CSPI03G29340 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI03G29340
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionCytochrome P450
LocationChr3: 26913470 .. 26915238 (-)
RNA-Seq ExpressionCSPI03G29340
SyntenyCSPI03G29340
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTAAAGAGTAGGTATTTCTCATGTGCGTTTTTGCACCCTTTCTCTGTTAGCGTTAGCCCATCACCATGTTGAGCTCCGGAAACCTCACCGCTATCTCCTCCGGTGAATTCCCATCTCAGAGATTGGTTGCATGGAATCTCTTCTTCTTACCCGACATTCAAATGTTAGAGATATTCATAGCAATTTTTATTTTTACAGCAACAAGATCATTGAGGCAAAAAAGAAACAGGGGCTACCTGTGTGGCCAGTTGTCGGAATGTTACCGTCTTTAGTCACTGGACTCCGAAGCAACTTGTATGAATGGATCACCGAAGTGCTATGCCGTCAAAATGGAACTTTCAGATTCAAAGGGCCATGGTTCAGTAGCTTGAATTGGGTTGTAACTTCAGATCCTCGTAACATTGAGCACATTCTGAAAACAGAGTTTCTCAAGTACCCAAAAGGCTCCTTCTTCCGTGAAACAGTCCATGACCTTCTCGGCGACGGAATCTTCAACGCGGACGGCGAGATTTGGCACAAGCAGAGGAAGACAACCAGCATCGAATTCCATTCAGCAAAGTTCCGACAACTCACTGTGGAGTCCCTCCACGAGTTAGTCCACTCTCGGCTCCTCCCTATTTTACACAGACATTTCGTCGAACACTTGGTGCCCATTGATCTCCAAGACATTCTTCTACGCCTAACATTCGACAATGTCTGTATGATCGCATTCGGCGTTGAACCCGGCTGTTTGAACCCTAATTTACCAGTGATTCCCTTCGCCCACGCCTTCGAGGTGGCAACGGAAGCGACCGTTCTTCGATTCGTAACACCGCAATTCTTATGGAAAACAATGAGGCATTTCAATTTAGGAACAGAAAAGAGTCTCAAATTCGCCCTAAAAGAGGTCAATGAATTCGCAATGAACGTAATTGAAAAGAGAAGAAGAGAGAAGGTCAATGAAACCGAGAGATCGGACCTGTTATCAGTGTTTACGGGACTGAAGGACGAGAACGGGGAGCCGTATTCGGACAAGTTCTTGAAAGATATATGCGTGAATTTTATACTTGCCGGAAGAGATACGTCGTCAGTGGCGTCGAGTTGGTTCTTTTGGCTGATTGATCGAAATCCAGAAGTGGAAGAGAGAATTGTGGAGAAGATCTGCCGGATATTGAAGAAGAGAGGAGAGGCGGCGGGGGAATCGTCGGGCGGCAATGTGACGTTTGAGGTAGAAGAGATAAAGAAGATGGAATATTTGCAAGCCGCTCTTTCTGAAGCTTTGAGATTGTTCCCTTCTGTGCCTGTTGATCACAAGGAGGTGAGTTCTTCTTATTTATACCTTTCCTCATCTCATGGGGTTGGGTCCTTTTTCAAATAAACAATTTGCAAATGTGACCTCGACATTTTTGTAGTTCAAGAATTGTAGGGTGGAGACTGAATTATCCAACTTTTGAGAGTAATTAGTGTGGTCTCGAGAGTAACCATGTAGGAGTTTTTATTTATTTTCTCAATATTGATATAGCATTACTTACTATCATTTAAACCCAATGGTTAGGTCATTCAGGATGATGTATTACCAAATGGAACAGAGCTAAAGAAGGGAACAAAACTGGTCTATGCAATATATGCAATGGGTAGAACGGAGGCAATATGGGGAAAAGATTGTTATGAATTCAAGCCAGAGAGATGGTTAAGAGATAGTCGATTTACGGCCGTGCTTGGGAAAAGATTTTGCATACTACCAAATGAAGTACATTTCTGCCTCTGTCATCCTCCGCTACCGTGTTAG

mRNA sequence

CTTAAAGAGTAGGTATTTCTCATGTGCGTTTTTGCACCCTTTCTCTGTTAGCGTTAGCCCATCACCATGTTGAGCTCCGGAAACCTCACCGCTATCTCCTCCGGTGAATTCCCATCTCAGAGATTGGTTGCATGGAATCTCTTCTTCTTACCCGACATTCAAATGTTAGAGATATTCATAGCAATTTTTATTTTTACAGCAACAAGATCATTGAGGCAAAAAAGAAACAGGGGCTACCTGTGTGGCCAGTTGTCGGAATGTTACCGTCTTTAGTCACTGGACTCCGAAGCAACTTGTATGAATGGATCACCGAAGTGCTATGCCGTCAAAATGGAACTTTCAGATTCAAAGGGCCATGGTTCAGTAGCTTGAATTGGGTTGTAACTTCAGATCCTCGTAACATTGAGCACATTCTGAAAACAGAGTTTCTCAAGTACCCAAAAGGCTCCTTCTTCCGTGAAACAGTCCATGACCTTCTCGGCGACGGAATCTTCAACGCGGACGGCGAGATTTGGCACAAGCAGAGGAAGACAACCAGCATCGAATTCCATTCAGCAAAGTTCCGACAACTCACTGTGGAGTCCCTCCACGAGTTAGTCCACTCTCGGCTCCTCCCTATTTTACACAGACATTTCGTCGAACACTTGGTGCCCATTGATCTCCAAGACATTCTTCTACGCCTAACATTCGACAATGTCTGTATGATCGCATTCGGCGTTGAACCCGGCTGTTTGAACCCTAATTTACCAGTGATTCCCTTCGCCCACGCCTTCGAGGTGGCAACGGAAGCGACCGTTCTTCGATTCGTAACACCGCAATTCTTATGGAAAACAATGAGGCATTTCAATTTAGGAACAGAAAAGAGTCTCAAATTCGCCCTAAAAGAGGTCAATGAATTCGCAATGAACGTAATTGAAAAGAGAAGAAGAGAGAAGGTCAATGAAACCGAGAGATCGGACCTGTTATCAGTGTTTACGGGACTGAAGGACGAGAACGGGGAGCCGTATTCGGACAAGTTCTTGAAAGATATATGCGTGAATTTTATACTTGCCGGAAGAGATACGTCGTCAGTGGCGTCGAGTTGGTTCTTTTGGCTGATTGATCGAAATCCAGAAGTGGAAGAGAGAATTGTGGAGAAGATCTGCCGGATATTGAAGAAGAGAGGAGAGGCGGCGGGGGAATCGTCGGGCGGCAATGTGACGTTTGAGGTAGAAGAGATAAAGAAGATGGAATATTTGCAAGCCGCTCTTTCTGAAGCTTTGAGATTGTTCCCTTCTGTGCCTGTTGATCACAAGGAGGATGATGTATTACCAAATGGAACAGAGCTAAAGAAGGGAACAAAACTGGTCTATGCAATATATGCAATGGGTAGAACGGAGGCAATATGGGGAAAAGATTGTTATGAATTCAAGCCAGAGAGATGGTTAAGAGATAGTCGATTTACGGCCGTGCTTGGGAAAAGATTTTGCATACTACCAAATGAAGTACATTTCTGCCTCTGTCATCCTCCGCTACCGTGTTAG

Coding sequence (CDS)

ATGGAATCTCTTCTTCTTACCCGACATTCAAATGTTAGAGATATTCATAGCAATTTTTATTTTTACAGCAACAAGATCATTGAGGCAAAAAAGAAACAGGGGCTACCTGTGTGGCCAGTTGTCGGAATGTTACCGTCTTTAGTCACTGGACTCCGAAGCAACTTGTATGAATGGATCACCGAAGTGCTATGCCGTCAAAATGGAACTTTCAGATTCAAAGGGCCATGGTTCAGTAGCTTGAATTGGGTTGTAACTTCAGATCCTCGTAACATTGAGCACATTCTGAAAACAGAGTTTCTCAAGTACCCAAAAGGCTCCTTCTTCCGTGAAACAGTCCATGACCTTCTCGGCGACGGAATCTTCAACGCGGACGGCGAGATTTGGCACAAGCAGAGGAAGACAACCAGCATCGAATTCCATTCAGCAAAGTTCCGACAACTCACTGTGGAGTCCCTCCACGAGTTAGTCCACTCTCGGCTCCTCCCTATTTTACACAGACATTTCGTCGAACACTTGGTGCCCATTGATCTCCAAGACATTCTTCTACGCCTAACATTCGACAATGTCTGTATGATCGCATTCGGCGTTGAACCCGGCTGTTTGAACCCTAATTTACCAGTGATTCCCTTCGCCCACGCCTTCGAGGTGGCAACGGAAGCGACCGTTCTTCGATTCGTAACACCGCAATTCTTATGGAAAACAATGAGGCATTTCAATTTAGGAACAGAAAAGAGTCTCAAATTCGCCCTAAAAGAGGTCAATGAATTCGCAATGAACGTAATTGAAAAGAGAAGAAGAGAGAAGGTCAATGAAACCGAGAGATCGGACCTGTTATCAGTGTTTACGGGACTGAAGGACGAGAACGGGGAGCCGTATTCGGACAAGTTCTTGAAAGATATATGCGTGAATTTTATACTTGCCGGAAGAGATACGTCGTCAGTGGCGTCGAGTTGGTTCTTTTGGCTGATTGATCGAAATCCAGAAGTGGAAGAGAGAATTGTGGAGAAGATCTGCCGGATATTGAAGAAGAGAGGAGAGGCGGCGGGGGAATCGTCGGGCGGCAATGTGACGTTTGAGGTAGAAGAGATAAAGAAGATGGAATATTTGCAAGCCGCTCTTTCTGAAGCTTTGAGATTGTTCCCTTCTGTGCCTGTTGATCACAAGGAGGATGATGTATTACCAAATGGAACAGAGCTAAAGAAGGGAACAAAACTGGTCTATGCAATATATGCAATGGGTAGAACGGAGGCAATATGGGGAAAAGATTGTTATGAATTCAAGCCAGAGAGATGGTTAAGAGATAGTCGATTTACGGCCGTGCTTGGGAAAAGATTTTGCATACTACCAAATGAAGTACATTTCTGCCTCTGTCATCCTCCGCTACCGTGTTAG

