CSPI03G29520 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI03G29520
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionRNA polymerase sigma factor sigD
LocationChr3: 27071185 .. 27074717 (+)
RNA-Seq ExpressionCSPI03G29520
SyntenyCSPI03G29520
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AATCCTTCTATCCATTTGCTACAACAAAACTTTGGTGGTTATACAAAAAAAATCCCAAATGCTAAAAAAGAATCCTAACTTTTAAAAGCCCTTTTCCATTCTTCCTTCGTGCCCCTCTTTGCTTATGGCCACTACCAGCAGCTTATGTTCATTACCTGCTCAATCCCCAACTCTCCCATCCATTTCTCTCTCCTCACTTCCATCGCTCAAAACCCATCTTCCCCAACAACCCCATTTCCCCTTTCCCCCTTTCTCCACCAAATTAGGACCCAACATTATCAGCAATGACACATTAACCCTTCCTCCGCCGCCGGAAGCGGCTGGGGTGGCGGTAGAAGCGGCGGGAGAACATGTTCTGGTGGAGGCCAAAGGGTTTTCTGAAGATTGGAGCAGCAAAAATGTTGAGAAAATGGAGGAGAGTTGTGTGGCGGTAGAAGCGGCGGGAGAACATGTTCTGGTGGAGGCCAAAGGGTTTTCTGAAGATTGGAGCAGCAAAAATGTTGAGAAAATGGAGGAGAGTTGTGTGGGTGATGGGACTGTAGCTTGGAAGAAAAAGAGAAGGAAAAGGAGGAAAGAGGTAGGGGTGAAAGCTGAAGAAAATTGGATTGCCTTGTCCAGTGGCCCCTTGAGACCTGGATATTTGAGCCCAAAAGAAGAAGCAGAGCTTTGTCTTTGTCTAAAGGTTGGTTTTAGTAAAGATTTAATGAGTTTTTTTGTCCTTGTCTGTGTCACATTGCCACCATTTACTTCATTAAGGAGCTTACTTGAGGAGATCAGGTAAATGTATGAAAAACACTGCAAGAGTCTCTTCTTTTCCCTTTGTTAATACGTGAATATATTTATTTTCTTCTTTATAAAGTGTTTTTGAGGTATCCTAGAAGAAGAAGAAGAAAGAAGGCTTCTTATTGTGTTGTGGTTTTAAATGCCAGAACTTCAAATTCTTTGTTTTGTTCTCCATTTTGGTTATTGTTGTTAAGTTTTCACATTGTATGCCAAACTCAATGTATGCGGCAGAATGCTAGTCTATATTCTTGAGAGAGGTCAAAATGAAATTGAAAATTTAGAGGCTTAGGAACCAAGATAGATTTAAGTCTTTTATCCTTTAGATCATGTCAAATTATTTGCCACGTTAATATATAAAGGAAAGCTCTGCGTATCTTAAACTCTTAACAGGAGGCAGTACTGCTTGAAAACGTGAAAACTAGAATCATGGAAACTCAAGAGCATGAACCTACAAATAAGCAATTGGCCATTGCAATGGGCACCAAATCAGGGTGTATAGATAGAATTCTTTGTAGAGCAAGAGAATCAAGAGATCGACTCATTCGATGCTATAGCAAACTGGTGATTTCAATTGCTGCTCCATATCAAGGCAAAGGATTAAGCCTTCAAGACCTCACTCAGGTAGGTTGTTGCACGTACTCTTTTGGATTATTGTTTTCTGTGGTTTATTTTGTTCAAAGGGTTTGTTTGTTTTTGTAGGAAGGGAACATTGGTCTTCTTAAAGGAGCAGAAAGATTTGAACCAAATAGAGGACATAAGTTATCAACTTATGCTTATTGGTGGATAAGACAAGCTATCATTCGAGCCTTGGATAAAAAGTCAAGACTTGTTAGACTGCCGGTAAGATACTGACTCAATTGCTCATTTTTCTAATTCTAATTATCATATAGAGGGTCTAGAGAAGAAGCTTCAAATCACAATAAGTTTTTGAGAAAAAGGTAGCATTTTTTAATACATTGCATATGAATACAATCCATAACCTGTTCAGTTAAAAACCTCATCGTAGGCTCTACGTATATTGGTAGGTTTTGGTGACAAGCTTTTTATTGGGTCATATTACTTTATTGGAGCCCTTTTTGTGGTTTACCTCCATTTGTTGACTTTATTTTTCGTATGCACTTGTATTATTTCATCAGGTCAATTGAGAATTTTAACAAAAAAAATGAAAAAGAAAGTTCAGTTGAAAAGATAAACAAAAGTAATGAGCAGCAAACTAAGTTGGATTAATGACAAGACTCATAGAAATCAATAAGGTAATAAGTAAGAGACTTTTGTTATATCACCTTTTAACAAAATGGGGAAAAGTAAATCTGTTTCGAAGATCTGTTTTTTTTTTTTTATTATTATTTATTTCCATAACGTTCTGTTTGCTGAGAAATTTAAGCATGGGAAAGTGAGTTGGTAATAACTGAATGCATGGGTAGCTCGCCGTGTTCAGGGTCACATGGGTCAGATGGTAGCAAGAATAGCAGAAGAGAATAATCATCTTGGCATAAAGTTATCGAGACCTCCCACCAACAAGGAAGTTGCTGAATCTCTTAAAGTCCATCTTTCAACCATAAGACTAGTAATGGAAAGGAGCAAGCGCCCAATTTCAATCAATCAACCAGTAAATGACCGAAGTTGCTTAACTCTGCAGGTACTACTGTTCTCTTACTAGAGGGGATGCCCACATTTTCATTCTTCTTTACGTATTGCACAATGACAACATCTGCAAGGAATGTCACGTATGCAATTGCCTTTTGATCTTTAGGAATCAGGATTTACGATTTTAGTCTCTTTAATGTTCATAAAATTAGTAATCAATGACATTTCCCACATGCTCATCTACAAGTATAACTAAAGTAATGCCATGCTCTTATCTCCTCTGTTCAACATTTCCAAACGAGAGGTTGAACATCAAATGGATTGCATACCAATAAGATTGGTATACTTGGGGTTTGCTTATTAATATTGGAACGAACTAAGTTGTTAGAAGAAGTTCAAATAATTTTATTTGGCAACTGCCTTAATCCTAGGTAGATAGCTACCACAGAAAATAGTAGTTAACTTCTATGGTTATGTTTTTGTTTCAGTGACTAAGATATGATGTTGAAACAATTCTTTGTCAATGTTGTGCAGGAGATCATGCCTGGACCAGACAATTTGACACCAGAGAATATGGTAATGAGAAAATTGATGAAACAAGAACTGAAGAGGCTTCTTAATACTCTTAGCAAAAGAGAAGCACGCATATTGAGCTTATACTTTGGCCTCAATGGAGAGATTCCTCAGTCCTTTGAGGAGATAGGCAAGTCATTGAATCTCTCTCGAGAGACAATTCGACAAACCAATATTGCCACATTGTCAAAACTAAAGAAGACCGACGTTCTAGACTATCTTAAAGATTATCTAGTGTAGATATTTCAAGCTATGATAAAAAGTTACAAGTAAAGATTTGATTGGATAGACTATTGAAGGATGTTGAAGAGTATTAATTGTATGCATCAAGATTTAAATCTATCTTTGGTACATCAAAGGAGTTATATGGGCTGCAAACAGTTGAAACGGATTTGATCGATATATGTAGAAGATCGATCGAGTAACTGGTCTTTTGTTTGCTGGTGTAAATAAGAAATAAGAAACCAAATCAATTGATGTTTACTTGATGTATTGCCTTGTTTTGTAAAATTAAACATGTTGACTTAAAAGTTTATGATACAATTGATAGTTGCTTGTAGACACTGTATCGACTAAGTGCATAATCCTGG

mRNA sequence

AATCCTTCTATCCATTTGCTACAACAAAACTTTGGTGGTTATACAAAAAAAATCCCAAATGCTAAAAAAGAATCCTAACTTTTAAAAGCCCTTTTCCATTCTTCCTTCGTGCCCCTCTTTGCTTATGGCCACTACCAGCAGCTTATGTTCATTACCTGCTCAATCCCCAACTCTCCCATCCATTTCTCTCTCCTCACTTCCATCGCTCAAAACCCATCTTCCCCAACAACCCCATTTCCCCTTTCCCCCTTTCTCCACCAAATTAGGACCCAACATTATCAGCAATGACACATTAACCCTTCCTCCGCCGCCGGAAGCGGCTGGGGTGGCGGTAGAAGCGGCGGGAGAACATGTTCTGGTGGAGGCCAAAGGGTTTTCTGAAGATTGGAGCAGCAAAAATGTTGAGAAAATGGAGGAGAGTTGTGTGGCGGTAGAAGCGGCGGGAGAACATGTTCTGGTGGAGGCCAAAGGGTTTTCTGAAGATTGGAGCAGCAAAAATGTTGAGAAAATGGAGGAGAGTTGTGTGGGTGATGGGACTGTAGCTTGGAAGAAAAAGAGAAGGAAAAGGAGGAAAGAGGTAGGGGTGAAAGCTGAAGAAAATTGGATTGCCTTGTCCAGTGGCCCCTTGAGACCTGGATATTTGAGCCCAAAAGAAGAAGCAGAGCTTTGTCTTTGTCTAAAGGAGGCAGTACTGCTTGAAAACGTGAAAACTAGAATCATGGAAACTCAAGAGCATGAACCTACAAATAAGCAATTGGCCATTGCAATGGGCACCAAATCAGGGTGTATAGATAGAATTCTTTGTAGAGCAAGAGAATCAAGAGATCGACTCATTCGATGCTATAGCAAACTGGTGATTTCAATTGCTGCTCCATATCAAGGCAAAGGATTAAGCCTTCAAGACCTCACTCAGGAAGGGAACATTGGTCTTCTTAAAGGAGCAGAAAGATTTGAACCAAATAGAGGACATAAGTTATCAACTTATGCTTATTGGTGGATAAGACAAGCTATCATTCGAGCCTTGGATAAAAAGTCAAGACTTGTTAGACTGCCGGGTCACATGGGTCAGATGGTAGCAAGAATAGCAGAAGAGAATAATCATCTTGGCATAAAGTTATCGAGACCTCCCACCAACAAGGAAGTTGCTGAATCTCTTAAAGTCCATCTTTCAACCATAAGACTAGTAATGGAAAGGAGCAAGCGCCCAATTTCAATCAATCAACCAGTAAATGACCGAAGTTGCTTAACTCTGCAGGAGATCATGCCTGGACCAGACAATTTGACACCAGAGAATATGGTAATGAGAAAATTGATGAAACAAGAACTGAAGAGGCTTCTTAATACTCTTAGCAAAAGAGAAGCACGCATATTGAGCTTATACTTTGGCCTCAATGGAGAGATTCCTCAGTCCTTTGAGGAGATAGGCAAGTCATTGAATCTCTCTCGAGAGACAATTCGACAAACCAATATTGCCACATTGTCAAAACTAAAGAAGACCGACGTTCTAGACTATCTTAAAGATTATCTAGTGTAGATATTTCAAGCTATGATAAAAAGTTACAAGTAAAGATTTGATTGGATAGACTATTGAAGGATGTTGAAGAGTATTAATTGTATGCATCAAGATTTAAATCTATCTTTGGTACATCAAAGGAGTTATATGGGCTGCAAACAGTTGAAACGGATTTGATCGATATATGTAGAAGATCGATCGAGTAACTGGTCTTTTGTTTGCTGGTGTAAATAAGAAATAAGAAACCAAATCAATTGATGTTTACTTGATGTATTGCCTTGTTTTGTAAAATTAAACATGTTGACTTAAAAGTTTATGATACAATTGATAGTTGCTTGTAGACACTGTATCGACTAAGTGCATAATCCTGG

