CSPI03G31850 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI03G31850
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Descriptionrop guanine nucleotide exchange factor 7-like
LocationChr3: 28646564 .. 28650106 (+)
RNA-Seq ExpressionCSPI03G31850
SyntenyCSPI03G31850
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GCTGTATATAATATATGCTTCATCTAATTACATTTCTTAAGTAAATTAAAACTCCAAAGTAAAGCAACCGTAATTAATTACTTAAATGTTAACATATTACAATCAAATCCCATTTGGTTTTGAATTTTTTGTTTTCAAATCTCTCCTACTGGGGACCAGAAAGCAACATTAACATTAATGGAGAAATAGGCTCTTCATGAGTTGAAGCAAGTATCAATAATCATTTCTTCCTCTTTTACACGCATATAAACTATAAACTTACCGCGCTGATGCTGCTTCTTCTAACCTCCACAAAAAATTCTGGGTTGGTTAAAAACTCTGATGGACTTAACCTTCACCCACCAACAACATACTGAACCGATTCCACTTACAAATCTTTCCCCCACCCCCTCCGTTTGCTTCAACCCTTTTTCCAATTTGGGCAACTGGGTTTCCAAGTCCGCTCGAAAACTCTGCCACAGGCCTCGATTCTCATCTGGGTTTTGCTGCAGAGGGCTGGAATACAGGGGAATGGTCGTGAATAACTCTGCGTTTTTGGAAGTTGTTGCTATGGAAATTGAAGTAAAAGAAGAGAAGTGCGATGTGTTTGATGAAAAGACTGCCTCAATTTCAAGTTCTGATTTGTTGAAACCTGTGGAAACAGAGGAGGTGGAGGAACGGAGTTTGAAAGATGAAGAGTCGTCGTCGTCGTCGTCTTCTTCCTCTTCCTCTTCCTCTTCCTCTTCCTCTTCCTCTTCTTCAACATCAGCAGCTTCAATGGGATGGCCTCTTCACGAAACGGTGGTTGAAGGTGAAGAAGACAGAGTGGTGGAGAGGGAAGTGTCAACAGACTCAGGTATGGTGATGGTGACGTGTTAATAGCACAAGATGAATGAATGATATTGTGGATTTGTGGTGATGAAATTTGATGATGATTTTTAGAAACAGAGATGATGAAAGAGAGGTTTTCAAAGCTGTTGCTGGGTGAAGATATGTCAGGGAGTGGAAATGGAGTCTGTACTGCTTTGGCTATATCCAATGCCATTACCAATCTCTGTGGTATGTCAACACCTTTAAATCCTATATATATATATATTATCAAAAAGTTTTCTAATCAACTCATGTTTATTCTTAAATTTTTAATTTCAGGGCATTCCATTCTAAAAACCGCCTTGTTGCACTTTTATCGTATTCTCAACTTCTACTAAACTCACACACTACTTTCAAAAAAATGTCAAGAGCAAAAATCTCTTACCATTCACTAGTTTTCACCACTAAATATATAATTGTAATTATTTTGAATTAATTAATAGAAATAAACCCAAAGAAGGGTGTAAAGATTTTAATCTTTTACCTCAAACACATGTCAATTATTATTGCTTAGTTAGATTTTTTTTTCTAGTTAAAGTACATAGAGATGAATGGATGAGTTCAAATTTGAAATCCACCTTTTTATTGTCTATTTTCAAATCTTTGCAAACTTATTTACTTCTGTAACTAATAACTTTGTTGGTTGAGAAATGAGCTTTCAATTTTTAATTATTTATAATTTTCATGAATATGTATTGTCCAACTGGCTAATAGTATACTATTAGTAAATTGTTTTCTTCTTCAGCTCAGCGAATGATTTTGCTAACTTATTTATGACTATGTGTCTGATTCTGATAAAGTAAGTGAGAATTGGTTAAAATGTATACTTCAACTTAAAGGGTTAAGAATATCCTATCATTTTGTTTATCAAAATAATAAAAGTTTTAACCCAAAAATAAAGAAGAAAATTCTGATGGTTGGGTTGTTGAACTTGAAGGCAGTGTATTTGGACAGTTATGGAGATTAGAACCTTTGGAAGCAGAGAGAAAAGCAATGTGGGGAAGAGAGATGGAATTTTTGCTTTGTGTTAGCAATCACATCGTTGAACTGATTCCCATTTGGCAGACATTCCCAGATGGAACCAAGCTTGAGGTTCATTTCACTTCATTTCAGTTCCATTCAATTCTATCTATTACCAAAAAAAACTTAGTAGTCTTTTATTTTCTTCTTTCTTTCCATCACCATCACTCACCAGATCATGACTTGCCGCCCCCGTTCAGATCTTTACGTCAATCTCCCAGCTCTTCGCAAACTAGACCACATGCTCCTCGTAAGTTCATATTTAAACTAAATACCCTCTTTTGTTTCTTCCTCACTAAAGTTTTCATAATTTCCACTGCAGGATATCTTGGACAGTTTTGTTGATTCTGAGTTCTGCTACATTGATCAAGGGATCCTTGCCACGGATCAAACCGATGCCTCTTCCTCTTTCCGTAAACTTCTCGAGCGCCAAGACGACAAATGGTGGCTCCCTATTCCAAGAGTTCCTAATGGAGGCCTCTCTGAAGCTTCAACAAGGCACCTACAACACAAGCGAGACTGCACGAATCAAATTCTTAAAGCTGCCATGGCCATCAACTCTGTTACTTTAGCTGACATGGACGTCCCCATTTCATATTTGGAAGGTCTTCCAAAAGTAAGCCAATTGTATAATTTAAACATACTATATCATTTAATGCAACATCAACATCATCGAAACCCTGTTTCAGAATGGAAGAGCTAGCTTGGGAGAAGCGATTTACAAGTACATTTCTTCAGATGAATTTTCCCCTGCTTTGTTATTGGAATGTCTAGACATATCTTCTGAACACCAAGCCATAGAAATAGCCAACCGAGTTGAGAGCGCAATATATGCATGGCGCACCAAAGGAATAGCCTCCAATAATTCAAAATCGTCTTGGGAAATGCTTAAAGAACTGATGATCGATGCAGACAAGAGCGAAGTATTAGCTGAAAGAGCAGAATTAGTACTTCTTTGCCTCAAGCAACAGTTTCCTAATCTCCCTCAAACAAGCCTAGACATGAGCAAAATCCAATACAACAAGGTGGGTGTGTTTCTTTCTTTGTCTGTTACCATACTGAGAATTTTCCAATAAAATTTGGTTCTTTTTTGTTGTTGTTGTTTAGGATGTCGGGAAAGCTATACTAGAGAGCTATTCAAGAGTGCTGGAGAGCTTGGCTTATAACATTGTGGCACGTATTGAAGATCTGCTTTATGTGAATGAATTGACGAAGCATTCAGATCAAATTCCTGGGATTTCCCAACTGGGAATTGTTGCTCATAATAGCAGTAATCGAATACATATCTCGATGCCATTTTCCACTTCTCCTTGCAACACGAATTTCATAAAACCCAGTTTTTCGTCTGTGGATCTTGTTGGAACCAGATCGCCCATACCCAAACCTCCGCAATGTGGTCTTGAAGAATCAGAAAAATCTGACTAATTACAAAGTTCTCTGTAGAATAACACAATACAAAGATTCAAAGGAAAGCATACTTCGTCTGCTGTAAATTTGGATTTTCAATATTGGTTTAAGAAATATAGTTAATGATTAAAGCATATGCAACTTCTAATTTCAAAAATGTCGTTGTAGTATAGTGGTAAGTATTCCCGCCTGTCACGCGGGTGACCCGGGTTCGATCCCCGGCAACGGCGTTTTATTAAAGTACCTTTCTTTTTTCCTTTACTTCCA

mRNA sequence

GCTGTATATAATATATGCTTCATCTAATTACATTTCTTAAGTAAATTAAAACTCCAAAGTAAAGCAACCGTAATTAATTACTTAAATGTTAACATATTACAATCAAATCCCATTTGGTTTTGAATTTTTTGTTTTCAAATCTCTCCTACTGGGGACCAGAAAGCAACATTAACATTAATGGAGAAATAGGCTCTTCATGAGTTGAAGCAAGTATCAATAATCATTTCTTCCTCTTTTACACGCATATAAACTATAAACTTACCGCGCTGATGCTGCTTCTTCTAACCTCCACAAAAAATTCTGGGTTGGTTAAAAACTCTGATGGACTTAACCTTCACCCACCAACAACATACTGAACCGATTCCACTTACAAATCTTTCCCCCACCCCCTCCGTTTGCTTCAACCCTTTTTCCAATTTGGGCAACTGGGTTTCCAAGTCCGCTCGAAAACTCTGCCACAGGCCTCGATTCTCATCTGGGTTTTGCTGCAGAGGGCTGGAATACAGGGGAATGGTCGTGAATAACTCTGCGTTTTTGGAAGTTGTTGCTATGGAAATTGAAGTAAAAGAAGAGAAGTGCGATGTGTTTGATGAAAAGACTGCCTCAATTTCAAGTTCTGATTTGTTGAAACCTGTGGAAACAGAGGAGGTGGAGGAACGGAGTTTGAAAGATGAAGAGTCGTCGTCGTCGTCGTCTTCTTCCTCTTCCTCTTCCTCTTCCTCTTCCTCTTCCTCTTCTTCAACATCAGCAGCTTCAATGGGATGGCCTCTTCACGAAACGGTGGTTGAAGGTGAAGAAGACAGAGTGGTGGAGAGGGAAGTGTCAACAGACTCAGGTATGGTGATGGTGACGTGTTAATAGCACAAGATGAATGAATGATATTGTGGATTTGTGGTGATGAAATTTGATGATGATTTTTAGAAACAGAGATGATGAAAGAGAGGTTTTCAAAGCTGTTGCTGGGTGAAGATATGTCAGGGAGTGGAAATGGAGTCTGTACTGCTTTGGCTATATCCAATGCCATTACCAATCTCTGTGGCAGTGTATTTGGACAGTTATGGAGATTAGAACCTTTGGAAGCAGAGAGAAAAGCAATGTGGGGAAGAGAGATGGAATTTTTGCTTTGTGTTAGCAATCACATCGTTGAACTGATTCCCATTTGGCAGACATTCCCAGATGGAACCAAGCTTGAGATCATGACTTGCCGCCCCCGTTCAGATCTTTACGTCAATCTCCCAGCTCTTCGCAAACTAGACCACATGCTCCTCGATATCTTGGACAGTTTTGTTGATTCTGAGTTCTGCTACATTGATCAAGGGATCCTTGCCACGGATCAAACCGATGCCTCTTCCTCTTTCCGTAAACTTCTCGAGCGCCAAGACGACAAATGGTGGCTCCCTATTCCAAGAGTTCCTAATGGAGGCCTCTCTGAAGCTTCAACAAGGCACCTACAACACAAGCGAGACTGCACGAATCAAATTCTTAAAGCTGCCATGGCCATCAACTCTGTTACTTTAGCTGACATGGACGTCCCCATTTCATATTTGGAAGGTCTTCCAAAAAATGGAAGAGCTAGCTTGGGAGAAGCGATTTACAAGTACATTTCTTCAGATGAATTTTCCCCTGCTTTGTTATTGGAATGTCTAGACATATCTTCTGAACACCAAGCCATAGAAATAGCCAACCGAGTTGAGAGCGCAATATATGCATGGCGCACCAAAGGAATAGCCTCCAATAATTCAAAATCGTCTTGGGAAATGCTTAAAGAACTGATGATCGATGCAGACAAGAGCGAAGTATTAGCTGAAAGAGCAGAATTAGTACTTCTTTGCCTCAAGCAACAGTTTCCTAATCTCCCTCAAACAAGCCTAGACATGAGCAAAATCCAATACAACAAGGATGTCGGGAAAGCTATACTAGAGAGCTATTCAAGAGTGCTGGAGAGCTTGGCTTATAACATTGTGGCACGTATTGAAGATCTGCTTTATGTGAATGAATTGACGAAGCATTCAGATCAAATTCCTGGGATTTCCCAACTGGGAATTGTTGCTCATAATAGCAGTAATCGAATACATATCTCGATGCCATTTTCCACTTCTCCTTGCAACACGAATTTCATAAAACCCAGTTTTTCGTCTGTGGATCTTGTTGGAACCAGATCGCCCATACCCAAACCTCCGCAATGTGGTCTTGAAGAATCAGAAAAATCTGACTAATTACAAAGTTCTCTGTAGAATAACACAATACAAAGATTCAAAGGAAAGCATACTTCGTCTGCTGTAAATTTGGATTTTCAATATTGGTTTAAGAAATATAGTTAATGATTAAAGCATATGCAACTTCTAATTTCAAAAATGTCGTTGTAGTATAGTGGTAAGTATTCCCGCCTGTCACGCGGGTGACCCGGGTTCGATCCCCGGCAACGGCGTTTTATTAAAGTACCTTTCTTTTTTCCTTTACTTCCA

Coding sequence (CDS)