Protein sequence

MESLLLTRHSNVRDIHSNFYFYSNKIIEAKKKQGLPVWPVVGMLPSLVTGLRSNLYEWITEVLCRQNGTFRFKGPWFSSLNWVVTSDPRNIEHILKTEFLKYPKGSFFRETVHDLLGDGIFNADGEIWHKQRKTTSIEFHSAKFRQLTVESLHELVHSRLLPILHRHFVEHLVPIDLQDILLRLTFDNVCMIAFGVEPGCLNPNLPVIPFAHAFEVATEATVLRFVTPQFLWKTMRHFNLGTEKSLKFALKEVNEFAMNVIEKRRREKVNETERSDLLSVFTGLKDENGEPYSDKFLKDICVNFILAGRDTSSVASSWFFWLIDRNPEVEERIVEKICRILKKRGEAAGESSGGNVTFEVEEIKKMEYLQAALSEALRLFPSVPVDHKEDDVLPNGTELKKGTKLVYAIYAMGRTEAIWGKDCYEFKPERWLRDSRFTAVLGKRFCILPNEVHFCLCHPPLPC*
Homology
BLAST of CSPI03G29340 vs. ExPASy Swiss-Prot
Match: Q9FMY1 (Cytochrome P450 86B1 OS=Arabidopsis thaliana OX=3702 GN=CYP86B1 PE=2 SV=1)

HSP 1 Score: 589.3 bits (1518), Expect = 3.7e-167
Identity = 287/445 (64.49%), Postives = 345/445 (77.53%), Query Frame = 0

Query: 19  FYFYSNKIIEAKKKQGLPVWPVVGMLPSLVTGLRSNLYEWITEVLCRQNGTFRFKGPWFS 78
           F F +   +  KK QGLPVWP +GMLPSL  GLR N+YEW+++VLC QNGTF+F+GPWFS
Sbjct: 46  FVFVAIHALRQKKYQGLPVWPFLGMLPSLAFGLRGNIYEWLSDVLCLQNGTFQFRGPWFS 105

Query: 79  SLNWVVTSDPRNIEHILKTEFLKYPKGSFFRETVHDLLGDGIFNADGEIWHKQRKTTSIE 138
           SLN  +T DPRN+EH+LK  F  +PKGS+FR+ + DLLGDGIFNAD E W +QRKT SIE
Sbjct: 106 SLNSTITCDPRNVEHLLKNRFSVFPKGSYFRDNLRDLLGDGIFNADDETWQRQRKTASIE 165

Query: 139 FHSAKFRQLTVESLHELVHSRLLPILHRHFVEHLVPIDLQDILLRLTFDNVCMIAFGVEP 198
           FHSAKFRQLT +SL ELVH RLLP+L    V+   PIDLQD+LLRLTFDNVCMIAFGV+P
Sbjct: 166 FHSAKFRQLTTQSLFELVHKRLLPVLETS-VKSSSPIDLQDVLLRLTFDNVCMIAFGVDP 225

Query: 199 GCLNPNLPVIPFAHAFEVATEATVLRFVTPQFLWKTMRHFNLGTEKSLKFALKEVNEFAM 258
           GCL P+ PVIPFA AFE ATEA V+RFV P  +WK MR+ ++GTEK LK ++K V++FA 
Sbjct: 226 GCLGPDQPVIPFAKAFEDATEAAVVRFVMPTCVWKFMRYLDIGTEKKLKESIKGVDDFAD 285

Query: 259 NVIEKRRREKVNE---TERSDLLSVFTGLKDENGEPYSDKFLKDICVNFILAGRDTSSVA 318
            VI  R++E   E   T+RSDLL+VF GL+DE GE +SDKFL+DICVNFILAGRDTSSVA
Sbjct: 286 EVIRTRKKELSLEGETTKRSDLLTVFMGLRDEKGESFSDKFLRDICVNFILAGRDTSSVA 345

Query: 319 SSWFFWLIDRNPEVEERIVEKICRILKKRGE-AAGESSGGNVTFEVEEIKKMEYLQAALS 378
            SWFFWL+++NPEVEE+I+ ++C+IL++R +    E S     F  EEIKKM+YLQAALS
Sbjct: 346 LSWFFWLLEKNPEVEEKIMVEMCKILRQRDDHGNAEKSDYEPVFGPEEIKKMDYLQAALS 405

Query: 379 EALRLFPSVPVDHK---EDDVLPNGTELKKGTKLVYAIYAMGRTEAIWGKDCYEFKPERW 438
           EALRL+PSVPVDHK   EDDV P+GT LKKG K++YAIYAMGR EAIWGKDC EF+PERW
Sbjct: 406 EALRLYPSVPVDHKEVQEDDVFPDGTMLKKGDKVIYAIYAMGRMEAIWGKDCLEFRPERW 465

Query: 439 LRDSRFTAVLGKRFCILPNEVHFCL 457
           LRD RF +    +F         CL
Sbjct: 466 LRDGRFMSESAYKFTAFNGGPRLCL 489

BLAST of CSPI03G29340 vs. ExPASy Swiss-Prot
Match: Q9CAD6 (Cytochrome P450 86A7 OS=Arabidopsis thaliana OX=3702 GN=CYP86A7 PE=2 SV=1)

HSP 1 Score: 360.5 bits (924), Expect = 2.8e-98
Identity = 193/417 (46.28%), Postives = 267/417 (64.03%), Query Frame = 0

Query: 33  QGLPVWPVVGMLPSLVTGLRSNLYEWITEVLCRQNGTFR---FKGPWFSSL--NWVVTSD 92
           +G  VWP+VG LP+L+T     ++++I + L    GT++   F  P+ +    +  VT D
Sbjct: 28  KGPRVWPLVGSLPALITNAH-RMHDFIADNLRMCGGTYQTCIFPIPFLAKKQGHVTVTCD 87

Query: 93  PRNIEHILKTEFLKYPKGSFFRETVHDLLGDGIFNADGEIWHKQRKTTSIEFHSAKFRQL 152
           P+N+EHILKT F  YPKG  ++   HDLLGDGIFN+DG+ W  QRKT ++EF +   RQ 
Sbjct: 88  PKNLEHILKTRFDNYPKGPSWQSVFHDLLGDGIFNSDGDTWRFQRKTAALEFTTRTLRQA 147

Query: 153 TVESLHELVHSRLLPILHRHFVEHLVPIDLQDILLRLTFDNVCMIAFGVEPGCLNPNLPV 212
               +   + +RL+PIL         PIDLQD+LLRLTFDN+C + FG +P  L+P  P 
Sbjct: 148 MARWVDRAIKNRLVPIL-ESARSRAEPIDLQDVLLRLTFDNICGLTFGKDPRTLSPEFPE 207

Query: 213 IPFAHAFEVATEATVLRFVTPQFLWKTMRHFNLGTEKSLKFALKEVNEFAMNVIEKRRRE 272
             FA AF+ ATEAT+ RF+ P+F+WK  +   LG E  +  ++  V+ +   +I  R+ E
Sbjct: 208 NGFAVAFDGATEATLQRFIMPEFIWKIRKWLRLGLEDDMSRSISHVDNYLSEIINTRKLE 267

Query: 273 ----KVNETERSDLLSVFTGLKDENGEPYSDKFLKDICVNFILAGRDTSSVASSWFFWLI 332
               + +E+   DLLS F   K    E YSDK+LK + +NFILAGRDTSSVA SWFFWL+
Sbjct: 268 LLGQQQDESRHDDLLSRFMKKK----ESYSDKYLKYVALNFILAGRDTSSVAMSWFFWLV 327

Query: 333 DRNPEVEERIVEKICRIL-KKRGEAAGESSGGNVTFEVEEIKKMEYLQAALSEALRLFPS 392
             NP VEE+I+ +IC IL K R     + +   +TF  +EI ++ YL+AALSE LRL+PS
Sbjct: 328 SLNPRVEEKIINEICTILIKTRDTNVSKWTDEPLTF--DEIDQLVYLKAALSETLRLYPS 387

Query: 393 VPVDHK---EDDVLPNGTELKKGTKLVYAIYAMGRTEAIWGKDCYEFKPERWLRDSR 437
           VP D K    +DVLP+GT +  G+ + Y+IY++GR + IWG+DC EFKPERWL +SR
Sbjct: 388 VPEDSKFVVANDVLPDGTFVPSGSNVTYSIYSVGRMKFIWGEDCLEFKPERWLEESR 436

BLAST of CSPI03G29340 vs. ExPASy Swiss-Prot
Match: B3RFJ6 (Cytochrome P450 86A22 OS=Petunia hybrida OX=4102 GN=CYP86A22 PE=1 SV=1)

HSP 1 Score: 356.7 bits (914), Expect = 4.0e-97
Identity = 194/415 (46.75%), Postives = 266/415 (64.10%), Query Frame = 0

Query: 30  KKKQGLPVWPVVGMLPSLVTGLRSNLYEWITEVLCRQNGTFR---FKGPWFSSLNWV-VT 89
           K  +G  +WPVVG LP L+    + ++EWI + L   +GT++      P+ +    V VT
Sbjct: 28  KGPKGPTMWPVVGSLPGLIEN-GNRMHEWIADNLRACSGTYQTCICAIPFLARKQLVTVT 87

Query: 90  SDPRNIEHILKTEFLKYPKGSFFRETVHDLLGDGIFNADGEIWHKQRKTTSIEFHSAKFR 149
            DP+N+EHILK  F  YPKG  ++   HDLLG+GIFN+DG+ W  QRKT ++EF +   R
Sbjct: 88  CDPKNLEHILKVRFDNYPKGPTWQAVFHDLLGEGIFNSDGDTWLFQRKTAALEFTTRTLR 147

Query: 150 QLTVESLHELVHSRLLPILHRHFVEHLVPIDLQDILLRLTFDNVCMIAFGVEPGCLNPNL 209
           Q     ++  + +R  PIL    V+   P+DLQD+LLRLTFDN+C +AFG +P  L+P L
Sbjct: 148 QAMGRWVNRAIKNRFCPILEMAQVQG-KPVDLQDLLLRLTFDNICGLAFGKDPETLSPEL 207

Query: 210 PVIPFAHAFEVATEATVLRFVTPQFLWKTMRHFNLGTEKSLKFALKEVNEFAMNVIEKRR 269
           P   FA +F+ ATEAT+ RF+ P+F+WK  +   LG E SL  +LK+V+ +  +VI  R+
Sbjct: 208 PENNFATSFDRATEATLHRFIMPEFVWKLKKMLGLGLEVSLNNSLKQVDNYMTDVINTRK 267

Query: 270 REKVNE----TERSDLLSVFTGLKDENGEPYSDKFLKDICVNFILAGRDTSSVASSWFFW 329
            E +N      +  DLLS F   K    E YSDKFL+ + +NFILAGRDTSSVA SWFFW
Sbjct: 268 LELLNHQNGGPQHDDLLSRFMKKK----ESYSDKFLQHVALNFILAGRDTSSVALSWFFW 327

Query: 330 LIDRNPEVEERIVEKICRIL-KKRGEAAGESSGGNVTFEVEEIKKMEYLQAALSEALRLF 389
           L+  NP VEE+I+ +IC IL + RG    +     + F  EE+ ++ YL+AALSE LRL+
Sbjct: 328 LVSSNPRVEEKILVEICTILAETRGNDTSKWLEEPLVF--EEVDQLMYLKAALSETLRLY 387