Coding sequence (CDS)

ATGGCCACTACCAGCAGCTTATGTTCATTACCTGCTCAATCCCCAACTCTCCCATCCATTTCTCTCTCCTCACTTCCATCGCTCAAAACCCATCTTCCCCAACAACCCCATTTCCCCTTTCCCCCTTTCTCCACCAAATTAGGACCCAACATTATCAGCAATGACACATTAACCCTTCCTCCGCCGCCGGAAGCGGCTGGGGTGGCGGTAGAAGCGGCGGGAGAACATGTTCTGGTGGAGGCCAAAGGGTTTTCTGAAGATTGGAGCAGCAAAAATGTTGAGAAAATGGAGGAGAGTTGTGTGGCGGTAGAAGCGGCGGGAGAACATGTTCTGGTGGAGGCCAAAGGGTTTTCTGAAGATTGGAGCAGCAAAAATGTTGAGAAAATGGAGGAGAGTTGTGTGGGTGATGGGACTGTAGCTTGGAAGAAAAAGAGAAGGAAAAGGAGGAAAGAGGTAGGGGTGAAAGCTGAAGAAAATTGGATTGCCTTGTCCAGTGGCCCCTTGAGACCTGGATATTTGAGCCCAAAAGAAGAAGCAGAGCTTTGTCTTTGTCTAAAGGAGGCAGTACTGCTTGAAAACGTGAAAACTAGAATCATGGAAACTCAAGAGCATGAACCTACAAATAAGCAATTGGCCATTGCAATGGGCACCAAATCAGGGTGTATAGATAGAATTCTTTGTAGAGCAAGAGAATCAAGAGATCGACTCATTCGATGCTATAGCAAACTGGTGATTTCAATTGCTGCTCCATATCAAGGCAAAGGATTAAGCCTTCAAGACCTCACTCAGGAAGGGAACATTGGTCTTCTTAAAGGAGCAGAAAGATTTGAACCAAATAGAGGACATAAGTTATCAACTTATGCTTATTGGTGGATAAGACAAGCTATCATTCGAGCCTTGGATAAAAAGTCAAGACTTGTTAGACTGCCGGGTCACATGGGTCAGATGGTAGCAAGAATAGCAGAAGAGAATAATCATCTTGGCATAAAGTTATCGAGACCTCCCACCAACAAGGAAGTTGCTGAATCTCTTAAAGTCCATCTTTCAACCATAAGACTAGTAATGGAAAGGAGCAAGCGCCCAATTTCAATCAATCAACCAGTAAATGACCGAAGTTGCTTAACTCTGCAGGAGATCATGCCTGGACCAGACAATTTGACACCAGAGAATATGGTAATGAGAAAATTGATGAAACAAGAACTGAAGAGGCTTCTTAATACTCTTAGCAAAAGAGAAGCACGCATATTGAGCTTATACTTTGGCCTCAATGGAGAGATTCCTCAGTCCTTTGAGGAGATAGGCAAGTCATTGAATCTCTCTCGAGAGACAATTCGACAAACCAATATTGCCACATTGTCAAAACTAAAGAAGACCGACGTTCTAGACTATCTTAAAGATTATCTAGTGTAG

Protein sequence

MATTSSLCSLPAQSPTLPSISLSSLPSLKTHLPQQPHFPFPPFSTKLGPNIISNDTLTLPPPPEAAGVAVEAAGEHVLVEAKGFSEDWSSKNVEKMEESCVAVEAAGEHVLVEAKGFSEDWSSKNVEKMEESCVGDGTVAWKKKRRKRRKEVGVKAEENWIALSSGPLRPGYLSPKEEAELCLCLKEAVLLENVKTRIMETQEHEPTNKQLAIAMGTKSGCIDRILCRARESRDRLIRCYSKLVISIAAPYQGKGLSLQDLTQEGNIGLLKGAERFEPNRGHKLSTYAYWWIRQAIIRALDKKSRLVRLPGHMGQMVARIAEENNHLGIKLSRPPTNKEVAESLKVHLSTIRLVMERSKRPISINQPVNDRSCLTLQEIMPGPDNLTPENMVMRKLMKQELKRLLNTLSKREARILSLYFGLNGEIPQSFEEIGKSLNLSRETIRQTNIATLSKLKKTDVLDYLKDYLV*
Homology
BLAST of CSPI03G29520 vs. ExPASy Swiss-Prot
Match: Q9ZSL6 (RNA polymerase sigma factor sigD, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=SIGD PE=2 SV=1)

HSP 1 Score: 321.2 bits (822), Expect = 1.9e-86
Identity = 171/345 (49.57%), Postives = 238/345 (68.99%), Query Frame = 0

Query: 137 GTVAWKKKRRKRRKEV-------------GVKAEENWIALS-SGPLRPGYLSPKEEAELC 196
           G  + K+++R+RR+ V             GV+AE   I++   G  R G+LS  EE +LC
Sbjct: 73  GIQSEKRRKRRRRRRVGYERLEPEEEENAGVEAEAETISVPVVGASRSGFLSRLEEVQLC 132

Query: 197 LCLKEAVLLENVKTRIMETQEHEPTNKQLAIAMGTKSGCIDRILCRARESRDRLIRCYSK 256
           L LKE   LEN+ T +   +E+E  +  LA   G K    + ILCR +E+R+++ RCY +
Sbjct: 133 LYLKEGAKLENLGTSV---EENEMVSVLLASGRGKKKRSANEILCRRKEAREKITRCYRR 192

Query: 257 LVISIAAPYQGKGLSLQDLTQEGNIGLLKGAERFEPNRGHKLSTYAYWWIRQAIIRALDK 316
           LV+SIA  YQGKGL+LQDL QEG+IGLL+GAERF+P+RG+KLSTY YWWI+QAI+RA+  
Sbjct: 193 LVVSIATGYQGKGLNLQDLIQEGSIGLLRGAERFDPDRGYKLSTYVYWWIKQAILRAIAH 252

Query: 317 KSRLVRLPGHMGQMVARIAEENNHLGIKLSRPPTNKEVAESLKVHLSTIRLVMERSKRPI 376
           KSRLV+LPG M ++ A++AE +N L  KL R P+ +E+AE L +++S +RL +ERS+ P+
Sbjct: 253 KSRLVKLPGSMWELTAKVAEASNVLTRKLRRQPSCEEIAEHLNLNVSAVRLAVERSRSPV 312

Query: 377 SINQPVNDRSCLTLQEIMPGPDNLTPENMVMRKLMKQELKRLLNTLSKREARILSLYFGL 436
           S+++  +    +TLQEI+ GPD   PE MV R+ MK E+++LL +L+ RE+R+L LYFGL
Sbjct: 313 SLDRVASQNGRMTLQEIVRGPDETRPEEMVKREHMKHEIEQLLGSLTARESRVLGLYFGL 372

Query: 437 NGEIPQSFEEIGKSLNLSRETIRQTNIATLSKLKKTDVLDYLKDY 468
           NGE P SFEEIGKSL LSRE +RQ N   L KL+    ++ LK Y
Sbjct: 373 NGETPMSFEEIGKSLKLSRERVRQINGIALKKLRNVHNVNDLKIY 414

BLAST of CSPI03G29520 vs. ExPASy Swiss-Prot
Match: P38023 (RNA polymerase sigma factor SigA1 OS=Synechococcus elongatus (strain PCC 7942 / FACHB-805) OX=1140 GN=sigA1 PE=3 SV=2)

HSP 1 Score: 199.9 bits (507), Expect = 6.4e-50
Identity = 116/340 (34.12%), Postives = 197/340 (57.94%), Query Frame = 0

Query: 130 EESCVGDGTVAWKKKRRKRRKEVGVKAEENWIALSSGPL-RPGYLSPKEEAELCLCLKEA 189
           +E  VG+   A  K + K RK       E+ I L    + R   L   EE EL   + + 
Sbjct: 64  DEDSVGEDEDAAAKAKAKVRKTY----TEDSIRLYLQEIGRIRLLRADEEIELARQIADL 123

Query: 190 VLLENVKTRIMETQEHEPTNKQLAIAMGTKSGCIDRILCRARESRDRLIRCYSKLVISIA 249
           + LE ++  ++E  +  P++ + A A+ +      R L R R ++D++++   +LV+SIA
Sbjct: 124 LALERIRDELLEQLDRLPSDAEWAAAVDSPLDEFRRRLFRGRRAKDKMVQSNLRLVVSIA 183

Query: 250 APYQGKGLSLQDLTQEGNIGLLKGAERFEPNRGHKLSTYAYWWIRQAIIRALDKKSRLVR 309
             Y  +GLS QDL QEG++GL++ AE+F+  +G+K STYA WWIRQAI RA+  +SR +R
Sbjct: 184 KKYMNRGLSFQDLIQEGSLGLIRAAEKFDHEKGYKFSTYATWWIRQAITRAIADQSRTIR 243

Query: 310 LPGHMGQMVARIAEENNHLGIKLSRPPTNKEVAESLKVHLSTIRLVMERSKRPISINQPV 369
           LP H+ + ++RI +    L  ++ R PT +E+A  +++ +  +R + + ++ PIS+  P+
Sbjct: 244 LPVHLYETISRIKKTTKLLSQEMGRKPTEEEIATRMEMTIEKLRFIAKSAQLPISLETPI 303

Query: 370 NDRSCLTLQEIMPGPDNLTPENMVMRKLMKQELKRLLNTLSKREARILSLYFGLNGEIPQ 429
                  L + +   D  TPE+ V + L++++L+ +L+TLS RE  +L L +GL+    +
Sbjct: 304 GKEEDSRLGDFIEA-DGETPEDEVAKNLLREDLEGVLSTLSPRERDVLRLRYGLDDGRMK 363

Query: 430 SFEEIGKSLNLSRETIRQTNIATLSKLKKTDVLDYLKDYL 469
           + EEIG+  N++RE IRQ     L KL+  +    LK+Y+
Sbjct: 364 TLEEIGQLFNVTRERIRQIEAKALRKLRHPNRNSILKEYI 398

BLAST of CSPI03G29520 vs. ExPASy Swiss-Prot
Match: P26683 (RNA polymerase sigma factor SigA OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) OX=103690 GN=sigA PE=3 SV=1)

HSP 1 Score: 196.1 bits (497), Expect = 9.2e-49
Identity = 111/339 (32.74%), Postives = 193/339 (56.93%), Query Frame = 0

