CSPI03G32100 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI03G32100
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	LRR receptor-like serine/threonine-protein kinase EFR
Location	Chr3: 28768373 .. 28768954 (+)
RNA-Seq Expression	CSPI03G32100
Synteny	CSPI03G32100

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAGGTCTCAATTTTATTACTTCACTTACCAATTCAACTAGCCTGAAAGTGTTGTCTGTGGCCACAAATTCACTCACGTGTCAGCTTCCACGGTCCATTGGAAATTGGTTAACTCAACTCTATACGCTTCTAATAGCAGAAAACCAGTTTAGTGGAACCATTCCTGAAGAAATAGGAAATTTGGTTGGTCTCATTTCTGTATCCTTCCTTACAAATTCCTTCACAGGGAACATTCCTTTTCCATTAGAAAACCTCAAGAATTTGCAGATATTGTCTTTAAAATCCAATTTTCTTTCAGGCTTCATTCCAGAAACATTTGGTAATCTAAGTGAGCCATCTCAATTCACAGTTAATGACAACAACTTAGGTGGCAAAATTCCTCCAAGTTTAACAAACTGTGAGCGTCTCACCATTTTTTATATTTCAGAAAACGGGTTAATTGGAAACTTACCCGATGAGATATTTAACTTTAAAACTCTTTTAATATTCAATGTTTCATTCAACAATTTTACAGGTCACTTGCCTTCTCAAATTGACAAGTTATCGATGGTTCAACAGTTTGATGTTGCCAACAATTAA

mRNA sequence

ATGGAAGGTCTCAATTTTATTACTTCACTTACCAATTCAACTAGCCTGAAAGTGTTGTCTGTGGCCACAAATTCACTCACGTGTCAGCTTCCACGGTCCATTGGAAATTGGTTAACTCAACTCTATACGCTTCTAATAGCAGAAAACCAGTTTAGTGGAACCATTCCTGAAGAAATAGGAAATTTGGTTGGTCTCATTTCTGTATCCTTCCTTACAAATTCCTTCACAGGGAACATTCCTTTTCCATTAGAAAACCTCAAGAATTTGCAGATATTGTCTTTAAAATCCAATTTTCTTTCAGGCTTCATTCCAGAAACATTTGGTAATCTAAGTGAGCCATCTCAATTCACAGTTAATGACAACAACTTAGGTGGCAAAATTCCTCCAAGTTTAACAAACTGTGAGCGTCTCACCATTTTTTATATTTCAGAAAACGGGTTAATTGGAAACTTACCCGATGAGATATTTAACTTTAAAACTCTTTTAATATTCAATGTTTCATTCAACAATTTTACAGGTCACTTGCCTTCTCAAATTGACAAGTTATCGATGGTTCAACAGTTTGATGTTGCCAACAATTAA

Coding sequence (CDS)

ATGGAAGGTCTCAATTTTATTACTTCACTTACCAATTCAACTAGCCTGAAAGTGTTGTCTGTGGCCACAAATTCACTCACGTGTCAGCTTCCACGGTCCATTGGAAATTGGTTAACTCAACTCTATACGCTTCTAATAGCAGAAAACCAGTTTAGTGGAACCATTCCTGAAGAAATAGGAAATTTGGTTGGTCTCATTTCTGTATCCTTCCTTACAAATTCCTTCACAGGGAACATTCCTTTTCCATTAGAAAACCTCAAGAATTTGCAGATATTGTCTTTAAAATCCAATTTTCTTTCAGGCTTCATTCCAGAAACATTTGGTAATCTAAGTGAGCCATCTCAATTCACAGTTAATGACAACAACTTAGGTGGCAAAATTCCTCCAAGTTTAACAAACTGTGAGCGTCTCACCATTTTTTATATTTCAGAAAACGGGTTAATTGGAAACTTACCCGATGAGATATTTAACTTTAAAACTCTTTTAATATTCAATGTTTCATTCAACAATTTTACAGGTCACTTGCCTTCTCAAATTGACAAGTTATCGATGGTTCAACAGTTTGATGTTGCCAACAATTAA

Protein sequence

MEGLNFITSLTNSTSLKVLSVATNSLTCQLPRSIGNWLTQLYTLLIAENQFSGTIPEEIGNLVGLISVSFLTNSFTGNIPFPLENLKNLQILSLKSNFLSGFIPETFGNLSEPSQFTVNDNNLGGKIPPSLTNCERLTIFYISENGLIGNLPDEIFNFKTLLIFNVSFNNFTGHLPSQIDKLSMVQQFDVANN*

Homology

[Close]

BLAST of CSPI03G32100 vs. ExPASy Swiss-Prot
Match: C0LGT6 (LRR receptor-like serine/threonine-protein kinase EFR OS=Arabidopsis thaliana OX=3702 GN=EFR PE=1 SV=1)

HSP 1 Score: 139.8 bits (351), Expect = 3.2e-32
Identity = 74/183 (40.44%), Postives = 104/183 (56.83%), Query Frame = 0


Query: 3   GLNFITSLTNSTSLKVLSVATNSLTCQLPRSIGNWLTQLYTLLIAENQFSGTIPEEIGNL 62
           GL FI ++ N T L+ L V  N L  +LP SI N  T L +L + +N  SGTIP +IGNL
Sbjct: 333 GLEFIGAVANCTQLEYLDVGYNRLGGELPASIANLSTTLTSLFLGQNLISGTIPHDIGNL 392

Query: 63  VGLISVSFLTNSFTGNIPFPLENLKNLQILSLKSNFLSGFIPETFGNLSEPSQFTVNDNN 122
           V L  +S  TN  +G +P     L NLQ++ L SN +SG IP  FGN++   +  +N N+
Sbjct: 393 VSLQELSLETNMLSGELPVSFGKLLNLQVVDLYSNAISGEIPSYFGNMTRLQKLHLNSNS 452