ATGATGAAAGAGAGGTTTTCAAAGCTGTTGCTGGGTGAAGATATGTCAGGGAGTGGAAATGGAGTCTGTACTGCTTTGGCTATATCCAATGCCATTACCAATCTCTGTGGCAGTGTATTTGGACAGTTATGGAGATTAGAACCTTTGGAAGCAGAGAGAAAAGCAATGTGGGGAAGAGAGATGGAATTTTTGCTTTGTGTTAGCAATCACATCGTTGAACTGATTCCCATTTGGCAGACATTCCCAGATGGAACCAAGCTTGAGATCATGACTTGCCGCCCCCGTTCAGATCTTTACGTCAATCTCCCAGCTCTTCGCAAACTAGACCACATGCTCCTCGATATCTTGGACAGTTTTGTTGATTCTGAGTTCTGCTACATTGATCAAGGGATCCTTGCCACGGATCAAACCGATGCCTCTTCCTCTTTCCGTAAACTTCTCGAGCGCCAAGACGACAAATGGTGGCTCCCTATTCCAAGAGTTCCTAATGGAGGCCTCTCTGAAGCTTCAACAAGGCACCTACAACACAAGCGAGACTGCACGAATCAAATTCTTAAAGCTGCCATGGCCATCAACTCTGTTACTTTAGCTGACATGGACGTCCCCATTTCATATTTGGAAGGTCTTCCAAAAAATGGAAGAGCTAGCTTGGGAGAAGCGATTTACAAGTACATTTCTTCAGATGAATTTTCCCCTGCTTTGTTATTGGAATGTCTAGACATATCTTCTGAACACCAAGCCATAGAAATAGCCAACCGAGTTGAGAGCGCAATATATGCATGGCGCACCAAAGGAATAGCCTCCAATAATTCAAAATCGTCTTGGGAAATGCTTAAAGAACTGATGATCGATGCAGACAAGAGCGAAGTATTAGCTGAAAGAGCAGAATTAGTACTTCTTTGCCTCAAGCAACAGTTTCCTAATCTCCCTCAAACAAGCCTAGACATGAGCAAAATCCAATACAACAAGGATGTCGGGAAAGCTATACTAGAGAGCTATTCAAGAGTGCTGGAGAGCTTGGCTTATAACATTGTGGCACGTATTGAAGATCTGCTTTATGTGAATGAATTGACGAAGCATTCAGATCAAATTCCTGGGATTTCCCAACTGGGAATTGTTGCTCATAATAGCAGTAATCGAATACATATCTCGATGCCATTTTCCACTTCTCCTTGCAACACGAATTTCATAAAACCCAGTTTTTCGTCTGTGGATCTTGTTGGAACCAGATCGCCCATACCCAAACCTCCGCAATGTGGTCTTGAAGAATCAGAAAAATCTGACTAA

Protein sequence

MMKERFSKLLLGEDMSGSGNGVCTALAISNAITNLCGSVFGQLWRLEPLEAERKAMWGREMEFLLCVSNHIVELIPIWQTFPDGTKLEIMTCRPRSDLYVNLPALRKLDHMLLDILDSFVDSEFCYIDQGILATDQTDASSSFRKLLERQDDKWWLPIPRVPNGGLSEASTRHLQHKRDCTNQILKAAMAINSVTLADMDVPISYLEGLPKNGRASLGEAIYKYISSDEFSPALLLECLDISSEHQAIEIANRVESAIYAWRTKGIASNNSKSSWEMLKELMIDADKSEVLAERAELVLLCLKQQFPNLPQTSLDMSKIQYNKDVGKAILESYSRVLESLAYNIVARIEDLLYVNELTKHSDQIPGISQLGIVAHNSSNRIHISMPFSTSPCNTNFIKPSFSSVDLVGTRSPIPKPPQCGLEESEKSD*
Homology
BLAST of CSPI03G31850 vs. ExPASy Swiss-Prot
Match: Q9LZN0 (Rop guanine nucleotide exchange factor 7 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF7 PE=1 SV=1)

HSP 1 Score: 565.5 bits (1456), Expect = 7.4e-160
Identity = 314/488 (64.34%), Postives = 378/488 (77.46%), Query Frame = 0

Query: 109 EVEERSLKDEESSSSSSSSSSSSSSSSSSSSST-SAASMGWPLHETVVEGEEDRVVEREV 168
           EVEE+  +    SS ++       S S +   T S  S  W +    ++GE+ ++  R  
Sbjct: 10  EVEEKGRESSCCSSETTRQEEEEQSPSCTEDFTASPVSSRWSVKN--IDGEKKKI--RSD 69

Query: 169 STDSETEMMKERFSKLLLGEDMSGSGNGVCTALAISNAITNLCGSVFGQLWRLEPLEAER 228
           S  SE EMMKERFSKLLLGEDMSGSGNGVCTALAISNAITNLC ++FGQLWRLEPL  E+
Sbjct: 70  SRVSEVEMMKERFSKLLLGEDMSGSGNGVCTALAISNAITNLCATLFGQLWRLEPLPTEK 129

Query: 229 KAMWGREMEFLLCVSNHIVELIPIWQTFPDGTKLEIMTCRPRSDLYVNLPALRKLDHMLL 288
           K MW REME+LLCVS+HIVE+ P WQTFPDGTKLEIMTCRPRSDLYVNLPALRKLD+MLL
Sbjct: 130 KEMWRREMEWLLCVSDHIVEMTPTWQTFPDGTKLEIMTCRPRSDLYVNLPALRKLDNMLL 189

Query: 289 DILDSFVDSEFCYIDQGILATDQ-TDASSSFRKLLERQDDKWWLPIPRVPNGGLSEASTR 348
           +ILDSF ++EF Y+DQGI+A +   D SSSFRK  +RQ+DKWWLP+PRV  GGL E S +
Sbjct: 190 EILDSFEETEFWYVDQGIMAHESAADGSSSFRKSFQRQEDKWWLPVPRVSPGGLQENSRK 249

Query: 349 HLQHKRDCTNQILKAAMAINSVTLADMDVPISYLEGLPKNGRASLGEAIYKYISSDEFSP 408
            LQHKRDCTNQILKAAMAINS+TLADM++P SYLE LP+ GR+ LG+ IY+YISSD+FSP
Sbjct: 250 QLQHKRDCTNQILKAAMAINSITLADMEIPESYLESLPRKGRSCLGDLIYRYISSDQFSP 309

Query: 409 ALLLECLDISSEHQAIEIANRVESAIYAW--RTKGIASNNSKSSWEMLKELMIDADKSEV 468
             LL+CLD+SSEHQAIEIANRVES+IY W  RT    + N+K+SWEM+KELM+DADK E+
Sbjct: 310 ECLLDCLDLSSEHQAIEIANRVESSIYLWHKRTNSKPATNTKTSWEMVKELMVDADKLEL 369

Query: 469 LAERAELVLLCLKQQFPNLPQTSLDMSKIQYNKDVGKAILESYSRVLESLAYNIVARIED 528
           +A+RAE +LL LKQ+FP LPQT+LDMSKIQYNKD+GK+ILESYSRVLESLA+NIVARI+D
Sbjct: 370 MADRAESLLLSLKQRFPGLPQTALDMSKIQYNKDIGKSILESYSRVLESLAFNIVARIDD 429

Query: 529 LLYVNELTKH-SDQIPGISQLGIVAHNSSNRIHISMPFSTSPCNTNFIKPSFSSVDLVGT 588
           LL+V++LT+H SDQIP  + LG    N+ N      P S +   +N+  PS+S       
Sbjct: 430 LLFVDDLTRHSSDQIP--TTLG----NNGN----DAPKSIAVPVSNYTTPSYSP-SKQEL 482

Query: 589 RSPIPKPP 592
           RS I  PP
Sbjct: 490 RSSITVPP 482

BLAST of CSPI03G31850 vs. ExPASy Swiss-Prot
Match: F4K295 (Rop guanine nucleotide exchange factor 5 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF5 PE=2 SV=1)

HSP 1 Score: 493.0 bits (1268), Expect = 4.7e-138
Identity = 277/524 (52.86%), Postives = 373/524 (71.18%), Query Frame = 0

Query: 84  EKCDVFDEKTASISSSDLLKPVETEEVEERSLKDEESSSSSSSSSSSSSSSSSSSSSTSA 143
           + C   ++K ++++ S  ++ + TE  E  S     +S +S  SSS+++ +SSS     +
Sbjct: 6   KSCAGIEKKRSNLTPS--MEDILTESKERSS-----TSGASYESSSTTTVASSSPPPPPS 65

Query: 144 ASMGWPL----------------HETVVEGEEDRVVEREVSTDSETEMMKERFSKLLLGE 203
             +GWP+                H+ +   ++     +E+++ ++ EMMKERF+KLLLGE
Sbjct: 66  QILGWPIRKASFRKNSKESVNFDHKKLTLHDDSGFKAKEMNS-ADVEMMKERFAKLLLGE 125

Query: 204 DMSGSGNGVCTALAISNAITNLCGSVFGQLWRLEPLEAERKAMWGREMEFLLCVSNHIVE 263
           DMSGSG GVCTALAISNAITNLC ++FGQLWRLEPL +E+K MW REME++L VS+HIVE
Sbjct: 126 DMSGSGKGVCTALAISNAITNLCATIFGQLWRLEPLSSEKKEMWRREMEWILSVSDHIVE 185

Query: 264 LIPIWQTFPDGTKLEIMTCRPRSDLYVNLPALRKLDHMLLDILDSFVDSEFCYIDQGILA 323
           L P  QT+PDG K E+MTCRPR DL++NLPALRKLD+MLLDIL SF  +EF Y+DQGI+A
Sbjct: 186 LTPSTQTYPDGNKFEVMTCRPRFDLFINLPALRKLDNMLLDILASFKKTEFWYVDQGIVA 245

Query: 324 TDQTDASSSFRKLLERQDDKWWLPIPRVPNGGLSEASTRHLQHKRDCTNQILKAAMAINS 383
           + + D S+SFR+ ++RQ++KWWLP+PR+   GL+E +   L HKR+C  QILKAAMAINS
Sbjct: 246 S-ENDGSASFRRKIQRQEEKWWLPVPRLAPNGLTEEARTELNHKRECATQILKAAMAINS 305

Query: 384 VTLADMDVPISYLEGLPKNGRASLGEAIYKYISSDEFSPALLLECLDISSEHQAIEIANR 443
           + L +MDVP SYLE LPKNGR+ LG+ IY+Y++SD+FS   LL+CLD+SSEH A++IANR
Sbjct: 306 LALTEMDVPKSYLETLPKNGRSCLGDVIYRYVTSDKFSAESLLDCLDLSSEHIALDIANR 365

Query: 444 VESAIYAWRTK-----GIASNNS----KSSWEMLKELMIDADKSEVLAERAELVLLCLKQ 503
           VE++IY WR +     G+ +N S    K +WEM+KELM   DK  +L ER+E +L CLKQ
Sbjct: 366 VEASIYVWRRRVQTKLGVNNNTSSTTPKLTWEMVKELMAAGDKRGLLVERSETLLRCLKQ 425

Query: 504 QFPNLPQTSLDMSKIQYNKDVGKAILESYSRVLESLAYNIVARIEDLLYVNELTKHSD-- 563
           +FP+L QTSLD+SKIQ+NKD+GK+ILESYSR LESLA NI+ARI+DLLYV++LTK SD  
Sbjct: 426 RFPSLTQTSLDISKIQWNKDIGKSILESYSRALESLASNIIARIDDLLYVDDLTKQSDDN 485

Query: 564 ---QIPGISQLGIVAHNSSNRIHISMPFSTSPCNTNF-IKPSFS 577
                P +S   I+AH     +   +  S +P  T+F   P FS
Sbjct: 486 NLLSSPAVS--SIIAHKKVVPLPYLISASGTPYRTSFSTTPGFS 518

BLAST of CSPI03G31850 vs. ExPASy Swiss-Prot
Match: Q9M056 (Rop guanine nucleotide exchange factor 6 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF6 PE=2 SV=1)

HSP 1 Score: 427.2 bits (1097), Expect = 3.1e-118
Identity = 254/515 (49.32%), Postives = 346/515 (67.18%), Query Frame = 0

Query: 107 TEEVEERSLKDEESSSSSSSSSSSSSSSSSSSSSTSAASMGWPLHETVVEGEEDRVVE-- 166
           TE   + SL    S   SSS  SS +  S SS  T +  +GWPL +        ++ +  
Sbjct: 30  TESTTDSSLSSSSSGVGSSSGRSSVAERSVSSPPTKSQILGWPLGQGSWRKSSGKMKKKT 89

Query: 167 ---------REVSTD-SETEMMKERFSKLLLGEDMSGSGNGVCTALAISNAITNLCGSVF 226
                    + V T+ SE E++KER +KLLLGEDMSGSG GVC ALAISNAITNL  ++ 
Sbjct: 90  PTKIDDFGFKRVGTETSEIELLKERMAKLLLGEDMSGSGEGVCPALAISNAITNLYAAIL 149

Query: 227 GQLWRLEPLEAERKAMWGREMEFLLCVSNHIVELIPIWQTFPDGTKLEIMTCRPRSDLYV 286
           GQ WRLEP+ +E+K MW RE+E LL VS+HIVEL+P +Q FP+G K+E+M CRPRSDL+ 
Sbjct: 150 GQQWRLEPIPSEKKLMWRREIEVLLSVSDHIVELVPSFQNFPNGNKIEVMNCRPRSDLFT 209

Query: 287 NLPALRKLDHMLLDILDSFVDSEFCYIDQGILATDQTDASSSFRKLLERQDDKWWLPIPR 346
            LPALRKLD+ML++ILDSF ++EF Y+DQGI+A +    S+SFR+      DKWWLP+PR
Sbjct: 210 CLPALRKLDNMLIEILDSFGETEFWYVDQGIVAAESA-RSNSFRE----DGDKWWLPLPR 269

Query: 347 VPNGGLSEASTRHLQHKRDCTNQILKAAMAINSVTLADMDVPISYLEGLPKNGRASLGEA 406
           VP+ GL+E + + L H R+ TNQILKA M+INS+ LA+M+VP SYLE LPKNGR+ LG+ 
Sbjct: 270 VPSDGLTEQTRKKLDHTREFTNQILKACMSINSIALAEMEVPQSYLEALPKNGRSCLGDF 329