Query: 130 EESCVGDGTVAWKKKRRKRRKEVGVKAEENWIALSSGPLRPGYLSPKEEAELCLCLKEAV 189
           +E     G  A  ++R + +K+   +          G +R   L   EE EL   + + +
Sbjct: 54  DEDDAKSGKAAKSRRRTQSKKKHYTEDSIRLYLQEIGRIR--LLRADEEIELARKIADLL 113

Query: 190 LLENVKTRIMETQEHEPTNKQLAIAMGTKSGCIDRILCRARESRDRLIRCYSKLVISIAA 249
            LE V+ R+ E  E +P + + A A+          L   R ++D++++   +LV+SIA 
Sbjct: 114 ELERVRERLSEKLERDPRDSEWAEAVQLPLPAFRYRLHIGRRAKDKMVQSNLRLVVSIAK 173

Query: 250 PYQGKGLSLQDLTQEGNIGLLKGAERFEPNRGHKLSTYAYWWIRQAIIRALDKKSRLVRL 309
            Y  +GLS QDL QEG++GL++ AE+F+  +G+K STYA WWIRQAI RA+  +SR +RL
Sbjct: 174 KYMNRGLSFQDLIQEGSLGLIRAAEKFDHEKGYKFSTYATWWIRQAITRAIADQSRTIRL 233

Query: 310 PGHMGQMVARIAEENNHLGIKLSRPPTNKEVAESLKVHLSTIRLVMERSKRPISINQPVN 369
           P H+ + ++RI +    L  ++ R PT +E+A  +++ +  +R + + ++ PIS+  P+ 
Sbjct: 234 PVHLYETISRIKKTTKLLSQEMGRKPTEEEIATRMEMTIEKLRFIAKSAQLPISLETPIG 293

Query: 370 DRSCLTLQEIMPGPDNLTPENMVMRKLMKQELKRLLNTLSKREARILSLYFGLNGEIPQS 429
                 L + +   D  TPE+ V + L++++L+++L++LS RE  +L L +GL+    ++
Sbjct: 294 KEEDSRLGDFIES-DGETPEDQVSKNLLREDLEKVLDSLSPRERDVLRLRYGLDDGRMKT 353

Query: 430 FEEIGKSLNLSRETIRQTNIATLSKLKKTDVLDYLKDYL 469
            EEIG+  N++RE IRQ     L KL+  +    LK+Y+
Sbjct: 354 LEEIGQIFNVTRERIRQIEAKALRKLRHPNRNSVLKEYI 389

BLAST of CSPI03G29520 vs. ExPASy Swiss-Prot
Match: O22056 (RNA polymerase sigma factor sigB OS=Arabidopsis thaliana OX=3702 GN=SIGB PE=2 SV=2)

HSP 1 Score: 194.9 bits (494), Expect = 2.0e-48
Identity = 120/390 (30.77%), Postives = 212/390 (54.36%), Query Frame = 0

Query: 80  EAKGFSEDWSSKNVEKMEESCVAVEAAGEHVLVEAKGFSEDWSSKNVEKMEESCVGDGTV 139
           E+ G  E    + VE +EE      A       E K       +K +EK         T 
Sbjct: 186 ESIGDLESEKQEEVELLEEQPSVSLAVRSTRQTERKA----RRAKGLEKTASGIPSVKTG 245

Query: 140 AWKKKRRKRRKEVGVKAEENWIALSSGPLRPGYLSPKEEAELCLCLKEAVLLENVKTRIM 199
           +  KK+R   +EV       ++ +++   +   L+ +EE EL   +++ + LE ++T + 
Sbjct: 246 SSPKKKRLVAQEVDHNDPLRYLRMTTSSSK--LLTVREEHELSAGIQDLLKLERLQTELT 305

Query: 200 ETQEHEPTNKQLAIAMGTKSGCIDRILCRARESRDRLIRCYSKLVISIAAPYQGKGLSLQ 259
           E    +PT  Q A A G     + + +      +D++I+   +LVISIA  YQG G++LQ
Sbjct: 306 ERSGRQPTFAQWASAAGVDQKSLRQRIHHGTLCKDKMIKSNIRLVISIAKNYQGAGMNLQ 365

Query: 260 DLTQEGNIGLLKGAERFEPNRGHKLSTYAYWWIRQAIIRALDKKSRLVRLPGHMGQMVAR 319
           DL QEG  GL++GAE+F+  +G K STYA+WWI+QA+ ++L  +SR++RLP HM +   R
Sbjct: 366 DLVQEGCRGLVRGAEKFDATKGFKFSTYAHWWIKQAVRKSLSDQSRMIRLPFHMVEATYR 425

Query: 320 IAEENNHLGIKLSRPPTNKEVAESLKVHLSTIRLVMERSKRPISINQPVNDRSCLTLQEI 379
           + E    L  +  + P N+E+AE+  + +  +  V+   K P S++Q +     L   E+
Sbjct: 426 VKEARKQLYSETGKHPKNEEIAEATGLSMKRLMAVLLSPKPPRSLDQKIGMNQNLKPSEV 485

Query: 380 MPGPDNLTPENMVMRKLMKQELKRLLNTLSKREARILSLYFGLNGEIPQSFEEIGKSLNL 439
           +  P+ +T E++++++ M+Q+L ++L++L  RE +++   FG+     ++ +EIG+ + +
Sbjct: 486 IADPEAVTSEDILIKEFMRQDLDKVLDSLGTREKQVIRWRFGMEDGRMKTLQEIGEMMGV 545

Query: 440 SRETIRQTNIATLSKLKKTDVLDYLKDYLV 470
           SRE +RQ   +   KLK     ++L+ YLV
Sbjct: 546 SRERVRQIESSAFRKLKNKKRNNHLQQYLV 569

BLAST of CSPI03G29520 vs. ExPASy Swiss-Prot
Match: Q9LD95 (RNA polymerase sigma factor sigF, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=SIGF PE=1 SV=1)

HSP 1 Score: 194.5 bits (493), Expect = 2.7e-48
Identity = 114/335 (34.03%), Postives = 187/335 (55.82%), Query Frame = 0

Query: 123 SKNVEKMEESCVGDGTVAWKKKRRKRRKEVGVKAEENWIALSSGPLRPGYLSPKEEAELC 182
           +KN    + + V D     +K   K++ + G   ++       GP     L+ KEEAEL 
Sbjct: 198 AKNRRAPKSNDVDDEGYVPQKTSAKKKYKQGADNDDALQLFLWGPETKQLLTAKEEAELI 257

Query: 183 LCLKEAVLLENVKTRIMETQEHEPTNKQLAIAMGTKSGCIDRILCRARESRDRLIRCYSK 242
             ++  + LE VKT++      EPT  + A AMG  S  +   + R R SR++LI    +
Sbjct: 258 SHIQHLLKLEKVKTKLESQNGCEPTIGEWAEAMGISSPVLKSDIHRGRSSREKLITANLR 317

Query: 243 LVISIAAPYQGKGLSLQDLTQEGNIGLLKGAERFEPNRGHKLSTYAYWWIRQAIIRALDK 302
           LV+ IA  YQ +GL+ QDL QEG++GL+K  E+F+P  G + +TYAYWWIRQ+I +++ +
Sbjct: 318 LVVHIAKQYQNRGLNFQDLLQEGSMGLMKSVEKFKPQSGCRFATYAYWWIRQSIRKSIFQ 377

Query: 303 KSRLVRLPGHMGQMVARIAEENNHLGIKLSRPPTNKEVAESLKVHLSTIRLVMERSKRPI 362
            SR +RLP ++  ++ +++E       + +  P+ +E+A  + V    +  ++  ++ P+
Sbjct: 378 NSRTIRLPENVYMLLGKVSEARKTCVQEGNYRPSKEELAGHVGVSTEKLDKLLYNTRTPL 437

Query: 363 SINQPVNDRSCLTLQEIMPGPDNLTPENMVMRKLMKQELKRLLNTLSKREARILSLYFGL 422
           S+ QP+      T QEI P     TP   V ++LM+  ++ LLN LS +E RI+ L FG+
Sbjct: 438 SMQQPIWSDQDTTFQEITPDSGIETPTMSVGKQLMRNHVRNLLNVLSPKERRIIKLRFGI 497

Query: 423 NGEIPQSFEEIGKSLNLSRETIRQTNIATLSKLKK 458
           +G   +S  EIG+   LS+E +RQ     L +LK+
Sbjct: 498 DGGKQRSLSEIGEIYGLSKERVRQLESRALYRLKQ 532

BLAST of CSPI03G29520 vs. ExPASy TrEMBL
Match: A0A0A0L9T0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G687740 PE=3 SV=1)

HSP 1 Score: 833.9 bits (2153), Expect = 3.2e-238
Identity = 434/469 (92.54%), Postives = 435/469 (92.75%), Query Frame = 0

Query: 1   MATTSSLCSLPAQSPTLPSISLSSLPSLKTHLPQQPHFPFPPFSTKLGPNIISNDTLTLP 60
           MATTSSLCSLPAQSPTLPSISLSSLPSLKTHLPQQPHFPFPPFSTKLGPNIISNDTLTLP
Sbjct: 1   MATTSSLCSLPAQSPTLPSISLSSLPSLKTHLPQQPHFPFPPFSTKLGPNIISNDTLTLP 60

Query: 61  PPPEAAGVAVEAAGEHVLVEAKGFSEDWSSKNVEKMEESCVAVEAAGEHVLVEAKGFSED 120
           PPPEAA                                  +AVEAAGEHVLVEAKGFSED
Sbjct: 61  PPPEAA---------------------------------VLAVEAAGEHVLVEAKGFSED 120

Query: 121 WSSKNVEKMEESCVGDGTVAWKKKRRKRRKEVGVKAEENWIALSSGPLRPGYLSPKEEAE 180
           WSSKNVEKMEESCVGDGTVAWKKKRRKRRKEVGVKAEENWIALSSGPLRPGYLSPKEEAE
Sbjct: 121 WSSKNVEKMEESCVGDGTVAWKKKRRKRRKEVGVKAEENWIALSSGPLRPGYLSPKEEAE 180

Query: 181 LCLCLKEAVLLENVKTRIMETQEHEPTNKQLAIAMGTKSGCIDRILCRARESRDRLIRCY 240
           LCLCLKEAVLLENVKTRIMETQEHEPTNKQLAIAMGTKSGCIDRILCRARESRDRLIRCY
Sbjct: 181 LCLCLKEAVLLENVKTRIMETQEHEPTNKQLAIAMGTKSGCIDRILCRARESRDRLIRCY 240

Query: 241 SKLVISIAAPYQGKGLSLQDLTQEGNIGLLKGAERFEPNRGHKLSTYAYWWIRQAIIRAL 300
           SKLVISIAAPYQGKGLSLQDLTQEGNIGLLKGAERFEPNRGHKLSTYAYWWIRQAIIRAL
Sbjct: 241 SKLVISIAAPYQGKGLSLQDLTQEGNIGLLKGAERFEPNRGHKLSTYAYWWIRQAIIRAL 300

