CSPI03G29910 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI03G29910
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	nuclear envelope integral membrane protein 1 isoform X1
Location	Chr3: 27396396 .. 27396866 (-)
RNA-Seq Expression	CSPI03G29910
Synteny	CSPI03G29910

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTAGGAGTCAACCATATAATTTTCCCCCGCCATTTCACTTCCGTCCATTCACCGGTGATTCTTGCAGATCTGAGAAGAACAAGAATGCCGGTGAAGGTAGTGGACGCCATCCTCTCCGACGTCAATGCTGACAAACTCCAAAACAACAAACCTACTTTCATCCTCTTATTGGCTGCCACATCTCTCAGCTTGTTGGCTTCTGTTTGTCTGAAAGGAACATGGTTGGACATTTGGGTAGACCACAAGTTTATCCATTATGAAGATGGAATAGTTGATAAAAGTATAACGCTTTTTATCACATGGTTCATTAGAATTTTGGCTACTTTTCTTATTCTTCATTGTTCTTTGGATCCGTTGTTGGCAATAGGAGTTCTGATATGTGGAATGATGGCTTCATCACAGTTGATAAAAATATTGAAGGCTCATGTCAAATTTATTCAAATCTTTCAAGGAAACCTTCAAGACTAA

mRNA sequence

ATGTTAGGAGTCAACCATATAATTTTCCCCCGCCATTTCACTTCCGTCCATTCACCGGTGATTCTTGCAGATCTGAGAAGAACAAGAATGCCGGTGAAGGTAGTGGACGCCATCCTCTCCGACGTCAATGCTGACAAACTCCAAAACAACAAACCTACTTTCATCCTCTTATTGGCTGCCACATCTCTCAGCTTGTTGGCTTCTGTTTGTCTGAAAGGAACATGGTTGGACATTTGGGTAGACCACAAGTTTATCCATTATGAAGATGGAATAGTTGATAAAAGTATAACGCTTTTTATCACATGGTTCATTAGAATTTTGGCTACTTTTCTTATTCTTCATTGTTCTTTGGATCCGTTGTTGGCAATAGGAGTTCTGATATGTGGAATGATGGCTTCATCACAGTTGATAAAAATATTGAAGGCTCATGTCAAATTTATTCAAATCTTTCAAGGAAACCTTCAAGACTAA

Coding sequence (CDS)

ATGTTAGGAGTCAACCATATAATTTTCCCCCGCCATTTCACTTCCGTCCATTCACCGGTGATTCTTGCAGATCTGAGAAGAACAAGAATGCCGGTGAAGGTAGTGGACGCCATCCTCTCCGACGTCAATGCTGACAAACTCCAAAACAACAAACCTACTTTCATCCTCTTATTGGCTGCCACATCTCTCAGCTTGTTGGCTTCTGTTTGTCTGAAAGGAACATGGTTGGACATTTGGGTAGACCACAAGTTTATCCATTATGAAGATGGAATAGTTGATAAAAGTATAACGCTTTTTATCACATGGTTCATTAGAATTTTGGCTACTTTTCTTATTCTTCATTGTTCTTTGGATCCGTTGTTGGCAATAGGAGTTCTGATATGTGGAATGATGGCTTCATCACAGTTGATAAAAATATTGAAGGCTCATGTCAAATTTATTCAAATCTTTCAAGGAAACCTTCAAGACTAA

Protein sequence

MLGVNHIIFPRHFTSVHSPVILADLRRTRMPVKVVDAILSDVNADKLQNNKPTFILLLAATSLSLLASVCLKGTWLDIWVDHKFIHYEDGIVDKSITLFITWFIRILATFLILHCSLDPLLAIGVLICGMMASSQLIKILKAHVKFIQIFQGNLQD*

Homology

[Close]

BLAST of CSPI03G29910 vs. ExPASy TrEMBL
Match: A0A1S3CFC0 (uncharacterized protein LOC103500297 OS=Cucumis melo OX=3656 GN=LOC103500297 PE=3 SV=1)

HSP 1 Score: 102.8 bits (255), Expect = 1.3e-18
Identity = 49/82 (59.76%), Postives = 65/82 (79.27%), Query Frame = 0


Query: 60  ATSLSLLASVCLKGTWLDIWVDHKFIHYEDGIVDKSITLFITWFIRILATFLILHCSLDP 119
           A  + LLA + L GTWL   V HK++H++DG+VD +++LF++WFI+ILATFLIL CSLDP
Sbjct: 319 ALKIFLLAFIFLIGTWLGFCVVHKYVHHDDGLVDSTVSLFVSWFIQILATFLILQCSLDP 378

Query: 120 LLAIGVLICGMMASSQLIKILK 142
           LLA GVLICG++ SS L +I K
Sbjct: 379 LLATGVLICGLLTSSMLRRIFK 400

[Close]

