CSPI03G31620 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI03G31620
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Ubiquitin thioesterase OTU1
Location	Chr3: 28558363 .. 28561440 (+)
RNA-Seq Expression	CSPI03G31620
Synteny	CSPI03G31620

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GCGTGTTCGAATATAATAGGCAAAGCAAAGTAGCGACTCTCTCTTCGTTCTTCTCCGATGGATATTATGGCGGAGAAGTCCCAAAATGGTGAGAAACCTCTACCAGTTTCTTCTTTCTTTATTCTTATGGTTCTTATTTCTTCCTTTATCTGATTTTCTTCCCTTATTAAGTTCGTTGTCTGTTTGTTCATCTTTGTAGTGTCAATTCTCGAGCAATTGCAACTAGGAATTGCTCGGTTTGAGCTCTCATCTTCTCCTGTAAGTTCAATTTCACCTTCGAACTCAGTTGCTTCTTCTTTCCCTTTGTCTATGAATCCCAGTTATCCACTCTTTGCTAAAATTGGACCCGTGCTGTAAGTTCATTCCTTCTCTTTTAATTTGTGGTAGAGCTTTTGAATGCCATTGTTTTTCCAACTTCATAAATTGATATTCTTAGGGGGATTGAATCGCCTGCATTGAGGAAAGCTGAGCAATACAAAGTTCAAAAAGTTACTGGAGATGGACGCTGCCTTTTCCGTGCGCTTGTAACCTTCATTTCCCTATTTATTCTGTTGTTCACTCCTGAACTGAACTTATTTTGTGATTTACTCTGTTTGTACTAATTAATGTTTCAAATTATCTAATTGTGTATTTATGATTTTGATAGCCATTTCATCTTCAGTTATATGTTTCTGGAAATCAGATCTGTTTGTTAATAGTTAATTTATTTTGCGATATATTTCTTATTATTATGATCACGTGTTTTTGCTTCTGAAATTTTACTCAACTTGTATGAAGTGGAAGACAAATAAATTGTTAGGAACAAGGGTATTCTTTAGAAACAGAAGATTAAAGGCAGTCATTTCATTCTTAATTACTGTCATAGTATTTGTGTTCTCGCTTACTGGATGCAGCAAAGGGAGTGGAAGCTAATTTTCTTGCCTATTAAACTAACTTCCTCCTGATAAAATCAGGCTAAAGGAATGGCTTTCAACAGGGGGATTCCTCTTCGTCCATTTGAGGAGAAAAATGATGCAGGTTAGCTCAGATATATCATCTCTAGTAGGTATATTGCTAGTCTAAACATATATTATTTTGGATAATTGAGTGCAGAACATAATGTTCAAGTTGATAGAATAAGCAGGAGGATTGTCCAAATATTTTGTTTGCTATAACAGCCAAATGTCTTTGGAAGGTGCTTCGGTTGTATGCTACGTATAACTCAGCATCAGAGCTAGAATCTTGAAAGAAAAAAAAAAAAAGAACTTCATACTTTTGTGGCTTATGAAGTGATGAAGTTTTTGATTAAATAGCTTGGCCAAAAAAAAACTGTCAAGAGGAAAATGATATACTACCTAAATTTGTAGCAATCGGTTTGTGCTATGTTATTTAATAAATGATTGATGCTGGTAGATTAAGCATGCAGATGATTTACGCATGGCCGTGAAAGAAGTCATTTGTGACAATGGCAAAGAAAAACGGCAGTATGAAGCAGCCCTCATAGCAATTACTGTAGAGGAGCCATTAGAACGGTTAGTTTGTTTGCCCATTACTTTTTCATTATGTGAAACTGTCCTTTTAGATTTTTGTAGTTTTTGCTTGCGGTCTGATAATGAGAGCTTCCAAAAGTAAATTTCAGCTATTGCCAACGAATTCGGACACCTGATTTCTGGGGAGGAGAGTCGGAGCTATTGGTTAGTTCTCTATTCCCTTCCACTAGCGCGTCTTCACAAGGAATACTCAGAATTTTATTTATTTCTTGTACATGTGTGCTTTATGTGTGAAGGTGCTCTTTGTTGAGATAATGAATCACGATCTGTATTAATCTCCATCAGGTATTGTCAAAGCTCTGCAAGCAACCCATCATTGTCTACATACCAGAGCATGAGGTATACTTTTATCAATGACCATAAAAGTGTTGGTTTGATAACTGAGGTTGATTATATCCTCTGATAGTTTCCTGGGTTATTAACAGTGTAGGAACAAGATTTGGTGGTGTTACCCTTTTTAAGTTTAACTTTGATAATTTTACTTAGTCTCATTTGATTACACTTTCATTTTTAGTTTCCTATTTTAAAAAACAAAACTAATATTTTTAGTTAAACTCTAAAAATAAAAACTAAAATTTTTAGCTATGTTTGGTAATCCTTTTGGTTTTTGGTTTTATAAAACCGTGCTTGCTTCACACAATTCCTTGATCGTGTTTCCATATTTTCTATGTAAACACACACGAATCTTTCTTTCCCCAAATTTAAAAAACAAAACCAAAATTTTAAAAATTATTATTTTTTCAGTTTGCAAAAGTTGAATTTTGAAAACACTAAACAATAAAACACACAAATCAATATATGAAAGTGGTGATTATAGTCTTAACTTAAAAAAAAAAAACAAAAAACAAAAACCAAAATGGTTGCGAAAGGCAAAGAGGGGCTTAGTTTTTAGATCTTGGCTTGGTTTTTAAAAAACGTGGGCAAAGAGTGGATAGCAAAATAAAGGAACTCAATATGCAGGGTTTTCAAACAAAAGATTTGTTAGATAAACATGTTTAATTCACTTCTGTTTACTTGGATTTTGGTTTTTGTACTTGATTTGCAGCACAGAATGGGTTGTCGGGGCTCTAGCTTTATTCCTATTGCAGAATATGGAGCTGAGTTCAAGGGAGGAAAGCCCAAGAAACCTGTGAGGTTGCTTTACAGCGGTAGAAACCACTACGATCTACTTGTTTGAGAATCTTATTTGAAGGCCATCTCATTCCCACCTTGTTTTTTTCGAGTAAAAATGGGTAGATAGATTTGAATCAGGAACGACTTGTTTTTCACACCCTTGTTACTGATGCTATCTAAACATAACAAAAGAAATTGAGGCATGGTTGACAGGGTACAATTAGCATTTCAACAATGGTAGGATAACCTCAACAACACAACAACCGAAGCTCTCTTGTGGGCAATCACTTTTTGTAAAAGCTGTTGCATTTAGTTTTAAAAAGTGTTGTATTTCGTGAGGATTAATGGGGAGAGTAATGCACTTTACATATAAATAATTGGACAAAGGACACTTTTAAAATGATTAGGTGAATAAGAGTACTATTTTTATTTATAT

