CSPI03G32040 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI03G32040
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	tropinone reductase homolog
Location	Chr3: 28742944 .. 28744454 (-)
RNA-Seq Expression	CSPI03G32040
Synteny	CSPI03G32040

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GAATTGTTTATATATTTATATGTTATCCTAAGTTGATGAATTATAAAAGAAAAAATGAATCTCGTGGTTCGGACCTCACCATAATAATTGAATATTTACATATTAAAATGATGCAATTTTTAGTTCATATAATTAAGTTAAGAAAAATCGAGCGAGAGTGATCTCTCTCTTCCTTCATTTGTTGTGAATCAAATCGAAAGTCGAAACCCAACCCAGCTGCCATGGCCAACATTAGAAACCAAAGATGGTCACTCAAGGGGATGACCGCTCTCGTCACCGGCGGAAGCCGCGGCATCGGGTACTCCTAATTTTCAGTCTTTATTTTCGTTACGCCGATTCATCGAACTCCTGTAATGTTTTTTCTTTCTTCAGACGAGCCACGGTGGAAGAACTTGCAGAATTCGGGGCCAGGGTTCACACATGCTGTCGAAGCCAAGAAGATTTAGACAAATGTTTGAAAGAATGGGAAGCAATGGGCTTCAAAGTGAGTGGCTCTGTATGCGATGTCCAAAGCAAAGAACAGAGGAAGAAGCTGATGGAAACCGTTTCCTCACTCTTCAATGGGACCCTAAACATTCTGGTAATTAACTCCCAAGAAAAAAAAAACGAAAGTTTTGATTTTTGGATTTTGGATTTTGGATTTTGGATTTTGATCGATTATTTGAGTTAGGTGAATAATGCTGGGAGAACGTTGTCGTCGTTGAAGTCGACGGTAGAAGTAACAGAGGAAGATATTTCAAGTGTAATGAGTACAAATTTTGAGTCGTCGTTCCATTTTAGCCAACTTGCATATCCATTACTAAAGGCTTCTGGTAATGGAAGCATTGTGTTCATATCCTCTGTTTCAGGCTTAACGGCCCTTCCTTTCTCTACTCCATATGCTGCTTCTAAAGGTAACCCACAAATTTCTTCACCTTTACCCACCTTATTTTGAATTTGAATTTGTGTTCTTATAACATGAATTTGTGTTCTTATAACATGAATTTGTTGATGAAGCTGCCATAAATCAAATCACAAAGAACTTGGCATGTGAATGGGCAAAAGATAACATCCGTACAAACGCAGTGGCTCCATGGATCATCAAAACTCGCCTTGTCGAGCGTTCCAATGTACAAACTTTCATTCATTATCGATATCTCTTTCAATTCGATCCAAGTTTATTAATTATTTTTTTAACTTTTCTATTATTTTTCAGGATGATCCAATGCATGTAAAAGGAATAGAGCAACTATTGTCAGTAACGCCTCTTAAACGGGCAGGGGAACCACATGAAGTTTCGTCAATGGTTGTTTTCCTTTGTCTTCCAGCTGCGTCTTATATTACTGGACAACTTTTTGTTATTGATGGAGGCCACACTGTCAAGGCTTACCCCATACCCGATCTCTGAAACTAAACAAAACTAGTCTTTGTATTTCTCTATGTAATTTTAATCAATTTTAAAATTAGTATGGACTTGAATCTTTAGACTATATACAATCAATCTAATTTCCAATCACTCATCGTACACCAAG

mRNA sequence

GAATTGTTTATATATTTATATGTTATCCTAAGTTGATGAATTATAAAAGAAAAAATGAATCTCGTGGTTCGGACCTCACCATAATAATTGAATATTTACATATTAAAATGATGCAATTTTTAGTTCATATAATTAAGTTAAGAAAAATCGAGCGAGAGTGATCTCTCTCTTCCTTCATTTGTTGTGAATCAAATCGAAAGTCGAAACCCAACCCAGCTGCCATGGCCAACATTAGAAACCAAAGATGGTCACTCAAGGGGATGACCGCTCTCGTCACCGGCGGAAGCCGCGGCATCGGACGAGCCACGGTGGAAGAACTTGCAGAATTCGGGGCCAGGGTTCACACATGCTGTCGAAGCCAAGAAGATTTAGACAAATGTTTGAAAGAATGGGAAGCAATGGGCTTCAAAGTGAGTGGCTCTGTATGCGATGTCCAAAGCAAAGAACAGAGGAAGAAGCTGATGGAAACCGTTTCCTCACTCTTCAATGGGACCCTAAACATTCTGGTGAATAATGCTGGGAGAACGTTGTCGTCGTTGAAGTCGACGGTAGAAGTAACAGAGGAAGATATTTCAAGTGTAATGAGTACAAATTTTGAGTCGTCGTTCCATTTTAGCCAACTTGCATATCCATTACTAAAGGCTTCTGGTAATGGAAGCATTGTGTTCATATCCTCTGTTTCAGGCTTAACGGCCCTTCCTTTCTCTACTCCATATGCTGCTTCTAAAGCTGCCATAAATCAAATCACAAAGAACTTGGCATGTGAATGGGCAAAAGATAACATCCGTACAAACGCAGTGGCTCCATGGATCATCAAAACTCGCCTTGTCGAGCGTTCCAATGATGATCCAATGCATGTAAAAGGAATAGAGCAACTATTGTCAGTAACGCCTCTTAAACGGGCAGGGGAACCACATGAAGTTTCGTCAATGGTTGTTTTCCTTTGTCTTCCAGCTGCGTCTTATATTACTGGACAACTTTTTGTTATTGATGGAGGCCACACTGTCAAGGCTTACCCCATACCCGATCTCTGAAACTAAACAAAACTAGTCTTTGTATTTCTCTATGTAATTTTAATCAATTTTAAAATTAGTATGGACTTGAATCTTTAGACTATATACAATCAATCTAATTTCCAATCACTCATCGTACACCAAG

Coding sequence (CDS)

ATGGCCAACATTAGAAACCAAAGATGGTCACTCAAGGGGATGACCGCTCTCGTCACCGGCGGAAGCCGCGGCATCGGACGAGCCACGGTGGAAGAACTTGCAGAATTCGGGGCCAGGGTTCACACATGCTGTCGAAGCCAAGAAGATTTAGACAAATGTTTGAAAGAATGGGAAGCAATGGGCTTCAAAGTGAGTGGCTCTGTATGCGATGTCCAAAGCAAAGAACAGAGGAAGAAGCTGATGGAAACCGTTTCCTCACTCTTCAATGGGACCCTAAACATTCTGGTGAATAATGCTGGGAGAACGTTGTCGTCGTTGAAGTCGACGGTAGAAGTAACAGAGGAAGATATTTCAAGTGTAATGAGTACAAATTTTGAGTCGTCGTTCCATTTTAGCCAACTTGCATATCCATTACTAAAGGCTTCTGGTAATGGAAGCATTGTGTTCATATCCTCTGTTTCAGGCTTAACGGCCCTTCCTTTCTCTACTCCATATGCTGCTTCTAAAGCTGCCATAAATCAAATCACAAAGAACTTGGCATGTGAATGGGCAAAAGATAACATCCGTACAAACGCAGTGGCTCCATGGATCATCAAAACTCGCCTTGTCGAGCGTTCCAATGATGATCCAATGCATGTAAAAGGAATAGAGCAACTATTGTCAGTAACGCCTCTTAAACGGGCAGGGGAACCACATGAAGTTTCGTCAATGGTTGTTTTCCTTTGTCTTCCAGCTGCGTCTTATATTACTGGACAACTTTTTGTTATTGATGGAGGCCACACTGTCAAGGCTTACCCCATACCCGATCTCTGA