Query: 123 LGGKIPPSLTNCERLTIFYISENGLIGNLPDEIFNFKTLLIFNVSFNNFTGHLPSQIDKL 182
             G+IP SL  C  L   ++  N L G +P EI    +L   ++S N  TGH P ++ KL
Sbjct: 453 FHGRIPQSLGRCRYLLDLWMDTNRLNGTIPQEILQIPSLAYIDLSNNFLTGHFPEEVGKL 512

Query: 183 SMV 186
            ++
Sbjct: 513 ELL 515

[Close]

BLAST of CSPI03G32100 vs. ExPASy Swiss-Prot
Match: Q1MX30 (Receptor kinase-like protein Xa21 OS=Oryza sativa subsp. indica OX=39946 GN=XA21 PE=1 SV=1)

HSP 1 Score: 131.7 bits (330), Expect = 8.8e-30
Identity = 75/189 (39.68%), Postives = 108/189 (57.14%), Query Frame = 0


Query: 6   FITSLTNSTSLKVLSVATNSLTCQLPRSIGNWLTQLYTLLIAENQFSGTIPEEIGNLVGL 65
           FI+ LTN + L+ L++  N+L   LP S  N  T L  L +  N+ +G+IP++IGNL+GL
Sbjct: 343 FISDLTNCSKLQTLNLGENNLGGVLPNSFSNLSTSLSFLALELNKITGSIPKDIGNLIGL 402

Query: 66  ISVSFLTNSFTGNIPFPLENLKNLQILSLKSNFLSGFIPETFGNLSEPSQFTVNDNNLGG 125
             +    N+F G++P  L  LKNL IL    N LSG IP   GNL+E +   +  N   G
Sbjct: 403 QHLYLCNNNFRGSLPSSLGRLKNLGILLAYENNLSGSIPLAIGNLTELNILLLGTNKFSG 462

Query: 126 KIPPSLTNCERLTIFYISENGLIGNLPDEIFNFKTL-LIFNVSFNNFTGHLPSQIDKLSM 185
            IP +L+N   L    +S N L G +P E+FN +TL ++ NVS NN  G +P +I  L  
Sbjct: 463 WIPYTLSNLTNLLSLGLSTNNLSGPIPSELFNIQTLSIMINVSKNNLEGSIPQEIGHLKN 522

Query: 186 VQQFDVANN 194
           + +F   +N
Sbjct: 523 LVEFHAESN 531

[Close]

BLAST of CSPI03G32100 vs. ExPASy Swiss-Prot
Match: Q2R2D5 (Receptor kinase-like protein Xa21 OS=Oryza sativa subsp. japonica OX=39947 GN=XA21 PE=1 SV=1)

HSP 1 Score: 130.6 bits (327), Expect = 2.0e-29
Identity = 75/193 (38.86%), Postives = 108/193 (55.96%), Query Frame = 0


Query: 2   EGLNFITSLTNSTSLKVLSVATNSLTCQLPRSIGNWLTQLYTLLIAENQFSGTIPEEIGN 61
           E   FI+ LTN + L+ L +  N+L   LP S  N  T L  L +  N+ +G+IP++IGN
Sbjct: 351 EDWGFISDLTNCSKLQTLDLGENNLGGVLPNSFSNLSTSLSFLALDLNKITGSIPKDIGN 410

Query: 62  LVGLISVSFLTNSFTGNIPFPLENLKNLQILSLKSNFLSGFIPETFGNLSEPSQFTVNDN 121
           L+GL  +    N+F G++P  L  L+NL IL    N LSG IP   GNL+E +   +  N
Sbjct: 411 LIGLQHLYLCNNNFRGSLPSSLGRLRNLGILVAYENNLSGSIPLAIGNLTELNILLLGTN 470

Query: 122 NLGGKIPPSLTNCERLTIFYISENGLIGNLPDEIFNFKTL-LIFNVSFNNFTGHLPSQID 181
              G IP +L+N   L    +S N L G +P E+FN +TL ++ NVS NN  G +P +I 
Sbjct: 471 KFSGWIPYTLSNLTNLLSLGLSTNNLSGPIPSELFNIQTLSIMINVSKNNLEGSIPQEIG 530

Query: 182 KLSMVQQFDVANN 194
            L  + +F   +N
Sbjct: 531 HLKNLVEFHAESN 543

[Close]

BLAST of CSPI03G32100 vs. ExPASy Swiss-Prot
Match: Q9LP24 (Probable leucine-rich repeat receptor-like protein kinase At1g35710 OS=Arabidopsis thaliana OX=3702 GN=At1g35710 PE=1 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 2.2e-28
Identity = 68/173 (39.31%), Postives = 101/173 (58.38%), Query Frame = 0


Query: 10  LTNSTSLKVLSVATNSLTCQLPRSIGNWLTQLYTLLIAENQFSGTIPEEIGNLVGLISVS 69
           L N  S+  L+++ N LT  +P ++GN L  L  L + EN  +G IP EIGN+  + +++
Sbjct: 218 LGNMESMTDLALSQNKLTGSIPSTLGN-LKNLMVLYLYENYLTGVIPPEIGNMESMTNLA 277

Query: 70  FLTNSFTGNIPFPLENLKNLQILSLKSNFLSGFIPETFGNLSEPSQFTVNDNNLGGKIPP 129
              N  TG+IP  L NLKNL +LSL  N+L+G IP   GN+       +++N L G IP 
Sbjct: 278 LSQNKLTGSIPSSLGNLKNLTLLSLFQNYLTGGIPPKLGNIESMIDLELSNNKLTGSIPS 337

