CSPI03G31610 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI03G31610
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Steroid 5-alpha-reductase DET2
Location	Chr3: 28557176 .. 28558261 (+)
RNA-Seq Expression	CSPI03G31610
Synteny	CSPI03G31610

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TCTCCATTTTTACCAAACTTTATTAAATGAAATTACTCGACAAAGTCACCAAAAAGTGTCCCAAGTCTATAACCACAGTGGAGAAGGTGACTTGACTATGAGAGTCGATAGGCTTCCTTTACTGTGTCACCTTCTTCCTTTTAGGTATGTAGATCTGGATTTTAATGAGACATGGTACAAATTTGAAAACAGATCCGCCACAGATTTCTCAACTTGATTCAAACCTACAACCGGAATCCTCGAATCCATCGCCTACTATGATGGGTTGGGATCAGGCTCTGTTCCACAACAGTCTTCTCGCCCTGTACCTCATTGGACCACCGACCGTCGTCTCCCTGAGGTTTCTCCAGGCGCCGTACGGGAAACACATCCGACCCGGGTGGGGCCCGACTATGTCGGCTCCATTGGCCTGGTTCCTCATGGAAAGTCCCACCTTATGGCTGACCCTCCTCCTCTTTCCTTCCGGCGATCACTCCTCCCATTCCAAGGCTCTCCTTCTCATCTCCCCCTTCCTCCTCCACTACCTCAATCGCACCTGCTTCTACCCTATTCGTCTTCTCTGGAGTGGAGCTCGACGGAACGCCCAAGCCGATTTTCCGGTGCTTGTGGCCATCATGGCATTCGGATTCAACTTGCTTAACTCTTACGTGCAGACCAGATCTGTGTCTCACTACGTAAAGTACGAGGGAGATGGGTGGTTTTGGTGGCGGTTTTTCGGTGGAGGAGTGGTTTTTGTGATGGGGATGGCGGTGAACATATGGTCGGATGGTGTACTGGTCGGACTGAAGAGGAGGGGCGGCGGTTATAAGGTGCCGACGGGAGGGTGGTTTGAGTTGGTGAGTTGTCCTAATTATTTGGGGGAGATTATGGAGTGGCTGGGCTGGGCGGTGATGTGCGGGTCATGGGCTGGGCTAGGGTTTTTCCTTTATACGTGTGCAAATTTAGTGCCTAGGGCACGTGCAAACCACACGTGGTACTTGAACAAGTTTGGAGAGGTTTATCCCATAAATAGGAAGGCTGTAATTCCCTTCTTGTATTAATGTCTTATCAAATTTCAATTTAAAATTAATAAATTCCTTTGCCTTA

mRNA sequence

TCTCCATTTTTACCAAACTTTATTAAATGAAATTACTCGACAAAGTCACCAAAAAGTGTCCCAAGTCTATAACCACAGTGGAGAAGGTGACTTGACTATGAGAGTCGATAGGCTTCCTTTACTGTGTCACCTTCTTCCTTTTAGGTATGTAGATCTGGATTTTAATGAGACATGGTACAAATTTGAAAACAGATCCGCCACAGATTTCTCAACTTGATTCAAACCTACAACCGGAATCCTCGAATCCATCGCCTACTATGATGGGTTGGGATCAGGCTCTGTTCCACAACAGTCTTCTCGCCCTGTACCTCATTGGACCACCGACCGTCGTCTCCCTGAGGTTTCTCCAGGCGCCGTACGGGAAACACATCCGACCCGGGTGGGGCCCGACTATGTCGGCTCCATTGGCCTGGTTCCTCATGGAAAGTCCCACCTTATGGCTGACCCTCCTCCTCTTTCCTTCCGGCGATCACTCCTCCCATTCCAAGGCTCTCCTTCTCATCTCCCCCTTCCTCCTCCACTACCTCAATCGCACCTGCTTCTACCCTATTCGTCTTCTCTGGAGTGGAGCTCGACGGAACGCCCAAGCCGATTTTCCGGTGCTTGTGGCCATCATGGCATTCGGATTCAACTTGCTTAACTCTTACGTGCAGACCAGATCTGTGTCTCACTACGTAAAGTACGAGGGAGATGGGTGGTTTTGGTGGCGGTTTTTCGGTGGAGGAGTGGTTTTTGTGATGGGGATGGCGGTGAACATATGGTCGGATGGTGTACTGGTCGGACTGAAGAGGAGGGGCGGCGGTTATAAGGTGCCGACGGGAGGGTGGTTTGAGTTGGTGAGTTGTCCTAATTATTTGGGGGAGATTATGGAGTGGCTGGGCTGGGCGGTGATGTGCGGGTCATGGGCTGGGCTAGGGTTTTTCCTTTATACGTGTGCAAATTTAGTGCCTAGGGCACGTGCAAACCACACGTGGTACTTGAACAAGTTTGGAGAGGTTTATCCCATAAATAGGAAGGCTGTAATTCCCTTCTTGTATTAATGTCTTATCAAATTTCAATTTAAAATTAATAAATTCCTTTGCCTTA

Coding sequence (CDS)

ATGAGACATGGTACAAATTTGAAAACAGATCCGCCACAGATTTCTCAACTTGATTCAAACCTACAACCGGAATCCTCGAATCCATCGCCTACTATGATGGGTTGGGATCAGGCTCTGTTCCACAACAGTCTTCTCGCCCTGTACCTCATTGGACCACCGACCGTCGTCTCCCTGAGGTTTCTCCAGGCGCCGTACGGGAAACACATCCGACCCGGGTGGGGCCCGACTATGTCGGCTCCATTGGCCTGGTTCCTCATGGAAAGTCCCACCTTATGGCTGACCCTCCTCCTCTTTCCTTCCGGCGATCACTCCTCCCATTCCAAGGCTCTCCTTCTCATCTCCCCCTTCCTCCTCCACTACCTCAATCGCACCTGCTTCTACCCTATTCGTCTTCTCTGGAGTGGAGCTCGACGGAACGCCCAAGCCGATTTTCCGGTGCTTGTGGCCATCATGGCATTCGGATTCAACTTGCTTAACTCTTACGTGCAGACCAGATCTGTGTCTCACTACGTAAAGTACGAGGGAGATGGGTGGTTTTGGTGGCGGTTTTTCGGTGGAGGAGTGGTTTTTGTGATGGGGATGGCGGTGAACATATGGTCGGATGGTGTACTGGTCGGACTGAAGAGGAGGGGCGGCGGTTATAAGGTGCCGACGGGAGGGTGGTTTGAGTTGGTGAGTTGTCCTAATTATTTGGGGGAGATTATGGAGTGGCTGGGCTGGGCGGTGATGTGCGGGTCATGGGCTGGGCTAGGGTTTTTCCTTTATACGTGTGCAAATTTAGTGCCTAGGGCACGTGCAAACCACACGTGGTACTTGAACAAGTTTGGAGAGGTTTATCCCATAAATAGGAAGGCTGTAATTCCCTTCTTGTATTAA