Query: 407 IYKYISSDEFSPALLLECLDISSEHQAIEIANRVESAIYAWRTKGIASN------NSKSS 466
           +Y+ I+SD FS   LLE +D+SSE   +E+ANRVE+++Y WR +  + +      ++ + 
Sbjct: 330 LYRNITSDNFSADHLLESIDLSSELAVVEMANRVEASMYVWRRRAHSRHLISLYRSTSTR 389

Query: 467 WEML-KELMI---DADKSEVLAERAELVLLCLKQQFPNLPQTSLDMSKIQYNKDVGKAIL 526
           W M+ KE+M+   D DK E+ AERAE +L+ LKQ+FP L QT+LD SKIQYNKDVGK+IL
Sbjct: 390 WGMIVKEMMMHQTDGDKREIFAERAESLLIRLKQRFPGLRQTALDTSKIQYNKDVGKSIL 449

Query: 527 ESYSRVLESLAYNIVARIEDLLYVNELTKH---------SDQIPGISQLGIVAHNSSNRI 586
           ESYSRVLESLAY+I  RIE++L++++++K          SD++  +S+      + S R 
Sbjct: 450 ESYSRVLESLAYSIGVRIEEVLFMDDISKDDGDGDDDSCSDKLRLLSKEAASGGSGSLRE 509

Query: 587 HISMP--FSTSPCNTNFIKPSFSSVDLVGTRSPIP 589
            +S P  FS S   T+    + S      + SP+P
Sbjct: 510 KLSAPSLFSVSFSGTSTPYRTLSFSASTPSYSPMP 539

BLAST of CSPI03G31850 vs. ExPASy Swiss-Prot
Match: Q93ZY2 (Rop guanine nucleotide exchange factor 1 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF1 PE=1 SV=2)

HSP 1 Score: 425.2 bits (1092), Expect = 1.2e-117
Identity = 263/524 (50.19%), Postives = 346/524 (66.03%), Query Frame = 0

Query: 83  EEKCDVFDEKTASISSSDLLKPVETEEVEERSLKDEESSSSSSSS--SSSSSSSSSSSSS 142
           EE  +V  E+  S S S               + + ESSSS S        +SSS  SS 
Sbjct: 7   EEDDEVSSERCGSYSPS-------------ADISESESSSSFSCHRFDGEGASSSIPSSP 66

Query: 143 TSAASMGW----PLHETVVEGEEDRVVEREVSTD-SETEMMKERFSKLLLGEDMSGSGNG 202
              A  G+    P+   V+ G++    +++   D SE EMMKERF+KLLLGEDMSG G G
Sbjct: 67  RVVAGRGFYFPAPVMLPVIGGKDVVWDDKQPDNDLSEIEMMKERFAKLLLGEDMSGGGKG 126

Query: 203 VCTALAISNAITNLCGSVFGQLWRLEPLEAERKAMWGREMEFLLCVSNHIVELIPIWQTF 262
           VCTALAISNAITNL  +VFG+LWRLEPL  ++KAMW RE+E+LLCVS+ IVELIP  Q F
Sbjct: 127 VCTALAISNAITNLSATVFGELWRLEPLAPQKKAMWRRELEWLLCVSDSIVELIPSIQQF 186

Query: 263 PDGTKLEIMTCRPRSDLYVNLPALRKLDHMLLDILDSFVDSEFCYIDQGILATDQTDASS 322
           P G   EIM  RPRSDLY NLPAL+KLD ML+D+LD+F D+EF Y D+GI+  D  D  S
Sbjct: 187 PGGGTYEIMETRPRSDLYANLPALKKLDAMLIDMLDAFSDTEFWYTDRGIVLGD-CDKDS 246

Query: 323 SFRKLLERQDDKWWLPIPRVPNGGLSEASTRHLQHKRDCTNQILKAAMAINSVTLADMDV 382
                  RQ+DKWWLP P+VP  GLSE + + LQ  RD  NQILKAA+AINS  LA+M++
Sbjct: 247 YNSPASVRQEDKWWLPCPKVPPNGLSEEARKKLQQCRDFANQILKAALAINSGVLAEMEI 306

Query: 383 PISYLEGLPKNGRASLGEAIYKYISSDEFSPALLLECLDISSEHQAIEIANRVESAIYAW 442
           P  YLE LPK+G+  LGE IY+Y+++++FSP  LL+CLD+SSEHQ +EIANR+E+A++ W
Sbjct: 307 PDPYLETLPKSGKECLGEIIYQYLTANKFSPECLLDCLDLSSEHQTLEIANRIEAAVHVW 366

Query: 443 RTKGIASNNSK-----SSW-EMLKELMIDADKSEVLAERAELVLLCLKQQFPNLPQTSLD 502
           R K    +  +     SSW   +K L+ D ++++ L +RAE +L  L+ +FP LPQT+LD
Sbjct: 367 RQKNGRRHKKQAKLKLSSWGGKVKGLVNDNERNDFLVQRAETLLQSLRIRFPGLPQTTLD 426

Query: 503 MSKIQYNKDVGKAILESYSRVLESLAYNIVARIEDLLYVNELTKHSDQIPGISQLGIVAH 562
           M+KIQYNKDVG++ILESYSRV+ES+A+NI ARI+D+LYV++  + S     IS    ++ 
Sbjct: 427 MNKIQYNKDVGQSILESYSRVMESMAFNITARIDDVLYVDDAMRRS-----ISVTESLSL 486

Query: 563 NSSNRIHISMPFS--TSPCNTNFIKPSFSSVDLVGTRSPIPKPP 592
            S N ++    FS  +SP  + F  P+ S    V +RSP   PP
Sbjct: 487 FSINGLNPQKAFSVQSSPHGSPFATPALS----VASRSPRRAPP 507

BLAST of CSPI03G31850 vs. ExPASy Swiss-Prot
Match: A4IJ27 (Rop guanine nucleotide exchange factor 3 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF3 PE=2 SV=1)

HSP 1 Score: 408.3 bits (1048), Expect = 1.5e-112
Identity = 227/429 (52.91%), Postives = 316/429 (73.66%), Query Frame = 0

Query: 123 SSSSSSSSSSSSSSSSSSTSAASMGWPL-----HETVVEGEE----DRVVEREVSTDSET 182
           S ++S  S     ++S  + A+   WP+         + G E    + +V +E+S + E 
Sbjct: 47  SETTSGFSDQIDETNSFCSEASPCNWPVLTESKSSKCLSGLEMQSNECLVVQEIS-EPEL 106

Query: 183 EMMKERFSKLLLGEDMSGSGNGVCTALAISNAITNLCGSVFGQLWRLEPLEAERKAMWGR 242
           E MKERF+KLLLGEDMSGSG GVCTA+ ISNAITNL  +VFGQ  RLEPLE E++A+W R
Sbjct: 107 ETMKERFAKLLLGEDMSGSGKGVCTAVTISNAITNLYATVFGQNLRLEPLETEKRALWKR 166

Query: 243 EMEFLLCVSNHIVELIPIWQTFPDGTKLEIMTCRPRSDLYVNLPALRKLDHMLLDILDSF 302
           EM  LL V ++IVE IP  Q   +G  +E+M  RPR+D+Y+NLPALRKLD ML++ LDSF
Sbjct: 167 EMNCLLSVCDYIVEFIPRCQNLSNGATVEVMESRPRADIYINLPALRKLDSMLMEALDSF 226

Query: 303 VDSEFCYIDQGILATDQTDASS-SFRK-LLERQDDKWWLPIPRVPNGGLSEASTRHLQHK 362
            ++EF Y ++G L+     +S+ SFRK +++R+++KWWLP+P VP+ GLS+ + + L++K
Sbjct: 227 QNTEFWYAEEGSLSMKSARSSTGSFRKVIVQRKEEKWWLPVPLVPSEGLSDKARKQLKNK 286

Query: 363 RDCTNQILKAAMAINSVTLADMDVPISYLEGLPKNGRASLGEAIYKYIS-SDEFSPALLL 422
           R+ TNQI KAAMAINS  L++M++P SY+  LPK G++S+G++IY+Y+S S  F P  LL
Sbjct: 287 RESTNQIHKAAMAINSSILSEMEIPDSYMTTLPKCGKSSVGDSIYRYMSGSGRFFPEQLL 346

Query: 423 ECLDISSEHQAIEIANRVESAIYAWRTKGIASNNSKSSWEMLKELMID---ADKSEVLAE 482
           +CL+ISSEH+A+++A+RVE+++Y WR K   S NSK+SW M+K+LM      DK+ V+AE
Sbjct: 347 DCLNISSEHEAVQLADRVEASMYTWRRKSCLS-NSKNSWNMVKDLMSTTERTDKNYVMAE 406

Query: 483 RAELVLLCLKQQFPNLPQTSLDMSKIQYNKDVGKAILESYSRVLESLAYNIVARIEDLLY 537
           RAE +L CLKQ++P L QTSLD+ KIQYNKDVGKA+LESYSRVLE LA+NIVA I+D+LY
Sbjct: 407 RAETLLFCLKQRYPELSQTSLDICKIQYNKDVGKAVLESYSRVLEGLAFNIVAWIDDVLY 466

BLAST of CSPI03G31850 vs. ExPASy TrEMBL
Match: A0A0A0LAD8 (PRONE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G733280 PE=4 SV=1)

HSP 1 Score: 1150.2 bits (2974), Expect = 0.0e+00
Identity = 600/622 (96.46%), Postives = 601/622 (96.62%), Query Frame = 0

Query: 1   MDLTFTHQQHTEPIPLTNLSPTPSVCFNPFSNLGNWVSKSARKLCHRPRFSSGFCCRGLE 60
           MDLTFTHQQHTEPIPLTNLSPTPSVCFNPFSNLGNWVSKSARKLCHRPRFSSGFCCRGLE
Sbjct: 1   MDLTFTHQQHTEPIPLTNLSPTPSVCFNPFSNLGNWVSKSARKLCHRPRFSSGFCCRGLE 60

Query: 61  YRGMVVNNSAFLEVVAMEIEVKEEKCDVFDEKTASISSSDLLKPVETEEVEERSLKDEE- 120
           YRGMVVNNSAFLEVVAMEIEVKEEKCDVFDEKTASISSSDLLKPVETEEVEERSLKDEE 
Sbjct: 61  YRGMVVNNSAFLEVVAMEIEVKEEKCDVFDEKTASISSSDLLKPVETEEVEERSLKDEES 120

Query: 121 -------------------SSSSSSSSSSSSSSSSSSSSSTSAASMGWPLHETVVEGEED 180
                              SSSSSSSSSSSSSSSSSSSSSTSAASMGWPLHETVVEGEED
Sbjct: 121 SSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSTSAASMGWPLHETVVEGEED 180

Query: 181 RVVEREVSTDSETEMMKERFSKLLLGEDMSGSGNGVCTALAISNAITNLCGSVFGQLWRL 240
           RVVEREVSTDSETEMMKERFSKLLLGEDMSGSGNGVCTALAISNAITNLCGSVFGQLWRL
Sbjct: 181 RVVEREVSTDSETEMMKERFSKLLLGEDMSGSGNGVCTALAISNAITNLCGSVFGQLWRL 240

Query: 241 EPLEAERKAMWGREMEFLLCVSNHIVELIPIWQTFPDGTKLEIMTCRPRSDLYVNLPALR 300
           EPLEAERKAMWGREMEFLLCVSNHIVELIPIWQTFPDGTKLEIMTCRPRSDLYVNLPALR
Sbjct: 241 EPLEAERKAMWGREMEFLLCVSNHIVELIPIWQTFPDGTKLEIMTCRPRSDLYVNLPALR 300

Query: 301 KLDHMLLDILDSFVDSEFCYIDQGILATDQTDASSSFRKLLERQDDKWWLPIPRVPNGGL 360
           KLDHMLLDILDSFVDSEFCYIDQGILATDQTDASSSFRKLLERQDDKWWLPIPRVPNGGL
Sbjct: 301 KLDHMLLDILDSFVDSEFCYIDQGILATDQTDASSSFRKLLERQDDKWWLPIPRVPNGGL 360

Query: 361 SEASTRHLQHKRDCTNQILKAAMAINSVTLADMDVPISYLEGLPKNGRASLGEAIYKYIS 420
           SEASTRHLQHKRDCTNQILKAAMAINSVTLADMDVPISYLEGLPKNGRASLGEAIYKYIS
Sbjct: 361 SEASTRHLQHKRDCTNQILKAAMAINSVTLADMDVPISYLEGLPKNGRASLGEAIYKYIS 420

Query: 421 SDEFSPALLLECLDISSEHQAIEIANRVESAIYAWRTKGIASNNSKSSWEMLKELMIDAD 480
           SDEFSPALLLECLDISSEHQAIEIANRVESA+YAWRTKGIASNNSKSSWEMLKELMIDAD
Sbjct: 421 SDEFSPALLLECLDISSEHQAIEIANRVESAMYAWRTKGIASNNSKSSWEMLKELMIDAD 480

Query: 481 KSEVLAERAELVLLCLKQQFPNLPQTSLDMSKIQYNKDVGKAILESYSRVLESLAYNIVA 540
           KSEVLAERAELVLLCLKQQFPNLPQTSLDMSKIQYNKDVGKAILESYSRVLESLAYNIVA
Sbjct: 481 KSEVLAERAELVLLCLKQQFPNLPQTSLDMSKIQYNKDVGKAILESYSRVLESLAYNIVA 540

Query: 541 RIEDLLYVNELTKHSDQIPGISQLGIVAHNSSNRIHISMPFSTSPCNTNFIKPSFSSVDL 600
           RIEDLLYVNELTKHSDQIPGISQLGIVAHNSSNRIHISMPFSTSP NTNFIKPSFSSVDL
Sbjct: 541 RIEDLLYVNELTKHSDQIPGISQLGIVAHNSSNRIHISMPFSTSPYNTNFIKPSFSSVDL 600