Query: 390 PSVPVDHK---EDDVLPNGTELKKGTKLVYAIYAMGRTEAIWGKDCYEFKPERWL 433
           PSVP D K    DD LP+GT +  G+ + Y+IY+ GR + IWG+DC EFKPERW+
Sbjct: 388 PSVPEDSKHVISDDYLPDGTFVPAGSNITYSIYSTGRMKFIWGEDCLEFKPERWM 434

BLAST of CSPI03G29340 vs. ExPASy Swiss-Prot
Match: Q9LMM1 (Cytochrome P450 86A4 OS=Arabidopsis thaliana OX=3702 GN=CYP86A4 PE=1 SV=1)

HSP 1 Score: 345.1 bits (884), Expect = 1.2e-93
Identity = 186/414 (44.93%), Postives = 260/414 (62.80%), Query Frame = 0

Query: 33  QGLPVWPVVGMLPSLVTGLRSNLYEWITEVLCRQNGTFR---FKGPWFSSLNWV--VTSD 92
           +G  VWP++G LP L+   R  ++EWITE L    GT++   F  P+ +    +  VT D
Sbjct: 28  KGPRVWPLLGSLPGLIE-QRDRMHEWITENLRACGGTYQTCIFAVPFLAKKQGLVTVTCD 87

Query: 93  PRNIEHILKTEFLKYPKGSFFRETVHDLLGDGIFNADGEIWHKQRKTTSIEFHSAKFRQL 152
           P+N+EH+LKT F  YPKG  ++   HDLLG GIFN+DG+ W  QRKT ++EF +   RQ 
Sbjct: 88  PKNLEHMLKTRFDNYPKGPTWQSVFHDLLGQGIFNSDGDTWLFQRKTAALEFTTRTLRQA 147

Query: 153 TVESLHELVHSRLLPILHRHFVEHLVPIDLQDILLRLTFDNVCMIAFGVEPGCLNPNLPV 212
               ++  +  R  PIL     ++  P+DLQD++LRLTFDN+C +AFG +     P LP 
Sbjct: 148 MGRWVNRGIKLRFCPIL-ATAQDNAEPVDLQDLILRLTFDNICGLAFGKDTRTCAPGLPE 207

Query: 213 IPFAHAFEVATEATVLRFVTPQFLWKTMRHFNLGTEKSLKFALKEVNEFAMNVIEKRRRE 272
             FA AF+ ATEA++ RF+ P+F+WK  +   LG E SL  +L E++E+   VI  R++E
Sbjct: 208 NGFASAFDRATEASLQRFIIPKFMWKLKKWLGLGLEVSLSRSLGEIDEYLAAVINTRKQE 267

Query: 273 KVNETE------RSDLLSVFTGLKDENGEPYSDKFLKDICVNFILAGRDTSSVASSWFFW 332
            +++ E        DLLS F   K E+   YSD FL+ + +NFILAGRDTSSVA SWFFW
Sbjct: 268 LMSQQESGTHQRHDDLLSRFMMKKTES---YSDTFLQHVALNFILAGRDTSSVALSWFFW 327

Query: 333 LIDRNPEVEERIVEKICRILKKRGEAAGESSGGNVTFEVEEIKKMEYLQAALSEALRLFP 392
           LI  +P VE++IV +IC +L +       +S        +EI ++ YL+AA+SE LRL+P
Sbjct: 328 LITMHPTVEDKIVREICSVLIETRGTDDVASWTEEPLGFDEIDRLVYLKAAISETLRLYP 387

Query: 393 SVPVDHK---EDDVLPNGTELKKGTKLVYAIYAMGRTEAIWGKDCYEFKPERWL 433
           SVP D K    DDVLP+GT +  G+ + Y+IYA GR ++ WG+DC EF PERW+
Sbjct: 388 SVPEDSKHVENDDVLPDGTFVPAGSSVTYSIYAAGRMKSTWGEDCLEFNPERWI 436

BLAST of CSPI03G29340 vs. ExPASy Swiss-Prot
Match: O23066 (Cytochrome P450 86A2 OS=Arabidopsis thaliana OX=3702 GN=CYP86A2 PE=1 SV=1)

HSP 1 Score: 344.7 bits (883), Expect = 1.6e-93
Identity = 193/440 (43.86%), Postives = 268/440 (60.91%), Query Frame = 0

Query: 33  QGLPVWPVVGMLPSLVTGLRSNLYEWITEVLCRQNGTFR---FKGPWFSSLNWV--VTSD 92
           +G  VWPV+G LP L+   R  +++WITE L    GT++      P+ +    +  VT D
Sbjct: 28  KGPRVWPVLGSLPGLIE-QRDRMHDWITENLRACGGTYQTCICAVPFLAKKQGLVTVTCD 87

Query: 93  PRNIEHILKTEFLKYPKGSFFRETVHDLLGDGIFNADGEIWHKQRKTTSIEFHSAKFRQL 152
           P+NIEH+LKT F  YPKG  ++   HD LG GIFN+DG+ W  QRKT ++EF +   RQ 
Sbjct: 88  PKNIEHMLKTRFDNYPKGPTWQAVFHDFLGQGIFNSDGDTWLFQRKTAALEFTTRTLRQA 147

Query: 153 TVESLHELVHSRLLPILHRHFVEHLVPIDLQDILLRLTFDNVCMIAFGVEPGCLNPNLPV 212
               ++  +  R  PIL      +  P+DLQD++LRLTFDN+C +AFG +     P LP 
Sbjct: 148 MGRWVNRGIKLRFCPILET-AQNNYEPVDLQDLILRLTFDNICGLAFGKDTRTCAPGLPE 207

Query: 213 IPFAHAFEVATEATVLRFVTPQFLWKTMRHFNLGTEKSLKFALKEVNEFAMNVIEKRRRE 272
             FA AF+ ATEA++ RF+ P+FLW+  +   LG E SL  +L E++ +   VI  R++E
Sbjct: 208 NGFASAFDRATEASLQRFILPEFLWRLKKWLGLGLEVSLSRSLGEIDGYLDAVINTRKQE 267

Query: 273 KVNETE-----RSDLLSVFTGLKDENGEPYSDKFLKDICVNFILAGRDTSSVASSWFFWL 332
            +++ E       DLLS F   KD++   YS+ FL+ + +NFILAGRDTSSVA SWFFWL
Sbjct: 268 LLSQRESGVQRHDDLLSRFMKKKDQS---YSETFLRHVALNFILAGRDTSSVALSWFFWL 327

Query: 333 IDRNPEVEERIVEKICRIL-KKRGEAAGESSGGNVTFEVEEIKKMEYLQAALSEALRLFP 392
           I  +P VE++IV +IC +L + RG     SS      E +E+ ++ YL+AALSE LRL+P
Sbjct: 328 ITTHPTVEDKIVREICSVLIETRG--TDVSSWTAEPLEFDEVDRLVYLKAALSETLRLYP 387

Query: 393 SVPVDHK---EDDVLPNGTELKKGTKLVYAIYAMGRTEAIWGKDCYEFKPERWLR--DSR 452
           SVP D K    DD+LP+GT +  G+ + Y+IYA GR ++ WG+DC EFKPERW+   D +
Sbjct: 388 SVPEDSKHVVNDDILPDGTFVPAGSSVTYSIYAAGRMKSTWGEDCLEFKPERWISPDDGK 447

Query: 453 FTAVLGKRFCILPNEVHFCL 457
           F      RF         CL
Sbjct: 448 FVNHDQYRFVAFNAGPRICL 460

BLAST of CSPI03G29340 vs. ExPASy TrEMBL
Match: A0A0A0LCB0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G681140 PE=3 SV=1)

HSP 1 Score: 884.8 bits (2285), Expect = 1.6e-253
Identity = 432/437 (98.86%), Postives = 433/437 (99.08%), Query Frame = 0

Query: 1   MESLLLTRHSNVRDIHSNFYFYSNKIIEAKKKQGLPVWPVVGMLPSLVTGLRSNLYEWIT 60
           MESLLLTRHSNVRDIHSNFYFYSNKIIEAKKKQGLPVWPVVGMLPSLVTGLRSNLYEWIT
Sbjct: 1   MESLLLTRHSNVRDIHSNFYFYSNKIIEAKKKQGLPVWPVVGMLPSLVTGLRSNLYEWIT 60

Query: 61  EVLCRQNGTFRFKGPWFSSLNWVVTSDPRNIEHILKTEFLKYPKGSFFRETVHDLLGDGI 120
           EVLCRQNGTFRFKGPWFSSLNWVVTSDPRNIEHILKTEFLKYPKGSFFRETVHDLLGDGI
Sbjct: 61  EVLCRQNGTFRFKGPWFSSLNWVVTSDPRNIEHILKTEFLKYPKGSFFRETVHDLLGDGI 120

Query: 121 FNADGEIWHKQRKTTSIEFHSAKFRQLTVESLHELVHSRLLPILHRHFVEHLVPIDLQDI 180
           FNADGEIWHKQRKTTSIEFHSAKFRQLTVESLHELVHSRLLPILHRHFVEHLVPIDLQDI
Sbjct: 121 FNADGEIWHKQRKTTSIEFHSAKFRQLTVESLHELVHSRLLPILHRHFVEHLVPIDLQDI 180

Query: 181 LLRLTFDNVCMIAFGVEPGCLNPNLPVIPFAHAFEVATEATVLRFVTPQFLWKTMRHFNL 240
           LLRLTFDNVCMIAFGVEPGCLNPNLPVIPFAHAFEVATEATVLRFVTPQFLWKTMRHFNL
Sbjct: 181 LLRLTFDNVCMIAFGVEPGCLNPNLPVIPFAHAFEVATEATVLRFVTPQFLWKTMRHFNL 240

Query: 241 GTEKSLKFALKEVNEFAMNVIEKRRREKVNETERSDLLSVFTGLKDENGEPYSDKFLKDI 300
           GTEKSLKFALKEVNEFAMNVIEKRRREKVNETERSDLLSVFTGLKDENGEPYSDKFLKDI
Sbjct: 241 GTEKSLKFALKEVNEFAMNVIEKRRREKVNETERSDLLSVFTGLKDENGEPYSDKFLKDI 300

Query: 301 CVNFILAGRDTSSVASSWFFWLIDRNPEVEERIVEKICRILKKRGEAAGESSGGNVTFEV 360
           CVNFILAGRDTSSVASSWFFWLIDRNPEVEERIVE+ICRILKKRGEAAGESSGGNV FEV
Sbjct: 301 CVNFILAGRDTSSVASSWFFWLIDRNPEVEERIVEEICRILKKRGEAAGESSGGNVKFEV 360