BLAST of CSPI03G29910 vs. ExPASy TrEMBL
Match: A0A5D3CHE8 (Nuclear envelope integral membrane protein 1 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold25G00200 PE=3 SV=1)

HSP 1 Score: 96.3 bits (238), Expect = 1.2e-16
Identity = 50/77 (64.94%), Postives = 58/77 (75.32%), Query Frame = 0


Query: 65  LLASVCLKGTWLDIWVDHKFIHYEDGIVDKSITLFITWFIRILATFLILHCSLDPLLAIG 124
           LLA + L G WL  WV HKF+  EDG +D S +LF+TW IRILA+ LIL CSLDPLLA G
Sbjct: 230 LLAFIFLIGAWLGFWVVHKFVLDEDGSIDTSTSLFVTWSIRILASLLILQCSLDPLLATG 289

Query: 125 VLICGMMASSQLIKILK 142
           VLICG++ASS L KI K
Sbjct: 290 VLICGIVASSMLRKIFK 306

[Close]

BLAST of CSPI03G29910 vs. ExPASy TrEMBL
Match: A0A1S4E3G6 (nuclear envelope integral membrane protein 1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103500299 PE=3 SV=1)

HSP 1 Score: 96.3 bits (238), Expect = 1.2e-16
Identity = 50/77 (64.94%), Postives = 58/77 (75.32%), Query Frame = 0


Query: 65  LLASVCLKGTWLDIWVDHKFIHYEDGIVDKSITLFITWFIRILATFLILHCSLDPLLAIG 124
           LLA + L G WL  WV HKF+  EDG +D S +LF+TW IRILA+ LIL CSLDPLLA G
Sbjct: 327 LLAFIFLIGAWLGFWVVHKFVLDEDGSIDTSTSLFVTWSIRILASLLILQCSLDPLLATG 386

Query: 125 VLICGMMASSQLIKILK 142
           VLICG++ASS L KI K
Sbjct: 387 VLICGIVASSMLRKIFK 403

[Close]

BLAST of CSPI03G29910 vs. ExPASy TrEMBL
Match: A0A6J1D6S5 (uncharacterized protein LOC111017862 OS=Momordica charantia OX=3673 GN=LOC111017862 PE=3 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 2.1e-16
Identity = 51/77 (66.23%), Postives = 57/77 (74.03%), Query Frame = 0


Query: 65  LLASVCLKGTWLDIWVDHKFIHYEDGIVDKSITLFITWFIRILATFLILHCSLDPLLAIG 124
           LLA + L G WL  WV HKF+  EDG +  S +LF+TW IRILAT LIL CSLDPLLA G
Sbjct: 265 LLAFIFLIGAWLGFWVVHKFVLDEDGSIHTSTSLFVTWSIRILATLLILQCSLDPLLATG 324

Query: 125 VLICGMMASSQLIKILK 142
           VLICG+MASS L KI K
Sbjct: 325 VLICGVMASSILRKIFK 341

[Close]

BLAST of CSPI03G29910 vs. ExPASy TrEMBL
Match: A0A6J1L1F6 (uncharacterized protein LOC111499541 OS=Cucurbita maxima OX=3661 GN=LOC111499541 PE=3 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 1.0e-15
Identity = 49/77 (63.64%), Postives = 56/77 (72.73%), Query Frame = 0


Query: 65  LLASVCLKGTWLDIWVDHKFIHYEDGIVDKSITLFITWFIRILATFLILHCSLDPLLAIG 124
           LLA + L G WL  WV HKF+  EDG +D S +LF+TW IRILA  LIL CSLDPLLA G
Sbjct: 265 LLAFIFLIGAWLGFWVVHKFVLDEDGSIDTSTSLFVTWCIRILAALLILQCSLDPLLATG 324

Query: 125 VLICGMMASSQLIKILK 142
           VLICG+MASS L +  K
Sbjct: 325 VLICGIMASSILRRTFK 341

[Close]

BLAST of CSPI03G29910 vs. NCBI nr
Match: XP_008461773.1 (PREDICTED: uncharacterized protein LOC103500297 [Cucumis melo])

HSP 1 Score: 102.8 bits (255), Expect = 2.7e-18
Identity = 49/82 (59.76%), Postives = 65/82 (79.27%), Query Frame = 0


Query: 60  ATSLSLLASVCLKGTWLDIWVDHKFIHYEDGIVDKSITLFITWFIRILATFLILHCSLDP 119
           A  + LLA + L GTWL   V HK++H++DG+VD +++LF++WFI+ILATFLIL CSLDP
Sbjct: 319 ALKIFLLAFIFLIGTWLGFCVVHKYVHHDDGLVDSTVSLFVSWFIQILATFLILQCSLDP 378