Query: 301 DKKSRLVRLPGHMGQMVARIAEENNHLGIKLSRPPTNKEVAESLKVHLSTIRLVMERSKR 360
           DKKSRLVRLPGHMGQMVARIAEENNHLGIKLSRPPTNKEVAESLKVHLSTIRLVMERSKR
Sbjct: 301 DKKSRLVRLPGHMGQMVARIAEENNHLGIKLSRPPTNKEVAESLKVHLSTIRLVMERSKR 360

Query: 361 PISINQPVNDRSCLTLQEIMPGPDNLTPENMVMRKLMKQELKRLLNTLSKREARILSLYF 420
           PISINQPVNDRSCLTLQEIMPGPDNLTPENMVMRKLMKQELKRLLNTLSKREARILSLYF
Sbjct: 361 PISINQPVNDRSCLTLQEIMPGPDNLTPENMVMRKLMKQELKRLLNTLSKREARILSLYF 420

Query: 421 GLNGEIPQSFEEIGKSLNLSRETIRQTNIATLSKLKKTDVLDYLKDYLV 470
           GLNGEIPQSFEEIGKSLNLSRETIRQTNIATLSKLKKTDVLDYLKDYLV
Sbjct: 421 GLNGEIPQSFEEIGKSLNLSRETIRQTNIATLSKLKKTDVLDYLKDYLV 436

BLAST of CSPI03G29520 vs. ExPASy TrEMBL
Match: A0A5D3BP27 (RNA polymerase sigma factor sigD OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold474G00100 PE=3 SV=1)

HSP 1 Score: 801.6 bits (2069), Expect = 1.8e-228
Identity = 415/469 (88.49%), Postives = 428/469 (91.26%), Query Frame = 0

Query: 1   MATTSSLCSLPAQSPTLPSISLSSLPSLKTHLPQQPHFPFPPFSTKLGPNIISNDTLTLP 60
           MATTSSLCSLPAQSPTLPSISLSSLPSLKTHLPQQPHFPFPPFS+KLGPN IS DTLTLP
Sbjct: 1   MATTSSLCSLPAQSPTLPSISLSSLPSLKTHLPQQPHFPFPPFSSKLGPNFISYDTLTLP 60

Query: 61  PPPEAAGVAVEAAGEHVLVEAKGFSEDWSSKNVEKMEESCVAVEAAGEHVLVEAKGFSED 120
           PPPEAA                                  +AVEAAGEHVLVEA+GFSED
Sbjct: 61  PPPEAA---------------------------------VLAVEAAGEHVLVEARGFSED 120

Query: 121 WSSKNVEKMEESCVGDGTVAWKKKRRKRRKEVGVKAEENWIALSSGPLRPGYLSPKEEAE 180
           WSSKNVE+MEESCVGDGTVAWKKKRRKRRKEVGVKAEENWIALSSGPLRPGYLSPKEEAE
Sbjct: 121 WSSKNVERMEESCVGDGTVAWKKKRRKRRKEVGVKAEENWIALSSGPLRPGYLSPKEEAE 180

Query: 181 LCLCLKEAVLLENVKTRIMETQEHEPTNKQLAIAMGTKSGCIDRILCRARESRDRLIRCY 240
           LCLCLKEAVLLE VK+RIMETQEHEPTNKQLAIAMGTK GCIDRILCRARESRDRLIRCY
Sbjct: 181 LCLCLKEAVLLEKVKSRIMETQEHEPTNKQLAIAMGTKPGCIDRILCRARESRDRLIRCY 240

Query: 241 SKLVISIAAPYQGKGLSLQDLTQEGNIGLLKGAERFEPNRGHKLSTYAYWWIRQAIIRAL 300
           SKLVISIAAPYQGKGLSLQDLTQEGNIGLLKGAERFEP+RGHKLSTYAYWWIRQAIIRAL
Sbjct: 241 SKLVISIAAPYQGKGLSLQDLTQEGNIGLLKGAERFEPSRGHKLSTYAYWWIRQAIIRAL 300

Query: 301 DKKSRLVRLPGHMGQMVARIAEENNHLGIKLSRPPTNKEVAESLKVHLSTIRLVMERSKR 360
           DKKSRLVRLPGHMGQMVARIAEENNHLGIKLSRPPTN+EVAESLKV +ST+RLVMERSKR
Sbjct: 301 DKKSRLVRLPGHMGQMVARIAEENNHLGIKLSRPPTNEEVAESLKVRVSTVRLVMERSKR 360

Query: 361 PISINQPVNDRSCLTLQEIMPGPDNLTPENMVMRKLMKQELKRLLNTLSKREARILSLYF 420
           PISINQPVNDRSCLTLQEI+PGPDNLTPENMVMRKLMKQELKRLLNTLSKREA ILSLYF
Sbjct: 361 PISINQPVNDRSCLTLQEIIPGPDNLTPENMVMRKLMKQELKRLLNTLSKREAHILSLYF 420

Query: 421 GLNGEIPQSFEEIGKSLNLSRETIRQTNIATLSKLKKTDVLDYLKDYLV 470
           GLNGEIPQSFEEIGKSLNLSRETIR+ NIATLSKLKKT+VLDY+KDYLV
Sbjct: 421 GLNGEIPQSFEEIGKSLNLSRETIRRINIATLSKLKKTNVLDYIKDYLV 436

BLAST of CSPI03G29520 vs. ExPASy TrEMBL
Match: A0A1S3CFL7 (RNA polymerase sigma factor sigD, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103500331 PE=3 SV=1)

HSP 1 Score: 779.6 bits (2012), Expect = 7.2e-222
Identity = 402/456 (88.16%), Postives = 415/456 (91.01%), Query Frame = 0

Query: 14  SPTLPSISLSSLPSLKTHLPQQPHFPFPPFSTKLGPNIISNDTLTLPPPPEAAGVAVEAA 73
           SPTLPSISLSSLPSLKTHLPQQPHFPFPPFS+KLGPN IS DTLTLPPPPEAA       
Sbjct: 51  SPTLPSISLSSLPSLKTHLPQQPHFPFPPFSSKLGPNFISYDTLTLPPPPEAA------- 110

Query: 74  GEHVLVEAKGFSEDWSSKNVEKMEESCVAVEAAGEHVLVEAKGFSEDWSSKNVEKMEESC 133
                                      +AVEAAGEHVLVEA+GFSEDWSSKNVE+MEESC
Sbjct: 111 --------------------------VLAVEAAGEHVLVEARGFSEDWSSKNVERMEESC 170

Query: 134 VGDGTVAWKKKRRKRRKEVGVKAEENWIALSSGPLRPGYLSPKEEAELCLCLKEAVLLEN 193
           VGDGTVAWKKKRRKRRKEVGVKAEENWIALSSGPLRPGYLSPKEEAELCLCLKEAVLLE 
Sbjct: 171 VGDGTVAWKKKRRKRRKEVGVKAEENWIALSSGPLRPGYLSPKEEAELCLCLKEAVLLEK 230

Query: 194 VKTRIMETQEHEPTNKQLAIAMGTKSGCIDRILCRARESRDRLIRCYSKLVISIAAPYQG 253
           VK+RIMETQEHEPTNKQLAIAMGTK GCIDRILCRARESRDRLIRCYSKLVISIAAPYQG
Sbjct: 231 VKSRIMETQEHEPTNKQLAIAMGTKPGCIDRILCRARESRDRLIRCYSKLVISIAAPYQG 290

Query: 254 KGLSLQDLTQEGNIGLLKGAERFEPNRGHKLSTYAYWWIRQAIIRALDKKSRLVRLPGHM 313
           KGLSLQDLTQEGNIGLLKGAERFEP+RGHKLSTYAYWWIRQAIIRALDKKSRLVRLPGHM
Sbjct: 291 KGLSLQDLTQEGNIGLLKGAERFEPSRGHKLSTYAYWWIRQAIIRALDKKSRLVRLPGHM 350

Query: 314 GQMVARIAEENNHLGIKLSRPPTNKEVAESLKVHLSTIRLVMERSKRPISINQPVNDRSC 373
           GQMVARIAEENNHLGIKLSRPPTN+EVAESLKV +ST+RLVMERSKRPISINQPVNDRSC
Sbjct: 351 GQMVARIAEENNHLGIKLSRPPTNEEVAESLKVRVSTVRLVMERSKRPISINQPVNDRSC 410

Query: 374 LTLQEIMPGPDNLTPENMVMRKLMKQELKRLLNTLSKREARILSLYFGLNGEIPQSFEEI 433
           LTLQEI+PGPDNLTPENMVMRKLMKQELKRLLNTLSKREA ILSLYFGLNGEIPQSFEEI
Sbjct: 411 LTLQEIIPGPDNLTPENMVMRKLMKQELKRLLNTLSKREAHILSLYFGLNGEIPQSFEEI 470

Query: 434 GKSLNLSRETIRQTNIATLSKLKKTDVLDYLKDYLV 470
           GKSLNLSRETIR+ NIATLSKLKKT+VLDY+KDYLV
Sbjct: 471 GKSLNLSRETIRRINIATLSKLKKTNVLDYIKDYLV 473

BLAST of CSPI03G29520 vs. ExPASy TrEMBL
Match: A0A6J1J3P0 (RNA polymerase sigma factor sigD, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111481519 PE=3 SV=1)

HSP 1 Score: 723.8 bits (1867), Expect = 4.7e-205
Identity = 378/469 (80.60%), Postives = 403/469 (85.93%), Query Frame = 0

Query: 1   MATTSSLCSLPAQSPTLPSISLSSLPSLKTHLPQQPHFPFPPFSTKLGPNIISNDTLTLP 60
           MATTSSLCSLPAQSPTLPSISLSSLPSLKTHLPQQPHF   PFSTKL PN ISNDTLT P
Sbjct: 1   MATTSSLCSLPAQSPTLPSISLSSLPSLKTHLPQQPHFTISPFSTKLAPNFISNDTLTHP 60

Query: 61  PPPEAAGVAVEAAGEHVLVEAKGFSEDWSSKNVEKMEESCVAVEAAGEHVLVEAKGFSED 120
           PP EAA V                                +AVEAA EHVLVEA   S+D
Sbjct: 61  PPSEAAAV--------------------------------LAVEAAREHVLVEAGWLSKD 120

Query: 121 WSSKNVEKMEESCVGDGTVAWKKKRRKRRKEVGVKAEENWIALSSGPLRPGYLSPKEEAE 180
           W  KN+EKME+SC GDG VAWKK+RRKRRKEVG  AEENW+ALSSGPLRPG+LS KEEAE
Sbjct: 121 WRCKNLEKMEQSCGGDGNVAWKKRRRKRRKEVGETAEENWVALSSGPLRPGHLSSKEEAE 180

Query: 181 LCLCLKEAVLLENVKTRIMETQEHEPTNKQLAIAMGTKSGCIDRILCRARESRDRLIRCY 240
           LCLCLKEA  +E  KTRIMETQ+HEPTNKQLAIAMGTK G IDR+L RARESRDRLIRCY
Sbjct: 181 LCLCLKEAAQIEKTKTRIMETQDHEPTNKQLAIAMGTKLGYIDRVLWRARESRDRLIRCY 240