Protein sequence

MRHGTNLKTDPPQISQLDSNLQPESSNPSPTMMGWDQALFHNSLLALYLIGPPTVVSLRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTLLLFPSGDHSSHSKALLLISPFLLHYLNRTCFYPIRLLWSGARRNAQADFPVLVAIMAFGFNLLNSYVQTRSVSHYVKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKRRGGGYKVPTGGWFELVSCPNYLGEIMEWLGWAVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEVYPINRKAVIPFLY*

Homology

[Close]

BLAST of CSPI03G31610 vs. ExPASy Swiss-Prot
Match: Q2QDF6 (Steroid 5-alpha-reductase DET2 OS=Gossypium hirsutum OX=3635 GN=DET2 PE=1 SV=1)

HSP 1 Score: 358.2 bits (918), Expect = 8.7e-98
Identity = 176/262 (67.18%), Postives = 196/262 (74.81%), Query Frame = 0


Query: 33  MGWDQALFHNSLLALYLIGPPTVVSLRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPTLW 92
           M  DQ LFH  LL LY+I  PT +SL FLQAPYGKH RPGWGPT+S PLAWFLMESPTLW
Sbjct: 1   MASDQTLFHYCLLTLYIIALPTWISLYFLQAPYGKHNRPGWGPTISPPLAWFLMESPTLW 60

Query: 93  LTLLLFPSGDHSSHSKALLLISPFLLHYLNRTCFYPIRLLWSGARRNAQA-DFPVLVAIM 152
           LT  LFPSG H  + K+ LLISPFL HY NRT  YP+RL    AR   Q   FPV VA M
Sbjct: 61  LTFFLFPSGQHFYNPKSFLLISPFLFHYFNRTVLYPLRL----ARNTTQTRGFPVSVAFM 120

Query: 153 AFGFNLLNSYVQTRSVSHYV-KYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKRR 212
           AFGFNLLN Y+Q R VSHY   YE +  FWWRF  G ++FV+GM VN+ +D VLVGLK++
Sbjct: 121 AFGFNLLNGYLQARWVSHYKDDYENEELFWWRFLAGLLIFVVGMWVNVRADKVLVGLKKQ 180

Query: 213 G-GGYKVPTGGWFELVSCPNYLGEIMEWLGWAVMCGSWAGLGFFLYTCANLVPRARANHT 272
           G GGYK+P GG FELVSCPNY GEIMEW GWAVM  SW G GFFLYTCANL+PRARA   
Sbjct: 181 GDGGYKIPRGGLFELVSCPNYFGEIMEWFGWAVMTWSWVGFGFFLYTCANLMPRARATRL 240

Query: 273 WYLNKFGEVYPINRKAVIPFLY 292
           WYL KF + YP +RKAVIPF+Y
Sbjct: 241 WYLEKFKDDYPKDRKAVIPFIY 258

[Close]

BLAST of CSPI03G31610 vs. ExPASy Swiss-Prot
Match: Q38944 (Steroid 5-alpha-reductase DET2 OS=Arabidopsis thaliana OX=3702 GN=DET2 PE=1 SV=2)

HSP 1 Score: 342.0 bits (876), Expect = 6.4e-93
Identity = 164/260 (63.08%), Postives = 191/260 (73.46%), Query Frame = 0


Query: 36  DQALFHNSLLALYLIGPPTVVSLRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTL 95
           D+  F   LL L   GPPT V L+FLQAPYGKH R GWGPT+S P+AWF+MESPTLWLTL
Sbjct: 6   DKTFFRYCLLTLIFAGPPTAVLLKFLQAPYGKHNRTGWGPTVSPPIAWFVMESPTLWLTL 65

Query: 96  LLFPSGDHSSHSKALLLISPFLLHYLNRTCFYPIRLLWSG--ARRNAQADFPVLVAIMAF 155
           LLFP G H+ + K+LLL SP+L+HY +RT  YP+RL  S   A +N    FP+ +A +AF
Sbjct: 66  LLFPFGRHALNPKSLLLFSPYLIHYFHRTIIYPLRLFRSSFPAGKNG---FPITIAALAF 125

Query: 156 GFNLLNSYVQTRSVSHYV-KYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKRRG- 215
            FNLLN Y+Q R VSHY   YE   WFWWRF  G VVF+ GM +NI SD  LV LK+   
Sbjct: 126 TFNLLNGYIQARWVSHYKDDYEDGNWFWWRFVIGMVVFITGMYINITSDRTLVRLKKENR 185

Query: 216 GGYKVPTGGWFELVSCPNYLGEIMEWLGWAVMCGSWAGLGFFLYTCANLVPRARANHTWY 275
           GGY +P GGWFELVSCPNY GE +EWLGWAVM  SWAG+GFFLYTC+NL PRARA+H WY
Sbjct: 186 GGYVIPRGGWFELVSCPNYFGEAIEWLGWAVMTWSWAGIGFFLYTCSNLFPRARASHKWY 245

Query: 276 LNKFGEVYPINRKAVIPFLY 292
           + KF E YP  RKAVIPF+Y
Sbjct: 246 IAKFKEEYPKTRKAVIPFVY 262

[Close]

BLAST of CSPI03G31610 vs. ExPASy Swiss-Prot
Match: Q5K2N1 (Steroid 5-alpha-reductase DET2 OS=Solanum lycopersicum OX=4081 GN=DET2 PE=1 SV=1)

HSP 1 Score: 311.6 bits (797), Expect = 9.3e-84
Identity = 149/261 (57.09%), Postives = 187/261 (71.65%), Query Frame = 0


Query: 32  MMGWDQALFHNSLLALYLIGPPTVVSLRFLQAPYGKHIRPG-WGPTMSAPLAWFLMESPT 91
           M   D+ LF+  +  + ++  PT +  +F  +PYGKH      G T+S P+AW  MESPT
Sbjct: 1   MFSSDENLFNFIVFFILVMAFPTFILCQFFTSPYGKHYTSADSGTTISPPIAWAFMESPT 60