Query: 120 LLAIGVLICGMMASSQLIKILK 142
           LLA GVLICG++ SS L +I K
Sbjct: 379 LLATGVLICGLLTSSMLRRIFK 400

[Close]

BLAST of CSPI03G29910 vs. NCBI nr
Match: XP_038905972.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC120091886 [Benincasa hispida])

HSP 1 Score: 97.1 bits (240), Expect = 1.5e-16
Identity = 49/73 (67.12%), Postives = 55/73 (75.34%), Query Frame = 0


Query: 69  VCLKGTWLDIWVDHKFIHYEDGIVDKSITLFITWFIRILATFLILHCSLDPLLAIGVLIC 128
           + L GTWL  WV HKF+H EDG +D SI LF+TW IRI AT LIL CSLDPLLA GVLI 
Sbjct: 326 IFLIGTWLGFWVVHKFVHQEDGSIDTSIPLFVTWSIRIFATLLILQCSLDPLLATGVLIS 385

Query: 129 GMMASSQLIKILK 142
           G++ASS L KI K
Sbjct: 386 GIVASSMLRKIFK 398

[Close]

BLAST of CSPI03G29910 vs. NCBI nr
Match: KAA0048313.1 (nuclear envelope integral membrane protein 1 isoform X1 [Cucumis melo var. makuwa] >TYK10658.1 nuclear envelope integral membrane protein 1 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 96.3 bits (238), Expect = 2.5e-16
Identity = 50/77 (64.94%), Postives = 58/77 (75.32%), Query Frame = 0


Query: 65  LLASVCLKGTWLDIWVDHKFIHYEDGIVDKSITLFITWFIRILATFLILHCSLDPLLAIG 124
           LLA + L G WL  WV HKF+  EDG +D S +LF+TW IRILA+ LIL CSLDPLLA G
Sbjct: 230 LLAFIFLIGAWLGFWVVHKFVLDEDGSIDTSTSLFVTWSIRILASLLILQCSLDPLLATG 289

Query: 125 VLICGMMASSQLIKILK 142
           VLICG++ASS L KI K
Sbjct: 290 VLICGIVASSMLRKIFK 306

[Close]

BLAST of CSPI03G29910 vs. NCBI nr
Match: XP_016902769.1 (PREDICTED: nuclear envelope integral membrane protein 1 isoform X1 [Cucumis melo])

HSP 1 Score: 96.3 bits (238), Expect = 2.5e-16
Identity = 50/77 (64.94%), Postives = 58/77 (75.32%), Query Frame = 0


Query: 65  LLASVCLKGTWLDIWVDHKFIHYEDGIVDKSITLFITWFIRILATFLILHCSLDPLLAIG 124
           LLA + L G WL  WV HKF+  EDG +D S +LF+TW IRILA+ LIL CSLDPLLA G
Sbjct: 327 LLAFIFLIGAWLGFWVVHKFVLDEDGSIDTSTSLFVTWSIRILASLLILQCSLDPLLATG 386

Query: 125 VLICGMMASSQLIKILK 142
           VLICG++ASS L KI K
Sbjct: 387 VLICGIVASSMLRKIFK 403

[Close]

BLAST of CSPI03G29910 vs. NCBI nr
Match: XP_022149433.1 (uncharacterized protein LOC111017862 [Momordica charantia])

HSP 1 Score: 95.5 bits (236), Expect = 4.3e-16
Identity = 51/77 (66.23%), Postives = 57/77 (74.03%), Query Frame = 0


Query: 65  LLASVCLKGTWLDIWVDHKFIHYEDGIVDKSITLFITWFIRILATFLILHCSLDPLLAIG 124
           LLA + L G WL  WV HKF+  EDG +  S +LF+TW IRILAT LIL CSLDPLLA G
Sbjct: 265 LLAFIFLIGAWLGFWVVHKFVLDEDGSIHTSTSLFVTWSIRILATLLILQCSLDPLLATG 324

Query: 125 VLICGMMASSQLIKILK 142
           VLICG+MASS L KI K
Sbjct: 325 VLICGVMASSILRKIFK 341

[Close]

BLAST of CSPI03G29910 vs. TAIR 10
Match: AT5G67610.1 (Uncharacterized conserved protein (DUF2215) )

HSP 1 Score: 60.5 bits (145), Expect = 1.4e-09
Identity = 37/81 (45.68%), Postives = 50/81 (61.73%), Query Frame = 0


Query: 67  ASVCLKGTWLDIWVDHKFIHYEDGIVDKSITLFITWFIRILATFLILHCSLDPLLAIGVL 126
           A + L G +   W   K I  EDG +D S +LF++W IRI+A  LIL  S+DPLLA G L
Sbjct: 266 AFLSLGGAFFGFWTVRKLILTEDGSIDVSTSLFVSWSIRIMAAVLILQSSVDPLLAGGAL 325