mRNA sequence

GCGTGTTCGAATATAATAGGCAAAGCAAAGTAGCGACTCTCTCTTCGTTCTTCTCCGATGGATATTATGGCGGAGAAGTCCCAAAATGTGTCAATTCTCGAGCAATTGCAACTAGGAATTGCTCGGTTTGAGCTCTCATCTTCTCCTGTAAGTTCAATTTCACCTTCGAACTCAGTTGCTTCTTCTTTCCCTTTGTCTATGAATCCCAGTTATCCACTCTTTGCTAAAATTGGACCCGTGCTGGGGATTGAATCGCCTGCATTGAGGAAAGCTGAGCAATACAAAGTTCAAAAAGTTACTGGAGATGGACGCTGCCTTTTCCGTGCGCTTGCTAAAGGAATGGCTTTCAACAGGGGGATTCCTCTTCGTCCATTTGAGGAGAAAAATGATGCAGATGATTTACGCATGGCCGTGAAAGAAGTCATTTGTGACAATGGCAAAGAAAAACGGCAGTATGAAGCAGCCCTCATAGCAATTACTGTAGAGGAGCCATTAGAACGCTATTGCCAACGAATTCGGACACCTGATTTCTGGGGAGGAGAGTCGGAGCTATTGGTATTGTCAAAGCTCTGCAAGCAACCCATCATTGTCTACATACCAGAGCATGAGCACAGAATGGGTTGTCGGGGCTCTAGCTTTATTCCTATTGCAGAATATGGAGCTGAGTTCAAGGGAGGAAAGCCCAAGAAACCTGTGAGGTTGCTTTACAGCGGTAGAAACCACTACGATCTACTTGTTTGAGAATCTTATTTGAAGGCCATCTCATTCCCACCTTGTTTTTTTCGAGTAAAAATGGGTAGATAGATTTGAATCAGGAACGACTTGTTTTTCACACCCTTGTTACTGATGCTATCTAAACATAACAAAAGAAATTGAGGCATGGTTGACAGGGTACAATTAGCATTTCAACAATGGTAGGATAACCTCAACAACACAACAACCGAAGCTCTCTTGTGGGCAATCACTTTTTGTAAAAGCTGTTGCATTTAGTTTTAAAAAGTGTTGTATTTCGTGAGGATTAATGGGGAGAGTAATGCACTTTACATATAAATAATTGGACAAAGGACACTTTTAAAATGATTAGGTGAATAAGAGTACTATTTTTATTTATAT

Coding sequence (CDS)

ATGGATATTATGGCGGAGAAGTCCCAAAATGTGTCAATTCTCGAGCAATTGCAACTAGGAATTGCTCGGTTTGAGCTCTCATCTTCTCCTGTAAGTTCAATTTCACCTTCGAACTCAGTTGCTTCTTCTTTCCCTTTGTCTATGAATCCCAGTTATCCACTCTTTGCTAAAATTGGACCCGTGCTGGGGATTGAATCGCCTGCATTGAGGAAAGCTGAGCAATACAAAGTTCAAAAAGTTACTGGAGATGGACGCTGCCTTTTCCGTGCGCTTGCTAAAGGAATGGCTTTCAACAGGGGGATTCCTCTTCGTCCATTTGAGGAGAAAAATGATGCAGATGATTTACGCATGGCCGTGAAAGAAGTCATTTGTGACAATGGCAAAGAAAAACGGCAGTATGAAGCAGCCCTCATAGCAATTACTGTAGAGGAGCCATTAGAACGCTATTGCCAACGAATTCGGACACCTGATTTCTGGGGAGGAGAGTCGGAGCTATTGGTATTGTCAAAGCTCTGCAAGCAACCCATCATTGTCTACATACCAGAGCATGAGCACAGAATGGGTTGTCGGGGCTCTAGCTTTATTCCTATTGCAGAATATGGAGCTGAGTTCAAGGGAGGAAAGCCCAAGAAACCTGTGAGGTTGCTTTACAGCGGTAGAAACCACTACGATCTACTTGTTTGA

Protein sequence

MDIMAEKSQNVSILEQLQLGIARFELSSSPVSSISPSNSVASSFPLSMNPSYPLFAKIGPVLGIESPALRKAEQYKVQKVTGDGRCLFRALAKGMAFNRGIPLRPFEEKNDADDLRMAVKEVICDNGKEKRQYEAALIAITVEEPLERYCQRIRTPDFWGGESELLVLSKLCKQPIIVYIPEHEHRMGCRGSSFIPIAEYGAEFKGGKPKKPVRLLYSGRNHYDLLV*

Homology

[Close]

BLAST of CSPI03G31620 vs. ExPASy Swiss-Prot
Match: Q8GYW0 (OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 3 OS=Arabidopsis thaliana OX=3702 GN=OTU3 PE=1 SV=1)

HSP 1 Score: 265.8 bits (678), Expect = 4.6e-70
Identity = 144/232 (62.07%), Postives = 171/232 (73.71%), Query Frame = 0


Query: 6   EKSQNVSILEQLQLGIARFELSSSPVSSISPSNSVASSFPLSM-----NPSYPLFAKIGP 65
           + S N +ILEQL+ G ARFEL SSP +S+S S S ++S P S        SY  FA+I  
Sbjct: 4   KSSSNNNILEQLRNGFARFELVSSPTASVSDSIS-STSLPASFISTTKGNSYVFFARINS 63