Query: 130 SLTNCERLTIFYISENGLIGNLPDEIFNFKTLLIFNVSFNNFTGHLPSQIDKL 183
           SL N + LTI Y+ EN L G +P E+ N ++++   ++ N  TG +PS    L
Sbjct: 338 SLGNLKNLTILYLYENYLTGVIPPELGNMESMIDLQLNNNKLTGSIPSSFGNL 389

[Close]

BLAST of CSPI03G32100 vs. ExPASy Swiss-Prot
Match: Q9SRL7 (Receptor-like protein 35 OS=Arabidopsis thaliana OX=3702 GN=RLP35 PE=3 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 3.7e-28
Identity = 72/186 (38.71%), Postives = 104/186 (55.91%), Query Frame = 0


Query: 8   TSLTNSTSLKVLSVATNSLTCQLPRSIGNWLTQLYTLLIAENQFSGTIPEEIGNLVGLIS 67
           +S+ N + L  L ++ N     +P SI N L++L +L ++ NQFSG IP  IGNL  L S
Sbjct: 143 SSIGNLSHLTSLHLSYNQFLGLIPSSIEN-LSRLTSLHLSSNQFSGQIPSSIGNLSHLTS 202

Query: 68  VSFLTNSFTGNIPFPLENLKNLQILSLKSNFLSGFIPETFGNLSEPSQFTVNDNNLGGKI 127
           +   +N F+G IP  + NL NL  LSL SN   G IP + GNL+  +   ++ NN  G+I
Sbjct: 203 LELSSNQFSGQIPSSIGNLSNLTFLSLPSNDFFGQIPSSIGNLARLTYLYLSYNNFVGEI 262

Query: 128 PPSLTNCERLTIFYISENGLIGNLPDEIFNFKTLLIFNVSFNNFTGHLPSQIDKLSMVQQ 187
           P S  N  +L +  +  N L GN+P  + N   L    +S N FTG +P+ I  LS +  
Sbjct: 263 PSSFGNLNQLIVLQVDSNKLSGNVPISLLNLTRLSALLLSHNQFTGTIPNNISLLSNLMD 322

Query: 188 FDVANN 194
           F+ +NN
Sbjct: 323 FEASNN 327

[Close]

BLAST of CSPI03G32100 vs. ExPASy TrEMBL
Match: A0A0A0LAH4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G734020 PE=4 SV=1)

HSP 1 Score: 372.5 bits (955), Expect = 1.1e-99
Identity = 187/187 (100.00%), Postives = 187/187 (100.00%), Query Frame = 0


Query: 1   MEGLNFITSLTNSTSLKVLSVATNSLTCQLPRSIGNWLTQLYTLLIAENQFSGTIPEEIG 60
           MEGLNFITSLTNSTSLKVLSVATNSLTCQLPRSIGNWLTQLYTLLIAENQFSGTIPEEIG
Sbjct: 1   MEGLNFITSLTNSTSLKVLSVATNSLTCQLPRSIGNWLTQLYTLLIAENQFSGTIPEEIG 60

Query: 61  NLVGLISVSFLTNSFTGNIPFPLENLKNLQILSLKSNFLSGFIPETFGNLSEPSQFTVND 120
           NLVGLISVSFLTNSFTGNIPFPLENLKNLQILSLKSNFLSGFIPETFGNLSEPSQFTVND
Sbjct: 61  NLVGLISVSFLTNSFTGNIPFPLENLKNLQILSLKSNFLSGFIPETFGNLSEPSQFTVND 120

Query: 121 NNLGGKIPPSLTNCERLTIFYISENGLIGNLPDEIFNFKTLLIFNVSFNNFTGHLPSQID 180
           NNLGGKIPPSLTNCERLTIFYISENGLIGNLPDEIFNFKTLLIFNVSFNNFTGHLPSQID
Sbjct: 121 NNLGGKIPPSLTNCERLTIFYISENGLIGNLPDEIFNFKTLLIFNVSFNNFTGHLPSQID 180

Query: 181 KLSMVQQ 188
           KLSMVQQ
Sbjct: 181 KLSMVQQ 187

[Close]

BLAST of CSPI03G32100 vs. ExPASy TrEMBL
Match: A0A6J1I5C7 (LRR receptor-like serine/threonine-protein kinase EFR OS=Cucurbita maxima OX=3661 GN=LOC111470057 PE=3 SV=1)

HSP 1 Score: 268.1 bits (684), Expect = 2.9e-68
Identity = 143/192 (74.48%), Postives = 154/192 (80.21%), Query Frame = 0


Query: 2   EGLNFITSLTNSTSLKVLSVATNSLTCQLPRSIGNWLTQLYTLLIAENQFSGTIPEEIGN 61
           EGLNFITSLTNST LK+LSVATN LT QLPRSIGN       LL+AENQFSG+IP EIGN
Sbjct: 332 EGLNFITSLTNSTHLKMLSVATNLLTGQLPRSIGNLSNHCSKLLMAENQFSGSIPSEIGN 391

Query: 62  LVGLISVSFLTNSFTGNIPFPLENLKNLQILSLKSNFLSGFIPETFGNLSEPSQFTVNDN 121
           L GLISVS  +NSFTGNIP  L NLKNLQ+LSL+SN LSG IPETFGNLSE S F VNDN
Sbjct: 392 LGGLISVSLPSNSFTGNIPSSLSNLKNLQVLSLESNILSGSIPETFGNLSELSLFLVNDN 451