Query: 601 VGTRSPIPKPPQCGLEESEKSD 603
           VGTRSPIPKPPQCGLEESEKSD
Sbjct: 601 VGTRSPIPKPPQCGLEESEKSD 622

BLAST of CSPI03G31850 vs. ExPASy TrEMBL
Match: A0A5A7TMM7 (Rop guanine nucleotide exchange factor 7-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold236G004810 PE=4 SV=1)

HSP 1 Score: 1071.6 bits (2770), Expect = 1.1e-309
Identity = 562/609 (92.28%), Postives = 572/609 (93.92%), Query Frame = 0

Query: 1   MDLTFTHQQHTEPIPLTNLSPTPSVCFNPFSNLGNWVSKSARKLCHRPRFSSGFCCRGLE 60
           MDLTFTHQQH EPIPLTN SPTPS CFNPFSNLGNWVSKSARKLCHR R S+GFCCRGLE
Sbjct: 1   MDLTFTHQQHIEPIPLTNHSPTPSDCFNPFSNLGNWVSKSARKLCHRARSSTGFCCRGLE 60

Query: 61  YRGMVVNNSAFLEVVAMEIEVKEEKCDVF-------DEKTASISSSDLLKPVETEEVEER 120
           YRGMVVNNSAFLEVVAMEIE KEEKCDVF       DEKTAS SSSDLLK VETEEVEE+
Sbjct: 61  YRGMVVNNSAFLEVVAMEIEAKEEKCDVFENARKVSDEKTASNSSSDLLKSVETEEVEEQ 120

Query: 121 SLKDEESSSSSSSSSSSSSSSSSSSSSTSAASMGWPLHETVVEGEEDRVVEREVSTDSET 180
           S KDEE       SSSSSSSSSSSSSSTSAASMGWPLH+ V EGEED  VE+EVSTDSE 
Sbjct: 121 SSKDEE-------SSSSSSSSSSSSSSTSAASMGWPLHKRVGEGEEDG-VEKEVSTDSEM 180

Query: 181 EMMKERFSKLLLGEDMSGSGNGVCTALAISNAITNLCGSVFGQLWRLEPLEAERKAMWGR 240
           EMMKERFSKLLLGEDMSGSGNGVCTALAISNAITNLCGSVFGQLWRLEPLEAERKAMWGR
Sbjct: 181 EMMKERFSKLLLGEDMSGSGNGVCTALAISNAITNLCGSVFGQLWRLEPLEAERKAMWGR 240

Query: 241 EMEFLLCVSNHIVELIPIWQTFPDGTKLEIMTCRPRSDLYVNLPALRKLDHMLLDILDSF 300
           EMEFLLCVSNHIVELIPIWQTFPDGTKLEIMTCRPRSDLYVNLPALRKLDHMLLD+LDSF
Sbjct: 241 EMEFLLCVSNHIVELIPIWQTFPDGTKLEIMTCRPRSDLYVNLPALRKLDHMLLDMLDSF 300

Query: 301 VDSEFCYIDQGILATDQTDASSSFRKLLERQDDKWWLPIPRVPNGGLSEASTRHLQHKRD 360
           VDSEF YIDQGILATDQ DASSSFRKLLERQDDKWWLPIPRVPNGGLSEASTRHL HKRD
Sbjct: 301 VDSEFWYIDQGILATDQADASSSFRKLLERQDDKWWLPIPRVPNGGLSEASTRHLHHKRD 360

Query: 361 CTNQILKAAMAINSVTLADMDVPISYLEGLPKNGRASLGEAIYKYISSDEFSPALLLECL 420
           CTNQILKAAMAINSVTLADMDVPISYLE LPKNGRASLGEAIYKYISSDEFSPA LLECL
Sbjct: 361 CTNQILKAAMAINSVTLADMDVPISYLEALPKNGRASLGEAIYKYISSDEFSPAWLLECL 420

Query: 421 DISSEHQAIEIANRVESAIYAWRTKGIASNNSKSSWEMLKELMIDADKSEVLAERAELVL 480
           DISSEHQAIEIANRVESA+YAWR+KGIASNNSKSSWEMLKELMIDADKSEVLAERAELVL
Sbjct: 421 DISSEHQAIEIANRVESAMYAWRSKGIASNNSKSSWEMLKELMIDADKSEVLAERAELVL 480

Query: 481 LCLKQQFPNLPQTSLDMSKIQYNKDVGKAILESYSRVLESLAYNIVARIEDLLYVNELTK 540
           LCLKQQFPNLPQTSLDMSKIQYNKDVGKAILESYSRVLESLAYNIVARIEDLLYVNELTK
Sbjct: 481 LCLKQQFPNLPQTSLDMSKIQYNKDVGKAILESYSRVLESLAYNIVARIEDLLYVNELTK 540

Query: 541 HSDQIPGISQLGIVAHNSSNRIHISMPFSTSPCNTNFIKPSFSSVDLVGTRSPIPKPPQC 600
           HSDQIPGISQLGIVAHNSSNRI IS+PFSTSP NT FIKPSFS+VDLVGTRSP PKPPQC
Sbjct: 541 HSDQIPGISQLGIVAHNSSNRIQISVPFSTSPYNTKFIKPSFSAVDLVGTRSPTPKPPQC 600

Query: 601 GLEESEKSD 603
           GLEESEKS+
Sbjct: 601 GLEESEKSE 601

BLAST of CSPI03G31850 vs. ExPASy TrEMBL
Match: A0A1S3B5B2 (rop guanine nucleotide exchange factor 7-like OS=Cucumis melo OX=3656 GN=LOC103486343 PE=4 SV=1)

HSP 1 Score: 1071.6 bits (2770), Expect = 1.1e-309
Identity = 562/609 (92.28%), Postives = 572/609 (93.92%), Query Frame = 0

Query: 1   MDLTFTHQQHTEPIPLTNLSPTPSVCFNPFSNLGNWVSKSARKLCHRPRFSSGFCCRGLE 60
           MDLTFTHQQH EPIPLTN SPTPS CFNPFSNLGNWVSKSARKLCHR R S+GFCCRGLE
Sbjct: 1   MDLTFTHQQHIEPIPLTNHSPTPSDCFNPFSNLGNWVSKSARKLCHRARSSTGFCCRGLE 60

Query: 61  YRGMVVNNSAFLEVVAMEIEVKEEKCDVF-------DEKTASISSSDLLKPVETEEVEER 120
           YRGMVVNNSAFLEVVAMEIE KEEKCDVF       DEKTAS SSSDLLK VETEEVEE+
Sbjct: 61  YRGMVVNNSAFLEVVAMEIEAKEEKCDVFENARKVSDEKTASNSSSDLLKSVETEEVEEQ 120

Query: 121 SLKDEESSSSSSSSSSSSSSSSSSSSSTSAASMGWPLHETVVEGEEDRVVEREVSTDSET 180
           S KDEE       SSSSSSSSSSSSSSTSAASMGWPLH+ V EGEED  VE+EVSTDSE 
Sbjct: 121 SSKDEE-------SSSSSSSSSSSSSSTSAASMGWPLHKRVGEGEEDG-VEKEVSTDSEM 180

Query: 181 EMMKERFSKLLLGEDMSGSGNGVCTALAISNAITNLCGSVFGQLWRLEPLEAERKAMWGR 240
           EMMKERFSKLLLGEDMSGSGNGVCTALAISNAITNLCGSVFGQLWRLEPLEAERKAMWGR
Sbjct: 181 EMMKERFSKLLLGEDMSGSGNGVCTALAISNAITNLCGSVFGQLWRLEPLEAERKAMWGR 240

Query: 241 EMEFLLCVSNHIVELIPIWQTFPDGTKLEIMTCRPRSDLYVNLPALRKLDHMLLDILDSF 300
           EMEFLLCVSNHIVELIPIWQTFPDGTKLEIMTCRPRSDLYVNLPALRKLDHMLLD+LDSF
Sbjct: 241 EMEFLLCVSNHIVELIPIWQTFPDGTKLEIMTCRPRSDLYVNLPALRKLDHMLLDMLDSF 300

Query: 301 VDSEFCYIDQGILATDQTDASSSFRKLLERQDDKWWLPIPRVPNGGLSEASTRHLQHKRD 360
           VDSEF YIDQGILATDQ DASSSFRKLLERQDDKWWLPIPRVPNGGLSEASTRHL HKRD
Sbjct: 301 VDSEFWYIDQGILATDQADASSSFRKLLERQDDKWWLPIPRVPNGGLSEASTRHLHHKRD 360

Query: 361 CTNQILKAAMAINSVTLADMDVPISYLEGLPKNGRASLGEAIYKYISSDEFSPALLLECL 420
           CTNQILKAAMAINSVTLADMDVPISYLE LPKNGRASLGEAIYKYISSDEFSPA LLECL
Sbjct: 361 CTNQILKAAMAINSVTLADMDVPISYLEALPKNGRASLGEAIYKYISSDEFSPAWLLECL 420

Query: 421 DISSEHQAIEIANRVESAIYAWRTKGIASNNSKSSWEMLKELMIDADKSEVLAERAELVL 480
           DISSEHQAIEIANRVESA+YAWR+KGIASNNSKSSWEMLKELMIDADKSEVLAERAELVL
Sbjct: 421 DISSEHQAIEIANRVESAMYAWRSKGIASNNSKSSWEMLKELMIDADKSEVLAERAELVL 480

Query: 481 LCLKQQFPNLPQTSLDMSKIQYNKDVGKAILESYSRVLESLAYNIVARIEDLLYVNELTK 540
           LCLKQQFPNLPQTSLDMSKIQYNKDVGKAILESYSRVLESLAYNIVARIEDLLYVNELTK
Sbjct: 481 LCLKQQFPNLPQTSLDMSKIQYNKDVGKAILESYSRVLESLAYNIVARIEDLLYVNELTK 540

Query: 541 HSDQIPGISQLGIVAHNSSNRIHISMPFSTSPCNTNFIKPSFSSVDLVGTRSPIPKPPQC 600
           HSDQIPGISQLGIVAHNSSNRI IS+PFSTSP NT FIKPSFS+VDLVGTRSP PKPPQC
Sbjct: 541 HSDQIPGISQLGIVAHNSSNRIQISVPFSTSPYNTKFIKPSFSAVDLVGTRSPTPKPPQC 600

Query: 601 GLEESEKSD 603
           GLEESEKS+
Sbjct: 601 GLEESEKSE 601

BLAST of CSPI03G31850 vs. ExPASy TrEMBL
Match: A0A5D3DP03 (Rop guanine nucleotide exchange factor 7-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G001280 PE=4 SV=1)

HSP 1 Score: 1011.5 bits (2614), Expect = 1.4e-291
Identity = 540/609 (88.67%), Postives = 550/609 (90.31%), Query Frame = 0

Query: 1   MDLTFTHQQHTEPIPLTNLSPTPSVCFNPFSNLGNWVSKSARKLCHRPRFSSGFCCRGLE 60
           MDLTFTHQQH EPIPLTN SPTPS CFNPFSNLGNWVSKSARKLCHR R S+GFCCRGLE
Sbjct: 1   MDLTFTHQQHIEPIPLTNHSPTPSDCFNPFSNLGNWVSKSARKLCHRARSSTGFCCRGLE 60

Query: 61  YRGMVVNNSAFLEVVAMEIEVKEEKCDVF-------DEKTASISSSDLLKPVETEEVEER 120
           YRGMVVNNSAFLEVVAMEIE KEEKCDVF       DEKTAS SSSDLLK VETEEVEE+
Sbjct: 61  YRGMVVNNSAFLEVVAMEIEAKEEKCDVFENARKVSDEKTASNSSSDLLKSVETEEVEEQ 120

Query: 121 SLKDEESSSSSSSSSSSSSSSSSSSSSTSAASMGWPLHETVVEGEEDRVVEREVSTDSET 180
           S KDEE       SSSSSSSSSSSSSSTSAASMGWPLH+ V EGEED  VE+EVSTDSE 
Sbjct: 121 SSKDEE-------SSSSSSSSSSSSSSTSAASMGWPLHKRVGEGEEDG-VEKEVSTDSEM 180

Query: 181 EMMKERFSKLLLGEDMSGSGNGVCTALAISNAITNLCGSVFGQLWRLEPLEAERKAMWGR 240
           EMMKERFSKLLLGEDMSGSGNGVCTALAISNAITNLCGSVFGQLWRLEPLEAERKAMWGR
Sbjct: 181 EMMKERFSKLLLGEDMSGSGNGVCTALAISNAITNLCGSVFGQLWRLEPLEAERKAMWGR 240

Query: 241 EMEFLLCVSNHIVELIPIWQTFPDGTKLEIMTCRPRSDLYVNLPALRKLDHMLLDILDSF 300
           EMEFLLC                      IMTCRPRSDLYVNLPALRKLDHMLLD+LDSF
Sbjct: 241 EMEFLLC----------------------IMTCRPRSDLYVNLPALRKLDHMLLDMLDSF 300

Query: 301 VDSEFCYIDQGILATDQTDASSSFRKLLERQDDKWWLPIPRVPNGGLSEASTRHLQHKRD 360
           VDSEF YIDQGILATDQ DASSSFRKLLERQDDKWWLPIPRVPNGGLSEASTRHL HKRD
Sbjct: 301 VDSEFWYIDQGILATDQADASSSFRKLLERQDDKWWLPIPRVPNGGLSEASTRHLHHKRD 360