Query: 66  VLGIESPALRKAEQYKVQKVTGDGRCLFRALAKGMAFNRGIPLRPFEEKNDADDLRMAVK 125
            +   SPA +K E+Y V +V GDGRCLFRAL KGMAFN+GI L P  E++DAD+LRMAVK
Sbjct: 64  SMN-RSPAAKKVEKYAVDRVKGDGRCLFRALVKGMAFNKGITLNPQRERDDADELRMAVK 123

Query: 126 EVICDNGKEKRQYEAALIAITVEEPLERYCQRIRTPDFWGGESELLVLSKLCKQPIIVYI 185
           EVIC++ KE+ +Y+ AL+AITV+E L+R+CQRI   DFWGGESELLVLSKLCKQPIIVYI
Sbjct: 124 EVICNDPKEREKYKEALVAITVDESLKRFCQRIGRHDFWGGESELLVLSKLCKQPIIVYI 183

Query: 186 PEHEH-RMGCRGSSFIPIAEYGAEFKGG-----KPKKPVRLLYSGRNHYDLL 227
           PEHEH R G  G  FIPI EYG+EF+GG       K  VRLLYSGRNHYDLL
Sbjct: 184 PEHEHGRGGGYGPGFIPIQEYGSEFRGGWGKGKTNKNVVRLLYSGRNHYDLL 233

[Close]

BLAST of CSPI03G31620 vs. ExPASy Swiss-Prot
Match: Q8LBZ4 (OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 4 OS=Arabidopsis thaliana OX=3702 GN=OTU4 PE=1 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 9.1e-18
Identity = 56/155 (36.13%), Postives = 79/155 (50.97%), Query Frame = 0


Query: 75  YKVQKVTGDGRCLFRALAKGMAFNRGIPLRPFE--EKNDADDLRMAVKEVICDNGKEKRQ 134
           Y +  + GDGRCLFR++A G     G  L P E  ++  AD+LR  V +      +E   
Sbjct: 168 YSIIGIPGDGRCLFRSVAHGFCLRSG-KLAPGEKMQRELADELRTRVADEFIQRRQETEW 227

Query: 135 YEAALIAITVEEPLERYCQRIRTPDFWGGESELLVLSKLCKQPIIVYIPEHEHRMGCRGS 194
           +        VE   + Y ++IR P  WGGE EL + S + + PI VY+ +       +  
Sbjct: 228 F--------VEGDFDTYVRQIRDPHVWGGEPELFMASHVLQMPITVYMKDD------KAG 287

Query: 195 SFIPIAEYGAEFKGGKPKKPVRLLYSGRNHYDLLV 228
             I IAEYG E+  GK   P+R+LY G  HYD L+
Sbjct: 288 GLISIAEYGQEY--GK-DDPIRVLYHGFGHYDALL 304

[Close]

BLAST of CSPI03G31620 vs. ExPASy Swiss-Prot
Match: P22856 (Putative ubiquitin thioesterase L96 OS=Tipula iridescent virus OX=10490 GN=L96 PE=3 SV=1)

HSP 1 Score: 54.7 bits (130), Expect = 1.6e-06
Identity = 46/153 (30.07%), Postives = 66/153 (43.14%), Query Frame = 0


Query: 27  SSSPVSSISPSNSVASSFPLSMNPSYPLFAKIGPVLGIESPALRKAEQYKVQKVTGDGRC 86
           S+SP  ++ PS +  SS  +   P  P +        +E  A R+   YKV  V GDG C
Sbjct: 560 SNSPSITVDPSVTPPSSVKIKKRPELPEYKGGNADRDLEILARRRG--YKVIPVKGDGNC 619

Query: 87  LFRALAKGMAFNRGIPLRPFEEKNDADDLRMAVKEVICDNGKEKRQYEAALIAITVEEP- 146
           LFRA+ K +  N+ I       K   +DLR  V   +  + +    Y   +       P 
Sbjct: 620 LFRAVGKSLRLNQNI-------KYSHEDLRAQVVTYLTSHKEFLEPYLEYVTESGDTTPQ 679

Query: 147 -----LERYCQRIRTPDFWGGESELLVLSKLCK 174
                +ERY + I  P  WG    L VLS++ K
Sbjct: 680 EYAKNVERYIKNISKPGTWGDFICLRVLSEILK 703

[Close]

BLAST of CSPI03G31620 vs. ExPASy TrEMBL
Match: A0A0A0LCX5 (Ubiquitin thioesterase OTU1 OS=Cucumis sativus OX=3659 GN=Csa_3G732560 PE=4 SV=1)

HSP 1 Score: 458.0 bits (1177), Expect = 2.3e-125
Identity = 227/227 (100.00%), Postives = 227/227 (100.00%), Query Frame = 0


Query: 1   MDIMAEKSQNVSILEQLQLGIARFELSSSPVSSISPSNSVASSFPLSMNPSYPLFAKIGP 60
           MDIMAEKSQNVSILEQLQLGIARFELSSSPVSSISPSNSVASSFPLSMNPSYPLFAKIGP
Sbjct: 1   MDIMAEKSQNVSILEQLQLGIARFELSSSPVSSISPSNSVASSFPLSMNPSYPLFAKIGP 60

Query: 61  VLGIESPALRKAEQYKVQKVTGDGRCLFRALAKGMAFNRGIPLRPFEEKNDADDLRMAVK 120
           VLGIESPALRKAEQYKVQKVTGDGRCLFRALAKGMAFNRGIPLRPFEEKNDADDLRMAVK
Sbjct: 61  VLGIESPALRKAEQYKVQKVTGDGRCLFRALAKGMAFNRGIPLRPFEEKNDADDLRMAVK 120