Query: 122 NLGGKIPPSLTNCERLTIFYISENGLIGNLPDEIFNFKTLLIFNVSFNNFTGHLPSQIDK 181
            L GKIP SLTNCERL +F +S+NGL GNLP EIF+F  LL FNVS NN TG LPS+IDK
Sbjct: 452 KLSGKIPLSLTNCERLLVFDLSQNGLSGNLPKEIFSFPNLLEFNVSVNNLTGSLPSEIDK 511

Query: 182 LSMVQQFDVANN 194
           L MVQ FDVA N
Sbjct: 512 LLMVQLFDVATN 523

[Close]

BLAST of CSPI03G32100 vs. ExPASy TrEMBL
Match: A0A6J1HAI5 (LRR receptor-like serine/threonine-protein kinase EFR OS=Cucurbita moschata OX=3662 GN=LOC111461581 PE=3 SV=1)

HSP 1 Score: 266.9 bits (681), Expect = 6.5e-68
Identity = 142/192 (73.96%), Postives = 154/192 (80.21%), Query Frame = 0


Query: 2   EGLNFITSLTNSTSLKVLSVATNSLTCQLPRSIGNWLTQLYTLLIAENQFSGTIPEEIGN 61
           EGLNFITSL NST LK+LSVATN LT QLPRSIGN    +  LL+AENQFSG+IP EIGN
Sbjct: 332 EGLNFITSLINSTHLKMLSVATNLLTGQLPRSIGNLSAHISKLLMAENQFSGSIPSEIGN 391

Query: 62  LVGLISVSFLTNSFTGNIPFPLENLKNLQILSLKSNFLSGFIPETFGNLSEPSQFTVNDN 121
           L GLISVS  +NSFTGNIP  L NLKNLQ+LSL+SN LSG IPETFGNLSE S F VNDN
Sbjct: 392 LGGLISVSLPSNSFTGNIPSSLSNLKNLQVLSLESNILSGSIPETFGNLSELSLFLVNDN 451

Query: 122 NLGGKIPPSLTNCERLTIFYISENGLIGNLPDEIFNFKTLLIFNVSFNNFTGHLPSQIDK 181
            L GKIP SLTNCERL +F +S+NGL GNLP EIF+F  LL FNVS NN TG LPS+IDK
Sbjct: 452 KLSGKIPLSLTNCERLLVFDLSQNGLSGNLPKEIFSFPNLLEFNVSVNNLTGSLPSEIDK 511

Query: 182 LSMVQQFDVANN 194
           L MVQ FDVA N
Sbjct: 512 LLMVQLFDVATN 523

[Close]

BLAST of CSPI03G32100 vs. ExPASy TrEMBL
Match: A0A6J1CXN3 (uncharacterized protein LOC111015147 OS=Momordica charantia OX=3673 GN=LOC111015147 PE=3 SV=1)

HSP 1 Score: 199.5 bits (506), Expect = 1.3e-47
Identity = 104/194 (53.61%), Postives = 138/194 (71.13%), Query Frame = 0


Query: 2   EGLNFITSLTNSTSLKVLSVATNSLTCQLPRSIGNWLTQLYTLLIAENQFSGTIPEEIGN 61
           +GLNF+TSL+NS+ LK L+  TN  + QLP SIGN   QLY L ++ENQ SG +P+EIGN
Sbjct: 330 QGLNFVTSLSNSSLLKELTATTNLFSGQLPPSIGNLSAQLYLLYLSENQLSGNLPQEIGN 389

Query: 62  LVGLISVSFLTNSFTGNIPFPLENLKNLQILSLKSNFLSGFIPETFGNLSEPSQFTVNDN 121
           L GL  ++  +NSFTG IP  L NL++LQ L L +NFLSG IPE+ GNLS  S+  +N+N
Sbjct: 390 LGGLNVLTLDSNSFTGKIPSSLGNLRDLQALYLYTNFLSGSIPESLGNLSSLSEIGLNEN 449

Query: 122 NLGGKIPPSLTNCERLTIFYISENGLIGNLPDEIFNFKTLL--IFNVSFNNFTGHLPSQI 181
           NL G IP S +NC+R+ +F +S NGL G++P EIF+  T L  +FNVS N F+G LP +I
Sbjct: 450 NLSGGIPLSFSNCKRIKVFDVSANGLSGSIPKEIFSAYTTLGQLFNVSSNYFSGSLPDEI 509

Query: 182 DKLSMVQQFDVANN 194
            K+ MV+  DV+ N
Sbjct: 510 GKMKMVETLDVSRN 523

[Close]

BLAST of CSPI03G32100 vs. ExPASy TrEMBL
Match: A0A438DE81 (LRR receptor-like serine/threonine-protein kinase EFR OS=Vitis vinifera OX=29760 GN=EFR_18 PE=4 SV=1)

HSP 1 Score: 191.8 bits (486), Expect = 2.6e-45
Identity = 100/192 (52.08%), Postives = 136/192 (70.83%), Query Frame = 0


Query: 3   GLNFITSLTNSTSLKVLSVATNSLTCQLPRSIGNWLTQLYTLLIAENQFSGTIPEEIGNL 62
           GL+FITSL+NSTSL+V SVATN LT  LP SIGN  +QL  L++ +N F G IPE +GNL
Sbjct: 262 GLDFITSLSNSTSLRVFSVATNKLTGHLPSSIGNLSSQLALLVMGQNHFEGNIPEGVGNL 321

Query: 63  VGLISVSFLTNSFTGNIPFPLENLKNLQILSLKSNFLSGFIPETFGNLSEPSQFTVNDNN 122
             LI +S   N  TG+IP  + NL+NLQ L L SN+LSG IPE+ GNL++  +  ++ NN
Sbjct: 322 RSLIQLSMEENVLTGHIPSTIGNLQNLQSLILDSNYLSGSIPESLGNLTQLYELGLSGNN 381