Query: 361 EEIKKMEYLQAALSEALRLFPSVPVDHKE---DDVLPNGTELKKGTKLVYAIYAMGRTEA 420
           EEIKKMEYLQAALSEALRLFPSVPVDHKE   DDVLPNGTELKKGTKLVYAIYAMGRTEA
Sbjct: 361 EEIKKMEYLQAALSEALRLFPSVPVDHKEVIQDDVLPNGTELKKGTKLVYAIYAMGRTEA 420

Query: 421 IWGKDCYEFKPERWLRD 435
           IWGKDCYEFKPERWLRD
Sbjct: 421 IWGKDCYEFKPERWLRD 437

BLAST of CSPI03G29340 vs. ExPASy TrEMBL
Match: A0A1S3CG42 (cytochrome P450 86B1-like OS=Cucumis melo OX=3656 GN=LOC103500347 PE=3 SV=1)

HSP 1 Score: 748.0 bits (1930), Expect = 2.3e-212
Identity = 370/441 (83.90%), Postives = 390/441 (88.44%), Query Frame = 0

Query: 19  FYFYSNKIIEAKKKQGLPVWPVVGMLPSLVTGLRSNLYEWITEVLCRQNGTFRFKGPWFS 78
           F F + + +  ++KQGLPVWPVVGMLPSLVTGLRSNLYEWITEVL  QNGTFRFKGPWFS
Sbjct: 41  FIFTAIRSLRQRRKQGLPVWPVVGMLPSLVTGLRSNLYEWITEVLYHQNGTFRFKGPWFS 100

Query: 79  SLNWVVTSDPRNIEHILKTEFLKYPKGSFFRETVHDLLGDGIFNADGEIWHKQRKTTSIE 138
           SL+ VVTSDPRNIEHILKTEFLKYPKGSFFRETVHDLLGDGIFNADGEIWHKQRKTTSIE
Sbjct: 101 SLHCVVTSDPRNIEHILKTEFLKYPKGSFFRETVHDLLGDGIFNADGEIWHKQRKTTSIE 160

Query: 139 FHSAKFRQLTVESLHELVHSRLLPILHRHFVEHLVPIDLQDILLRLTFDNVCMIAFGVEP 198
           FHSAKFRQLTVESLH LVHSRLLPI  RHFVEHLVPIDLQDILLRLTFDNVCMIAFGV+P
Sbjct: 161 FHSAKFRQLTVESLHVLVHSRLLPIFDRHFVEHLVPIDLQDILLRLTFDNVCMIAFGVDP 220

Query: 199 GCLNPNLPVIPFAHAFEVATEATVLRFVTPQFLWKTMRHFNLGTEKSLKFALKEVNEFAM 258
           GCL+PNLP IPFA+AFEVATEATVLRFVTPQF+WKTMR+FNLGTEKSLKFALKEVNEFAM
Sbjct: 221 GCLSPNLPAIPFANAFEVATEATVLRFVTPQFIWKTMRYFNLGTEKSLKFALKEVNEFAM 280

Query: 259 NVIEKRRREKVNETERSDLLSVFTGLKDENGEPYSDKFLKDICVNFILAGRDTSSVASSW 318
           NVIEKRRREKVNE ERSDLLSVF GLKDENGEPYSDKFLKDICVNFILAGRDTSSVA SW
Sbjct: 281 NVIEKRRREKVNEIERSDLLSVFMGLKDENGEPYSDKFLKDICVNFILAGRDTSSVALSW 340

Query: 319 FFWLIDRNPEVEERIVEKICRILKKRGEAAGESSGGNVTFEVEEIKKMEYLQAALSEALR 378
           FFWLID NPEVEERI+E+I RILKKRGEA   SS GNV FEVEEIKKMEYL+AA+SEALR
Sbjct: 341 FFWLIDGNPEVEERILEEIFRILKKRGEAGEGSSAGNVRFEVEEIKKMEYLEAAISEALR 400

Query: 379 LFPSVPVDHKE---DDVLPNGTELKKGTKLVYAIYAMGRTEAIWGKDCYEFKPERWLRDS 438
           LFPSVPVDHKE   DDVLP+GT+LKKGTK++YAIYAM R EA       EFKPERWL+D 
Sbjct: 401 LFPSVPVDHKEVIKDDVLPDGTQLKKGTKVIYAIYAMARMEAXXXXXXXEFKPERWLKDG 460

Query: 439 RFTAVLGKRFCILPNEVHFCL 457
           RF      RF         CL
Sbjct: 461 RFMTESAYRFTPFNGGPRLCL 481

BLAST of CSPI03G29340 vs. ExPASy TrEMBL
Match: A0A5A7UF06 (Cytochrome P450 86B1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1856G00050 PE=3 SV=1)

HSP 1 Score: 676.8 bits (1745), Expect = 6.5e-191
Identity = 333/371 (89.76%), Postives = 348/371 (93.80%), Query Frame = 0

Query: 19  FYFYSNKIIEAKKKQGLPVWPVVGMLPSLVTGLRSNLYEWITEVLCRQNGTFRFKGPWFS 78
           F F + + +  ++KQGLPVWPVVGMLPSLVTGLRSNLYEWITEVL  QNGTFRFKGPWFS
Sbjct: 41  FIFTAIRSLRQRRKQGLPVWPVVGMLPSLVTGLRSNLYEWITEVLYHQNGTFRFKGPWFS 100

Query: 79  SLNWVVTSDPRNIEHILKTEFLKYPKGSFFRETVHDLLGDGIFNADGEIWHKQRKTTSIE 138
           SL+ VVTSDPRNIEHILKTEFLKYPKGSFFRETVHDLLGDGIFNADGEIWHKQRKTTSIE
Sbjct: 101 SLHCVVTSDPRNIEHILKTEFLKYPKGSFFRETVHDLLGDGIFNADGEIWHKQRKTTSIE 160

Query: 139 FHSAKFRQLTVESLHELVHSRLLPILHRHFVEHLVPIDLQDILLRLTFDNVCMIAFGVEP 198
           FHSAKFRQLTVESLH LVHSRLLPI  RHFVEHLVPIDLQDILLRLTFDNVCMIAFGV+P
Sbjct: 161 FHSAKFRQLTVESLHVLVHSRLLPIFDRHFVEHLVPIDLQDILLRLTFDNVCMIAFGVDP 220

Query: 199 GCLNPNLPVIPFAHAFEVATEATVLRFVTPQFLWKTMRHFNLGTEKSLKFALKEVNEFAM 258
           GCL+PNLP IPFA+AFEVATEATVLRFVTPQF+WKTMR+FNLGTEKSLKFALKEVNEFAM
Sbjct: 221 GCLSPNLPAIPFANAFEVATEATVLRFVTPQFIWKTMRYFNLGTEKSLKFALKEVNEFAM 280

Query: 259 NVIEKRRREKVNETERSDLLSVFTGLKDENGEPYSDKFLKDICVNFILAGRDTSSVASSW 318
           NVIEKRRREKVNE ERSDLLSVF GLKDENGEPYSDKFLKDICVNFILAGRDTSSVA SW
Sbjct: 281 NVIEKRRREKVNEIERSDLLSVFMGLKDENGEPYSDKFLKDICVNFILAGRDTSSVALSW 340

Query: 319 FFWLIDRNPEVEERIVEKICRILKKRGEAAGESSGGNVTFEVEEIKKMEYLQAALSEALR 378
           FFWLID NPEVEERI+E+I RILKKRGEA   SS GNV FEVEEIKKMEYL+AA+SEALR
Sbjct: 341 FFWLIDGNPEVEERILEEIFRILKKRGEAGEGSSAGNVRFEVEEIKKMEYLEAAISEALR 400

Query: 379 LFPSVPVDHKE 390
           LFPSVPVDHKE
Sbjct: 401 LFPSVPVDHKE 411

BLAST of CSPI03G29340 vs. ExPASy TrEMBL
Match: A0A438FZ12 (Cytochrome P450 86B1 OS=Vitis vinifera OX=29760 GN=CYP86B1_3 PE=3 SV=1)

HSP 1 Score: 611.3 bits (1575), Expect = 3.3e-171
Identity = 298/437 (68.19%), Postives = 350/437 (80.09%), Query Frame = 0

Query: 27  IEAKKKQGLPVWPVVGMLPSLVTGLRSNLYEWITEVLCRQNGTFRFKGPWFSSLNWVVTS 86
           +  +K+ GLPVWP +GMLPSLV GLR+++YEWI+ VLCRQNGTF FKGPWFSSLN VVTS
Sbjct: 61  LRQRKRHGLPVWPFLGMLPSLVGGLRTDMYEWISGVLCRQNGTFVFKGPWFSSLNCVVTS 120

Query: 87  DPRNIEHILKTEFLKYPKGSFFRETVHDLLGDGIFNADGEIWHKQRKTTSIEFHSAKFRQ 146
           DPRNIEH+LK +F  +PKG++FR TV DLLGDGIF+AD E W +QRKT SIEFHSAKFR+
Sbjct: 121 DPRNIEHLLKGKFSNFPKGAYFRNTVRDLLGDGIFSADDETWQRQRKTASIEFHSAKFRK 180

Query: 147 LTVESLHELVHSRLLPILHRHFVEHLVPIDLQDILLRLTFDNVCMIAFGVEPGCLNPNLP 206
           LT+ESL  LVHSRLLP+L    V++   IDLQD+LLRLTFDNVCMIAFGV+PGCL   LP
Sbjct: 181 LTIESLLVLVHSRLLPVL-EDSVKNSASIDLQDVLLRLTFDNVCMIAFGVDPGCLRLGLP 240

Query: 207 VIPFAHAFEVATEATVLRFVTPQFLWKTMRHFNLGTEKSLKFALKEVNEFAMNVIEKRRR 266
            IPFA AFE ATEATVLRFVTP  +WK MRH N+GTEK+LK ++  V++FA  VI  R++
Sbjct: 241 EIPFARAFEDATEATVLRFVTPTCIWKAMRHLNIGTEKNLKISIMGVDKFANEVITTRKK 300

Query: 267 EKV----NETERSDLLSVFTGLKDENGEPYSDKFLKDICVNFILAGRDTSSVASSWFFWL 326
           E      ++ +RSDLL++F GLKDENG+P+SDKFL+DICVNFILAGRDTSSVA SWFFWL
Sbjct: 301 ELSLQCDDKNQRSDLLTIFMGLKDENGQPFSDKFLRDICVNFILAGRDTSSVALSWFFWL 360

Query: 327 IDRNPEVEERIVEKICRILKKRGEAAGESSGGNVTFEVEEIKKMEYLQAALSEALRLFPS 386
           +DRNP VEERI+ +ICR++   GE  GE  G  +TF+ EE+KKMEYLQAALSEALRL+PS
Sbjct: 361 LDRNPAVEERIMAEICRMV---GERKGEEEGDGLTFKAEEVKKMEYLQAALSEALRLYPS 420