Query: 121 EVICDNGKEKRQYEAALIAITVEEPLERYCQRIRTPDFWGGESELLVLSKLCKQPIIVYI 180
           EVICDNGKEKRQYEAALIAITVEEPLERYCQRIRTPDFWGGESELLVLSKLCKQPIIVYI
Sbjct: 121 EVICDNGKEKRQYEAALIAITVEEPLERYCQRIRTPDFWGGESELLVLSKLCKQPIIVYI 180

Query: 181 PEHEHRMGCRGSSFIPIAEYGAEFKGGKPKKPVRLLYSGRNHYDLLV 228
           PEHEHRMGCRGSSFIPIAEYGAEFKGGKPKKPVRLLYSGRNHYDLLV
Sbjct: 181 PEHEHRMGCRGSSFIPIAEYGAEFKGGKPKKPVRLLYSGRNHYDLLV 227

[Close]

BLAST of CSPI03G31620 vs. ExPASy TrEMBL
Match: A0A5D3DN03 (Ubiquitin thioesterase OTU1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G001090 PE=4 SV=1)

HSP 1 Score: 435.6 bits (1119), Expect = 1.2e-118
Identity = 215/227 (94.71%), Postives = 219/227 (96.48%), Query Frame = 0


Query: 1   MDIMAEKSQNVSILEQLQLGIARFELSSSPVSSISPSNSVASSFPLSMNPSYPLFAKIGP 60
           MDIMAEK +N SILEQLQLGIARFE SSSPVSSISPSNSVASSFPLSMN  +PLFAKIGP
Sbjct: 1   MDIMAEKPRNASILEQLQLGIARFEFSSSPVSSISPSNSVASSFPLSMNRGHPLFAKIGP 60

Query: 61  VLGIESPALRKAEQYKVQKVTGDGRCLFRALAKGMAFNRGIPLRPFEEKNDADDLRMAVK 120
           VLGIESPALRKAEQYKVQKVTGDGRCLFRALAKGMAFNRGIPLRPFEEKNDADDLRMAVK
Sbjct: 61  VLGIESPALRKAEQYKVQKVTGDGRCLFRALAKGMAFNRGIPLRPFEEKNDADDLRMAVK 120

Query: 121 EVICDNGKEKRQYEAALIAITVEEPLERYCQRIRTPDFWGGESELLVLSKLCKQPIIVYI 180
           EVICDNGKE+RQYE ALIAITVEEPLERYCQRIRTPDFWGGESELLVLSKLCKQPIIVYI
Sbjct: 121 EVICDNGKERRQYEEALIAITVEEPLERYCQRIRTPDFWGGESELLVLSKLCKQPIIVYI 180

Query: 181 PEHEHRMGCRGSSFIPIAEYGAEFKGGKPKKPVRLLYSGRNHYDLLV 228
           P HEH+MGCRG SFIPIAEYGAEFKGGKPKKPVRLLYSGRNHYDLLV
Sbjct: 181 PGHEHKMGCRGHSFIPIAEYGAEFKGGKPKKPVRLLYSGRNHYDLLV 227

[Close]

BLAST of CSPI03G31620 vs. ExPASy TrEMBL
Match: A0A1S3B5R5 (Ubiquitin thioesterase OTU1 OS=Cucumis melo OX=3656 GN=LOC103486327 PE=4 SV=1)

HSP 1 Score: 435.6 bits (1119), Expect = 1.2e-118
Identity = 215/227 (94.71%), Postives = 219/227 (96.48%), Query Frame = 0


Query: 1   MDIMAEKSQNVSILEQLQLGIARFELSSSPVSSISPSNSVASSFPLSMNPSYPLFAKIGP 60
           MDIMAEK +N SILEQLQLGIARFE SSSPVSSISPSNSVASSFPLSMN  +PLFAKIGP
Sbjct: 1   MDIMAEKPRNASILEQLQLGIARFEFSSSPVSSISPSNSVASSFPLSMNRGHPLFAKIGP 60

Query: 61  VLGIESPALRKAEQYKVQKVTGDGRCLFRALAKGMAFNRGIPLRPFEEKNDADDLRMAVK 120
           VLGIESPALRKAEQYKVQKVTGDGRCLFRALAKGMAFNRGIPLRPFEEKNDADDLRMAVK
Sbjct: 61  VLGIESPALRKAEQYKVQKVTGDGRCLFRALAKGMAFNRGIPLRPFEEKNDADDLRMAVK 120

Query: 121 EVICDNGKEKRQYEAALIAITVEEPLERYCQRIRTPDFWGGESELLVLSKLCKQPIIVYI 180
           EVICDNGKE+RQYE ALIAITVEEPLERYCQRIRTPDFWGGESELLVLSKLCKQPIIVYI
Sbjct: 121 EVICDNGKERRQYEEALIAITVEEPLERYCQRIRTPDFWGGESELLVLSKLCKQPIIVYI 180

Query: 181 PEHEHRMGCRGSSFIPIAEYGAEFKGGKPKKPVRLLYSGRNHYDLLV 228
           P HEH+MGCRG SFIPIAEYGAEFKGGKPKKPVRLLYSGRNHYDLLV
Sbjct: 181 PGHEHKMGCRGHSFIPIAEYGAEFKGGKPKKPVRLLYSGRNHYDLLV 227

[Close]

BLAST of CSPI03G31620 vs. ExPASy TrEMBL
Match: A0A6J1J705 (Ubiquitin thioesterase OTU1 OS=Cucurbita maxima OX=3661 GN=LOC111481933 PE=4 SV=1)

HSP 1 Score: 416.0 bits (1068), Expect = 1.0e-112
Identity = 206/227 (90.75%), Postives = 214/227 (94.27%), Query Frame = 0


Query: 1   MDIMAEKSQNVSILEQLQLGIARFELSSSPVSSISPSNSVASSFPLSMNPSYPLFAKIGP 60
           MDIMAEK QNV+ILEQL+LGIARFEL SSPV+SISPSNSVA SFP SMN   PLFAKIGP
Sbjct: 1   MDIMAEKPQNVAILEQLRLGIARFELVSSPVNSISPSNSVAPSFPFSMNRGRPLFAKIGP 60