Query: 123 LGGKIPPSLTNCERLTIFYISENGLIGNLPDEIFNFKTL-LIFNVSFNNFTGHLPSQIDK 182
           + G+IP SL++C+RL +  +S NGL  N+P EIF+F  L  + N+S+N+ +G LPS+I  
Sbjct: 382 ITGRIPSSLSSCQRLQLLDLSINGLRDNIPKEIFSFPNLATVLNLSWNSLSGSLPSEIGN 441

Query: 183 LSMVQQFDVANN 194
           L MVQ  D++NN
Sbjct: 442 LKMVQGIDISNN 453

[Close]

BLAST of CSPI03G32100 vs. NCBI nr
Match: KAE8651004.1 (hypothetical protein Csa_002076 [Cucumis sativus])

HSP 1 Score: 384.8 bits (987), Expect = 4.4e-103
Identity = 193/193 (100.00%), Postives = 193/193 (100.00%), Query Frame = 0


Query: 1   MEGLNFITSLTNSTSLKVLSVATNSLTCQLPRSIGNWLTQLYTLLIAENQFSGTIPEEIG 60
           MEGLNFITSLTNSTSLKVLSVATNSLTCQLPRSIGNWLTQLYTLLIAENQFSGTIPEEIG
Sbjct: 1   MEGLNFITSLTNSTSLKVLSVATNSLTCQLPRSIGNWLTQLYTLLIAENQFSGTIPEEIG 60

Query: 61  NLVGLISVSFLTNSFTGNIPFPLENLKNLQILSLKSNFLSGFIPETFGNLSEPSQFTVND 120
           NLVGLISVSFLTNSFTGNIPFPLENLKNLQILSLKSNFLSGFIPETFGNLSEPSQFTVND
Sbjct: 61  NLVGLISVSFLTNSFTGNIPFPLENLKNLQILSLKSNFLSGFIPETFGNLSEPSQFTVND 120

Query: 121 NNLGGKIPPSLTNCERLTIFYISENGLIGNLPDEIFNFKTLLIFNVSFNNFTGHLPSQID 180
           NNLGGKIPPSLTNCERLTIFYISENGLIGNLPDEIFNFKTLLIFNVSFNNFTGHLPSQID
Sbjct: 121 NNLGGKIPPSLTNCERLTIFYISENGLIGNLPDEIFNFKTLLIFNVSFNNFTGHLPSQID 180

Query: 181 KLSMVQQFDVANN 194
           KLSMVQQFDVANN
Sbjct: 181 KLSMVQQFDVANN 193

[Close]

BLAST of CSPI03G32100 vs. NCBI nr
Match: XP_022971300.1 (LRR receptor-like serine/threonine-protein kinase EFR [Cucurbita maxima])

HSP 1 Score: 268.1 bits (684), Expect = 6.0e-68
Identity = 143/192 (74.48%), Postives = 154/192 (80.21%), Query Frame = 0


Query: 2   EGLNFITSLTNSTSLKVLSVATNSLTCQLPRSIGNWLTQLYTLLIAENQFSGTIPEEIGN 61
           EGLNFITSLTNST LK+LSVATN LT QLPRSIGN       LL+AENQFSG+IP EIGN
Sbjct: 332 EGLNFITSLTNSTHLKMLSVATNLLTGQLPRSIGNLSNHCSKLLMAENQFSGSIPSEIGN 391

Query: 62  LVGLISVSFLTNSFTGNIPFPLENLKNLQILSLKSNFLSGFIPETFGNLSEPSQFTVNDN 121
           L GLISVS  +NSFTGNIP  L NLKNLQ+LSL+SN LSG IPETFGNLSE S F VNDN
Sbjct: 392 LGGLISVSLPSNSFTGNIPSSLSNLKNLQVLSLESNILSGSIPETFGNLSELSLFLVNDN 451

Query: 122 NLGGKIPPSLTNCERLTIFYISENGLIGNLPDEIFNFKTLLIFNVSFNNFTGHLPSQIDK 181
            L GKIP SLTNCERL +F +S+NGL GNLP EIF+F  LL FNVS NN TG LPS+IDK
Sbjct: 452 KLSGKIPLSLTNCERLLVFDLSQNGLSGNLPKEIFSFPNLLEFNVSVNNLTGSLPSEIDK 511

Query: 182 LSMVQQFDVANN 194
           L MVQ FDVA N
Sbjct: 512 LLMVQLFDVATN 523

[Close]

BLAST of CSPI03G32100 vs. NCBI nr
Match: XP_022960963.1 (LRR receptor-like serine/threonine-protein kinase EFR [Cucurbita moschata])

HSP 1 Score: 266.9 bits (681), Expect = 1.3e-67
Identity = 142/192 (73.96%), Postives = 154/192 (80.21%), Query Frame = 0


Query: 2   EGLNFITSLTNSTSLKVLSVATNSLTCQLPRSIGNWLTQLYTLLIAENQFSGTIPEEIGN 61
           EGLNFITSL NST LK+LSVATN LT QLPRSIGN    +  LL+AENQFSG+IP EIGN
Sbjct: 332 EGLNFITSLINSTHLKMLSVATNLLTGQLPRSIGNLSAHISKLLMAENQFSGSIPSEIGN 391

Query: 62  LVGLISVSFLTNSFTGNIPFPLENLKNLQILSLKSNFLSGFIPETFGNLSEPSQFTVNDN 121
           L GLISVS  +NSFTGNIP  L NLKNLQ+LSL+SN LSG IPETFGNLSE S F VNDN
Sbjct: 392 LGGLISVSLPSNSFTGNIPSSLSNLKNLQVLSLESNILSGSIPETFGNLSELSLFLVNDN 451