Query: 127 ICGMMASSQLIKILKAHVKFI 148
           I  ++ SS L KI +  +KF+
Sbjct: 326 ISVILMSSTLKKITR--LKFL 344

[Close]

BLAST of CSPI03G29910 vs. TAIR 10
Match: AT5G67610.2 (Uncharacterized conserved protein (DUF2215) )

HSP 1 Score: 60.5 bits (145), Expect = 1.4e-09
Identity = 37/81 (45.68%), Postives = 50/81 (61.73%), Query Frame = 0


Query: 67  ASVCLKGTWLDIWVDHKFIHYEDGIVDKSITLFITWFIRILATFLILHCSLDPLLAIGVL 126
           A + L G +   W   K I  EDG +D S +LF++W IRI+A  LIL  S+DPLLA G L
Sbjct: 278 AFLSLGGAFFGFWTVRKLILTEDGSIDVSTSLFVSWSIRIMAAVLILQSSVDPLLAGGAL 337

Query: 127 ICGMMASSQLIKILKAHVKFI 148
           I  ++ SS L KI +  +KF+
Sbjct: 338 ISVILMSSTLKKITR--LKFL 356

[Close]

BLAST of CSPI03G29910 vs. TAIR 10
Match: AT3G49840.1 (Uncharacterized conserved protein (DUF2215) )

HSP 1 Score: 59.3 bits (142), Expect = 3.2e-09
Identity = 32/72 (44.44%), Postives = 44/72 (61.11%), Query Frame = 0


Query: 62  SLSLLASVCLKGTWLDIWVDHKFIHYEDGIVDKSITLFITWFIRILATFLILHCSLDPLL 121
           ++ L+  V + G W   W   KF+  +DG VD S ++F++W IR  A  LIL  SLDPLL
Sbjct: 266 AILLVVFVFMLGAWSGFWTVKKFVVTKDGSVDISTSIFVSWSIRAFAVALILQSSLDPLL 325

Query: 122 AIGVLICGMMAS 134
           A G LI G++ S
Sbjct: 326 AGGALITGILIS 337

The following BLAST results are available for this feature:

BLAST of CSPI03G29910 vs.


Match Name	E-value	Identity	Description

BLAST of CSPI03G29910 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3CFC0	1.3e-18	59.76	uncharacterized protein LOC103500297 OS=Cucumis melo OX=3656 GN=LOC103500297 PE=...	[more]
A0A5D3CHE8	1.2e-16	64.94	Nuclear envelope integral membrane protein 1 isoform X1 OS=Cucumis melo var. mak...	[more]
A0A1S4E3G6	1.2e-16	64.94	nuclear envelope integral membrane protein 1 isoform X1 OS=Cucumis melo OX=3656 ...	[more]
A0A6J1D6S5	2.1e-16	66.23	uncharacterized protein LOC111017862 OS=Momordica charantia OX=3673 GN=LOC111017...	[more]
A0A6J1L1F6	1.0e-15	63.64	uncharacterized protein LOC111499541 OS=Cucurbita maxima OX=3661 GN=LOC111499541...	[more]

BLAST of CSPI03G29910 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008461773.1	2.7e-18	59.76	PREDICTED: uncharacterized protein LOC103500297 [Cucumis melo]	[more]
XP_038905972.1	1.5e-16	67.12	LOW QUALITY PROTEIN: uncharacterized protein LOC120091886 [Benincasa hispida]	[more]
KAA0048313.1	2.5e-16	64.94	nuclear envelope integral membrane protein 1 isoform X1 [Cucumis melo var. makuw...	[more]
XP_016902769.1	2.5e-16	64.94	PREDICTED: nuclear envelope integral membrane protein 1 isoform X1 [Cucumis melo...	[more]
XP_022149433.1	4.3e-16	66.23	uncharacterized protein LOC111017862 [Momordica charantia]	[more]

BLAST of CSPI03G29910 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G67610.1	1.4e-09	45.68	Uncharacterized conserved protein (DUF2215)	[more]
AT5G67610.2	1.4e-09	45.68	Uncharacterized conserved protein (DUF2215)	[more]
AT3G49840.1	3.2e-09	44.44	Uncharacterized conserved protein (DUF2215)	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR019358	NEMP family	PFAM	PF10225	NEMP	coord: 73..151 e-value: 3.3E-10 score: 40.0
None	No IPR available	PANTHER	PTHR31587:SF4	TRANSMEMBRANE PROTEIN (DUF2215)	coord: 64..144
None	No IPR available	PANTHER	PTHR31587	TRANSMEMBRANE PROTEIN (DUF2215)	coord: 64..144

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G29910.1	CSPI03G29910.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005637	nuclear inner membrane