CSPI03G31330 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI03G31330
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	RING-type domain-containing protein
Location	Chr3: 28386011 .. 28389295 (+)
RNA-Seq Expression	CSPI03G31330
Synteny	CSPI03G31330

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CGAGAGAAAAGCCAAGTGAGTCCCACTCCCTTTCTCTACGGCTGTTTTTCACTTCTCGAATTGCTAAATTCGCTTCGAAAAACCCTTTTTGCCGAAGAACTGCGAATAGAAAGGGGATTTCATAATTTCATGTTTTGGATTTCTTGTAGAGGTGAACCGTTGAAGTCGAACGAGGTGGAATGAATTCTCGCTTGTAGATTACGGAGGTATTTTTTATTTCGTTGTTTTTGAGTTGGTTTTTTGTGTTCTTGATGGTTTCTTCTGAATTGAATGGAAATTTTTCTATTGAATTGATTTAGATGAGGTAGGAGTTGCTGCTGCTGATACTGGAAGTGGTGGATGGGAGTTTTTTGGTGTGGAAATTAACTCAAAATGCGAAAATCTTTCAAGGACTCCCTTAAGGCTCTCGAGGCTGATATTCAGTTCGCCAATACCTTGTAATTTCTCCATATGCTGTTCAACTTTCTTATAATTTCATATCTACTCTGGTCTCTGGACTGTGTTTGAATTGCTATTGCAATGTCGGCGTTTCCATTGTCTGGAAATTATACGCTATGTTCGTTAAATTTTCATGATTCGAACTGAATCGAGTTTCAATATTTTGTTTCTTGTGATATATGCTTTCTCCGTGCTTAGATTAGAATGGTATTCGGGCTGGTTTCGCTGCGCGTGGAGTTTGAAGTTCAGGGAAGTTTCTATGAAAGTGGTTTGATGGGTTATGATTTCTGAGAGAGCCGCGAGTTTTAGGTTTTGTATCACATCATCTGTACATTCTTGAACACTTAGTAGATTAGATATTTAGTCATCTCGATCTTATTTCTGGAATTGTTCTCCGCCTGCTCATATATGTTATTTTCTGCTTAAGATTTATGAAAACGGTTTATTTAAGATTTGTTATCAACTTCTGTGTTTCTTATTTTCTAGATGTTAAGTGTGTTTGAATGTATATTTTCTTCCTTTTGTGTGGGTTATCTTGTATGCTTAGGTGGTTTGTGAACTGTCACATTCTCCAGCTAGTAGTGTTGTAAGATATTTCGGCTTCTCAGGATTGTATAACTACAGAATTTTACTGCAACAAATCATTTCTCTTGTTTTTAGTGTGCAATAGTTCCGGCGAGCCTTGTTTAATTTATAAGCATTGAAATATAATGCTTTAATACTGAACAGGGCTTCTGATTATCCGAAGGAATACGATGGAGCATGCCTGCAAATGAGATTATCCTATAGCCCGGCTGCGCAGTTTTTTCTCTTCTTCGTGCAATGGACTGATTGTCATCTTGCTGGTGCATTGGGCCTGCTCAGAATTCTAATATATAAGGTACTTCCAGACTGCATGAAGAATGACATTTTGACTCCTACATTGCCCAAGTGTGGTGATTGAAGTGGATCAAACTGATCACGAAAGAAAAAAAATTTGATGAAGTAGCTTATACTACTTGCCCCTACACATAATCTTGTCAATCCCATGAATGTGCTAAATTTGTTTTTAGGCATATGAAGACGGGAAGACTACCATGTCTATTCAAGAAAGAAAGGCTAGTCTCAAAGAGTTCTATGGTAAGTGGCTAATAAGATAAAGACTGCCCTCTCGTCAGCTTGAGGTGTATTAGCATTTGGAGTCTTGCTAATTCTATTTTTTAGTTCTGCAAATACAGGGGTGATATTTCCTTCGCTAGTGCAACTTCAAAAAGGAATTACTGACATAGAAGAAAGAAAACAGAGAGAAGTTTATGCTGCAAAATTCAAAAGAACAGATCGGTTGAACAAAGGAAAGATTTCTGAAATCGACTTGGAAAGAGAGGAAGAATGCGGTATTTGCATGGAGCTAAACAACAAGGTTGTCTTGCCTAATTGCAATCATTCAATGTGCATGAAGTGCTACAGGAGTTGGTAAGCTCTCTGCTGTTTTCATGTGCTATGGTTACTAACTAAGGGCTACTTTGTTCTTAGCAAAAAAGTGAACTGGATTATTTGCAGCTGATTTTTTGCAACTTATTATCTCTTTTTCAGGCGCACTCGATCGCAATCATGCCCCTTCTGCCGTGACAGTCTAAAAAGAGTCAACTCTGGAGATCTTTGGATATGTACCAGTAGTAGTGAAATTGTCGACTTATCCTTCATCTCTAGGGACAATCTGAAGAGGCTTTTCATGTTCATTAATAAGTTGCCTCTCATTGTTCCTGATCCAAAACTCATCTCTTATTATCTGAATCATTATTGAACACTCCAGGCTTGTCCCGGAAGTAATCTGTAAATAAAGACCCATTACCCTTGTTGTGCATAATTCTTATCAGTATAACGTGCACATCTAAAATTTCCACATGCACCCCTGTTGTGAAAGTCTGCTAAATTTTTTGTTATCTGAAGCCTGCTCCAAGTCTTCCATCATTACTATTTAGGCCGTTTTAACTCAACTTTATTCCTGGGCTCTTGTTATCCCGAAAACAGATTGAGGCTGTGGAATCACGCTTGACAAAACGATGAAGATGATGGCATGCGGTACTCAATCACCAGGTTCTTACTTTTTTCACATATTGTATTAATTTTCACCAACCCTTGACATAGATTTCCAAAATTTGTCATTTTTACTAATTTTGGCTTTAGCTAAAACTTTGAATCATATATCATTAAATGGCAGGAGTATTTCGAGCAACCACGTGTAGGAGAAATGCCAAATGGGGTTTACCCGAGTATAAAAAAGAAAATGGAAGTCGAGGGTGTCACGAGCATTTGTCCTCATCAAATCCAATTTTTCATACTAAAATTAGGGGTTTTCAAATGATTCGACGAACTATAGTGATTGAGAATTTAGAGTTTGATTTTCTATTCTCACTTTCCTTCTTAGATCTGATACATTCATTTCAGATGCTCCGCTGCAAGAAACTGTGCAGCAGACTATCGTGAGTTCTCGTTGGTTTCTTGGGATGATTCTTGCGTCATCGACTTTGCTTTTGTGTATGGGCATTCAATTTTATTTCCTCTTCTAACTAGGAAGATAGGATGCCAACAACTAACCATTTTATAAGACGACAAGATAAAAGTATGGTACAGGATCATCTAAAAATAGAACGGAAGGGTAGTTGTGTTGCAACTTATAGAGTTGCTCAATAACAGAGTATGGGATTTGCGTAAAAGGAAATAGGTATCTTGTGTAGCTCTTATGGAATATAGATTTTGTTGTTCTTATGCTTGTGATTATTCTCGTCACATGTGGGTATCGCAGCATGATACTATTTCGTGCTATTGTTGTAGCATGGAATACTCTTTAGAACTGTTAACACTCCC

