CSPI03G31490 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI03G31490
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	AdoMet-dependent rRNA methyltransferase spb1
Location	Chr3: 28496091 .. 28500715 (+)
RNA-Seq Expression	CSPI03G31490
Synteny	CSPI03G31490

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAATACACTTAGACTTCCTTTGATGCGCAACTTTGACTTTCTTGCTGGGTTTGAATGTTGCCAGGAGGCCTGTCTGCTCCGCACTACCGGTAACTCTTGCTTGGTTCTTCAGTCATGGGCAAAGCTTCAAGAGATAAGAGGGTTTAAGCTCTATTCTATTTGACTATTTGTATTCCTGATTCATTATCTTTTACCATGTTTTGTTTACTTGGTCTCATTTGCAGGATATATACTACCGTAAAGCAAAAGAAGAAGGTTGGCGTGCTCGAAGTGCATTTAAACTTCTTCAGATAGACGAGGAATTTAACATATTTGAAGGTTTGTTCTTGTATTGAGCTCCTCTATTGAAATATTGTTACGTTTCTGGTAGGATGTCAATCACTGAGTTGGTCATGATAATTAATCTTTCTGCAACATTTTGGATCAACCTTTCTAACAGAGTTGTTTTGTGTGATCTTTATGACTTTGTAATCATCCTGTTTTATCATGTATAAGTATTTCGAAATGCTTCCAAGTACGTTGTCTACAATGAAATTTCATATGCAGGAGTCAAGAGAGTGGTAGATTTATGTGCAGCCCCCGGTAGCTGGAGCCAGGTAATTTCTTTTCAGCATCTTCTTGTTGCAATTTGTTTATCAAACTAGTGGTCAATGACTCAATGCCTTATGGGCAAAACAAACTTTTGTTTAAAATATGAATAAAACTAAGAAGAATTCGTCTTGCGATAGACTTTGTAACCATCTTTTTTGTTTGGAAAGCATGCCTGTATATGGGGTGCAATCCTCCTCCTCCCTTCATTGACCCTGCATCTTGGCATACTGAGTATGTTTTGATAAACACTAAGTATAATGGGGGCTGATAAAAGCAATGTAGTATGTGGTGATGCAAATATTGCTTACAAATATAGTTTATTGTGGTTAATTATTTGAAAGTTGTTCTGACATTTTCTTGCCTACTAATCATATATAGGTCCTCAGTAGAAAATTGTATCTCCCAGCAAAGCTTTCTCCAGATTTAAAGTAAGCATGGTAGATTATTATATGAAAATGTAGGCCTCGTTTATTAATCATCTTGTTTTTTGGTTTTTGGTTTTTGAAAAGCAAACTTGTTTTCTCTCAATTCCTGACAATGATATGCATTTTTCTTAACTGAAAGAGTTGAGTTCTTAACTAAATTCCAAAAACAAAAACAAGTTTTTCAAGACTATTTTTTTAGTTTTCAAAATTTGGCTTGGTTTTTAAAAACAATGCAAAGTAGTCCACAAAGTAAGAAATTTAGCAGTGGAAGGGGTATTTATTGACTTAATTTTAGAAAACTAAACTCTAAAAATCAGATGGTTACCAAATGGGGGTGTAACTTTTTCCTTCCCATCCTTCTCTCCATTGTCTCTTTATCTCCCTCTTTCATTGATTCTCTAGCTTCATTTGCAGGGATGGCGAACTTCCTCTTATTGTTGCTATTGATTTACAGCCGATGGCTCCAATTGAAGGTGTTATTGAAGTGCAGGGTGATATAACAAATGCTAGGACAGCTGAAGTGGTAAGCAGTGTGTATCATGTACACACTACTTTTAACATTTGATATGGTAAACGAAGACATATCCAGATCCTAATGCACCAACATGGTCAATTTTGTTTTAAAATTTCCATTTGTGATAATGTTCCTACGTTTTCTTCATACTGGATTACTTTTCTCTTTTGAATGAGTACTCACGCTGTGTTGTAAATCCCTTTTATTAAAAAAAATTGTCACATACGAAATTGTGATATCTTTTTGTTCATGGTTTATCAAATATTTAATTTTACCAGGTCATTAGGCATTTTGATGGGTGCAAGGCTGACCTGGTTGTTTGTGACGGTGCACCTGATGGTAGATATTTGTGTTTTTGAATTTTCTCTTGGGGTTCTAACTGATGATATTTTGTTACTGATTATGTGCTTGTGTTTGTAGTTACTGGTCTTCATGACATGGATGAGTTTGTTCAGTTCCAGCTCATACTAGCTGTAAGTGCAGTGTTTTTTTCTACAGACTGTTAAATTTCTCTAGATGGTGCTGATGAATAGTTTAAGATATTTTAAAAAATGCATGAGATGCATGCACATTGACAGTAATGATGTTGCTCCGGTTCTCGTTTACAGTGCGTGGAGAGAATAAAAAAATAAAACCAACAAGAAGCACTAATGAGTGTCAAATTGTTTGAATCTACAAATAGAAAATACTGTGTAACAAGTGAGCAAGGTAGATGAGATCCTTTAGGTTAATCATGGTTGCTGTATATTATGAAGTTATATCAAAATCTTCTAAAGGTTTTTCTATTGTATGTTGCCTGCACTCATTGACCTCTTTCCTATATGGATTGAATTGTGTTGCTGTCATCAGTGTCCTCTGCTGCTGCCTTGTTTTCTCTATTTCTCTCTCTTCCTTCATCTAGTTCTCAATGTAGGAAATATATGAGTTTTACAGAGATGAGCTGGACTCTTTTTTCCTTTCTTCTCAGGGTTTAACAATTGTTACGCACATACTCAGAGAAGGTGGTAAGTTCATAGCAAAGATATTCCGTGGAAAGGATACCAGCCTTTTGTATAGTCAGGTAAATGGTTGTTACAGGACCCAATAGAAATATGTAGCATACTCGTGTTGTAAAAAAATTTATGGTGTTCTCACTAAATGCATCTGCAATATGAATCAGAATCTCTCTTTCAACTTTTCTTCTTCCAATCAAGTTGGAAATTTTGCTTTGAGCTCCTTTTCCCTGTAATTATTGCATTGACGTATATGATTTTCTTAGATACGTTTAGCACTAATAAAAATTTTCAAGCATTATCATGGATTACATAAGCAACTTAATCAAGGGTGTTTGTTGTTCCATACATGGTTATGAAGCCGCCAATGATTTTTACATTTTCGAGGTTTTCTGTTGCAGCTGAAGTTATTTTTCCCTGTAGTGACTTTTGCAAAGCCAAAAAGCAGTCGGAACTCAAGTATAGGTAACCTTTCCGTTTTTCACTTTTGTTGATTGGTTGCTATAGAGGTCCATCTTTCGATTATACTTGATTTTGTTTCCAGAAAATGATACTCTCTTAATTGAAAATGGCCTCTCTCTCTCTCTCTTATTATTTTTTTAATCTCTTTATCGCATCTGATTCATAGGATTCTTGCGCATGAATTTTTAGATATTCATTGTACTTGGATTCCTTAAATCAACTTTAGATTGTTATCGATTAATACTAGCTGGTCGGACTGGTGTCCTCCCAATAACAAATTGTGGAGTTGGTACATTTAGGGGTGGTCTGTAATATAAAAAAGGTGTGAAAGAGGACAAAATAGTCAAGGGTAAAAGTAAAATTTTTGAACTTTCACTGTTGCTTTTGTGAACATCCATGAATCCTGCCATGAGTCCAAATAGTCAAATGTTTGATTACGGTAAGTCTCCTTTTATCACTTGCTTAAATGTTTTAAACAGGTAAGCAGTGATTATTTGGCATTATGTACGAGAAATAGACGTGTTAAACAACCTTATGGTAGGTAGGCCAAAATATTCAATGAGAGCATTACATTAAAAAAAAACTTTTGGTTTTTCTGCCCTTATTTAGTTGGCTTTCTCTTTTATATCACACAGTGGTATATCATACAAGATCGTTATTTGTTAACGTTTGTAAATCAAAGTTCCAAGACAACATTTAAGCTACGGTATTAACCATAAGAAATTACTAGGAAAAGAAATCAAGAGTTCAAGAACATAGTTAAAACGTCTGAAAGTTGAATGGACTAAGTGGGCACAAGTTTTAAAACTATTGAACTAAATTTGTAATTCATTCCAGTGCTTTCTTTTAGTTGCCTTGGTGAACGCACGGTAGCAACTTTTAAACAAAGTTGAGATTATGTTTTAATTTTATGTCAAAGCTCATACGTTGACTGGGTGCAAATGCAGAAGCATTTGCTGTTTGTGAGAACTATTCTCCTCCCGAAGGTTTCAATTCAAAAGATCTGCATCGCTTGCTGGAAAAGGTGGGAAGTCCGTCAGGAGGAGATGACTTAGGTAAACTCAATATCCTACCTTGATTTGGAGTTCACATTTCGTTAGACGTATCCTCAGATCAAGCATGTTCTTATCCTATTTTTAAAGATTAATTTGTTTTACAGCTGGTGCTGTGACCAAGTTGTCTACTTGTCTCTAGGCTTTTAAAAATTAGTGTCTCCTGATTTTGATGTGTGGTCAATGCCCTGGTATTTTTGGTTACATAAGAGGAAGTAATAGCATTTCTATTTTGCTACATTTTGTCCCTCTCAGATTGCAGTAGTGGGTGGCTGGAAGGTCCCAATAAAGTTTACATCCCATTTCTTGCCTGTGGGGACCTCAGTGGATATGACTCCGACCGTTCATACCCACTGCCCAGAGGTGCCGAGGGAACTTATCGGAGCTTAGATCCTGTGCAGCCTCCTATTGCCCCTCCATACAAAAGGGCTCTCGAAATGAAGAAAGCTTCCAGTCAAGGAATTCGAGAACTTGAAAAACTTTCCTTGGATTCCTGATTATCCCATTCAATGTTTTAGATAACCAACAAAGTGGTTTGGAATGGACCGGTAATTATTAGAACAGCAATCATTTCTCCCTCCTCCCCCCC