Query: 122 NLGGKIPPSLTNCERLTIFYISENGLIGNLPDEIFNFKTLLIFNVSFNNFTGHLPSQIDK 181
            L GKIP SLTNCERL +F +S+NGL GNLP EIF+F  LL FNVS NN TG LPS+IDK
Sbjct: 452 KLSGKIPLSLTNCERLLVFDLSQNGLSGNLPKEIFSFPNLLEFNVSVNNLTGSLPSEIDK 511

Query: 182 LSMVQQFDVANN 194
           L MVQ FDVA N
Sbjct: 512 LLMVQLFDVATN 523

[Close]

BLAST of CSPI03G32100 vs. NCBI nr
Match: KAG6596398.1 (LRR receptor-like serine/threonine-protein kinase EFR, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 266.5 bits (680), Expect = 1.7e-67
Identity = 142/192 (73.96%), Postives = 154/192 (80.21%), Query Frame = 0


Query: 2   EGLNFITSLTNSTSLKVLSVATNSLTCQLPRSIGNWLTQLYTLLIAENQFSGTIPEEIGN 61
           EGLNFITSL NST LK+LSVATN LT QLPRSIGN    +  LL+AENQFSG+IP EIGN
Sbjct: 332 EGLNFITSLINSTHLKMLSVATNLLTGQLPRSIGNLSAHISKLLMAENQFSGSIPSEIGN 391

Query: 62  LVGLISVSFLTNSFTGNIPFPLENLKNLQILSLKSNFLSGFIPETFGNLSEPSQFTVNDN 121
           L GLISVS  +NSFTGNIP  L NLKNLQ+LSL+SN LSG IPETFGNLSE S F VNDN
Sbjct: 392 LGGLISVSLPSNSFTGNIPSSLSNLKNLQVLSLESNILSGSIPETFGNLSELSLFLVNDN 451

Query: 122 NLGGKIPPSLTNCERLTIFYISENGLIGNLPDEIFNFKTLLIFNVSFNNFTGHLPSQIDK 181
            L GKIP SLTNCERL +F +S+NGL GNLP EIF+F  LL FNVS NN TG LPS+IDK
Sbjct: 452 KLSGKIPLSLTNCERLHVFDLSQNGLSGNLPKEIFSFPNLLEFNVSVNNLTGSLPSEIDK 511

Query: 182 LSMVQQFDVANN 194
           L MVQ FDVA N
Sbjct: 512 LLMVQLFDVATN 523

[Close]

BLAST of CSPI03G32100 vs. NCBI nr
Match: XP_023539541.1 (LRR receptor-like serine/threonine-protein kinase EFR [Cucurbita pepo subsp. pepo])

HSP 1 Score: 261.9 bits (668), Expect = 4.3e-66
Identity = 140/192 (72.92%), Postives = 152/192 (79.17%), Query Frame = 0


Query: 2   EGLNFITSLTNSTSLKVLSVATNSLTCQLPRSIGNWLTQLYTLLIAENQFSGTIPEEIGN 61
           EGLNFITSLTNST LK+LSVATN LT QLPRSIGN       LL+AENQFSG+IP EIGN
Sbjct: 332 EGLNFITSLTNSTHLKMLSVATNLLTGQLPRSIGNLSAHFSKLLMAENQFSGSIPSEIGN 391

Query: 62  LVGLISVSFLTNSFTGNIPFPLENLKNLQILSLKSNFLSGFIPETFGNLSEPSQFTVNDN 121
           L GLISVS  +NSF GNIP  L NLKNLQ+LSL+SN LSG IPE FGNLSE S F V+DN
Sbjct: 392 LGGLISVSLPSNSFMGNIPSSLSNLKNLQVLSLESNILSGSIPEPFGNLSELSLFLVSDN 451

Query: 122 NLGGKIPPSLTNCERLTIFYISENGLIGNLPDEIFNFKTLLIFNVSFNNFTGHLPSQIDK 181
            L GKIP SLTNCERL +F +S+NGL GNLP EIF+F  LL FNVS NN TG LPS+IDK
Sbjct: 452 KLSGKIPLSLTNCERLLVFDLSQNGLSGNLPKEIFSFPNLLEFNVSVNNLTGSLPSEIDK 511

Query: 182 LSMVQQFDVANN 194
           L MVQ FDVA N
Sbjct: 512 LLMVQLFDVATN 523

[Close]

BLAST of CSPI03G32100 vs. TAIR 10
Match: AT5G20480.1 (EF-TU receptor )

HSP 1 Score: 139.8 bits (351), Expect = 2.3e-33
Identity = 74/183 (40.44%), Postives = 104/183 (56.83%), Query Frame = 0


Query: 3   GLNFITSLTNSTSLKVLSVATNSLTCQLPRSIGNWLTQLYTLLIAENQFSGTIPEEIGNL 62
           GL FI ++ N T L+ L V  N L  +LP SI N  T L +L + +N  SGTIP +IGNL
Sbjct: 333 GLEFIGAVANCTQLEYLDVGYNRLGGELPASIANLSTTLTSLFLGQNLISGTIPHDIGNL 392

Query: 63  VGLISVSFLTNSFTGNIPFPLENLKNLQILSLKSNFLSGFIPETFGNLSEPSQFTVNDNN 122
           V L  +S  TN  +G +P     L NLQ++ L SN +SG IP  FGN++   +  +N N+
Sbjct: 393 VSLQELSLETNMLSGELPVSFGKLLNLQVVDLYSNAISGEIPSYFGNMTRLQKLHLNSNS 452

Query: 123 LGGKIPPSLTNCERLTIFYISENGLIGNLPDEIFNFKTLLIFNVSFNNFTGHLPSQIDKL 182
             G+IP SL  C  L   ++  N L G +P EI    +L   ++S N  TGH P ++ KL
Sbjct: 453 FHGRIPQSLGRCRYLLDLWMDTNRLNGTIPQEILQIPSLAYIDLSNNFLTGHFPEEVGKL 512

Query: 183 SMV 186
            ++
Sbjct: 513 ELL 515

[Close]

BLAST of CSPI03G32100 vs. TAIR 10
Match: AT3G47580.1 (Leucine-rich repeat protein kinase family protein )

HSP 1 Score: 130.6 bits (327), Expect = 1.4e-30
Identity = 75/190 (39.47%), Postives = 106/190 (55.79%), Query Frame = 0


Query: 4   LNFITSLTNSTSLKVLSVATNSLTCQLPRSIGNWLTQLYTLLIAENQFSGTIPEEIGNLV 63
           L FI SLTN T L++LSV    L   LP SI N  T+L +L +  N F G+IP++IGNL+
Sbjct: 327 LEFIDSLTNCTHLQLLSVGYTRLGGALPTSIANMSTELISLNLIGNHFFGSIPQDIGNLI 386

Query: 64  GLISVSFLTNSFTGNIPFPLENLKNLQILSLKSNFLSGFIPETFGNLSEPSQFTVNDNNL 123
           GL  +    N  TG +P  L  L  L +LSL SN +SG IP   GNL++     +++N+ 
Sbjct: 387 GLQRLQLGKNMLTGPLPTSLGKLLRLGLLSLYSNRMSGEIPSFIGNLTQLEILYLSNNSF 446

Query: 124 GGKIPPSLTNCERLTIFYISENGLIGNLPDEIFNFKTLLIFNVSFNNFTGHLPSQIDKLS 183
            G +PPSL  C  +    I  N L G +P EI    TL+  ++  N+ +G LP+ I  L 
Sbjct: 447 EGIVPPSLGKCSHMLDLRIGYNKLNGTIPKEIMQIPTLVNLSMEGNSLSGSLPNDIGSLQ 506

Query: 184 MVQQFDVANN 194
            + +  + NN
Sbjct: 507 NLVKLSLENN 516

[Close]

BLAST of CSPI03G32100 vs. TAIR 10
Match: AT1G35710.1 (Protein kinase family protein with leucine-rich repeat domain )

HSP 1 Score: 127.1 bits (318), Expect = 1.5e-29
Identity = 68/173 (39.31%), Postives = 101/173 (58.38%), Query Frame = 0


Query: 10  LTNSTSLKVLSVATNSLTCQLPRSIGNWLTQLYTLLIAENQFSGTIPEEIGNLVGLISVS 69
           L N  S+  L+++ N LT  +P ++GN L  L  L + EN  +G IP EIGN+  + +++
Sbjct: 218 LGNMESMTDLALSQNKLTGSIPSTLGN-LKNLMVLYLYENYLTGVIPPEIGNMESMTNLA 277

Query: 70  FLTNSFTGNIPFPLENLKNLQILSLKSNFLSGFIPETFGNLSEPSQFTVNDNNLGGKIPP 129
              N  TG+IP  L NLKNL +LSL  N+L+G IP   GN+       +++N L G IP 
Sbjct: 278 LSQNKLTGSIPSSLGNLKNLTLLSLFQNYLTGGIPPKLGNIESMIDLELSNNKLTGSIPS 337

Query: 130 SLTNCERLTIFYISENGLIGNLPDEIFNFKTLLIFNVSFNNFTGHLPSQIDKL 183
           SL N + LTI Y+ EN L G +P E+ N ++++   ++ N  TG +PS    L
Sbjct: 338 SLGNLKNLTILYLYENYLTGVIPPELGNMESMIDLQLNNNKLTGSIPSSFGNL 389

[Close]

BLAST of CSPI03G32100 vs. TAIR 10
Match: AT3G11080.1 (receptor like protein 35 )

HSP 1 Score: 126.3 bits (316), Expect = 2.6e-29
Identity = 72/186 (38.71%), Postives = 104/186 (55.91%), Query Frame = 0


Query: 8   TSLTNSTSLKVLSVATNSLTCQLPRSIGNWLTQLYTLLIAENQFSGTIPEEIGNLVGLIS 67
           +S+ N + L  L ++ N     +P SI N L++L +L ++ NQFSG IP  IGNL  L S
Sbjct: 143 SSIGNLSHLTSLHLSYNQFLGLIPSSIEN-LSRLTSLHLSSNQFSGQIPSSIGNLSHLTS 202

Query: 68  VSFLTNSFTGNIPFPLENLKNLQILSLKSNFLSGFIPETFGNLSEPSQFTVNDNNLGGKI 127
           +   +N F+G IP  + NL NL  LSL SN   G IP + GNL+  +   ++ NN  G+I
Sbjct: 203 LELSSNQFSGQIPSSIGNLSNLTFLSLPSNDFFGQIPSSIGNLARLTYLYLSYNNFVGEI 262

Query: 128 PPSLTNCERLTIFYISENGLIGNLPDEIFNFKTLLIFNVSFNNFTGHLPSQIDKLSMVQQ 187
           P S  N  +L +  +  N L GN+P  + N   L    +S N FTG +P+ I  LS +  
Sbjct: 263 PSSFGNLNQLIVLQVDSNKLSGNVPISLLNLTRLSALLLSHNQFTGTIPNNISLLSNLMD 322

Query: 188 FDVANN 194
           F+ +NN
Sbjct: 323 FEASNN 327

[Close]