Query: 92  LWLTLLLFPSGDHSSHSKALLLISPFLLHYLNRTCFYPIRLLWSGARRNAQADFPVLVAI 151
           LWLT+++F  G + ++  A LLISP+L HY NRT  YP+RL      RN + +FP+ +A+
Sbjct: 61  LWLTIIVFRLGKNYTNPLAFLLISPYLFHYTNRTIIYPLRL----RSRNTKNNFPLNIAV 120

Query: 152 MAFGFNLLNSYVQTRSVSHYVKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKRR 211
            AF FNLLN+Y+Q+R VSHY  Y+ D WFW RF  G V+F  GM +NIW+DGVL+GLK +
Sbjct: 121 TAFIFNLLNAYIQSRWVSHYANYQEDDWFWVRFGIGLVIFGSGMLLNIWADGVLLGLKSQ 180

Query: 212 GGGYKVPTGGWFELVSCPNYLGEIMEWLGWAVMCGSWAGLGFFLYTCANLVPRARANHTW 271
           GGGYK+P GG F+ VS PNYLGEIMEWLGWA+M  SWAGL FF+YTCANLVPRA +NH W
Sbjct: 181 GGGYKIPRGGLFDYVSSPNYLGEIMEWLGWALMTWSWAGLAFFVYTCANLVPRAVSNHKW 240

Query: 272 YLNKFGEVYPINRKAVIPFLY 292
           YL KFGE YP NRKAV PFLY
Sbjct: 241 YLQKFGEDYPKNRKAVFPFLY 257

[Close]

BLAST of CSPI03G31610 vs. ExPASy Swiss-Prot
Match: P24008 (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Rattus norvegicus OX=10116 GN=Srd5a1 PE=1 SV=2)

HSP 1 Score: 146.4 bits (368), Expect = 5.2e-34
Identity = 90/241 (37.34%), Postives = 138/241 (57.26%), Query Frame = 0


Query: 54  TVVSLRFLQAPYGKHIRPGW-GPTMSAPLAWFLMESPTL-W-LTLLLFPSGDHSSHSKAL 113
           ++V LR + +PYG++  P W G  + A  AWF+ E P++ W L   + P+     +    
Sbjct: 27  SIVGLRSVGSPYGRY-SPQWPGIRVPARPAWFIQELPSMAWPLYEYIRPAAARLGNLPNR 86

Query: 114 LLISPFLLHYLNRTCFYPIRLLWSGARRNAQADFPVLVAIMAFGFNLLNSYVQTRSVSHY 173
           +L++ FL+HY+ RT  +P+  L  G +      F     ++AF F   N YVQ+R +S +
Sbjct: 87  VLLAMFLIHYVQRTLVFPV--LIRGGKPTLLVTF-----VLAFLFCTFNGYVQSRYLSQF 146

Query: 174 VKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKRRG-GGYKVPTGGWFELVSCPN 233
             Y  D      F  G  ++++GM +NI SD +L  L++ G  GYK+P GG FE VS  N
Sbjct: 147 AVYAEDWVTHPCFLTGFALWLVGMVINIHSDHILRNLRKPGETGYKIPRGGLFEYVSAAN 206

Query: 234 YLGEIMEWLGWAVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEVYPINRKAVIPF 291
           Y GE++EW G+A+   S  G+ F L+T + L+ RA+ +H WY  KF E YP +RK +IPF
Sbjct: 207 YFGELVEWCGFALASWSLQGVVFALFTLSTLLTRAKQHHQWYHEKF-EDYPKSRKILIPF 258

[Close]

BLAST of CSPI03G31610 vs. ExPASy Swiss-Prot
Match: Q28891 (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Macaca fascicularis OX=9541 GN=SRD5A1 PE=1 SV=1)

HSP 1 Score: 144.8 bits (364), Expect = 1.5e-33
Identity = 92/245 (37.55%), Postives = 127/245 (51.84%), Query Frame = 0


Query: 50  IGPPTVVSLRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTLLLFPSGDHSSHSKA 109
           +G   +   R     YG+H  P +   + A  AW + E P+L L L  + S        A
Sbjct: 27  VGCAVLARNRETNLAYGRHASPSFRVRVPARAAWVVQELPSLALPLYQYASESAPRLRSA 86

Query: 110 --LLLISPFLLHYLNRTCFYPIRLLWSGARRNAQADFPVLVAIMAFGFNLLNSYVQTRSV 169
              +L++ FL+HY +R   YP   L  G +       P+L   MA  F   N Y+Q+R +
Sbjct: 87  PNCILLAMFLVHYGHRCLIYP--FLMRGGK-----PMPLLACTMAIMFCTFNGYLQSRYL 146

Query: 170 SHYVKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKRRGG-GYKVPTGGWFELVS 229
           SH+  Y  D     RF  G  +++ GM +NI SD +L  L++ G  GYK+P GG FE V+
Sbjct: 147 SHWAVYADDWVTDPRFLIGFGLWLAGMLINIHSDHILRNLRKPGDTGYKIPRGGLFEYVT 206

Query: 230 CPNYLGEIMEWLGWAVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEVYPINRKAV 289
             NY GEIMEW G+A+   S  G  F  +T   L  RA+ +H WYL KF E YP  RK +
Sbjct: 207 AANYFGEIMEWCGYALASWSVQGAAFAFFTFCFLSGRAKEHHRWYLQKF-EEYPKFRKIL 263

Query: 290 IPFLY 292
           IPFL+
Sbjct: 267 IPFLF 263

[Close]

BLAST of CSPI03G31610 vs. ExPASy TrEMBL
Match: A0A0A0LAB3 (Steroid 5-alpha-reductase DET2 OS=Cucumis sativus OX=3659 GN=Csa_3G732550 PE=3 SV=1)

HSP 1 Score: 616.7 bits (1589), Expect = 5.0e-173
Identity = 288/291 (98.97%), Postives = 288/291 (98.97%), Query Frame = 0


Query: 1   MRHGTNLKTDPPQISQLDSNLQPESSNPSPTMMGWDQALFHNSLLALYLIGPPTVVSLRF 60
           MRHGTNLKTDPPQISQLDSNLQPESSNPSPTMMGWDQALFHNSLLALYLIGPPTVVSLRF
Sbjct: 1   MRHGTNLKTDPPQISQLDSNLQPESSNPSPTMMGWDQALFHNSLLALYLIGPPTVVSLRF 60

Query: 61  LQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTLLLFPSGDHSSHSKALLLISPFLLHY 120
           LQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTLLLFPSGDHSSHSKALLLISPFLLHY
Sbjct: 61  LQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTLLLFPSGDHSSHSKALLLISPFLLHY 120