mRNA sequence

AAATACACTTAGACTTCCTTTGATGCGCAACTTTGACTTTCTTGCTGGGTTTGAATGTTGCCAGGAGGCCTGTCTGCTCCGCACTACCGGTAACTCTTGCTTGGTTCTTCAGTCATGGGCAAAGCTTCAAGAGATAAGAGGGATATATACTACCGTAAAGCAAAAGAAGAAGGTTGGCGTGCTCGAAGTGCATTTAAACTTCTTCAGATAGACGAGGAATTTAACATATTTGAAGGAGTCAAGAGAGTGGTAGATTTATGTGCAGCCCCCGGTAGCTGGAGCCAGGTCCTCAGTAGAAAATTGTATCTCCCAGCAAAGCTTTCTCCAGATTTAAAGGATGGCGAACTTCCTCTTATTGTTGCTATTGATTTACAGCCGATGGCTCCAATTGAAGGTGTTATTGAAGTGCAGGGTGATATAACAAATGCTAGGACAGCTGAAGTGGTCATTAGGCATTTTGATGGGTGCAAGGCTGACCTGGTTGTTTGTGACGGTGCACCTGATGTTACTGGTCTTCATGACATGGATGAGTTTGTTCAGTTCCAGCTCATACTAGCTGGTTTAACAATTGTTACGCACATACTCAGAGAAGGTGGTAAGTTCATAGCAAAGATATTCCGTGGAAAGGATACCAGCCTTTTGTATAGTCAGCTGAAGTTATTTTTCCCTGTAGTGACTTTTGCAAAGCCAAAAAGCAGTCGGAACTCAAGTATAGAAGCATTTGCTGTTTGTGAGAACTATTCTCCTCCCGAAGGTTTCAATTCAAAAGATCTGCATCGCTTGCTGGAAAAGGTGGGAAGTCCGTCAGGAGGAGATGACTTAGATTGCAGTAGTGGGTGGCTGGAAGGTCCCAATAAAGTTTACATCCCATTTCTTGCCTGTGGGGACCTCAGTGGATATGACTCCGACCGTTCATACCCACTGCCCAGAGGTGCCGAGGGAACTTATCGGAGCTTAGATCCTGTGCAGCCTCCTATTGCCCCTCCATACAAAAGGGCTCTCGAAATGAAGAAAGCTTCCAGTCAAGGAATTCGAGAACTTGAAAAACTTTCCTTGGATTCCTGATTATCCCATTCAATGTTTTAGATAACCAACAAAGTGGTTTGGAATGGACCGGTAATTATTAGAACAGCAATCATTTCTCCCTCCTCCCCCCC