Query: 61  VLGIESPALRKAEQYKVQKVTGDGRCLFRALAKGMAFNRGIPLRPFEEKNDADDLRMAVK 120
           +L  ESPALRKAE YKVQKVTGDGRCLFRALAKGMAFN+GIPLRPFEEKNDADDLRMAVK
Sbjct: 61  MLRAESPALRKAEHYKVQKVTGDGRCLFRALAKGMAFNKGIPLRPFEEKNDADDLRMAVK 120

Query: 121 EVICDNGKEKRQYEAALIAITVEEPLERYCQRIRTPDFWGGESELLVLSKLCKQPIIVYI 180
           EVICDNGKE+RQYE ALIAITVEEPLERYC+RIRTP+FWGGESELLVLSKLCKQPIIVYI
Sbjct: 121 EVICDNGKERRQYEEALIAITVEEPLERYCRRIRTPNFWGGESELLVLSKLCKQPIIVYI 180

Query: 181 PEHEHRMGCRGSSFIPIAEYGAEFKGGKPKKPVRLLYSGRNHYDLLV 228
           PEHEHRMG R SSFIPIAEYGAEFKGGKPKKPVRLLYSGRNHYDLLV
Sbjct: 181 PEHEHRMGSRSSSFIPIAEYGAEFKGGKPKKPVRLLYSGRNHYDLLV 227

[Close]

BLAST of CSPI03G31620 vs. ExPASy TrEMBL
Match: A0A6J1F6U0 (Ubiquitin thioesterase OTU1 OS=Cucurbita moschata OX=3662 GN=LOC111441401 PE=4 SV=1)

HSP 1 Score: 414.5 bits (1064), Expect = 2.9e-112
Identity = 205/227 (90.31%), Postives = 213/227 (93.83%), Query Frame = 0


Query: 1   MDIMAEKSQNVSILEQLQLGIARFELSSSPVSSISPSNSVASSFPLSMNPSYPLFAKIGP 60
           MDIMAEK QNV+ILEQL+LGIARFEL SSPV+SISPSNSVA SFP SMN   PLFAKIGP
Sbjct: 23  MDIMAEKPQNVAILEQLRLGIARFELVSSPVNSISPSNSVAPSFPFSMNRGRPLFAKIGP 82

Query: 61  VLGIESPALRKAEQYKVQKVTGDGRCLFRALAKGMAFNRGIPLRPFEEKNDADDLRMAVK 120
           +L  ESPALRKAE YKVQKVTGDGRCLFRALAKGMAFN+GIPLRPFEEKNDADDLRMAVK
Sbjct: 83  MLRAESPALRKAEHYKVQKVTGDGRCLFRALAKGMAFNKGIPLRPFEEKNDADDLRMAVK 142

Query: 121 EVICDNGKEKRQYEAALIAITVEEPLERYCQRIRTPDFWGGESELLVLSKLCKQPIIVYI 180
           EVICDNGKE+RQYE ALIAIT EEPLERYC+RIRTP+FWGGESELLVLSKLCKQPIIVYI
Sbjct: 143 EVICDNGKERRQYEEALIAITAEEPLERYCRRIRTPNFWGGESELLVLSKLCKQPIIVYI 202

Query: 181 PEHEHRMGCRGSSFIPIAEYGAEFKGGKPKKPVRLLYSGRNHYDLLV 228
           PEHEHRMG R SSFIPIAEYGAEFKGGKPKKPVRLLYSGRNHYDLLV
Sbjct: 203 PEHEHRMGSRSSSFIPIAEYGAEFKGGKPKKPVRLLYSGRNHYDLLV 249

[Close]

BLAST of CSPI03G31620 vs. NCBI nr
Match: XP_004137738.1 (OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 3 isoform X1 [Cucumis sativus] >KGN58799.1 hypothetical protein Csa_001241 [Cucumis sativus])

HSP 1 Score: 458.0 bits (1177), Expect = 4.8e-125
Identity = 227/227 (100.00%), Postives = 227/227 (100.00%), Query Frame = 0


Query: 1   MDIMAEKSQNVSILEQLQLGIARFELSSSPVSSISPSNSVASSFPLSMNPSYPLFAKIGP 60
           MDIMAEKSQNVSILEQLQLGIARFELSSSPVSSISPSNSVASSFPLSMNPSYPLFAKIGP
Sbjct: 1   MDIMAEKSQNVSILEQLQLGIARFELSSSPVSSISPSNSVASSFPLSMNPSYPLFAKIGP 60

Query: 61  VLGIESPALRKAEQYKVQKVTGDGRCLFRALAKGMAFNRGIPLRPFEEKNDADDLRMAVK 120
           VLGIESPALRKAEQYKVQKVTGDGRCLFRALAKGMAFNRGIPLRPFEEKNDADDLRMAVK
Sbjct: 61  VLGIESPALRKAEQYKVQKVTGDGRCLFRALAKGMAFNRGIPLRPFEEKNDADDLRMAVK 120

Query: 121 EVICDNGKEKRQYEAALIAITVEEPLERYCQRIRTPDFWGGESELLVLSKLCKQPIIVYI 180
           EVICDNGKEKRQYEAALIAITVEEPLERYCQRIRTPDFWGGESELLVLSKLCKQPIIVYI
Sbjct: 121 EVICDNGKEKRQYEAALIAITVEEPLERYCQRIRTPDFWGGESELLVLSKLCKQPIIVYI 180

Query: 181 PEHEHRMGCRGSSFIPIAEYGAEFKGGKPKKPVRLLYSGRNHYDLLV 228
           PEHEHRMGCRGSSFIPIAEYGAEFKGGKPKKPVRLLYSGRNHYDLLV
Sbjct: 181 PEHEHRMGCRGSSFIPIAEYGAEFKGGKPKKPVRLLYSGRNHYDLLV 227

[Close]

BLAST of CSPI03G31620 vs. NCBI nr
Match: XP_008442470.1 (PREDICTED: OTU domain-containing protein At3g57810 [Cucumis melo] >KAA0044146.1 OTU domain-containing protein [Cucumis melo var. makuwa] >TYK24991.1 OTU domain-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 435.6 bits (1119), Expect = 2.6e-118
Identity = 215/227 (94.71%), Postives = 219/227 (96.48%), Query Frame = 0