mRNA sequence

CGAGAGAAAAGCCAAGTGAGTCCCACTCCCTTTCTCTACGGCTGTTTTTCACTTCTCGAATTGCTAAATTCGCTTCGAAAAACCCTTTTTGCCGAAGAACTGCGAATAGAAAGGGGATTTCATAATTTCATGTTTTGGATTTCTTGTAGAGGTGAACCGTTGAAGTCGAACGAGGTGGAATGAATTCTCGCTTGTAGATTACGGAGATGAGGTAGGAGTTGCTGCTGCTGATACTGGAAGTGGTGGATGGGAGTTTTTTGGTGTGGAAATTAACTCAAAATGCGAAAATCTTTCAAGGACTCCCTTAAGGCTCTCGAGGCTGATATTCAGTTCGCCAATACCTTGGCTTCTGATTATCCGAAGGAATACGATGGAGCATGCCTGCAAATGAGATTATCCTATAGCCCGGCTGCGCAGTTTTTTCTCTTCTTCGTGCAATGGACTGATTGTCATCTTGCTGGTGCATTGGGCCTGCTCAGAATTCTAATATATAAGGCATATGAAGACGGGAAGACTACCATGTCTATTCAAGAAAGAAAGGCTAGTCTCAAAGAGTTCTATGGGGTGATATTTCCTTCGCTAGTGCAACTTCAAAAAGGAATTACTGACATAGAAGAAAGAAAACAGAGAGAAGTTTATGCTGCAAAATTCAAAAGAACAGATCGGTTGAACAAAGGAAAGATTTCTGAAATCGACTTGGAAAGAGAGGAAGAATGCGGTATTTGCATGGAGCTAAACAACAAGGTTGTCTTGCCTAATTGCAATCATTCAATGTGCATGAAGTGCTACAGGAGTTGGCGCACTCGATCGCAATCATGCCCCTTCTGCCGTGACAGTCTAAAAAGAGTCAACTCTGGAGATCTTTGGATATGTACCAGTAGTAGTGAAATTGTCGACTTATCCTTCATCTCTAGGGACAATCTGAAGAGGCTTTTCATGTTCATTAATAAGTTGCCTCTCATTGTTCCTGATCCAAAACTCATCTCTTATTATCTGAATCATTATTGAACACTCCAGGCTTGTCCCGGAAGTAATCTGTAAATAAAGACCCATTACCCTTGTTGTGCATAATTCTTATCAGTATAACGTGCACATCTAAAATTTCCACATGCACCCCTGTTGTGAAAGTCTGCTAAATTTTTTGTTATCTGAAGCCTGCTCCAAGTCTTCCATCATTACTATTTAGGCCGTTTTAACTCAACTTTATTCCTGGGCTCTTGTTATCCCGAAAACAGATTGAGGCTGTGGAATCACGCTTGACAAAACGATGAAGATGATGGCATGCGGTACTCAATCACCAGGAGTATTTCGAGCAACCACGTGTAGGAGAAATGCCAAATGGGGTTTACCCGAGTATAAAAAAGAAAATGGAAGTCGAGGGTGTCACGAGCATTTGTCCTCATCAAATCCAATTTTTCATACTAAAATTAGGGGTTTTCAAATGATTCGACGAACTATAGTGATTGAGAATTTAGAGTTTGATTTTCTATTCTCACTTTCCTTCTTAGATCTGATACATTCATTTCAGATGCTCCGCTGCAAGAAACTGTGCAGCAGACTATCGTGAGTTCTCGTTGGTTTCTTGGGATGATTCTTGCGTCATCGACTTTGCTTTTGTGTATGGGCATTCAATTTTATTTCCTCTTCTAACTAGGAAGATAGGATGCCAACAACTAACCATTTTATAAGACGACAAGATAAAAGTATGGTACAGGATCATCTAAAAATAGAACGGAAGGGTAGTTGTGTTGCAACTTATAGAGTTGCTCAATAACAGAGTATGGGATTTGCGTAAAAGGAAATAGGTATCTTGTGTAGCTCTTATGGAATATAGATTTTGTTGTTCTTATGCTTGTGATTATTCTCGTCACATGTGGGTATCGCAGCATGATACTATTTCGTGCTATTGTTGTAGCATGGAATACTCTTTAGAACTGTTAACACTCCC

Coding sequence (CDS)

ATGCGAAAATCTTTCAAGGACTCCCTTAAGGCTCTCGAGGCTGATATTCAGTTCGCCAATACCTTGGCTTCTGATTATCCGAAGGAATACGATGGAGCATGCCTGCAAATGAGATTATCCTATAGCCCGGCTGCGCAGTTTTTTCTCTTCTTCGTGCAATGGACTGATTGTCATCTTGCTGGTGCATTGGGCCTGCTCAGAATTCTAATATATAAGGCATATGAAGACGGGAAGACTACCATGTCTATTCAAGAAAGAAAGGCTAGTCTCAAAGAGTTCTATGGGGTGATATTTCCTTCGCTAGTGCAACTTCAAAAAGGAATTACTGACATAGAAGAAAGAAAACAGAGAGAAGTTTATGCTGCAAAATTCAAAAGAACAGATCGGTTGAACAAAGGAAAGATTTCTGAAATCGACTTGGAAAGAGAGGAAGAATGCGGTATTTGCATGGAGCTAAACAACAAGGTTGTCTTGCCTAATTGCAATCATTCAATGTGCATGAAGTGCTACAGGAGTTGGCGCACTCGATCGCAATCATGCCCCTTCTGCCGTGACAGTCTAAAAAGAGTCAACTCTGGAGATCTTTGGATATGTACCAGTAGTAGTGAAATTGTCGACTTATCCTTCATCTCTAGGGACAATCTGAAGAGGCTTTTCATGTTCATTAATAAGTTGCCTCTCATTGTTCCTGATCCAAAACTCATCTCTTATTATCTGAATCATTATTGA

Protein sequence

MRKSFKDSLKALEADIQFANTLASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDGKTTMSIQERKASLKEFYGVIFPSLVQLQKGITDIEERKQREVYAAKFKRTDRLNKGKISEIDLEREEECGICMELNNKVVLPNCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWICTSSSEIVDLSFISRDNLKRLFMFINKLPLIVPDPKLISYYLNHY*

Homology

[Close]

BLAST of CSPI03G31330 vs. ExPASy Swiss-Prot
Match: Q9M022 (E3 ubiquitin-protein ligase AIRP2 OS=Arabidopsis thaliana OX=3702 GN=AIRP2 PE=1 SV=1)