Protein sequence

MANIRNQRWSLKGMTALVTGGSRGIGRATVEELAEFGARVHTCCRSQEDLDKCLKEWEAMGFKVSGSVCDVQSKEQRKKLMETVSSLFNGTLNILVNNAGRTLSSLKSTVEVTEEDISSVMSTNFESSFHFSQLAYPLLKASGNGSIVFISSVSGLTALPFSTPYAASKAAINQITKNLACEWAKDNIRTNAVAPWIIKTRLVERSNDDPMHVKGIEQLLSVTPLKRAGEPHEVSSMVVFLCLPAASYITGQLFVIDGGHTVKAYPIPDL*

Homology

[Close]

BLAST of CSPI03G32040 vs. ExPASy Swiss-Prot
Match: P50165 (Tropinone reductase homolog OS=Datura stramonium OX=4076 PE=2 SV=1)

HSP 1 Score: 330.5 bits (846), Expect = 1.8e-89
Identity = 164/260 (63.08%), Postives = 200/260 (76.92%), Query Frame = 0


Query: 6   NQRWSLKGMTALVTGGSRGIGRATVEELAEFGARVHTCCRSQEDLDKCLKEWEAMGFKVS 65
           ++RWSL+GMTALVTGG+RGIG A VEELA FGA V+TC RSQ DLD+CL++W   GFKVS
Sbjct: 10  DRRWSLRGMTALVTGGTRGIGYAIVEELANFGAEVYTCSRSQNDLDECLEKWRRKGFKVS 69

Query: 66  GSVCDVQSKEQRKKLMETVSSLFNGTLNILVNNAGRTLSSLKSTVEVTEEDISSVMSTNF 125
           G VCDV S  QR+ LME+V+S FNG LNIL+NNAG T+   K     T ED S +M TNF
Sbjct: 70  GPVCDVSSISQRQTLMESVTSSFNGKLNILINNAGTTIP--KEATNFTAEDYSIIMGTNF 129

Query: 126 ESSFHFSQLAYPLLKASGNGSIVFISSVSGLTALPFSTPYAASKAAINQITKNLACEWAK 185
           E+S++  QLA+PLLKASGN SIVF SS +G+ A+P S+ YAASK AINQ+TK+LACEWAK
Sbjct: 130 EASYNLCQLAHPLLKASGNASIVFNSSAAGVIAVPLSSIYAASKGAINQVTKSLACEWAK 189

Query: 186 DNIRTNAVAPWIIKTRLVERSNDDPMHVKGIEQLLSVTPLKRAGEPHEVSSMVVFLCLPA 245
           D+IR NAVAPWII T ++E +   P   K IE L+   P+KRAGEP EVSS+V +LCLP 
Sbjct: 190 DSIRVNAVAPWIINTPIIEAACQVPSQKKNIESLIGRAPMKRAGEPSEVSSLVTYLCLPT 249

Query: 246 ASYITGQLFVIDGGHTVKAY 266
           ASYITGQ+  +DGG+TV  +
Sbjct: 250 ASYITGQIICVDGGYTVNGF 267

[Close]

BLAST of CSPI03G32040 vs. ExPASy Swiss-Prot
Match: Q9LHT0 (Tropinone reductase homolog At5g06060 OS=Arabidopsis thaliana OX=3702 GN=At5g06060 PE=2 SV=1)

HSP 1 Score: 311.6 bits (797), Expect = 8.7e-84
Identity = 157/260 (60.38%), Postives = 198/260 (76.15%), Query Frame = 0


Query: 6   NQRWSLKGMTALVTGGSRGIGRATVEELAEFGARVHTCCRSQEDLDKCLKEWEAMGFKVS 65
           ++RWSL G TALVTGG+RGIGRA VEELA+FGA+VHTC R+QE+L+ CL +W+A G  VS
Sbjct: 4   DKRWSLAGKTALVTGGTRGIGRAVVEELAKFGAKVHTCSRNQEELNACLNDWKANGLVVS 63

Query: 66  GSVCDVQSKEQRKKLMETVSSLFNGTLNILVNNAGRTLSSLKSTVEVTEEDISSVMSTNF 125
           GSVCD   ++QR+KL++  SS F+G LNIL+NN G  +   K TVE + E+ + +MSTN 
Sbjct: 64  GSVCDASVRDQREKLIQEASSAFSGKLNILINNVGTNVR--KPTVEYSSEEYAKIMSTNL 123

Query: 126 ESSFHFSQLAYPLLKASGNGSIVFISSVSGLTALPFSTPYAASKAAINQITKNLACEWAK 185
           ES+FH SQ+A+PLLKASG GSIVFISSV+GL  L   + Y A+K A+NQ+T+NLACEWA 
Sbjct: 124 ESAFHLSQIAHPLLKASGVGSIVFISSVAGLVHLSSGSIYGATKGALNQLTRNLACEWAS 183

Query: 186 DNIRTNAVAPWIIKTRLVERSNDDPMHVKGIEQLLSVTPLKRAGEPHEVSSMVVFLCLPA 245
           DNIRTN VAPW IKT LVE   +     + +E ++S TPL R GEP EVSS+V FLCLPA
Sbjct: 184 DNIRTNCVAPWYIKTSLVETLLEKK---EFVEAVVSRTPLGRVGEPEEVSSLVAFLCLPA 243

Query: 246 ASYITGQLFVIDGGHTVKAY 266
           +SYITGQ+  +DGG TV  +
Sbjct: 244 SSYITGQVISVDGGFTVNGF 258

[Close]

BLAST of CSPI03G32040 vs. ExPASy Swiss-Prot
Match: Q9ZW12 (Tropinone reductase homolog At2g29260, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At2g29260 PE=2 SV=1)

HSP 1 Score: 301.2 bits (770), Expect = 1.2e-80
Identity = 152/259 (58.69%), Postives = 193/259 (74.52%), Query Frame = 0


Query: 7   QRWSLKGMTALVTGGSRGIGRATVEELAEFGARVHTCCRSQEDLDKCLKEWEAMGFKVSG 66
           +RWSL GM+ALVTGG+RGIGRA VEELA  GA VHTC R++ +L+ CL +W   GF+V+G
Sbjct: 64  ERWSLNGMSALVTGGTRGIGRAIVEELAGLGAEVHTCARNEYELENCLSDWNRSGFRVAG 123

Query: 67  SVCDVQSKEQRKKLMETVSSLFNGTLNILVNNAGRTLSSLKSTVEVTEEDISSVMSTNFE 126
           SVCDV  + QR+ LMETVSS+F G L+ILVNN G  +   K  VE T  + S++MSTNFE
Sbjct: 124 SVCDVSDRSQREALMETVSSVFEGKLHILVNNVGTNIR--KPMVEFTAGEFSTLMSTNFE 183

Query: 127 SSFHFSQLAYPLLKASGNGSIVFISSVSGLTALPFSTPYAASKAAINQITKNLACEWAKD 186
           S FH  QLAYPLL+ S  GS+VFISSVSG  +L   +  +++K AINQ+T++LACEWAKD
Sbjct: 184 SVFHLCQLAYPLLRESKAGSVVFISSVSGFVSLKNMSVQSSTKGAINQLTRSLACEWAKD 243

Query: 187 NIRTNAVAPWIIKTRLVERSNDDPMHVKGIEQLLSVTPLKRAGEPHEVSSMVVFLCLPAA 246
           NIR NAVAPW IKT +VE+   +  +   +E++ SVTPL R GEP EVSS V FLCLPA+
Sbjct: 244 NIRINAVAPWYIKTSMVEQVLSNKEY---LEEVYSVTPLGRLGEPREVSSAVAFLCLPAS 303