Query: 1   MDIMAEKSQNVSILEQLQLGIARFELSSSPVSSISPSNSVASSFPLSMNPSYPLFAKIGP 60
           MDIMAEK +N SILEQLQLGIARFE SSSPVSSISPSNSVASSFPLSMN  +PLFAKIGP
Sbjct: 1   MDIMAEKPRNASILEQLQLGIARFEFSSSPVSSISPSNSVASSFPLSMNRGHPLFAKIGP 60

Query: 61  VLGIESPALRKAEQYKVQKVTGDGRCLFRALAKGMAFNRGIPLRPFEEKNDADDLRMAVK 120
           VLGIESPALRKAEQYKVQKVTGDGRCLFRALAKGMAFNRGIPLRPFEEKNDADDLRMAVK
Sbjct: 61  VLGIESPALRKAEQYKVQKVTGDGRCLFRALAKGMAFNRGIPLRPFEEKNDADDLRMAVK 120

Query: 121 EVICDNGKEKRQYEAALIAITVEEPLERYCQRIRTPDFWGGESELLVLSKLCKQPIIVYI 180
           EVICDNGKE+RQYE ALIAITVEEPLERYCQRIRTPDFWGGESELLVLSKLCKQPIIVYI
Sbjct: 121 EVICDNGKERRQYEEALIAITVEEPLERYCQRIRTPDFWGGESELLVLSKLCKQPIIVYI 180

Query: 181 PEHEHRMGCRGSSFIPIAEYGAEFKGGKPKKPVRLLYSGRNHYDLLV 228
           P HEH+MGCRG SFIPIAEYGAEFKGGKPKKPVRLLYSGRNHYDLLV
Sbjct: 181 PGHEHKMGCRGHSFIPIAEYGAEFKGGKPKKPVRLLYSGRNHYDLLV 227

[Close]

BLAST of CSPI03G31620 vs. NCBI nr
Match: XP_038903455.1 (OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 3 isoform X1 [Benincasa hispida])

HSP 1 Score: 421.8 bits (1083), Expect = 3.8e-114
Identity = 210/227 (92.51%), Postives = 215/227 (94.71%), Query Frame = 0


Query: 1   MDIMAEKSQNVSILEQLQLGIARFELSSSPVSSISPSNSVASSFPLSMNPSYPLFAKIGP 60
           MDIMAEK QNV+ILEQLQLGIARFEL+SSPVSSISPSNSVA SFP SMN S+ LFAKIGP
Sbjct: 1   MDIMAEKPQNVAILEQLQLGIARFELASSPVSSISPSNSVAPSFPFSMNRSHSLFAKIGP 60

Query: 61  VLGIESPALRKAEQYKVQKVTGDGRCLFRALAKGMAFNRGIPLRPFEEKNDADDLRMAVK 120
           V   ESPALR AE YKVQKVTGDGRCLFRALAKGMAFNRGIPLRPFEEKNDADDLRMAVK
Sbjct: 61  VPDSESPALRNAEHYKVQKVTGDGRCLFRALAKGMAFNRGIPLRPFEEKNDADDLRMAVK 120

Query: 121 EVICDNGKEKRQYEAALIAITVEEPLERYCQRIRTPDFWGGESELLVLSKLCKQPIIVYI 180
           EVICDNGKE+RQYEAALIAITVEE LERYCQRIRTPDFWGGESELLVLSKLCKQPIIVYI
Sbjct: 121 EVICDNGKERRQYEAALIAITVEESLERYCQRIRTPDFWGGESELLVLSKLCKQPIIVYI 180

Query: 181 PEHEHRMGCRGSSFIPIAEYGAEFKGGKPKKPVRLLYSGRNHYDLLV 228
           PEHEH+MGCRGSSFIPIAEYGAEFK GKPKKPVRLLYSGRNHYDLLV
Sbjct: 181 PEHEHKMGCRGSSFIPIAEYGAEFKRGKPKKPVRLLYSGRNHYDLLV 227

[Close]

BLAST of CSPI03G31620 vs. NCBI nr
Match: XP_038903456.1 (OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 3 isoform X2 [Benincasa hispida])

HSP 1 Score: 417.9 bits (1073), Expect = 5.5e-113
Identity = 210/227 (92.51%), Postives = 215/227 (94.71%), Query Frame = 0


Query: 1   MDIMAEKSQNVSILEQLQLGIARFELSSSPVSSISPSNSVASSFPLSMNPSYPLFAKIGP 60
           MDIMAEK QNV+ILEQLQLGIARFEL+SSPVSSISPSNSVA SFP SMN S+ LFAKIGP
Sbjct: 1   MDIMAEKPQNVAILEQLQLGIARFELASSPVSSISPSNSVAPSFPFSMNRSHSLFAKIGP 60

Query: 61  VLGIESPALRKAEQYKVQKVTGDGRCLFRALAKGMAFNRGIPLRPFEEKNDADDLRMAVK 120
           V   ESPALR AE YKVQKVTGDGRCLFRALAKGMAFNRGIPLRPFEEKNDADDLRMAVK
Sbjct: 61  V--PESPALRNAEHYKVQKVTGDGRCLFRALAKGMAFNRGIPLRPFEEKNDADDLRMAVK 120

Query: 121 EVICDNGKEKRQYEAALIAITVEEPLERYCQRIRTPDFWGGESELLVLSKLCKQPIIVYI 180
           EVICDNGKE+RQYEAALIAITVEE LERYCQRIRTPDFWGGESELLVLSKLCKQPIIVYI
Sbjct: 121 EVICDNGKERRQYEAALIAITVEESLERYCQRIRTPDFWGGESELLVLSKLCKQPIIVYI 180

Query: 181 PEHEHRMGCRGSSFIPIAEYGAEFKGGKPKKPVRLLYSGRNHYDLLV 228
           PEHEH+MGCRGSSFIPIAEYGAEFK GKPKKPVRLLYSGRNHYDLLV
Sbjct: 181 PEHEHKMGCRGSSFIPIAEYGAEFKRGKPKKPVRLLYSGRNHYDLLV 225