HSP 1 Score: 372.1 bits (954), Expect = 4.8e-102
Identity = 174/237 (73.42%), Postives = 204/237 (86.08%), Query Frame = 0


Query: 1   MRKSFKDSLKALEADIQFANTLASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLA 60
           MRKSFKDSLKALEADIQFANTLAS+YP+EYDG  +QMRLSYSPAA  FLF +QWTDCH A
Sbjct: 1   MRKSFKDSLKALEADIQFANTLASEYPEEYDGGYVQMRLSYSPAAHLFLFLLQWTDCHFA 60

Query: 61  GALGLLRILIYKAYEDGKTTMSIQERKASLKEFYGVIFPSLVQLQKGITDIEERKQREVY 120
           GALGLLRILIYKAY DGKTTMS+ ERK S++EFY V+FPSL+QL  GITD+EERKQ+E+ 
Sbjct: 61  GALGLLRILIYKAYVDGKTTMSLHERKTSIREFYDVLFPSLLQLHGGITDVEERKQKEIC 120

Query: 121 AAKFKRTDRLNKGKISEIDLEREEECGICMELNNKVVLPNCNHSMCMKCYRSWRTRSQSC 180
             ++++ DR +KGK+SEIDLEREEECGIC+E+ NKVVLP CNHSMC+ CYR+WR RSQSC
Sbjct: 121 DKRYRKKDRTDKGKMSEIDLEREEECGICLEIRNKVVLPTCNHSMCINCYRNWRARSQSC 180

Query: 181 PFCRDSLKRVNSGDLWICTSSSEIVDLSFISRDNLKRLFMFINKLPLIVPDPKLISY 238
           PFCR SLKRVNSGDLWI T S+EI DL  I ++NLKRL ++I+KLPL+  DP L+ Y
Sbjct: 181 PFCRGSLKRVNSGDLWIYTCSAEIADLPAIYKENLKRLLIYIDKLPLVTSDPNLVPY 237

[Close]

BLAST of CSPI03G31330 vs. ExPASy Swiss-Prot
Match: Q803C1 (E3 ubiquitin-protein ligase rnf8 OS=Danio rerio OX=7955 GN=rnf8 PE=2 SV=1)

HSP 1 Score: 52.4 bits (124), Expect = 8.5e-06
Identity = 34/105 (32.38%), Postives = 51/105 (48.57%), Query Frame = 0


Query: 84  QERKASLKEFYGVIFPSLVQLQKGITDIEERKQREVYAAK-FKRTDRLNKGKI----SEI 143
           ++ + +LKE   VI   L    +G  ++ + K +E+   K  K   +  K ++    +E+
Sbjct: 327 KQLEEALKEHRKVI-EELKHAWQGFKEVLQAKDKELEVTKEEKEKAKAQKEEVVTQMTEV 386

Query: 144 DLEREEECGICMELNNKVVLPNCNHSMCMKCYRSWRTRSQSCPFC 184
            LE E +C IC EL  + V  NC HS C  C   WR R   CP C
Sbjct: 387 -LESELQCSICSELFIEAVTLNCAHSFCQHCISEWRNRKDKCPMC 429

[Close]

BLAST of CSPI03G31330 vs. ExPASy Swiss-Prot
Match: Q13064 (Probable E3 ubiquitin-protein ligase makorin-3 OS=Homo sapiens OX=9606 GN=MKRN3 PE=1 SV=1)

HSP 1 Score: 50.8 bits (120), Expect = 2.5e-05
Identity = 26/71 (36.62%), Postives = 39/71 (54.93%), Query Frame = 0


Query: 143 EEECGICMEL-------NNK--VVLPNCNHSMCMKCYRSWRTRSQ-------SCPFCRDS 198
           ++ CGICME+       N++   +L NCNHS C++C R WR+  Q       SCP CR +
Sbjct: 308 DKVCGICMEVVYEKANPNDRRFGILSNCNHSFCIRCIRRWRSARQFENRIVKSCPQCRVT 367

[Close]

BLAST of CSPI03G31330 vs. ExPASy Swiss-Prot
Match: Q13434 (Putative E3 ubiquitin-protein ligase makorin-4 OS=Homo sapiens OX=9606 GN=MKRN4P PE=5 SV=1)

HSP 1 Score: 50.8 bits (120), Expect = 2.5e-05
Identity = 24/76 (31.58%), Postives = 38/76 (50.00%), Query Frame = 0


Query: 138 IDLEREEECGICMEL---------NNKVVLPNCNHSMCMKCYRSWRTRSQ-------SCP 197
           +   +++ CGICME+         +   +L NCNH+ C+KC R WR+  +       SCP
Sbjct: 308 VQRSKDKVCGICMEVVYEKANPNEHRFGILSNCNHTFCLKCIRKWRSAKEFESRIVKSCP 367

[Close]

BLAST of CSPI03G31330 vs. ExPASy Swiss-Prot
Match: Q9UHC7 (E3 ubiquitin-protein ligase makorin-1 OS=Homo sapiens OX=9606 GN=MKRN1 PE=1 SV=3)

HSP 1 Score: 50.1 bits (118), Expect = 4.2e-05
Identity = 25/76 (32.89%), Postives = 36/76 (47.37%), Query Frame = 0


Query: 138 IDLEREEECGICMEL---------NNKVVLPNCNHSMCMKCYRSWRTRSQ-------SCP 197
           +   ++  CGICME+             +L NCNH+ C+KC R WR+  Q       SCP
Sbjct: 273 VQRSKDMVCGICMEVVYEKANPSERRFGILSNCNHTYCLKCIRKWRSAKQFESKIIKSCP 332

[Close]

BLAST of CSPI03G31330 vs. ExPASy TrEMBL
Match: A0A0A0LA78 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G731740 PE=4 SV=1)

HSP 1 Score: 493.8 bits (1270), Expect = 4.1e-136
Identity = 242/242 (100.00%), Postives = 242/242 (100.00%), Query Frame = 0


Query: 1   MRKSFKDSLKALEADIQFANTLASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLA 60
           MRKSFKDSLKALEADIQFANTLASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLA
Sbjct: 1   MRKSFKDSLKALEADIQFANTLASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLA 60

Query: 61  GALGLLRILIYKAYEDGKTTMSIQERKASLKEFYGVIFPSLVQLQKGITDIEERKQREVY 120
           GALGLLRILIYKAYEDGKTTMSIQERKASLKEFYGVIFPSLVQLQKGITDIEERKQREVY
Sbjct: 61  GALGLLRILIYKAYEDGKTTMSIQERKASLKEFYGVIFPSLVQLQKGITDIEERKQREVY 120

Query: 121 AAKFKRTDRLNKGKISEIDLEREEECGICMELNNKVVLPNCNHSMCMKCYRSWRTRSQSC 180
           AAKFKRTDRLNKGKISEIDLEREEECGICMELNNKVVLPNCNHSMCMKCYRSWRTRSQSC
Sbjct: 121 AAKFKRTDRLNKGKISEIDLEREEECGICMELNNKVVLPNCNHSMCMKCYRSWRTRSQSC 180