Query: 121 LNRTCFYPIRLLWSGARRNAQADFPVLVAIMAFGFNLLNSYVQTRSVSHYVKYEGDGWFW 180
           LNRTCFYPIRLLWSG RRNAQ  FPVLVAIMAFGFNLLNSYVQTRSVSHYVKYEGDGWFW
Sbjct: 121 LNRTCFYPIRLLWSGTRRNAQVGFPVLVAIMAFGFNLLNSYVQTRSVSHYVKYEGDGWFW 180

Query: 181 WRFFGGGVVFVMGMAVNIWSDGVLVGLKRRGGGYKVPTGGWFELVSCPNYLGEIMEWLGW 240
           WRFFGGGVVFVMGMAVNIWSDGVLVGLKRRGGGYKVPTGGWFELVSCPNYLGEIMEWLGW
Sbjct: 181 WRFFGGGVVFVMGMAVNIWSDGVLVGLKRRGGGYKVPTGGWFELVSCPNYLGEIMEWLGW 240

Query: 241 AVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEVYPINRKAVIPFLY 292
           AVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEVYPINRKAVIPFLY
Sbjct: 241 AVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEVYPINRKAVIPFLY 291

[Close]

BLAST of CSPI03G31610 vs. ExPASy TrEMBL
Match: A0A1S3B6E3 (Steroid 5-alpha-reductase DET2 OS=Cucumis melo OX=3656 GN=LOC103486328 PE=3 SV=1)

HSP 1 Score: 588.2 bits (1515), Expect = 1.9e-164
Identity = 276/291 (94.85%), Postives = 280/291 (96.22%), Query Frame = 0


Query: 1   MRHGTNLKTDPPQISQLDSNLQPESSNPSPTMMGWDQALFHNSLLALYLIGPPTVVSLRF 60
           MR+ TNLKTD PQISQLDSNLQPESSNPSPT+MGWDQALF NSLLALYLIGPPTVVSLRF
Sbjct: 1   MRYVTNLKTDQPQISQLDSNLQPESSNPSPTVMGWDQALFRNSLLALYLIGPPTVVSLRF 60

Query: 61  LQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTLLLFPSGDHSSHSKALLLISPFLLHY 120
           LQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTLLLFPSGDHSS SKALLLISPFLLHY
Sbjct: 61  LQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTLLLFPSGDHSSDSKALLLISPFLLHY 120

Query: 121 LNRTCFYPIRLLWSGARRNAQADFPVLVAIMAFGFNLLNSYVQTRSVSHYVKYEGDGWFW 180
           LNRTCFYPIRLLWSG RRNA A FPV+VAIMAFGFNLLN+YVQTRSVSHYVKYEGDGWFW
Sbjct: 121 LNRTCFYPIRLLWSGTRRNATAGFPVIVAIMAFGFNLLNAYVQTRSVSHYVKYEGDGWFW 180

Query: 181 WRFFGGGVVFVMGMAVNIWSDGVLVGLKRRGGGYKVPTGGWFELVSCPNYLGEIMEWLGW 240
           WRFFGGGVVFVMGMAVNIWSDGVLVGLKR GGGYKVP GGWFELVSCPNYLGEIMEWLGW
Sbjct: 181 WRFFGGGVVFVMGMAVNIWSDGVLVGLKRSGGGYKVPKGGWFELVSCPNYLGEIMEWLGW 240

Query: 241 AVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEVYPINRKAVIPFLY 292
            VMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGE YPINRKAVIPFLY
Sbjct: 241 GVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEDYPINRKAVIPFLY 291

[Close]

BLAST of CSPI03G31610 vs. ExPASy TrEMBL
Match: A0A5D3DNY2 (Steroid 5-alpha-reductase DET2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G001080 PE=3 SV=1)

HSP 1 Score: 534.6 bits (1376), Expect = 2.5e-148
Identity = 248/259 (95.75%), Postives = 250/259 (96.53%), Query Frame = 0


Query: 33  MGWDQALFHNSLLALYLIGPPTVVSLRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPTLW 92
           MGWDQALF NSLLALYLIGPPTVVSLRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPTLW
Sbjct: 1   MGWDQALFRNSLLALYLIGPPTVVSLRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPTLW 60

Query: 93  LTLLLFPSGDHSSHSKALLLISPFLLHYLNRTCFYPIRLLWSGARRNAQADFPVLVAIMA 152
           LTLLLFPSGDHSS SKALLLISPFLLHYLNRTCFYPIRLLWSG RRNA A FPV+VAIMA
Sbjct: 61  LTLLLFPSGDHSSDSKALLLISPFLLHYLNRTCFYPIRLLWSGTRRNATAGFPVIVAIMA 120

Query: 153 FGFNLLNSYVQTRSVSHYVKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKRRGG 212
           FGFNLLN+YVQTRSVSHYVKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKR GG
Sbjct: 121 FGFNLLNAYVQTRSVSHYVKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKRSGG 180

Query: 213 GYKVPTGGWFELVSCPNYLGEIMEWLGWAVMCGSWAGLGFFLYTCANLVPRARANHTWYL 272
           GYKVP GGWFELVSCPNYLGEIMEWLGW VMCGSWAGLGFFLYTCANLVPRARANHTWYL
Sbjct: 181 GYKVPKGGWFELVSCPNYLGEIMEWLGWGVMCGSWAGLGFFLYTCANLVPRARANHTWYL 240

Query: 273 NKFGEVYPINRKAVIPFLY 292
           NKFGE YPINRKAVIPFLY
Sbjct: 241 NKFGEDYPINRKAVIPFLY 259

[Close]

BLAST of CSPI03G31610 vs. ExPASy TrEMBL
Match: A0A6J1J7F2 (Steroid 5-alpha-reductase DET2 OS=Cucurbita maxima OX=3661 GN=LOC111481932 PE=3 SV=1)

HSP 1 Score: 476.1 bits (1224), Expect = 1.1e-130
Identity = 222/259 (85.71%), Postives = 235/259 (90.73%), Query Frame = 0


Query: 33  MGWDQALFHNSLLALYLIGPPTVVSLRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPTLW 92
           M  DQ LFHNSLLALYLIGPPT +SLRFLQAPYGKH RPGWGPTMS PLAWFLMESPTLW
Sbjct: 1   MASDQTLFHNSLLALYLIGPPTAISLRFLQAPYGKHNRPGWGPTMSVPLAWFLMESPTLW 60