Query: 247 SYITGQLFVIDGGHTVKAY 266
           SYITGQ+  +DGG ++  +
Sbjct: 304 SYITGQILCVDGGMSINGF 317

[Close]

BLAST of CSPI03G32040 vs. ExPASy Swiss-Prot
Match: Q9ZW20 (Tropinone reductase homolog At2g29370 OS=Arabidopsis thaliana OX=3702 GN=At2g29370 PE=3 SV=1)

HSP 1 Score: 297.7 bits (761), Expect = 1.3e-79
Identity = 152/262 (58.02%), Postives = 197/262 (75.19%), Query Frame = 0


Query: 8   RWSLKGMTALVTGGSRGIGRATVEELAEFGARVHTCCRSQEDLDKCLKEWEAMGFKVSGS 67
           +WSL+GMTALVTGGS+G+G+A VEELA  GARVHTC R +  L + L+EW+A G +V+ S
Sbjct: 13  KWSLEGMTALVTGGSKGLGKAVVEELAMLGARVHTCARDETQLQESLREWQAKGLQVTTS 72

Query: 68  VCDVQSKEQRKKLMETVSSLFNGTLNILVNNAGRTLSSLKSTVEVTEEDISSVMSTNFES 127
           VCDV S++QR+KLMETVSSLF G L+ILV N G  +  LK T E T E+ S +++TN ES
Sbjct: 73  VCDVSSRDQREKLMETVSSLFQGKLSILVPNVG--IGVLKPTTECTAEEFSFIIATNLES 132

Query: 128 SFHFSQLAYPLLKASGNGSIVFISSVSGLTALPFSTPYAASKAAINQITKNLACEWAKDN 187
           +FHFSQLA+PLLKASG+G+IV +SSV+G+  L  ++ Y A+K A+NQ+ +NLACEWA DN
Sbjct: 133 TFHFSQLAHPLLKASGSGNIVLMSSVAGVVNLGNTSIYGATKGAMNQLARNLACEWASDN 192

Query: 188 IRTNAVAPWIIKTRLVERSNDDPMHVKGI-EQLLSVTPLKRAGEPHEVSSMVVFLCLPAA 247
           IR N+V PW I T     S  D +  K + E++ SVTPL+R GE +EVSS+V FLCLPAA
Sbjct: 193 IRANSVCPWFITT----PSTKDFLGDKDVKEKVESVTPLRRVGEANEVSSLVAFLCLPAA 252

Query: 248 SYITGQLFVIDGGHTVKAYPIP 269
           SYITGQ   +DGG T+  + +P
Sbjct: 253 SYITGQTICVDGGFTINGFSLP 268

[Close]

BLAST of CSPI03G32040 vs. ExPASy Swiss-Prot
Match: Q9ZW19 (Tropinone reductase homolog At2g29360 OS=Arabidopsis thaliana OX=3702 GN=SDR PE=1 SV=1)

HSP 1 Score: 296.6 bits (758), Expect = 2.9e-79
Identity = 154/264 (58.33%), Postives = 194/264 (73.48%), Query Frame = 0


Query: 8   RWSLKGMTALVTGGSRGIGRATVEELAEFGARVHTCCRSQEDLDKCLKEWEAMGFKVSGS 67
           RWSL GMTALVTGGS+GIG A VEELA  GAR+HTC R +  L + L++W+A GF+V+ S
Sbjct: 13  RWSLVGMTALVTGGSKGIGEAVVEELATLGARIHTCARDETQLQESLRKWQAKGFQVTTS 72

Query: 68  VCDVQSKEQRKKLMETVSSLFNGTLNILVNNAGRTLSSLKSTVEVTEEDISSVMSTNFES 127
           VCDV S+++R+KLMETVS++F G LNILVNN G  +  +K T++ T ED S  M+TN ES
Sbjct: 73  VCDVSSRDKREKLMETVSTIFEGKLNILVNNVGTCI--VKPTLQHTAEDFSFTMATNLES 132

Query: 128 SFHFSQLAYPLLKASGNGSIVFISSVSGLTALPFSTPYAASKAAINQITKNLACEWAKDN 187
           +FH SQLA+PLLKASG+GSIV ISSVSG+  +  ++ Y  SK A+NQ+ +NLACEWA DN
Sbjct: 133 AFHLSQLAHPLLKASGSGSIVLISSVSGVVHVNGASIYGVSKGAMNQLGRNLACEWASDN 192

Query: 188 IRTNAVAPWIIKTRLVERSNDDPMHVKGIEQLLSVTPLKRAGEPHEVSSMVVFLCLPAAS 247
           IRTN+V PW I+T LV  S  +    K +E   S  P+ R GE +EVSS+V FLCLPAAS
Sbjct: 193 IRTNSVCPWFIETPLVTESLSNEEFRKEVE---SRPPMGRVGEVNEVSSLVAFLCLPAAS 252

Query: 248 YITGQLFVIDGGHTVKAY---PIP 269
           YITGQ   +DGG TV  +   P+P
Sbjct: 253 YITGQTICVDGGFTVNGFSFKPLP 271

[Close]

BLAST of CSPI03G32040 vs. ExPASy TrEMBL
Match: A0A0A0LDM3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G733960 PE=4 SV=1)

HSP 1 Score: 533.9 bits (1374), Expect = 4.0e-148
Identity = 270/270 (100.00%), Postives = 270/270 (100.00%), Query Frame = 0


Query: 1   MANIRNQRWSLKGMTALVTGGSRGIGRATVEELAEFGARVHTCCRSQEDLDKCLKEWEAM 60
           MANIRNQRWSLKGMTALVTGGSRGIGRATVEELAEFGARVHTCCRSQEDLDKCLKEWEAM
Sbjct: 1   MANIRNQRWSLKGMTALVTGGSRGIGRATVEELAEFGARVHTCCRSQEDLDKCLKEWEAM 60

Query: 61  GFKVSGSVCDVQSKEQRKKLMETVSSLFNGTLNILVNNAGRTLSSLKSTVEVTEEDISSV 120
           GFKVSGSVCDVQSKEQRKKLMETVSSLFNGTLNILVNNAGRTLSSLKSTVEVTEEDISSV
Sbjct: 61  GFKVSGSVCDVQSKEQRKKLMETVSSLFNGTLNILVNNAGRTLSSLKSTVEVTEEDISSV 120

Query: 121 MSTNFESSFHFSQLAYPLLKASGNGSIVFISSVSGLTALPFSTPYAASKAAINQITKNLA 180
           MSTNFESSFHFSQLAYPLLKASGNGSIVFISSVSGLTALPFSTPYAASKAAINQITKNLA
Sbjct: 121 MSTNFESSFHFSQLAYPLLKASGNGSIVFISSVSGLTALPFSTPYAASKAAINQITKNLA 180

Query: 181 CEWAKDNIRTNAVAPWIIKTRLVERSNDDPMHVKGIEQLLSVTPLKRAGEPHEVSSMVVF 240
           CEWAKDNIRTNAVAPWIIKTRLVERSNDDPMHVKGIEQLLSVTPLKRAGEPHEVSSMVVF
Sbjct: 181 CEWAKDNIRTNAVAPWIIKTRLVERSNDDPMHVKGIEQLLSVTPLKRAGEPHEVSSMVVF 240

Query: 241 LCLPAASYITGQLFVIDGGHTVKAYPIPDL 271
           LCLPAASYITGQLFVIDGGHTVKAYPIPDL
Sbjct: 241 LCLPAASYITGQLFVIDGGHTVKAYPIPDL 270