Query: 181 PFCRDSLKRVNSGDLWICTSSSEIVDLSFISRDNLKRLFMFINKLPLIVPDPKLISYYLN 240
           PFCRDSLKRVNSGDLWICTSSSEIVDLSFISRDNLKRLFMFINKLPLIVPDPKLISYYLN
Sbjct: 181 PFCRDSLKRVNSGDLWICTSSSEIVDLSFISRDNLKRLFMFINKLPLIVPDPKLISYYLN 240

Query: 241 HY 243
           HY
Sbjct: 241 HY 242

[Close]

BLAST of CSPI03G31330 vs. ExPASy TrEMBL
Match: A0A5D3DMZ7 (E3 ubiquitin-protein ligase RNF8-B OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G00850 PE=4 SV=1)

HSP 1 Score: 483.0 bits (1242), Expect = 7.2e-133
Identity = 235/242 (97.11%), Postives = 240/242 (99.17%), Query Frame = 0


Query: 1   MRKSFKDSLKALEADIQFANTLASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLA 60
           MRKSFKDSLKALEADIQFANTLASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLA
Sbjct: 1   MRKSFKDSLKALEADIQFANTLASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLA 60

Query: 61  GALGLLRILIYKAYEDGKTTMSIQERKASLKEFYGVIFPSLVQLQKGITDIEERKQREVY 120
           GALGLLRILIYKAYEDGKTTMSIQERKASLKEFYGVIFPSLVQLQ+GITDIEERKQREVY
Sbjct: 61  GALGLLRILIYKAYEDGKTTMSIQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVY 120

Query: 121 AAKFKRTDRLNKGKISEIDLEREEECGICMELNNKVVLPNCNHSMCMKCYRSWRTRSQSC 180
           AAK++RTD LNKGKISEID+EREEECGICMELN KVVLPNCNHSMCMKCYRSWRTRSQSC
Sbjct: 121 AAKYRRTDPLNKGKISEIDMEREEECGICMELNTKVVLPNCNHSMCMKCYRSWRTRSQSC 180

Query: 181 PFCRDSLKRVNSGDLWICTSSSEIVDLSFISRDNLKRLFMFINKLPLIVPDPKLISYYLN 240
           PFCRDSLKRVNSGDLWICTSSSEIVDLSFISR+NLKRLFMFINKLPLIVPDPKLISYYLN
Sbjct: 181 PFCRDSLKRVNSGDLWICTSSSEIVDLSFISRENLKRLFMFINKLPLIVPDPKLISYYLN 240

Query: 241 HY 243
           HY
Sbjct: 241 HY 242

[Close]

BLAST of CSPI03G31330 vs. ExPASy TrEMBL
Match: A0A1S3B5N9 (uncharacterized protein LOC103486301 OS=Cucumis melo OX=3656 GN=LOC103486301 PE=4 SV=1)

HSP 1 Score: 483.0 bits (1242), Expect = 7.2e-133
Identity = 235/242 (97.11%), Postives = 240/242 (99.17%), Query Frame = 0


Query: 1   MRKSFKDSLKALEADIQFANTLASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLA 60
           MRKSFKDSLKALEADIQFANTLASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLA
Sbjct: 1   MRKSFKDSLKALEADIQFANTLASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLA 60

Query: 61  GALGLLRILIYKAYEDGKTTMSIQERKASLKEFYGVIFPSLVQLQKGITDIEERKQREVY 120
           GALGLLRILIYKAYEDGKTTMSIQERKASLKEFYGVIFPSLVQLQ+GITDIEERKQREVY
Sbjct: 61  GALGLLRILIYKAYEDGKTTMSIQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVY 120

Query: 121 AAKFKRTDRLNKGKISEIDLEREEECGICMELNNKVVLPNCNHSMCMKCYRSWRTRSQSC 180
           AAK++RTD LNKGKISEID+EREEECGICMELN KVVLPNCNHSMCMKCYRSWRTRSQSC
Sbjct: 121 AAKYRRTDPLNKGKISEIDMEREEECGICMELNTKVVLPNCNHSMCMKCYRSWRTRSQSC 180

Query: 181 PFCRDSLKRVNSGDLWICTSSSEIVDLSFISRDNLKRLFMFINKLPLIVPDPKLISYYLN 240
           PFCRDSLKRVNSGDLWICTSSSEIVDLSFISR+NLKRLFMFINKLPLIVPDPKLISYYLN
Sbjct: 181 PFCRDSLKRVNSGDLWICTSSSEIVDLSFISRENLKRLFMFINKLPLIVPDPKLISYYLN 240

Query: 241 HY 243
           HY
Sbjct: 241 HY 242

[Close]

BLAST of CSPI03G31330 vs. ExPASy TrEMBL
Match: A0A6J1CVC0 (uncharacterized protein LOC111015127 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111015127 PE=4 SV=1)

HSP 1 Score: 459.5 bits (1181), Expect = 8.5e-126
Identity = 224/242 (92.56%), Postives = 235/242 (97.11%), Query Frame = 0


Query: 1   MRKSFKDSLKALEADIQFANTLASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLA 60
           MRKSFKDSLKALEADIQFANTLASDYP+EYDGACLQMRLSYSPAAQFFLFFVQWTDCHLA
Sbjct: 1   MRKSFKDSLKALEADIQFANTLASDYPREYDGACLQMRLSYSPAAQFFLFFVQWTDCHLA 60

Query: 61  GALGLLRILIYKAYEDGKTTMSIQERKASLKEFYGVIFPSLVQLQKGITDIEERKQREVY 120
           GALGLLRILIYKAYEDGKTTMSIQERKASLKEFYGVIFPSL+QLQ GI DIE+RKQREVY
Sbjct: 61  GALGLLRILIYKAYEDGKTTMSIQERKASLKEFYGVIFPSLLQLQGGINDIEDRKQREVY 120

Query: 121 AAKFKRTDRLNKGKISEIDLEREEECGICMELNNKVVLPNCNHSMCMKCYRSWRTRSQSC 180
           AAK+KR D+L++GKI+EIDLEREEECGICMELN  VVLP+CNHSMCMKCYRSWRTRSQSC
Sbjct: 121 AAKYKRKDQLDRGKITEIDLEREEECGICMELNCMVVLPSCNHSMCMKCYRSWRTRSQSC 180

Query: 181 PFCRDSLKRVNSGDLWICTSSSEIVDLSFISRDNLKRLFMFINKLPLIVPDPKLISYYLN 240
           PFCRDSLKRVNSGDLWI T SSEIVDLSFISR+NLKRLFMFI+KLPLIVPDPKLISYYLN
Sbjct: 181 PFCRDSLKRVNSGDLWIYTGSSEIVDLSFISRENLKRLFMFIDKLPLIVPDPKLISYYLN 240