Query: 93  LTLLLFPSGDHSSHSKALLLISPFLLHYLNRTCFYPIRLLWSGARRNAQADFPVLVAIMA 152
           LTLLLFPSGD+SS+ K+LLLIS FLLHYLNRTC YPIRLL SG RRN+   FPV+VA+MA
Sbjct: 61  LTLLLFPSGDNSSNYKSLLLISLFLLHYLNRTCLYPIRLLRSGTRRNSATGFPVVVALMA 120

Query: 153 FGFNLLNSYVQTRSVSHYVKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKRRGG 212
           F FNLLNSY+QTRSVSHY+ Y+ D WFWWRFFGG VVFV+GMAVNIWSDGVLV LK  GG
Sbjct: 121 FVFNLLNSYLQTRSVSHYMDYDRDEWFWWRFFGGAVVFVLGMAVNIWSDGVLVKLKMAGG 180

Query: 213 GYKVPTGGWFELVSCPNYLGEIMEWLGWAVMCGSWAGLGFFLYTCANLVPRARANHTWYL 272
           GYKVPTGGWFELVSCPNYLGEI+EWLGWAVMCGSWAGLGFFLYTCANLVPRARANHTWYL
Sbjct: 181 GYKVPTGGWFELVSCPNYLGEIVEWLGWAVMCGSWAGLGFFLYTCANLVPRARANHTWYL 240

Query: 273 NKFGEVYPINRKAVIPFLY 292
           NKFGE YP +RKAVIPFLY
Sbjct: 241 NKFGEDYPRDRKAVIPFLY 259

[Close]

BLAST of CSPI03G31610 vs. ExPASy TrEMBL
Match: A0A6J1F1U8 (Steroid 5-alpha-reductase DET2 OS=Cucurbita moschata OX=3662 GN=LOC111441400 PE=3 SV=1)

HSP 1 Score: 473.4 bits (1217), Expect = 6.8e-130
Identity = 221/259 (85.33%), Postives = 234/259 (90.35%), Query Frame = 0


Query: 33  MGWDQALFHNSLLALYLIGPPTVVSLRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPTLW 92
           M  DQ LFHNSLLALYLIGPPT VSLRFLQAPYGKH RPGWGPTMS PLAWFLMESPTLW
Sbjct: 1   MASDQTLFHNSLLALYLIGPPTAVSLRFLQAPYGKHNRPGWGPTMSVPLAWFLMESPTLW 60

Query: 93  LTLLLFPSGDHSSHSKALLLISPFLLHYLNRTCFYPIRLLWSGARRNAQADFPVLVAIMA 152
           LTLLLFPSGD+SS+ K+LLLIS FLLHYLNRTC YPIRLL SG RRN+   FPV++A+MA
Sbjct: 61  LTLLLFPSGDNSSNYKSLLLISLFLLHYLNRTCLYPIRLLRSGTRRNSATGFPVVMALMA 120

Query: 153 FGFNLLNSYVQTRSVSHYVKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKRRGG 212
           F FNLLNSY+QTRSVSHY+ Y+ D WFWWRFFGG VVF++GMAVNIWSDGVLV LK  GG
Sbjct: 121 FVFNLLNSYLQTRSVSHYMDYDRDEWFWWRFFGGAVVFLLGMAVNIWSDGVLVKLKMAGG 180

Query: 213 GYKVPTGGWFELVSCPNYLGEIMEWLGWAVMCGSWAGLGFFLYTCANLVPRARANHTWYL 272
           GYKVPTGGWFELVSCPNYLGEI EWLGWAVMCGSWAGLGFFLYTCANLVPRARANHTWYL
Sbjct: 181 GYKVPTGGWFELVSCPNYLGEIAEWLGWAVMCGSWAGLGFFLYTCANLVPRARANHTWYL 240

Query: 273 NKFGEVYPINRKAVIPFLY 292
           NKFGE YP +RKAVIPFLY
Sbjct: 241 NKFGEDYPRDRKAVIPFLY 259

[Close]

BLAST of CSPI03G31610 vs. NCBI nr
Match: XP_004137737.1 (steroid 5-alpha-reductase DET2 [Cucumis sativus] >KGN58798.1 hypothetical protein Csa_002591 [Cucumis sativus])

HSP 1 Score: 616.7 bits (1589), Expect = 1.0e-172
Identity = 288/291 (98.97%), Postives = 288/291 (98.97%), Query Frame = 0


Query: 1   MRHGTNLKTDPPQISQLDSNLQPESSNPSPTMMGWDQALFHNSLLALYLIGPPTVVSLRF 60
           MRHGTNLKTDPPQISQLDSNLQPESSNPSPTMMGWDQALFHNSLLALYLIGPPTVVSLRF
Sbjct: 1   MRHGTNLKTDPPQISQLDSNLQPESSNPSPTMMGWDQALFHNSLLALYLIGPPTVVSLRF 60

Query: 61  LQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTLLLFPSGDHSSHSKALLLISPFLLHY 120
           LQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTLLLFPSGDHSSHSKALLLISPFLLHY
Sbjct: 61  LQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTLLLFPSGDHSSHSKALLLISPFLLHY 120

Query: 121 LNRTCFYPIRLLWSGARRNAQADFPVLVAIMAFGFNLLNSYVQTRSVSHYVKYEGDGWFW 180
           LNRTCFYPIRLLWSG RRNAQ  FPVLVAIMAFGFNLLNSYVQTRSVSHYVKYEGDGWFW
Sbjct: 121 LNRTCFYPIRLLWSGTRRNAQVGFPVLVAIMAFGFNLLNSYVQTRSVSHYVKYEGDGWFW 180

Query: 181 WRFFGGGVVFVMGMAVNIWSDGVLVGLKRRGGGYKVPTGGWFELVSCPNYLGEIMEWLGW 240
           WRFFGGGVVFVMGMAVNIWSDGVLVGLKRRGGGYKVPTGGWFELVSCPNYLGEIMEWLGW
Sbjct: 181 WRFFGGGVVFVMGMAVNIWSDGVLVGLKRRGGGYKVPTGGWFELVSCPNYLGEIMEWLGW 240

Query: 241 AVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEVYPINRKAVIPFLY 292
           AVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEVYPINRKAVIPFLY
Sbjct: 241 AVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEVYPINRKAVIPFLY 291

[Close]

BLAST of CSPI03G31610 vs. NCBI nr
Match: XP_008442471.1 (PREDICTED: steroid 5-alpha-reductase DET2 [Cucumis melo])

HSP 1 Score: 588.2 bits (1515), Expect = 3.9e-164
Identity = 276/291 (94.85%), Postives = 280/291 (96.22%), Query Frame = 0