Query: 387 VPVDHK---EDDVLPNGTELKKGTKLVYAIYAMGRTEAIWGKDCYEFKPERWLRDSRFTA 446
           VPVDHK   EDDV P+GT LKKGTK+VYAIYAMGR E IWGKDC EFKPERWLRD RF +
Sbjct: 421 VPVDHKEVIEDDVFPDGTVLKKGTKVVYAIYAMGRMEGIWGKDCREFKPERWLRDGRFMS 480

Query: 447 VLGKRFCILPNEVHFCL 457
               +F         CL
Sbjct: 481 ESAYKFTAFNGGPRLCL 493

BLAST of CSPI03G29340 vs. ExPASy TrEMBL
Match: F6HF53 (Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VIT_01s0011g02060 PE=3 SV=1)

HSP 1 Score: 608.6 bits (1568), Expect = 2.2e-170
Identity = 297/437 (67.96%), Postives = 349/437 (79.86%), Query Frame = 0

Query: 27  IEAKKKQGLPVWPVVGMLPSLVTGLRSNLYEWITEVLCRQNGTFRFKGPWFSSLNWVVTS 86
           +  +K+ GLPVWP +GMLPSLV GLR+++YEWI+ VLCR+NGTF FKGPWFSSLN VVTS
Sbjct: 49  LRQRKRHGLPVWPFLGMLPSLVGGLRTDMYEWISGVLCRRNGTFVFKGPWFSSLNCVVTS 108

Query: 87  DPRNIEHILKTEFLKYPKGSFFRETVHDLLGDGIFNADGEIWHKQRKTTSIEFHSAKFRQ 146
           DPRNIEH+LK +F  +PKG++FR TV DLLGDGIF+AD E W +QRKT SIEFHSAKFR+
Sbjct: 109 DPRNIEHLLKGKFSNFPKGAYFRNTVRDLLGDGIFSADDETWQRQRKTASIEFHSAKFRK 168

Query: 147 LTVESLHELVHSRLLPILHRHFVEHLVPIDLQDILLRLTFDNVCMIAFGVEPGCLNPNLP 206
           LT+ESL  LVHSRLLP+L    V++   IDLQD+LLRLTFDNVCMIAFGV+PGCL   LP
Sbjct: 169 LTIESLLVLVHSRLLPVL-EDSVKNSASIDLQDVLLRLTFDNVCMIAFGVDPGCLRLGLP 228

Query: 207 VIPFAHAFEVATEATVLRFVTPQFLWKTMRHFNLGTEKSLKFALKEVNEFAMNVIEKRRR 266
            IPFA AFE ATEATVLRFVTP  +WK MRH N+GTEK+LK ++  V++FA  VI  R++
Sbjct: 229 EIPFARAFEDATEATVLRFVTPTCIWKAMRHLNIGTEKNLKISIMGVDKFANEVITTRKK 288

Query: 267 EKV----NETERSDLLSVFTGLKDENGEPYSDKFLKDICVNFILAGRDTSSVASSWFFWL 326
           E      ++ +RSDLLS+F GLKDENG+P+SDKFL+DICVNFILAGRDTSSVA SWFFWL
Sbjct: 289 ELSLQCDDKNQRSDLLSIFMGLKDENGQPFSDKFLRDICVNFILAGRDTSSVALSWFFWL 348

Query: 327 IDRNPEVEERIVEKICRILKKRGEAAGESSGGNVTFEVEEIKKMEYLQAALSEALRLFPS 386
           +DRNP VEERI+ +ICR++   GE  GE  G  + F+ EE+KKMEYLQAALSEALRL+PS
Sbjct: 349 LDRNPAVEERIMAEICRMV---GERKGEEEGDGLIFKAEEVKKMEYLQAALSEALRLYPS 408

Query: 387 VPVDHK---EDDVLPNGTELKKGTKLVYAIYAMGRTEAIWGKDCYEFKPERWLRDSRFTA 446
           VPVDHK   EDDV P+GT LKKGTK+VYAIYAMGR E IWGKDC EFKPERWLRD RF +
Sbjct: 409 VPVDHKEVIEDDVFPDGTVLKKGTKVVYAIYAMGRMEGIWGKDCREFKPERWLRDGRFMS 468

Query: 447 VLGKRFCILPNEVHFCL 457
               +F         CL
Sbjct: 469 ESAYKFTAFNGGPRLCL 481

BLAST of CSPI03G29340 vs. NCBI nr
Match: XP_011651758.2 (cytochrome P450 86B1 [Cucumis sativus] >KGN58549.1 hypothetical protein Csa_000732 [Cucumis sativus])

HSP 1 Score: 884.8 bits (2285), Expect = 3.2e-253
Identity = 432/437 (98.86%), Postives = 433/437 (99.08%), Query Frame = 0

Query: 1   MESLLLTRHSNVRDIHSNFYFYSNKIIEAKKKQGLPVWPVVGMLPSLVTGLRSNLYEWIT 60
           MESLLLTRHSNVRDIHSNFYFYSNKIIEAKKKQGLPVWPVVGMLPSLVTGLRSNLYEWIT
Sbjct: 1   MESLLLTRHSNVRDIHSNFYFYSNKIIEAKKKQGLPVWPVVGMLPSLVTGLRSNLYEWIT 60

Query: 61  EVLCRQNGTFRFKGPWFSSLNWVVTSDPRNIEHILKTEFLKYPKGSFFRETVHDLLGDGI 120
           EVLCRQNGTFRFKGPWFSSLNWVVTSDPRNIEHILKTEFLKYPKGSFFRETVHDLLGDGI
Sbjct: 61  EVLCRQNGTFRFKGPWFSSLNWVVTSDPRNIEHILKTEFLKYPKGSFFRETVHDLLGDGI 120

Query: 121 FNADGEIWHKQRKTTSIEFHSAKFRQLTVESLHELVHSRLLPILHRHFVEHLVPIDLQDI 180
           FNADGEIWHKQRKTTSIEFHSAKFRQLTVESLHELVHSRLLPILHRHFVEHLVPIDLQDI
Sbjct: 121 FNADGEIWHKQRKTTSIEFHSAKFRQLTVESLHELVHSRLLPILHRHFVEHLVPIDLQDI 180

Query: 181 LLRLTFDNVCMIAFGVEPGCLNPNLPVIPFAHAFEVATEATVLRFVTPQFLWKTMRHFNL 240
           LLRLTFDNVCMIAFGVEPGCLNPNLPVIPFAHAFEVATEATVLRFVTPQFLWKTMRHFNL
Sbjct: 181 LLRLTFDNVCMIAFGVEPGCLNPNLPVIPFAHAFEVATEATVLRFVTPQFLWKTMRHFNL 240

Query: 241 GTEKSLKFALKEVNEFAMNVIEKRRREKVNETERSDLLSVFTGLKDENGEPYSDKFLKDI 300
           GTEKSLKFALKEVNEFAMNVIEKRRREKVNETERSDLLSVFTGLKDENGEPYSDKFLKDI
Sbjct: 241 GTEKSLKFALKEVNEFAMNVIEKRRREKVNETERSDLLSVFTGLKDENGEPYSDKFLKDI 300

Query: 301 CVNFILAGRDTSSVASSWFFWLIDRNPEVEERIVEKICRILKKRGEAAGESSGGNVTFEV 360
           CVNFILAGRDTSSVASSWFFWLIDRNPEVEERIVE+ICRILKKRGEAAGESSGGNV FEV
Sbjct: 301 CVNFILAGRDTSSVASSWFFWLIDRNPEVEERIVEEICRILKKRGEAAGESSGGNVKFEV 360

Query: 361 EEIKKMEYLQAALSEALRLFPSVPVDHKE---DDVLPNGTELKKGTKLVYAIYAMGRTEA 420
           EEIKKMEYLQAALSEALRLFPSVPVDHKE   DDVLPNGTELKKGTKLVYAIYAMGRTEA
Sbjct: 361 EEIKKMEYLQAALSEALRLFPSVPVDHKEVIQDDVLPNGTELKKGTKLVYAIYAMGRTEA 420

Query: 421 IWGKDCYEFKPERWLRD 435
           IWGKDCYEFKPERWLRD
Sbjct: 421 IWGKDCYEFKPERWLRD 437

BLAST of CSPI03G29340 vs. NCBI nr
Match: XP_038903356.1 (LOW QUALITY PROTEIN: cytochrome P450 86B1-like [Benincasa hispida])

HSP 1 Score: 752.7 bits (1942), Expect = 1.9e-213
Identity = 370/441 (83.90%), Postives = 394/441 (89.34%), Query Frame = 0

Query: 21  FYSNKIIEAKKKQGLPVWPVVGMLPSLVTGLRSNLYEWITEVLCRQNGTFRFKGPWFSSL 80
           F + K +  ++KQGLPVWPV+ MLPSLVTGLR NLYEWITEVL  QNGTFRFKGPWFSSL
Sbjct: 43  FTAIKSLRQRRKQGLPVWPVIRMLPSLVTGLRGNLYEWITEVLYHQNGTFRFKGPWFSSL 102

Query: 81  NWVVTSDPRNIEHILKTEFLKYPKGSFFRETVHDLLGDGIFNADGEIWHKQRKTTSIEFH 140
           N VVTSDPRNIEHILKTEF KYPKGSFFRETV DLLGDGIFNADGEIWH QRKT SIEFH
Sbjct: 103 NCVVTSDPRNIEHILKTEFPKYPKGSFFRETVRDLLGDGIFNADGEIWHCQRKTASIEFH 162

Query: 141 SAKFRQLTVESLHELVHSRLLPILHRHFVEHLVPIDLQDILLRLTFDNVCMIAFGVEPGC 200
           SAKFRQLTVESLHELVHSRLLPI+H+HFVE LVPIDLQDILLRLTFDNVCMIAFGV+PGC
Sbjct: 163 SAKFRQLTVESLHELVHSRLLPIVHKHFVEQLVPIDLQDILLRLTFDNVCMIAFGVDPGC 222

Query: 201 LNPNLPVIPFAHAFEVATEATVLRFVTPQFLWKTMRHFNLGTEKSLKFALKEVNEFAMNV 260
           L+PNLP IPFA+AFEVATEATVLRFVTP+F+WKTMR+FNLGTEKSLKFALKEVN+FA +V
Sbjct: 223 LSPNLPAIPFANAFEVATEATVLRFVTPKFIWKTMRYFNLGTEKSLKFALKEVNQFAKSV 282

Query: 261 IEKRRREKVNETERSDLLSVFTGLKDENGEPYSDKFLKDICVNFILAGRDTSSVASSWFF 320
           IEKR+RE VNE ERSDLLSVF GLKDENGEPYS+KFLKDICVNFILAGRDTSSVA SWFF
Sbjct: 283 IEKRKRENVNENERSDLLSVFLGLKDENGEPYSEKFLKDICVNFILAGRDTSSVALSWFF 342