Query: 241 HY 243
           HY
Sbjct: 241 HY 242

[Close]

BLAST of CSPI03G31330 vs. ExPASy TrEMBL
Match: A0A6J1F2V6 (LON peptidase N-terminal domain and RING finger protein 1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111441644 PE=4 SV=1)

HSP 1 Score: 450.7 bits (1158), Expect = 4.0e-123
Identity = 219/242 (90.50%), Postives = 230/242 (95.04%), Query Frame = 0


Query: 1   MRKSFKDSLKALEADIQFANTLASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLA 60
           MRKSFKDSLKALEADIQFANTLASDYPKEY GACLQMRLSYSPAAQFFLFFVQWTDCHLA
Sbjct: 1   MRKSFKDSLKALEADIQFANTLASDYPKEYGGACLQMRLSYSPAAQFFLFFVQWTDCHLA 60

Query: 61  GALGLLRILIYKAYEDGKTTMSIQERKASLKEFYGVIFPSLVQLQKGITDIEERKQREVY 120
           GALGLLRILIYKAYEDGKTTMSIQERKASLKEFYGVIFPSLV LQ+G  DIE+R+ RE Y
Sbjct: 61  GALGLLRILIYKAYEDGKTTMSIQERKASLKEFYGVIFPSLVLLQRGTNDIEDRQHREAY 120

Query: 121 AAKFKRTDRLNKGKISEIDLEREEECGICMELNNKVVLPNCNHSMCMKCYRSWRTRSQSC 180
           AAK+KR D+L+KGKISEIDLEREEECGICMELN+KVVLP C HSMCMKCYRSWR RSQSC
Sbjct: 121 AAKYKRKDQLDKGKISEIDLEREEECGICMELNSKVVLPTCCHSMCMKCYRSWRARSQSC 180

Query: 181 PFCRDSLKRVNSGDLWICTSSSEIVDLSFISRDNLKRLFMFINKLPLIVPDPKLISYYLN 240
           PFCRDSL+RVNSGDLWICTSS+EIVDL FIS +NLKRLFMFI+KLPLIVPDPKLISYYLN
Sbjct: 181 PFCRDSLRRVNSGDLWICTSSTEIVDLPFISSENLKRLFMFIDKLPLIVPDPKLISYYLN 240

Query: 241 HY 243
           HY
Sbjct: 241 HY 242

[Close]

BLAST of CSPI03G31330 vs. NCBI nr
Match: XP_011651863.1 (E3 ubiquitin-protein ligase AIRP2 [Cucumis sativus] >XP_031738614.1 E3 ubiquitin-protein ligase AIRP2 [Cucumis sativus] >KGN58763.1 hypothetical protein Csa_000946 [Cucumis sativus])

HSP 1 Score: 493.8 bits (1270), Expect = 8.4e-136
Identity = 242/242 (100.00%), Postives = 242/242 (100.00%), Query Frame = 0


Query: 1   MRKSFKDSLKALEADIQFANTLASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLA 60
           MRKSFKDSLKALEADIQFANTLASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLA
Sbjct: 1   MRKSFKDSLKALEADIQFANTLASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLA 60

Query: 61  GALGLLRILIYKAYEDGKTTMSIQERKASLKEFYGVIFPSLVQLQKGITDIEERKQREVY 120
           GALGLLRILIYKAYEDGKTTMSIQERKASLKEFYGVIFPSLVQLQKGITDIEERKQREVY
Sbjct: 61  GALGLLRILIYKAYEDGKTTMSIQERKASLKEFYGVIFPSLVQLQKGITDIEERKQREVY 120

Query: 121 AAKFKRTDRLNKGKISEIDLEREEECGICMELNNKVVLPNCNHSMCMKCYRSWRTRSQSC 180
           AAKFKRTDRLNKGKISEIDLEREEECGICMELNNKVVLPNCNHSMCMKCYRSWRTRSQSC
Sbjct: 121 AAKFKRTDRLNKGKISEIDLEREEECGICMELNNKVVLPNCNHSMCMKCYRSWRTRSQSC 180

Query: 181 PFCRDSLKRVNSGDLWICTSSSEIVDLSFISRDNLKRLFMFINKLPLIVPDPKLISYYLN 240
           PFCRDSLKRVNSGDLWICTSSSEIVDLSFISRDNLKRLFMFINKLPLIVPDPKLISYYLN
Sbjct: 181 PFCRDSLKRVNSGDLWICTSSSEIVDLSFISRDNLKRLFMFINKLPLIVPDPKLISYYLN 240

Query: 241 HY 243
           HY
Sbjct: 241 HY 242

[Close]

BLAST of CSPI03G31330 vs. NCBI nr
Match: XP_008442427.1 (PREDICTED: uncharacterized protein LOC103486301 [Cucumis melo] >KAA0044165.1 E3 ubiquitin-protein ligase RNF8-B [Cucumis melo var. makuwa] >TYK24968.1 E3 ubiquitin-protein ligase RNF8-B [Cucumis melo var. makuwa])

HSP 1 Score: 483.0 bits (1242), Expect = 1.5e-132
Identity = 235/242 (97.11%), Postives = 240/242 (99.17%), Query Frame = 0


Query: 1   MRKSFKDSLKALEADIQFANTLASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLA 60
           MRKSFKDSLKALEADIQFANTLASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLA
Sbjct: 1   MRKSFKDSLKALEADIQFANTLASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLA 60

Query: 61  GALGLLRILIYKAYEDGKTTMSIQERKASLKEFYGVIFPSLVQLQKGITDIEERKQREVY 120
           GALGLLRILIYKAYEDGKTTMSIQERKASLKEFYGVIFPSLVQLQ+GITDIEERKQREVY
Sbjct: 61  GALGLLRILIYKAYEDGKTTMSIQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVY 120

Query: 121 AAKFKRTDRLNKGKISEIDLEREEECGICMELNNKVVLPNCNHSMCMKCYRSWRTRSQSC 180
           AAK++RTD LNKGKISEID+EREEECGICMELN KVVLPNCNHSMCMKCYRSWRTRSQSC
Sbjct: 121 AAKYRRTDPLNKGKISEIDMEREEECGICMELNTKVVLPNCNHSMCMKCYRSWRTRSQSC 180

Query: 181 PFCRDSLKRVNSGDLWICTSSSEIVDLSFISRDNLKRLFMFINKLPLIVPDPKLISYYLN 240
           PFCRDSLKRVNSGDLWICTSSSEIVDLSFISR+NLKRLFMFINKLPLIVPDPKLISYYLN
Sbjct: 181 PFCRDSLKRVNSGDLWICTSSSEIVDLSFISRENLKRLFMFINKLPLIVPDPKLISYYLN 240

Query: 241 HY 243
           HY
Sbjct: 241 HY 242

[Close]

BLAST of CSPI03G31330 vs. NCBI nr
Match: XP_038904042.1 (E3 ubiquitin-protein ligase AIRP2 isoform X1 [Benincasa hispida])