[Close]

BLAST of CSPI03G32040 vs. ExPASy TrEMBL
Match: A0A5D3DN54 (Tropinone reductase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G001430 PE=4 SV=1)

HSP 1 Score: 509.2 bits (1310), Expect = 1.0e-140
Identity = 256/270 (94.81%), Postives = 262/270 (97.04%), Query Frame = 0


Query: 1   MANIRNQRWSLKGMTALVTGGSRGIGRATVEELAEFGARVHTCCRSQEDLDKCLKEWEAM 60
           MANIRNQRWSLKG TALVTGGSRGIGRATVEELAEFGARVHTCCRSQEDLDKCLKEWE M
Sbjct: 1   MANIRNQRWSLKGKTALVTGGSRGIGRATVEELAEFGARVHTCCRSQEDLDKCLKEWEEM 60

Query: 61  GFKVSGSVCDVQSKEQRKKLMETVSSLFNGTLNILVNNAGRTLSSLKSTVEVTEEDISSV 120
           GF+VSGSVCDVQSKEQRKKLMETVSSLFNGTLNILVNNAGRTLSSLKSTVEVTEE+ISSV
Sbjct: 61  GFEVSGSVCDVQSKEQRKKLMETVSSLFNGTLNILVNNAGRTLSSLKSTVEVTEEEISSV 120

Query: 121 MSTNFESSFHFSQLAYPLLKASGNGSIVFISSVSGLTALPFSTPYAASKAAINQITKNLA 180
           MSTNFE+S HFSQLAYPLLKASGNGSIVFISSVSGL ALPFSTPYAASKAAINQ+TKNLA
Sbjct: 121 MSTNFEASLHFSQLAYPLLKASGNGSIVFISSVSGLMALPFSTPYAASKAAINQMTKNLA 180

Query: 181 CEWAKDNIRTNAVAPWIIKTRLVERSNDDPMHVKGIEQLLSVTPLKRAGEPHEVSSMVVF 240
           CEWAKDNIRTNAVAPWIIKTR VE SNDDPMHVKG+EQ+LSVTPLKRAGEPHEVSSMV F
Sbjct: 181 CEWAKDNIRTNAVAPWIIKTRFVESSNDDPMHVKGMEQILSVTPLKRAGEPHEVSSMVAF 240

Query: 241 LCLPAASYITGQLFVIDGGHTVKAYPIPDL 271
           LCLPAASYITGQLFVIDGGHTVKAYPI DL
Sbjct: 241 LCLPAASYITGQLFVIDGGHTVKAYPISDL 270

[Close]

BLAST of CSPI03G32040 vs. ExPASy TrEMBL
Match: A0A1S3B5V0 (tropinone reductase homolog OS=Cucumis melo OX=3656 GN=LOC103486361 PE=4 SV=1)

HSP 1 Score: 509.2 bits (1310), Expect = 1.0e-140
Identity = 256/270 (94.81%), Postives = 262/270 (97.04%), Query Frame = 0


Query: 1   MANIRNQRWSLKGMTALVTGGSRGIGRATVEELAEFGARVHTCCRSQEDLDKCLKEWEAM 60
           MANIRNQRWSLKG TALVTGGSRGIGRATVEELAEFGARVHTCCRSQEDLDKCLKEWE M
Sbjct: 1   MANIRNQRWSLKGKTALVTGGSRGIGRATVEELAEFGARVHTCCRSQEDLDKCLKEWEEM 60

Query: 61  GFKVSGSVCDVQSKEQRKKLMETVSSLFNGTLNILVNNAGRTLSSLKSTVEVTEEDISSV 120
           GF+VSGSVCDVQSKEQRKKLMETVSSLFNGTLNILVNNAGRTLSSLKSTVEVTEE+ISSV
Sbjct: 61  GFEVSGSVCDVQSKEQRKKLMETVSSLFNGTLNILVNNAGRTLSSLKSTVEVTEEEISSV 120

Query: 121 MSTNFESSFHFSQLAYPLLKASGNGSIVFISSVSGLTALPFSTPYAASKAAINQITKNLA 180
           MSTNFE+S HFSQLAYPLLKASGNGSIVFISSVSGL ALPFSTPYAASKAAINQ+TKNLA
Sbjct: 121 MSTNFEASLHFSQLAYPLLKASGNGSIVFISSVSGLMALPFSTPYAASKAAINQMTKNLA 180

Query: 181 CEWAKDNIRTNAVAPWIIKTRLVERSNDDPMHVKGIEQLLSVTPLKRAGEPHEVSSMVVF 240
           CEWAKDNIRTNAVAPWIIKTR VE SNDDPMHVKG+EQ+LSVTPLKRAGEPHEVSSMV F
Sbjct: 181 CEWAKDNIRTNAVAPWIIKTRFVESSNDDPMHVKGMEQILSVTPLKRAGEPHEVSSMVAF 240

Query: 241 LCLPAASYITGQLFVIDGGHTVKAYPIPDL 271
           LCLPAASYITGQLFVIDGGHTVKAYPI DL
Sbjct: 241 LCLPAASYITGQLFVIDGGHTVKAYPISDL 270

[Close]

BLAST of CSPI03G32040 vs. ExPASy TrEMBL
Match: A0A6J1I667 (tropinone reductase homolog OS=Cucurbita maxima OX=3661 GN=LOC111470275 PE=4 SV=1)

HSP 1 Score: 432.2 bits (1110), Expect = 1.6e-117
Identity = 217/270 (80.37%), Postives = 244/270 (90.37%), Query Frame = 0


Query: 1   MANI-RNQRWSLKGMTALVTGGSRGIGRATVEELAEFGARVHTCCRSQEDLDKCLKEWEA 60
           MAN  R+QRWSL+GMTALVTGG+ GIGRATVEELA FGA VHTC RSQ DLDKCLKEWE 
Sbjct: 1   MANTSRDQRWSLQGMTALVTGGTHGIGRATVEELAAFGAIVHTCSRSQGDLDKCLKEWEE 60

Query: 61  MGFKVSGSVCDVQSKEQRKKLMETVSSLFNGTLNILVNNAGRTLSSLKSTVEVTEEDISS 120
           MG  VSGSVCDV S+EQRK+LMETVSSLFNG+LNILVNNAG   S+ KS++EVTEE++SS
Sbjct: 61  MGLNVSGSVCDVHSREQRKRLMETVSSLFNGSLNILVNNAG--TSAYKSSLEVTEEELSS 120

Query: 121 VMSTNFESSFHFSQLAYPLLKASGNGSIVFISSVSGLTALPFSTPYAASKAAINQITKNL 180
           VMSTNFE+SFHFSQLA+PL+KASGNGSIVF+SSV+GL +LP+STPYAASKAAINQIT+NL
Sbjct: 121 VMSTNFEASFHFSQLAHPLMKASGNGSIVFMSSVAGLMSLPYSTPYAASKAAINQITRNL 180

Query: 181 ACEWAKDNIRTNAVAPWIIKTRLVERSNDDPMHVKGIEQLLSVTPLKRAGEPHEVSSMVV 240
           ACEWAKDNIRTNAVAPWII+TR V+   DDP+HV+ +  +LSVTPLKRAGEPHEVSSMV 
Sbjct: 181 ACEWAKDNIRTNAVAPWIIRTRFVKPPTDDPIHVEVMNHMLSVTPLKRAGEPHEVSSMVA 240