Query: 361 CTNQILKAAMAINSVTLADMDVPISYLEGLPKNGRASLGEAIYKYISSDEFSPALLLECL 420
           CTNQILKAAMAINSVTLADMDVPISYLE LPKNGRASLGEAIYKYISSDEFSPA LLECL
Sbjct: 361 CTNQILKAAMAINSVTLADMDVPISYLEALPKNGRASLGEAIYKYISSDEFSPAWLLECL 420

Query: 421 DISSEHQAIEIANRVESAIYAWRTKGIASNNSKSSWEMLKELMIDADKSEVLAERAELVL 480
           DISSEHQAIEIANRVESA+YAWR+KGIASNNSKSSWEMLKELMIDADKSEVLAERAELVL
Sbjct: 421 DISSEHQAIEIANRVESAMYAWRSKGIASNNSKSSWEMLKELMIDADKSEVLAERAELVL 480

Query: 481 LCLKQQFPNLPQTSLDMSKIQYNKDVGKAILESYSRVLESLAYNIVARIEDLLYVNELTK 540
           LCLKQQFPNLPQTSLDMSKIQYNKDVGKAILESYSRVLESLAYNIVARIEDLLYVNELTK
Sbjct: 481 LCLKQQFPNLPQTSLDMSKIQYNKDVGKAILESYSRVLESLAYNIVARIEDLLYVNELTK 540

Query: 541 HSDQIPGISQLGIVAHNSSNRIHISMPFSTSPCNTNFIKPSFSSVDLVGTRSPIPKPPQC 600
           HSDQIPGISQLGIVAHNSSNRI IS+PFSTSP NT FIKPSFS+VDLVGTRSP PKPPQC
Sbjct: 541 HSDQIPGISQLGIVAHNSSNRIQISVPFSTSPYNTKFIKPSFSAVDLVGTRSPTPKPPQC 579

Query: 601 GLEESEKSD 603
           GLEESEKS+
Sbjct: 601 GLEESEKSE 579

BLAST of CSPI03G31850 vs. ExPASy TrEMBL
Match: A0A6J1CWY5 (rop guanine nucleotide exchange factor 7-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111015048 PE=4 SV=1)

HSP 1 Score: 746.9 bits (1927), Expect = 6.6e-212
Identity = 426/615 (69.27%), Postives = 479/615 (77.89%), Query Frame = 0

Query: 1   MDLTFTHQ-QHTEPIPLTNLS---PTPSV----CFNPFSNLGNWVSKSARKLCHRPRFSS 60
           MD+ F HQ Q   PI   N S     P++    C NPFS L  WV KSARK+     F S
Sbjct: 1   MDVNFAHQEQERVPIRFRNHSLKLCLPNLVNHDCSNPFSILRRWVWKSARKV-----FPS 60

Query: 61  GFCCRGLEYRGMVVNNS------AFLEVVAMEIEV-----KEEKCDVFDEKTASISSSDL 120
           GFCCRGLEY+GMVVNNS      AFLE VAMEI V     KEEKCD+F            
Sbjct: 61  GFCCRGLEYKGMVVNNSTFCSSPAFLEEVAMEIRVEDKEGKEEKCDMFGN---------- 120

Query: 121 LKPVETEEVEERSLKDEESSSSSSSSSSSSSSSSSSSSSTSAASMGWPLHETVVE---GE 180
                 +  +E+    E +SSSSS   +S ++ S    S+S+  MGWPL++TV E   G+
Sbjct: 121 ----AHKVFDEKRGDGESASSSSSDLLTSEATGSHDEESSSSTLMGWPLNQTVGEEGGGD 180

Query: 181 ----EDRVVEREVSTDSETEMMKERFSKLLLGEDMSGSGNGVCTALAISNAITNLCGSVF 240
               +DR +ERE S+ SE E+M ERFSKLLLGEDMSG GNGV TALAISNAITNLC ++F
Sbjct: 181 KLHFDDRELEREASSVSEMEIM-ERFSKLLLGEDMSGCGNGVSTALAISNAITNLCATLF 240

Query: 241 GQLWRLEPLEAERKAMWGREMEFLLCVSNHIVELIPIWQTFPDGTKLEIMTCRPRSDLYV 300
           GQLWRLEPL AERK MW REME+LLCVSNHIVELIP+WQTFPDG+KLEIMTCRPRSDLY 
Sbjct: 241 GQLWRLEPLAAERKGMWRREMEWLLCVSNHIVELIPVWQTFPDGSKLEIMTCRPRSDLYA 300

Query: 301 NLPALRKLDHMLLDILDSFVDSEFCYIDQGILATDQTDASSSFRKLLERQDDKWWLPIPR 360
           NLPALRKLDHMLLDILDSFVDSEF Y+DQGILA +     SSFRKLLERQ+DKWWLP+PR
Sbjct: 301 NLPALRKLDHMLLDILDSFVDSEFWYVDQGILAAEAD--GSSFRKLLERQEDKWWLPVPR 360

Query: 361 VPNGGLSEASTRHLQHKRDCTNQILKAAMAINSVTLADMDVPISYLEGLPKNGRASLGEA 420
           VP+GGLSEAS R LQHKRDCTNQILKAAMAINSVTLADMDVP SYLE LPKNGRASLGE 
Sbjct: 361 VPSGGLSEASRRQLQHKRDCTNQILKAAMAINSVTLADMDVPDSYLEALPKNGRASLGEG 420

Query: 421 IYKYISSDEFSPALLLECLDISSEHQAIEIANRVESAIYAWRTKGIAS---NNS-----K 480
           IY++ISSDEFSP  LLECLD+SSEHQAIEIANRVE+A+YAWR KG+ S   NNS     K
Sbjct: 421 IYRHISSDEFSPVCLLECLDMSSEHQAIEIANRVEAAMYAWR-KGMGSKPTNNSLRSSAK 480

Query: 481 SSWEMLKELMIDADKSEVLAERAELVLLCLKQQFPNLPQTSLDMSKIQYNKDVGKAILES 540
           SSWEMLKEL+IDADKSE+ AERAE VL CLKQQFPNLPQT+LDMSKIQ+NKDVGKAILES
Sbjct: 481 SSWEMLKELVIDADKSELFAERAESVLYCLKQQFPNLPQTNLDMSKIQHNKDVGKAILES 540

Query: 541 YSRVLESLAYNIVARIEDLLYVNELTKHSDQIPGISQLGIVAHNSSNRIHISMPFSTSPC 582
           YSRVLESLA+NIVARI+DLLYV++L KHSD+IP ISQLGIVAHN+SNRI  S+PFS+SP 
Sbjct: 541 YSRVLESLAFNIVARIDDLLYVDDLAKHSDEIPRISQLGIVAHNTSNRIQFSVPFSSSPY 592

BLAST of CSPI03G31850 vs. NCBI nr
Match: XP_004137923.3 (rop guanine nucleotide exchange factor 7 isoform X1 [Cucumis sativus])

HSP 1 Score: 1156.4 bits (2990), Expect = 0.0e+00
Identity = 600/606 (99.01%), Postives = 601/606 (99.17%), Query Frame = 0

Query: 1   MDLTFTHQQHTEPIPLTNLSPTPSVCFNPFSNLGNWVSKSARKLCHRPRFSSGFCCRGLE 60
           MDLTFTHQQHTEPIPLTNLSPTPSVCFNPFSNLGNWVSKSARKLCHRPRFSSGFCCRGLE
Sbjct: 1   MDLTFTHQQHTEPIPLTNLSPTPSVCFNPFSNLGNWVSKSARKLCHRPRFSSGFCCRGLE 60

Query: 61  YRGMVVNNSAFLEVVAMEIEVKEEKCDVFDEKTASISSSDLLKPVETEEVEERSLKDEE- 120
           YRGMVVNNSAFLEVVAMEIEVKEEKCDVFDEKTASISSSDLLKPVETEEVEERSLKDEE 
Sbjct: 61  YRGMVVNNSAFLEVVAMEIEVKEEKCDVFDEKTASISSSDLLKPVETEEVEERSLKDEES 120

Query: 121 ---SSSSSSSSSSSSSSSSSSSSSTSAASMGWPLHETVVEGEEDRVVEREVSTDSETEMM 180
              SSSSSSSSSSSSSSSSSSSSSTSAASMGWPLHETVVEGEEDRVVEREVSTDSETEMM
Sbjct: 121 SSSSSSSSSSSSSSSSSSSSSSSSTSAASMGWPLHETVVEGEEDRVVEREVSTDSETEMM 180

Query: 181 KERFSKLLLGEDMSGSGNGVCTALAISNAITNLCGSVFGQLWRLEPLEAERKAMWGREME 240
           KERFSKLLLGEDMSGSGNGVCTALAISNAITNLCGSVFGQLWRLEPLEAERKAMWGREME
Sbjct: 181 KERFSKLLLGEDMSGSGNGVCTALAISNAITNLCGSVFGQLWRLEPLEAERKAMWGREME 240

Query: 241 FLLCVSNHIVELIPIWQTFPDGTKLEIMTCRPRSDLYVNLPALRKLDHMLLDILDSFVDS 300
           FLLCVSNHIVELIPIWQTFPDGTKLEIMTCRPRSDLYVNLPALRKLDHMLLDILDSFVDS
Sbjct: 241 FLLCVSNHIVELIPIWQTFPDGTKLEIMTCRPRSDLYVNLPALRKLDHMLLDILDSFVDS 300

Query: 301 EFCYIDQGILATDQTDASSSFRKLLERQDDKWWLPIPRVPNGGLSEASTRHLQHKRDCTN 360
           EFCYIDQGILATDQTDASSSFRKLLERQDDKWWLPIPRVPNGGLSEASTRHLQHKRDCTN
Sbjct: 301 EFCYIDQGILATDQTDASSSFRKLLERQDDKWWLPIPRVPNGGLSEASTRHLQHKRDCTN 360

Query: 361 QILKAAMAINSVTLADMDVPISYLEGLPKNGRASLGEAIYKYISSDEFSPALLLECLDIS 420
           QILKAAMAINSVTLADMDVPISYLEGLPKNGRASLGEAIYKYISSDEFSPALLLECLDIS
Sbjct: 361 QILKAAMAINSVTLADMDVPISYLEGLPKNGRASLGEAIYKYISSDEFSPALLLECLDIS 420

Query: 421 SEHQAIEIANRVESAIYAWRTKGIASNNSKSSWEMLKELMIDADKSEVLAERAELVLLCL 480
           SEHQAIEIANRVESA+YAWRTKGIASNNSKSSWEMLKELMIDADKSEVLAERAELVLLCL
Sbjct: 421 SEHQAIEIANRVESAMYAWRTKGIASNNSKSSWEMLKELMIDADKSEVLAERAELVLLCL 480

Query: 481 KQQFPNLPQTSLDMSKIQYNKDVGKAILESYSRVLESLAYNIVARIEDLLYVNELTKHSD 540
           KQQFPNLPQTSLDMSKIQYNKDVGKAILESYSRVLESLAYNIVARIEDLLYVNELTKHSD
Sbjct: 481 KQQFPNLPQTSLDMSKIQYNKDVGKAILESYSRVLESLAYNIVARIEDLLYVNELTKHSD 540

Query: 541 QIPGISQLGIVAHNSSNRIHISMPFSTSPCNTNFIKPSFSSVDLVGTRSPIPKPPQCGLE 600
           QIPGISQLGIVAHNSSNRIHISMPFSTSP NTNFIKPSFSSVDLVGTRSPIPKPPQCGLE
Sbjct: 541 QIPGISQLGIVAHNSSNRIHISMPFSTSPYNTNFIKPSFSSVDLVGTRSPIPKPPQCGLE 600

Query: 601 ESEKSD 603
           ESEKSD
Sbjct: 601 ESEKSD 606

BLAST of CSPI03G31850 vs. NCBI nr
Match: XP_011651877.2 (rop guanine nucleotide exchange factor 7 isoform X2 [Cucumis sativus])

HSP 1 Score: 1147.9 bits (2968), Expect = 0.0e+00
Identity = 598/606 (98.68%), Postives = 599/606 (98.84%), Query Frame = 0

Query: 1   MDLTFTHQQHTEPIPLTNLSPTPSVCFNPFSNLGNWVSKSARKLCHRPRFSSGFCCRGLE 60
           MDLTFTHQQHTEPIPLTNLSPTPSVCFNPFSNLGNWVSKSARKLCHRPRFSSGFCCRGLE
Sbjct: 1   MDLTFTHQQHTEPIPLTNLSPTPSVCFNPFSNLGNWVSKSARKLCHRPRFSSGFCCRGLE 60

Query: 61  YRGMVVNNSAFLEVVAMEIEVKEEKCDVFDEKTASISSSDLLKPVETEEVEERSLKDEE- 120
           YRGMVVNNSAFLEVVAMEIEVKEEKCDVFDEKTASISSSDLLKPVETEEVEERSLKDEE 
Sbjct: 61  YRGMVVNNSAFLEVVAMEIEVKEEKCDVFDEKTASISSSDLLKPVETEEVEERSLKDEES 120

Query: 121 ---SSSSSSSSSSSSSSSSSSSSSTSAASMGWPLHETVVEGEEDRVVEREVSTDSETEMM 180
              SSSSSSSSSSSSSSSSSSSSSTSAASMGWPLHETVVEGEEDRVVEREVSTDS  EMM
Sbjct: 121 SSSSSSSSSSSSSSSSSSSSSSSSTSAASMGWPLHETVVEGEEDRVVEREVSTDS--EMM 180

Query: 181 KERFSKLLLGEDMSGSGNGVCTALAISNAITNLCGSVFGQLWRLEPLEAERKAMWGREME 240
           KERFSKLLLGEDMSGSGNGVCTALAISNAITNLCGSVFGQLWRLEPLEAERKAMWGREME
Sbjct: 181 KERFSKLLLGEDMSGSGNGVCTALAISNAITNLCGSVFGQLWRLEPLEAERKAMWGREME 240