HSP 1 Score: 474.9 bits (1221), Expect = 4.0e-130
Identity = 232/242 (95.87%), Postives = 238/242 (98.35%), Query Frame = 0


Query: 1   MRKSFKDSLKALEADIQFANTLASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLA 60
           MRKSFKDSLKALEADIQFANTLASDYPKEY GACLQMRLSYSPAAQFFLFFVQWTDCH A
Sbjct: 1   MRKSFKDSLKALEADIQFANTLASDYPKEYGGACLQMRLSYSPAAQFFLFFVQWTDCHFA 60

Query: 61  GALGLLRILIYKAYEDGKTTMSIQERKASLKEFYGVIFPSLVQLQKGITDIEERKQREVY 120
           GALGLLRILIYKAYEDGKTTMSIQERKASLKEFYGVIFPSLVQLQ+GITDIEERKQREVY
Sbjct: 61  GALGLLRILIYKAYEDGKTTMSIQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVY 120

Query: 121 AAKFKRTDRLNKGKISEIDLEREEECGICMELNNKVVLPNCNHSMCMKCYRSWRTRSQSC 180
           AAKFKR DRL+KGKISEIDLEREEECGICMELN+KVVLP+CNHSMCMKCYRSWR RSQSC
Sbjct: 121 AAKFKRKDRLDKGKISEIDLEREEECGICMELNSKVVLPSCNHSMCMKCYRSWRARSQSC 180

Query: 181 PFCRDSLKRVNSGDLWICTSSSEIVDLSFISRDNLKRLFMFINKLPLIVPDPKLISYYLN 240
           PFCRDSLKRVNSGDLWICTSSSEIVDLSFISR+NLKRLFMFI+KLPLIVPDPKLISYYLN
Sbjct: 181 PFCRDSLKRVNSGDLWICTSSSEIVDLSFISRENLKRLFMFIDKLPLIVPDPKLISYYLN 240

Query: 241 HY 243
           HY
Sbjct: 241 HY 242

[Close]

BLAST of CSPI03G31330 vs. NCBI nr
Match: XP_022145744.1 (uncharacterized protein LOC111015127 isoform X1 [Momordica charantia] >XP_022145745.1 uncharacterized protein LOC111015127 isoform X1 [Momordica charantia] >XP_022145746.1 uncharacterized protein LOC111015127 isoform X1 [Momordica charantia])

HSP 1 Score: 459.5 bits (1181), Expect = 1.8e-125
Identity = 224/242 (92.56%), Postives = 235/242 (97.11%), Query Frame = 0


Query: 1   MRKSFKDSLKALEADIQFANTLASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLA 60
           MRKSFKDSLKALEADIQFANTLASDYP+EYDGACLQMRLSYSPAAQFFLFFVQWTDCHLA
Sbjct: 1   MRKSFKDSLKALEADIQFANTLASDYPREYDGACLQMRLSYSPAAQFFLFFVQWTDCHLA 60

Query: 61  GALGLLRILIYKAYEDGKTTMSIQERKASLKEFYGVIFPSLVQLQKGITDIEERKQREVY 120
           GALGLLRILIYKAYEDGKTTMSIQERKASLKEFYGVIFPSL+QLQ GI DIE+RKQREVY
Sbjct: 61  GALGLLRILIYKAYEDGKTTMSIQERKASLKEFYGVIFPSLLQLQGGINDIEDRKQREVY 120

Query: 121 AAKFKRTDRLNKGKISEIDLEREEECGICMELNNKVVLPNCNHSMCMKCYRSWRTRSQSC 180
           AAK+KR D+L++GKI+EIDLEREEECGICMELN  VVLP+CNHSMCMKCYRSWRTRSQSC
Sbjct: 121 AAKYKRKDQLDRGKITEIDLEREEECGICMELNCMVVLPSCNHSMCMKCYRSWRTRSQSC 180

Query: 181 PFCRDSLKRVNSGDLWICTSSSEIVDLSFISRDNLKRLFMFINKLPLIVPDPKLISYYLN 240
           PFCRDSLKRVNSGDLWI T SSEIVDLSFISR+NLKRLFMFI+KLPLIVPDPKLISYYLN
Sbjct: 181 PFCRDSLKRVNSGDLWIYTGSSEIVDLSFISRENLKRLFMFIDKLPLIVPDPKLISYYLN 240

Query: 241 HY 243
           HY
Sbjct: 241 HY 242

[Close]

BLAST of CSPI03G31330 vs. NCBI nr
Match: XP_023527445.1 (LON peptidase N-terminal domain and RING finger protein 1-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 451.8 bits (1161), Expect = 3.7e-123
Identity = 220/242 (90.91%), Postives = 230/242 (95.04%), Query Frame = 0


Query: 1   MRKSFKDSLKALEADIQFANTLASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLA 60
           MRKSFKDSLKALEADIQFANTLASDYPKEY GACLQMRLSYSPAAQFFLFFVQWTDCHLA
Sbjct: 1   MRKSFKDSLKALEADIQFANTLASDYPKEYGGACLQMRLSYSPAAQFFLFFVQWTDCHLA 60

Query: 61  GALGLLRILIYKAYEDGKTTMSIQERKASLKEFYGVIFPSLVQLQKGITDIEERKQREVY 120
           GALGLLRILIYKAYEDGKTTMSIQERKASLKEFYGVIFPSLV LQ+G  DIE+R+ RE Y
Sbjct: 61  GALGLLRILIYKAYEDGKTTMSIQERKASLKEFYGVIFPSLVLLQRGTNDIEDRQHREAY 120

Query: 121 AAKFKRTDRLNKGKISEIDLEREEECGICMELNNKVVLPNCNHSMCMKCYRSWRTRSQSC 180
           AAK+KR D+L+KGKISEIDLEREEECGICMELN+KVVLP C HSMCMKCYRSWR RSQSC
Sbjct: 121 AAKYKRKDQLDKGKISEIDLEREEECGICMELNSKVVLPTCCHSMCMKCYRSWRARSQSC 180

Query: 181 PFCRDSLKRVNSGDLWICTSSSEIVDLSFISRDNLKRLFMFINKLPLIVPDPKLISYYLN 240
           PFCRDSL+RVNSGDLWICTSSSEIVDL FIS +NLKRLFMFI+KLPLIVPDPKLISYYLN
Sbjct: 181 PFCRDSLRRVNSGDLWICTSSSEIVDLPFISSENLKRLFMFIDKLPLIVPDPKLISYYLN 240

Query: 241 HY 243
           HY
Sbjct: 241 HY 242

[Close]

BLAST of CSPI03G31330 vs. TAIR 10
Match: AT5G01520.1 (RING/U-box superfamily protein )

HSP 1 Score: 372.1 bits (954), Expect = 3.4e-103
Identity = 174/237 (73.42%), Postives = 204/237 (86.08%), Query Frame = 0


