEEECSTPTRWECRIPAVFNCPPPPKKKNATAGKKREPPKHGYFQPPDLEALFSVPPRRE 69
          +E+EC TP   + +IPAVF CPPPP +K   AGK+R PPK GYFQPPDL++LFSVP R+E
Sbjct: 3  WEDECRTPRLVQNQIPAVFICPPPPPRKKPVAGKRRPPPKEGYFQPPDLDSLFSVPARKE 62

Query: 70 AC 72
          AC
Sbjct: 63 AC 64

[Close]

BLAST of CSPI03G32240 vs. ExPASy TrEMBL
Match: A0A445JWA4 (Cyclin-dependent protein kinase inhibitor SMR4 OS=Glycine soja OX=3848 GN=D0Y65_017753 PE=4 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 4.8e-16
Identity = 40/62 (64.52%), Postives = 49/62 (79.03%), Query Frame = 0


Query: 10 YEEECSTPTRWECRIPAVFNCPPPPKKKNATAGKKREPPKHGYFQPPDLEALFSVPPRRE 69
          +E+EC TP   + +IPAVF CPPPP +K   AGK+R PPK GYFQPPDL++LFSVP R+E
Sbjct: 3  WEDECRTPRLVQNQIPAVFICPPPPPRKKPVAGKRRPPPKEGYFQPPDLDSLFSVPARKE 62

Query: 70 AC 72
          AC
Sbjct: 63 AC 64

[Close]

BLAST of CSPI03G32240 vs. NCBI nr
Match: XP_031737830.1 (cyclin-dependent protein kinase inhibitor SMR4 [Cucumis sativus] >KAE8651008.1 hypothetical protein Csa_002137 [Cucumis sativus])

HSP 1 Score: 161.8 bits (408), Expect = 2.3e-36
Identity = 71/72 (98.61%), Postives = 72/72 (100.00%), Query Frame = 0


Query: 1  MDSKQGTGNYEEECSTPTRWECRIPAVFNCPPPPKKKNATAGKKREPPKHGYFQPPDLEA 60
          MDSKQGTGNYEEECSTPTRWECRIPAVFNCPPPPKKKNATAGKKREPPKHGYFQPPDLEA
Sbjct: 1  MDSKQGTGNYEEECSTPTRWECRIPAVFNCPPPPKKKNATAGKKREPPKHGYFQPPDLEA 60

Query: 61 LFSVPPRREACA 73
          LFS+PPRREACA
Sbjct: 61 LFSIPPRREACA 72

[Close]

BLAST of CSPI03G32240 vs. NCBI nr
Match: XP_038905412.1 (cyclin-dependent protein kinase inhibitor SMR4 [Benincasa hispida])

HSP 1 Score: 146.4 bits (368), Expect = 9.9e-32
Identity = 64/72 (88.89%), Postives = 67/72 (93.06%), Query Frame = 0


Query: 1  MDSKQGTGNYEEECSTPTRWECRIPAVFNCPPPPKKKNATAGKKREPPKHGYFQPPDLEA 60
          M++KQG  NYEEECSTPTRWECRIPAVFNCPPPPKKK  T GKKR+PPKHGYFQPPDLEA
Sbjct: 1  MEAKQGPANYEEECSTPTRWECRIPAVFNCPPPPKKKIGTDGKKRDPPKHGYFQPPDLEA 60

Query: 61 LFSVPPRREACA 73
          LFSVPPRREACA
Sbjct: 61 LFSVPPRREACA 72

[Close]

BLAST of CSPI03G32240 vs. NCBI nr
Match: XP_023528630.1 (cyclin-dependent protein kinase inhibitor SMR4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 140.6 bits (353), Expect = 5.4e-30
Identity = 63/74 (85.14%), Postives = 67/74 (90.54%), Query Frame = 0


Query: 1  MDSKQGTGNYEEECSTPTRWECRIPAVFNCPPPP---KKKNATAGKKREPPKHGYFQPPD 60
          M++KQG  NYEEECSTPTRWECRIPAVFNCPPPP   KKK  ++GKKREPPKHGYFQPPD
Sbjct: 1  MEAKQGPANYEEECSTPTRWECRIPAVFNCPPPPPPKKKKAVSSGKKREPPKHGYFQPPD 60

Query: 61 LEALFSVPPRREAC 72
          LEALFSVPPRREAC
Sbjct: 61 LEALFSVPPRREAC 74

[Close]

BLAST of CSPI03G32240 vs. NCBI nr
Match: XP_022983341.1 (cyclin-dependent protein kinase inhibitor SMR4 [Cucurbita maxima])

HSP 1 Score: 139.4 bits (350), Expect = 1.2e-29
Identity = 63/74 (85.14%), Postives = 66/74 (89.19%), Query Frame = 0


Query: 1  MDSKQGTGNYEEECSTPTRWECRIPAVFNCPPPP---KKKNATAGKKREPPKHGYFQPPD 60
          M++KQG  NYEEECSTPTRWECRIPAVFNCPPPP   KKK   +GKKREPPKHGYFQPPD
Sbjct: 1  MEAKQGPANYEEECSTPTRWECRIPAVFNCPPPPPPKKKKAVGSGKKREPPKHGYFQPPD 60

Query: 61 LEALFSVPPRREAC 72
          LEALFSVPPRREAC
Sbjct: 61 LEALFSVPPRREAC 74

[Close]

