CSPI03G29370 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI03G29370
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Hydroxymethylbilane synthase
Location	Chr3: 26943472 .. 26946664 (-)
RNA-Seq Expression	CSPI03G29370
Synteny	CSPI03G29370

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GAAATGGATGAGAATCCTATCCGCCAACGCAAATGCAGACATTTGCCCTGTTGGGTCATCAAAAACAATCCCTTCTAAGCTAACCCATTTCCCCTTTTTGCTTTCTTTCTTCTTCTCCTCCTCCTCCTCCTCCTCCTCATCTCTCTCAATCTCAAACAAACACTCTCTCAAATGGGTGTTTTATCTTCCCCTTCTGTAAGCCACTCTCCAATGCCTCGCCCTTGTAATGTTGGTTCTCTTTCTCTTCTTGGGTTCTCTTCACTTTCCCTTAAACCTCCGACTTTCTCTAATGGCGCCAAGAAGTTTCATGGCGTTGGTCTCATAAGAGCTGTTGCTGCGGAGCAGCAGGTAGAGAAGACCAAGGTTGCTCTTGTCAGAATTGGCACCAGAGGAAGGTAACTTTTTTGTTTTGTGCGGCTTTTTCAGATTCTCTTTTGCTTTATAGTTTTGGGTTGACTTTAGTTTCTAATTTTGAGTTTTTGGGTGATTTGAGTTTGAAAATGTGGAGGATTGAATTATTGTTGAAGGGTTTGGATCAGTTCAATTTGTGTCTTTCAATTGGGATATAATTTTGCTGTATTGTAGAATTGTTAGGAAGTACAGTATGTTTCTCGTGTTTGTACTTGAATCTATCAAAACTTGTATGTTTACGTTTGTCCAAATGAAGTAGTATGGAAGTATTTTTTGTGTGCTTGTAAATCAGACCATGAAGAACACGAAAGGGTGGAAAATGAACAAAATTAAAGAAAGTAATAGATTCCTGAGAACTATCAATTGTGGAGAACTTCCTTCCTTACGAATGTGCTTAACTTCTGAATTGATTTAATTCGGATAAAGTATTCATGTTGCTCATGCCATCAAATTTAGGATCCAATCTGCTGCTGTTAGAGTAGAACGAGTTCTTCATAAGAAGAGTAAGTCTAAGAGGGGGTTCGAATTCAAAGTTTTCACGTTTATCTTTGGATTTCATGTTTCTTCATTTTCTAAGTTTCATGTTTCTCCTTAGGCTTTCGAGTTGTTTTTTGTGACTGTATAGGTAGTATTTTGCTTGATTGCTCCTTTTTCCTAGAAGGTCCCCTCTTTTGTAGGCTTGGTTTTTTATTTCATTTTTTCTAAAAGAAAACAGAAAGTAAAAAAAAAAAAGTTTATTCTAGTAGGAGTTAGGGGGACATTTAATTTTCTTGTTGAAACTGTGAACTATGTTGATAAACAGTCTACTTCATTTTCTATATGCTTGTTTGAGCTAAATCTTCTTTTGGGTTGCTTCATCTTCTTCTGTGTCATATATAATTTTCTGTGTGTAGGACAGCCCATTAGCACTTGCCCAGGCTCATGAGACGAGAGACAAACTCATGGCTTCCCATCCTGAGCTAGCCGAAGATGGGGCCATTCAGATTGTTGTAATCAAAACAACTGGTGACAAAATACTCTCTCAGCCACTTGCAGACATTGGTGGGAAAGGCTTATTTACTAAAGAGATCGATGATGCACTTATTAATGGTGACATAGACATTGCTGTTCACTCAATGAAAGATGTACCAACTTACTTGCCTGAAAAAACCATCCTCCCATGTAACCTTCCGAGGGAGGATGTCCGCGATGCATTCATTTCTCTGAGTGCAGGTTCATTTGCTGAGCTTCCAGCTGGAAGCATCATTGGTACAGCTTCACTGAGAAGAAAGTCTCAGCTACTGAATAGATATCCATCCCTCAAAGTAAGTATGCTATGGTTCTACGTTTGCTAGGTTGTTGATCGCAGTGACTGATGTGTGAAATAAGCTCCTAGAAAATATGATAGAAAGAATGTCTCTTTGGATTTCGTTCTAAATTGGTTGATCCATAAACCCATAAACTCCATTGCAGTTGTGTTGTCTATGTTCTCAATAAATTCCAACCTTTTCCTTCTTAGTCATTATAATACAATGGGGTGTCATATTGTTTGACAACGATAGTTTATTTTCTTATTTGAGATTGGAAGATTCCCTGCTGCTCCCATCTGTCCATTCTTTTAATCTAGAATGCCCAACTCTTGATGCAACATTTACGACTATGATTTTAAAGATGTTTGAAGAGGATTAAAATCTCATTCTCTTAGATTGATTCTGACACTCTCTCTCGCATTGGTGTCATCTGTTAGGTGCTGGAGAATTTTCGGGGCAATGTCCAAACAAGGTTGAGAAAACTGAATGAAGGAGTGGTCCAAGCAACACTGTTGGCTCTAGCTGGACTTCGGCGTTTAAATATGACAGAAAATGTTACTTCGATCCTTTCAATCGATGAAATGCTTCCAGCTGTTGCTCAGGGAGCTATTGGGATTGCCTGTCGAAGTGACGATGACATAATGGTGTGTTTTCATTAGCTATCGAAATTGATGACCCATGTTCGTCTGGCAATTGCCATTATAACTATTATCTTGTTGAGAAAACCATCGTTCCTATTCAAGTGGCTGACTTCTTATCTCCGTATAGGCTTATACGATTAAAATTCATTGCTTGGAAAAATGCTTAATGTCTCTCTTGTTTGATTTCTCATTGTGCAGGCCAATTACTTAGCCTCATTAAACCATGAGGAAACAAGACTGGCTGTTGTTTGTGAGAGAGCTTTTCTTGAGACTCTTGATGGATCTTGCCGAACTCCGATAGCAGGATATGCTTCCAGAGATGAAGATGGCAATTGTATATTTAAAGGGCTGGTGGCTTCCCCAGATGGAACCCGAGGTACTACGATCAACATGCATGTTCATATGAAATAACTTCTACCCTTGTTTTCGTTAGCTTCTTCTCTCCATTGATAGACCATTGGTCATCTTCATCTTTTGATCCTTACCCTTGTTTCTTCCCTTTCATTTCAGTTCTTGAAACTTCTCGACGAGGTCCATACGCCATTGAAGATATGATTGCAATGGGAAAGGATGCTGGCCAGGAGCTTCTTTCTCGAGCAGGTCCCGGTTTTTTTGATAGCTAGTTAGTTTAACATAGGATTTTAGGATAAGGGCAGAGAGAGAAGATCAAGGCTTCGTATCCAAATCTTCAATTGAGTAGGAGTTTTATTCATCGTTTGAGATGAAGCTTGTTGTATTTTAAATATGATTTTTTCCCACTCTTCGTTGGTTGTGTAAATTAGAACAGAAAACATTCAGACTATCTTGGCTTCCCCCAAATAATCAATACAATTTTCCTCATCTGTGTGCGAA