Query: 321 WLIDRNPEVEERIVEKICRILKKRGE--AAGESSGGNVTFEVEEIKKMEYLQAALSEALR 380
           WLIDRNPEVEERI+E+ICRIL+KRGE  AAGESS G+V FEVEEIKKMEYLQAALSEALR
Sbjct: 343 WLIDRNPEVEERILEEICRILEKRGEAAAAGESSAGDVAFEVEEIKKMEYLQAALSEALR 402

Query: 381 LFPSVPVDHKE---DDVLPNGTELKKGTKLVYAIYAMGRTEAIWGKDCYEFKPERWLRDS 440
           L PSVPVDHKE   DDVLP+G ELKKGTK++YAIYAMGR EAIWGKDC EFKPERWLRD 
Sbjct: 403 LXPSVPVDHKEVIKDDVLPDGRELKKGTKVIYAIYAMGRMEAIWGKDCNEFKPERWLRDG 462

Query: 441 RFTAVLGKRFCILPNEVHFCL 457
           RF A    RF         CL
Sbjct: 463 RFVAESAYRFTAFNGGPRLCL 483

BLAST of CSPI03G29340 vs. NCBI nr
Match: XP_008461842.1 (PREDICTED: cytochrome P450 86B1-like [Cucumis melo])

HSP 1 Score: 748.0 bits (1930), Expect = 4.7e-212
Identity = 370/441 (83.90%), Postives = 390/441 (88.44%), Query Frame = 0

Query: 19  FYFYSNKIIEAKKKQGLPVWPVVGMLPSLVTGLRSNLYEWITEVLCRQNGTFRFKGPWFS 78
           F F + + +  ++KQGLPVWPVVGMLPSLVTGLRSNLYEWITEVL  QNGTFRFKGPWFS
Sbjct: 41  FIFTAIRSLRQRRKQGLPVWPVVGMLPSLVTGLRSNLYEWITEVLYHQNGTFRFKGPWFS 100

Query: 79  SLNWVVTSDPRNIEHILKTEFLKYPKGSFFRETVHDLLGDGIFNADGEIWHKQRKTTSIE 138
           SL+ VVTSDPRNIEHILKTEFLKYPKGSFFRETVHDLLGDGIFNADGEIWHKQRKTTSIE
Sbjct: 101 SLHCVVTSDPRNIEHILKTEFLKYPKGSFFRETVHDLLGDGIFNADGEIWHKQRKTTSIE 160

Query: 139 FHSAKFRQLTVESLHELVHSRLLPILHRHFVEHLVPIDLQDILLRLTFDNVCMIAFGVEP 198
           FHSAKFRQLTVESLH LVHSRLLPI  RHFVEHLVPIDLQDILLRLTFDNVCMIAFGV+P
Sbjct: 161 FHSAKFRQLTVESLHVLVHSRLLPIFDRHFVEHLVPIDLQDILLRLTFDNVCMIAFGVDP 220

Query: 199 GCLNPNLPVIPFAHAFEVATEATVLRFVTPQFLWKTMRHFNLGTEKSLKFALKEVNEFAM 258
           GCL+PNLP IPFA+AFEVATEATVLRFVTPQF+WKTMR+FNLGTEKSLKFALKEVNEFAM
Sbjct: 221 GCLSPNLPAIPFANAFEVATEATVLRFVTPQFIWKTMRYFNLGTEKSLKFALKEVNEFAM 280

Query: 259 NVIEKRRREKVNETERSDLLSVFTGLKDENGEPYSDKFLKDICVNFILAGRDTSSVASSW 318
           NVIEKRRREKVNE ERSDLLSVF GLKDENGEPYSDKFLKDICVNFILAGRDTSSVA SW
Sbjct: 281 NVIEKRRREKVNEIERSDLLSVFMGLKDENGEPYSDKFLKDICVNFILAGRDTSSVALSW 340

Query: 319 FFWLIDRNPEVEERIVEKICRILKKRGEAAGESSGGNVTFEVEEIKKMEYLQAALSEALR 378
           FFWLID NPEVEERI+E+I RILKKRGEA   SS GNV FEVEEIKKMEYL+AA+SEALR
Sbjct: 341 FFWLIDGNPEVEERILEEIFRILKKRGEAGEGSSAGNVRFEVEEIKKMEYLEAAISEALR 400

Query: 379 LFPSVPVDHKE---DDVLPNGTELKKGTKLVYAIYAMGRTEAIWGKDCYEFKPERWLRDS 438
           LFPSVPVDHKE   DDVLP+GT+LKKGTK++YAIYAM R EA       EFKPERWL+D 
Sbjct: 401 LFPSVPVDHKEVIKDDVLPDGTQLKKGTKVIYAIYAMARMEAXXXXXXXEFKPERWLKDG 460

Query: 439 RFTAVLGKRFCILPNEVHFCL 457
           RF      RF         CL
Sbjct: 461 RFMTESAYRFTPFNGGPRLCL 481

BLAST of CSPI03G29340 vs. NCBI nr
Match: KAA0052776.1 (cytochrome P450 86B1-like [Cucumis melo var. makuwa] >TYK04179.1 cytochrome P450 86B1-like [Cucumis melo var. makuwa])

HSP 1 Score: 676.8 bits (1745), Expect = 1.3e-190
Identity = 333/371 (89.76%), Postives = 348/371 (93.80%), Query Frame = 0

Query: 19  FYFYSNKIIEAKKKQGLPVWPVVGMLPSLVTGLRSNLYEWITEVLCRQNGTFRFKGPWFS 78
           F F + + +  ++KQGLPVWPVVGMLPSLVTGLRSNLYEWITEVL  QNGTFRFKGPWFS
Sbjct: 41  FIFTAIRSLRQRRKQGLPVWPVVGMLPSLVTGLRSNLYEWITEVLYHQNGTFRFKGPWFS 100

Query: 79  SLNWVVTSDPRNIEHILKTEFLKYPKGSFFRETVHDLLGDGIFNADGEIWHKQRKTTSIE 138
           SL+ VVTSDPRNIEHILKTEFLKYPKGSFFRETVHDLLGDGIFNADGEIWHKQRKTTSIE
Sbjct: 101 SLHCVVTSDPRNIEHILKTEFLKYPKGSFFRETVHDLLGDGIFNADGEIWHKQRKTTSIE 160

Query: 139 FHSAKFRQLTVESLHELVHSRLLPILHRHFVEHLVPIDLQDILLRLTFDNVCMIAFGVEP 198
           FHSAKFRQLTVESLH LVHSRLLPI  RHFVEHLVPIDLQDILLRLTFDNVCMIAFGV+P
Sbjct: 161 FHSAKFRQLTVESLHVLVHSRLLPIFDRHFVEHLVPIDLQDILLRLTFDNVCMIAFGVDP 220

Query: 199 GCLNPNLPVIPFAHAFEVATEATVLRFVTPQFLWKTMRHFNLGTEKSLKFALKEVNEFAM 258
           GCL+PNLP IPFA+AFEVATEATVLRFVTPQF+WKTMR+FNLGTEKSLKFALKEVNEFAM
Sbjct: 221 GCLSPNLPAIPFANAFEVATEATVLRFVTPQFIWKTMRYFNLGTEKSLKFALKEVNEFAM 280

Query: 259 NVIEKRRREKVNETERSDLLSVFTGLKDENGEPYSDKFLKDICVNFILAGRDTSSVASSW 318
           NVIEKRRREKVNE ERSDLLSVF GLKDENGEPYSDKFLKDICVNFILAGRDTSSVA SW
Sbjct: 281 NVIEKRRREKVNEIERSDLLSVFMGLKDENGEPYSDKFLKDICVNFILAGRDTSSVALSW 340

Query: 319 FFWLIDRNPEVEERIVEKICRILKKRGEAAGESSGGNVTFEVEEIKKMEYLQAALSEALR 378
           FFWLID NPEVEERI+E+I RILKKRGEA   SS GNV FEVEEIKKMEYL+AA+SEALR
Sbjct: 341 FFWLIDGNPEVEERILEEIFRILKKRGEAGEGSSAGNVRFEVEEIKKMEYLEAAISEALR 400

Query: 379 LFPSVPVDHKE 390
           LFPSVPVDHKE
Sbjct: 401 LFPSVPVDHKE 411

BLAST of CSPI03G29340 vs. NCBI nr
Match: RVW65197.1 (Cytochrome P450 86B1 [Vitis vinifera])

HSP 1 Score: 611.3 bits (1575), Expect = 6.9e-171
Identity = 298/437 (68.19%), Postives = 350/437 (80.09%), Query Frame = 0

Query: 27  IEAKKKQGLPVWPVVGMLPSLVTGLRSNLYEWITEVLCRQNGTFRFKGPWFSSLNWVVTS 86
           +  +K+ GLPVWP +GMLPSLV GLR+++YEWI+ VLCRQNGTF FKGPWFSSLN VVTS
Sbjct: 61  LRQRKRHGLPVWPFLGMLPSLVGGLRTDMYEWISGVLCRQNGTFVFKGPWFSSLNCVVTS 120

Query: 87  DPRNIEHILKTEFLKYPKGSFFRETVHDLLGDGIFNADGEIWHKQRKTTSIEFHSAKFRQ 146
           DPRNIEH+LK +F  +PKG++FR TV DLLGDGIF+AD E W +QRKT SIEFHSAKFR+
Sbjct: 121 DPRNIEHLLKGKFSNFPKGAYFRNTVRDLLGDGIFSADDETWQRQRKTASIEFHSAKFRK 180

Query: 147 LTVESLHELVHSRLLPILHRHFVEHLVPIDLQDILLRLTFDNVCMIAFGVEPGCLNPNLP 206
           LT+ESL  LVHSRLLP+L    V++   IDLQD+LLRLTFDNVCMIAFGV+PGCL   LP
Sbjct: 181 LTIESLLVLVHSRLLPVL-EDSVKNSASIDLQDVLLRLTFDNVCMIAFGVDPGCLRLGLP 240

Query: 207 VIPFAHAFEVATEATVLRFVTPQFLWKTMRHFNLGTEKSLKFALKEVNEFAMNVIEKRRR 266
            IPFA AFE ATEATVLRFVTP  +WK MRH N+GTEK+LK ++  V++FA  VI  R++
Sbjct: 241 EIPFARAFEDATEATVLRFVTPTCIWKAMRHLNIGTEKNLKISIMGVDKFANEVITTRKK 300

Query: 267 EKV----NETERSDLLSVFTGLKDENGEPYSDKFLKDICVNFILAGRDTSSVASSWFFWL 326
           E      ++ +RSDLL++F GLKDENG+P+SDKFL+DICVNFILAGRDTSSVA SWFFWL
Sbjct: 301 ELSLQCDDKNQRSDLLTIFMGLKDENGQPFSDKFLRDICVNFILAGRDTSSVALSWFFWL 360