Query: 241 FLCLPAASYITGQLFVIDGGHTVKAYPIPD 270
           FLCLPAASYITGQ+F IDGGHTV A+PIPD
Sbjct: 241 FLCLPAASYITGQVFCIDGGHTVTAFPIPD 268

[Close]

BLAST of CSPI03G32040 vs. ExPASy TrEMBL
Match: A0A6J1FKX7 (tropinone reductase homolog OS=Cucurbita moschata OX=3662 GN=LOC111446681 PE=4 SV=1)

HSP 1 Score: 431.8 bits (1109), Expect = 2.1e-117
Identity = 215/270 (79.63%), Postives = 245/270 (90.74%), Query Frame = 0


Query: 1   MANI-RNQRWSLKGMTALVTGGSRGIGRATVEELAEFGARVHTCCRSQEDLDKCLKEWEA 60
           MAN  R+QRWSL+GMTALVTGG+ GIGRATVEELA FGA VHTC RSQ DLDKCLKEWE 
Sbjct: 1   MANTSRDQRWSLQGMTALVTGGTHGIGRATVEELAAFGATVHTCSRSQGDLDKCLKEWEE 60

Query: 61  MGFKVSGSVCDVQSKEQRKKLMETVSSLFNGTLNILVNNAGRTLSSLKSTVEVTEEDISS 120
           MGF VSGSVCDV S+EQR +L+ETVSSLFNG+LNILVNNAG   S+ KS++EVTEE++SS
Sbjct: 61  MGFNVSGSVCDVHSREQRMQLIETVSSLFNGSLNILVNNAG--TSAYKSSLEVTEEELSS 120

Query: 121 VMSTNFESSFHFSQLAYPLLKASGNGSIVFISSVSGLTALPFSTPYAASKAAINQITKNL 180
           VMSTNFE++FHFSQLA+PL+KASGNGSIVF+SSV+GL +LP+STPYAASKAAINQIT+NL
Sbjct: 121 VMSTNFEAAFHFSQLAHPLMKASGNGSIVFMSSVAGLMSLPYSTPYAASKAAINQITRNL 180

Query: 181 ACEWAKDNIRTNAVAPWIIKTRLVERSNDDPMHVKGIEQLLSVTPLKRAGEPHEVSSMVV 240
           ACEWAKDNIRTNAVAPWII+TR V+   DDP+HV+ +  +LSVTPLKRAGEPHEVSSMV 
Sbjct: 181 ACEWAKDNIRTNAVAPWIIRTRFVKPPTDDPIHVEVMNHMLSVTPLKRAGEPHEVSSMVA 240

Query: 241 FLCLPAASYITGQLFVIDGGHTVKAYPIPD 270
           FLCLPAASYITGQ+F IDGGHTVKA+P+PD
Sbjct: 241 FLCLPAASYITGQVFCIDGGHTVKAFPVPD 268

[Close]

BLAST of CSPI03G32040 vs. NCBI nr
Match: XP_004137777.1 (tropinone reductase homolog [Cucumis sativus] >KGN58842.1 hypothetical protein Csa_002580 [Cucumis sativus])

HSP 1 Score: 533.9 bits (1374), Expect = 8.2e-148
Identity = 270/270 (100.00%), Postives = 270/270 (100.00%), Query Frame = 0


Query: 1   MANIRNQRWSLKGMTALVTGGSRGIGRATVEELAEFGARVHTCCRSQEDLDKCLKEWEAM 60
           MANIRNQRWSLKGMTALVTGGSRGIGRATVEELAEFGARVHTCCRSQEDLDKCLKEWEAM
Sbjct: 1   MANIRNQRWSLKGMTALVTGGSRGIGRATVEELAEFGARVHTCCRSQEDLDKCLKEWEAM 60

Query: 61  GFKVSGSVCDVQSKEQRKKLMETVSSLFNGTLNILVNNAGRTLSSLKSTVEVTEEDISSV 120
           GFKVSGSVCDVQSKEQRKKLMETVSSLFNGTLNILVNNAGRTLSSLKSTVEVTEEDISSV
Sbjct: 61  GFKVSGSVCDVQSKEQRKKLMETVSSLFNGTLNILVNNAGRTLSSLKSTVEVTEEDISSV 120

Query: 121 MSTNFESSFHFSQLAYPLLKASGNGSIVFISSVSGLTALPFSTPYAASKAAINQITKNLA 180
           MSTNFESSFHFSQLAYPLLKASGNGSIVFISSVSGLTALPFSTPYAASKAAINQITKNLA
Sbjct: 121 MSTNFESSFHFSQLAYPLLKASGNGSIVFISSVSGLTALPFSTPYAASKAAINQITKNLA 180

Query: 181 CEWAKDNIRTNAVAPWIIKTRLVERSNDDPMHVKGIEQLLSVTPLKRAGEPHEVSSMVVF 240
           CEWAKDNIRTNAVAPWIIKTRLVERSNDDPMHVKGIEQLLSVTPLKRAGEPHEVSSMVVF
Sbjct: 181 CEWAKDNIRTNAVAPWIIKTRLVERSNDDPMHVKGIEQLLSVTPLKRAGEPHEVSSMVVF 240

Query: 241 LCLPAASYITGQLFVIDGGHTVKAYPIPDL 271
           LCLPAASYITGQLFVIDGGHTVKAYPIPDL
Sbjct: 241 LCLPAASYITGQLFVIDGGHTVKAYPIPDL 270

[Close]

BLAST of CSPI03G32040 vs. NCBI nr
Match: XP_008442511.1 (PREDICTED: tropinone reductase homolog [Cucumis melo] >KAA0044114.1 tropinone reductase-like protein [Cucumis melo var. makuwa] >TYK25024.1 tropinone reductase-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 509.2 bits (1310), Expect = 2.2e-140
Identity = 256/270 (94.81%), Postives = 262/270 (97.04%), Query Frame = 0


Query: 1   MANIRNQRWSLKGMTALVTGGSRGIGRATVEELAEFGARVHTCCRSQEDLDKCLKEWEAM 60
           MANIRNQRWSLKG TALVTGGSRGIGRATVEELAEFGARVHTCCRSQEDLDKCLKEWE M
Sbjct: 1   MANIRNQRWSLKGKTALVTGGSRGIGRATVEELAEFGARVHTCCRSQEDLDKCLKEWEEM 60

Query: 61  GFKVSGSVCDVQSKEQRKKLMETVSSLFNGTLNILVNNAGRTLSSLKSTVEVTEEDISSV 120
           GF+VSGSVCDVQSKEQRKKLMETVSSLFNGTLNILVNNAGRTLSSLKSTVEVTEE+ISSV
Sbjct: 61  GFEVSGSVCDVQSKEQRKKLMETVSSLFNGTLNILVNNAGRTLSSLKSTVEVTEEEISSV 120

Query: 121 MSTNFESSFHFSQLAYPLLKASGNGSIVFISSVSGLTALPFSTPYAASKAAINQITKNLA 180
           MSTNFE+S HFSQLAYPLLKASGNGSIVFISSVSGL ALPFSTPYAASKAAINQ+TKNLA
Sbjct: 121 MSTNFEASLHFSQLAYPLLKASGNGSIVFISSVSGLMALPFSTPYAASKAAINQMTKNLA 180

Query: 181 CEWAKDNIRTNAVAPWIIKTRLVERSNDDPMHVKGIEQLLSVTPLKRAGEPHEVSSMVVF 240
           CEWAKDNIRTNAVAPWIIKTR VE SNDDPMHVKG+EQ+LSVTPLKRAGEPHEVSSMV F
Sbjct: 181 CEWAKDNIRTNAVAPWIIKTRFVESSNDDPMHVKGMEQILSVTPLKRAGEPHEVSSMVAF 240

Query: 241 LCLPAASYITGQLFVIDGGHTVKAYPIPDL 271
           LCLPAASYITGQLFVIDGGHTVKAYPI DL
Sbjct: 241 LCLPAASYITGQLFVIDGGHTVKAYPISDL 270

[Close]

BLAST of CSPI03G32040 vs. NCBI nr
Match: XP_038904663.1 (tropinone reductase homolog [Benincasa hispida])

HSP 1 Score: 468.4 bits (1204), Expect = 4.2e-128
Identity = 237/270 (87.78%), Postives = 251/270 (92.96%), Query Frame = 0


Query: 1   MANIRNQRWSLKGMTALVTGGSRGIGRATVEELAEFGARVHTCCRSQEDLDKCLKEWEAM 60
           MAN RNQRWSLKGMTALVTGGSRGIGRATVEELAEFGA VHTCCRSQE+LDKCLKEWE M
Sbjct: 1   MANNRNQRWSLKGMTALVTGGSRGIGRATVEELAEFGATVHTCCRSQENLDKCLKEWEEM 60

Query: 61  GFKVSGSVCDVQSKEQRKKLMETVSSLFNGTLNILVNNAGRTLSSLKSTVEVTEEDISSV 120
           G KVSGSVCDVQS+EQRKKLMETVSSLFNG+LNILVNNAG T S LK+  EVTEE+I SV
Sbjct: 61  GLKVSGSVCDVQSREQRKKLMETVSSLFNGSLNILVNNAG-TTSELKTAAEVTEEEIWSV 120

Query: 121 MSTNFESSFHFSQLAYPLLKASGNGSIVFISSVSGLTALPFSTPYAASKAAINQITKNLA 180
           M+TNFE+SFH SQLAYPLLKASGNG+IVFISSVSGL ALP+STPYAASKAAINQ+TKNLA
Sbjct: 121 MNTNFEASFHSSQLAYPLLKASGNGNIVFISSVSGLMALPYSTPYAASKAAINQMTKNLA 180

Query: 181 CEWAKDNIRTNAVAPWIIKTRLVERSNDDPMHVKGIEQLLSVTPLKRAGEPHEVSSMVVF 240
           CEWAKDNIR NAVAPWII+TR VE  NDDP++VK +EQLLSVTPLKRAGEPHEVSSMV F
Sbjct: 181 CEWAKDNIRVNAVAPWIIRTRFVEPPNDDPIYVKSMEQLLSVTPLKRAGEPHEVSSMVAF 240

Query: 241 LCLPAASYITGQLFVIDGGHTVKAYPIPDL 271
           LCLPAASYITGQLFVIDGGHTVKAYPI DL
Sbjct: 241 LCLPAASYITGQLFVIDGGHTVKAYPISDL 269

[Close]

BLAST of CSPI03G32040 vs. NCBI nr
Match: XP_022971600.1 (tropinone reductase homolog [Cucurbita maxima])

HSP 1 Score: 432.2 bits (1110), Expect = 3.4e-117
Identity = 217/270 (80.37%), Postives = 244/270 (90.37%), Query Frame = 0


Query: 1   MANI-RNQRWSLKGMTALVTGGSRGIGRATVEELAEFGARVHTCCRSQEDLDKCLKEWEA 60
           MAN  R+QRWSL+GMTALVTGG+ GIGRATVEELA FGA VHTC RSQ DLDKCLKEWE 
Sbjct: 1   MANTSRDQRWSLQGMTALVTGGTHGIGRATVEELAAFGAIVHTCSRSQGDLDKCLKEWEE 60

Query: 61  MGFKVSGSVCDVQSKEQRKKLMETVSSLFNGTLNILVNNAGRTLSSLKSTVEVTEEDISS 120
           MG  VSGSVCDV S+EQRK+LMETVSSLFNG+LNILVNNAG   S+ KS++EVTEE++SS
Sbjct: 61  MGLNVSGSVCDVHSREQRKRLMETVSSLFNGSLNILVNNAG--TSAYKSSLEVTEEELSS 120

Query: 121 VMSTNFESSFHFSQLAYPLLKASGNGSIVFISSVSGLTALPFSTPYAASKAAINQITKNL 180
           VMSTNFE+SFHFSQLA+PL+KASGNGSIVF+SSV+GL +LP+STPYAASKAAINQIT+NL
Sbjct: 121 VMSTNFEASFHFSQLAHPLMKASGNGSIVFMSSVAGLMSLPYSTPYAASKAAINQITRNL 180

Query: 181 ACEWAKDNIRTNAVAPWIIKTRLVERSNDDPMHVKGIEQLLSVTPLKRAGEPHEVSSMVV 240
           ACEWAKDNIRTNAVAPWII+TR V+   DDP+HV+ +  +LSVTPLKRAGEPHEVSSMV 
Sbjct: 181 ACEWAKDNIRTNAVAPWIIRTRFVKPPTDDPIHVEVMNHMLSVTPLKRAGEPHEVSSMVA 240

Query: 241 FLCLPAASYITGQLFVIDGGHTVKAYPIPD 270
           FLCLPAASYITGQ+F IDGGHTV A+PIPD
Sbjct: 241 FLCLPAASYITGQVFCIDGGHTVTAFPIPD 268

[Close]

BLAST of CSPI03G32040 vs. NCBI nr
Match: XP_022941371.1 (tropinone reductase homolog [Cucurbita moschata])

HSP 1 Score: 431.8 bits (1109), Expect = 4.4e-117
Identity = 215/270 (79.63%), Postives = 245/270 (90.74%), Query Frame = 0


Query: 1   MANI-RNQRWSLKGMTALVTGGSRGIGRATVEELAEFGARVHTCCRSQEDLDKCLKEWEA 60
           MAN  R+QRWSL+GMTALVTGG+ GIGRATVEELA FGA VHTC RSQ DLDKCLKEWE 
Sbjct: 1   MANTSRDQRWSLQGMTALVTGGTHGIGRATVEELAAFGATVHTCSRSQGDLDKCLKEWEE 60

Query: 61  MGFKVSGSVCDVQSKEQRKKLMETVSSLFNGTLNILVNNAGRTLSSLKSTVEVTEEDISS 120
           MGF VSGSVCDV S+EQR +L+ETVSSLFNG+LNILVNNAG   S+ KS++EVTEE++SS
Sbjct: 61  MGFNVSGSVCDVHSREQRMQLIETVSSLFNGSLNILVNNAG--TSAYKSSLEVTEEELSS 120

Query: 121 VMSTNFESSFHFSQLAYPLLKASGNGSIVFISSVSGLTALPFSTPYAASKAAINQITKNL 180
           VMSTNFE++FHFSQLA+PL+KASGNGSIVF+SSV+GL +LP+STPYAASKAAINQIT+NL
Sbjct: 121 VMSTNFEAAFHFSQLAHPLMKASGNGSIVFMSSVAGLMSLPYSTPYAASKAAINQITRNL 180

Query: 181 ACEWAKDNIRTNAVAPWIIKTRLVERSNDDPMHVKGIEQLLSVTPLKRAGEPHEVSSMVV 240
           ACEWAKDNIRTNAVAPWII+TR V+   DDP+HV+ +  +LSVTPLKRAGEPHEVSSMV 
Sbjct: 181 ACEWAKDNIRTNAVAPWIIRTRFVKPPTDDPIHVEVMNHMLSVTPLKRAGEPHEVSSMVA 240

Query: 241 FLCLPAASYITGQLFVIDGGHTVKAYPIPD 270
           FLCLPAASYITGQ+F IDGGHTVKA+P+PD
Sbjct: 241 FLCLPAASYITGQVFCIDGGHTVKAFPVPD 268