Query: 1   MRHGTNLKTDPPQISQLDSNLQPESSNPSPTMMGWDQALFHNSLLALYLIGPPTVVSLRF 60
           MR+ TNLKTD PQISQLDSNLQPESSNPSPT+MGWDQALF NSLLALYLIGPPTVVSLRF
Sbjct: 1   MRYVTNLKTDQPQISQLDSNLQPESSNPSPTVMGWDQALFRNSLLALYLIGPPTVVSLRF 60

Query: 61  LQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTLLLFPSGDHSSHSKALLLISPFLLHY 120
           LQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTLLLFPSGDHSS SKALLLISPFLLHY
Sbjct: 61  LQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTLLLFPSGDHSSDSKALLLISPFLLHY 120

Query: 121 LNRTCFYPIRLLWSGARRNAQADFPVLVAIMAFGFNLLNSYVQTRSVSHYVKYEGDGWFW 180
           LNRTCFYPIRLLWSG RRNA A FPV+VAIMAFGFNLLN+YVQTRSVSHYVKYEGDGWFW
Sbjct: 121 LNRTCFYPIRLLWSGTRRNATAGFPVIVAIMAFGFNLLNAYVQTRSVSHYVKYEGDGWFW 180

Query: 181 WRFFGGGVVFVMGMAVNIWSDGVLVGLKRRGGGYKVPTGGWFELVSCPNYLGEIMEWLGW 240
           WRFFGGGVVFVMGMAVNIWSDGVLVGLKR GGGYKVP GGWFELVSCPNYLGEIMEWLGW
Sbjct: 181 WRFFGGGVVFVMGMAVNIWSDGVLVGLKRSGGGYKVPKGGWFELVSCPNYLGEIMEWLGW 240

Query: 241 AVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEVYPINRKAVIPFLY 292
            VMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGE YPINRKAVIPFLY
Sbjct: 241 GVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEDYPINRKAVIPFLY 291

[Close]

BLAST of CSPI03G31610 vs. NCBI nr
Match: KAA0044147.1 (steroid 5-alpha-reductase DET2 [Cucumis melo var. makuwa] >TYK24990.1 steroid 5-alpha-reductase DET2 [Cucumis melo var. makuwa])

HSP 1 Score: 534.6 bits (1376), Expect = 5.2e-148
Identity = 248/259 (95.75%), Postives = 250/259 (96.53%), Query Frame = 0


Query: 33  MGWDQALFHNSLLALYLIGPPTVVSLRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPTLW 92
           MGWDQALF NSLLALYLIGPPTVVSLRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPTLW
Sbjct: 1   MGWDQALFRNSLLALYLIGPPTVVSLRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPTLW 60

Query: 93  LTLLLFPSGDHSSHSKALLLISPFLLHYLNRTCFYPIRLLWSGARRNAQADFPVLVAIMA 152
           LTLLLFPSGDHSS SKALLLISPFLLHYLNRTCFYPIRLLWSG RRNA A FPV+VAIMA
Sbjct: 61  LTLLLFPSGDHSSDSKALLLISPFLLHYLNRTCFYPIRLLWSGTRRNATAGFPVIVAIMA 120

Query: 153 FGFNLLNSYVQTRSVSHYVKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKRRGG 212
           FGFNLLN+YVQTRSVSHYVKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKR GG
Sbjct: 121 FGFNLLNAYVQTRSVSHYVKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKRSGG 180

Query: 213 GYKVPTGGWFELVSCPNYLGEIMEWLGWAVMCGSWAGLGFFLYTCANLVPRARANHTWYL 272
           GYKVP GGWFELVSCPNYLGEIMEWLGW VMCGSWAGLGFFLYTCANLVPRARANHTWYL
Sbjct: 181 GYKVPKGGWFELVSCPNYLGEIMEWLGWGVMCGSWAGLGFFLYTCANLVPRARANHTWYL 240

Query: 273 NKFGEVYPINRKAVIPFLY 292
           NKFGE YPINRKAVIPFLY
Sbjct: 241 NKFGEDYPINRKAVIPFLY 259

[Close]

BLAST of CSPI03G31610 vs. NCBI nr
Match: XP_038905208.1 (LOW QUALITY PROTEIN: steroid 5-alpha-reductase DET2 [Benincasa hispida])

HSP 1 Score: 496.5 bits (1277), Expect = 1.6e-136
Identity = 230/259 (88.80%), Postives = 242/259 (93.44%), Query Frame = 0


Query: 33  MGWDQALFHNSLLALYLIGPPTVVSLRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPTLW 92
           MG DQALFHNSLL LYLIGP  VV+LRFLQAPYGKH +PGWGPTMSAPLAWFLMESPTLW
Sbjct: 1   MGSDQALFHNSLLRLYLIGPRQVVALRFLQAPYGKHNQPGWGPTMSAPLAWFLMESPTLW 60

Query: 93  LTLLLFPSGDHSSHSKALLLISPFLLHYLNRTCFYPIRLLWSGARRNAQADFPVLVAIMA 152
           LTLLLFPSGDHSS+SKALLLISPFLLHY NRTC YPIRLL SG RRN  A FPV+VA+MA
Sbjct: 61  LTLLLFPSGDHSSNSKALLLISPFLLHYFNRTCLYPIRLLRSGTRRNTAAGFPVIVAVMA 120

Query: 153 FGFNLLNSYVQTRSVSHYVKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKRRGG 212
           FGFNLLNSY+QTRSVSHY++YEGDGWFWWRFFGGGVVF++GM+VNIWSDGVLVGLKR GG
Sbjct: 121 FGFNLLNSYLQTRSVSHYMEYEGDGWFWWRFFGGGVVFLLGMSVNIWSDGVLVGLKRGGG 180

Query: 213 GYKVPTGGWFELVSCPNYLGEIMEWLGWAVMCGSWAGLGFFLYTCANLVPRARANHTWYL 272
           GYKVPTG WFELVSCPNYLGEI+EWLGWAVMCGSWAGL FFLYTCANLVPRARANHTWYL
Sbjct: 181 GYKVPTGWWFELVSCPNYLGEIVEWLGWAVMCGSWAGLAFFLYTCANLVPRARANHTWYL 240

Query: 273 NKFGEVYPINRKAVIPFLY 292
           NKFGE YP NRKAVIPFLY
Sbjct: 241 NKFGEDYPRNRKAVIPFLY 259

[Close]

BLAST of CSPI03G31610 vs. NCBI nr
Match: XP_023527474.1 (steroid 5-alpha-reductase DET2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 481.1 bits (1237), Expect = 6.8e-132
Identity = 225/259 (86.87%), Postives = 236/259 (91.12%), Query Frame = 0


Query: 33  MGWDQALFHNSLLALYLIGPPTVVSLRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPTLW 92
           M  DQ LFHNSLLALYLIGPPT VSLRFLQAPYGKH RPGWGPTMS PLAWFLMESPTLW
Sbjct: 1   MASDQTLFHNSLLALYLIGPPTAVSLRFLQAPYGKHNRPGWGPTMSVPLAWFLMESPTLW 60

Query: 93  LTLLLFPSGDHSSHSKALLLISPFLLHYLNRTCFYPIRLLWSGARRNAQADFPVLVAIMA 152
           LTLLLFPSGD+SS+ K+LLLIS FLLHYLNRTC YPIRLL SG RRN+   FPV+VA+MA
Sbjct: 61  LTLLLFPSGDNSSNYKSLLLISLFLLHYLNRTCLYPIRLLRSGTRRNSATGFPVVVALMA 120

Query: 153 FGFNLLNSYVQTRSVSHYVKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKRRGG 212
           FGFNLLNSY+QTRSVSHYV Y+ D WFWWRFFGG VVFV+GMAVNIWSDGVLV LK  GG
Sbjct: 121 FGFNLLNSYLQTRSVSHYVDYDRDEWFWWRFFGGAVVFVLGMAVNIWSDGVLVKLKMAGG 180

Query: 213 GYKVPTGGWFELVSCPNYLGEIMEWLGWAVMCGSWAGLGFFLYTCANLVPRARANHTWYL 272
           GYKVPTGGWFELVSCPNYLGEI+EWLGWAVMCGSWAGLGFFLYTCANLVPRARANHTWYL
Sbjct: 181 GYKVPTGGWFELVSCPNYLGEIVEWLGWAVMCGSWAGLGFFLYTCANLVPRARANHTWYL 240

Query: 273 NKFGEVYPINRKAVIPFLY 292
           NKFGE YP +RKAVIPFLY
Sbjct: 241 NKFGEDYPRDRKAVIPFLY 259

[Close]

BLAST of CSPI03G31610 vs. TAIR 10
Match: AT2G38050.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 342.0 bits (876), Expect = 4.6e-94
Identity = 164/260 (63.08%), Postives = 191/260 (73.46%), Query Frame = 0


Query: 36  DQALFHNSLLALYLIGPPTVVSLRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTL 95
           D+  F   LL L   GPPT V L+FLQAPYGKH R GWGPT+S P+AWF+MESPTLWLTL
Sbjct: 6   DKTFFRYCLLTLIFAGPPTAVLLKFLQAPYGKHNRTGWGPTVSPPIAWFVMESPTLWLTL 65

Query: 96  LLFPSGDHSSHSKALLLISPFLLHYLNRTCFYPIRLLWSG--ARRNAQADFPVLVAIMAF 155
           LLFP G H+ + K+LLL SP+L+HY +RT  YP+RL  S   A +N    FP+ +A +AF
Sbjct: 66  LLFPFGRHALNPKSLLLFSPYLIHYFHRTIIYPLRLFRSSFPAGKNG---FPITIAALAF 125

Query: 156 GFNLLNSYVQTRSVSHYV-KYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKRRG- 215
            FNLLN Y+Q R VSHY   YE   WFWWRF  G VVF+ GM +NI SD  LV LK+   
Sbjct: 126 TFNLLNGYIQARWVSHYKDDYEDGNWFWWRFVIGMVVFITGMYINITSDRTLVRLKKENR 185

Query: 216 GGYKVPTGGWFELVSCPNYLGEIMEWLGWAVMCGSWAGLGFFLYTCANLVPRARANHTWY 275
           GGY +P GGWFELVSCPNY GE +EWLGWAVM  SWAG+GFFLYTC+NL PRARA+H WY
Sbjct: 186 GGYVIPRGGWFELVSCPNYFGEAIEWLGWAVMTWSWAGIGFFLYTCSNLFPRARASHKWY 245

Query: 276 LNKFGEVYPINRKAVIPFLY 292
           + KF E YP  RKAVIPF+Y
Sbjct: 246 IAKFKEEYPKTRKAVIPFVY 262

[Close]

BLAST of CSPI03G31610 vs. TAIR 10
Match: AT5G16010.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 74.3 bits (181), Expect = 1.8e-13
Identity = 38/108 (35.19%), Postives = 67/108 (62.04%), Query Frame = 0


Query: 186 GGVVFVMGMAVNIWSDGVLVGLKRRGG--GYKVPTGGWFELVSCPNYLGEIMEWLGWAVM 245
           G V+FV+G+  N++   +L  L++  G   YK+P GG F+++ CP+YL EI+ +  + ++
Sbjct: 162 GVVMFVVGIVGNLYHHVLLAKLRKEDGKKEYKIPKGGLFDIIICPHYLFEILVFWSFFLI 221

Query: 246 CGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEVYPINRKAVIPFLY 292
             +     F + T   L+ R+ A  TWYL+KF + +P + KA+IPF++
Sbjct: 222 SQTIYSFSFAMGTMLYLIGRSYATRTWYLSKFDD-FPKHIKALIPFVF 268

[Close]

BLAST of CSPI03G31610 vs. TAIR 10
Match: AT1G72590.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 71.2 bits (173), Expect = 1.5e-12
Identity = 42/114 (36.84%), Postives = 57/114 (50.00%), Query Frame = 0


Query: 186 GGVVFVMGMAVNIWSDGVLVGLKR---RGGGYKVPTGGWFELVSCPNYLGEIMEWLGWAV 245
           GG +F+ G         +L  L+    +   Y +P G WFE+VSCP++L EI+ +LG  +
Sbjct: 211 GGAIFLWGWIHQRRCHAILGSLREYPSQAKEYIIPYGDWFEMVSCPHFLAEIVLYLGLLI 270

Query: 246 MCGS-----WAGLGFFLYTCANLVPRARANHTWYLNKFGEVYPINRKAVIPFLY 292
             G      W   GF     ANL   A   H WYL KF E YP +R A+ P +Y
Sbjct: 271 SSGGTDISIWLLFGF---VAANLTYAAGETHRWYLQKF-ENYPASRHAIFPHVY 320

[Close]

BLAST of CSPI03G31610 vs. TAIR 10
Match: AT2G16530.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 67.4 bits (163), Expect = 2.2e-11
Identity = 48/145 (33.10%), Postives = 66/145 (45.52%), Query Frame = 0


Query: 155 FNLLNSYVQTRSVSHYVKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKR---RG 214
           FNLL+      S+S  +K     W       GG +F+ G         +L  L+    + 
Sbjct: 215 FNLLS------SISPLMKLGSLQWI------GGAIFLWGWIHQRRCHAILGSLRENPSQA 274

Query: 215 GGYKVPTGGWFELVSCPNYLGEIMEWLGWAVMCGS-----WAGLGFFLYTCANLVPRARA 274
             Y +P G WF +VS P++L EI+ + G  +  G      W   GF     ANL   A  
Sbjct: 275 KEYIIPYGDWFGMVSSPHFLAEIVLYAGLLIASGGTDITIWLLFGF---VAANLTYAAGE 334

Query: 275 NHTWYLNKFGEVYPINRKAVIPFLY 292
            H WYL KF E YP NR A+ P++Y
Sbjct: 335 THRWYLRKF-ENYPANRHAIFPYVY 343

[Close]

BLAST of CSPI03G31610 vs. TAIR 10
Match: AT2G16530.2 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 67.4 bits (163), Expect = 2.2e-11
Identity = 48/145 (33.10%), Postives = 66/145 (45.52%), Query Frame = 0


Query: 155 FNLLNSYVQTRSVSHYVKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKR---RG 214
           FNLL+      S+S  +K     W       GG +F+ G         +L  L+    + 
Sbjct: 214 FNLLS------SISPLMKLGSLQWI------GGAIFLWGWIHQRRCHAILGSLRENPSQA 273

Query: 215 GGYKVPTGGWFELVSCPNYLGEIMEWLGWAVMCGS-----WAGLGFFLYTCANLVPRARA 274
             Y +P G WF +VS P++L EI+ + G  +  G      W   GF     ANL   A  
Sbjct: 274 KEYIIPYGDWFGMVSSPHFLAEIVLYAGLLIASGGTDITIWLLFGF---VAANLTYAAGE 333

Query: 275 NHTWYLNKFGEVYPINRKAVIPFLY 292
            H WYL KF E YP NR A+ P++Y
Sbjct: 334 THRWYLRKF-ENYPANRHAIFPYVY 342

The following BLAST results are available for this feature:

BLAST of CSPI03G31610 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q2QDF6	8.7e-98	67.18	Steroid 5-alpha-reductase DET2 OS=Gossypium hirsutum OX=3635 GN=DET2 PE=1 SV=1	[more]
Q38944	6.4e-93	63.08	Steroid 5-alpha-reductase DET2 OS=Arabidopsis thaliana OX=3702 GN=DET2 PE=1 SV=2	[more]
Q5K2N1	9.3e-84	57.09	Steroid 5-alpha-reductase DET2 OS=Solanum lycopersicum OX=4081 GN=DET2 PE=1 SV=1	[more]
P24008	5.2e-34	37.34	3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Rattus norvegicus OX=10116 GN=Srd5a1 ...	[more]
Q28891	1.5e-33	37.55	3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Macaca fascicularis OX=9541 GN=SRD5A1...	[more]

BLAST of CSPI03G31610 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LAB3	5.0e-173	98.97	Steroid 5-alpha-reductase DET2 OS=Cucumis sativus OX=3659 GN=Csa_3G732550 PE=3 S...	[more]
A0A1S3B6E3	1.9e-164	94.85	Steroid 5-alpha-reductase DET2 OS=Cucumis melo OX=3656 GN=LOC103486328 PE=3 SV=1	[more]
A0A5D3DNY2	2.5e-148	95.75	Steroid 5-alpha-reductase DET2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s...	[more]
A0A6J1J7F2	1.1e-130	85.71	Steroid 5-alpha-reductase DET2 OS=Cucurbita maxima OX=3661 GN=LOC111481932 PE=3 ...	[more]
A0A6J1F1U8	6.8e-130	85.33	Steroid 5-alpha-reductase DET2 OS=Cucurbita moschata OX=3662 GN=LOC111441400 PE=...	[more]

BLAST of CSPI03G31610 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004137737.1	1.0e-172	98.97	steroid 5-alpha-reductase DET2 [Cucumis sativus] >KGN58798.1 hypothetical protei...	[more]
XP_008442471.1	3.9e-164	94.85	PREDICTED: steroid 5-alpha-reductase DET2 [Cucumis melo]	[more]
KAA0044147.1	5.2e-148	95.75	steroid 5-alpha-reductase DET2 [Cucumis melo var. makuwa] >TYK24990.1 steroid 5-...	[more]
XP_038905208.1	1.6e-136	88.80	LOW QUALITY PROTEIN: steroid 5-alpha-reductase DET2 [Benincasa hispida]	[more]
XP_023527474.1	6.8e-132	86.87	steroid 5-alpha-reductase DET2 [Cucurbita pepo subsp. pepo]	[more]

BLAST of CSPI03G31610 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G38050.1	4.6e-94	63.08	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT5G16010.1	1.8e-13	35.19	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT1G72590.1	1.5e-12	36.84	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT2G16530.1	2.2e-11	33.10	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT2G16530.2	2.2e-11	33.10	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	1.20.120.1630		coord: 108..290 e-value: 1.0E-10 score: 43.7
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 7..29
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..29
None	No IPR available	PANTHER	PTHR10556:SF53	STEROID 5-ALPHA-REDUCTASE DET2	coord: 32..291
IPR001104	3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal	PFAM	PF02544	Steroid_dh	coord: 143..291 e-value: 7.0E-47 score: 159.2
IPR001104	3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal	PROSITE	PS50244	S5A_REDUCTASE	coord: 186..265 score: 26.575716
IPR016636	3-oxo-5-alpha-steroid 4-dehydrogenase	PIRSF	PIRSF015596	5_alpha-SR2	coord: 30..291 e-value: 9.2E-83 score: 275.4
IPR039357	3-oxo-5-alpha-steroid 4-dehydrogenase/very-long-chain enoyl-CoA reductase	PANTHER	PTHR10556	3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE	coord: 32..291

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G31610.1	CSPI03G31610.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016132	brassinosteroid biosynthetic process
biological_process	GO:0006629	lipid metabolic process
biological_process	GO:0008202	steroid metabolic process
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0016020	membrane
molecular_function	GO:0003865	3-oxo-5-alpha-steroid 4-dehydrogenase activity
molecular_function	GO:0047751	cholestenone 5-alpha-reductase activity
molecular_function	GO:0016627	oxidoreductase activity, acting on the CH-CH group of donors