Query: 241 FLLCVSNHIVELIPIWQTFPDGTKLEIMTCRPRSDLYVNLPALRKLDHMLLDILDSFVDS 300
           FLLCVSNHIVELIPIWQTFPDGTKLEIMTCRPRSDLYVNLPALRKLDHMLLDILDSFVDS
Sbjct: 241 FLLCVSNHIVELIPIWQTFPDGTKLEIMTCRPRSDLYVNLPALRKLDHMLLDILDSFVDS 300

Query: 301 EFCYIDQGILATDQTDASSSFRKLLERQDDKWWLPIPRVPNGGLSEASTRHLQHKRDCTN 360
           EFCYIDQGILATDQTDASSSFRKLLERQDDKWWLPIPRVPNGGLSEASTRHLQHKRDCTN
Sbjct: 301 EFCYIDQGILATDQTDASSSFRKLLERQDDKWWLPIPRVPNGGLSEASTRHLQHKRDCTN 360

Query: 361 QILKAAMAINSVTLADMDVPISYLEGLPKNGRASLGEAIYKYISSDEFSPALLLECLDIS 420
           QILKAAMAINSVTLADMDVPISYLEGLPKNGRASLGEAIYKYISSDEFSPALLLECLDIS
Sbjct: 361 QILKAAMAINSVTLADMDVPISYLEGLPKNGRASLGEAIYKYISSDEFSPALLLECLDIS 420

Query: 421 SEHQAIEIANRVESAIYAWRTKGIASNNSKSSWEMLKELMIDADKSEVLAERAELVLLCL 480
           SEHQAIEIANRVESA+YAWRTKGIASNNSKSSWEMLKELMIDADKSEVLAERAELVLLCL
Sbjct: 421 SEHQAIEIANRVESAMYAWRTKGIASNNSKSSWEMLKELMIDADKSEVLAERAELVLLCL 480

Query: 481 KQQFPNLPQTSLDMSKIQYNKDVGKAILESYSRVLESLAYNIVARIEDLLYVNELTKHSD 540
           KQQFPNLPQTSLDMSKIQYNKDVGKAILESYSRVLESLAYNIVARIEDLLYVNELTKHSD
Sbjct: 481 KQQFPNLPQTSLDMSKIQYNKDVGKAILESYSRVLESLAYNIVARIEDLLYVNELTKHSD 540

Query: 541 QIPGISQLGIVAHNSSNRIHISMPFSTSPCNTNFIKPSFSSVDLVGTRSPIPKPPQCGLE 600
           QIPGISQLGIVAHNSSNRIHISMPFSTSP NTNFIKPSFSSVDLVGTRSPIPKPPQCGLE
Sbjct: 541 QIPGISQLGIVAHNSSNRIHISMPFSTSPYNTNFIKPSFSSVDLVGTRSPIPKPPQCGLE 600

Query: 601 ESEKSD 603
           ESEKSD
Sbjct: 601 ESEKSD 604

BLAST of CSPI03G31850 vs. NCBI nr
Match: XP_008442490.1 (PREDICTED: rop guanine nucleotide exchange factor 7-like [Cucumis melo] >KAA0044128.1 rop guanine nucleotide exchange factor 7-like [Cucumis melo var. makuwa])

HSP 1 Score: 1071.6 bits (2770), Expect = 2.3e-309
Identity = 562/609 (92.28%), Postives = 572/609 (93.92%), Query Frame = 0

Query: 1   MDLTFTHQQHTEPIPLTNLSPTPSVCFNPFSNLGNWVSKSARKLCHRPRFSSGFCCRGLE 60
           MDLTFTHQQH EPIPLTN SPTPS CFNPFSNLGNWVSKSARKLCHR R S+GFCCRGLE
Sbjct: 1   MDLTFTHQQHIEPIPLTNHSPTPSDCFNPFSNLGNWVSKSARKLCHRARSSTGFCCRGLE 60

Query: 61  YRGMVVNNSAFLEVVAMEIEVKEEKCDVF-------DEKTASISSSDLLKPVETEEVEER 120
           YRGMVVNNSAFLEVVAMEIE KEEKCDVF       DEKTAS SSSDLLK VETEEVEE+
Sbjct: 61  YRGMVVNNSAFLEVVAMEIEAKEEKCDVFENARKVSDEKTASNSSSDLLKSVETEEVEEQ 120

Query: 121 SLKDEESSSSSSSSSSSSSSSSSSSSSTSAASMGWPLHETVVEGEEDRVVEREVSTDSET 180
           S KDEE       SSSSSSSSSSSSSSTSAASMGWPLH+ V EGEED  VE+EVSTDSE 
Sbjct: 121 SSKDEE-------SSSSSSSSSSSSSSTSAASMGWPLHKRVGEGEEDG-VEKEVSTDSEM 180

Query: 181 EMMKERFSKLLLGEDMSGSGNGVCTALAISNAITNLCGSVFGQLWRLEPLEAERKAMWGR 240
           EMMKERFSKLLLGEDMSGSGNGVCTALAISNAITNLCGSVFGQLWRLEPLEAERKAMWGR
Sbjct: 181 EMMKERFSKLLLGEDMSGSGNGVCTALAISNAITNLCGSVFGQLWRLEPLEAERKAMWGR 240

Query: 241 EMEFLLCVSNHIVELIPIWQTFPDGTKLEIMTCRPRSDLYVNLPALRKLDHMLLDILDSF 300
           EMEFLLCVSNHIVELIPIWQTFPDGTKLEIMTCRPRSDLYVNLPALRKLDHMLLD+LDSF
Sbjct: 241 EMEFLLCVSNHIVELIPIWQTFPDGTKLEIMTCRPRSDLYVNLPALRKLDHMLLDMLDSF 300

Query: 301 VDSEFCYIDQGILATDQTDASSSFRKLLERQDDKWWLPIPRVPNGGLSEASTRHLQHKRD 360
           VDSEF YIDQGILATDQ DASSSFRKLLERQDDKWWLPIPRVPNGGLSEASTRHL HKRD
Sbjct: 301 VDSEFWYIDQGILATDQADASSSFRKLLERQDDKWWLPIPRVPNGGLSEASTRHLHHKRD 360

Query: 361 CTNQILKAAMAINSVTLADMDVPISYLEGLPKNGRASLGEAIYKYISSDEFSPALLLECL 420
           CTNQILKAAMAINSVTLADMDVPISYLE LPKNGRASLGEAIYKYISSDEFSPA LLECL
Sbjct: 361 CTNQILKAAMAINSVTLADMDVPISYLEALPKNGRASLGEAIYKYISSDEFSPAWLLECL 420

Query: 421 DISSEHQAIEIANRVESAIYAWRTKGIASNNSKSSWEMLKELMIDADKSEVLAERAELVL 480
           DISSEHQAIEIANRVESA+YAWR+KGIASNNSKSSWEMLKELMIDADKSEVLAERAELVL
Sbjct: 421 DISSEHQAIEIANRVESAMYAWRSKGIASNNSKSSWEMLKELMIDADKSEVLAERAELVL 480

Query: 481 LCLKQQFPNLPQTSLDMSKIQYNKDVGKAILESYSRVLESLAYNIVARIEDLLYVNELTK 540
           LCLKQQFPNLPQTSLDMSKIQYNKDVGKAILESYSRVLESLAYNIVARIEDLLYVNELTK
Sbjct: 481 LCLKQQFPNLPQTSLDMSKIQYNKDVGKAILESYSRVLESLAYNIVARIEDLLYVNELTK 540

Query: 541 HSDQIPGISQLGIVAHNSSNRIHISMPFSTSPCNTNFIKPSFSSVDLVGTRSPIPKPPQC 600
           HSDQIPGISQLGIVAHNSSNRI IS+PFSTSP NT FIKPSFS+VDLVGTRSP PKPPQC
Sbjct: 541 HSDQIPGISQLGIVAHNSSNRIQISVPFSTSPYNTKFIKPSFSAVDLVGTRSPTPKPPQC 600

Query: 601 GLEESEKSD 603
           GLEESEKS+
Sbjct: 601 GLEESEKSE 601

BLAST of CSPI03G31850 vs. NCBI nr
Match: TYK25010.1 (rop guanine nucleotide exchange factor 7-like [Cucumis melo var. makuwa])

HSP 1 Score: 1011.5 bits (2614), Expect = 3.0e-291
Identity = 540/609 (88.67%), Postives = 550/609 (90.31%), Query Frame = 0

Query: 1   MDLTFTHQQHTEPIPLTNLSPTPSVCFNPFSNLGNWVSKSARKLCHRPRFSSGFCCRGLE 60
           MDLTFTHQQH EPIPLTN SPTPS CFNPFSNLGNWVSKSARKLCHR R S+GFCCRGLE
Sbjct: 1   MDLTFTHQQHIEPIPLTNHSPTPSDCFNPFSNLGNWVSKSARKLCHRARSSTGFCCRGLE 60

Query: 61  YRGMVVNNSAFLEVVAMEIEVKEEKCDVF-------DEKTASISSSDLLKPVETEEVEER 120
           YRGMVVNNSAFLEVVAMEIE KEEKCDVF       DEKTAS SSSDLLK VETEEVEE+
Sbjct: 61  YRGMVVNNSAFLEVVAMEIEAKEEKCDVFENARKVSDEKTASNSSSDLLKSVETEEVEEQ 120

Query: 121 SLKDEESSSSSSSSSSSSSSSSSSSSSTSAASMGWPLHETVVEGEEDRVVEREVSTDSET 180
           S KDEE       SSSSSSSSSSSSSSTSAASMGWPLH+ V EGEED  VE+EVSTDSE 
Sbjct: 121 SSKDEE-------SSSSSSSSSSSSSSTSAASMGWPLHKRVGEGEEDG-VEKEVSTDSEM 180

Query: 181 EMMKERFSKLLLGEDMSGSGNGVCTALAISNAITNLCGSVFGQLWRLEPLEAERKAMWGR 240
           EMMKERFSKLLLGEDMSGSGNGVCTALAISNAITNLCGSVFGQLWRLEPLEAERKAMWGR
Sbjct: 181 EMMKERFSKLLLGEDMSGSGNGVCTALAISNAITNLCGSVFGQLWRLEPLEAERKAMWGR 240

Query: 241 EMEFLLCVSNHIVELIPIWQTFPDGTKLEIMTCRPRSDLYVNLPALRKLDHMLLDILDSF 300
           EMEFLLC                      IMTCRPRSDLYVNLPALRKLDHMLLD+LDSF
Sbjct: 241 EMEFLLC----------------------IMTCRPRSDLYVNLPALRKLDHMLLDMLDSF 300

Query: 301 VDSEFCYIDQGILATDQTDASSSFRKLLERQDDKWWLPIPRVPNGGLSEASTRHLQHKRD 360
           VDSEF YIDQGILATDQ DASSSFRKLLERQDDKWWLPIPRVPNGGLSEASTRHL HKRD
Sbjct: 301 VDSEFWYIDQGILATDQADASSSFRKLLERQDDKWWLPIPRVPNGGLSEASTRHLHHKRD 360

Query: 361 CTNQILKAAMAINSVTLADMDVPISYLEGLPKNGRASLGEAIYKYISSDEFSPALLLECL 420
           CTNQILKAAMAINSVTLADMDVPISYLE LPKNGRASLGEAIYKYISSDEFSPA LLECL
Sbjct: 361 CTNQILKAAMAINSVTLADMDVPISYLEALPKNGRASLGEAIYKYISSDEFSPAWLLECL 420

Query: 421 DISSEHQAIEIANRVESAIYAWRTKGIASNNSKSSWEMLKELMIDADKSEVLAERAELVL 480
           DISSEHQAIEIANRVESA+YAWR+KGIASNNSKSSWEMLKELMIDADKSEVLAERAELVL
Sbjct: 421 DISSEHQAIEIANRVESAMYAWRSKGIASNNSKSSWEMLKELMIDADKSEVLAERAELVL 480

Query: 481 LCLKQQFPNLPQTSLDMSKIQYNKDVGKAILESYSRVLESLAYNIVARIEDLLYVNELTK 540
           LCLKQQFPNLPQTSLDMSKIQYNKDVGKAILESYSRVLESLAYNIVARIEDLLYVNELTK
Sbjct: 481 LCLKQQFPNLPQTSLDMSKIQYNKDVGKAILESYSRVLESLAYNIVARIEDLLYVNELTK 540

Query: 541 HSDQIPGISQLGIVAHNSSNRIHISMPFSTSPCNTNFIKPSFSSVDLVGTRSPIPKPPQC 600
           HSDQIPGISQLGIVAHNSSNRI IS+PFSTSP NT FIKPSFS+VDLVGTRSP PKPPQC
Sbjct: 541 HSDQIPGISQLGIVAHNSSNRIQISVPFSTSPYNTKFIKPSFSAVDLVGTRSPTPKPPQC 579

Query: 601 GLEESEKSD 603
           GLEESEKS+
Sbjct: 601 GLEESEKSE 579

BLAST of CSPI03G31850 vs. NCBI nr
Match: XP_038905547.1 (rop guanine nucleotide exchange factor 7-like isoform X2 [Benincasa hispida])

HSP 1 Score: 985.3 bits (2546), Expect = 2.3e-283
Identity = 518/609 (85.06%), Postives = 543/609 (89.16%), Query Frame = 0

Query: 1   MDLTFTHQQHTEPIPLTNLSPTPSVCFNPFSNLGNWVSKSARKLCHRPRFSSGFCCRGLE 60
           MDLTFTHQQ TEPIPLTN SPT S CFNPFSNLG WVSKSARKLC + RFS+GFCCRGLE
Sbjct: 1   MDLTFTHQQDTEPIPLTNHSPTRSDCFNPFSNLGYWVSKSARKLCQKARFSTGFCCRGLE 60