[Close]

BLAST of CSPI03G31620 vs. NCBI nr
Match: XP_022983319.1 (OTU domain-containing protein At3g57810 [Cucurbita maxima])

HSP 1 Score: 416.0 bits (1068), Expect = 2.1e-112
Identity = 206/227 (90.75%), Postives = 214/227 (94.27%), Query Frame = 0


Query: 1   MDIMAEKSQNVSILEQLQLGIARFELSSSPVSSISPSNSVASSFPLSMNPSYPLFAKIGP 60
           MDIMAEK QNV+ILEQL+LGIARFEL SSPV+SISPSNSVA SFP SMN   PLFAKIGP
Sbjct: 1   MDIMAEKPQNVAILEQLRLGIARFELVSSPVNSISPSNSVAPSFPFSMNRGRPLFAKIGP 60

Query: 61  VLGIESPALRKAEQYKVQKVTGDGRCLFRALAKGMAFNRGIPLRPFEEKNDADDLRMAVK 120
           +L  ESPALRKAE YKVQKVTGDGRCLFRALAKGMAFN+GIPLRPFEEKNDADDLRMAVK
Sbjct: 61  MLRAESPALRKAEHYKVQKVTGDGRCLFRALAKGMAFNKGIPLRPFEEKNDADDLRMAVK 120

Query: 121 EVICDNGKEKRQYEAALIAITVEEPLERYCQRIRTPDFWGGESELLVLSKLCKQPIIVYI 180
           EVICDNGKE+RQYE ALIAITVEEPLERYC+RIRTP+FWGGESELLVLSKLCKQPIIVYI
Sbjct: 121 EVICDNGKERRQYEEALIAITVEEPLERYCRRIRTPNFWGGESELLVLSKLCKQPIIVYI 180

Query: 181 PEHEHRMGCRGSSFIPIAEYGAEFKGGKPKKPVRLLYSGRNHYDLLV 228
           PEHEHRMG R SSFIPIAEYGAEFKGGKPKKPVRLLYSGRNHYDLLV
Sbjct: 181 PEHEHRMGSRSSSFIPIAEYGAEFKGGKPKKPVRLLYSGRNHYDLLV 227

[Close]

BLAST of CSPI03G31620 vs. TAIR 10
Match: AT2G38025.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 265.8 bits (678), Expect = 3.3e-71
Identity = 144/232 (62.07%), Postives = 171/232 (73.71%), Query Frame = 0


Query: 6   EKSQNVSILEQLQLGIARFELSSSPVSSISPSNSVASSFPLSM-----NPSYPLFAKIGP 65
           + S N +ILEQL+ G ARFEL SSP +S+S S S ++S P S        SY  FA+I  
Sbjct: 4   KSSSNNNILEQLRNGFARFELVSSPTASVSDSIS-STSLPASFISTTKGNSYVFFARINS 63

Query: 66  VLGIESPALRKAEQYKVQKVTGDGRCLFRALAKGMAFNRGIPLRPFEEKNDADDLRMAVK 125
            +   SPA +K E+Y V +V GDGRCLFRAL KGMAFN+GI L P  E++DAD+LRMAVK
Sbjct: 64  SMN-RSPAAKKVEKYAVDRVKGDGRCLFRALVKGMAFNKGITLNPQRERDDADELRMAVK 123

Query: 126 EVICDNGKEKRQYEAALIAITVEEPLERYCQRIRTPDFWGGESELLVLSKLCKQPIIVYI 185
           EVIC++ KE+ +Y+ AL+AITV+E L+R+CQRI   DFWGGESELLVLSKLCKQPIIVYI
Sbjct: 124 EVICNDPKEREKYKEALVAITVDESLKRFCQRIGRHDFWGGESELLVLSKLCKQPIIVYI 183

Query: 186 PEHEH-RMGCRGSSFIPIAEYGAEFKGG-----KPKKPVRLLYSGRNHYDLL 227
           PEHEH R G  G  FIPI EYG+EF+GG       K  VRLLYSGRNHYDLL
Sbjct: 184 PEHEHGRGGGYGPGFIPIQEYGSEFRGGWGKGKTNKNVVRLLYSGRNHYDLL 233

[Close]

BLAST of CSPI03G31620 vs. TAIR 10
Match: AT3G57810.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 92.0 bits (227), Expect = 6.4e-19
Identity = 56/155 (36.13%), Postives = 79/155 (50.97%), Query Frame = 0


Query: 75  YKVQKVTGDGRCLFRALAKGMAFNRGIPLRPFE--EKNDADDLRMAVKEVICDNGKEKRQ 134
           Y +  + GDGRCLFR++A G     G  L P E  ++  AD+LR  V +      +E   
Sbjct: 125 YSIIGIPGDGRCLFRSVAHGFCLRSG-KLAPGEKMQRELADELRTRVADEFIQRRQETEW 184

Query: 135 YEAALIAITVEEPLERYCQRIRTPDFWGGESELLVLSKLCKQPIIVYIPEHEHRMGCRGS 194
           +        VE   + Y ++IR P  WGGE EL + S + + PI VY+ +       +  
Sbjct: 185 F--------VEGDFDTYVRQIRDPHVWGGEPELFMASHVLQMPITVYMKDD------KAG 244

Query: 195 SFIPIAEYGAEFKGGKPKKPVRLLYSGRNHYDLLV 228
             I IAEYG E+  GK   P+R+LY G  HYD L+
Sbjct: 245 GLISIAEYGQEY--GK-DDPIRVLYHGFGHYDALL 261

[Close]

BLAST of CSPI03G31620 vs. TAIR 10
Match: AT3G57810.2 (Cysteine proteinases superfamily protein )

HSP 1 Score: 92.0 bits (227), Expect = 6.4e-19
Identity = 56/155 (36.13%), Postives = 79/155 (50.97%), Query Frame = 0