[Close]

BLAST of CSPI03G32040 vs. TAIR 10
Match: AT5G06060.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 311.6 bits (797), Expect = 6.2e-85
Identity = 157/260 (60.38%), Postives = 198/260 (76.15%), Query Frame = 0


Query: 6   NQRWSLKGMTALVTGGSRGIGRATVEELAEFGARVHTCCRSQEDLDKCLKEWEAMGFKVS 65
           ++RWSL G TALVTGG+RGIGRA VEELA+FGA+VHTC R+QE+L+ CL +W+A G  VS
Sbjct: 4   DKRWSLAGKTALVTGGTRGIGRAVVEELAKFGAKVHTCSRNQEELNACLNDWKANGLVVS 63

Query: 66  GSVCDVQSKEQRKKLMETVSSLFNGTLNILVNNAGRTLSSLKSTVEVTEEDISSVMSTNF 125
           GSVCD   ++QR+KL++  SS F+G LNIL+NN G  +   K TVE + E+ + +MSTN 
Sbjct: 64  GSVCDASVRDQREKLIQEASSAFSGKLNILINNVGTNVR--KPTVEYSSEEYAKIMSTNL 123

Query: 126 ESSFHFSQLAYPLLKASGNGSIVFISSVSGLTALPFSTPYAASKAAINQITKNLACEWAK 185
           ES+FH SQ+A+PLLKASG GSIVFISSV+GL  L   + Y A+K A+NQ+T+NLACEWA 
Sbjct: 124 ESAFHLSQIAHPLLKASGVGSIVFISSVAGLVHLSSGSIYGATKGALNQLTRNLACEWAS 183

Query: 186 DNIRTNAVAPWIIKTRLVERSNDDPMHVKGIEQLLSVTPLKRAGEPHEVSSMVVFLCLPA 245
           DNIRTN VAPW IKT LVE   +     + +E ++S TPL R GEP EVSS+V FLCLPA
Sbjct: 184 DNIRTNCVAPWYIKTSLVETLLEKK---EFVEAVVSRTPLGRVGEPEEVSSLVAFLCLPA 243

Query: 246 ASYITGQLFVIDGGHTVKAY 266
           +SYITGQ+  +DGG TV  +
Sbjct: 244 SSYITGQVISVDGGFTVNGF 258

[Close]

BLAST of CSPI03G32040 vs. TAIR 10
Match: AT2G29260.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 301.2 bits (770), Expect = 8.3e-82
Identity = 152/259 (58.69%), Postives = 193/259 (74.52%), Query Frame = 0


Query: 7   QRWSLKGMTALVTGGSRGIGRATVEELAEFGARVHTCCRSQEDLDKCLKEWEAMGFKVSG 66
           +RWSL GM+ALVTGG+RGIGRA VEELA  GA VHTC R++ +L+ CL +W   GF+V+G
Sbjct: 64  ERWSLNGMSALVTGGTRGIGRAIVEELAGLGAEVHTCARNEYELENCLSDWNRSGFRVAG 123

Query: 67  SVCDVQSKEQRKKLMETVSSLFNGTLNILVNNAGRTLSSLKSTVEVTEEDISSVMSTNFE 126
           SVCDV  + QR+ LMETVSS+F G L+ILVNN G  +   K  VE T  + S++MSTNFE
Sbjct: 124 SVCDVSDRSQREALMETVSSVFEGKLHILVNNVGTNIR--KPMVEFTAGEFSTLMSTNFE 183

Query: 127 SSFHFSQLAYPLLKASGNGSIVFISSVSGLTALPFSTPYAASKAAINQITKNLACEWAKD 186
           S FH  QLAYPLL+ S  GS+VFISSVSG  +L   +  +++K AINQ+T++LACEWAKD
Sbjct: 184 SVFHLCQLAYPLLRESKAGSVVFISSVSGFVSLKNMSVQSSTKGAINQLTRSLACEWAKD 243

Query: 187 NIRTNAVAPWIIKTRLVERSNDDPMHVKGIEQLLSVTPLKRAGEPHEVSSMVVFLCLPAA 246
           NIR NAVAPW IKT +VE+   +  +   +E++ SVTPL R GEP EVSS V FLCLPA+
Sbjct: 244 NIRINAVAPWYIKTSMVEQVLSNKEY---LEEVYSVTPLGRLGEPREVSSAVAFLCLPAS 303

Query: 247 SYITGQLFVIDGGHTVKAY 266
           SYITGQ+  +DGG ++  +
Sbjct: 304 SYITGQILCVDGGMSINGF 317

[Close]

BLAST of CSPI03G32040 vs. TAIR 10
Match: AT2G29370.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 297.7 bits (761), Expect = 9.2e-81
Identity = 152/262 (58.02%), Postives = 197/262 (75.19%), Query Frame = 0


Query: 8   RWSLKGMTALVTGGSRGIGRATVEELAEFGARVHTCCRSQEDLDKCLKEWEAMGFKVSGS 67
           +WSL+GMTALVTGGS+G+G+A VEELA  GARVHTC R +  L + L+EW+A G +V+ S
Sbjct: 13  KWSLEGMTALVTGGSKGLGKAVVEELAMLGARVHTCARDETQLQESLREWQAKGLQVTTS 72

Query: 68  VCDVQSKEQRKKLMETVSSLFNGTLNILVNNAGRTLSSLKSTVEVTEEDISSVMSTNFES 127
           VCDV S++QR+KLMETVSSLF G L+ILV N G  +  LK T E T E+ S +++TN ES
Sbjct: 73  VCDVSSRDQREKLMETVSSLFQGKLSILVPNVG--IGVLKPTTECTAEEFSFIIATNLES 132

Query: 128 SFHFSQLAYPLLKASGNGSIVFISSVSGLTALPFSTPYAASKAAINQITKNLACEWAKDN 187
           +FHFSQLA+PLLKASG+G+IV +SSV+G+  L  ++ Y A+K A+NQ+ +NLACEWA DN
Sbjct: 133 TFHFSQLAHPLLKASGSGNIVLMSSVAGVVNLGNTSIYGATKGAMNQLARNLACEWASDN 192

Query: 188 IRTNAVAPWIIKTRLVERSNDDPMHVKGI-EQLLSVTPLKRAGEPHEVSSMVVFLCLPAA 247
           IR N+V PW I T     S  D +  K + E++ SVTPL+R GE +EVSS+V FLCLPAA
Sbjct: 193 IRANSVCPWFITT----PSTKDFLGDKDVKEKVESVTPLRRVGEANEVSSLVAFLCLPAA 252

Query: 248 SYITGQLFVIDGGHTVKAYPIP 269
           SYITGQ   +DGG T+  + +P
Sbjct: 253 SYITGQTICVDGGFTINGFSLP 268

[Close]

BLAST of CSPI03G32040 vs. TAIR 10
Match: AT2G29360.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 296.6 bits (758), Expect = 2.0e-80
Identity = 154/264 (58.33%), Postives = 194/264 (73.48%), Query Frame = 0


Query: 8   RWSLKGMTALVTGGSRGIGRATVEELAEFGARVHTCCRSQEDLDKCLKEWEAMGFKVSGS 67
           RWSL GMTALVTGGS+GIG A VEELA  GAR+HTC R +  L + L++W+A GF+V+ S
Sbjct: 13  RWSLVGMTALVTGGSKGIGEAVVEELATLGARIHTCARDETQLQESLRKWQAKGFQVTTS 72