BLAST of CSPI03G32240 vs. NCBI nr
Match: KAG6580535.1 (Cyclin-dependent protein kinase inhibitor SMR4, partial [Cucurbita argyrosperma subsp. sororia] >KAG7017288.1 Cyclin-dependent protein kinase inhibitor SMR4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 137.5 bits (345), Expect = 4.6e-29
Identity = 62/74 (83.78%), Postives = 66/74 (89.19%), Query Frame = 0


Query: 1  MDSKQGTGNYEEECSTPTRWECRIPAVFNCPPPP---KKKNATAGKKREPPKHGYFQPPD 60
          M++KQG  NYEEECSTPTRWECRIPAV NCPPPP   KKK  ++GKKREPPKHGYFQPPD
Sbjct: 1  MEAKQGPANYEEECSTPTRWECRIPAVSNCPPPPPPKKKKAVSSGKKREPPKHGYFQPPD 60

Query: 61 LEALFSVPPRREAC 72
          LEALFSVPPRREAC
Sbjct: 61 LEALFSVPPRREAC 74

[Close]

BLAST of CSPI03G32240 vs. TAIR 10
Match: AT5G02220.1 (unknown protein; Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 79.0 bits (193), Expect = 1.8e-15
Identity = 37/62 (59.68%), Postives = 43/62 (69.35%), Query Frame = 0


Query: 11 EEECSTPTRWECRIPAVFNCPPPPKKKNATAGKKREPPKHGYFQPPDLEALFSVPPRREA 70
          EE C+TP     RIP    CPPPP+KK     +KR+PP++GYFQPPDLE LF   PRREA
Sbjct: 12 EEGCTTPRSTMYRIPVASVCPPPPRKKLMVV-RKRDPPRNGYFQPPDLETLFYAQPRREA 71

Query: 71 CA 73
          CA
Sbjct: 72 CA 72

[Close]

BLAST of CSPI03G32240 vs. TAIR 10
Match: AT5G40460.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G27630.1); Has 87 Blast hits to 87 proteins in 12 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 87; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 43.9 bits (102), Expect = 6.4e-05
Identity = 27/56 (48.21%), Postives = 33/56 (58.93%), Query Frame = 0


Query: 9   NYEEECS-TPTRWECRIPAVFNCPPPPKKKNATAGKKREPPKHGYFQPP-DLEALF 63
           N EEECS TPT  E +IP +  CPP P+K+   A K R      +F PP DLE +F
Sbjct: 54  NEEEECSTTPTAKETKIPELLECPPAPRKRR-PALKCRSNVVREFFTPPSDLETVF 108

The following BLAST results are available for this feature:

BLAST of CSPI03G32240 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q1JPP8	2.5e-14	59.68	Cyclin-dependent protein kinase inhibitor SMR4 OS=Arabidopsis thaliana OX=3702 G...	[more]
Q9LNX4	1.7e-13	56.25	Cyclin-dependent protein kinase inhibitor SMR5 OS=Arabidopsis thaliana OX=3702 G...	[more]
Q29Q81	9.1e-04	48.21	Cyclin-dependent protein kinase inhibitor SMR6 OS=Arabidopsis thaliana OX=3702 G...	[more]

BLAST of CSPI03G32240 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LAJ2	1.1e-36	98.61	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G734160 PE=4 SV=1	[more]
A0A6J1J1Y0	5.9e-30	85.14	cyclin-dependent protein kinase inhibitor SMR4 OS=Cucurbita maxima OX=3661 GN=LO...	[more]
A0A7N2M7Y9	5.7e-17	69.35	Uncharacterized protein OS=Quercus lobata OX=97700 PE=4 SV=1	[more]
I1KK06	4.8e-16	64.52	Uncharacterized protein OS=Glycine max OX=3847 GN=GLYMA_07G133800 PE=4 SV=1	[more]
A0A445JWA4	4.8e-16	64.52	Cyclin-dependent protein kinase inhibitor SMR4 OS=Glycine soja OX=3848 GN=D0Y65_...	[more]

BLAST of CSPI03G32240 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_031737830.1	2.3e-36	98.61	cyclin-dependent protein kinase inhibitor SMR4 [Cucumis sativus] >KAE8651008.1 h...	[more]
XP_038905412.1	9.9e-32	88.89	cyclin-dependent protein kinase inhibitor SMR4 [Benincasa hispida]	[more]
XP_023528630.1	5.4e-30	85.14	cyclin-dependent protein kinase inhibitor SMR4 [Cucurbita pepo subsp. pepo]	[more]
XP_022983341.1	1.2e-29	85.14	cyclin-dependent protein kinase inhibitor SMR4 [Cucurbita maxima]	[more]
KAG6580535.1	4.6e-29	83.78	Cyclin-dependent protein kinase inhibitor SMR4, partial [Cucurbita argyrosperma ...	[more]

BLAST of CSPI03G32240 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G02220.1	1.8e-15	59.68	unknown protein; Has 30201 Blast hits to 17322 proteins in 780 species: Archae -...	[more]
AT5G40460.1	6.4e-05	48.21	unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 32..58
None	No IPR available	PANTHER	PTHR33142:SF15	OS01G0843700 PROTEIN	coord: 1..70
IPR040389	Cyclin-dependent protein kinase inhibitor SMR	PANTHER	PTHR33142	CYCLIN-DEPENDENT PROTEIN KINASE INHIBITOR SMR13	coord: 1..70

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G32240.1	CSPI03G32240.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032875	regulation of DNA endoreduplication