BLAST of CSPI03G32100 vs. TAIR 10
Match: AT5G27060.1 (receptor like protein 53 )

HSP 1 Score: 124.8 bits (312), Expect = 7.6e-29
Identity = 72/187 (38.50%), Postives = 103/187 (55.08%), Query Frame = 0


Query: 7   ITSLTNSTSLKVLSVATNSLTCQLPRSIGNWLTQLYTLLIAENQFSGTIPEEIGNLVGLI 66
           + S+ N + L  L++  N  + Q P SI N L+ L  L ++ N+F G  P  IG L  L 
Sbjct: 162 LNSIGNLSRLTYLNLFDNQFSGQAPSSICN-LSHLTFLDLSYNRFFGQFPSSIGGLSHLT 221

Query: 67  SVSFLTNSFTGNIPFPLENLKNLQILSLKSNFLSGFIPETFGNLSEPSQFTVNDNNLGGK 126
           ++S  +N F+G IP  + NL NL  L L +N  SG IP   GNLS+ +   +  NN  G+
Sbjct: 222 TLSLFSNKFSGQIPSSIGNLSNLTTLDLSNNNFSGQIPSFIGNLSQLTFLGLFSNNFVGE 281

Query: 127 IPPSLTNCERLTIFYISENGLIGNLPDEIFNFKTLLIFNVSFNNFTGHLPSQIDKLSMVQ 186
           IP S  N  +LT  Y+ +N L GN P+ + N   L + ++S N FTG LP  I  LS + 
Sbjct: 282 IPSSFGNLNQLTRLYVDDNKLSGNFPNVLLNLTGLSLLSLSNNKFTGTLPPNITSLSNLM 341

Query: 187 QFDVANN 194
            FD ++N
Sbjct: 342 DFDASDN 347

The following BLAST results are available for this feature:

BLAST of CSPI03G32100 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
C0LGT6	3.2e-32	40.44	LRR receptor-like serine/threonine-protein kinase EFR OS=Arabidopsis thaliana OX...	[more]
Q1MX30	8.8e-30	39.68	Receptor kinase-like protein Xa21 OS=Oryza sativa subsp. indica OX=39946 GN=XA21...	[more]
Q2R2D5	2.0e-29	38.86	Receptor kinase-like protein Xa21 OS=Oryza sativa subsp. japonica OX=39947 GN=XA...	[more]
Q9LP24	2.2e-28	39.31	Probable leucine-rich repeat receptor-like protein kinase At1g35710 OS=Arabidops...	[more]
Q9SRL7	3.7e-28	38.71	Receptor-like protein 35 OS=Arabidopsis thaliana OX=3702 GN=RLP35 PE=3 SV=1	[more]

BLAST of CSPI03G32100 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LAH4	1.1e-99	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G734020 PE=4 SV=1	[more]
A0A6J1I5C7	2.9e-68	74.48	LRR receptor-like serine/threonine-protein kinase EFR OS=Cucurbita maxima OX=366...	[more]
A0A6J1HAI5	6.5e-68	73.96	LRR receptor-like serine/threonine-protein kinase EFR OS=Cucurbita moschata OX=3...	[more]
A0A6J1CXN3	1.3e-47	53.61	uncharacterized protein LOC111015147 OS=Momordica charantia OX=3673 GN=LOC111015...	[more]
A0A438DE81	2.6e-45	52.08	LRR receptor-like serine/threonine-protein kinase EFR OS=Vitis vinifera OX=29760...	[more]

BLAST of CSPI03G32100 vs. NCBI nr


Match Name	E-value	Identity	Description
KAE8651004.1	4.4e-103	100.00	hypothetical protein Csa_002076 [Cucumis sativus]	[more]
XP_022971300.1	6.0e-68	74.48	LRR receptor-like serine/threonine-protein kinase EFR [Cucurbita maxima]	[more]
XP_022960963.1	1.3e-67	73.96	LRR receptor-like serine/threonine-protein kinase EFR [Cucurbita moschata]	[more]
KAG6596398.1	1.7e-67	73.96	LRR receptor-like serine/threonine-protein kinase EFR, partial [Cucurbita argyro...	[more]
XP_023539541.1	4.3e-66	72.92	LRR receptor-like serine/threonine-protein kinase EFR [Cucurbita pepo subsp. pep...	[more]

BLAST of CSPI03G32100 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G20480.1	2.3e-33	40.44	EF-TU receptor	[more]
AT3G47580.1	1.4e-30	39.47	Leucine-rich repeat protein kinase family protein	[more]
AT1G35710.1	1.5e-29	39.31	Protein kinase family protein with leucine-rich repeat domain	[more]
AT3G11080.1	2.6e-29	38.71	receptor like protein 35	[more]
AT5G27060.1	7.6e-29	38.50	receptor like protein 53	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR032675	Leucine-rich repeat domain superfamily	GENE3D	3.80.10.10	Ribonuclease Inhibitor	coord: 3..193 e-value: 1.1E-44 score: 154.7
IPR001611	Leucine-rich repeat	PFAM	PF00560	LRR_1	coord: 88..110 e-value: 0.083 score: 13.5
None	No IPR available	PANTHER	PTHR48054	RECEPTOR KINASE-LIKE PROTEIN XA21	coord: 40..180 coord: 9..128
None	No IPR available	PANTHER	PTHR48054:SF10	LRR RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE RPK2	coord: 40..180 coord: 9..128
None	No IPR available	SUPERFAMILY	52058	L domain-like	coord: 8..193

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G32100.1	CSPI03G32100.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006468	protein phosphorylation
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0005515	protein binding
molecular_function	GO:0004672	protein kinase activity