Query: 241 SKLVISIAAPYQGKGLSLQDLTQEGNIGLLKGAERFEPNRGHKLSTYAYWWIRQAIIRAL 300
           SKLVISIAAPYQGKG+SLQDLTQEGNIGLLKGAERFEPNRGHKLSTYAYWWIRQ II+AL
Sbjct: 241 SKLVISIAAPYQGKGISLQDLTQEGNIGLLKGAERFEPNRGHKLSTYAYWWIRQTIIQAL 300

Query: 301 DKKSRLVRLPGHMGQMVARIAEENNHLGIKLSRPPTNKEVAESLKVHLSTIRLVMERSKR 360
           DKKSRLVRLPGHMGQMVARIAE NNHLGIKLSR PT+KEVA+ LKV +ST+RLV ERSKR
Sbjct: 301 DKKSRLVRLPGHMGQMVARIAEANNHLGIKLSRVPTHKEVAKYLKVPVSTVRLVTERSKR 360

Query: 361 PISINQPVNDRSCLTLQEIMPGPDNLTPENMVMRKLMKQELKRLLNTLSKREARILSLYF 420
           PISIN+PVNDR CLTLQEIMPGPDNLTPENMV+RKLMKQELKRLL+TLSKREA IL+LYF
Sbjct: 361 PISINRPVNDRGCLTLQEIMPGPDNLTPENMVIRKLMKQELKRLLSTLSKREAHILTLYF 420

Query: 421 GLNGEIPQSFEEIGKSLNLSRETIRQTNIATLSKLKKTDVLDYLKDYLV 470
           GLNG+IPQSFEEIGKSLNLSRETIRQTNIATL+KLKK+++LDYLKDYLV
Sbjct: 421 GLNGQIPQSFEEIGKSLNLSRETIRQTNIATLAKLKKSNILDYLKDYLV 437

BLAST of CSPI03G29520 vs. ExPASy TrEMBL
Match: A0A6J1FAN3 (RNA polymerase sigma factor sigD, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111442316 PE=3 SV=1)

HSP 1 Score: 722.6 bits (1864), Expect = 1.0e-204
Identity = 376/469 (80.17%), Postives = 404/469 (86.14%), Query Frame = 0

Query: 1   MATTSSLCSLPAQSPTLPSISLSSLPSLKTHLPQQPHFPFPPFSTKLGPNIISNDTLTLP 60
           MATTSSLCSLPAQSPTLPSISLSSLPSLKTHLPQQPHF   PFSTKL PN I+NDTLT P
Sbjct: 1   MATTSSLCSLPAQSPTLPSISLSSLPSLKTHLPQQPHFTISPFSTKLAPNFINNDTLTHP 60

Query: 61  PPPEAAGVAVEAAGEHVLVEAKGFSEDWSSKNVEKMEESCVAVEAAGEHVLVEAKGFSED 120
           PP EAA V                                +AVEAA EHVLVEA   S+D
Sbjct: 61  PPSEAAAV--------------------------------LAVEAAREHVLVEAGWLSKD 120

Query: 121 WSSKNVEKMEESCVGDGTVAWKKKRRKRRKEVGVKAEENWIALSSGPLRPGYLSPKEEAE 180
           WS KN+EKME+SC G G VAWKK+RRKRRKEVG  AEENW+ALS+GPLRPG+LS KEEAE
Sbjct: 121 WSCKNLEKMEQSCGGAGNVAWKKRRRKRRKEVGETAEENWVALSNGPLRPGHLSSKEEAE 180

Query: 181 LCLCLKEAVLLENVKTRIMETQEHEPTNKQLAIAMGTKSGCIDRILCRARESRDRLIRCY 240
           LCLCLKEA  +E +KTRIMETQEHEPTNKQLAIAMGTK G IDR+L RARESRDRLIRCY
Sbjct: 181 LCLCLKEAAQIEKIKTRIMETQEHEPTNKQLAIAMGTKLGYIDRVLWRARESRDRLIRCY 240

Query: 241 SKLVISIAAPYQGKGLSLQDLTQEGNIGLLKGAERFEPNRGHKLSTYAYWWIRQAIIRAL 300
           SKLVISIAAPYQGKG+SLQDLTQEGNIGLLKGAERFEPNRGHKLSTYAYWWIRQ II+AL
Sbjct: 241 SKLVISIAAPYQGKGISLQDLTQEGNIGLLKGAERFEPNRGHKLSTYAYWWIRQTIIQAL 300

Query: 301 DKKSRLVRLPGHMGQMVARIAEENNHLGIKLSRPPTNKEVAESLKVHLSTIRLVMERSKR 360
           DKKSRLVRLPGHMGQMVA+IAE NNHLGIKLSR PT+KEVA+ LKV +ST+RLV ER+KR
Sbjct: 301 DKKSRLVRLPGHMGQMVAKIAEANNHLGIKLSRVPTHKEVAKYLKVPVSTVRLVTERNKR 360

Query: 361 PISINQPVNDRSCLTLQEIMPGPDNLTPENMVMRKLMKQELKRLLNTLSKREARILSLYF 420
           PISINQPVNDR CLTLQEIMPGPDNLTPENMV+RKLMKQELK+LLNTLSKREA IL+LYF
Sbjct: 361 PISINQPVNDRGCLTLQEIMPGPDNLTPENMVIRKLMKQELKKLLNTLSKREAHILTLYF 420

Query: 421 GLNGEIPQSFEEIGKSLNLSRETIRQTNIATLSKLKKTDVLDYLKDYLV 470
           GLNG+IPQSFEEIGKSLNLSRETIRQTNIATL+KLKK+++LDYLKDYLV
Sbjct: 421 GLNGQIPQSFEEIGKSLNLSRETIRQTNIATLAKLKKSNILDYLKDYLV 437

BLAST of CSPI03G29520 vs. NCBI nr
Match: XP_004149725.1 (RNA polymerase sigma factor sigD, chloroplastic [Cucumis sativus] >KGN58573.1 hypothetical protein Csa_000720 [Cucumis sativus])

HSP 1 Score: 833.9 bits (2153), Expect = 6.6e-238
Identity = 434/469 (92.54%), Postives = 435/469 (92.75%), Query Frame = 0

Query: 1   MATTSSLCSLPAQSPTLPSISLSSLPSLKTHLPQQPHFPFPPFSTKLGPNIISNDTLTLP 60
           MATTSSLCSLPAQSPTLPSISLSSLPSLKTHLPQQPHFPFPPFSTKLGPNIISNDTLTLP
Sbjct: 1   MATTSSLCSLPAQSPTLPSISLSSLPSLKTHLPQQPHFPFPPFSTKLGPNIISNDTLTLP 60

Query: 61  PPPEAAGVAVEAAGEHVLVEAKGFSEDWSSKNVEKMEESCVAVEAAGEHVLVEAKGFSED 120
           PPPEAA                                  +AVEAAGEHVLVEAKGFSED
Sbjct: 61  PPPEAA---------------------------------VLAVEAAGEHVLVEAKGFSED 120

Query: 121 WSSKNVEKMEESCVGDGTVAWKKKRRKRRKEVGVKAEENWIALSSGPLRPGYLSPKEEAE 180
           WSSKNVEKMEESCVGDGTVAWKKKRRKRRKEVGVKAEENWIALSSGPLRPGYLSPKEEAE
Sbjct: 121 WSSKNVEKMEESCVGDGTVAWKKKRRKRRKEVGVKAEENWIALSSGPLRPGYLSPKEEAE 180

Query: 181 LCLCLKEAVLLENVKTRIMETQEHEPTNKQLAIAMGTKSGCIDRILCRARESRDRLIRCY 240
           LCLCLKEAVLLENVKTRIMETQEHEPTNKQLAIAMGTKSGCIDRILCRARESRDRLIRCY
Sbjct: 181 LCLCLKEAVLLENVKTRIMETQEHEPTNKQLAIAMGTKSGCIDRILCRARESRDRLIRCY 240

Query: 241 SKLVISIAAPYQGKGLSLQDLTQEGNIGLLKGAERFEPNRGHKLSTYAYWWIRQAIIRAL 300
           SKLVISIAAPYQGKGLSLQDLTQEGNIGLLKGAERFEPNRGHKLSTYAYWWIRQAIIRAL
Sbjct: 241 SKLVISIAAPYQGKGLSLQDLTQEGNIGLLKGAERFEPNRGHKLSTYAYWWIRQAIIRAL 300

Query: 301 DKKSRLVRLPGHMGQMVARIAEENNHLGIKLSRPPTNKEVAESLKVHLSTIRLVMERSKR 360
           DKKSRLVRLPGHMGQMVARIAEENNHLGIKLSRPPTNKEVAESLKVHLSTIRLVMERSKR
Sbjct: 301 DKKSRLVRLPGHMGQMVARIAEENNHLGIKLSRPPTNKEVAESLKVHLSTIRLVMERSKR 360

Query: 361 PISINQPVNDRSCLTLQEIMPGPDNLTPENMVMRKLMKQELKRLLNTLSKREARILSLYF 420
           PISINQPVNDRSCLTLQEIMPGPDNLTPENMVMRKLMKQELKRLLNTLSKREARILSLYF
Sbjct: 361 PISINQPVNDRSCLTLQEIMPGPDNLTPENMVMRKLMKQELKRLLNTLSKREARILSLYF 420

Query: 421 GLNGEIPQSFEEIGKSLNLSRETIRQTNIATLSKLKKTDVLDYLKDYLV 470
           GLNGEIPQSFEEIGKSLNLSRETIRQTNIATLSKLKKTDVLDYLKDYLV
Sbjct: 421 GLNGEIPQSFEEIGKSLNLSRETIRQTNIATLSKLKKTDVLDYLKDYLV 436

BLAST of CSPI03G29520 vs. NCBI nr
Match: KAA0048391.1 (RNA polymerase sigma factor sigD [Cucumis melo var. makuwa] >TYJ99868.1 RNA polymerase sigma factor sigD [Cucumis melo var. makuwa])

HSP 1 Score: 801.6 bits (2069), Expect = 3.6e-228
Identity = 415/469 (88.49%), Postives = 428/469 (91.26%), Query Frame = 0

Query: 1   MATTSSLCSLPAQSPTLPSISLSSLPSLKTHLPQQPHFPFPPFSTKLGPNIISNDTLTLP 60
           MATTSSLCSLPAQSPTLPSISLSSLPSLKTHLPQQPHFPFPPFS+KLGPN IS DTLTLP
Sbjct: 1   MATTSSLCSLPAQSPTLPSISLSSLPSLKTHLPQQPHFPFPPFSSKLGPNFISYDTLTLP 60

Query: 61  PPPEAAGVAVEAAGEHVLVEAKGFSEDWSSKNVEKMEESCVAVEAAGEHVLVEAKGFSED 120
           PPPEAA                                  +AVEAAGEHVLVEA+GFSED
Sbjct: 61  PPPEAA---------------------------------VLAVEAAGEHVLVEARGFSED 120