Query: 68  VCDVQSKEQRKKLMETVSSLFNGTLNILVNNAGRTLSSLKSTVEVTEEDISSVMSTNFES 127
           VCDV S+++R+KLMETVS++F G LNILVNN G  +  +K T++ T ED S  M+TN ES
Sbjct: 73  VCDVSSRDKREKLMETVSTIFEGKLNILVNNVGTCI--VKPTLQHTAEDFSFTMATNLES 132

Query: 128 SFHFSQLAYPLLKASGNGSIVFISSVSGLTALPFSTPYAASKAAINQITKNLACEWAKDN 187
           +FH SQLA+PLLKASG+GSIV ISSVSG+  +  ++ Y  SK A+NQ+ +NLACEWA DN
Sbjct: 133 AFHLSQLAHPLLKASGSGSIVLISSVSGVVHVNGASIYGVSKGAMNQLGRNLACEWASDN 192

Query: 188 IRTNAVAPWIIKTRLVERSNDDPMHVKGIEQLLSVTPLKRAGEPHEVSSMVVFLCLPAAS 247
           IRTN+V PW I+T LV  S  +    K +E   S  P+ R GE +EVSS+V FLCLPAAS
Sbjct: 193 IRTNSVCPWFIETPLVTESLSNEEFRKEVE---SRPPMGRVGEVNEVSSLVAFLCLPAAS 252

Query: 248 YITGQLFVIDGGHTVKAY---PIP 269
           YITGQ   +DGG TV  +   P+P
Sbjct: 253 YITGQTICVDGGFTVNGFSFKPLP 271

[Close]

BLAST of CSPI03G32040 vs. TAIR 10
Match: AT1G07450.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 295.8 bits (756), Expect = 3.5e-80
Identity = 154/258 (59.69%), Postives = 193/258 (74.81%), Query Frame = 0


Query: 8   RWSLKGMTALVTGGSRGIGRATVEELAEFGARVHTCCRSQEDLDKCLKEWEAMGFKVSGS 67
           RWSL+GMTALVTGGS+GIG A VEEL  FGARVH C   +  L++CL  W A GF+VSGS
Sbjct: 5   RWSLQGMTALVTGGSKGIGYAIVEELVGFGARVHICDIDETLLNECLSGWHAKGFEVSGS 64

Query: 68  VCDVQSKEQRKKLMETVSSLFNGTLNILVNNAGRTLSSLKSTVEVTEEDISSVMSTNFES 127
           +CDV S+ QR +LM+TVSSLF   LNIL+NN G+ +  LK T+E T ED SS+M+TN ES
Sbjct: 65  ICDVSSRPQRVQLMQTVSSLFGAKLNILINNVGKYI--LKPTLESTAEDFSSLMATNLES 124

Query: 128 SFHFSQLAYPLLKASGNGSIVFISSVSGLTALPFSTPYAASKAAINQITKNLACEWAKDN 187
           +++ SQLA+PLLKASGNG+IVFISSV+G+ +   ST Y  +K A+NQ+ ++LACEWA DN
Sbjct: 125 AYYISQLAHPLLKASGNGNIVFISSVTGVVS-GTSTIYGVTKGALNQLARDLACEWASDN 184

Query: 188 IRTNAVAPWIIKTRLVERSNDDPMHVKGIEQLLSVTPLKRAGEPHEVSSMVVFLCLPAAS 247
           IR N+VAPW+  T LV++  +D +     E + S TPL RA EP EV+S+V FLCLPAAS
Sbjct: 185 IRANSVAPWVTATSLVQKYLEDEIFA---EAMFSRTPLGRACEPREVASLVTFLCLPAAS 244

Query: 248 YITGQLFVIDGGHTVKAY 266
           YITGQ   IDGG TV  +
Sbjct: 245 YITGQTICIDGGFTVNGF 256

The following BLAST results are available for this feature:

BLAST of CSPI03G32040 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P50165	1.8e-89	63.08	Tropinone reductase homolog OS=Datura stramonium OX=4076 PE=2 SV=1	[more]
Q9LHT0	8.7e-84	60.38	Tropinone reductase homolog At5g06060 OS=Arabidopsis thaliana OX=3702 GN=At5g060...	[more]
Q9ZW12	1.2e-80	58.69	Tropinone reductase homolog At2g29260, chloroplastic OS=Arabidopsis thaliana OX=...	[more]
Q9ZW20	1.3e-79	58.02	Tropinone reductase homolog At2g29370 OS=Arabidopsis thaliana OX=3702 GN=At2g293...	[more]
Q9ZW19	2.9e-79	58.33	Tropinone reductase homolog At2g29360 OS=Arabidopsis thaliana OX=3702 GN=SDR PE=...	[more]

BLAST of CSPI03G32040 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LDM3	4.0e-148	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G733960 PE=4 SV=1	[more]
A0A5D3DN54	1.0e-140	94.81	Tropinone reductase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A1S3B5V0	1.0e-140	94.81	tropinone reductase homolog OS=Cucumis melo OX=3656 GN=LOC103486361 PE=4 SV=1	[more]
A0A6J1I667	1.6e-117	80.37	tropinone reductase homolog OS=Cucurbita maxima OX=3661 GN=LOC111470275 PE=4 SV=...	[more]
A0A6J1FKX7	2.1e-117	79.63	tropinone reductase homolog OS=Cucurbita moschata OX=3662 GN=LOC111446681 PE=4 S...	[more]

BLAST of CSPI03G32040 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004137777.1	8.2e-148	100.00	tropinone reductase homolog [Cucumis sativus] >KGN58842.1 hypothetical protein C...	[more]
XP_008442511.1	2.2e-140	94.81	PREDICTED: tropinone reductase homolog [Cucumis melo] >KAA0044114.1 tropinone re...	[more]
XP_038904663.1	4.2e-128	87.78	tropinone reductase homolog [Benincasa hispida]	[more]
XP_022971600.1	3.4e-117	80.37	tropinone reductase homolog [Cucurbita maxima]	[more]
XP_022941371.1	4.4e-117	79.63	tropinone reductase homolog [Cucurbita moschata]	[more]

BLAST of CSPI03G32040 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G06060.1	6.2e-85	60.38	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT2G29260.1	8.3e-82	58.69	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT2G29370.1	9.2e-81	58.02	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT2G29360.1	2.0e-80	58.33	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT1G07450.1	3.5e-80	59.69	NAD(P)-binding Rossmann-fold superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00080	SDRFAMILY	coord: 90..101 score: 53.19 coord: 165..184 score: 40.37 coord: 145..153 score: 53.59
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00081	GDHRDH	coord: 139..155 score: 35.35 coord: 224..244 score: 33.84 coord: 90..101 score: 57.11 coord: 186..203 score: 37.23 coord: 15..32 score: 50.99 coord: 165..184 score: 42.77
None	No IPR available	GENE3D	3.40.50.720		coord: 1..265 e-value: 2.0E-84 score: 284.8
None	No IPR available	PFAM	PF13561	adh_short_C2	coord: 22..261 e-value: 3.2E-58 score: 197.1
None	No IPR available	PIRSR	PIRSR000094-3	PIRSR000094-3	coord: 10..264 e-value: 1.0E-15 score: 55.5
None	No IPR available	PIRSR	PIRSR000095-1	PIRSR000095-1	coord: 17..207 e-value: 3.2E-21 score: 73.6
IPR045000	Tropinone reductase	PANTHER	PTHR42898	TROPINONE REDUCTASE	coord: 5..263
IPR020904	Short-chain dehydrogenase/reductase, conserved site	PROSITE	PS00061	ADH_SHORT	coord: 152..180
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 12..262

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G32040.1	CSPI03G32040.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016491	oxidoreductase activity