Query: 75  YKVQKVTGDGRCLFRALAKGMAFNRGIPLRPFE--EKNDADDLRMAVKEVICDNGKEKRQ 134
           Y +  + GDGRCLFR++A G     G  L P E  ++  AD+LR  V +      +E   
Sbjct: 168 YSIIGIPGDGRCLFRSVAHGFCLRSG-KLAPGEKMQRELADELRTRVADEFIQRRQETEW 227

Query: 135 YEAALIAITVEEPLERYCQRIRTPDFWGGESELLVLSKLCKQPIIVYIPEHEHRMGCRGS 194
           +        VE   + Y ++IR P  WGGE EL + S + + PI VY+ +       +  
Sbjct: 228 F--------VEGDFDTYVRQIRDPHVWGGEPELFMASHVLQMPITVYMKDD------KAG 287

Query: 195 SFIPIAEYGAEFKGGKPKKPVRLLYSGRNHYDLLV 228
             I IAEYG E+  GK   P+R+LY G  HYD L+
Sbjct: 288 GLISIAEYGQEY--GK-DDPIRVLYHGFGHYDALL 304

[Close]

BLAST of CSPI03G31620 vs. TAIR 10
Match: AT3G57810.3 (Cysteine proteinases superfamily protein )

HSP 1 Score: 92.0 bits (227), Expect = 6.4e-19
Identity = 56/155 (36.13%), Postives = 79/155 (50.97%), Query Frame = 0


Query: 75  YKVQKVTGDGRCLFRALAKGMAFNRGIPLRPFE--EKNDADDLRMAVKEVICDNGKEKRQ 134
           Y +  + GDGRCLFR++A G     G  L P E  ++  AD+LR  V +      +E   
Sbjct: 168 YSIIGIPGDGRCLFRSVAHGFCLRSG-KLAPGEKMQRELADELRTRVADEFIQRRQETEW 227

Query: 135 YEAALIAITVEEPLERYCQRIRTPDFWGGESELLVLSKLCKQPIIVYIPEHEHRMGCRGS 194
           +        VE   + Y ++IR P  WGGE EL + S + + PI VY+ +       +  
Sbjct: 228 F--------VEGDFDTYVRQIRDPHVWGGEPELFMASHVLQMPITVYMKDD------KAG 287

Query: 195 SFIPIAEYGAEFKGGKPKKPVRLLYSGRNHYDLLV 228
             I IAEYG E+  GK   P+R+LY G  HYD L+
Sbjct: 288 GLISIAEYGQEY--GK-DDPIRVLYHGFGHYDALL 304

The following BLAST results are available for this feature:

BLAST of CSPI03G31620 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8GYW0	4.6e-70	62.07	OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 3 OS=Arabidopsis thalian...	[more]
Q8LBZ4	9.1e-18	36.13	OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 4 OS=Arabidopsis thalian...	[more]
P22856	1.6e-06	30.07	Putative ubiquitin thioesterase L96 OS=Tipula iridescent virus OX=10490 GN=L96 P...	[more]

BLAST of CSPI03G31620 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LCX5	2.3e-125	100.00	Ubiquitin thioesterase OTU1 OS=Cucumis sativus OX=3659 GN=Csa_3G732560 PE=4 SV=1	[more]
A0A5D3DN03	1.2e-118	94.71	Ubiquitin thioesterase OTU1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf...	[more]
A0A1S3B5R5	1.2e-118	94.71	Ubiquitin thioesterase OTU1 OS=Cucumis melo OX=3656 GN=LOC103486327 PE=4 SV=1	[more]
A0A6J1J705	1.0e-112	90.75	Ubiquitin thioesterase OTU1 OS=Cucurbita maxima OX=3661 GN=LOC111481933 PE=4 SV=...	[more]
A0A6J1F6U0	2.9e-112	90.31	Ubiquitin thioesterase OTU1 OS=Cucurbita moschata OX=3662 GN=LOC111441401 PE=4 S...	[more]

BLAST of CSPI03G31620 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004137738.1	4.8e-125	100.00	OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 3 isoform X1 [Cucumis sa...	[more]
XP_008442470.1	2.6e-118	94.71	PREDICTED: OTU domain-containing protein At3g57810 [Cucumis melo] >KAA0044146.1 ...	[more]
XP_038903455.1	3.8e-114	92.51	OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 3 isoform X1 [Benincasa ...	[more]
XP_038903456.1	5.5e-113	92.51	OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 3 isoform X2 [Benincasa ...	[more]
XP_022983319.1	2.1e-112	90.75	OTU domain-containing protein At3g57810 [Cucurbita maxima]	[more]

BLAST of CSPI03G31620 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G38025.1	3.3e-71	62.07	Cysteine proteinases superfamily protein	[more]
AT3G57810.1	6.4e-19	36.13	Cysteine proteinases superfamily protein	[more]
AT3G57810.2	6.4e-19	36.13	Cysteine proteinases superfamily protein	[more]
AT3G57810.3	6.4e-19	36.13	Cysteine proteinases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.90.70.80		coord: 59..227 e-value: 1.3E-35 score: 124.7
None	No IPR available	PANTHER	PTHR13312:SF3	OTU-LIKE CYSTEINE PROTEASE FAMILY PROTEIN, EXPRESSED	coord: 5..227
IPR003323	OTU domain	PFAM	PF02338	OTU	coord: 81..223 e-value: 3.8E-13 score: 50.0
IPR003323	OTU domain	PROSITE	PS50802	OTU	coord: 75..227 score: 15.851774
IPR039138	Ubiquitin thioesterase OTU1/2/3	PANTHER	PTHR13312	HIV-INDUCED PROTEIN-7-LIKE PROTEASE	coord: 5..227
IPR038765	Papain-like cysteine peptidase superfamily	SUPERFAMILY	54001	Cysteine proteinases	coord: 74..227

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G31620.1	CSPI03G31620.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030968	endoplasmic reticulum unfolded protein response
biological_process	GO:0016579	protein deubiquitination
biological_process	GO:0030433	ubiquitin-dependent ERAD pathway
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0004843	thiol-dependent deubiquitinase
molecular_function	GO:0101005	deubiquitinase activity