Query: 1   MRKSFKDSLKALEADIQFANTLASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLA 60
           MRKSFKDSLKALEADIQFANTLAS+YP+EYDG  +QMRLSYSPAA  FLF +QWTDCH A
Sbjct: 1   MRKSFKDSLKALEADIQFANTLASEYPEEYDGGYVQMRLSYSPAAHLFLFLLQWTDCHFA 60

Query: 61  GALGLLRILIYKAYEDGKTTMSIQERKASLKEFYGVIFPSLVQLQKGITDIEERKQREVY 120
           GALGLLRILIYKAY DGKTTMS+ ERK S++EFY V+FPSL+QL  GITD+EERKQ+E+ 
Sbjct: 61  GALGLLRILIYKAYVDGKTTMSLHERKTSIREFYDVLFPSLLQLHGGITDVEERKQKEIC 120

Query: 121 AAKFKRTDRLNKGKISEIDLEREEECGICMELNNKVVLPNCNHSMCMKCYRSWRTRSQSC 180
             ++++ DR +KGK+SEIDLEREEECGIC+E+ NKVVLP CNHSMC+ CYR+WR RSQSC
Sbjct: 121 DKRYRKKDRTDKGKMSEIDLEREEECGICLEIRNKVVLPTCNHSMCINCYRNWRARSQSC 180

Query: 181 PFCRDSLKRVNSGDLWICTSSSEIVDLSFISRDNLKRLFMFINKLPLIVPDPKLISY 238
           PFCR SLKRVNSGDLWI T S+EI DL  I ++NLKRL ++I+KLPL+  DP L+ Y
Sbjct: 181 PFCRGSLKRVNSGDLWIYTCSAEIADLPAIYKENLKRLLIYIDKLPLVTSDPNLVPY 237

[Close]

BLAST of CSPI03G31330 vs. TAIR 10
Match: AT3G47160.1 (RING/U-box superfamily protein )

HSP 1 Score: 343.6 bits (880), Expect = 1.3e-94
Identity = 159/228 (69.74%), Postives = 191/228 (83.77%), Query Frame = 0


Query: 4   SFKDSLKALEADIQFANTLASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGAL 63
           SFKDSLKALEADIQ ANT+A DYP+E DGA +QMRLSY+PAAQF LF VQWTDCHLAG L
Sbjct: 5   SFKDSLKALEADIQHANTVALDYPREKDGARVQMRLSYTPAAQFLLFLVQWTDCHLAGTL 64

Query: 64  GLLRILIYKAYEDGKTTMSIQERKASLKEFYGVIFPSLVQLQKGITDIEERKQREVYAAK 123
           GLLR+LIY  Y DGKTTMS+ ERK S+K+FY VIFPSL+QL++GITD+++RKQ+EV   +
Sbjct: 65  GLLRVLIYMTYADGKTTMSVYERKTSIKDFYAVIFPSLLQLERGITDLDDRKQKEVCKIR 124

Query: 124 FKRTDRLNKGKISEIDLEREEECGICMELNNKVVLPNCNHSMCMKCYRSWRTRSQSCPFC 183
           ++  D   K K+SEID+EREEECGICME+NN VVLPNC HS+C+KCYR W  RS+SCPFC
Sbjct: 125 YRNKDETEKVKLSEIDIEREEECGICMEMNNMVVLPNCTHSLCIKCYRDWHGRSESCPFC 184

Query: 184 RDSLKRVNSGDLWICTSSSEIVDLSFISRDNLKRLFMFINKLPLIVPD 232
           RDSLKRVNSGDLW+    S+ V++  I R+N KRLF++I KLPL+VPD
Sbjct: 185 RDSLKRVNSGDLWMLMEKSDTVNMYTIERENKKRLFVYIEKLPLVVPD 232

[Close]

BLAST of CSPI03G31330 vs. TAIR 10
Match: AT3G47160.2 (RING/U-box superfamily protein )

HSP 1 Score: 335.1 bits (858), Expect = 4.7e-92
Identity = 159/240 (66.25%), Postives = 191/240 (79.58%), Query Frame = 0


Query: 4   SFKDSLKALEADIQFANTLAS------------DYPKEYDGACLQMRLSYSPAAQFFLFF 63
           SFKDSLKALEADIQ ANT+ S            DYP+E DGA +QMRLSY+PAAQF LF 
Sbjct: 5   SFKDSLKALEADIQHANTVLSNELGDEVIFRALDYPREKDGARVQMRLSYTPAAQFLLFL 64

Query: 64  VQWTDCHLAGALGLLRILIYKAYEDGKTTMSIQERKASLKEFYGVIFPSLVQLQKGITDI 123
           VQWTDCHLAG LGLLR+LIY  Y DGKTTMS+ ERK S+K+FY VIFPSL+QL++GITD+
Sbjct: 65  VQWTDCHLAGTLGLLRVLIYMTYADGKTTMSVYERKTSIKDFYAVIFPSLLQLERGITDL 124

Query: 124 EERKQREVYAAKFKRTDRLNKGKISEIDLEREEECGICMELNNKVVLPNCNHSMCMKCYR 183
           ++RKQ+EV   +++  D   K K+SEID+EREEECGICME+NN VVLPNC HS+C+KCYR
Sbjct: 125 DDRKQKEVCKIRYRNKDETEKVKLSEIDIEREEECGICMEMNNMVVLPNCTHSLCIKCYR 184

Query: 184 SWRTRSQSCPFCRDSLKRVNSGDLWICTSSSEIVDLSFISRDNLKRLFMFINKLPLIVPD 232
            W  RS+SCPFCRDSLKRVNSGDLW+    S+ V++  I R+N KRLF++I KLPL+VPD
Sbjct: 185 DWHGRSESCPFCRDSLKRVNSGDLWMLMEKSDTVNMYTIERENKKRLFVYIEKLPLVVPD 244

[Close]

BLAST of CSPI03G31330 vs. TAIR 10
Match: AT5G58787.1 (RING/U-box superfamily protein )

HSP 1 Score: 332.4 bits (851), Expect = 3.0e-91
Identity = 158/228 (69.30%), Postives = 192/228 (84.21%), Query Frame = 0


Query: 4   SFKDSLKALEADIQFANTLASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGAL 63
           SFKDSLKALEADIQ ANTLA DYP+E DGA +QMRLSYSP AQFFLF VQWTDC LAG L
Sbjct: 5   SFKDSLKALEADIQHANTLALDYPREKDGARVQMRLSYSPTAQFFLFLVQWTDCKLAGFL 64

Query: 64  GLLRILIYKAYEDGKTTMSIQERKASLKEFYGVIFPSLVQLQKGITDIEERKQREVYAAK 123
           GLLR+LIY  Y DGKTTMS+ ERKAS++EF  VI PSL QLQ+G+TDI++ KQ+EV   +
Sbjct: 65  GLLRVLIYMTYADGKTTMSVYERKASIREFQAVILPSLSQLQRGVTDIDDSKQKEVCKMR 124