Query: 327 IDRNPEVEERIVEKICRILKKRGEAAGESSGGNVTFEVEEIKKMEYLQAALSEALRLFPS 386
           +DRNP VEERI+ +ICR++   GE  GE  G  +TF+ EE+KKMEYLQAALSEALRL+PS
Sbjct: 361 LDRNPAVEERIMAEICRMV---GERKGEEEGDGLTFKAEEVKKMEYLQAALSEALRLYPS 420

Query: 387 VPVDHK---EDDVLPNGTELKKGTKLVYAIYAMGRTEAIWGKDCYEFKPERWLRDSRFTA 446
           VPVDHK   EDDV P+GT LKKGTK+VYAIYAMGR E IWGKDC EFKPERWLRD RF +
Sbjct: 421 VPVDHKEVIEDDVFPDGTVLKKGTKVVYAIYAMGRMEGIWGKDCREFKPERWLRDGRFMS 480

Query: 447 VLGKRFCILPNEVHFCL 457
               +F         CL
Sbjct: 481 ESAYKFTAFNGGPRLCL 493

BLAST of CSPI03G29340 vs. TAIR 10
Match: AT5G23190.1 (cytochrome P450, family 86, subfamily B, polypeptide 1 )

HSP 1 Score: 589.3 bits (1518), Expect = 2.6e-168
Identity = 287/445 (64.49%), Postives = 345/445 (77.53%), Query Frame = 0

Query: 19  FYFYSNKIIEAKKKQGLPVWPVVGMLPSLVTGLRSNLYEWITEVLCRQNGTFRFKGPWFS 78
           F F +   +  KK QGLPVWP +GMLPSL  GLR N+YEW+++VLC QNGTF+F+GPWFS
Sbjct: 46  FVFVAIHALRQKKYQGLPVWPFLGMLPSLAFGLRGNIYEWLSDVLCLQNGTFQFRGPWFS 105

Query: 79  SLNWVVTSDPRNIEHILKTEFLKYPKGSFFRETVHDLLGDGIFNADGEIWHKQRKTTSIE 138
           SLN  +T DPRN+EH+LK  F  +PKGS+FR+ + DLLGDGIFNAD E W +QRKT SIE
Sbjct: 106 SLNSTITCDPRNVEHLLKNRFSVFPKGSYFRDNLRDLLGDGIFNADDETWQRQRKTASIE 165

Query: 139 FHSAKFRQLTVESLHELVHSRLLPILHRHFVEHLVPIDLQDILLRLTFDNVCMIAFGVEP 198
           FHSAKFRQLT +SL ELVH RLLP+L    V+   PIDLQD+LLRLTFDNVCMIAFGV+P
Sbjct: 166 FHSAKFRQLTTQSLFELVHKRLLPVLETS-VKSSSPIDLQDVLLRLTFDNVCMIAFGVDP 225

Query: 199 GCLNPNLPVIPFAHAFEVATEATVLRFVTPQFLWKTMRHFNLGTEKSLKFALKEVNEFAM 258
           GCL P+ PVIPFA AFE ATEA V+RFV P  +WK MR+ ++GTEK LK ++K V++FA 
Sbjct: 226 GCLGPDQPVIPFAKAFEDATEAAVVRFVMPTCVWKFMRYLDIGTEKKLKESIKGVDDFAD 285

Query: 259 NVIEKRRREKVNE---TERSDLLSVFTGLKDENGEPYSDKFLKDICVNFILAGRDTSSVA 318
            VI  R++E   E   T+RSDLL+VF GL+DE GE +SDKFL+DICVNFILAGRDTSSVA
Sbjct: 286 EVIRTRKKELSLEGETTKRSDLLTVFMGLRDEKGESFSDKFLRDICVNFILAGRDTSSVA 345

Query: 319 SSWFFWLIDRNPEVEERIVEKICRILKKRGE-AAGESSGGNVTFEVEEIKKMEYLQAALS 378
            SWFFWL+++NPEVEE+I+ ++C+IL++R +    E S     F  EEIKKM+YLQAALS
Sbjct: 346 LSWFFWLLEKNPEVEEKIMVEMCKILRQRDDHGNAEKSDYEPVFGPEEIKKMDYLQAALS 405

Query: 379 EALRLFPSVPVDHK---EDDVLPNGTELKKGTKLVYAIYAMGRTEAIWGKDCYEFKPERW 438
           EALRL+PSVPVDHK   EDDV P+GT LKKG K++YAIYAMGR EAIWGKDC EF+PERW
Sbjct: 406 EALRLYPSVPVDHKEVQEDDVFPDGTMLKKGDKVIYAIYAMGRMEAIWGKDCLEFRPERW 465

Query: 439 LRDSRFTAVLGKRFCILPNEVHFCL 457
           LRD RF +    +F         CL
Sbjct: 466 LRDGRFMSESAYKFTAFNGGPRLCL 489

BLAST of CSPI03G29340 vs. TAIR 10
Match: AT5G08250.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 579.3 bits (1492), Expect = 2.7e-165
Identity = 282/430 (65.58%), Postives = 336/430 (78.14%), Query Frame = 0

Query: 35  LPVWPVVGMLPSLVTGLRSNLYEWITEVLCRQNGTFRFKGPWFSSLNWVVTSDPRNIEHI 94
           +PVWP+VGMLPSL++ +RSN+YEW+++VL  QNGTFRF+GPWFS+LN VVT DPRN+EH+
Sbjct: 1   MPVWPLVGMLPSLISAVRSNIYEWLSDVLISQNGTFRFRGPWFSTLNCVVTCDPRNVEHL 60

Query: 95  LKTEFLKYPKGSFFRETVHDLLGDGIFNADGEIWHKQRKTTSIEFHSAKFRQLTVESLHE 154
           LKT F  YPKGS+FRET+ DLLGDGIFN D   W +QRK  S+EFHSAKFRQLT +SLHE
Sbjct: 61  LKTRFSIYPKGSYFRETMQDLLGDGIFNTDDGTWQRQRKAASVEFHSAKFRQLTSQSLHE 120

Query: 155 LVHSRLLPILHRHFVEHLVPIDLQDILLRLTFDNVCMIAFGVEPGCLNPNLPVIPFAHAF 214
           LVH+RLLP+L     E    IDLQDILLRLTFDNVCMIAFGV+PGCL+P LP IPFA AF
Sbjct: 121 LVHNRLLPVL-----ETSGKIDLQDILLRLTFDNVCMIAFGVDPGCLSPKLPEIPFAKAF 180

Query: 215 EVATEATVLRFVTPQFLWKTMRHFNLGTEKSLKFALKEVNEFAMNVIEKRRREKVNETE- 274
           E ATEATV+RFV P+F+WK MR  NLGTEK LK ++  V++FA  VI  R++E   ETE 
Sbjct: 181 EDATEATVVRFVMPKFVWKLMRSLNLGTEKKLKESINGVDDFAEEVIRTRKKEMSLETEI 240

Query: 275 --RSDLLSVFTGLKDENGEPYSDKFLKDICVNFILAGRDTSSVASSWFFWLIDRNPEVEE 334
             R DLL++F GL+DENG+ +SDKFL+DICVNFILAGRDTSSVA SWFFWLI++NPEVEE
Sbjct: 241 AKRPDLLTIFMGLRDENGQKFSDKFLRDICVNFILAGRDTSSVALSWFFWLIEKNPEVEE 300

Query: 335 RIVEKICRILKKRGEAAGESSGGNV--TFEVEEIKKMEYLQAALSEALRLFPSVPVDHK- 394
           +I+  IC+IL++R +             F  EEIKKM+YLQAALSE LRL+PSVPVDHK 
Sbjct: 301 KIMMGICKILEQRVDHGDTKKNMEYEPVFRPEEIKKMDYLQAALSETLRLYPSVPVDHKE 360

Query: 395 --EDDVLPNGTELKKGTKLVYAIYAMGRTEAIWGKDCYEFKPERWLRDSRFTAVLGKRFC 454
             EDDV P+GT+LKKG K++YAIYAMGR E IWGKDC EFKPERWLRD R+ +    +F 
Sbjct: 361 VLEDDVFPDGTKLKKGEKVIYAIYAMGRMETIWGKDCREFKPERWLRDGRYMSESAYKFT 420

Query: 455 ILPNEVHFCL 457
                   CL
Sbjct: 421 AFNGGPRLCL 425

BLAST of CSPI03G29340 vs. TAIR 10
Match: AT1G24540.1 (cytochrome P450, family 86, subfamily C, polypeptide 1 )

HSP 1 Score: 391.7 bits (1005), Expect = 8.0e-109
Identity = 194/420 (46.19%), Postives = 275/420 (65.48%), Query Frame = 0

Query: 32  KQGLPVWPVVGMLPSLVTGLRSNLYEWITEVLCRQNGTFRFKGPWFSSLNWVVTSDPRNI 91
           K G  +WPV+G++P L    + +L+ W T  + R  GTF ++G WF     ++T+DP N+
Sbjct: 40  KGGPVMWPVLGIIPMLALN-KHDLFTWCTRCVVRSGGTFHYRGIWFGGAYGIMTADPANV 99

Query: 92  EHILKTEFLKYPKGSFFRETVHDLLGDGIFNADGEIWHKQRKTTSIEFHSAKFRQLTVES 151
           EHILKT F  YPKG+F+RE   DLL DGIFNAD E+W ++R+    E HS++F + T  +
Sbjct: 100 EHILKTNFKNYPKGAFYRERFRDLLEDGIFNADDELWKEERRVAKTEMHSSRFLEHTFTT 159

Query: 152 LHELVHSRLLPILHRHFVEHLVPIDLQDILLRLTFDNVCMIAFGVEPGCLNPNLPVIPFA 211
           + +LV  +L+P++        V  DLQD+LLR TFDN+C+ AFGV PG L   LP IPFA
Sbjct: 160 MRDLVDQKLVPLMENLSTSKRV-FDLQDLLLRFTFDNICISAFGVYPGSLETGLPEIPFA 219

Query: 212 HAFEVATEATVLRFVTPQFLWKTMRHFNLGTEKSLKFALKEVNEFAMNVIEKRR---REK 271
            AFE ATE T+ RF+ P F+WK MR   +G E+ L  A++ V+ FA   + +RR   R+ 
Sbjct: 220 KAFEDATEYTLARFLIPPFVWKPMRFLGIGYERKLNNAVRIVHAFANKTVRERRNKMRKL 279

Query: 272 VNETERSDLLSVFTGLKDE------NGEPYSDKFLKDICVNFILAGRDTSSVASSWFFWL 331
            N  + +DLLS     + E       G  +SDK+ ++ C +FI+AGRDT+SVA  WFFWL
Sbjct: 280 GNLNDYADLLSRLMQREYEKEEDTTRGNYFSDKYFREFCTSFIIAGRDTTSVALVWFFWL 339