Query: 121 WSSKNVEKMEESCVGDGTVAWKKKRRKRRKEVGVKAEENWIALSSGPLRPGYLSPKEEAE 180
           WSSKNVE+MEESCVGDGTVAWKKKRRKRRKEVGVKAEENWIALSSGPLRPGYLSPKEEAE
Sbjct: 121 WSSKNVERMEESCVGDGTVAWKKKRRKRRKEVGVKAEENWIALSSGPLRPGYLSPKEEAE 180

Query: 181 LCLCLKEAVLLENVKTRIMETQEHEPTNKQLAIAMGTKSGCIDRILCRARESRDRLIRCY 240
           LCLCLKEAVLLE VK+RIMETQEHEPTNKQLAIAMGTK GCIDRILCRARESRDRLIRCY
Sbjct: 181 LCLCLKEAVLLEKVKSRIMETQEHEPTNKQLAIAMGTKPGCIDRILCRARESRDRLIRCY 240

Query: 241 SKLVISIAAPYQGKGLSLQDLTQEGNIGLLKGAERFEPNRGHKLSTYAYWWIRQAIIRAL 300
           SKLVISIAAPYQGKGLSLQDLTQEGNIGLLKGAERFEP+RGHKLSTYAYWWIRQAIIRAL
Sbjct: 241 SKLVISIAAPYQGKGLSLQDLTQEGNIGLLKGAERFEPSRGHKLSTYAYWWIRQAIIRAL 300

Query: 301 DKKSRLVRLPGHMGQMVARIAEENNHLGIKLSRPPTNKEVAESLKVHLSTIRLVMERSKR 360
           DKKSRLVRLPGHMGQMVARIAEENNHLGIKLSRPPTN+EVAESLKV +ST+RLVMERSKR
Sbjct: 301 DKKSRLVRLPGHMGQMVARIAEENNHLGIKLSRPPTNEEVAESLKVRVSTVRLVMERSKR 360

Query: 361 PISINQPVNDRSCLTLQEIMPGPDNLTPENMVMRKLMKQELKRLLNTLSKREARILSLYF 420
           PISINQPVNDRSCLTLQEI+PGPDNLTPENMVMRKLMKQELKRLLNTLSKREA ILSLYF
Sbjct: 361 PISINQPVNDRSCLTLQEIIPGPDNLTPENMVMRKLMKQELKRLLNTLSKREAHILSLYF 420

Query: 421 GLNGEIPQSFEEIGKSLNLSRETIRQTNIATLSKLKKTDVLDYLKDYLV 470
           GLNGEIPQSFEEIGKSLNLSRETIR+ NIATLSKLKKT+VLDY+KDYLV
Sbjct: 421 GLNGEIPQSFEEIGKSLNLSRETIRRINIATLSKLKKTNVLDYIKDYLV 436

BLAST of CSPI03G29520 vs. NCBI nr
Match: XP_008461821.1 (PREDICTED: RNA polymerase sigma factor sigD, chloroplastic [Cucumis melo])

HSP 1 Score: 779.6 bits (2012), Expect = 1.5e-221
Identity = 402/456 (88.16%), Postives = 415/456 (91.01%), Query Frame = 0

Query: 14  SPTLPSISLSSLPSLKTHLPQQPHFPFPPFSTKLGPNIISNDTLTLPPPPEAAGVAVEAA 73
           SPTLPSISLSSLPSLKTHLPQQPHFPFPPFS+KLGPN IS DTLTLPPPPEAA       
Sbjct: 51  SPTLPSISLSSLPSLKTHLPQQPHFPFPPFSSKLGPNFISYDTLTLPPPPEAA------- 110

Query: 74  GEHVLVEAKGFSEDWSSKNVEKMEESCVAVEAAGEHVLVEAKGFSEDWSSKNVEKMEESC 133
                                      +AVEAAGEHVLVEA+GFSEDWSSKNVE+MEESC
Sbjct: 111 --------------------------VLAVEAAGEHVLVEARGFSEDWSSKNVERMEESC 170

Query: 134 VGDGTVAWKKKRRKRRKEVGVKAEENWIALSSGPLRPGYLSPKEEAELCLCLKEAVLLEN 193
           VGDGTVAWKKKRRKRRKEVGVKAEENWIALSSGPLRPGYLSPKEEAELCLCLKEAVLLE 
Sbjct: 171 VGDGTVAWKKKRRKRRKEVGVKAEENWIALSSGPLRPGYLSPKEEAELCLCLKEAVLLEK 230

Query: 194 VKTRIMETQEHEPTNKQLAIAMGTKSGCIDRILCRARESRDRLIRCYSKLVISIAAPYQG 253
           VK+RIMETQEHEPTNKQLAIAMGTK GCIDRILCRARESRDRLIRCYSKLVISIAAPYQG
Sbjct: 231 VKSRIMETQEHEPTNKQLAIAMGTKPGCIDRILCRARESRDRLIRCYSKLVISIAAPYQG 290

Query: 254 KGLSLQDLTQEGNIGLLKGAERFEPNRGHKLSTYAYWWIRQAIIRALDKKSRLVRLPGHM 313
           KGLSLQDLTQEGNIGLLKGAERFEP+RGHKLSTYAYWWIRQAIIRALDKKSRLVRLPGHM
Sbjct: 291 KGLSLQDLTQEGNIGLLKGAERFEPSRGHKLSTYAYWWIRQAIIRALDKKSRLVRLPGHM 350

Query: 314 GQMVARIAEENNHLGIKLSRPPTNKEVAESLKVHLSTIRLVMERSKRPISINQPVNDRSC 373
           GQMVARIAEENNHLGIKLSRPPTN+EVAESLKV +ST+RLVMERSKRPISINQPVNDRSC
Sbjct: 351 GQMVARIAEENNHLGIKLSRPPTNEEVAESLKVRVSTVRLVMERSKRPISINQPVNDRSC 410

Query: 374 LTLQEIMPGPDNLTPENMVMRKLMKQELKRLLNTLSKREARILSLYFGLNGEIPQSFEEI 433
           LTLQEI+PGPDNLTPENMVMRKLMKQELKRLLNTLSKREA ILSLYFGLNGEIPQSFEEI
Sbjct: 411 LTLQEIIPGPDNLTPENMVMRKLMKQELKRLLNTLSKREAHILSLYFGLNGEIPQSFEEI 470

Query: 434 GKSLNLSRETIRQTNIATLSKLKKTDVLDYLKDYLV 470
           GKSLNLSRETIR+ NIATLSKLKKT+VLDY+KDYLV
Sbjct: 471 GKSLNLSRETIRRINIATLSKLKKTNVLDYIKDYLV 473

BLAST of CSPI03G29520 vs. NCBI nr
Match: XP_038906215.1 (RNA polymerase sigma factor sigD, chloroplastic [Benincasa hispida])

HSP 1 Score: 766.1 bits (1977), Expect = 1.7e-217
Identity = 395/469 (84.22%), Postives = 413/469 (88.06%), Query Frame = 0

Query: 1   MATTSSLCSLPAQSPTLPSISLSSLPSLKTHLPQQPHFPFPPFSTKLGPNIISNDTLTLP 60
           MATTSSLCSLP QSPTLPSISLSSLPSLKTHLPQQPHFPFPPFSTKLG N I+NDTLTLP
Sbjct: 1   MATTSSLCSLPTQSPTLPSISLSSLPSLKTHLPQQPHFPFPPFSTKLGTNFINNDTLTLP 60

Query: 61  PPPEAAGVAVEAAGEHVLVEAKGFSEDWSSKNVEKMEESCVAVEAAGEHVLVEAKGFSED 120
           PPPEAA                                  + VEAA EHVLV+A+GFSED
Sbjct: 61  PPPEAA---------------------------------VLTVEAAREHVLVDARGFSED 120

Query: 121 WSSKNVEKMEESCVGDGTVAWKKKRRKRRKEVGVKAEENWIALSSGPLRPGYLSPKEEAE 180
           WS KN+E+ME SC GDGTVAWKKKRRKRRKEVG+KAEENW ALSSGPLRPGYLSPKEEAE
Sbjct: 121 WSCKNLERMEGSCGGDGTVAWKKKRRKRRKEVGLKAEENWAALSSGPLRPGYLSPKEEAE 180

Query: 181 LCLCLKEAVLLENVKTRIMETQEHEPTNKQLAIAMGTKSGCIDRILCRARESRDRLIRCY 240
           LCLCLKEA  LE  KTRIMETQEHEPTN+QLAIA+GTK G IDRILCRARESRDRLIRCY
Sbjct: 181 LCLCLKEAAQLEEAKTRIMETQEHEPTNRQLAIALGTKLGSIDRILCRARESRDRLIRCY 240

Query: 241 SKLVISIAAPYQGKGLSLQDLTQEGNIGLLKGAERFEPNRGHKLSTYAYWWIRQAIIRAL 300
           SKLVISIAAPYQGKGLSLQDLTQEGNIGLLKGAERFEPNRGHKLSTYAYWWIRQAIIRAL
Sbjct: 241 SKLVISIAAPYQGKGLSLQDLTQEGNIGLLKGAERFEPNRGHKLSTYAYWWIRQAIIRAL 300

Query: 301 DKKSRLVRLPGHMGQMVARIAEENNHLGIKLSRPPTNKEVAESLKVHLSTIRLVMERSKR 360
           DKKSRLVRLPGHMGQMVARIAE NNHLG+KLSRPPT+KEVAESLKV +ST+RLV ERSKR
Sbjct: 301 DKKSRLVRLPGHMGQMVARIAEANNHLGLKLSRPPTHKEVAESLKVRVSTVRLVTERSKR 360

Query: 361 PISINQPVNDRSCLTLQEIMPGPDNLTPENMVMRKLMKQELKRLLNTLSKREARILSLYF 420
           PISINQPVNDR CLTLQEIMPGPDNLTPENMVMRKLMKQE+KRLLNTLSKREA ILSLYF
Sbjct: 361 PISINQPVNDRGCLTLQEIMPGPDNLTPENMVMRKLMKQEVKRLLNTLSKREAHILSLYF 420

Query: 421 GLNGEIPQSFEEIGKSLNLSRETIRQTNIATLSKLKKTDVLDYLKDYLV 470
           GLNGEIP+SFEEIGKSLNLSRETIRQ N+ATLSKLK TD+LDYLK+YLV
Sbjct: 421 GLNGEIPRSFEEIGKSLNLSRETIRQINVATLSKLKNTDILDYLKEYLV 436

BLAST of CSPI03G29520 vs. NCBI nr
Match: XP_023528248.1 (RNA polymerase sigma factor sigD, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 724.9 bits (1870), Expect = 4.3e-205
Identity = 377/469 (80.38%), Postives = 404/469 (86.14%), Query Frame = 0

Query: 1   MATTSSLCSLPAQSPTLPSISLSSLPSLKTHLPQQPHFPFPPFSTKLGPNIISNDTLTLP 60
           MATTSSLCSLPAQSPTLPSISLSSLPSLKTHLPQQPHF   PFSTKL PN I+NDTLT P
Sbjct: 1   MATTSSLCSLPAQSPTLPSISLSSLPSLKTHLPQQPHFTISPFSTKLAPNFINNDTLTHP 60

Query: 61  PPPEAAGVAVEAAGEHVLVEAKGFSEDWSSKNVEKMEESCVAVEAAGEHVLVEAKGFSED 120
           PP EAA V                                +AVEAA EHVLVEA   S+D
Sbjct: 61  PPSEAAAV--------------------------------LAVEAAREHVLVEAGWLSKD 120

Query: 121 WSSKNVEKMEESCVGDGTVAWKKKRRKRRKEVGVKAEENWIALSSGPLRPGYLSPKEEAE 180
           W  KN+EKME+SC GDG VAWKK+RRKRRKEVG  AEENW+ALS+GPLRPG+LS KEEAE
Sbjct: 121 WRCKNLEKMEQSCGGDGNVAWKKRRRKRRKEVGETAEENWVALSNGPLRPGHLSSKEEAE 180

Query: 181 LCLCLKEAVLLENVKTRIMETQEHEPTNKQLAIAMGTKSGCIDRILCRARESRDRLIRCY 240
           LCLCLKEA  +E +KTRIMETQ+HEPTNKQLAIAMGTK G IDR+L RARESRDRLIRCY
Sbjct: 181 LCLCLKEAAQIEKIKTRIMETQDHEPTNKQLAIAMGTKLGYIDRVLWRARESRDRLIRCY 240

Query: 241 SKLVISIAAPYQGKGLSLQDLTQEGNIGLLKGAERFEPNRGHKLSTYAYWWIRQAIIRAL 300
           SKLVISIAAPYQGKG+SLQDLTQEGNIGLLKGAERFEPNRGHKLSTYAYWWIRQ II+AL
Sbjct: 241 SKLVISIAAPYQGKGISLQDLTQEGNIGLLKGAERFEPNRGHKLSTYAYWWIRQTIIQAL 300

Query: 301 DKKSRLVRLPGHMGQMVARIAEENNHLGIKLSRPPTNKEVAESLKVHLSTIRLVMERSKR 360
           DKKSRLVRLPGHMGQMVARIAE NNHLGIKLSR PT+KEVA+ LKV +ST+RLV ERSKR
Sbjct: 301 DKKSRLVRLPGHMGQMVARIAEANNHLGIKLSRVPTHKEVAKYLKVPVSTVRLVTERSKR 360

Query: 361 PISINQPVNDRSCLTLQEIMPGPDNLTPENMVMRKLMKQELKRLLNTLSKREARILSLYF 420
           PISINQPVNDR CLTLQEIMPGPDNLTPENMV+RKLMKQELK+LLNTLSKREA IL+LYF
Sbjct: 361 PISINQPVNDRGCLTLQEIMPGPDNLTPENMVIRKLMKQELKKLLNTLSKREAHILTLYF 420

Query: 421 GLNGEIPQSFEEIGKSLNLSRETIRQTNIATLSKLKKTDVLDYLKDYLV 470
           GLNG+IPQSFEEIGKSLNLSRETIRQTNIATL+KLKK+++LDYLKDYLV
Sbjct: 421 GLNGQIPQSFEEIGKSLNLSRETIRQTNIATLAKLKKSNILDYLKDYLV 437

BLAST of CSPI03G29520 vs. TAIR 10
Match: AT5G13730.1 (sigma factor 4 )

HSP 1 Score: 321.2 bits (822), Expect = 1.3e-87
Identity = 171/345 (49.57%), Postives = 238/345 (68.99%), Query Frame = 0

Query: 137 GTVAWKKKRRKRRKEV-------------GVKAEENWIALS-SGPLRPGYLSPKEEAELC 196
           G  + K+++R+RR+ V             GV+AE   I++   G  R G+LS  EE +LC
Sbjct: 73  GIQSEKRRKRRRRRRVGYERLEPEEEENAGVEAEAETISVPVVGASRSGFLSRLEEVQLC 132

Query: 197 LCLKEAVLLENVKTRIMETQEHEPTNKQLAIAMGTKSGCIDRILCRARESRDRLIRCYSK 256
           L LKE   LEN+ T +   +E+E  +  LA   G K    + ILCR +E+R+++ RCY +
Sbjct: 133 LYLKEGAKLENLGTSV---EENEMVSVLLASGRGKKKRSANEILCRRKEAREKITRCYRR 192

Query: 257 LVISIAAPYQGKGLSLQDLTQEGNIGLLKGAERFEPNRGHKLSTYAYWWIRQAIIRALDK 316
           LV+SIA  YQGKGL+LQDL QEG+IGLL+GAERF+P+RG+KLSTY YWWI+QAI+RA+  
Sbjct: 193 LVVSIATGYQGKGLNLQDLIQEGSIGLLRGAERFDPDRGYKLSTYVYWWIKQAILRAIAH 252

Query: 317 KSRLVRLPGHMGQMVARIAEENNHLGIKLSRPPTNKEVAESLKVHLSTIRLVMERSKRPI 376
           KSRLV+LPG M ++ A++AE +N L  KL R P+ +E+AE L +++S +RL +ERS+ P+
Sbjct: 253 KSRLVKLPGSMWELTAKVAEASNVLTRKLRRQPSCEEIAEHLNLNVSAVRLAVERSRSPV 312

Query: 377 SINQPVNDRSCLTLQEIMPGPDNLTPENMVMRKLMKQELKRLLNTLSKREARILSLYFGL 436
           S+++  +    +TLQEI+ GPD   PE MV R+ MK E+++LL +L+ RE+R+L LYFGL
Sbjct: 313 SLDRVASQNGRMTLQEIVRGPDETRPEEMVKREHMKHEIEQLLGSLTARESRVLGLYFGL 372

Query: 437 NGEIPQSFEEIGKSLNLSRETIRQTNIATLSKLKKTDVLDYLKDY 468
           NGE P SFEEIGKSL LSRE +RQ N   L KL+    ++ LK Y
Sbjct: 373 NGETPMSFEEIGKSLKLSRERVRQINGIALKKLRNVHNVNDLKIY 414

BLAST of CSPI03G29520 vs. TAIR 10
Match: AT1G08540.1 (RNApolymerase sigma subunit 2 )

HSP 1 Score: 194.9 bits (494), Expect = 1.5e-49
Identity = 120/390 (30.77%), Postives = 212/390 (54.36%), Query Frame = 0

Query: 80  EAKGFSEDWSSKNVEKMEESCVAVEAAGEHVLVEAKGFSEDWSSKNVEKMEESCVGDGTV 139
           E+ G  E    + VE +EE      A       E K       +K +EK         T 
Sbjct: 186 ESIGDLESEKQEEVELLEEQPSVSLAVRSTRQTERKA----RRAKGLEKTASGIPSVKTG 245

Query: 140 AWKKKRRKRRKEVGVKAEENWIALSSGPLRPGYLSPKEEAELCLCLKEAVLLENVKTRIM 199
           +  KK+R   +EV       ++ +++   +   L+ +EE EL   +++ + LE ++T + 
Sbjct: 246 SSPKKKRLVAQEVDHNDPLRYLRMTTSSSK--LLTVREEHELSAGIQDLLKLERLQTELT 305

Query: 200 ETQEHEPTNKQLAIAMGTKSGCIDRILCRARESRDRLIRCYSKLVISIAAPYQGKGLSLQ 259
           E    +PT  Q A A G     + + +      +D++I+   +LVISIA  YQG G++LQ
Sbjct: 306 ERSGRQPTFAQWASAAGVDQKSLRQRIHHGTLCKDKMIKSNIRLVISIAKNYQGAGMNLQ 365

Query: 260 DLTQEGNIGLLKGAERFEPNRGHKLSTYAYWWIRQAIIRALDKKSRLVRLPGHMGQMVAR 319
           DL QEG  GL++GAE+F+  +G K STYA+WWI+QA+ ++L  +SR++RLP HM +   R
Sbjct: 366 DLVQEGCRGLVRGAEKFDATKGFKFSTYAHWWIKQAVRKSLSDQSRMIRLPFHMVEATYR 425

Query: 320 IAEENNHLGIKLSRPPTNKEVAESLKVHLSTIRLVMERSKRPISINQPVNDRSCLTLQEI 379
           + E    L  +  + P N+E+AE+  + +  +  V+   K P S++Q +     L   E+
Sbjct: 426 VKEARKQLYSETGKHPKNEEIAEATGLSMKRLMAVLLSPKPPRSLDQKIGMNQNLKPSEV 485

Query: 380 MPGPDNLTPENMVMRKLMKQELKRLLNTLSKREARILSLYFGLNGEIPQSFEEIGKSLNL 439
           +  P+ +T E++++++ M+Q+L ++L++L  RE +++   FG+     ++ +EIG+ + +
Sbjct: 486 IADPEAVTSEDILIKEFMRQDLDKVLDSLGTREKQVIRWRFGMEDGRMKTLQEIGEMMGV 545

Query: 440 SRETIRQTNIATLSKLKKTDVLDYLKDYLV 470
           SRE +RQ   +   KLK     ++L+ YLV
Sbjct: 546 SRERVRQIESSAFRKLKNKKRNNHLQQYLV 569

BLAST of CSPI03G29520 vs. TAIR 10
Match: AT2G36990.1 (RNApolymerase sigma-subunit F )

HSP 1 Score: 194.5 bits (493), Expect = 1.9e-49
Identity = 114/335 (34.03%), Postives = 187/335 (55.82%), Query Frame = 0

Query: 123 SKNVEKMEESCVGDGTVAWKKKRRKRRKEVGVKAEENWIALSSGPLRPGYLSPKEEAELC 182
           +KN    + + V D     +K   K++ + G   ++       GP     L+ KEEAEL 
Sbjct: 198 AKNRRAPKSNDVDDEGYVPQKTSAKKKYKQGADNDDALQLFLWGPETKQLLTAKEEAELI 257

Query: 183 LCLKEAVLLENVKTRIMETQEHEPTNKQLAIAMGTKSGCIDRILCRARESRDRLIRCYSK 242
             ++  + LE VKT++      EPT  + A AMG  S  +   + R R SR++LI    +
Sbjct: 258 SHIQHLLKLEKVKTKLESQNGCEPTIGEWAEAMGISSPVLKSDIHRGRSSREKLITANLR 317

Query: 243 LVISIAAPYQGKGLSLQDLTQEGNIGLLKGAERFEPNRGHKLSTYAYWWIRQAIIRALDK 302
           LV+ IA  YQ +GL+ QDL QEG++GL+K  E+F+P  G + +TYAYWWIRQ+I +++ +
Sbjct: 318 LVVHIAKQYQNRGLNFQDLLQEGSMGLMKSVEKFKPQSGCRFATYAYWWIRQSIRKSIFQ 377

Query: 303 KSRLVRLPGHMGQMVARIAEENNHLGIKLSRPPTNKEVAESLKVHLSTIRLVMERSKRPI 362
            SR +RLP ++  ++ +++E       + +  P+ +E+A  + V    +  ++  ++ P+
Sbjct: 378 NSRTIRLPENVYMLLGKVSEARKTCVQEGNYRPSKEELAGHVGVSTEKLDKLLYNTRTPL 437

Query: 363 SINQPVNDRSCLTLQEIMPGPDNLTPENMVMRKLMKQELKRLLNTLSKREARILSLYFGL 422
           S+ QP+      T QEI P     TP   V ++LM+  ++ LLN LS +E RI+ L FG+
Sbjct: 438 SMQQPIWSDQDTTFQEITPDSGIETPTMSVGKQLMRNHVRNLLNVLSPKERRIIKLRFGI 497

Query: 423 NGEIPQSFEEIGKSLNLSRETIRQTNIATLSKLKK 458
           +G   +S  EIG+   LS+E +RQ     L +LK+
Sbjct: 498 DGGKQRSLSEIGEIYGLSKERVRQLESRALYRLKQ 532

BLAST of CSPI03G29520 vs. TAIR 10
Match: AT1G64860.1 (sigma factor A )

HSP 1 Score: 146.4 bits (368), Expect = 5.9e-35
Identity = 118/417 (28.30%), Postives = 195/417 (46.76%), Query Frame = 0

Query: 79  VEAKGFSEDWSSKNVEK-MEESCVAVEAAGEH-----VLVEAKGFSEDWSSKNVEKMEES 138
           V+     + W     +K +EE C   +    H     +L++     + W+    + +   
Sbjct: 78  VDVASTEKPWLPNGTDKELEEECYDDDDLISHSVEAILLLQKSMLEKSWNLSFEKAVSSE 137

Query: 139 CVGDGTVAWKK-------KRRKRRKEVGVKAEENWI------ALSSGPLRPGY------- 198
             G GT+  KK           R++ +G K + N         +SSG    GY       
Sbjct: 138 YPGKGTIRKKKIPVITCSGISARQRRIGAKKKTNMTHVKAVSDVSSGKQVRGYVKGVISE 197

Query: 199 --LSPKEEAELCLCLKEAVLLENVKTRIMETQEHEPTNKQLAIAMGTKSGCIDRILCRAR 258
             LS  E   L   +K  + L++ K+R+ +    EP+++QLA+++      +   L    
Sbjct: 198 DVLSHVEVVRLSKKIKSGLRLDDHKSRLKDRLGCEPSDEQLAVSLKISRAELQAWLMECH 257

Query: 259 ESRDRLIRCYSKLVISIAAPYQGKGLSLQDLTQEGNIGLLKGAERFEPNRGHKLSTYAYW 318
            +R++L     +LV+SIA  Y   G  + DL Q G IGLL+G E+F+ ++G ++STY YW
Sbjct: 258 LAREKLAMSNVRLVMSIAQRYDNLGAEMSDLVQGGLIGLLRGIEKFDSSKGFRISTYVYW 317

Query: 319 WIRQAIIRALDKKSRLVRLPGHMGQMVARIAEENNHLGIKLSRPPTNKEVAESLKVHLST 378
           WIRQ + RAL   SR +RLP H+ + +  I      L  K   P  ++ +AESL +    
Sbjct: 318 WIRQGVSRALVDNSRTLRLPTHLHERLGLIRNAKLRLQEKGITPSIDR-IAESLNMSQKK 377

Query: 379 IRLVMERSKRPISINQPVNDRSCLTLQEIMPGPDNLT----------PENMVMRKLMKQE 438
           +R   E   +  S+     DR        +PG  + +          P +      +K+E
Sbjct: 378 VRNATEAVSKVFSL-----DRDAFPSLNGLPGETHHSYIADTRLENNPWHGYDDLALKEE 437

Query: 439 LKRLLN-TLSKREARILSLYFGLNGEIPQSFEEIGKSLNLSRETIRQTNIATLSKLK 457
           + +L++ TL +RE  I+ LY+GL+ E   ++E+I K + LSRE +RQ  +  L KLK
Sbjct: 438 VSKLISATLGEREKEIIRLYYGLDKEC-LTWEDISKRIGLSRERVRQVGLVALEKLK 487

BLAST of CSPI03G29520 vs. TAIR 10
Match: AT5G24120.1 (sigma factor E )

HSP 1 Score: 135.6 bits (340), Expect = 1.0e-31
Identity = 93/306 (30.39%), Postives = 157/306 (51.31%), Query Frame = 0

Query: 170 PGYLSPKEEAELCLCLKEAVLLENVKTRIMETQEHEPTNKQLAIAMGTKSGCIDRILCRA 229
           P  LS  E A L   ++    L  VK  + ++   EP   ++A  +   +G + + +   
Sbjct: 217 PPVLSSTEHAWLFKLMQPMKALLEVKDVLQKSLGREPREAEIAGEINMTAGEVKKKIEIG 276

Query: 230 RESRDRLIRCYSKLVISIAAPYQGK---GLSLQDLTQEGNIGLLKGAERFEPNRGHKLST 289
           R +R++LI+   +LV+ +   Y  +   G   QDL Q G  GL+   +RFEP R  +LST
Sbjct: 277 RAARNKLIKHNLRLVLFVMNKYFHEFTNGPKFQDLCQAGMRGLITAIDRFEPKRKFRLST 336

Query: 290 YAYWWIRQAIIRALDKKSRLVRLPGHMGQMVARIAEENNHLGIKLSRPPTNKEVAESLKV 349
           Y  +WIR AIIR++   S   R+P  +  +   I +    L  ++ RPPT  EV E LK+
Sbjct: 337 YGLFWIRHAIIRSM-TTSNFTRVPFGLESVRVEIYKAKTELWFEMGRPPTEDEVVERLKI 396

Query: 350 HLSTIRLVMERSKRPISINQPVNDRSCLTLQEIMPG---PDNLTPENMVMRKLMKQELKR 409
                R V+  +K  +S+    N +  +T +E + G    D +   N     L++  L  
Sbjct: 397 TPERYREVLRAAKPVLSL----NSKHSVTQEEFINGITDVDGVGANNSRQPALLRLALDD 456

Query: 410 LLNTLSKREARILSLYFGLNGEIPQSFEEIGKSLNLSRETIRQTNIATLSKLKKTDVLDY 469
           +L++L  +E+ ++   +GL+G+  ++  EI  +LN+SRE +R+  +  L KLK    +DY
Sbjct: 457 VLDSLKPKESLVIRQRYGLDGKGDRTLGEIAGNLNISREMVRKHEVKALMKLKHQARVDY 516

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9ZSL61.9e-8649.57RNA polymerase sigma factor sigD, chloroplastic OS=Arabidopsis thaliana OX=3702 ... [more]
P380236.4e-5034.12RNA polymerase sigma factor SigA1 OS=Synechococcus elongatus (strain PCC 7942 / ... [more]
P266839.2e-4932.74RNA polymerase sigma factor SigA OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UT... [more]
O220562.0e-4830.77RNA polymerase sigma factor sigB OS=Arabidopsis thaliana OX=3702 GN=SIGB PE=2 SV... [more]
Q9LD952.7e-4834.03RNA polymerase sigma factor sigF, chloroplastic OS=Arabidopsis thaliana OX=3702 ... [more]
Match NameE-valueIdentityDescription
A0A0A0L9T03.2e-23892.54Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G687740 PE=3 SV=1[more]
A0A5D3BP271.8e-22888.49RNA polymerase sigma factor sigD OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A1S3CFL77.2e-22288.16RNA polymerase sigma factor sigD, chloroplastic OS=Cucumis melo OX=3656 GN=LOC10... [more]
A0A6J1J3P04.7e-20580.60RNA polymerase sigma factor sigD, chloroplastic OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1FAN31.0e-20480.17RNA polymerase sigma factor sigD, chloroplastic OS=Cucurbita moschata OX=3662 GN... [more]
Match NameE-valueIdentityDescription
XP_004149725.16.6e-23892.54RNA polymerase sigma factor sigD, chloroplastic [Cucumis sativus] >KGN58573.1 hy... [more]
KAA0048391.13.6e-22888.49RNA polymerase sigma factor sigD [Cucumis melo var. makuwa] >TYJ99868.1 RNA poly... [more]
XP_008461821.11.5e-22188.16PREDICTED: RNA polymerase sigma factor sigD, chloroplastic [Cucumis melo][more]
XP_038906215.11.7e-21784.22RNA polymerase sigma factor sigD, chloroplastic [Benincasa hispida][more]
XP_023528248.14.3e-20580.38RNA polymerase sigma factor sigD, chloroplastic [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT5G13730.11.3e-8749.57sigma factor 4 [more]
AT1G08540.11.5e-4930.77RNApolymerase sigma subunit 2 [more]
AT2G36990.11.9e-4934.03RNApolymerase sigma-subunit F [more]
AT1G64860.15.9e-3528.30sigma factor A [more]
AT5G24120.11.0e-3130.39sigma factor E [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 394..414
NoneNo IPR availableGENE3D1.10.601.10RNA Polymerase Primary Sigma Factorcoord: 188..308
e-value: 3.1E-35
score: 124.3
NoneNo IPR availablePANTHERPTHR30603:SF47RNA POLYMERASE SIGMA FACTOR SIGD, CHLOROPLASTICcoord: 4..468
NoneNo IPR availablePANTHERPTHR30603RNA POLYMERASE SIGMA FACTOR RPOcoord: 4..468
NoneNo IPR availableCDDcd06171Sigma70_r4coord: 398..456
e-value: 2.24287E-7
score: 45.5582
IPR000943RNA polymerase sigma-70PRINTSPR00046SIGMA70FCTcoord: 408..420
score: 37.87
coord: 444..455
score: 41.29
coord: 284..292
score: 74.91
coord: 429..444
score: 39.64
coord: 260..273
score: 58.71
IPR007630RNA polymerase sigma-70 region 4PFAMPF04545Sigma70_r4coord: 404..457
e-value: 3.6E-11
score: 42.4
IPR007627RNA polymerase sigma-70 region 2PFAMPF04542Sigma70_r2coord: 236..305
e-value: 2.1E-20
score: 72.2
IPR036388Winged helix-like DNA-binding domain superfamilyGENE3D1.10.10.10coord: 309..384
e-value: 1.7E-8
score: 36.7
IPR036388Winged helix-like DNA-binding domain superfamilyGENE3D1.10.10.10coord: 387..465
e-value: 2.5E-19
score: 70.9
IPR014284RNA polymerase sigma-70 like domainTIGRFAMTIGR02937TIGR02937coord: 232..457
e-value: 6.5E-31
score: 105.2
IPR007624RNA polymerase sigma-70 region 3PFAMPF04539Sigma70_r3coord: 319..391
e-value: 1.6E-10
score: 41.0
IPR013324RNA polymerase sigma factor, region 3/4-likeSUPERFAMILY88659Sigma3 and sigma4 domains of RNA polymerase sigma factorscoord: 309..380
IPR013324RNA polymerase sigma factor, region 3/4-likeSUPERFAMILY88659Sigma3 and sigma4 domains of RNA polymerase sigma factorscoord: 367..458
IPR013325RNA polymerase sigma factor, region 2SUPERFAMILY88946Sigma2 domain of RNA polymerase sigma factorscoord: 170..305

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI03G29520.1CSPI03G29520.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071482 cellular response to light stimulus
biological_process GO:2000142 regulation of DNA-templated transcription, initiation
biological_process GO:0006352 DNA-templated transcription, initiation
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0003677 DNA binding
molecular_function GO:0016987 sigma factor activity
molecular_function GO:0003700 DNA-binding transcription factor activity