Query: 61  YRGMVVNNSAFLEVVAMEIEVKEEKCD-------VFDEKTASISSSDLLKPVETEEVEER 120
           YRGMVVNNSAFLEVVAMEIE KEEKCD       VFDE T S SSSDLL   ET E+EE+
Sbjct: 61  YRGMVVNNSAFLEVVAMEIEGKEEKCDVFGNSQKVFDENTGSTSSSDLLTSKETGEMEEQ 120

Query: 121 SLKDEESSSSSSSSSSSSSSSSSSSSSTSAASMGWPLHETVVEGEEDRVVEREVSTDSET 180
            ++DEESSSSSSSSS             SAASMGWPLH+T  EGEE   VERE+STDSET
Sbjct: 121 RMQDEESSSSSSSSS-------------SAASMGWPLHKTGGEGEEG--VEREISTDSET 180

Query: 181 EMMKERFSKLLLGEDMSGSGNGVCTALAISNAITNLCGSVFGQLWRLEPLEAERKAMWGR 240
           EMMKERFSKLLLGEDMSG GNGV TALAISNAITNLCGSVFGQLWRLEPLEAERKAMW R
Sbjct: 181 EMMKERFSKLLLGEDMSGCGNGVSTALAISNAITNLCGSVFGQLWRLEPLEAERKAMWER 240

Query: 241 EMEFLLCVSNHIVELIPIWQTFPDGTKLEIMTCRPRSDLYVNLPALRKLDHMLLDILDSF 300
           EME LLCVSNHIVELIPIWQTFPDGTKLEIMTCRPRSDLYVNLPALRKLDHMLL ILDSF
Sbjct: 241 EMELLLCVSNHIVELIPIWQTFPDGTKLEIMTCRPRSDLYVNLPALRKLDHMLLGILDSF 300

Query: 301 VDSEFCYIDQGILATDQTDASSSFRKLLERQDDKWWLPIPRVPNGGLSEASTRHLQHKRD 360
           VDSEF Y+DQGILA DQ DASSSFRK+LERQD+KWWLPIPRVPNGGLSE+STR L HKRD
Sbjct: 301 VDSEFWYVDQGILAADQVDASSSFRKVLERQDEKWWLPIPRVPNGGLSESSTRQLHHKRD 360

Query: 361 CTNQILKAAMAINSVTLADMDVPISYLEGLPKNGRASLGEAIYKYISSDEFSPALLLECL 420
           CTNQILKAAMAINS+TLA+MD+PISYLE LPKNGRASLGEAIYK+ISSDEFSP  LLECL
Sbjct: 361 CTNQILKAAMAINSLTLAEMDIPISYLESLPKNGRASLGEAIYKHISSDEFSPVWLLECL 420

Query: 421 DISSEHQAIEIANRVESAIYAWRTKGIASNNSKSSWEMLKELMIDADKSEVLAERAELVL 480
           DISSEHQAIEIANRVE A+Y WR KGIASNNSKSSWEMLKEL+ID DK EVLAERAELVL
Sbjct: 421 DISSEHQAIEIANRVEGAMYVWR-KGIASNNSKSSWEMLKELVIDGDKIEVLAERAELVL 480

Query: 481 LCLKQQFPNLPQTSLDMSKIQYNKDVGKAILESYSRVLESLAYNIVARIEDLLYVNELTK 540
           LCLKQ+FPNLPQTSLDMSKIQYNKDVGKAILESYSRVLESLAYNIVARIEDLLYVNE+TK
Sbjct: 481 LCLKQRFPNLPQTSLDMSKIQYNKDVGKAILESYSRVLESLAYNIVARIEDLLYVNEMTK 540

Query: 541 HSDQIPGISQLGIVAHNSSNRIHISMPFSTSPCNTNFIKPSFSSVDLVGTRSPIPKPPQC 600
           HSDQIPGISQLGIVAHNSSNRI IS+PFS+SP  T FI PSFS+VDLVGTRS IPK PQC
Sbjct: 541 HSDQIPGISQLGIVAHNSSNRIQISVPFSSSPYTTKFINPSFSTVDLVGTRSLIPKAPQC 593

Query: 601 GLEESEKSD 603
           GLEES+KS+
Sbjct: 601 GLEESKKSE 593

BLAST of CSPI03G31850 vs. TAIR 10
Match: AT5G02010.1 (RHO guanyl-nucleotide exchange factor 7 )

HSP 1 Score: 565.5 bits (1456), Expect = 5.3e-161
Identity = 314/488 (64.34%), Postives = 378/488 (77.46%), Query Frame = 0

Query: 109 EVEERSLKDEESSSSSSSSSSSSSSSSSSSSST-SAASMGWPLHETVVEGEEDRVVEREV 168
           EVEE+  +    SS ++       S S +   T S  S  W +    ++GE+ ++  R  
Sbjct: 10  EVEEKGRESSCCSSETTRQEEEEQSPSCTEDFTASPVSSRWSVKN--IDGEKKKI--RSD 69

Query: 169 STDSETEMMKERFSKLLLGEDMSGSGNGVCTALAISNAITNLCGSVFGQLWRLEPLEAER 228
           S  SE EMMKERFSKLLLGEDMSGSGNGVCTALAISNAITNLC ++FGQLWRLEPL  E+
Sbjct: 70  SRVSEVEMMKERFSKLLLGEDMSGSGNGVCTALAISNAITNLCATLFGQLWRLEPLPTEK 129

Query: 229 KAMWGREMEFLLCVSNHIVELIPIWQTFPDGTKLEIMTCRPRSDLYVNLPALRKLDHMLL 288
           K MW REME+LLCVS+HIVE+ P WQTFPDGTKLEIMTCRPRSDLYVNLPALRKLD+MLL
Sbjct: 130 KEMWRREMEWLLCVSDHIVEMTPTWQTFPDGTKLEIMTCRPRSDLYVNLPALRKLDNMLL 189

Query: 289 DILDSFVDSEFCYIDQGILATDQ-TDASSSFRKLLERQDDKWWLPIPRVPNGGLSEASTR 348
           +ILDSF ++EF Y+DQGI+A +   D SSSFRK  +RQ+DKWWLP+PRV  GGL E S +
Sbjct: 190 EILDSFEETEFWYVDQGIMAHESAADGSSSFRKSFQRQEDKWWLPVPRVSPGGLQENSRK 249

Query: 349 HLQHKRDCTNQILKAAMAINSVTLADMDVPISYLEGLPKNGRASLGEAIYKYISSDEFSP 408
            LQHKRDCTNQILKAAMAINS+TLADM++P SYLE LP+ GR+ LG+ IY+YISSD+FSP
Sbjct: 250 QLQHKRDCTNQILKAAMAINSITLADMEIPESYLESLPRKGRSCLGDLIYRYISSDQFSP 309

Query: 409 ALLLECLDISSEHQAIEIANRVESAIYAW--RTKGIASNNSKSSWEMLKELMIDADKSEV 468
             LL+CLD+SSEHQAIEIANRVES+IY W  RT    + N+K+SWEM+KELM+DADK E+
Sbjct: 310 ECLLDCLDLSSEHQAIEIANRVESSIYLWHKRTNSKPATNTKTSWEMVKELMVDADKLEL 369

Query: 469 LAERAELVLLCLKQQFPNLPQTSLDMSKIQYNKDVGKAILESYSRVLESLAYNIVARIED 528
           +A+RAE +LL LKQ+FP LPQT+LDMSKIQYNKD+GK+ILESYSRVLESLA+NIVARI+D
Sbjct: 370 MADRAESLLLSLKQRFPGLPQTALDMSKIQYNKDIGKSILESYSRVLESLAFNIVARIDD 429

Query: 529 LLYVNELTKH-SDQIPGISQLGIVAHNSSNRIHISMPFSTSPCNTNFIKPSFSSVDLVGT 588
           LL+V++LT+H SDQIP  + LG    N+ N      P S +   +N+  PS+S       
Sbjct: 430 LLFVDDLTRHSSDQIP--TTLG----NNGN----DAPKSIAVPVSNYTTPSYSP-SKQEL 482

Query: 589 RSPIPKPP 592
           RS I  PP
Sbjct: 490 RSSITVPP 482

BLAST of CSPI03G31850 vs. TAIR 10
Match: AT5G05940.1 (ROP guanine nucleotide exchange factor 5 )

HSP 1 Score: 493.0 bits (1268), Expect = 3.3e-139
Identity = 277/524 (52.86%), Postives = 373/524 (71.18%), Query Frame = 0

Query: 84  EKCDVFDEKTASISSSDLLKPVETEEVEERSLKDEESSSSSSSSSSSSSSSSSSSSSTSA 143
           + C   ++K ++++ S  ++ + TE  E  S     +S +S  SSS+++ +SSS     +
Sbjct: 6   KSCAGIEKKRSNLTPS--MEDILTESKERSS-----TSGASYESSSTTTVASSSPPPPPS 65

Query: 144 ASMGWPL----------------HETVVEGEEDRVVEREVSTDSETEMMKERFSKLLLGE 203
             +GWP+                H+ +   ++     +E+++ ++ EMMKERF+KLLLGE
Sbjct: 66  QILGWPIRKASFRKNSKESVNFDHKKLTLHDDSGFKAKEMNS-ADVEMMKERFAKLLLGE 125

Query: 204 DMSGSGNGVCTALAISNAITNLCGSVFGQLWRLEPLEAERKAMWGREMEFLLCVSNHIVE 263
           DMSGSG GVCTALAISNAITNLC ++FGQLWRLEPL +E+K MW REME++L VS+HIVE
Sbjct: 126 DMSGSGKGVCTALAISNAITNLCATIFGQLWRLEPLSSEKKEMWRREMEWILSVSDHIVE 185

Query: 264 LIPIWQTFPDGTKLEIMTCRPRSDLYVNLPALRKLDHMLLDILDSFVDSEFCYIDQGILA 323
           L P  QT+PDG K E+MTCRPR DL++NLPALRKLD+MLLDIL SF  +EF Y+DQGI+A
Sbjct: 186 LTPSTQTYPDGNKFEVMTCRPRFDLFINLPALRKLDNMLLDILASFKKTEFWYVDQGIVA 245

Query: 324 TDQTDASSSFRKLLERQDDKWWLPIPRVPNGGLSEASTRHLQHKRDCTNQILKAAMAINS 383
           + + D S+SFR+ ++RQ++KWWLP+PR+   GL+E +   L HKR+C  QILKAAMAINS
Sbjct: 246 S-ENDGSASFRRKIQRQEEKWWLPVPRLAPNGLTEEARTELNHKRECATQILKAAMAINS 305

Query: 384 VTLADMDVPISYLEGLPKNGRASLGEAIYKYISSDEFSPALLLECLDISSEHQAIEIANR 443
           + L +MDVP SYLE LPKNGR+ LG+ IY+Y++SD+FS   LL+CLD+SSEH A++IANR
Sbjct: 306 LALTEMDVPKSYLETLPKNGRSCLGDVIYRYVTSDKFSAESLLDCLDLSSEHIALDIANR 365

Query: 444 VESAIYAWRTK-----GIASNNS----KSSWEMLKELMIDADKSEVLAERAELVLLCLKQ 503
           VE++IY WR +     G+ +N S    K +WEM+KELM   DK  +L ER+E +L CLKQ
Sbjct: 366 VEASIYVWRRRVQTKLGVNNNTSSTTPKLTWEMVKELMAAGDKRGLLVERSETLLRCLKQ 425

Query: 504 QFPNLPQTSLDMSKIQYNKDVGKAILESYSRVLESLAYNIVARIEDLLYVNELTKHSD-- 563
           +FP+L QTSLD+SKIQ+NKD+GK+ILESYSR LESLA NI+ARI+DLLYV++LTK SD  
Sbjct: 426 RFPSLTQTSLDISKIQWNKDIGKSILESYSRALESLASNIIARIDDLLYVDDLTKQSDDN 485

Query: 564 ---QIPGISQLGIVAHNSSNRIHISMPFSTSPCNTNF-IKPSFS 577
                P +S   I+AH     +   +  S +P  T+F   P FS
Sbjct: 486 NLLSSPAVS--SIIAHKKVVPLPYLISASGTPYRTSFSTTPGFS 518

BLAST of CSPI03G31850 vs. TAIR 10
Match: AT3G55660.1 (ROP (rho of plants) guanine nucleotide exchange factor 6 )

HSP 1 Score: 427.2 bits (1097), Expect = 2.2e-119
Identity = 254/515 (49.32%), Postives = 346/515 (67.18%), Query Frame = 0

Query: 107 TEEVEERSLKDEESSSSSSSSSSSSSSSSSSSSSTSAASMGWPLHETVVEGEEDRVVE-- 166
           TE   + SL    S   SSS  SS +  S SS  T +  +GWPL +        ++ +  
Sbjct: 30  TESTTDSSLSSSSSGVGSSSGRSSVAERSVSSPPTKSQILGWPLGQGSWRKSSGKMKKKT 89

Query: 167 ---------REVSTD-SETEMMKERFSKLLLGEDMSGSGNGVCTALAISNAITNLCGSVF 226
                    + V T+ SE E++KER +KLLLGEDMSGSG GVC ALAISNAITNL  ++ 
Sbjct: 90  PTKIDDFGFKRVGTETSEIELLKERMAKLLLGEDMSGSGEGVCPALAISNAITNLYAAIL 149

Query: 227 GQLWRLEPLEAERKAMWGREMEFLLCVSNHIVELIPIWQTFPDGTKLEIMTCRPRSDLYV 286
           GQ WRLEP+ +E+K MW RE+E LL VS+HIVEL+P +Q FP+G K+E+M CRPRSDL+ 
Sbjct: 150 GQQWRLEPIPSEKKLMWRREIEVLLSVSDHIVELVPSFQNFPNGNKIEVMNCRPRSDLFT 209