Query: 124 FKRTDRLNKGKISEIDLEREEECGICMELNNKVVLPNCNHSMCMKCYRSWRTRSQSCPFC 183
           +++ D   + ++SEI++EREEECGICME+N+KVVLPNC HS+C+KCYR WR RSQSCPFC
Sbjct: 125 YRKKD---ESEMSEIEIEREEECGICMEMNSKVVLPNCTHSLCIKCYRDWRGRSQSCPFC 184

Query: 184 RDSLKRVNSGDLWICTSSSEIVDLSFISRDNLKRLFMFINKLPLIVPD 232
           RDSLKRV+SGDLW+    ++ V+L+ I+R+N KRLFM+I KLPL+VPD
Sbjct: 185 RDSLKRVDSGDLWMFLDQNDTVNLTAIARENQKRLFMYIEKLPLVVPD 229

[Close]

BLAST of CSPI03G31330 vs. TAIR 10
Match: AT5G58787.2 (RING/U-box superfamily protein )

HSP 1 Score: 302.4 bits (773), Expect = 3.3e-82
Identity = 148/228 (64.91%), Postives = 180/228 (78.95%), Query Frame = 0


Query: 4   SFKDSLKALEADIQFANTLASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGAL 63
           SFKDSLKALEADIQ ANTLA DYP+E DGA +QMRLSYSP AQFFLF VQWTDC LAG L
Sbjct: 5   SFKDSLKALEADIQHANTLALDYPREKDGARVQMRLSYSPTAQFFLFLVQWTDCKLAGFL 64

Query: 64  GLLRILIYKAYEDGKTTMSIQERKASLKEFYGVIFPSLVQLQKGITDIEERKQREVYAAK 123
           GLLR+LIY  Y DGKTTMS+ ERKAS++EF                DI++ KQ+EV   +
Sbjct: 65  GLLRVLIYMTYADGKTTMSVYERKASIREF---------------QDIDDSKQKEVCKMR 124

Query: 124 FKRTDRLNKGKISEIDLEREEECGICMELNNKVVLPNCNHSMCMKCYRSWRTRSQSCPFC 183
           +++ D   + ++SEI++EREEECGICME+N+KVVLPNC HS+C+KCYR WR RSQSCPFC
Sbjct: 125 YRKKD---ESEMSEIEIEREEECGICMEMNSKVVLPNCTHSLCIKCYRDWRGRSQSCPFC 184

Query: 184 RDSLKRVNSGDLWICTSSSEIVDLSFISRDNLKRLFMFINKLPLIVPD 232
           RDSLKRV+SGDLW+    ++ V+L+ I+R+N KRLFM+I KLPL+VPD
Sbjct: 185 RDSLKRVDSGDLWMFLDQNDTVNLTAIARENQKRLFMYIEKLPLVVPD 214

The following BLAST results are available for this feature:

BLAST of CSPI03G31330 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M022	4.8e-102	73.42	E3 ubiquitin-protein ligase AIRP2 OS=Arabidopsis thaliana OX=3702 GN=AIRP2 PE=1 ...	[more]
Q803C1	8.5e-06	32.38	E3 ubiquitin-protein ligase rnf8 OS=Danio rerio OX=7955 GN=rnf8 PE=2 SV=1	[more]
Q13064	2.5e-05	36.62	Probable E3 ubiquitin-protein ligase makorin-3 OS=Homo sapiens OX=9606 GN=MKRN3 ...	[more]
Q13434	2.5e-05	31.58	Putative E3 ubiquitin-protein ligase makorin-4 OS=Homo sapiens OX=9606 GN=MKRN4P...	[more]
Q9UHC7	4.2e-05	32.89	E3 ubiquitin-protein ligase makorin-1 OS=Homo sapiens OX=9606 GN=MKRN1 PE=1 SV=3	[more]

BLAST of CSPI03G31330 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LA78	4.1e-136	100.00	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G731740 P...	[more]
A0A5D3DMZ7	7.2e-133	97.11	E3 ubiquitin-protein ligase RNF8-B OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A1S3B5N9	7.2e-133	97.11	uncharacterized protein LOC103486301 OS=Cucumis melo OX=3656 GN=LOC103486301 PE=...	[more]
A0A6J1CVC0	8.5e-126	92.56	uncharacterized protein LOC111015127 isoform X1 OS=Momordica charantia OX=3673 G...	[more]
A0A6J1F2V6	4.0e-123	90.50	LON peptidase N-terminal domain and RING finger protein 1-like isoform X2 OS=Cuc...	[more]

BLAST of CSPI03G31330 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011651863.1	8.4e-136	100.00	E3 ubiquitin-protein ligase AIRP2 [Cucumis sativus] >XP_031738614.1 E3 ubiquitin...	[more]
XP_008442427.1	1.5e-132	97.11	PREDICTED: uncharacterized protein LOC103486301 [Cucumis melo] >KAA0044165.1 E3 ...	[more]
XP_038904042.1	4.0e-130	95.87	E3 ubiquitin-protein ligase AIRP2 isoform X1 [Benincasa hispida]	[more]
XP_022145744.1	1.8e-125	92.56	uncharacterized protein LOC111015127 isoform X1 [Momordica charantia] >XP_022145...	[more]
XP_023527445.1	3.7e-123	90.91	LON peptidase N-terminal domain and RING finger protein 1-like isoform X2 [Cucur...	[more]

BLAST of CSPI03G31330 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G01520.1	3.4e-103	73.42	RING/U-box superfamily protein	[more]
AT3G47160.1	1.3e-94	69.74	RING/U-box superfamily protein	[more]
AT3G47160.2	4.7e-92	66.25	RING/U-box superfamily protein	[more]
AT5G58787.1	3.0e-91	69.30	RING/U-box superfamily protein	[more]
AT5G58787.2	3.3e-82	64.91	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 134..171 e-value: 2.0E-6 score: 37.3
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 134..172 score: 12.179465
None	No IPR available	PFAM	PF13920	zf-C3HC4_3	coord: 131..177 e-value: 1.5E-9 score: 37.6
None	No IPR available	PANTHER	PTHR15315:SF31	E3 UBIQUITIN-PROTEIN LIGASE AIRP2	coord: 8..227
None	No IPR available	PANTHER	PTHR15315	RING FINGER PROTEIN 41, 151	coord: 8..227
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 116..177
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 108..180 e-value: 2.9E-15 score: 57.4
IPR017907	Zinc finger, RING-type, conserved site	PROSITE	PS00518	ZF_RING_1	coord: 149..158

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G31330.1	CSPI03G31330.1	mRNA
CSPI03G31330.2	CSPI03G31330.2	mRNA
CSPI03G31330.3	CSPI03G31330.3	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009789	positive regulation of abscisic acid-activated signaling pathway
biological_process	GO:0016567	protein ubiquitination
biological_process	GO:0009651	response to salt stress
biological_process	GO:0006511	ubiquitin-dependent protein catabolic process
cellular_component	GO:0005829	cytosol
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0061630	ubiquitin protein ligase activity