Coding sequence (CDS)

ATGGGCAAAGCTTCAAGAGATAAGAGGGATATATACTACCGTAAAGCAAAAGAAGAAGGTTGGCGTGCTCGAAGTGCATTTAAACTTCTTCAGATAGACGAGGAATTTAACATATTTGAAGGAGTCAAGAGAGTGGTAGATTTATGTGCAGCCCCCGGTAGCTGGAGCCAGGTCCTCAGTAGAAAATTGTATCTCCCAGCAAAGCTTTCTCCAGATTTAAAGGATGGCGAACTTCCTCTTATTGTTGCTATTGATTTACAGCCGATGGCTCCAATTGAAGGTGTTATTGAAGTGCAGGGTGATATAACAAATGCTAGGACAGCTGAAGTGGTCATTAGGCATTTTGATGGGTGCAAGGCTGACCTGGTTGTTTGTGACGGTGCACCTGATGTTACTGGTCTTCATGACATGGATGAGTTTGTTCAGTTCCAGCTCATACTAGCTGGTTTAACAATTGTTACGCACATACTCAGAGAAGGTGGTAAGTTCATAGCAAAGATATTCCGTGGAAAGGATACCAGCCTTTTGTATAGTCAGCTGAAGTTATTTTTCCCTGTAGTGACTTTTGCAAAGCCAAAAAGCAGTCGGAACTCAAGTATAGAAGCATTTGCTGTTTGTGAGAACTATTCTCCTCCCGAAGGTTTCAATTCAAAAGATCTGCATCGCTTGCTGGAAAAGGTGGGAAGTCCGTCAGGAGGAGATGACTTAGATTGCAGTAGTGGGTGGCTGGAAGGTCCCAATAAAGTTTACATCCCATTTCTTGCCTGTGGGGACCTCAGTGGATATGACTCCGACCGTTCATACCCACTGCCCAGAGGTGCCGAGGGAACTTATCGGAGCTTAGATCCTGTGCAGCCTCCTATTGCCCCTCCATACAAAAGGGCTCTCGAAATGAAGAAAGCTTCCAGTCAAGGAATTCGAGAACTTGAAAAACTTTCCTTGGATTCCTGA

Protein sequence

MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLSRKLYLPAKLSPDLKDGELPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKADLVVCDGAPDVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYSQLKLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNSKDLHRLLEKVGSPSGGDDLDCSSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKKASSQGIRELEKLSLDS*

Homology

[Close]

BLAST of CSPI03G31490 vs. ExPASy Swiss-Prot
Match: Q9UET6 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Homo sapiens OX=9606 GN=FTSJ1 PE=1 SV=2)

HSP 1 Score: 354.0 bits (907), Expect = 1.8e-96
Identity = 173/300 (57.67%), Postives = 216/300 (72.00%), Query Frame = 0


Query: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS 60
           MG+ S+DKRD+YYR AKE GWRARSAFKLLQ+D+EF +F+GV R VDLCAAPGSWSQVLS
Sbjct: 1   MGRTSKDKRDVYYRLAKENGWRARSAFKLLQLDKEFQLFQGVTRAVDLCAAPGSWSQVLS 60

Query: 61  RKLYLPAKLSPDLKDGELPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKA 120
           +K+            G    +VA+DLQ MAP+ GV+++QGDIT   TA+ +I+HF GC A
Sbjct: 61  QKI-------GGQGSGH---VVAVDLQAMAPLPGVVQIQGDITQLSTAKEIIQHFKGCPA 120

Query: 121 DLVVCDGAPDVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYSQL 180
           DLVVCDGAPDVTGLHD+DE++Q QL+LA L I TH+L+ GG F+AKIFRG+D +LLYSQL
Sbjct: 121 DLVVCDGAPDVTGLHDVDEYMQAQLLLAALNIATHVLKPGGCFVAKIFRGRDVTLLYSQL 180

Query: 181 KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNSKDLHRLLEKVGSPSGGDDLDCSS 240
           ++FF  V  AKP+SSRNSSIEAFAVC+ Y PPEGF        +  +  P      D   
Sbjct: 181 QVFFSSVLCAKPRSSRNSSIEAFAVCQGYDPPEGF--------IPDLSKPLLDHSYDPDF 240

Query: 241 GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKK 300
             L+GP ++ +PF+ CGDLS YDSDRSYPL       Y+   P QPPI+PPY+ A  +K+
Sbjct: 241 NQLDGPTRIIVPFVTCGDLSSYDSDRSYPLDLEGGSEYKYTPPTQPPISPPYQEACTLKR 282

[Close]

BLAST of CSPI03G31490 vs. ExPASy Swiss-Prot
Match: O36015 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC4F10.03c PE=3 SV=1)

HSP 1 Score: 325.1 bits (832), Expect = 8.9e-88
Identity = 166/299 (55.52%), Postives = 215/299 (71.91%), Query Frame = 0


Query: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS 60
           MG++S+DKRD YYR AKE+GWRARSAFKLLQ++E+FN+FEG KRVVDLCAAPGSWSQVLS
Sbjct: 1   MGRSSKDKRDAYYRLAKEQGWRARSAFKLLQLNEQFNLFEGAKRVVDLCAAPGSWSQVLS 60

Query: 61  RKLYLPAKLSPDLKDGELPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKA 120
           R+L     +   +   E P+IVA+DLQPMAPI+GV  +Q DIT+  T  +++ HF    A
Sbjct: 61  RELL--KNIDTSIAADEKPMIVAVDLQPMAPIDGVCTLQLDITHPNTLSIILSHFGNEPA 120

Query: 121 DLVVCDGAPDVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYSQL 180
           DLVV DGAPDVTGLHD+DE++Q Q++LA   +   +L+ GGKF+AKIFRG+D SLLYSQL
Sbjct: 121 DLVVSDGAPDVTGLHDLDEYIQAQILLAAFNLAVCVLKPGGKFVAKIFRGRDVSLLYSQL 180