Query: 287 NLPALRKLDHMLLDILDSFVDSEFCYIDQGILATDQTDASSSFRKLLERQDDKWWLPIPR 346
            LPALRKLD+ML++ILDSF ++EF Y+DQGI+A +    S+SFR+      DKWWLP+PR
Sbjct: 210 CLPALRKLDNMLIEILDSFGETEFWYVDQGIVAAESA-RSNSFRE----DGDKWWLPLPR 269

Query: 347 VPNGGLSEASTRHLQHKRDCTNQILKAAMAINSVTLADMDVPISYLEGLPKNGRASLGEA 406
           VP+ GL+E + + L H R+ TNQILKA M+INS+ LA+M+VP SYLE LPKNGR+ LG+ 
Sbjct: 270 VPSDGLTEQTRKKLDHTREFTNQILKACMSINSIALAEMEVPQSYLEALPKNGRSCLGDF 329

Query: 407 IYKYISSDEFSPALLLECLDISSEHQAIEIANRVESAIYAWRTKGIASN------NSKSS 466
           +Y+ I+SD FS   LLE +D+SSE   +E+ANRVE+++Y WR +  + +      ++ + 
Sbjct: 330 LYRNITSDNFSADHLLESIDLSSELAVVEMANRVEASMYVWRRRAHSRHLISLYRSTSTR 389

Query: 467 WEML-KELMI---DADKSEVLAERAELVLLCLKQQFPNLPQTSLDMSKIQYNKDVGKAIL 526
           W M+ KE+M+   D DK E+ AERAE +L+ LKQ+FP L QT+LD SKIQYNKDVGK+IL
Sbjct: 390 WGMIVKEMMMHQTDGDKREIFAERAESLLIRLKQRFPGLRQTALDTSKIQYNKDVGKSIL 449

Query: 527 ESYSRVLESLAYNIVARIEDLLYVNELTKH---------SDQIPGISQLGIVAHNSSNRI 586
           ESYSRVLESLAY+I  RIE++L++++++K          SD++  +S+      + S R 
Sbjct: 450 ESYSRVLESLAYSIGVRIEEVLFMDDISKDDGDGDDDSCSDKLRLLSKEAASGGSGSLRE 509

Query: 587 HISMP--FSTSPCNTNFIKPSFSSVDLVGTRSPIP 589
            +S P  FS S   T+    + S      + SP+P
Sbjct: 510 KLSAPSLFSVSFSGTSTPYRTLSFSASTPSYSPMP 539

BLAST of CSPI03G31850 vs. TAIR 10
Match: AT4G38430.1 (rho guanyl-nucleotide exchange factor 1 )

HSP 1 Score: 425.2 bits (1092), Expect = 8.5e-119
Identity = 263/524 (50.19%), Postives = 346/524 (66.03%), Query Frame = 0

Query: 83  EEKCDVFDEKTASISSSDLLKPVETEEVEERSLKDEESSSSSSSS--SSSSSSSSSSSSS 142
           EE  +V  E+  S S S               + + ESSSS S        +SSS  SS 
Sbjct: 7   EEDDEVSSERCGSYSPS-------------ADISESESSSSFSCHRFDGEGASSSIPSSP 66

Query: 143 TSAASMGW----PLHETVVEGEEDRVVEREVSTD-SETEMMKERFSKLLLGEDMSGSGNG 202
              A  G+    P+   V+ G++    +++   D SE EMMKERF+KLLLGEDMSG G G
Sbjct: 67  RVVAGRGFYFPAPVMLPVIGGKDVVWDDKQPDNDLSEIEMMKERFAKLLLGEDMSGGGKG 126

Query: 203 VCTALAISNAITNLCGSVFGQLWRLEPLEAERKAMWGREMEFLLCVSNHIVELIPIWQTF 262
           VCTALAISNAITNL  +VFG+LWRLEPL  ++KAMW RE+E+LLCVS+ IVELIP  Q F
Sbjct: 127 VCTALAISNAITNLSATVFGELWRLEPLAPQKKAMWRRELEWLLCVSDSIVELIPSIQQF 186

Query: 263 PDGTKLEIMTCRPRSDLYVNLPALRKLDHMLLDILDSFVDSEFCYIDQGILATDQTDASS 322
           P G   EIM  RPRSDLY NLPAL+KLD ML+D+LD+F D+EF Y D+GI+  D  D  S
Sbjct: 187 PGGGTYEIMETRPRSDLYANLPALKKLDAMLIDMLDAFSDTEFWYTDRGIVLGD-CDKDS 246

Query: 323 SFRKLLERQDDKWWLPIPRVPNGGLSEASTRHLQHKRDCTNQILKAAMAINSVTLADMDV 382
                  RQ+DKWWLP P+VP  GLSE + + LQ  RD  NQILKAA+AINS  LA+M++
Sbjct: 247 YNSPASVRQEDKWWLPCPKVPPNGLSEEARKKLQQCRDFANQILKAALAINSGVLAEMEI 306

Query: 383 PISYLEGLPKNGRASLGEAIYKYISSDEFSPALLLECLDISSEHQAIEIANRVESAIYAW 442
           P  YLE LPK+G+  LGE IY+Y+++++FSP  LL+CLD+SSEHQ +EIANR+E+A++ W
Sbjct: 307 PDPYLETLPKSGKECLGEIIYQYLTANKFSPECLLDCLDLSSEHQTLEIANRIEAAVHVW 366

Query: 443 RTKGIASNNSK-----SSW-EMLKELMIDADKSEVLAERAELVLLCLKQQFPNLPQTSLD 502
           R K    +  +     SSW   +K L+ D ++++ L +RAE +L  L+ +FP LPQT+LD
Sbjct: 367 RQKNGRRHKKQAKLKLSSWGGKVKGLVNDNERNDFLVQRAETLLQSLRIRFPGLPQTTLD 426

Query: 503 MSKIQYNKDVGKAILESYSRVLESLAYNIVARIEDLLYVNELTKHSDQIPGISQLGIVAH 562
           M+KIQYNKDVG++ILESYSRV+ES+A+NI ARI+D+LYV++  + S     IS    ++ 
Sbjct: 427 MNKIQYNKDVGQSILESYSRVMESMAFNITARIDDVLYVDDAMRRS-----ISVTESLSL 486

Query: 563 NSSNRIHISMPFS--TSPCNTNFIKPSFSSVDLVGTRSPIPKPP 592
            S N ++    FS  +SP  + F  P+ S    V +RSP   PP
Sbjct: 487 FSINGLNPQKAFSVQSSPHGSPFATPALS----VASRSPRRAPP 507

BLAST of CSPI03G31850 vs. TAIR 10
Match: AT4G00460.2 (RHO guanyl-nucleotide exchange factor 3 )

HSP 1 Score: 408.3 bits (1048), Expect = 1.1e-113
Identity = 227/429 (52.91%), Postives = 316/429 (73.66%), Query Frame = 0

Query: 123 SSSSSSSSSSSSSSSSSSTSAASMGWPL-----HETVVEGEE----DRVVEREVSTDSET 182
           S ++S  S     ++S  + A+   WP+         + G E    + +V +E+S + E 
Sbjct: 47  SETTSGFSDQIDETNSFCSEASPCNWPVLTESKSSKCLSGLEMQSNECLVVQEIS-EPEL 106

Query: 183 EMMKERFSKLLLGEDMSGSGNGVCTALAISNAITNLCGSVFGQLWRLEPLEAERKAMWGR 242
           E MKERF+KLLLGEDMSGSG GVCTA+ ISNAITNL  +VFGQ  RLEPLE E++A+W R
Sbjct: 107 ETMKERFAKLLLGEDMSGSGKGVCTAVTISNAITNLYATVFGQNLRLEPLETEKRALWKR 166

Query: 243 EMEFLLCVSNHIVELIPIWQTFPDGTKLEIMTCRPRSDLYVNLPALRKLDHMLLDILDSF 302
           EM  LL V ++IVE IP  Q   +G  +E+M  RPR+D+Y+NLPALRKLD ML++ LDSF
Sbjct: 167 EMNCLLSVCDYIVEFIPRCQNLSNGATVEVMESRPRADIYINLPALRKLDSMLMEALDSF 226

Query: 303 VDSEFCYIDQGILATDQTDASS-SFRK-LLERQDDKWWLPIPRVPNGGLSEASTRHLQHK 362
            ++EF Y ++G L+     +S+ SFRK +++R+++KWWLP+P VP+ GLS+ + + L++K
Sbjct: 227 QNTEFWYAEEGSLSMKSARSSTGSFRKVIVQRKEEKWWLPVPLVPSEGLSDKARKQLKNK 286

Query: 363 RDCTNQILKAAMAINSVTLADMDVPISYLEGLPKNGRASLGEAIYKYIS-SDEFSPALLL 422
           R+ TNQI KAAMAINS  L++M++P SY+  LPK G++S+G++IY+Y+S S  F P  LL
Sbjct: 287 RESTNQIHKAAMAINSSILSEMEIPDSYMTTLPKCGKSSVGDSIYRYMSGSGRFFPEQLL 346

Query: 423 ECLDISSEHQAIEIANRVESAIYAWRTKGIASNNSKSSWEMLKELMID---ADKSEVLAE 482
           +CL+ISSEH+A+++A+RVE+++Y WR K   S NSK+SW M+K+LM      DK+ V+AE
Sbjct: 347 DCLNISSEHEAVQLADRVEASMYTWRRKSCLS-NSKNSWNMVKDLMSTTERTDKNYVMAE 406

Query: 483 RAELVLLCLKQQFPNLPQTSLDMSKIQYNKDVGKAILESYSRVLESLAYNIVARIEDLLY 537
           RAE +L CLKQ++P L QTSLD+ KIQYNKDVGKA+LESYSRVLE LA+NIVA I+D+LY
Sbjct: 407 RAETLLFCLKQRYPELSQTSLDICKIQYNKDVGKAVLESYSRVLEGLAFNIVAWIDDVLY 466

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LZN07.4e-16064.34Rop guanine nucleotide exchange factor 7 OS=Arabidopsis thaliana OX=3702 GN=ROPG... [more]
F4K2954.7e-13852.86Rop guanine nucleotide exchange factor 5 OS=Arabidopsis thaliana OX=3702 GN=ROPG... [more]
Q9M0563.1e-11849.32Rop guanine nucleotide exchange factor 6 OS=Arabidopsis thaliana OX=3702 GN=ROPG... [more]
Q93ZY21.2e-11750.19Rop guanine nucleotide exchange factor 1 OS=Arabidopsis thaliana OX=3702 GN=ROPG... [more]
A4IJ271.5e-11252.91Rop guanine nucleotide exchange factor 3 OS=Arabidopsis thaliana OX=3702 GN=ROPG... [more]
Match NameE-valueIdentityDescription
A0A0A0LAD80.0e+0096.46PRONE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G733280 PE=4 ... [more]
A0A5A7TMM71.1e-30992.28Rop guanine nucleotide exchange factor 7-like OS=Cucumis melo var. makuwa OX=119... [more]
A0A1S3B5B21.1e-30992.28rop guanine nucleotide exchange factor 7-like OS=Cucumis melo OX=3656 GN=LOC1034... [more]
A0A5D3DP031.4e-29188.67Rop guanine nucleotide exchange factor 7-like OS=Cucumis melo var. makuwa OX=119... [more]
A0A6J1CWY56.6e-21269.27rop guanine nucleotide exchange factor 7-like isoform X2 OS=Momordica charantia ... [more]
Match NameE-valueIdentityDescription
XP_004137923.30.0e+0099.01rop guanine nucleotide exchange factor 7 isoform X1 [Cucumis sativus][more]
XP_011651877.20.0e+0098.68rop guanine nucleotide exchange factor 7 isoform X2 [Cucumis sativus][more]
XP_008442490.12.3e-30992.28PREDICTED: rop guanine nucleotide exchange factor 7-like [Cucumis melo] >KAA0044... [more]
TYK25010.13.0e-29188.67rop guanine nucleotide exchange factor 7-like [Cucumis melo var. makuwa][more]
XP_038905547.12.3e-28385.06rop guanine nucleotide exchange factor 7-like isoform X2 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT5G02010.15.3e-16164.34RHO guanyl-nucleotide exchange factor 7 [more]
AT5G05940.13.3e-13952.86ROP guanine nucleotide exchange factor 5 [more]
AT3G55660.12.2e-11949.32ROP (rho of plants) guanine nucleotide exchange factor 6 [more]
AT4G38430.18.5e-11950.19rho guanyl-nucleotide exchange factor 1 [more]
AT4G00460.21.1e-11352.91RHO guanyl-nucleotide exchange factor 3 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D1.20.58.2010PRONE domain, subdomain 1coord: 231..365
e-value: 4.2E-20
score: 74.0
coord: 1..230
e-value: 9.2E-101
score: 338.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 408..428
NoneNo IPR availablePANTHERPTHR33101:SF14ROP GUANINE NUCLEOTIDE EXCHANGE FACTOR 7coord: 1..412
IPR005512PRONE domainPFAMPF03759PRONEcoord: 1..359
e-value: 1.8E-173
score: 576.5
IPR005512PRONE domainPROSITEPS51334PRONEcoord: 1..365
score: 113.945457
IPR038937Rop guanine nucleotide exchange factorPANTHERPTHR33101ROP GUANINE NUCLEOTIDE EXCHANGE FACTOR 1coord: 1..412

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI03G31850.2CSPI03G31850.2mRNA
CSPI03G31850.1CSPI03G31850.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0050790 regulation of catalytic activity
molecular_function GO:0005085 guanyl-nucleotide exchange factor activity