mRNA sequence

GAAATGGATGAGAATCCTATCCGCCAACGCAAATGCAGACATTTGCCCTGTTGGGTCATCAAAAACAATCCCTTCTAAGCTAACCCATTTCCCCTTTTTGCTTTCTTTCTTCTTCTCCTCCTCCTCCTCCTCCTCCTCATCTCTCTCAATCTCAAACAAACACTCTCTCAAATGGGTGTTTTATCTTCCCCTTCTGTAAGCCACTCTCCAATGCCTCGCCCTTGTAATGTTGGTTCTCTTTCTCTTCTTGGGTTCTCTTCACTTTCCCTTAAACCTCCGACTTTCTCTAATGGCGCCAAGAAGTTTCATGGCGTTGGTCTCATAAGAGCTGTTGCTGCGGAGCAGCAGGTAGAGAAGACCAAGGTTGCTCTTGTCAGAATTGGCACCAGAGGAAGCCCATTAGCACTTGCCCAGGCTCATGAGACGAGAGACAAACTCATGGCTTCCCATCCTGAGCTAGCCGAAGATGGGGCCATTCAGATTGTTGTAATCAAAACAACTGGTGACAAAATACTCTCTCAGCCACTTGCAGACATTGGTGGGAAAGGCTTATTTACTAAAGAGATCGATGATGCACTTATTAATGGTGACATAGACATTGCTGTTCACTCAATGAAAGATGTACCAACTTACTTGCCTGAAAAAACCATCCTCCCATGTAACCTTCCGAGGGAGGATGTCCGCGATGCATTCATTTCTCTGAGTGCAGGTTCATTTGCTGAGCTTCCAGCTGGAAGCATCATTGGTACAGCTTCACTGAGAAGAAAGTCTCAGCTACTGAATAGATATCCATCCCTCAAAGTGCTGGAGAATTTTCGGGGCAATGTCCAAACAAGGTTGAGAAAACTGAATGAAGGAGTGGTCCAAGCAACACTGTTGGCTCTAGCTGGACTTCGGCGTTTAAATATGACAGAAAATGTTACTTCGATCCTTTCAATCGATGAAATGCTTCCAGCTGTTGCTCAGGGAGCTATTGGGATTGCCTGTCGAAGTGACGATGACATAATGGCCAATTACTTAGCCTCATTAAACCATGAGGAAACAAGACTGGCTGTTGTTTGTGAGAGAGCTTTTCTTGAGACTCTTGATGGATCTTGCCGAACTCCGATAGCAGGATATGCTTCCAGAGATGAAGATGGCAATTGTATATTTAAAGGGCTGGTGGCTTCCCCAGATGGAACCCGAGTTCTTGAAACTTCTCGACGAGGTCCATACGCCATTGAAGATATGATTGCAATGGGAAAGGATGCTGGCCAGGAGCTTCTTTCTCGAGCAGGTCCCGGTTTTTTTGATAGCTAGTTAGTTTAACATAGGATTTTAGGATAAGGGCAGAGAGAGAAGATCAAGGCTTCGTATCCAAATCTTCAATTGAGTAGGAGTTTTATTCATCGTTTGAGATGAAGCTTGTTGTATTTTAAATATGATTTTTTCCCACTCTTCGTTGGTTGTGTAAATTAGAACAGAAAACATTCAGACTATCTTGGCTTCCCCCAAATAATCAATACAATTTTCCTCATCTGTGTGCGAA

Coding sequence (CDS)

ATGGGTGTTTTATCTTCCCCTTCTGTAAGCCACTCTCCAATGCCTCGCCCTTGTAATGTTGGTTCTCTTTCTCTTCTTGGGTTCTCTTCACTTTCCCTTAAACCTCCGACTTTCTCTAATGGCGCCAAGAAGTTTCATGGCGTTGGTCTCATAAGAGCTGTTGCTGCGGAGCAGCAGGTAGAGAAGACCAAGGTTGCTCTTGTCAGAATTGGCACCAGAGGAAGCCCATTAGCACTTGCCCAGGCTCATGAGACGAGAGACAAACTCATGGCTTCCCATCCTGAGCTAGCCGAAGATGGGGCCATTCAGATTGTTGTAATCAAAACAACTGGTGACAAAATACTCTCTCAGCCACTTGCAGACATTGGTGGGAAAGGCTTATTTACTAAAGAGATCGATGATGCACTTATTAATGGTGACATAGACATTGCTGTTCACTCAATGAAAGATGTACCAACTTACTTGCCTGAAAAAACCATCCTCCCATGTAACCTTCCGAGGGAGGATGTCCGCGATGCATTCATTTCTCTGAGTGCAGGTTCATTTGCTGAGCTTCCAGCTGGAAGCATCATTGGTACAGCTTCACTGAGAAGAAAGTCTCAGCTACTGAATAGATATCCATCCCTCAAAGTGCTGGAGAATTTTCGGGGCAATGTCCAAACAAGGTTGAGAAAACTGAATGAAGGAGTGGTCCAAGCAACACTGTTGGCTCTAGCTGGACTTCGGCGTTTAAATATGACAGAAAATGTTACTTCGATCCTTTCAATCGATGAAATGCTTCCAGCTGTTGCTCAGGGAGCTATTGGGATTGCCTGTCGAAGTGACGATGACATAATGGCCAATTACTTAGCCTCATTAAACCATGAGGAAACAAGACTGGCTGTTGTTTGTGAGAGAGCTTTTCTTGAGACTCTTGATGGATCTTGCCGAACTCCGATAGCAGGATATGCTTCCAGAGATGAAGATGGCAATTGTATATTTAAAGGGCTGGTGGCTTCCCCAGATGGAACCCGAGTTCTTGAAACTTCTCGACGAGGTCCATACGCCATTGAAGATATGATTGCAATGGGAAAGGATGCTGGCCAGGAGCTTCTTTCTCGAGCAGGTCCCGGTTTTTTTGATAGCTAG

Protein sequence

MGVLSSPSVSHSPMPRPCNVGSLSLLGFSSLSLKPPTFSNGAKKFHGVGLIRAVAAEQQVEKTKVALVRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSAGSFAELPAGSIIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALAGLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDIMANYLASLNHEETRLAVVCERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIEDMIAMGKDAGQELLSRAGPGFFDS*

Homology

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BLAST of CSPI03G29370 vs. ExPASy Swiss-Prot
Match: Q43082 (Porphobilinogen deaminase, chloroplastic OS=Pisum sativum OX=3888 GN=HEMC PE=1 SV=1)

HSP 1 Score: 553.9 bits (1426), Expect = 1.4e-156
Identity = 284/349 (81.38%), Postives = 314/349 (89.97%), Query Frame = 0


Query: 30  SLSLKPPTFSNGAKK---FHGVGLIRAVAAEQQVEKTKVALVRIGTRGSPLALAQAHETR 89
           SLSL   +F   + K   F    +  ++A EQQ ++ K AL+RIGTRGSPLALAQAHETR
Sbjct: 21  SLSLFTSSFRFSSFKTSPFSKCRIRASLAVEQQTQQNKTALIRIGTRGSPLALAQAHETR 80

Query: 90  DKLMASHPELAEDGAIQIVVIKTTGDKILSQPLADIGGKGLFTKEIDDALINGDIDIAVH 149
           DKLMASH ELAE+GAIQIV+IKTTGDKILSQPLADIGGKGLFTKEID+ALINGDIDIAVH
Sbjct: 81  DKLMASHTELAEEGAIQIVIIKTTGDKILSQPLADIGGKGLFTKEIDEALINGDIDIAVH 140

Query: 150 SMKDVPTYLPEKTILPCNLPREDVRDAFISLSAGSFAELPAGSIIGTASLRRKSQLLNRY 209
           SMKDVPTYLPE+TILPCNLPREDVRDAFISLSA S A+LPAGS+IGTASLRRKSQ+L+RY
Sbjct: 141 SMKDVPTYLPEETILPCNLPREDVRDAFISLSAASLADLPAGSVIGTASLRRKSQILHRY 200

Query: 210 PSLKVLENFRGNVQTRLRKLNEGVVQATLLALAGLRRLNMTENVTSILSIDEMLPAVAQG 269
           PSL V +NFRGNVQTRLRKL+EGVV+ATLLALAGL+RLNMTENVTS LSID+MLPAVAQG
Sbjct: 201 PSLTVQDNFRGNVQTRLRKLSEGVVKATLLALAGLKRLNMTENVTSTLSIDDMLPAVAQG 260

Query: 270 AIGIACRSDDDIMANYLASLNHEETRLAVVCERAFLETLDGSCRTPIAGYASRDEDGNCI 329
           AIGIACRS+DD MA YLASLNHEETRLA+ CERAFL TLDGSCRTPIAGYASRD+DGNC+
Sbjct: 261 AIGIACRSNDDKMAEYLASLNHEETRLAISCERAFLTTLDGSCRTPIAGYASRDKDGNCL 320

Query: 330 FKGLVASPDGTRVLETSRRGPYAIEDMIAMGKDAGQELLSRAGPGFFDS 376
           F+GLVASPDGTRVLETSR G Y  EDM+ +GKDAG+ELLSRAGPGFF+S
Sbjct: 321 FRGLVASPDGTRVLETSRIGSYTYEDMMKIGKDAGEELLSRAGPGFFNS 369

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BLAST of CSPI03G29370 vs. ExPASy Swiss-Prot
Match: Q43316 (Porphobilinogen deaminase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=HEMC PE=1 SV=1)

HSP 1 Score: 549.7 bits (1415), Expect = 2.6e-155
Identity = 275/356 (77.25%), Postives = 310/356 (87.08%), Query Frame = 0


Query: 18  CNVGSLSLLGFSSLSLKPPTFSNGAKKFHGVGLIRAVAAEQQVEKTKVALVRIGTRGSPL 77
           C++GS+S +GFS   +  P      +K    G ++A  A +Q  KT+ A++RIGTRGSPL
Sbjct: 27  CSLGSVSAIGFSLPQISSPALGKCRRKQSSSGFVKACVAVEQ--KTRTAIIRIGTRGSPL 86

Query: 78  ALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPLADIGGKGLFTKEIDDALI 137
           ALAQA+ETR+KL   HPEL EDGAI I +IKTTGDKILSQPLADIGGKGLFTKEID+ALI
Sbjct: 87  ALAQAYETREKLKKKHPELVEDGAIHIEIIKTTGDKILSQPLADIGGKGLFTKEIDEALI 146

Query: 138 NGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSAGSFAELPAGSIIGTASLR 197
           NG IDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFI L+A + AELPAGS++GTASLR
Sbjct: 147 NGHIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFICLTAATLAELPAGSVVGTASLR 206

Query: 198 RKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALAGLRRLNMTENVTSILSID 257
           RKSQ+L++YP+L V ENFRGNVQTRL KL  G VQATLLALAGL+RL+MTENV SILS+D
Sbjct: 207 RKSQILHKYPALHVEENFRGNVQTRLSKLQGGKVQATLLALAGLKRLSMTENVASILSLD 266

Query: 258 EMLPAVAQGAIGIACRSDDDIMANYLASLNHEETRLAVVCERAFLETLDGSCRTPIAGYA 317
           EMLPAVAQGAIGIACR+DDD MA YLASLNHEETRLA+ CERAFLETLDGSCRTPIAGYA
Sbjct: 267 EMLPAVAQGAIGIACRTDDDKMATYLASLNHEETRLAISCERAFLETLDGSCRTPIAGYA 326

Query: 318 SRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIEDMIAMGKDAGQELLSRAGPGFF 374
           S+DE+GNCIF+GLVASPDGT+VLETSR+GPY  EDM+ MGKDAGQELLSRAGPGFF
Sbjct: 327 SKDEEGNCIFRGLVASPDGTKVLETSRKGPYVYEDMVKMGKDAGQELLSRAGPGFF 380

[Close]

BLAST of CSPI03G29370 vs. ExPASy Swiss-Prot
Match: Q6H6D2 (Porphobilinogen deaminase, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=HEMC PE=2 SV=1)

HSP 1 Score: 500.0 bits (1286), Expect = 2.4e-140
Identity = 249/325 (76.62%), Postives = 282/325 (86.77%), Query Frame = 0


Query: 51  IRAVAAEQQVEKTKVALVRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTT 110
           +RA  A Q   + KV+L+RIGTRGSPLALAQAHETRDKL A+H ELAE+GA++IV+IKTT
Sbjct: 33  VRAAVAVQAEAQAKVSLIRIGTRGSPLALAQAHETRDKLKAAHSELAEEGAVEIVIIKTT 92

Query: 111 GDKILSQPLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDV 170
           GD IL +PLADIGGKGLFTKEIDDAL+ G IDIAVHSMKDVPTYLPE TILPCNLPREDV
Sbjct: 93  GDMILDKPLADIGGKGLFTKEIDDALLQGRIDIAVHSMKDVPTYLPEGTILPCNLPREDV 152

Query: 171 RDAFISLSAGSFAELPAGSIIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGV 230
           RDAFI L+A S AELPAGS++G+ASLRR+SQ+L +YPSLKV+ NFRGNVQTRLRKL EG 
Sbjct: 153 RDAFICLTASSLAELPAGSVVGSASLRRQSQILYKYPSLKVV-NFRGNVQTRLRKLKEGD 212

Query: 231 VQATLLALAGLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDIMANYLASLNHEE 290
           V ATLLALAGL+RLNM E  TS+LS+DEMLPAVAQGAIGIACRS DD M NYL+SLNHE+
Sbjct: 213 VHATLLALAGLKRLNMAETATSVLSVDEMLPAVAQGAIGIACRSSDDTMMNYLSSLNHED 272

Query: 291 TRLAVVCERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAI 350
           TRLAV CER FL  LDG+CRTPIA YASRD+DGNC F+GL+ASPDG+ V ETSR GPY  
Sbjct: 273 TRLAVACEREFLSVLDGNCRTPIAAYASRDKDGNCSFRGLLASPDGSTVYETSRTGPYDF 332

Query: 351 EDMIAMGKDAGQELLSRAGPGFFDS 376
           + M+ MGKDAG EL ++AGPGFFDS
Sbjct: 333 DIMVEMGKDAGHELKAKAGPGFFDS 356

[Close]

BLAST of CSPI03G29370 vs. ExPASy Swiss-Prot
Match: A1B853 (Porphobilinogen deaminase OS=Paracoccus denitrificans (strain Pd 1222) OX=318586 GN=hemC PE=3 SV=1)

HSP 1 Score: 318.9 bits (816), Expect = 7.5e-86
Identity = 174/307 (56.68%), Postives = 219/307 (71.34%), Query Frame = 0


Query: 68  VRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPLADIGGKGL 127
           +RIGTRGS LALAQAHETRD+LMA+H  LA D A +IVVIKTTGD++L +PL +IGGKGL
Sbjct: 11  IRIGTRGSALALAQAHETRDRLMAAH-GLAAD-AFRIVVIKTTGDRVLDRPLKEIGGKGL 70

Query: 128 FTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSAGSFAELPA 187
           FT+EI+DAL+  +IDIAVHSMKD+PT  PE  ++ C LPREDVRDAF+S    + +ELP 
Sbjct: 71  FTREIEDALLAHEIDIAVHSMKDMPTIQPEGLVIDCYLPREDVRDAFVSAQFAAISELPQ 130

Query: 188 GSIIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALAGLRRLNMT 247
           G+++G++SLRR++QL  R P LK++E FRGNVQTRL+KL +GV  AT LA+AGL RL M 
Sbjct: 131 GAVVGSSSLRRRAQLAARRPDLKLVE-FRGNVQTRLKKLEDGVAVATFLAMAGLTRLGML 190

Query: 248 ENVTSILSIDEMLPAVAQGAIGIACRSDDDIMANYLASLNHEETRLAVVCERAFLETLDG 307
                 +  DEMLPAVAQG IG+  R+DD   A+ LA+++  ++ L V  ERAFL  LDG
Sbjct: 191 HVARGAVEPDEMLPAVAQGCIGVERRADDARTASLLAAISDRDSALRVTAERAFLARLDG 250

Query: 308 SCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIEDMIAMGKDAGQELLSR 367
           SC+TPIAG A    D     +G +  PDG+ V+   R GP A  D  AMG D  +EL  R
Sbjct: 251 SCQTPIAGLAELQGD-RLRLRGEILRPDGSEVIAAERVGPAA--DGAAMGTDLAEELRGR 310

Query: 368 AGPGFFD 375
           A   FFD
Sbjct: 311 APADFFD 311

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BLAST of CSPI03G29370 vs. ExPASy Swiss-Prot
Match: Q3SVF3 (Porphobilinogen deaminase OS=Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255) OX=323098 GN=hemC PE=3 SV=1)

HSP 1 Score: 313.2 bits (801), Expect = 4.1e-84
Identity = 174/316 (55.06%), Postives = 214/316 (67.72%), Query Frame = 0


Query: 58  QQVEKTKVALVRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQ 117
           Q  ++T + L  IGTRGSPLALAQAHE RD+L  +H    E   I I  I+T+GD I  +
Sbjct: 2   QSSDETDI-LATIGTRGSPLALAQAHEVRDRLARAHQVAPE--RIAIKTIRTSGDAIQDR 61

Query: 118 PLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISL 177
           PL D+GGKGLFTKEI++AL+ G ID AVHS KDVPT+LP+ T LP  LPREDVRD FIS 
Sbjct: 62  PLFDVGGKGLFTKEIEEALLAGTIDFAVHSSKDVPTFLPDATWLPAFLPREDVRDVFISP 121

Query: 178 SAGSFAELPAGSIIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLA 237
            AGS  +LPAG+ +GTASLRR++ +L   P LKV  + RGNV+TRLRK++ G   ATLLA
Sbjct: 122 HAGSLNDLPAGATVGTASLRRQAMVLKLRPDLKV-NSLRGNVETRLRKISVGEADATLLA 181

Query: 238 LAGLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDIMANYLASLNHEETRLAVVC 297
           LAGL RL + +  T IL  DE LPAV QGAI I  R DDD +  ++ ++   ET +A+  
Sbjct: 182 LAGLNRLGLQDKATRILETDEFLPAVGQGAIAIESRRDDDRINAFVKAIGDPETEVALSA 241

Query: 298 ERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIEDMIAMG 357
           ER+FL  LDGSCRTPI G+   + D    F+GL+ SPDGT   ET+R G  A  D  A+G
Sbjct: 242 ERSFLALLDGSCRTPIGGHCRVNGD-RIDFRGLIISPDGTEFYETTREGARA--DAAALG 301

Query: 358 KDAGQELLSRAGPGFF 374
            DA  EL  RAG  FF
Sbjct: 302 ADAAHELRERAGEKFF 310

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BLAST of CSPI03G29370 vs. ExPASy TrEMBL
Match: A0A6J1GCC7 (Hydroxymethylbilane synthase OS=Cucurbita moschata OX=3662 GN=LOC111452689 PE=3 SV=1)

HSP 1 Score: 683.7 bits (1763), Expect = 4.3e-193
Identity = 351/376 (93.35%), Postives = 358/376 (95.21%), Query Frame = 0


Query: 1   MGVLSSPSVSHSPMPRPCNVGSLSLLGFSSLSLKPPTFSNGAKKFHGVGLIRA-VAAEQQ 60
           MGV SSPS+SH PM RPCN+GSLS  GFSSLSLKPP FS G KKFHG GLIRA VA EQQ
Sbjct: 1   MGVFSSPSISHYPMARPCNLGSLSRHGFSSLSLKPPAFSTGGKKFHGFGLIRASVAVEQQ 60

Query: 61  VEKTKVALVRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL 120
            EKTKVALVRIGTRGSPLALAQAHETR+KLMA HPELAEDGAIQIV+IKTTGDKILSQPL
Sbjct: 61  AEKTKVALVRIGTRGSPLALAQAHETREKLMAIHPELAEDGAIQIVIIKTTGDKILSQPL 120

Query: 121 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSA 180
           ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLS 
Sbjct: 121 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSV 180

Query: 181 GSFAELPAGSIIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALA 240
           GS AELPAGS+IGTASLRRKSQLLNRYPSL VLENFRGNVQTRLRKLNEGVVQATLLALA
Sbjct: 181 GSLAELPAGSVIGTASLRRKSQLLNRYPSLNVLENFRGNVQTRLRKLNEGVVQATLLALA 240

Query: 241 GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDIMANYLASLNHEETRLAVVCER 300
           GLRRLNMTE VTSILSIDEMLPAVAQGAIGIACRSDDD MANYLASLNHEETRLAVVCER
Sbjct: 241 GLRRLNMTETVTSILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHEETRLAVVCER 300

Query: 301 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIEDMIAMGKD 360
           AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAI+DMIAMGKD
Sbjct: 301 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIDDMIAMGKD 360

Query: 361 AGQELLSRAGPGFFDS 376
           AG+ELLSRAGPGFFDS
Sbjct: 361 AGKELLSRAGPGFFDS 376

[Close]

BLAST of CSPI03G29370 vs. ExPASy TrEMBL
Match: A0A6J1KA97 (Hydroxymethylbilane synthase OS=Cucurbita maxima OX=3661 GN=LOC111493083 PE=3 SV=1)

HSP 1 Score: 683.3 bits (1762), Expect = 5.6e-193
Identity = 350/376 (93.09%), Postives = 359/376 (95.48%), Query Frame = 0


Query: 1   MGVLSSPSVSHSPMPRPCNVGSLSLLGFSSLSLKPPTFSNGAKKFHGVGLIRA-VAAEQQ 60
           MGV SSPS+SH PM RPCN+GSLS  GFSSLSLKPP FS G K+FHGVGLIRA VA EQQ
Sbjct: 1   MGVFSSPSISHYPMARPCNLGSLSRHGFSSLSLKPPAFSTGDKRFHGVGLIRASVAVEQQ 60

Query: 61  VEKTKVALVRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL 120
            EKTKVALVRIGTRGSPLALAQAHETR+KLMA HPELAEDGAIQIV+IKTTGDKILSQPL
Sbjct: 61  AEKTKVALVRIGTRGSPLALAQAHETREKLMAIHPELAEDGAIQIVIIKTTGDKILSQPL 120

Query: 121 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSA 180
           ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLS 
Sbjct: 121 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSV 180

Query: 181 GSFAELPAGSIIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALA 240
           GS AELPAGS+IGTASLRRKSQLLNRYPSL VLENFRGNVQTRLRKLNEGVVQATLLALA
Sbjct: 181 GSLAELPAGSVIGTASLRRKSQLLNRYPSLNVLENFRGNVQTRLRKLNEGVVQATLLALA 240

Query: 241 GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDIMANYLASLNHEETRLAVVCER 300
           GLRRLNMTE VTSILSIDEMLPAVAQGAIGIACRSDDD MANYLASLNHEETRLAVVCER
Sbjct: 241 GLRRLNMTETVTSILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHEETRLAVVCER 300

Query: 301 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIEDMIAMGKD 360
           AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAI+DM+AMGKD
Sbjct: 301 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIDDMVAMGKD 360

Query: 361 AGQELLSRAGPGFFDS 376
           AG+ELLSRAGPGFFDS
Sbjct: 361 AGKELLSRAGPGFFDS 376

[Close]

BLAST of CSPI03G29370 vs. ExPASy TrEMBL
Match: A0A6J1IZW2 (Hydroxymethylbilane synthase OS=Cucurbita maxima OX=3661 GN=LOC111481450 PE=3 SV=1)

HSP 1 Score: 678.7 bits (1750), Expect = 1.4e-191
Identity = 350/376 (93.09%), Postives = 360/376 (95.74%), Query Frame = 0


Query: 1   MGVLSSPSVSHSPMPRPCNVGSLSLLGFSSLSLKPPTFSNGAKKFHGVGLIRA-VAAEQQ 60
           M  LSSPSVS+SPM RPCN+ SLSL GFSSLSLKPP F+N  KKFHG GLIRA VA EQQ
Sbjct: 1   MVALSSPSVSNSPMARPCNLASLSLPGFSSLSLKPPAFTNAPKKFHGFGLIRASVAVEQQ 60

Query: 61  VEKTKVALVRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL 120
            EKTKVAL+RIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL
Sbjct: 61  PEKTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL 120

Query: 121 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSA 180
           ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISL A
Sbjct: 121 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLRA 180

Query: 181 GSFAELPAGSIIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALA 240
           GS AELPAGS++GTASLRRKSQLLNRYPSL+VLENFRGNVQTRL+KLNEGVVQATLLALA
Sbjct: 181 GSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQTRLKKLNEGVVQATLLALA 240

Query: 241 GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDIMANYLASLNHEETRLAVVCER 300
           GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDD MANYLASLNHEETRLAVVCER
Sbjct: 241 GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHEETRLAVVCER 300

Query: 301 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIEDMIAMGKD 360
           AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRG YAI+DMIAMGKD
Sbjct: 301 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGSYAIDDMIAMGKD 360

Query: 361 AGQELLSRAGPGFFDS 376
           AG+ELLSRAGPGFFDS
Sbjct: 361 AGKELLSRAGPGFFDS 376

[Close]

BLAST of CSPI03G29370 vs. ExPASy TrEMBL
Match: A0A6J1DII9 (Hydroxymethylbilane synthase OS=Momordica charantia OX=3673 GN=LOC111020357 PE=3 SV=1)

HSP 1 Score: 676.4 bits (1744), Expect = 6.9e-191
Identity = 345/376 (91.76%), Postives = 359/376 (95.48%), Query Frame = 0


Query: 1   MGVLSSPSVSHSPMPRPCNVGSLSLLGFSSLSLKPPTFSNGAKKFHGVGLIRA-VAAEQQ 60
           MGV SSP ++HSPM RPCNVGSLS LGFSSLSLKPP F NG KKF G+GL+RA VA EQQ
Sbjct: 1   MGVFSSPPITHSPMARPCNVGSLSPLGFSSLSLKPPAFCNGGKKFGGLGLVRASVAVEQQ 60

Query: 61  VEKTKVALVRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL 120
            EKTKVALVRIGTRGSPLALAQA+ETRDKLMA HPELAEDGAIQI++IKTTGDKILSQPL
Sbjct: 61  TEKTKVALVRIGTRGSPLALAQAYETRDKLMAIHPELAEDGAIQIIIIKTTGDKILSQPL 120

Query: 121 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSA 180
           ADIGGKGLFTKEIDDALIN DIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSA
Sbjct: 121 ADIGGKGLFTKEIDDALINSDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSA 180

Query: 181 GSFAELPAGSIIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALA 240
           GS AELPAGS+IGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALA
Sbjct: 181 GSLAELPAGSVIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALA 240

Query: 241 GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDIMANYLASLNHEETRLAVVCER 300
           GLRRLNMTENVTS+LSIDEMLPAVAQGAIGIACRS+DD MANYLASLNHEETRLAVVCER
Sbjct: 241 GLRRLNMTENVTSVLSIDEMLPAVAQGAIGIACRSNDDKMANYLASLNHEETRLAVVCER 300

Query: 301 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIEDMIAMGKD 360
           AFLE LDGSCRTPIAGYAS+DEDGNCIFKGLVASPDGTRVLETSRRG YAI+DM+AMGKD
Sbjct: 301 AFLEMLDGSCRTPIAGYASKDEDGNCIFKGLVASPDGTRVLETSRRGAYAIDDMVAMGKD 360

Query: 361 AGQELLSRAGPGFFDS 376
           AG+ELLSRAGPGFFDS
Sbjct: 361 AGKELLSRAGPGFFDS 376

[Close]

BLAST of CSPI03G29370 vs. ExPASy TrEMBL
Match: A0A6J1F354 (Hydroxymethylbilane synthase OS=Cucurbita moschata OX=3662 GN=LOC111441758 PE=3 SV=1)

HSP 1 Score: 675.2 bits (1741), Expect = 1.5e-190
Identity = 348/376 (92.55%), Postives = 357/376 (94.95%), Query Frame = 0


Query: 1   MGVLSSPSVSHSPMPRPCNVGSLSLLGFSSLSLKPPTFSNGAKKFHGVGLIRA-VAAEQQ 60
           M  LSSPSVSHSPM  PCN+ SLSL GFSSLSLKPP F+NG KK HG GLIRA VA EQQ
Sbjct: 1   MVALSSPSVSHSPMAHPCNLASLSLPGFSSLSLKPPAFTNGPKKLHGFGLIRASVAVEQQ 60

Query: 61  VEKTKVALVRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL 120
             KTKVAL+RIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL
Sbjct: 61  AGKTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL 120

Query: 121 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSA 180
           ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISL A
Sbjct: 121 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLRA 180

Query: 181 GSFAELPAGSIIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALA 240
           GS AELPAGS++GTASLRRKSQLLNRYPSL+VLENFRGNVQTRL+KLNEGVVQATLLALA
Sbjct: 181 GSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQTRLKKLNEGVVQATLLALA 240

Query: 241 GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDIMANYLASLNHEETRLAVVCER 300
           GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDD MA YLASLNHEETRLAVVCER
Sbjct: 241 GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMAEYLASLNHEETRLAVVCER 300

Query: 301 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIEDMIAMGKD 360
           AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRG YAI+DMIAMGKD
Sbjct: 301 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGSYAIDDMIAMGKD 360

Query: 361 AGQELLSRAGPGFFDS 376
           AG+ELLSRAGPGFFDS
Sbjct: 361 AGKELLSRAGPGFFDS 376

[Close]

BLAST of CSPI03G29370 vs. NCBI nr
Match: KGN58748.2 (hypothetical protein Csa_002701 [Cucumis sativus])

HSP 1 Score: 728.0 bits (1878), Expect = 4.1e-206
Identity = 373/375 (99.47%), Postives = 374/375 (99.73%), Query Frame = 0


Query: 1   MGVLSSPSVSHSPMPRPCNVGSLSLLGFSSLSLKPPTFSNGAKKFHGVGLIRAVAAEQQV 60
           MGVLSSPSVSHSPMPRPCNVGSLS LGFSSLSLKPPTFSNGAKKFHGVGLIRAVAAEQQV
Sbjct: 57  MGVLSSPSVSHSPMPRPCNVGSLSFLGFSSLSLKPPTFSNGAKKFHGVGLIRAVAAEQQV 116

Query: 61  EKTKVALVRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPLA 120
           EKTKVAL+RIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPLA
Sbjct: 117 EKTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPLA 176

Query: 121 DIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSAG 180
           DIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSAG
Sbjct: 177 DIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSAG 236

Query: 181 SFAELPAGSIIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALAG 240
           SFAELPAGSIIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALAG
Sbjct: 237 SFAELPAGSIIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALAG 296

Query: 241 LRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDIMANYLASLNHEETRLAVVCERA 300
           LRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDIMANYLASLNHEETRLAVVCERA
Sbjct: 297 LRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDIMANYLASLNHEETRLAVVCERA 356

Query: 301 FLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIEDMIAMGKDA 360
           FLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIEDMIAMGKDA
Sbjct: 357 FLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIEDMIAMGKDA 416

Query: 361 GQELLSRAGPGFFDS 376
           GQELLSRAGPGFFDS
Sbjct: 417 GQELLSRAGPGFFDS 431

[Close]

BLAST of CSPI03G29370 vs. NCBI nr
Match: XP_004149732.1 (porphobilinogen deaminase, chloroplastic [Cucumis sativus])

HSP 1 Score: 728.0 bits (1878), Expect = 4.1e-206
Identity = 373/375 (99.47%), Postives = 374/375 (99.73%), Query Frame = 0


Query: 1   MGVLSSPSVSHSPMPRPCNVGSLSLLGFSSLSLKPPTFSNGAKKFHGVGLIRAVAAEQQV 60
           MGVLSSPSVSHSPMPRPCNVGSLS LGFSSLSLKPPTFSNGAKKFHGVGLIRAVAAEQQV
Sbjct: 1   MGVLSSPSVSHSPMPRPCNVGSLSFLGFSSLSLKPPTFSNGAKKFHGVGLIRAVAAEQQV 60

Query: 61  EKTKVALVRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPLA 120
           EKTKVAL+RIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPLA
Sbjct: 61  EKTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPLA 120

Query: 121 DIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSAG 180
           DIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSAG
Sbjct: 121 DIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSAG 180

Query: 181 SFAELPAGSIIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALAG 240
           SFAELPAGSIIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALAG
Sbjct: 181 SFAELPAGSIIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALAG 240

Query: 241 LRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDIMANYLASLNHEETRLAVVCERA 300
           LRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDIMANYLASLNHEETRLAVVCERA
Sbjct: 241 LRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDIMANYLASLNHEETRLAVVCERA 300

Query: 301 FLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIEDMIAMGKDA 360
           FLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIEDMIAMGKDA
Sbjct: 301 FLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIEDMIAMGKDA 360

Query: 361 GQELLSRAGPGFFDS 376
           GQELLSRAGPGFFDS
Sbjct: 361 GQELLSRAGPGFFDS 375

[Close]

BLAST of CSPI03G29370 vs. NCBI nr
Match: XP_038906286.1 (porphobilinogen deaminase, chloroplastic [Benincasa hispida])

HSP 1 Score: 705.7 bits (1820), Expect = 2.2e-199
Identity = 365/376 (97.07%), Postives = 368/376 (97.87%), Query Frame = 0


Query: 1   MGVLSSPSVSHSPMPRPCNVGSLSLLGFSSLSLKPPTFSNGAKKFHGVGLIRA-VAAEQQ 60
           MGVLSSPSVSHSPMPRP NVGSLSLLGFSSLSLKPP FSNGAKKFHG GLIRA VA EQQ
Sbjct: 1   MGVLSSPSVSHSPMPRPRNVGSLSLLGFSSLSLKPPAFSNGAKKFHGFGLIRASVAVEQQ 60

Query: 61  VEKTKVALVRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL 120
            EKTKVALVRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL
Sbjct: 61  TEKTKVALVRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL 120

Query: 121 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSA 180
           ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSA
Sbjct: 121 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSA 180

Query: 181 GSFAELPAGSIIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALA 240
           GS AELPAGSIIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALA
Sbjct: 181 GSLAELPAGSIIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALA 240

Query: 241 GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDIMANYLASLNHEETRLAVVCER 300
           GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACR+DDD MANYLASLNHEETRLAVVCER
Sbjct: 241 GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRTDDDKMANYLASLNHEETRLAVVCER 300

Query: 301 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIEDMIAMGKD 360
           AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAI+DMIAMGKD
Sbjct: 301 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIDDMIAMGKD 360

Query: 361 AGQELLSRAGPGFFDS 376
           AG+ELLSRAGPGFFDS
Sbjct: 361 AGKELLSRAGPGFFDS 376

[Close]

BLAST of CSPI03G29370 vs. NCBI nr
Match: XP_023523547.1 (porphobilinogen deaminase, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 685.3 bits (1767), Expect = 3.0e-193
Identity = 352/376 (93.62%), Postives = 359/376 (95.48%), Query Frame = 0


Query: 1   MGVLSSPSVSHSPMPRPCNVGSLSLLGFSSLSLKPPTFSNGAKKFHGVGLIRA-VAAEQQ 60
           MGV SSPS+SH PM RPCN+GSLS  GFSSLSLKPP FS GAKKFHG GLIRA VA EQQ
Sbjct: 18  MGVFSSPSISHYPMARPCNLGSLSRHGFSSLSLKPPAFSTGAKKFHGFGLIRASVAVEQQ 77

Query: 61  VEKTKVALVRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL 120
            EKTKVALVRIGTRGSPLALAQAHETR+KLMA HPELAEDGAIQIV+IKTTGDKILSQPL
Sbjct: 78  AEKTKVALVRIGTRGSPLALAQAHETREKLMAIHPELAEDGAIQIVIIKTTGDKILSQPL 137

Query: 121 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSA 180
           ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLS 
Sbjct: 138 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSV 197

Query: 181 GSFAELPAGSIIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALA 240
           GS AELPAGS+IGTASLRRKSQLLNRYPSL VLENFRGNVQTRLRKLNEGVVQATLLALA
Sbjct: 198 GSLAELPAGSVIGTASLRRKSQLLNRYPSLNVLENFRGNVQTRLRKLNEGVVQATLLALA 257

Query: 241 GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDIMANYLASLNHEETRLAVVCER 300
           GLRRLNMTE VTSILSIDEMLPAVAQGAIGIACRSDDD MANYLASLNHEETRLAVVCER
Sbjct: 258 GLRRLNMTETVTSILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHEETRLAVVCER 317

Query: 301 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIEDMIAMGKD 360
           AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAI+DMIAMGKD
Sbjct: 318 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIDDMIAMGKD 377

Query: 361 AGQELLSRAGPGFFDS 376
           AG+ELLSRAGPGFFDS
Sbjct: 378 AGKELLSRAGPGFFDS 393

[Close]

BLAST of CSPI03G29370 vs. NCBI nr
Match: XP_022949290.1 (porphobilinogen deaminase, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 683.7 bits (1763), Expect = 8.9e-193
Identity = 351/376 (93.35%), Postives = 358/376 (95.21%), Query Frame = 0


Query: 1   MGVLSSPSVSHSPMPRPCNVGSLSLLGFSSLSLKPPTFSNGAKKFHGVGLIRA-VAAEQQ 60
           MGV SSPS+SH PM RPCN+GSLS  GFSSLSLKPP FS G KKFHG GLIRA VA EQQ
Sbjct: 1   MGVFSSPSISHYPMARPCNLGSLSRHGFSSLSLKPPAFSTGGKKFHGFGLIRASVAVEQQ 60

Query: 61  VEKTKVALVRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL 120
            EKTKVALVRIGTRGSPLALAQAHETR+KLMA HPELAEDGAIQIV+IKTTGDKILSQPL
Sbjct: 61  AEKTKVALVRIGTRGSPLALAQAHETREKLMAIHPELAEDGAIQIVIIKTTGDKILSQPL 120

Query: 121 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSA 180
           ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLS 
Sbjct: 121 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSV 180

Query: 181 GSFAELPAGSIIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALA 240
           GS AELPAGS+IGTASLRRKSQLLNRYPSL VLENFRGNVQTRLRKLNEGVVQATLLALA
Sbjct: 181 GSLAELPAGSVIGTASLRRKSQLLNRYPSLNVLENFRGNVQTRLRKLNEGVVQATLLALA 240

Query: 241 GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDIMANYLASLNHEETRLAVVCER 300
           GLRRLNMTE VTSILSIDEMLPAVAQGAIGIACRSDDD MANYLASLNHEETRLAVVCER
Sbjct: 241 GLRRLNMTETVTSILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHEETRLAVVCER 300

Query: 301 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIEDMIAMGKD 360
           AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAI+DMIAMGKD
Sbjct: 301 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIDDMIAMGKD 360

Query: 361 AGQELLSRAGPGFFDS 376
           AG+ELLSRAGPGFFDS
Sbjct: 361 AGKELLSRAGPGFFDS 376

[Close]

BLAST of CSPI03G29370 vs. TAIR 10
Match: AT5G08280.1 (hydroxymethylbilane synthase )

HSP 1 Score: 549.7 bits (1415), Expect = 1.9e-156
Identity = 275/356 (77.25%), Postives = 310/356 (87.08%), Query Frame = 0


Query: 18  CNVGSLSLLGFSSLSLKPPTFSNGAKKFHGVGLIRAVAAEQQVEKTKVALVRIGTRGSPL 77
           C++GS+S +GFS   +  P      +K    G ++A  A +Q  KT+ A++RIGTRGSPL
Sbjct: 27  CSLGSVSAIGFSLPQISSPALGKCRRKQSSSGFVKACVAVEQ--KTRTAIIRIGTRGSPL 86

Query: 78  ALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPLADIGGKGLFTKEIDDALI 137
           ALAQA+ETR+KL   HPEL EDGAI I +IKTTGDKILSQPLADIGGKGLFTKEID+ALI
Sbjct: 87  ALAQAYETREKLKKKHPELVEDGAIHIEIIKTTGDKILSQPLADIGGKGLFTKEIDEALI 146

Query: 138 NGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSAGSFAELPAGSIIGTASLR 197
           NG IDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFI L+A + AELPAGS++GTASLR
Sbjct: 147 NGHIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFICLTAATLAELPAGSVVGTASLR 206

Query: 198 RKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALAGLRRLNMTENVTSILSID 257
           RKSQ+L++YP+L V ENFRGNVQTRL KL  G VQATLLALAGL+RL+MTENV SILS+D
Sbjct: 207 RKSQILHKYPALHVEENFRGNVQTRLSKLQGGKVQATLLALAGLKRLSMTENVASILSLD 266

Query: 258 EMLPAVAQGAIGIACRSDDDIMANYLASLNHEETRLAVVCERAFLETLDGSCRTPIAGYA 317
           EMLPAVAQGAIGIACR+DDD MA YLASLNHEETRLA+ CERAFLETLDGSCRTPIAGYA
Sbjct: 267 EMLPAVAQGAIGIACRTDDDKMATYLASLNHEETRLAISCERAFLETLDGSCRTPIAGYA 326

Query: 318 SRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIEDMIAMGKDAGQELLSRAGPGFF 374
           S+DE+GNCIF+GLVASPDGT+VLETSR+GPY  EDM+ MGKDAGQELLSRAGPGFF
Sbjct: 327 SKDEEGNCIFRGLVASPDGTKVLETSRKGPYVYEDMVKMGKDAGQELLSRAGPGFF 380

The following BLAST results are available for this feature:

BLAST of CSPI03G29370 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q43082	1.4e-156	81.38	Porphobilinogen deaminase, chloroplastic OS=Pisum sativum OX=3888 GN=HEMC PE=1 S...	[more]
Q43316	2.6e-155	77.25	Porphobilinogen deaminase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=HEMC...	[more]
Q6H6D2	2.4e-140	76.62	Porphobilinogen deaminase, chloroplastic OS=Oryza sativa subsp. japonica OX=3994...	[more]
A1B853	7.5e-86	56.68	Porphobilinogen deaminase OS=Paracoccus denitrificans (strain Pd 1222) OX=318586...	[more]
Q3SVF3	4.1e-84	55.06	Porphobilinogen deaminase OS=Nitrobacter winogradskyi (strain ATCC 25391 / DSM 1...	[more]

BLAST of CSPI03G29370 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GCC7	4.3e-193	93.35	Hydroxymethylbilane synthase OS=Cucurbita moschata OX=3662 GN=LOC111452689 PE=3 ...	[more]
A0A6J1KA97	5.6e-193	93.09	Hydroxymethylbilane synthase OS=Cucurbita maxima OX=3661 GN=LOC111493083 PE=3 SV...	[more]
A0A6J1IZW2	1.4e-191	93.09	Hydroxymethylbilane synthase OS=Cucurbita maxima OX=3661 GN=LOC111481450 PE=3 SV...	[more]
A0A6J1DII9	6.9e-191	91.76	Hydroxymethylbilane synthase OS=Momordica charantia OX=3673 GN=LOC111020357 PE=3...	[more]
A0A6J1F354	1.5e-190	92.55	Hydroxymethylbilane synthase OS=Cucurbita moschata OX=3662 GN=LOC111441758 PE=3 ...	[more]

BLAST of CSPI03G29370 vs. NCBI nr


Match Name	E-value	Identity	Description
KGN58748.2	4.1e-206	99.47	hypothetical protein Csa_002701 [Cucumis sativus]	[more]
XP_004149732.1	4.1e-206	99.47	porphobilinogen deaminase, chloroplastic [Cucumis sativus]	[more]
XP_038906286.1	2.2e-199	97.07	porphobilinogen deaminase, chloroplastic [Benincasa hispida]	[more]
XP_023523547.1	3.0e-193	93.62	porphobilinogen deaminase, chloroplastic-like [Cucurbita pepo subsp. pepo]	[more]
XP_022949290.1	8.9e-193	93.35	porphobilinogen deaminase, chloroplastic-like [Cucurbita moschata]	[more]

BLAST of CSPI03G29370 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G08280.1	1.9e-156	77.25	hydroxymethylbilane synthase	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000860	Porphobilinogen deaminase	PRINTS	PR00151	PORPHBDMNASE	coord: 142..161 score: 61.19 coord: 111..131 score: 66.21 coord: 297..314 score: 53.17 coord: 190..207 score: 54.23 coord: 210..227 score: 53.97
IPR000860	Porphobilinogen deaminase	PIRSF	PIRSF001438	PBGD	coord: 50..375 e-value: 5.5E-116 score: 384.9
IPR000860	Porphobilinogen deaminase	TIGRFAM	TIGR00212	TIGR00212	coord: 68..367 e-value: 6.0E-100 score: 332.0
IPR000860	Porphobilinogen deaminase	PANTHER	PTHR11557	PORPHOBILINOGEN DEAMINASE	coord: 15..374
IPR000860	Porphobilinogen deaminase	HAMAP	MF_00260	Porphobil_deam	coord: 67..367 score: 34.067959
None	No IPR available	GENE3D	3.40.190.10		coord: 68..277 e-value: 1.2E-81 score: 275.0
None	No IPR available	GENE3D	3.40.190.10		coord: 169..269 e-value: 1.2E-81 score: 275.0
None	No IPR available	PANTHER	PTHR11557:SF8	BNAC02G01240D PROTEIN	coord: 15..374
None	No IPR available	CDD	cd13648	PBP2_PBGD_1	coord: 67..346 e-value: 0.0 score: 515.809
None	No IPR available	SUPERFAMILY	53850	Periplasmic binding protein-like II	coord: 68..285
IPR022418	Porphobilinogen deaminase, C-terminal	PFAM	PF03900	Porphobil_deamC	coord: 293..365 e-value: 3.6E-15 score: 56.1
IPR022417	Porphobilinogen deaminase, N-terminal	PFAM	PF01379	Porphobil_deam	coord: 68..278 e-value: 2.0E-75 score: 252.8
IPR036803	Porphobilinogen deaminase, C-terminal domain superfamily	GENE3D	3.30.160.40		coord: 288..374 e-value: 3.2E-24 score: 87.1
IPR036803	Porphobilinogen deaminase, C-terminal domain superfamily	SUPERFAMILY	54782	Porphobilinogen deaminase (hydroxymethylbilane synthase), C-terminal domain	coord: 287..374
IPR022419	Porphobilinogen deaminase, dipyrromethane cofactor binding site	PROSITE	PS00533	PORPHOBILINOGEN_DEAM	coord: 298..314

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G29370.1	CSPI03G29370.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0018160	peptidyl-pyrromethane cofactor linkage
biological_process	GO:0006782	protoporphyrinogen IX biosynthetic process
biological_process	GO:0050790	regulation of catalytic activity
biological_process	GO:0017157	regulation of exocytosis
biological_process	GO:0033014	tetrapyrrole biosynthetic process
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0005096	GTPase activator activity
molecular_function	GO:0004418	hydroxymethylbilane synthase activity
molecular_function	GO:0045159	myosin II binding
molecular_function	GO:0019905	syntaxin binding