Query: 181 KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNSKDLHRLLEKVGSPSGGDDLDCSS 240
           +L F  V+ AKP+SSR SSIE+F VCE+++PP  F   DL + L  V  P+   ++    
Sbjct: 181 RLMFRKVSCAKPRSSRASSIESFVVCEDFNPPSNF-QPDLTKPL-CVIDPTNAHEI---- 240

Query: 241 GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMK 300
                      PF+ACGDL GYD+D +YP+    +    +LD +QPP APPYKRA+E+K
Sbjct: 241 ----------APFIACGDLDGYDADATYPVEINMKKA--TLDVIQPPTAPPYKRAIELK 279

[Close]

BLAST of CSPI03G31490 vs. ExPASy Swiss-Prot
Match: Q22031 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Caenorhabditis elegans OX=6239 GN=R74.7 PE=3 SV=3)

HSP 1 Score: 322.4 bits (825), Expect = 5.7e-87
Identity = 171/312 (54.81%), Postives = 217/312 (69.55%), Query Frame = 0


Query: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS 60
           MGK SRDKRDIYYR AKE  WRARSAFKL+QID+EF I +GV+R VDLCAAPGSWSQVLS
Sbjct: 1   MGKTSRDKRDIYYRLAKENKWRARSAFKLMQIDDEFQILKGVRRAVDLCAAPGSWSQVLS 60

Query: 61  RKLYLPAKLSPDLKDGELPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKA 120
           ++LY         ++ +   IVAIDLQPMAPI GVI++QGDIT+  TA  VI+HF G K+
Sbjct: 61  KRLY---------EEDQEAKIVAIDLQPMAPIPGVIQLQGDITSVDTANQVIKHFSGEKS 120

Query: 121 DLVVCDGAPDVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYSQL 180
           D+V+CDGAPDVTG+H +DEF+Q +LILA   I +H+L+EGG F+AKIFR +++SLLY+Q+
Sbjct: 121 DIVICDGAPDVTGIHSLDEFMQAELILAAFNITSHVLKEGGNFLAKIFRSRNSSLLYAQM 180

Query: 181 KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNSKDLHRLLEKVGSPSGGDDLDCSS 240
           K +F  V  AKP+SSR SS EAF +C +YSPPEGF        +  +G  S    LD + 
Sbjct: 181 KKYFKKVYLAKPRSSRQSSCEAFVLCLDYSPPEGF--------VPTMGKTS----LDATD 240

Query: 241 GWLEGPNKVYIPFLACGDLSGYDSDRSYPL------PRG-----AEGTYRSLDPVQPPIA 300
                P+ +   F+ CGDLSG+DS++SYPL      P+G      +  Y   D VQPP  
Sbjct: 241 ASAISPD-IIDGFVTCGDLSGWDSEKSYPLDIDACFPKGEIDEEQKKRYEFKDVVQPPTD 290

Query: 301 PPYKRALEMKKA 302
           P YK AL+ KK+
Sbjct: 301 PAYKAALDKKKS 290

[Close]

BLAST of CSPI03G31490 vs. ExPASy Swiss-Prot
Match: Q54VA8 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Dictyostelium discoideum OX=44689 GN=fsjA PE=3 SV=1)

HSP 1 Score: 302.8 bits (774), Expect = 4.7e-81
Identity = 155/274 (56.57%), Postives = 201/274 (73.36%), Query Frame = 0


Query: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS 60
           MGK+S+DKRDIYYRKAKEEGWRARSAFKL+QIDEE+ IFEGVKRVVDLCAAPGSWSQVLS
Sbjct: 1   MGKSSKDKRDIYYRKAKEEGWRARSAFKLIQIDEEYQIFEGVKRVVDLCAAPGSWSQVLS 60

Query: 61  RKLYLPAKLSPDLKDGELPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKA 120
           R++Y   K  PD+K      IVA+DLQ MAP++GV++++GDIT   T++ +I HFDG  A
Sbjct: 61  RRIYGDGK-DPDVK------IVAVDLQEMAPLKGVVQIKGDITKYETSKQIISHFDGSLA 120

Query: 121 DLVVCDGAPDVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYSQL 180
           DL++ DGAPDVTGLHD+D + Q QLIL+ L I TH L+ GG F+AK+F+G D SL+YSQ+
Sbjct: 121 DLIISDGAPDVTGLHDIDFYGQSQLILSALNITTHTLKIGGTFVAKMFKGDDMSLMYSQM 180

Query: 181 KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNSKDLHRLLE---KVGSPSGGDDLD 240
           KLFF  V+F KP SSR SS+E F +C NY PP  +N K +   LE   K+ + +  +  +
Sbjct: 181 KLFFEHVSFVKPSSSRESSLENFILCRNYQPPLNYNPKIIDPALENHNKILNNNNNNSNN 240

Query: 241 CSSGWLEGPN--------KVYIPFLACGDLS-GY 263
            ++  ++  N         + +PF+ACGDL+ GY
Sbjct: 241 NNNCNIDNENDNEFLKTDSLIVPFIACGDLNLGY 267

[Close]

BLAST of CSPI03G31490 vs. ExPASy Swiss-Prot
Match: P38238 (tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TRM7 PE=1 SV=1)

HSP 1 Score: 290.8 bits (743), Expect = 1.9e-77
Identity = 158/308 (51.30%), Postives = 211/308 (68.51%), Query Frame = 0


Query: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFE--GVKRVVDLCAAPGSWSQV 60
           MGK+S+DKRD+YYRKAKE+G+RARSAFKLLQ++++F+  +   +KRVVDLCAAPGSWSQV
Sbjct: 1   MGKSSKDKRDLYYRKAKEQGYRARSAFKLLQLNDQFHFLDDPNLKRVVDLCAAPGSWSQV 60

Query: 61  LSRKLYLPAKLSPDLKDGELPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGC 120
           LSRKL+  +  S    D E   IV++DLQPM+PI  V  +Q DIT+ +T   +++ F   
Sbjct: 61  LSRKLFDESPSS----DKEDRKIVSVDLQPMSPIPHVTTLQADITHPKTLARILKLFGNE 120

Query: 121 KADLVVCDGAPDVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYS 180
           KAD V  DGAPDVTGLHD+DE+VQ QLI++ L +   IL++GG F+AKIFRG+D  +LYS
Sbjct: 121 KADFVCSDGAPDVTGLHDLDEYVQQQLIMSALQLTACILKKGGTFVAKIFRGRDIDMLYS 180

Query: 181 QLKLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNSK-DLHRLLEKVGSPSGGDDL- 240
           QL   F  +  AKP+SSR +S+EAF VC  Y+PP  +  K D++  +++       + L 
Sbjct: 181 QLGYLFDKIVCAKPRSSRGTSLEAFIVCLGYNPPSNWTPKLDVNTSVDEFFQGCFLNKLC 240

Query: 241 --DCSSGWLEGPNKVYIPFLACGDLSGYDSDRSY-PLPRGAEGTYRSLDPVQPPIAPPYK 300
             D  S W E    +   F+ACG L  +DSD +Y  LP    GT  SLDPVQ P  PPYK
Sbjct: 241 ISDKLSHWNEEERNI-AEFMACGSLQSFDSDATYHDLPSSVAGTSSSLDPVQSPTNPPYK 300

Query: 301 RALEMKKA 302
           +ALE+K++
Sbjct: 301 KALELKRS 303

[Close]

BLAST of CSPI03G31490 vs. ExPASy TrEMBL
Match: A0A0A0LCV6 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Cucumis sativus OX=3659 GN=Csa_3G732420 PE=3 SV=1)

HSP 1 Score: 639.0 bits (1647), Expect = 1.0e-179
Identity = 316/316 (100.00%), Postives = 316/316 (100.00%), Query Frame = 0


Query: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS 60
           MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS
Sbjct: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS 60

Query: 61  RKLYLPAKLSPDLKDGELPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKA 120
           RKLYLPAKLSPDLKDGELPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKA
Sbjct: 61  RKLYLPAKLSPDLKDGELPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKA 120

Query: 121 DLVVCDGAPDVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYSQL 180
           DLVVCDGAPDVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYSQL
Sbjct: 121 DLVVCDGAPDVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYSQL 180

Query: 181 KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNSKDLHRLLEKVGSPSGGDDLDCSS 240
           KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNSKDLHRLLEKVGSPSGGDDLDCSS
Sbjct: 181 KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNSKDLHRLLEKVGSPSGGDDLDCSS 240

Query: 241 GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKK 300
           GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKK
Sbjct: 241 GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKK 300

Query: 301 ASSQGIRELEKLSLDS 317
           ASSQGIRELEKLSLDS
Sbjct: 301 ASSQGIRELEKLSLDS 316

[Close]

BLAST of CSPI03G31490 vs. ExPASy TrEMBL
Match: A0A1S3B6C8 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103486316 PE=3 SV=1)

HSP 1 Score: 636.0 bits (1639), Expect = 8.7e-179
Identity = 314/316 (99.37%), Postives = 315/316 (99.68%), Query Frame = 0


Query: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS 60
           MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS
Sbjct: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS 60

Query: 61  RKLYLPAKLSPDLKDGELPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKA 120
           RKLYLPAKLSPDLKDG+LPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKA
Sbjct: 61  RKLYLPAKLSPDLKDGDLPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKA 120

Query: 121 DLVVCDGAPDVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYSQL 180
           DLVVCDGAPDVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYSQL
Sbjct: 121 DLVVCDGAPDVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYSQL 180

Query: 181 KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNSKDLHRLLEKVGSPSGGDDLDCSS 240
           KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFN KDLHRLLEKVGSPSGGDDLDCSS
Sbjct: 181 KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSS 240

Query: 241 GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKK 300
           GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKK
Sbjct: 241 GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKK 300

Query: 301 ASSQGIRELEKLSLDS 317
           ASSQGIRELEKLSLDS
Sbjct: 301 ASSQGIRELEKLSLDS 316

[Close]

BLAST of CSPI03G31490 vs. ExPASy TrEMBL
Match: A0A6J1F1P7 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111441398 PE=3 SV=1)

HSP 1 Score: 620.9 bits (1600), Expect = 2.9e-174
Identity = 306/316 (96.84%), Postives = 310/316 (98.10%), Query Frame = 0


Query: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS 60
           MGKASRDKRDIYYRKAKEEGWRARSAFKLLQID+EFNIFEGVKRVVDLCAAPGSWSQVLS
Sbjct: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDDEFNIFEGVKRVVDLCAAPGSWSQVLS 60

Query: 61  RKLYLPAKLSPDLKDGELPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKA 120
           RKLYLPAKLSPD KDG+ PLIVAIDLQPMAPIEGVI+VQGDITNARTAEVVIRHFDGCKA
Sbjct: 61  RKLYLPAKLSPDSKDGDHPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKA 120

Query: 121 DLVVCDGAPDVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYSQL 180
           DLVVCDGAPDVTGLHDMDEFVQ QLILAGLTIVTH+LREGGKFIAKIFRGKDTSLLY QL
Sbjct: 121 DLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQL 180

Query: 181 KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNSKDLHRLLEKVGSPSGGDDLDCSS 240
           KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFN KDLHRLLEKVGSPSGGDDLDCSS
Sbjct: 181 KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSS 240

Query: 241 GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKK 300
           GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKK
Sbjct: 241 GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKK 300

Query: 301 ASSQGIRELEKLSLDS 317
           ASSQGIRELEKLS DS
Sbjct: 301 ASSQGIRELEKLSSDS 316

[Close]

BLAST of CSPI03G31490 vs. ExPASy TrEMBL
Match: A0A5A7TQN9 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G00950 PE=3 SV=1)

HSP 1 Score: 619.8 bits (1597), Expect = 6.4e-174
Identity = 305/307 (99.35%), Postives = 306/307 (99.67%), Query Frame = 0


Query: 10  DIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLSRKLYLPAKL 69
           DIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLSRKLYLPAKL
Sbjct: 70  DIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLSRKLYLPAKL 129

Query: 70  SPDLKDGELPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKADLVVCDGAP 129
           SPDLKDG+LPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKADLVVCDGAP
Sbjct: 130 SPDLKDGDLPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKADLVVCDGAP 189

Query: 130 DVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYSQLKLFFPVVTF 189
           DVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYSQLKLFFPVVTF
Sbjct: 190 DVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYSQLKLFFPVVTF 249

Query: 190 AKPKSSRNSSIEAFAVCENYSPPEGFNSKDLHRLLEKVGSPSGGDDLDCSSGWLEGPNKV 249
           AKPKSSRNSSIEAFAVCENYSPPEGFN KDLHRLLEKVGSPSGGDDLDCSSGWLEGPNKV
Sbjct: 250 AKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGPNKV 309

Query: 250 YIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKKASSQGIREL 309
           YIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKKASSQGIREL
Sbjct: 310 YIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKKASSQGIREL 369

Query: 310 EKLSLDS 317
           EKLSLDS
Sbjct: 370 EKLSLDS 376

[Close]

BLAST of CSPI03G31490 vs. ExPASy TrEMBL
Match: A0A6J1IYY9 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111481931 PE=3 SV=1)

HSP 1 Score: 614.8 bits (1584), Expect = 2.1e-172
Identity = 304/316 (96.20%), Postives = 308/316 (97.47%), Query Frame = 0


Query: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS 60
           MGKASRDKRDIYYRKAKEEGWRARSAFKLLQID+EFNI EGVKRVVDLCAAPGSWSQVLS
Sbjct: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDDEFNILEGVKRVVDLCAAPGSWSQVLS 60

Query: 61  RKLYLPAKLSPDLKDGELPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKA 120
           RKLYLPAKLSPD KDG+ PLIVAIDLQPMAPIEGVI+VQGDITNARTAEVVIRHFDGCKA
Sbjct: 61  RKLYLPAKLSPDSKDGDHPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKA 120

Query: 121 DLVVCDGAPDVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYSQL 180
           DLVVCDGAPDVTGLHDMDEFVQ QLILAGLTIVTH+LREGGKFIAKIFRGKDTSLLY QL
Sbjct: 121 DLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQL 180

Query: 181 KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNSKDLHRLLEKVGSPSGGDDLDCSS 240
           KLFFPVVTFAKPKSSRNSSIEAFAVCENYSP EGFN KDLHRLLEKVGSPSGGDDLDCSS
Sbjct: 181 KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPLEGFNPKDLHRLLEKVGSPSGGDDLDCSS 240

Query: 241 GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKK 300
           GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKK
Sbjct: 241 GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKK 300

Query: 301 ASSQGIRELEKLSLDS 317
           ASSQGIRELEKLS DS
Sbjct: 301 ASSQGIRELEKLSSDS 316

[Close]

BLAST of CSPI03G31490 vs. NCBI nr
Match: XP_004137730.1 (putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Cucumis sativus] >XP_011651872.1 putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Cucumis sativus] >XP_031738324.1 putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Cucumis sativus] >KGN58784.1 hypothetical protein Csa_000714 [Cucumis sativus])

HSP 1 Score: 639.0 bits (1647), Expect = 2.1e-179
Identity = 316/316 (100.00%), Postives = 316/316 (100.00%), Query Frame = 0


Query: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS 60
           MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS
Sbjct: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS 60

Query: 61  RKLYLPAKLSPDLKDGELPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKA 120
           RKLYLPAKLSPDLKDGELPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKA
Sbjct: 61  RKLYLPAKLSPDLKDGELPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKA 120

Query: 121 DLVVCDGAPDVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYSQL 180
           DLVVCDGAPDVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYSQL
Sbjct: 121 DLVVCDGAPDVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYSQL 180

Query: 181 KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNSKDLHRLLEKVGSPSGGDDLDCSS 240
           KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNSKDLHRLLEKVGSPSGGDDLDCSS
Sbjct: 181 KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNSKDLHRLLEKVGSPSGGDDLDCSS 240

Query: 241 GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKK 300
           GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKK
Sbjct: 241 GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKK 300

Query: 301 ASSQGIRELEKLSLDS 317
           ASSQGIRELEKLSLDS
Sbjct: 301 ASSQGIRELEKLSLDS 316

[Close]

BLAST of CSPI03G31490 vs. NCBI nr
Match: XP_008442455.1 (PREDICTED: putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase isoform X1 [Cucumis melo] >XP_008442456.1 PREDICTED: putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase isoform X1 [Cucumis melo] >XP_008442457.1 PREDICTED: putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase isoform X1 [Cucumis melo])

HSP 1 Score: 636.0 bits (1639), Expect = 1.8e-178
Identity = 314/316 (99.37%), Postives = 315/316 (99.68%), Query Frame = 0


Query: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS 60
           MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS
Sbjct: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS 60

Query: 61  RKLYLPAKLSPDLKDGELPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKA 120
           RKLYLPAKLSPDLKDG+LPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKA
Sbjct: 61  RKLYLPAKLSPDLKDGDLPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKA 120

Query: 121 DLVVCDGAPDVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYSQL 180
           DLVVCDGAPDVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYSQL
Sbjct: 121 DLVVCDGAPDVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYSQL 180

Query: 181 KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNSKDLHRLLEKVGSPSGGDDLDCSS 240
           KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFN KDLHRLLEKVGSPSGGDDLDCSS
Sbjct: 181 KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSS 240

Query: 241 GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKK 300
           GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKK
Sbjct: 241 GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKK 300

Query: 301 ASSQGIRELEKLSLDS 317
           ASSQGIRELEKLSLDS
Sbjct: 301 ASSQGIRELEKLSLDS 316

[Close]

BLAST of CSPI03G31490 vs. NCBI nr
Match: XP_038903231.1 (putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Benincasa hispida])

HSP 1 Score: 621.7 bits (1602), Expect = 3.5e-174
Identity = 306/316 (96.84%), Postives = 311/316 (98.42%), Query Frame = 0


Query: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS 60
           MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGV+RVVDLCAAPGSWSQVLS
Sbjct: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVERVVDLCAAPGSWSQVLS 60

Query: 61  RKLYLPAKLSPDLKDGELPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKA 120
           RKL+LPAKLSPD KDG+LPLIVAIDLQPMAPIEGVI+VQGDITNARTAEVVIRHFDGCKA
Sbjct: 61  RKLHLPAKLSPDSKDGDLPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKA 120

Query: 121 DLVVCDGAPDVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYSQL 180
           DLVVCDGAPDVTGLHDMDEFVQ QLILAGLTIVTH+LREGGKFIAKIFRGKDTSLLY QL
Sbjct: 121 DLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQL 180

Query: 181 KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNSKDLHRLLEKVGSPSGGDDLDCSS 240
           KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFN KDLHRLLEKVGSPSGGDDLDCSS
Sbjct: 181 KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSS 240

Query: 241 GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKK 300
           GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYR LDPVQPPIAPPYKRALEMKK
Sbjct: 241 GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRCLDPVQPPIAPPYKRALEMKK 300

Query: 301 ASSQGIRELEKLSLDS 317
           ASSQGIRELEKLSLDS
Sbjct: 301 ASSQGIRELEKLSLDS 316

[Close]

BLAST of CSPI03G31490 vs. NCBI nr
Match: KAG6580486.1 (putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 620.9 bits (1600), Expect = 6.0e-174
Identity = 306/316 (96.84%), Postives = 310/316 (98.10%), Query Frame = 0


Query: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS 60
           MGKASRDKRDIYYRKAKEEGWRARSAFKLLQID+EFNIFEGVKRVVDLCAAPGSWSQVLS
Sbjct: 21  MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDDEFNIFEGVKRVVDLCAAPGSWSQVLS 80

Query: 61  RKLYLPAKLSPDLKDGELPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKA 120
           RKLYLPAKLSPD KDG+ PLIVAIDLQPMAPIEGVI+VQGDITNARTAEVVIRHFDGCKA
Sbjct: 81  RKLYLPAKLSPDSKDGDHPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKA 140

Query: 121 DLVVCDGAPDVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYSQL 180
           DLVVCDGAPDVTGLHDMDEFVQ QLILAGLTIVTH+LREGGKFIAKIFRGKDTSLLY QL
Sbjct: 141 DLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQL 200

Query: 181 KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNSKDLHRLLEKVGSPSGGDDLDCSS 240
           KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFN KDLHRLLEKVGSPSGGDDLDCSS
Sbjct: 201 KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSS 260

Query: 241 GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKK 300
           GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKK
Sbjct: 261 GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKK 320

Query: 301 ASSQGIRELEKLSLDS 317
           ASSQGIRELEKLS DS
Sbjct: 321 ASSQGIRELEKLSSDS 336

[Close]

BLAST of CSPI03G31490 vs. NCBI nr
Match: XP_022934133.1 (putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Cucurbita moschata] >XP_022934134.1 putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Cucurbita moschata] >XP_023528668.1 putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Cucurbita pepo subsp. pepo] >XP_023528669.1 putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Cucurbita pepo subsp. pepo] >KAG7017237.1 putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 620.9 bits (1600), Expect = 6.0e-174
Identity = 306/316 (96.84%), Postives = 310/316 (98.10%), Query Frame = 0


Query: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS 60
           MGKASRDKRDIYYRKAKEEGWRARSAFKLLQID+EFNIFEGVKRVVDLCAAPGSWSQVLS
Sbjct: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDDEFNIFEGVKRVVDLCAAPGSWSQVLS 60

Query: 61  RKLYLPAKLSPDLKDGELPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKA 120
           RKLYLPAKLSPD KDG+ PLIVAIDLQPMAPIEGVI+VQGDITNARTAEVVIRHFDGCKA
Sbjct: 61  RKLYLPAKLSPDSKDGDHPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKA 120

Query: 121 DLVVCDGAPDVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYSQL 180
           DLVVCDGAPDVTGLHDMDEFVQ QLILAGLTIVTH+LREGGKFIAKIFRGKDTSLLY QL
Sbjct: 121 DLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQL 180

Query: 181 KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNSKDLHRLLEKVGSPSGGDDLDCSS 240
           KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFN KDLHRLLEKVGSPSGGDDLDCSS
Sbjct: 181 KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSS 240

Query: 241 GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKK 300
           GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKK
Sbjct: 241 GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKK 300

Query: 301 ASSQGIRELEKLSLDS 317
           ASSQGIRELEKLS DS
Sbjct: 301 ASSQGIRELEKLSSDS 316

[Close]

BLAST of CSPI03G31490 vs. TAIR 10
Match: AT5G01230.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 575.5 bits (1482), Expect = 2.7e-164
Identity = 282/305 (92.46%), Postives = 295/305 (96.72%), Query Frame = 0


Query: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS 60
           MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS
Sbjct: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS 60

Query: 61  RKLYLPAKLSPDLKDGELPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKA 120
           R+LYLPAK S + KDG+LPLIVAIDLQPMAPIEGVI+VQGDITNARTAEVVIRHFDGCKA
Sbjct: 61  RQLYLPAKSSAESKDGDLPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKA 120

Query: 121 DLVVCDGAPDVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYSQL 180
           DLVVCDGAPDVTGLHDMDEFVQ QLILAGLTIVTHIL+EGGKFIAKIFRGKDTSLLY QL
Sbjct: 121 DLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHILKEGGKFIAKIFRGKDTSLLYCQL 180

Query: 181 KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNSKDLHRLLEKVGSPSGGDDLDCSS 240
           KLFFP VTFAKPKSSRNSSIEAFAVCENYSPPEGFN +DLHRLLEKVGSPSGG DLDCSS
Sbjct: 181 KLFFPTVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPRDLHRLLEKVGSPSGGSDLDCSS 240

Query: 241 GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEG-TYRSLDPVQPPIAPPYKRALEMK 300
           GWLEGPNKVYIPFLACGDL+GYDSDRSYPLPR A+G +Y+SLDP+QPPIAPPYKRALE+K
Sbjct: 241 GWLEGPNKVYIPFLACGDLTGYDSDRSYPLPREADGSSYQSLDPIQPPIAPPYKRALELK 300

Query: 301 KASSQ 305
           KAS+Q
Sbjct: 301 KASAQ 305

[Close]

BLAST of CSPI03G31490 vs. TAIR 10
Match: AT4G25730.1 (FtsJ-like methyltransferase family protein )

HSP 1 Score: 122.5 bits (306), Expect = 6.2e-28
Identity = 79/240 (32.92%), Postives = 129/240 (53.75%), Query Frame = 0


Query: 1   MGKA-SRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVL 60
           MGK   + + D YYR AKE G+R+R+++KLLQ+D ++++      V+DLCAAPG W QV 
Sbjct: 1   MGKVKGKHRLDKYYRLAKERGFRSRASYKLLQLDAKYSLLHSAHAVLDLCAAPGGWMQVA 60

Query: 61  SRKLYLPAKLSPDLKDGELPLIVAIDLQPMAPIEGVIEVQGDIT----NARTAEVVIRHF 120
             K+ + +            L++ IDL P+ P+ G + +  DIT     ++  +V+ +H 
Sbjct: 61  VEKVPVGS------------LVLGIDLVPILPVRGCVTMTQDITRTECKSKIKQVMEQH- 120

Query: 121 DGCKA-DLVVCDGAPDVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKD-T 180
            G  A +LV+ DG+P+V G    +   Q  L++  + + T  L   G  + K+FR +D  
Sbjct: 121 -GVSAFNLVLHDGSPNVGGAWAQEAMSQNALVIDSVRLATEFLARNGNLVTKVFRSRDYN 180

Query: 181 SLLYSQLKLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNSK--DLHRLLEKVGSPS 232
           S+LY   +LF  V  F KP +SR++S E + V   Y  P   + +  D   L ++   P+
Sbjct: 181 SVLYCLGRLFEKVEVF-KPPASRSASAETYLVGLKYLAPAKIDPRLLDYRHLFKESAEPT 225

[Close]

BLAST of CSPI03G31490 vs. TAIR 10
Match: AT5G01230.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 120.2 bits (300), Expect = 3.1e-27
Identity = 57/57 (100.00%), Postives = 57/57 (100.00%), Query Frame = 0


Query: 1  MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQ 58
          MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQ
Sbjct: 1  MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQ 57

[Close]

BLAST of CSPI03G31490 vs. TAIR 10
Match: AT5G13830.1 (FtsJ-like methyltransferase family protein )

HSP 1 Score: 71.2 bits (173), Expect = 1.6e-12
Identity = 54/225 (24.00%), Postives = 98/225 (43.56%), Query Frame = 0


Query: 10  DIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLSRKLYLPAKL 69
           D +YR+A+  G+ ARSAFKLLQI +++ + +    V+DL  APG+W QV  + L      
Sbjct: 8   DFFYREAQRLGYVARSAFKLLQIQKQYKLIKPGSSVLDLGCAPGAWLQVACQSL------ 67

Query: 70  SPDLKDGELPLIVAIDLQ----PMAPIEGVIEVQGDITNARTAEVVIRHFDGCKADLVVC 129
              L+ G   ++V +D++    P      V  +  D+ N    ++           +++ 
Sbjct: 68  -GPLRSG--GIVVGMDIKKVKVPPQCDSRVQTIAADVLNFPRQKIRELSPQQLGFSVILS 127

Query: 130 DGAPDVTGLHDMDEFVQFQLILAGLTIVT---------------------HILREGGKFI 189
           D    V+G+   D  +  +L +  L +                        +LR GG  +
Sbjct: 128 DMCHSVSGITTRDAALSAELGMRALDLAVGQAAISQSPNDDDGGPNESRPGVLRHGGHLV 187

Query: 190 AKIFRGKDTSLLYSQLKLFFPVVTFAKPKSSRNSSIEAFAVCENY 210
            K+   +D        K  F   ++ +PK++R SS E + +C+ +
Sbjct: 188 IKLLESEDAQDFARICKPIFNKASWLRPKATRPSSREIYLICQGF 223

The following BLAST results are available for this feature:

BLAST of CSPI03G31490 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9UET6	1.8e-96	57.67	Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Homo sapien...	[more]
O36015	8.9e-88	55.52	Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Schizosacch...	[more]
Q22031	5.7e-87	54.81	Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Caenorhabdi...	[more]
Q54VA8	4.7e-81	56.57	Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Dictyosteli...	[more]
P38238	1.9e-77	51.30	tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Saccharomyces cerevi...	[more]

BLAST of CSPI03G31490 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LCV6	1.0e-179	100.00	Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Cucumis sat...	[more]
A0A1S3B6C8	8.7e-179	99.37	Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Cucumis mel...	[more]
A0A6J1F1P7	2.9e-174	96.84	Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Cucurbita m...	[more]
A0A5A7TQN9	6.4e-174	99.35	Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Cucumis mel...	[more]
A0A6J1IYY9	2.1e-172	96.20	Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Cucurbita m...	[more]

BLAST of CSPI03G31490 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004137730.1	2.1e-179	100.00	putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Cucumis sativ...	[more]
XP_008442455.1	1.8e-178	99.37	PREDICTED: putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase iso...	[more]
XP_038903231.1	3.5e-174	96.84	putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Benincasa his...	[more]
KAG6580486.1	6.0e-174	96.84	putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase, partial [Cucu...	[more]
XP_022934133.1	6.0e-174	96.84	putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Cucurbita mos...	[more]

BLAST of CSPI03G31490 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G01230.1	2.7e-164	92.46	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT4G25730.1	6.2e-28	32.92	FtsJ-like methyltransferase family protein	[more]
AT5G01230.2	3.1e-27	100.00	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT5G13830.1	1.6e-12	24.00	FtsJ-like methyltransferase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 1..127 e-value: 7.6E-43 score: 148.5
None	No IPR available	PANTHER	PTHR10920	RIBOSOMAL RNA METHYLTRANSFERASE	coord: 1..220
None	No IPR available	PANTHER	PTHR10920:SF25	TRNA (CYTIDINE(32)/GUANOSINE(34)-2'-O)-METHYLTRANSFERASE-RELATED	coord: 1..220
IPR002877	Ribosomal RNA methyltransferase FtsJ domain	PFAM	PF01728	FtsJ	coord: 1..121 e-value: 2.1E-35 score: 122.4
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 7..121

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G31490.1	CSPI03G31490.1	mRNA
CSPI03G31490.2	CSPI03G31490.2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0002181	cytoplasmic translation
biological_process	GO:0002128	tRNA nucleoside ribose methylation
biological_process	GO:0032259	methylation
biological_process	GO:0001510	RNA methylation
biological_process	GO:0008033	tRNA processing
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0008175	tRNA methyltransferase activity
molecular_function	GO:0008168	methyltransferase activity