Query: 332 IDRNPEVEERIVEKICRILKKRGEAAGESSGGNVTFEVEEIKKMEYLQAALSEALRLFPS 391
           + ++PEVE+RI+ +I  I +K      E       FE E+ ++M YLQAAL+E+LRL+PS
Sbjct: 340 VQKHPEVEKRILREIREIKRKLTTQETEDQ-----FEAEDFREMVYLQAALTESLRLYPS 399

Query: 392 VPVDHK---EDDVLPNGTELKKGTKLVYAIYAMGRTEAIWGKDCYEFKPERWLRDSRFTA 440
           VP++ K   EDDVLP+GT +KKG ++ Y++Y+MGR E+IWGKD  EFKPERW+++ R  +
Sbjct: 400 VPMEMKQALEDDVLPDGTRVKKGARIHYSVYSMGRIESIWGKDWEEFKPERWIKEGRIVS 452

BLAST of CSPI03G29340 vs. TAIR 10
Match: AT1G13140.1 (cytochrome P450, family 86, subfamily C, polypeptide 3 )

HSP 1 Score: 375.2 bits (962), Expect = 7.8e-104
Identity = 185/417 (44.36%), Postives = 270/417 (64.75%), Query Frame = 0

Query: 31  KKQGLPVWPVVGMLPSLVTGLRSNLYEWITEVLCRQNGTFRFKGPWFSSLNWVVTSDPRN 90
           KK G  +WPV G+ P      R+++Y W T  L +  GTF + G W       VT  P N
Sbjct: 42  KKLGPTIWPVFGITPEFFFH-RNDVYGWATRCLKKCRGTFLYNGIWLGGSYGAVTCVPAN 101

Query: 91  IEHILKTEFLKYPKGSFFRETVHDLLGDGIFNADGEIWHKQRKTTSIEFHSAKFRQLTVE 150
           +E++LKT F  +PKG+FF+E  +DLL DGIFNAD E W +QR+    E HS +F + + +
Sbjct: 102 VEYMLKTNFKNFPKGAFFKERFNDLLEDGIFNADAESWKEQRRIIITEMHSTRFVEHSFQ 161

Query: 151 SLHELVHSRLLPILHRHFVEHLVPIDLQDILLRLTFDNVCMIAFGVEPGCLNPNLPVIPF 210
           +  +LV  +LL ++   F       DLQD+LLRLTFDN+C+   G +PG L+ +LP++PF
Sbjct: 162 TTQDLVRKKLLKVM-ESFARSQEAFDLQDVLLRLTFDNICIAGLGDDPGTLDSDLPLVPF 221

Query: 211 AHAFEVATEATVLRFVTPQFLWKTMRHFNLGTEKSLKFALKEVNEFAMNVIEKR---RRE 270
           A AFE ATE+T+ RF+ P F+WK ++ F++G EK L+ A+  V+EF   ++  R    +E
Sbjct: 222 AQAFEEATESTMFRFMIPPFIWKPLKFFDIGYEKGLRKAVDVVHEFVDKMVVDRICKLKE 281

Query: 271 KVNETERSDLLSVFTGLKD----ENGEPYSDKFLKDICVNFILAGRDTSSVASSWFFWLI 330
           +     RSD+LS    ++     +  +P + KF +  C +FILAGRDTSSVA +WFFW+I
Sbjct: 282 EGTLGNRSDVLSRIIEIESHKTTDEKDPSTIKFFRQFCTSFILAGRDTSSVALTWFFWVI 341

Query: 331 DRNPEVEERIVEKICRILKKRGEAAGESSGGNVTFEVEEIKKMEYLQAALSEALRLFPSV 390
            ++PEVE +I+ +I  IL++RG++   +S     F V+E+  M YLQAALSE +RL+P +
Sbjct: 342 QKHPEVENKIIREISEILRQRGDS--PTSKNESLFTVKELNDMVYLQAALSETMRLYPPI 401

Query: 391 PVDHK---EDDVLPNGTELKKGTKLVYAIYAMGRTEAIWGKDCYEFKPERWLRDSRF 438
           P++ K   EDDV P+GT ++KG+++ +A YAMGR E+IWGKDC  FKPERW++   F
Sbjct: 402 PMEMKQAIEDDVFPDGTFIRKGSRVYFATYAMGRMESIWGKDCESFKPERWIQSGNF 454

BLAST of CSPI03G29340 vs. TAIR 10
Match: AT3G26125.1 (cytochrome P450, family 86, subfamily C, polypeptide 2 )

HSP 1 Score: 360.9 bits (925), Expect = 1.5e-99
Identity = 190/443 (42.89%), Postives = 280/443 (63.21%), Query Frame = 0

Query: 33  QGLPV-WPVVGMLPSLVTGLRSNLYEWITEVLCRQNGTFRFKGPWFSSLNWVVTSDPRNI 92
           +G PV WPV G+ P     +  +++ W+T  L +  GTF ++G WFS     +TS P NI
Sbjct: 43  KGEPVQWPVFGITPEFFLHVH-DMFGWVTRCLTKSRGTFPYQGIWFSGSYGAMTSVPANI 102

Query: 93  EHILKTEFLKYPKGSFFRETVHDLLGDGIFNADGEIWHKQRKTTSIEFHSAKFRQLTVES 152
           E++LKT F  +PKG F++E   DLL DGIFNAD E W +QR+    E HS +F   + ++
Sbjct: 103 EYMLKTNFKNFPKGEFYKERFRDLLEDGIFNADDESWKEQRRIIITEMHSTRFVDHSFQT 162

Query: 153 LHELVHSRLLPILHRHFVEHLVPIDLQDILLRLTFDNVCMIAFGVEPGCLNPNLPVIPFA 212
             +L+  +LL ++   F +     DLQ+ILLRLTFDN+C+   G +PG L+ +LP +PFA
Sbjct: 163 TRDLIERKLLKVM-ESFSKSQEAFDLQEILLRLTFDNICIAGLGDDPGTLDDDLPHVPFA 222

Query: 213 HAFEVATEATVLRFVTPQFLWKTMRHFNLGTEKSLKFALKEVNEFAMNVIEKR----RRE 272
            AFE ATE+T+ RF+ P F+WK M+ F++G EK L+ A++ V+ F   ++ +R    + +
Sbjct: 223 KAFEEATESTLFRFMIPPFVWKPMKFFDIGYEKGLREAVETVHNFIDKMVVERIAMLKDQ 282

Query: 273 KVNETERSDLLSVFTGLK-----DENGEPYSDKFLKDICVNFILAGRDTSSVASSWFFWL 332
                 +SD+LS    ++     DEN + ++ KF +  C +FILAGRDTSSVA +WFFWL
Sbjct: 283 GTLANSKSDVLSRLIQIESHKRGDEN-DRFTAKFFRQFCTSFILAGRDTSSVALTWFFWL 342

Query: 333 IDRNPEVEERIVEKICRILKKRG------EAAGESSGGNVTFEVEEIKKMEYLQAALSEA 392
           I ++PEVE +I+ +I  IL +R       +  GE       F V+E+  M YLQAALSE+
Sbjct: 343 ITKHPEVETKILHEIREILNQREKNKYKLDETGEKESSR-HFTVKELNDMVYLQAALSES 402

Query: 393 LRLFPSVPVDHK---EDDVLPNGTELKKGTKLVYAIYAMGRTEAIWGKDCYEFKPERWLR 448
           LRL+P +P++ K   E+DV P+GT L+KG+++ +++YAMGR E+IWGKDC  FKPERW++
Sbjct: 403 LRLYPPIPMEMKQATEEDVFPDGTFLRKGSRVYFSVYAMGRMESIWGKDCEMFKPERWIQ 462

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FMY13.7e-16764.49Cytochrome P450 86B1 OS=Arabidopsis thaliana OX=3702 GN=CYP86B1 PE=2 SV=1[more]
Q9CAD62.8e-9846.28Cytochrome P450 86A7 OS=Arabidopsis thaliana OX=3702 GN=CYP86A7 PE=2 SV=1[more]
B3RFJ64.0e-9746.75Cytochrome P450 86A22 OS=Petunia hybrida OX=4102 GN=CYP86A22 PE=1 SV=1[more]
Q9LMM11.2e-9344.93Cytochrome P450 86A4 OS=Arabidopsis thaliana OX=3702 GN=CYP86A4 PE=1 SV=1[more]
O230661.6e-9343.86Cytochrome P450 86A2 OS=Arabidopsis thaliana OX=3702 GN=CYP86A2 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LCB01.6e-25398.86Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G681140 PE=3 SV=1[more]
A0A1S3CG422.3e-21283.90cytochrome P450 86B1-like OS=Cucumis melo OX=3656 GN=LOC103500347 PE=3 SV=1[more]
A0A5A7UF066.5e-19189.76Cytochrome P450 86B1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... [more]
A0A438FZ123.3e-17168.19Cytochrome P450 86B1 OS=Vitis vinifera OX=29760 GN=CYP86B1_3 PE=3 SV=1[more]
F6HF532.2e-17067.96Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VIT_01s0011g02060 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
XP_011651758.23.2e-25398.86cytochrome P450 86B1 [Cucumis sativus] >KGN58549.1 hypothetical protein Csa_0007... [more]
XP_038903356.11.9e-21383.90LOW QUALITY PROTEIN: cytochrome P450 86B1-like [Benincasa hispida][more]
XP_008461842.14.7e-21283.90PREDICTED: cytochrome P450 86B1-like [Cucumis melo][more]
KAA0052776.11.3e-19089.76cytochrome P450 86B1-like [Cucumis melo var. makuwa] >TYK04179.1 cytochrome P450... [more]
RVW65197.16.9e-17168.19Cytochrome P450 86B1 [Vitis vinifera][more]
Match NameE-valueIdentityDescription
AT5G23190.12.6e-16864.49cytochrome P450, family 86, subfamily B, polypeptide 1 [more]
AT5G08250.12.7e-16565.58Cytochrome P450 superfamily protein [more]
AT1G24540.18.0e-10946.19cytochrome P450, family 86, subfamily C, polypeptide 1 [more]
AT1G13140.17.8e-10444.36cytochrome P450, family 86, subfamily C, polypeptide 3 [more]
AT3G26125.11.5e-9942.89cytochrome P450, family 86, subfamily C, polypeptide 2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 27..447
e-value: 6.1E-78
score: 264.4
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 34..446
IPR001128Cytochrome P450PFAMPF00067p450coord: 34..436
e-value: 1.6E-52
score: 178.8
NoneNo IPR availablePANTHERPTHR24296:SF177CYTOCHROME P450 86B1-LIKEcoord: 26..456
NoneNo IPR availablePANTHERPTHR24296CYTOCHROME P450coord: 26..456

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI03G29340.1CSPI03G29340.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen