CSPI03G29190 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI03G29190
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionPectin lyase-like superfamily protein
LocationChr3: 26794131 .. 26796626 (+)
RNA-Seq ExpressionCSPI03G29190
SyntenyCSPI03G29190
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACATTCATAATCTTCTTCTTTTGCCTTTTGTTTCCTGTTCATGGCCGTTGGCATAACCATGCAAAGAAACACAAACAACATTCTCACCATCATGACCATTTCACCATTTCCCAACCTCCTTCTCCTTCCCCTTCGTCAGAACCAGCCGGGAGTCCTCCGGAAGACAATGGAGGGCCGCCATCGTCGTCCATCAACTCCACTAGCATTTTTGATGTACGTCATTTTGGCGCCATTGGAGATGGCACTGCGGACGACACGGCCGCGTTCAAGATGGCGTGGGACACAGCATGTCAAAGGAATGACGATGACAACAACGACGACTATGGAGTTATTCTCGTTCCCTATGGCTTCTCCTTTATGATTCAGTCGACTATTTTCACAGGACCGTGCAAAAATGGCGTTGTGTTTCAAGTTGATGGCACTCTTATGGCACCTGACGGGCCTGACGCTTGGCCGAAGGGTTATAGTGCTCACCAGTGGTTGGTTTTTTATCGAGTTTATAACATGTCACTTCAAGGAAATGGCCTTATTGATGGTCGAGGCCAAAAATGGTGGGATCTTCCTTGCAAACCTCACAAGGTAAGGGACAACAACGATCATAGTCTAGAAATCTTTTTACTTCATTTCGAGGTCGTTTTGAAAGCTAACTTGAGGTTTTGATTCTATTTTTAAAGGGGAAAACAATGACAGGACCTTGTGACAGCCCAATTGTAAGTTAAAATTCAGTTCTCTATAATAAAACTTTTTTTTTTTCCTTTTAAAGTTTGCAAATATATATGAAAAAGTCAAGTGAAAAGAGTTAAAAGTGATTTTTTTTGACAGGCTATAAGATTTTTCATGAGCTCAGAGTTGAGAGTTGAAGGAGTTAGAATCAAAGACAGTCCTCAATTCCATTTTAGGTTTGACAACTGTAAAGATGTTTATATTGATTCTCTTCACATAACATCTCCTGCTTTGAGCCCAAACACTGATGGAATCCACATTGAGAATACACATGGTGTTCAGATTTTCAATTCAATAGTTGCCAATGGTTAGTCCTCATACACATATAACATTGTTTCCTAATTATCTTCTCTTTGCATTTTAATTTCTAAAAATGACAGAATAATTTTTCAAAGTAAAAACCACAGTAGAAATGAGTAGGTGTATCACTATTGGATAATGTTCTCCTTTCTAGTTTTTTATTACAAAATTGTGGTTTTGTTTTGAAAAAATATATCTAAATGCATTTTTTAGACTAAAACAATAAACTATTTTTTTTTCTTTTAAAAAATTGTGTAAACATTTGGTGTGTTTCAAAGCTAAAGAATTAGAGACATCACAATAAATATATATGTTAAACTCATGACATTTCTTAAGAAATTAGAAACAAGAAAAAAGAATAAAATGTTAATTATAATATATATTCTTGTATTTTGATTTCTCGTTTATCATTTTCTATTTTTGTTGCTTGTTTTTACTTTTTAATAGTGAAAAACATAATAATTGTATTTGGTAACTATTTGTTCAGTATCTTATTTATTAAAAATATATTTTAAATAACGTTGAACATACGAACACTTTTAAGTTGTTTTTATTTTTAAATATTTATTAGTGATTTTACAATCACATATGGTTAAAGATTATTTCATAATTTTTTATGTCGGAATAACGTTGTACTTAAATAAAGAACAAAATTAAATATGTTTTTAAGTAGTTGAAAGGAGGAAACAAAAAATGACAAGCGTAAACGTTACCAAATAATTAATTTCCACAGATTTAAAATAGAACTTATGAAGCAACGACTCCTACCAAATTTTTTTCACGCAGGTGATGATTGTGTTTCAATTGGATCGGGTTCATTTAACGTAGAAATAAGAAACATTACATGTGGACCAGGTCATGGAATTAGGTAAACAAATCATGAAATCTTATTAAAATTTCTTCTATAATTAATTCTTATATAACATGTAATTTCTTTTTATACTCACAACCCTAGTTCTTCATGTCAGCATTGGTAGTCTAGGGAATCACAACTCACGAGCATGCGTATCGAACATAACAGTTCGGGACTCAATAATTAAGGTCTCAGACAATGGTGTTCGAATCAAGACATGGCAAGGTGGATTTGGTACCGTAAGAGGAGTAAGTTTCAACAATATTAACATGGATAACGTTAGAAATCCTATAATAATTGACCAATTCTATTGCCTCAACAAAGGATGTCTGAATCAAACTTCAGCTGTGGTTGTATCAGATATAAGCTATATTGACATCAAAGGAACTTATGATATTAGAAGCCCACCAATGCATTTTGGTTGTAGTGATTCTATGCCTTGTATGAACCTAACACTTGCCAATATTGAGCTTTTTCCTTCTAGAGGTGACATCTTGTTGGACCCCTTTTGTTGGAATGCTTATGGGAATTTGCAAACCCTAACAATACCACCAATTTCTTGCTTGCTCGAAACCTCCTCAAGGTCTTTCTTGGAAAGTGAAAAGGATTATTGTTAA

mRNA sequence

ATGACATTCATAATCTTCTTCTTTTGCCTTTTGTTTCCTGTTCATGGCCGTTGGCATAACCATGCAAAGAAACACAAACAACATTCTCACCATCATGACCATTTCACCATTTCCCAACCTCCTTCTCCTTCCCCTTCGTCAGAACCAGCCGGGAGTCCTCCGGAAGACAATGGAGGGCCGCCATCGTCGTCCATCAACTCCACTAGCATTTTTGATGTACGTCATTTTGGCGCCATTGGAGATGGCACTGCGGACGACACGGCCGCGTTCAAGATGGCGTGGGACACAGCATGTCAAAGGAATGACGATGACAACAACGACGACTATGGAGTTATTCTCGTTCCCTATGGCTTCTCCTTTATGATTCAGTCGACTATTTTCACAGGACCGTGCAAAAATGGCGTTGTGTTTCAAGTTGATGGCACTCTTATGGCACCTGACGGGCCTGACGCTTGGCCGAAGGGTTATAGTGCTCACCAGTGGTTGGTTTTTTATCGAGTTTATAACATGTCACTTCAAGGAAATGGCCTTATTGATGGTCGAGGCCAAAAATGGTGGGATCTTCCTTGCAAACCTCACAAGGGGAAAACAATGACAGGACCTTGTGACAGCCCAATTGCTATAAGATTTTTCATGAGCTCAGAGTTGAGAGTTGAAGGAGTTAGAATCAAAGACAGTCCTCAATTCCATTTTAGGTTTGACAACTGTAAAGATGTTTATATTGATTCTCTTCACATAACATCTCCTGCTTTGAGCCCAAACACTGATGGAATCCACATTGAGAATACACATGGTGTTCAGATTTTCAATTCAATAGTTGCCAATGGTGATGATTGTGTTTCAATTGGATCGGGTTCATTTAACGTAGAAATAAGAAACATTACATGTGGACCAGGTCATGGAATTAGCATTGGTAGTCTAGGGAATCACAACTCACGAGCATGCGTATCGAACATAACAGTTCGGGACTCAATAATTAAGGTCTCAGACAATGGTGTTCGAATCAAGACATGGCAAGGTGGATTTGGTACCGTAAGAGGAGTAAGTTTCAACAATATTAACATGGATAACGTTAGAAATCCTATAATAATTGACCAATTCTATTGCCTCAACAAAGGATGTCTGAATCAAACTTCAGCTGTGGTTGTATCAGATATAAGCTATATTGACATCAAAGGAACTTATGATATTAGAAGCCCACCAATGCATTTTGGTTGTAGTGATTCTATGCCTTGTATGAACCTAACACTTGCCAATATTGAGCTTTTTCCTTCTAGAGGTGACATCTTGTTGGACCCCTTTTGTTGGAATGCTTATGGGAATTTGCAAACCCTAACAATACCACCAATTTCTTGCTTGCTCGAAACCTCCTCAAGGTCTTTCTTGGAAAGTGAAAAGGATTATTGTTAA

Coding sequence (CDS)

ATGACATTCATAATCTTCTTCTTTTGCCTTTTGTTTCCTGTTCATGGCCGTTGGCATAACCATGCAAAGAAACACAAACAACATTCTCACCATCATGACCATTTCACCATTTCCCAACCTCCTTCTCCTTCCCCTTCGTCAGAACCAGCCGGGAGTCCTCCGGAAGACAATGGAGGGCCGCCATCGTCGTCCATCAACTCCACTAGCATTTTTGATGTACGTCATTTTGGCGCCATTGGAGATGGCACTGCGGACGACACGGCCGCGTTCAAGATGGCGTGGGACACAGCATGTCAAAGGAATGACGATGACAACAACGACGACTATGGAGTTATTCTCGTTCCCTATGGCTTCTCCTTTATGATTCAGTCGACTATTTTCACAGGACCGTGCAAAAATGGCGTTGTGTTTCAAGTTGATGGCACTCTTATGGCACCTGACGGGCCTGACGCTTGGCCGAAGGGTTATAGTGCTCACCAGTGGTTGGTTTTTTATCGAGTTTATAACATGTCACTTCAAGGAAATGGCCTTATTGATGGTCGAGGCCAAAAATGGTGGGATCTTCCTTGCAAACCTCACAAGGGGAAAACAATGACAGGACCTTGTGACAGCCCAATTGCTATAAGATTTTTCATGAGCTCAGAGTTGAGAGTTGAAGGAGTTAGAATCAAAGACAGTCCTCAATTCCATTTTAGGTTTGACAACTGTAAAGATGTTTATATTGATTCTCTTCACATAACATCTCCTGCTTTGAGCCCAAACACTGATGGAATCCACATTGAGAATACACATGGTGTTCAGATTTTCAATTCAATAGTTGCCAATGGTGATGATTGTGTTTCAATTGGATCGGGTTCATTTAACGTAGAAATAAGAAACATTACATGTGGACCAGGTCATGGAATTAGCATTGGTAGTCTAGGGAATCACAACTCACGAGCATGCGTATCGAACATAACAGTTCGGGACTCAATAATTAAGGTCTCAGACAATGGTGTTCGAATCAAGACATGGCAAGGTGGATTTGGTACCGTAAGAGGAGTAAGTTTCAACAATATTAACATGGATAACGTTAGAAATCCTATAATAATTGACCAATTCTATTGCCTCAACAAAGGATGTCTGAATCAAACTTCAGCTGTGGTTGTATCAGATATAAGCTATATTGACATCAAAGGAACTTATGATATTAGAAGCCCACCAATGCATTTTGGTTGTAGTGATTCTATGCCTTGTATGAACCTAACACTTGCCAATATTGAGCTTTTTCCTTCTAGAGGTGACATCTTGTTGGACCCCTTTTGTTGGAATGCTTATGGGAATTTGCAAACCCTAACAATACCACCAATTTCTTGCTTGCTCGAAACCTCCTCAAGGTCTTTCTTGGAAAGTGAAAAGGATTATTGTTAA

Protein sequence

MTFIIFFFCLLFPVHGRWHNHAKKHKQHSHHHDHFTISQPPSPSPSSEPAGSPPEDNGGPPSSSINSTSIFDVRHFGAIGDGTADDTAAFKMAWDTACQRNDDDNNDDYGVILVPYGFSFMIQSTIFTGPCKNGVVFQVDGTLMAPDGPDAWPKGYSAHQWLVFYRVYNMSLQGNGLIDGRGQKWWDLPCKPHKGKTMTGPCDSPIAIRFFMSSELRVEGVRIKDSPQFHFRFDNCKDVYIDSLHITSPALSPNTDGIHIENTHGVQIFNSIVANGDDCVSIGSGSFNVEIRNITCGPGHGISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGFGTVRGVSFNNINMDNVRNPIIIDQFYCLNKGCLNQTSAVVVSDISYIDIKGTYDIRSPPMHFGCSDSMPCMNLTLANIELFPSRGDILLDPFCWNAYGNLQTLTIPPISCLLETSSRSFLESEKDYC*
Homology
BLAST of CSPI03G29190 vs. ExPASy Swiss-Prot
Match: Q949Z1 (Polygalacturonase At1g48100 OS=Arabidopsis thaliana OX=3702 GN=At1g48100 PE=2 SV=1)

HSP 1 Score: 522.3 bits (1344), Expect = 5.6e-147
Identity = 248/464 (53.45%), Postives = 327/464 (70.47%), Query Frame = 0

Query: 4   IIFFFCLLFPVHGRWHNHAKKHKQHSHHHDH-FTISQPPSPSPSSEPAGSP------PED 63
           I+  + +   + G + N A+  + H H   H  T   PP P P  E A  P      P  
Sbjct: 7   ILVIYIISTVLFGHFTNVARA-RYHYHKGRHGVTHPLPPPPPPPLETANPPDQVPSDPYP 66

Query: 64  NGGPPSSSINSTSIFDVRHFGAIGDGTADDTAAFKMAWDTACQRNDDDNNDDYGVILVPY 123
           +  P     +S  +FDV  FGA+GDG+ DDTAAF+ AW  AC         + GV+L P 
Sbjct: 67  SPDPAPGDSDSGCVFDVTSFGAVGDGSCDDTAAFQDAWKAACA-------VESGVVLAPE 126

Query: 124 GFSFMIQSTIFTGPCKNGVVFQVDGTLMAPDGPDAWPKGYSAHQWLVFYRVYNMSLQGNG 183
           G  F I STIF+GPCK G+VFQ+DG LM PDGP+ WP+  + +QWLVFYR+   +  G G
Sbjct: 127 GGVFKITSTIFSGPCKPGLVFQLDGVLMPPDGPEEWPEKDNKNQWLVFYRLDGFTFSGKG 186

Query: 184 LIDGRGQKWWDLPCKPHK---GKTMTGPCDSPIAIRFFMSSELRVEGVRIKDSPQFHFRF 243
            ++G GQKWWDLPCKPH+   G + +GPC SP  IRFFMS+ + V+G+RI++SPQFH +F
Sbjct: 187 TVEGNGQKWWDLPCKPHRGPDGSSSSGPCASPTMIRFFMSNNIEVKGLRIQNSPQFHMKF 246

Query: 244 DNCKDVYIDSLHITSPALSPNTDGIHIENTHGVQIFNSIVANGDDCVSIGSGSFNVEIRN 303
           D C+ V I+ + I+SP LSPNTDGIH+ NT  V I+NS+V+NGDDC+SIG+G  +V+I+ 
Sbjct: 247 DGCQGVLINEIQISSPKLSPNTDGIHLGNTRSVGIYNSVVSNGDDCISIGTGCSDVDIQG 306

Query: 304 ITCGPGHGISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGFGTVRGVSFNNI 363
           +TCGP HGISIGSLG HNS+ACVSNITVR+++I+ SDNG+R+KTWQGG G+V  + F NI
Sbjct: 307 VTCGPSHGISIGSLGVHNSQACVSNITVRNTVIRDSDNGLRVKTWQGGTGSVSNLLFENI 366

Query: 364 NMDNVRNPIIIDQFYCLNKGCLNQTSAVVVSDISYIDIKGTYDIRSPPMHFGCSDSMPCM 423
            M+NV N II+DQ+YC +K C N+TSAV V D+ Y +IKGTYD+RSPP+HF CSD++ C 
Sbjct: 367 QMENVLNCIIVDQYYCQSKDCRNETSAVKVFDVEYRNIKGTYDVRSPPIHFACSDTVACT 426

Query: 424 NLTLANIELFPSRGDILLDPFCWNAYGNLQTLTIPPISCLLETS 458
           N+T++ +EL P  G+++ DPFCWNAYG  +TLTIPPI CLL+ S
Sbjct: 427 NITMSEVELLPEEGELVDDPFCWNAYGKQETLTIPPIDCLLDGS 462

BLAST of CSPI03G29190 vs. ExPASy Swiss-Prot
Match: Q94AJ5 (Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g80170 PE=1 SV=1)

HSP 1 Score: 341.7 bits (875), Expect = 1.4e-92
Identity = 175/404 (43.32%), Postives = 240/404 (59.41%), Query Frame = 0

Query: 62  SSSINSTSIFDVRHFGAIGDGTADDTAAFKMAWDTACQRNDDDNNDDYGVILVPYGFSFM 121
           S+   S  +  V +FGA G+G  DDT AF  AW TAC             ILVP  ++ +
Sbjct: 44  STRPRSERLLHVGNFGAKGNGVTDDTKAFADAWKTACSSKVKTR------ILVPENYTCL 103

Query: 122 IQSTIFTGPCKNGVVFQVDGTLMAPDGPDAWPKGYSAHQWLVFYRVYNMSLQGNGLIDGR 181
           ++    +GPCK  +  Q+ GT++AP+ PD W +G +  +WL F+ +  ++++G G ++G 
Sbjct: 104 LRPIDLSGPCKARLTLQISGTIIAPNDPDVW-EGLNRRKWLYFHGLSRLTVEGGGTVNGM 163

Query: 182 GQKWWDLPCKPHKGKTMTGPC-DSPIAIRFFMSSELRVEGVRIKDSPQFHFRFDNCKDVY 241
           GQ+WW+  CK +     + PC  +P A+ F     +RVE + + DS Q H    +C+ V 
Sbjct: 164 GQEWWERSCKHNH----SNPCRGAPTALTFHKCKNMRVENLNVIDSQQMHIALTSCRRVT 223

Query: 242 IDSLHITSPALSPNTDGIHIENTHGVQIFNSIVANGDDCVSIGSGSFNVEIRNITCGPGH 301
           I  L + +PA SPNTDGIHI  + G+ I N+ V+ GDDC+SI   S  + I NI CGPGH
Sbjct: 224 ISGLKVIAPATSPNTDGIHISVSRGIVIDNTTVSTGDDCISIVKNSTQISISNIICGPGH 283

Query: 302 GISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGFGTVRGVSFNNINMDNVRN 361
           GISIGSLG   S   V +ITV  +II  + NGVRIKTWQGG G V  + F NI M+NV N
Sbjct: 284 GISIGSLGKSKSWEEVRDITVDTAIISDTANGVRIKTWQGGSGLVSKIIFRNIKMNNVSN 343

Query: 362 PIIIDQFYC-LNKGCLNQTSAVVVSDISYIDIKGTYDIRSPPMHFGCSDSMPCMNLTLAN 421
           PIIIDQ+YC   K C NQTSA+ + +IS++ ++GT       +   CSDS PC N+ L +
Sbjct: 344 PIIIDQYYCDSRKPCANQTSAISIENISFVHVRGT-SASKEAIKISCSDSSPCRNILLQD 403

Query: 422 IELFPSRGDILLDPFCWNAYGNLQTLTIPPISCLLETSSRSFLE 464
           I+L PS GD   + FCW AYG+      PP  CL  +   SFLE
Sbjct: 404 IDLEPSNGDGFTESFCWEAYGSSSGQVYPP-PCL--SDDTSFLE 432

BLAST of CSPI03G29190 vs. ExPASy Swiss-Prot
Match: P43212 (Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1)

HSP 1 Score: 305.8 bits (782), Expect = 8.2e-82
Identity = 161/405 (39.75%), Postives = 233/405 (57.53%), Query Frame = 0

Query: 66  NSTSIFDVRHFGAIGDGTADDTAAFKMAWDTACQRNDDDNNDDYGVILVPYGFSFMIQST 125
           ++ +IF+V  +GA+GDG  D T AF  AW  AC++          ++LVP    F++ + 
Sbjct: 54  DAINIFNVEKYGAVGDGKHDCTEAFSTAWQAACKKPS-------AMLLVPGNKKFVVNNL 113

Query: 126 IFTGPCKNGVVFQVDGTLMAPDGPDAWPKGYSAHQWLVFYRVYNMSLQGNGLIDGRGQKW 185
            F GPC+    F+VDG + A   P +W        WL F ++   +L G G+IDG+G++W
Sbjct: 114 FFNGPCQPHFTFKVDGIIAAYQNPASWKNN---RIWLQFAKLTGFTLMGKGVIDGQGKQW 173

Query: 186 WDLPCKPHKGKTMTGPCDSPIAIRFFMSSELRVEGVRIKDSPQFHFRFDNCKDVYIDSLH 245
           W   CK   G+ +    D P AI+F  S+ L ++G+++ +SP+FH  F NC+ V I  + 
Sbjct: 174 WAGQCKWVNGREICNDRDRPTAIKFDFSTGLIIQGLKLMNSPEFHLVFGNCEGVKIIGIS 233

Query: 246 ITSPALSPNTDGIHIENTHGVQIFNSIVANGDDCVSIGSGSFNVEIRNITCGPGHGISIG 305
           IT+P  SPNTDGI I  +    +  + +  GDDCV+IG+GS N+ I ++ CGPGHGISIG
Sbjct: 234 ITAPRDSPNTDGIDIFASKNFHLQKNTIGTGDDCVAIGTGSSNIVIEDLICGPGHGISIG 293

Query: 306 SLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGFGTVRGVSFNNINMDNVRNPIIID 365
           SLG  NSRA VS + V  +    + NG+RIKTWQGG G    + + N+ M N  NPI+I+
Sbjct: 294 SLGRENSRAEVSYVHVNGAKFIDTQNGLRIKTWQGGSGMASHIIYENVEMINSENPILIN 353

Query: 366 QFYCLN-KGCLNQTSAVVVSDISYIDIKGTYDIRSPPMHFGCSDSMPCMNLTLANIELFP 425
           QFYC +   C NQ SAV + D++Y +I+GT    +  +   CSDSMPC ++ L++I L  
Sbjct: 354 QFYCTSASACQNQRSAVQIQDVTYKNIRGT-SATAAAIQLKCSDSMPCKDIKLSDISLKL 413

Query: 426 SRGDI---LLDPFCWNAYGNLQTLTIPPISCLLETSSRSFLESEK 467
           + G I   L D    NA G      IP    L  ++ R   +S K
Sbjct: 414 TSGKIASCLND----NANGYFSGHVIPACKNLSPSAKRKESKSHK 443

BLAST of CSPI03G29190 vs. ExPASy Swiss-Prot
Match: Q02096 (Polygalacturonase OS=Persea americana OX=3435 PE=2 SV=1)

HSP 1 Score: 305.1 bits (780), Expect = 1.4e-81
Identity = 162/372 (43.55%), Postives = 219/372 (58.87%), Query Frame = 0

Query: 58  GGPPSSSINSTSIFDVRHFGAIGDGTADDTAAFKMAWDTACQRNDDDNNDDYGVILVPYG 117
           GGP +S     S+ D   FGA GDGT DDT AF+ AW  AC            V++VP  
Sbjct: 75  GGPETSPDTDISVDD---FGARGDGT-DDTKAFEKAWKDACSSG--------SVLIVPEN 134

Query: 118 FSFMIQSTIFTGPCKNGVVFQVDGTLMAPDGPDAWPKGYSAHQWLVFYRVYNMSLQGNGL 177
            +++++   F+GPCK+ +  ++ GT+ A      W  G++  +W+ F  + N++L+G G 
Sbjct: 135 KNYLLKQITFSGPCKSDLRVKIRGTIEASSDQSDW-VGHNRKRWIEFEDISNLTLEGGGT 194

Query: 178 IDGRGQKWWDLPCKPHKGKTMTGPCDS-PIAIRFFMSSELRVEGVRIKDSPQFHFRFDNC 237
            +G G+ WWD  CK  K      PC S P A+ F     L V  + IKDS + H  FD C
Sbjct: 195 SNGNGETWWDSSCKRKKSL----PCKSAPTALTFRSCKNLIVSDLSIKDSQKMHLSFDKC 254

Query: 238 KDVYIDSLHITSPALSPNTDGIHIENTHGVQIFNSIVANGDDCVSIGSGSFNVEIRNITC 297
           +DV   +L +T+P  SPNTDGIHI  T  + + NS++  GDDC+SI SGS  V   NITC
Sbjct: 255 QDVIASNLMVTAPEHSPNTDGIHITGTQRIHVMNSVIGTGDDCISIESGSKMVIATNITC 314

Query: 298 GPGHGISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGFGTVRGVSFNNINMD 357
           GPGHGISIGSLG+ NS A VS + V    +  + NG+RIKTWQGG G+ + + F NI M 
Sbjct: 315 GPGHGISIGSLGDRNSEAHVSGVLVDGGNLFDTTNGLRIKTWQGGSGSAKNIKFQNIVMH 374

Query: 358 NVRNPIIIDQFYCLNKG-CLNQTSAVVVSDISYIDIKGTYDIRSPPMHFGCSDSMPCMNL 417
           NV NPIIIDQ+YC +K  C  Q SAV VS+++Y++I+GT       + F CS S PC   
Sbjct: 375 NVTNPIIIDQYYCDSKDPCPEQESAVKVSNVAYMNIRGT-SASEVAVKFDCSKSSPCQGY 428

Query: 418 TLANIELFPSRG 428
            + NI L  + G
Sbjct: 435 IVGNINLVGNGG 428

BLAST of CSPI03G29190 vs. ExPASy Swiss-Prot
Match: Q8RY29 (Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2)

HSP 1 Score: 304.3 bits (778), Expect = 2.4e-81
Identity = 155/363 (42.70%), Postives = 219/363 (60.33%), Query Frame = 0

Query: 63  SSINSTSIFDVRHFGAIGDGTADDTAAFKMAWDTACQRNDDDNNDDYGVILVPYGFSFMI 122
           +S+ + +   V  FGA GDG  DDT AF  AW  AC  N   N      +LVP G ++++
Sbjct: 60  ASLRTPTTVSVSDFGAKGDGKTDDTQAFVNAWKKACSSNGAVN------LLVPKGNTYLL 119

Query: 123 QSTIFTGPCKNGVVFQVDGTLMAPDGPDAWPKGYSAHQWLVFYRVYNMSLQG--NGLIDG 182
           +S   TGPC + +  Q+ GTL A      +       +W++F  V N+S+ G   G++DG
Sbjct: 120 KSIQLTGPCNSILTVQIFGTLSASQKRSDYK---DISKWIMFDGVNNLSVDGGDTGVVDG 179

Query: 183 RGQKWWDLPCKPHKGKTMTGPC-DSPIAIRFFMSSELRVEGVRIKDSPQFHFRFDNCKDV 242
            G+ WW   CK +K K    PC  +P A+ F+ S  L V+ ++++++ Q     + C +V
Sbjct: 180 NGETWWQNSCKRNKAK----PCTKAPTALTFYNSKSLIVKNLKVRNAQQIQISIEKCSNV 239

Query: 243 YIDSLHITSPALSPNTDGIHIENTHGVQIFNSIVANGDDCVSIGSGSFNVEIRNITCGPG 302
            + ++ +T+PA SPNTDGIHI NT  +++  SI+  GDDC+SI SGS NV+I +ITCGPG
Sbjct: 240 QVSNVVVTAPADSPNTDGIHITNTQNIRVSESIIGTGDDCISIESGSQNVQINDITCGPG 299

Query: 303 HGISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGFGTVRGVSFNNINMDNVR 362
           HGISIGSLG+ NS+A VS +TV  + +  +DNGVRIKT+QGG GT   + F NI MDNV+
Sbjct: 300 HGISIGSLGDDNSKAFVSGVTVDGAKLSGTDNGVRIKTYQGGSGTASNIIFQNIQMDNVK 359

Query: 363 NPIIIDQFYCLNKGCLNQTSAVVVSDISYIDIKGTYDIRSPPMHFGCSDSMPCMNLTLAN 422
           NPIIIDQ YC    C  + SAV V ++ Y DI GT       + F CS + PC  + L  
Sbjct: 360 NPIIIDQDYCDKSKCTTEKSAVQVKNVVYRDISGT-SASENAITFNCSKNYPCQGIVLDR 408

BLAST of CSPI03G29190 vs. ExPASy TrEMBL
Match: A0A0A0LEQ6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G664600 PE=3 SV=1)

HSP 1 Score: 988.4 bits (2554), Expect = 1.0e-284
Identity = 467/469 (99.57%), Postives = 468/469 (99.79%), Query Frame = 0

Query: 1   MTFIIFFFCLLFPVHGRWHNHAKKHKQHSHHHDHFTISQPPSPSPSSEPAGSPPEDNGGP 60
           MTFIIFFFCLLFPVHGRWHNHAKKHKQHSHHHDHFTISQPPSPSPSSEPAGSPPEDNGGP
Sbjct: 1   MTFIIFFFCLLFPVHGRWHNHAKKHKQHSHHHDHFTISQPPSPSPSSEPAGSPPEDNGGP 60

Query: 61  PSSSINSTSIFDVRHFGAIGDGTADDTAAFKMAWDTACQRNDDDNNDDYGVILVPYGFSF 120
            SSSINSTSIFDVRHFGAIGDGTADDTAAFKMAWDTACQRNDDDNNDDYGVILVPYGFSF
Sbjct: 61  SSSSINSTSIFDVRHFGAIGDGTADDTAAFKMAWDTACQRNDDDNNDDYGVILVPYGFSF 120

Query: 121 MIQSTIFTGPCKNGVVFQVDGTLMAPDGPDAWPKGYSAHQWLVFYRVYNMSLQGNGLIDG 180
           MIQSTIFTGPCKNGVVFQVDGTLMAPDGPDAWPKGYSAHQWLVFYRVYNMSLQGNGLIDG
Sbjct: 121 MIQSTIFTGPCKNGVVFQVDGTLMAPDGPDAWPKGYSAHQWLVFYRVYNMSLQGNGLIDG 180

Query: 181 RGQKWWDLPCKPHKGKTMTGPCDSPIAIRFFMSSELRVEGVRIKDSPQFHFRFDNCKDVY 240
           RGQKWWDLPCKPHKGKTMTGPCDSPIAIRFFMSSELRVEGVRIKDSPQFHFRFDNCKDVY
Sbjct: 181 RGQKWWDLPCKPHKGKTMTGPCDSPIAIRFFMSSELRVEGVRIKDSPQFHFRFDNCKDVY 240

Query: 241 IDSLHITSPALSPNTDGIHIENTHGVQIFNSIVANGDDCVSIGSGSFNVEIRNITCGPGH 300
           IDSLHITSPALSPNTDGIHIENT+GVQIFNSIVANGDDCVSIGSGSFNVEIRNITCGPGH
Sbjct: 241 IDSLHITSPALSPNTDGIHIENTNGVQIFNSIVANGDDCVSIGSGSFNVEIRNITCGPGH 300

Query: 301 GISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGFGTVRGVSFNNINMDNVRN 360
           GISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGFGTVRGVSFNNINMDNVRN
Sbjct: 301 GISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGFGTVRGVSFNNINMDNVRN 360

Query: 361 PIIIDQFYCLNKGCLNQTSAVVVSDISYIDIKGTYDIRSPPMHFGCSDSMPCMNLTLANI 420
           PIIIDQFYCLNKGCLNQTSAVVVSDISYIDIKGTYDIRSPPMHFGCSDSMPCMNLTLANI
Sbjct: 361 PIIIDQFYCLNKGCLNQTSAVVVSDISYIDIKGTYDIRSPPMHFGCSDSMPCMNLTLANI 420

Query: 421 ELFPSRGDILLDPFCWNAYGNLQTLTIPPISCLLETSSRSFLESEKDYC 470
           ELFPSRGDILLDPFCWNAYGNLQTLTIPPISCLLETSSRSFLESEKDYC
Sbjct: 421 ELFPSRGDILLDPFCWNAYGNLQTLTIPPISCLLETSSRSFLESEKDYC 469

BLAST of CSPI03G29190 vs. ExPASy TrEMBL
Match: A0A5D3DA93 (Polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold237G00750 PE=3 SV=1)

HSP 1 Score: 932.6 bits (2409), Expect = 6.6e-268
Identity = 447/481 (92.93%), Postives = 461/481 (95.84%), Query Frame = 0

Query: 1   MTFIIFFFCLLFPVHGRWHNHAKKHKQHSHHHDHFTISQP------------PSPSPSSE 60
           +TFIIFFFCLLFPV+GRWHNHAKKHKQHSHHH HFTISQP            PSPSPSSE
Sbjct: 15  ITFIIFFFCLLFPVNGRWHNHAKKHKQHSHHHYHFTISQPPSPFPSPSPSPSPSPSPSSE 74

Query: 61  PAGSPPEDNGGPPSSSINSTSIFDVRHFGAIGDGTADDTAAFKMAWDTACQRNDDDNNDD 120
           P  SPPED+GG P+SSINST IFDVRHFGAIGDGTADDTAAFKMAWDTACQRNDDD+N +
Sbjct: 75  PQ-SPPEDDGG-PASSINSTIIFDVRHFGAIGDGTADDTAAFKMAWDTACQRNDDDDN-N 134

Query: 121 YGVILVPYGFSFMIQSTIFTGPCKNGVVFQVDGTLMAPDGPDAWPKGYSAHQWLVFYRVY 180
           YGVILVPYGFSFMIQSTIFTGPC+NG+VFQVDGTLMAPDGPDAWPKGYSAHQWLVFYRVY
Sbjct: 135 YGVILVPYGFSFMIQSTIFTGPCRNGIVFQVDGTLMAPDGPDAWPKGYSAHQWLVFYRVY 194

Query: 181 NMSLQGNGLIDGRGQKWWDLPCKPHKGKTMTGPCDSPIAIRFFMSSELRVEGVRIKDSPQ 240
           NMSLQGNGLIDGRGQKWWDLPCKPHKGKTMTGPCDSPIAIRFFMSS+LRVEGVRIKDSPQ
Sbjct: 195 NMSLQGNGLIDGRGQKWWDLPCKPHKGKTMTGPCDSPIAIRFFMSSKLRVEGVRIKDSPQ 254

Query: 241 FHFRFDNCKDVYIDSLHITSPALSPNTDGIHIENTHGVQIFNSIVANGDDCVSIGSGSFN 300
           FHFRFDNCKDVYIDSLHITSPALSPNTDGIHIENT+GVQIFNSIVANGDDC+SIGSGS+N
Sbjct: 255 FHFRFDNCKDVYIDSLHITSPALSPNTDGIHIENTNGVQIFNSIVANGDDCISIGSGSYN 314

Query: 301 VEIRNITCGPGHGISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGFGTVRGV 360
           VEI NITCGPGHGISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGG+G VRGV
Sbjct: 315 VEITNITCGPGHGISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGYGAVRGV 374

Query: 361 SFNNINMDNVRNPIIIDQFYCLNKGCLNQTSAVVVSDISYIDIKGTYDIRSPPMHFGCSD 420
           SFNNINMDNVRNPIIIDQFYCLNKGCLNQTSAVVVSDISYIDIKGTYDIRSPPMHFGCSD
Sbjct: 375 SFNNINMDNVRNPIIIDQFYCLNKGCLNQTSAVVVSDISYIDIKGTYDIRSPPMHFGCSD 434

Query: 421 SMPCMNLTLANIELFPSRGDILLDPFCWNAYGNLQTLTIPPISCLLETSSRSFLESEKDY 470
           SMPCMNLTLANIELFPS+GDILLDPFCWNAYGNLQTLTIPPISCLLETSSRSFLESEKDY
Sbjct: 435 SMPCMNLTLANIELFPSKGDILLDPFCWNAYGNLQTLTIPPISCLLETSSRSFLESEKDY 492

BLAST of CSPI03G29190 vs. ExPASy TrEMBL
Match: A0A5A7T924 (Polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold36G003270 PE=3 SV=1)

HSP 1 Score: 899.8 bits (2324), Expect = 4.8e-258
Identity = 436/481 (90.64%), Postives = 453/481 (94.18%), Query Frame = 0

Query: 1   MTFIIFFFCLLFPVHGRWHNHAKKHKQHSHHHDHFTISQP------------PSPSPSSE 60
           +TFIIFFFCLLFPV+GRWHNHAKKHKQHSHHH HFTISQP            PSPSPSSE
Sbjct: 15  ITFIIFFFCLLFPVNGRWHNHAKKHKQHSHHHYHFTISQPPSPFPSPSPSPSPSPSPSSE 74

Query: 61  PAGSPPEDNGGPPSSSINSTSIFDVRHFGAIGDGTADDTAAFKMAWDTACQRNDDDNNDD 120
           P  SPPED+GG P+SSINST IFDVRHFGAIGDGTADDTAAFKMAWDTACQRNDDD+N +
Sbjct: 75  PQ-SPPEDDGG-PASSINSTIIFDVRHFGAIGDGTADDTAAFKMAWDTACQRNDDDDN-N 134

Query: 121 YGVILVPYGFSFMIQSTIFTGPCKNGVVFQVDGTLMAPDGPDAWPKGYSAHQWLVFYRVY 180
           YGVILVPYGFSFMIQSTIFTGPC+NG+VFQVDGTLMAPDGPDAWPKGYSAHQWLVFYRVY
Sbjct: 135 YGVILVPYGFSFMIQSTIFTGPCRNGIVFQVDGTLMAPDGPDAWPKGYSAHQWLVFYRVY 194

Query: 181 NMSLQGNGLIDGRGQKWWDLPCKPHKGKTMTGPCDSPIAIRFFMSSELRVEGVRIKDSPQ 240
           NMSLQGNGLIDGRGQKWWDLPCKPHK K+++       AIRFFMSS+LRVEGVRIKDSPQ
Sbjct: 195 NMSLQGNGLIDGRGQKWWDLPCKPHKSKSVSFFWQ---AIRFFMSSKLRVEGVRIKDSPQ 254

Query: 241 FHFRFDNCKDVYIDSLHITSPALSPNTDGIHIENTHGVQIFNSIVANGDDCVSIGSGSFN 300
           FHFRFDNCKDVYIDSLHITSPALSPNTDGIHIENT+GVQIFNSIVANGDDC+SIGSGS+N
Sbjct: 255 FHFRFDNCKDVYIDSLHITSPALSPNTDGIHIENTNGVQIFNSIVANGDDCISIGSGSYN 314

Query: 301 VEIRNITCGPGHGISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGFGTVRGV 360
           VEI NITCGPGHGISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGG+G VRGV
Sbjct: 315 VEITNITCGPGHGISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGYGAVRGV 374

Query: 361 SFNNINMDNVRNPIIIDQFYCLNKGCLNQTSAVVVSDISYIDIKGTYDIRSPPMHFGCSD 420
           SFNNINMDNVRNPIIIDQFYCLNKGCLNQTSAVVVSDISYIDIKGTYDIRSPPMHFGCSD
Sbjct: 375 SFNNINMDNVRNPIIIDQFYCLNKGCLNQTSAVVVSDISYIDIKGTYDIRSPPMHFGCSD 434

Query: 421 SMPCMNLTLANIELFPSRGDILLDPFCWNAYGNLQTLTIPPISCLLETSSRSFLESEKDY 470
           SMPCMNLTLANIELFPS+GDILLDPFCWNAYGNLQTLTIPPISCLLETSSRSFLESEKDY
Sbjct: 435 SMPCMNLTLANIELFPSKGDILLDPFCWNAYGNLQTLTIPPISCLLETSSRSFLESEKDY 489

BLAST of CSPI03G29190 vs. ExPASy TrEMBL
Match: A0A1S4DWP4 (polygalacturonase At1g48100 OS=Cucumis melo OX=3656 GN=LOC103490076 PE=3 SV=1)

HSP 1 Score: 858.6 bits (2217), Expect = 1.2e-245
Identity = 408/427 (95.55%), Postives = 420/427 (98.36%), Query Frame = 0

Query: 43  PSPSSEPAGSPPEDNGGPPSSSINSTSIFDVRHFGAIGDGTADDTAAFKMAWDTACQRND 102
           PSPSSEP  SPPED+GG P+SSINST IFDVRHFGAIGDGTADDTAAFKMAWDTACQRND
Sbjct: 35  PSPSSEPQ-SPPEDDGG-PASSINSTIIFDVRHFGAIGDGTADDTAAFKMAWDTACQRND 94

Query: 103 DDNNDDYGVILVPYGFSFMIQSTIFTGPCKNGVVFQVDGTLMAPDGPDAWPKGYSAHQWL 162
           DD+N +YGVILVPYGFSFMIQSTIFTGPC+NG+VFQVDGTLMAPDGPDAWPKGYSAHQWL
Sbjct: 95  DDDN-NYGVILVPYGFSFMIQSTIFTGPCRNGIVFQVDGTLMAPDGPDAWPKGYSAHQWL 154

Query: 163 VFYRVYNMSLQGNGLIDGRGQKWWDLPCKPHKGKTMTGPCDSPIAIRFFMSSELRVEGVR 222
           VFYRVYNMSLQGNGLIDGRGQKWWDLPCKPHKGKTMTGPCDSPIAIRFFMSS+LRVEGVR
Sbjct: 155 VFYRVYNMSLQGNGLIDGRGQKWWDLPCKPHKGKTMTGPCDSPIAIRFFMSSKLRVEGVR 214

Query: 223 IKDSPQFHFRFDNCKDVYIDSLHITSPALSPNTDGIHIENTHGVQIFNSIVANGDDCVSI 282
           IKDSPQFHFRFDNCKDVYIDSLHITSPALSPNTDGIHIENT+GVQIFNSIVANGDDC+SI
Sbjct: 215 IKDSPQFHFRFDNCKDVYIDSLHITSPALSPNTDGIHIENTNGVQIFNSIVANGDDCISI 274

Query: 283 GSGSFNVEIRNITCGPGHGISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGF 342
           GSGS+NVEI NITCGPGHGISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGG+
Sbjct: 275 GSGSYNVEITNITCGPGHGISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGY 334

Query: 343 GTVRGVSFNNINMDNVRNPIIIDQFYCLNKGCLNQTSAVVVSDISYIDIKGTYDIRSPPM 402
           G VRGVSFNNINMDNVRNPIIIDQFYCLNKGCLNQTSAVVVSDISYIDIKGTYDIRSPPM
Sbjct: 335 GAVRGVSFNNINMDNVRNPIIIDQFYCLNKGCLNQTSAVVVSDISYIDIKGTYDIRSPPM 394

Query: 403 HFGCSDSMPCMNLTLANIELFPSRGDILLDPFCWNAYGNLQTLTIPPISCLLETSSRSFL 462
           HFGCSDSMPCMNLTLANIELFPS+GDILLDPFCWNAYGNLQTLTIPPISCLLETSSRSFL
Sbjct: 395 HFGCSDSMPCMNLTLANIELFPSKGDILLDPFCWNAYGNLQTLTIPPISCLLETSSRSFL 454

Query: 463 ESEKDYC 470
           ESEKDYC
Sbjct: 455 ESEKDYC 458

BLAST of CSPI03G29190 vs. ExPASy TrEMBL
Match: A0A6J1FAK7 (polygalacturonase At1g48100-like OS=Cucurbita moschata OX=3662 GN=LOC111442323 PE=3 SV=1)

HSP 1 Score: 834.7 bits (2155), Expect = 1.9e-238
Identity = 398/479 (83.09%), Postives = 427/479 (89.14%), Query Frame = 0

Query: 1   MTFIIFFFCLLFPVHGRWHNHAKKHKQHSHHHDHFTISQPPSPSPSSEP-------AGSP 60
           +T IIFF    FPVHGRWHNHAK HK HSHHH HFTISQPPSPSPS  P         +P
Sbjct: 12  ITLIIFF----FPVHGRWHNHAKNHK-HSHHHPHFTISQPPSPSPSPSPPLSPSPEPANP 71

Query: 61  PEDNGGPPSSSINSTSIFDVRHFGAIGDGTADDTAAFKMAWDTACQRNDDDNNDDYGVIL 120
           P+DN G   SS N+T  FDVRH+GAIGDGT DDT AFKMAWDTACQ     +NDDY VIL
Sbjct: 72  PDDNDG--GSSFNATGFFDVRHYGAIGDGTTDDTEAFKMAWDTACQ----SDNDDYAVIL 131

Query: 121 VPYGFSFMIQSTIFTGPCKNGVVFQVDGTLMAPDGPDAWPKGYSAHQWLVFYRVYNMSLQ 180
           VPYGFSFMIQSTIFTGPCK+G+VFQVDGT +APDGPDAWPK YSAHQWLVFYRV NMSL+
Sbjct: 132 VPYGFSFMIQSTIFTGPCKHGIVFQVDGTFIAPDGPDAWPKSYSAHQWLVFYRVNNMSLR 191

Query: 181 GNGLIDGRGQKWWDLPCKPH---KGKTMTGPCDSPIAIRFFMSSELRVEGVRIKDSPQFH 240
           GNG+IDGRGQKWWDLPCKPH   KG+T  GPCDSPIAIRFFMSS L VEG+RIKDSPQFH
Sbjct: 192 GNGVIDGRGQKWWDLPCKPHRGGKGRTKAGPCDSPIAIRFFMSSNLTVEGLRIKDSPQFH 251

Query: 241 FRFDNCKDVYIDSLHITSPALSPNTDGIHIENTHGVQIFNSIVANGDDCVSIGSGSFNVE 300
           FRFDNC+DVYIDSL IT+PA SPNTDGIHIENT+GVQI NS+VANGDDC+SIGSGS+NV+
Sbjct: 252 FRFDNCRDVYIDSLTITAPAWSPNTDGIHIENTNGVQILNSVVANGDDCISIGSGSYNVD 311

Query: 301 IRNITCGPGHGISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGFGTVRGVSF 360
           IRNITCGPGHGISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGFG VRGVSF
Sbjct: 312 IRNITCGPGHGISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGFGAVRGVSF 371

Query: 361 NNINMDNVRNPIIIDQFYCLNKGCLNQTSAVVVSDISYIDIKGTYDIRSPPMHFGCSDSM 420
           NNI+MDNVRNPIIIDQFYCLNKGCLNQTSAVVVSDISYIDIKG+YDIRSPPMHFGCSDSM
Sbjct: 372 NNIHMDNVRNPIIIDQFYCLNKGCLNQTSAVVVSDISYIDIKGSYDIRSPPMHFGCSDSM 431

Query: 421 PCMNLTLANIELFPSRGDILLDPFCWNAYGNLQTLTIPPISCLLETSSRSFLESEKDYC 470
           PC+NLTLANIELFPS+GDI LDPFCWNAYG+LQTLTIPP+ CLLE SSRS LE+++DYC
Sbjct: 432 PCVNLTLANIELFPSKGDIFLDPFCWNAYGDLQTLTIPPLGCLLEASSRSILETQRDYC 479

BLAST of CSPI03G29190 vs. NCBI nr
Match: XP_031737994.1 (polygalacturonase At1g48100 [Cucumis sativus] >KGN58717.2 hypothetical protein Csa_000831 [Cucumis sativus])

HSP 1 Score: 988.4 bits (2554), Expect = 2.1e-284
Identity = 467/469 (99.57%), Postives = 468/469 (99.79%), Query Frame = 0

Query: 1   MTFIIFFFCLLFPVHGRWHNHAKKHKQHSHHHDHFTISQPPSPSPSSEPAGSPPEDNGGP 60
           MTFIIFFFCLLFPVHGRWHNHAKKHKQHSHHHDHFTISQPPSPSPSSEPAGSPPEDNGGP
Sbjct: 15  MTFIIFFFCLLFPVHGRWHNHAKKHKQHSHHHDHFTISQPPSPSPSSEPAGSPPEDNGGP 74

Query: 61  PSSSINSTSIFDVRHFGAIGDGTADDTAAFKMAWDTACQRNDDDNNDDYGVILVPYGFSF 120
            SSSINSTSIFDVRHFGAIGDGTADDTAAFKMAWDTACQRNDDDNNDDYGVILVPYGFSF
Sbjct: 75  SSSSINSTSIFDVRHFGAIGDGTADDTAAFKMAWDTACQRNDDDNNDDYGVILVPYGFSF 134

Query: 121 MIQSTIFTGPCKNGVVFQVDGTLMAPDGPDAWPKGYSAHQWLVFYRVYNMSLQGNGLIDG 180
           MIQSTIFTGPCKNGVVFQVDGTLMAPDGPDAWPKGYSAHQWLVFYRVYNMSLQGNGLIDG
Sbjct: 135 MIQSTIFTGPCKNGVVFQVDGTLMAPDGPDAWPKGYSAHQWLVFYRVYNMSLQGNGLIDG 194

Query: 181 RGQKWWDLPCKPHKGKTMTGPCDSPIAIRFFMSSELRVEGVRIKDSPQFHFRFDNCKDVY 240
           RGQKWWDLPCKPHKGKTMTGPCDSPIAIRFFMSSELRVEGVRIKDSPQFHFRFDNCKDVY
Sbjct: 195 RGQKWWDLPCKPHKGKTMTGPCDSPIAIRFFMSSELRVEGVRIKDSPQFHFRFDNCKDVY 254

Query: 241 IDSLHITSPALSPNTDGIHIENTHGVQIFNSIVANGDDCVSIGSGSFNVEIRNITCGPGH 300
           IDSLHITSPALSPNTDGIHIENT+GVQIFNSIVANGDDCVSIGSGSFNVEIRNITCGPGH
Sbjct: 255 IDSLHITSPALSPNTDGIHIENTNGVQIFNSIVANGDDCVSIGSGSFNVEIRNITCGPGH 314

Query: 301 GISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGFGTVRGVSFNNINMDNVRN 360
           GISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGFGTVRGVSFNNINMDNVRN
Sbjct: 315 GISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGFGTVRGVSFNNINMDNVRN 374

Query: 361 PIIIDQFYCLNKGCLNQTSAVVVSDISYIDIKGTYDIRSPPMHFGCSDSMPCMNLTLANI 420
           PIIIDQFYCLNKGCLNQTSAVVVSDISYIDIKGTYDIRSPPMHFGCSDSMPCMNLTLANI
Sbjct: 375 PIIIDQFYCLNKGCLNQTSAVVVSDISYIDIKGTYDIRSPPMHFGCSDSMPCMNLTLANI 434

Query: 421 ELFPSRGDILLDPFCWNAYGNLQTLTIPPISCLLETSSRSFLESEKDYC 470
           ELFPSRGDILLDPFCWNAYGNLQTLTIPPISCLLETSSRSFLESEKDYC
Sbjct: 435 ELFPSRGDILLDPFCWNAYGNLQTLTIPPISCLLETSSRSFLESEKDYC 483

BLAST of CSPI03G29190 vs. NCBI nr
Match: TYK20501.1 (polygalacturonase [Cucumis melo var. makuwa])

HSP 1 Score: 932.6 bits (2409), Expect = 1.4e-267
Identity = 447/481 (92.93%), Postives = 461/481 (95.84%), Query Frame = 0

Query: 1   MTFIIFFFCLLFPVHGRWHNHAKKHKQHSHHHDHFTISQP------------PSPSPSSE 60
           +TFIIFFFCLLFPV+GRWHNHAKKHKQHSHHH HFTISQP            PSPSPSSE
Sbjct: 15  ITFIIFFFCLLFPVNGRWHNHAKKHKQHSHHHYHFTISQPPSPFPSPSPSPSPSPSPSSE 74

Query: 61  PAGSPPEDNGGPPSSSINSTSIFDVRHFGAIGDGTADDTAAFKMAWDTACQRNDDDNNDD 120
           P  SPPED+GG P+SSINST IFDVRHFGAIGDGTADDTAAFKMAWDTACQRNDDD+N +
Sbjct: 75  PQ-SPPEDDGG-PASSINSTIIFDVRHFGAIGDGTADDTAAFKMAWDTACQRNDDDDN-N 134

Query: 121 YGVILVPYGFSFMIQSTIFTGPCKNGVVFQVDGTLMAPDGPDAWPKGYSAHQWLVFYRVY 180
           YGVILVPYGFSFMIQSTIFTGPC+NG+VFQVDGTLMAPDGPDAWPKGYSAHQWLVFYRVY
Sbjct: 135 YGVILVPYGFSFMIQSTIFTGPCRNGIVFQVDGTLMAPDGPDAWPKGYSAHQWLVFYRVY 194

Query: 181 NMSLQGNGLIDGRGQKWWDLPCKPHKGKTMTGPCDSPIAIRFFMSSELRVEGVRIKDSPQ 240
           NMSLQGNGLIDGRGQKWWDLPCKPHKGKTMTGPCDSPIAIRFFMSS+LRVEGVRIKDSPQ
Sbjct: 195 NMSLQGNGLIDGRGQKWWDLPCKPHKGKTMTGPCDSPIAIRFFMSSKLRVEGVRIKDSPQ 254

Query: 241 FHFRFDNCKDVYIDSLHITSPALSPNTDGIHIENTHGVQIFNSIVANGDDCVSIGSGSFN 300
           FHFRFDNCKDVYIDSLHITSPALSPNTDGIHIENT+GVQIFNSIVANGDDC+SIGSGS+N
Sbjct: 255 FHFRFDNCKDVYIDSLHITSPALSPNTDGIHIENTNGVQIFNSIVANGDDCISIGSGSYN 314

Query: 301 VEIRNITCGPGHGISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGFGTVRGV 360
           VEI NITCGPGHGISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGG+G VRGV
Sbjct: 315 VEITNITCGPGHGISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGYGAVRGV 374

Query: 361 SFNNINMDNVRNPIIIDQFYCLNKGCLNQTSAVVVSDISYIDIKGTYDIRSPPMHFGCSD 420
           SFNNINMDNVRNPIIIDQFYCLNKGCLNQTSAVVVSDISYIDIKGTYDIRSPPMHFGCSD
Sbjct: 375 SFNNINMDNVRNPIIIDQFYCLNKGCLNQTSAVVVSDISYIDIKGTYDIRSPPMHFGCSD 434

Query: 421 SMPCMNLTLANIELFPSRGDILLDPFCWNAYGNLQTLTIPPISCLLETSSRSFLESEKDY 470
           SMPCMNLTLANIELFPS+GDILLDPFCWNAYGNLQTLTIPPISCLLETSSRSFLESEKDY
Sbjct: 435 SMPCMNLTLANIELFPSKGDILLDPFCWNAYGNLQTLTIPPISCLLETSSRSFLESEKDY 492

BLAST of CSPI03G29190 vs. NCBI nr
Match: KAA0038107.1 (polygalacturonase [Cucumis melo var. makuwa])

HSP 1 Score: 899.8 bits (2324), Expect = 9.8e-258
Identity = 436/481 (90.64%), Postives = 453/481 (94.18%), Query Frame = 0

Query: 1   MTFIIFFFCLLFPVHGRWHNHAKKHKQHSHHHDHFTISQP------------PSPSPSSE 60
           +TFIIFFFCLLFPV+GRWHNHAKKHKQHSHHH HFTISQP            PSPSPSSE
Sbjct: 15  ITFIIFFFCLLFPVNGRWHNHAKKHKQHSHHHYHFTISQPPSPFPSPSPSPSPSPSPSSE 74

Query: 61  PAGSPPEDNGGPPSSSINSTSIFDVRHFGAIGDGTADDTAAFKMAWDTACQRNDDDNNDD 120
           P  SPPED+GG P+SSINST IFDVRHFGAIGDGTADDTAAFKMAWDTACQRNDDD+N +
Sbjct: 75  PQ-SPPEDDGG-PASSINSTIIFDVRHFGAIGDGTADDTAAFKMAWDTACQRNDDDDN-N 134

Query: 121 YGVILVPYGFSFMIQSTIFTGPCKNGVVFQVDGTLMAPDGPDAWPKGYSAHQWLVFYRVY 180
           YGVILVPYGFSFMIQSTIFTGPC+NG+VFQVDGTLMAPDGPDAWPKGYSAHQWLVFYRVY
Sbjct: 135 YGVILVPYGFSFMIQSTIFTGPCRNGIVFQVDGTLMAPDGPDAWPKGYSAHQWLVFYRVY 194

Query: 181 NMSLQGNGLIDGRGQKWWDLPCKPHKGKTMTGPCDSPIAIRFFMSSELRVEGVRIKDSPQ 240
           NMSLQGNGLIDGRGQKWWDLPCKPHK K+++       AIRFFMSS+LRVEGVRIKDSPQ
Sbjct: 195 NMSLQGNGLIDGRGQKWWDLPCKPHKSKSVSFFWQ---AIRFFMSSKLRVEGVRIKDSPQ 254

Query: 241 FHFRFDNCKDVYIDSLHITSPALSPNTDGIHIENTHGVQIFNSIVANGDDCVSIGSGSFN 300
           FHFRFDNCKDVYIDSLHITSPALSPNTDGIHIENT+GVQIFNSIVANGDDC+SIGSGS+N
Sbjct: 255 FHFRFDNCKDVYIDSLHITSPALSPNTDGIHIENTNGVQIFNSIVANGDDCISIGSGSYN 314

Query: 301 VEIRNITCGPGHGISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGFGTVRGV 360
           VEI NITCGPGHGISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGG+G VRGV
Sbjct: 315 VEITNITCGPGHGISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGYGAVRGV 374

Query: 361 SFNNINMDNVRNPIIIDQFYCLNKGCLNQTSAVVVSDISYIDIKGTYDIRSPPMHFGCSD 420
           SFNNINMDNVRNPIIIDQFYCLNKGCLNQTSAVVVSDISYIDIKGTYDIRSPPMHFGCSD
Sbjct: 375 SFNNINMDNVRNPIIIDQFYCLNKGCLNQTSAVVVSDISYIDIKGTYDIRSPPMHFGCSD 434

Query: 421 SMPCMNLTLANIELFPSRGDILLDPFCWNAYGNLQTLTIPPISCLLETSSRSFLESEKDY 470
           SMPCMNLTLANIELFPS+GDILLDPFCWNAYGNLQTLTIPPISCLLETSSRSFLESEKDY
Sbjct: 435 SMPCMNLTLANIELFPSKGDILLDPFCWNAYGNLQTLTIPPISCLLETSSRSFLESEKDY 489

BLAST of CSPI03G29190 vs. NCBI nr
Match: XP_038905655.1 (polygalacturonase At1g48100-like [Benincasa hispida])

HSP 1 Score: 885.6 bits (2287), Expect = 1.9e-253
Identity = 418/472 (88.56%), Postives = 445/472 (94.28%), Query Frame = 0

Query: 1   MTFIIFFFCLLFPVHGRWHNHAKKHKQHSHHHDHFTISQPPSPSPSSEPAGSPPEDNGGP 60
           +TFIIFFFCLLFPVHGRWHNHAKKHKQHSHHH+HFTISQPPSP P+S P     +DNGG 
Sbjct: 13  ITFIIFFFCLLFPVHGRWHNHAKKHKQHSHHHNHFTISQPPSPPPTSPP---DDDDNGG- 72

Query: 61  PSSSINSTSIFDVRHFGAIGDGTADDTAAFKMAWDTACQRNDDDNNDDYGVILVPYGFSF 120
            SSSINST IFDVR+FGAIGDGTADDT AFKMAWDTACQR+DDD  D+Y VIL+PYGFSF
Sbjct: 73  -SSSINSTGIFDVRNFGAIGDGTADDTMAFKMAWDTACQRDDDD--DNYAVILIPYGFSF 132

Query: 121 MIQSTIFTGPCKNGVVFQVDGTLMAPDGPDAWPKGYSAHQWLVFYRVYNMSLQGNGLIDG 180
           MIQSTIFTGPCKNGV FQV GTLMAPDGPDAWPK YSAHQWLVFYRVYNMSL+GNGLIDG
Sbjct: 133 MIQSTIFTGPCKNGVEFQVGGTLMAPDGPDAWPKSYSAHQWLVFYRVYNMSLRGNGLIDG 192

Query: 181 RGQKWWDLPCKPH---KGKTMTGPCDSPIAIRFFMSSELRVEGVRIKDSPQFHFRFDNCK 240
           RGQKWWDLPCKPH   KG+TM+ PCDSPIAIRFFMSS L VEG+RIKDSPQFHFRFDNCK
Sbjct: 193 RGQKWWDLPCKPHKGVKGRTMSEPCDSPIAIRFFMSSNLTVEGLRIKDSPQFHFRFDNCK 252

Query: 241 DVYIDSLHITSPALSPNTDGIHIENTHGVQIFNSIVANGDDCVSIGSGSFNVEIRNITCG 300
           DV ID +HIT+PALSPNTDGIHIENT+GVQIFNS VANGDDC+SIGSGS+NV+IRNITCG
Sbjct: 253 DVIIDGIHITAPALSPNTDGIHIENTNGVQIFNSEVANGDDCISIGSGSYNVDIRNITCG 312

Query: 301 PGHGISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGFGTVRGVSFNNINMDN 360
           PGHGISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGG+G VRGVSFNNINMDN
Sbjct: 313 PGHGISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGYGAVRGVSFNNINMDN 372

Query: 361 VRNPIIIDQFYCLNKGCLNQTSAVVVSDISYIDIKGTYDIRSPPMHFGCSDSMPCMNLTL 420
           VRNPIIIDQFYCLNKGCLNQTSAVVVSDI+Y+DIKGTYDIRSPPMHFGCSDSMPCMNLTL
Sbjct: 373 VRNPIIIDQFYCLNKGCLNQTSAVVVSDITYVDIKGTYDIRSPPMHFGCSDSMPCMNLTL 432

Query: 421 ANIELFPSRGDILLDPFCWNAYGNLQTLTIPPISCLLETSSRSFLESEKDYC 470
           ANIELFPS+G++LLDPFCWNAYGNLQTLTIPP++CLLE+SSRSFLESEKDYC
Sbjct: 433 ANIELFPSKGEVLLDPFCWNAYGNLQTLTIPPVACLLESSSRSFLESEKDYC 477

BLAST of CSPI03G29190 vs. NCBI nr
Match: XP_016900401.1 (PREDICTED: polygalacturonase At1g48100, partial [Cucumis melo])

HSP 1 Score: 858.6 bits (2217), Expect = 2.5e-245
Identity = 408/427 (95.55%), Postives = 420/427 (98.36%), Query Frame = 0

Query: 43  PSPSSEPAGSPPEDNGGPPSSSINSTSIFDVRHFGAIGDGTADDTAAFKMAWDTACQRND 102
           PSPSSEP  SPPED+GG P+SSINST IFDVRHFGAIGDGTADDTAAFKMAWDTACQRND
Sbjct: 35  PSPSSEPQ-SPPEDDGG-PASSINSTIIFDVRHFGAIGDGTADDTAAFKMAWDTACQRND 94

Query: 103 DDNNDDYGVILVPYGFSFMIQSTIFTGPCKNGVVFQVDGTLMAPDGPDAWPKGYSAHQWL 162
           DD+N +YGVILVPYGFSFMIQSTIFTGPC+NG+VFQVDGTLMAPDGPDAWPKGYSAHQWL
Sbjct: 95  DDDN-NYGVILVPYGFSFMIQSTIFTGPCRNGIVFQVDGTLMAPDGPDAWPKGYSAHQWL 154

Query: 163 VFYRVYNMSLQGNGLIDGRGQKWWDLPCKPHKGKTMTGPCDSPIAIRFFMSSELRVEGVR 222
           VFYRVYNMSLQGNGLIDGRGQKWWDLPCKPHKGKTMTGPCDSPIAIRFFMSS+LRVEGVR
Sbjct: 155 VFYRVYNMSLQGNGLIDGRGQKWWDLPCKPHKGKTMTGPCDSPIAIRFFMSSKLRVEGVR 214

Query: 223 IKDSPQFHFRFDNCKDVYIDSLHITSPALSPNTDGIHIENTHGVQIFNSIVANGDDCVSI 282
           IKDSPQFHFRFDNCKDVYIDSLHITSPALSPNTDGIHIENT+GVQIFNSIVANGDDC+SI
Sbjct: 215 IKDSPQFHFRFDNCKDVYIDSLHITSPALSPNTDGIHIENTNGVQIFNSIVANGDDCISI 274

Query: 283 GSGSFNVEIRNITCGPGHGISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGF 342
           GSGS+NVEI NITCGPGHGISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGG+
Sbjct: 275 GSGSYNVEITNITCGPGHGISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGY 334

Query: 343 GTVRGVSFNNINMDNVRNPIIIDQFYCLNKGCLNQTSAVVVSDISYIDIKGTYDIRSPPM 402
           G VRGVSFNNINMDNVRNPIIIDQFYCLNKGCLNQTSAVVVSDISYIDIKGTYDIRSPPM
Sbjct: 335 GAVRGVSFNNINMDNVRNPIIIDQFYCLNKGCLNQTSAVVVSDISYIDIKGTYDIRSPPM 394

Query: 403 HFGCSDSMPCMNLTLANIELFPSRGDILLDPFCWNAYGNLQTLTIPPISCLLETSSRSFL 462
           HFGCSDSMPCMNLTLANIELFPS+GDILLDPFCWNAYGNLQTLTIPPISCLLETSSRSFL
Sbjct: 395 HFGCSDSMPCMNLTLANIELFPSKGDILLDPFCWNAYGNLQTLTIPPISCLLETSSRSFL 454

Query: 463 ESEKDYC 470
           ESEKDYC
Sbjct: 455 ESEKDYC 458

BLAST of CSPI03G29190 vs. TAIR 10
Match: AT1G02460.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 630.6 bits (1625), Expect = 1.0e-180
Identity = 302/481 (62.79%), Postives = 375/481 (77.96%), Query Frame = 0

Query: 3   FIIFFFCLLFPVHGRWHNHAKKHKQHSHHH-------DHFTISQPPS--PSPSSEPAGSP 62
           F+     L+     R H+H +KHK ++H+H          +ISQPP+  P P   PA S 
Sbjct: 14  FMTLILTLMSLSEARNHHHKEKHKHNNHNHHSSKPKPPSSSISQPPTPPPGPPDSPAPSL 73

Query: 63  PEDNGGPPSSSINSTSIFDVRHFGAIGDGTADDTAAFKMAWDTACQRNDDDNNDDYGVIL 122
           P      P+   N   I++VR +GA+GDG  DDT AFK AWD++C  N+++N D   V+L
Sbjct: 74  PPSPSDDPADDNN--GIYNVRKYGAVGDGETDDTEAFKTAWDSSC--NNENNTD--SVLL 133

Query: 123 VPYGFSFMIQSTIFTGPCKNGVVFQVDGTLMAPDGPDAWPKGYSAHQWLVFYRVYNMSLQ 182
           VPYG++FMIQSTIFTGPC++   FQVDGT++ PDGP++WP   S  QWLVFYRV  M+L+
Sbjct: 134 VPYGYTFMIQSTIFTGPCRSYQFFQVDGTIVTPDGPESWPSNISKRQWLVFYRVNGMALK 193

Query: 183 GNGLIDGRGQKWWDLPCKPH----KGKTMTGPCDSPIAIRFFMSSELRVEGVRIKDSPQF 242
           G G+IDGRGQKWWDLPCKPH    K   +TGPCDSPIA+RFFMSS LRVEG++IK+SPQF
Sbjct: 194 GEGVIDGRGQKWWDLPCKPHRSVNKSAIVTGPCDSPIALRFFMSSNLRVEGLQIKNSPQF 253

Query: 243 HFRFDNCKDVYIDSLHITSPALSPNTDGIHIENTHGVQIFNSIVANGDDCVSIGSGSFNV 302
           HFRFD C+ V+++SLHIT+P LSPNTDGIHIEN++ V I+NSI++NGDDCVSIGSGS++V
Sbjct: 254 HFRFDGCQGVHVESLHITAPPLSPNTDGIHIENSNSVTIYNSIISNGDDCVSIGSGSYDV 313

Query: 303 EIRNITCGP-GHGISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGFGTVRGV 362
           +IRN+TCGP GHGISIGSLGNHNSRACVSNITVRDS+IK SDNGVRIKTWQGG G+V GV
Sbjct: 314 DIRNLTCGPGGHGISIGSLGNHNSRACVSNITVRDSVIKYSDNGVRIKTWQGGSGSVSGV 373

Query: 363 SFNNINMDNVRNPIIIDQFYCLNKGCLNQTSAVVVSDISYIDIKGTYDIRSPPMHFGCSD 422
           +FNNI++D+VRNPIIIDQ+YC+ K C N+TSAV VSDI+Y  IKGTYDIRSPPMHFGCSD
Sbjct: 374 TFNNIHVDSVRNPIIIDQYYCMTKDCANKTSAVFVSDIAYQGIKGTYDIRSPPMHFGCSD 433

Query: 423 SMPCMNLTLANIELFPSRGDILLDPFCWNAYGNLQTLTIPPISCLLETSSRSFLESEKDY 470
           ++PC NLTL++IEL P++G+I+LDPFCWNAYG  + L+IPP+ CL+    +    S  D 
Sbjct: 434 AVPCTNLTLSDIELLPAKGEIVLDPFCWNAYGIAEELSIPPVWCLMSDPPKGLQGSLVDK 488

BLAST of CSPI03G29190 vs. TAIR 10
Match: AT4G01890.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 562.4 bits (1448), Expect = 3.5e-160
Identity = 265/442 (59.95%), Postives = 333/442 (75.34%), Query Frame = 0

Query: 34  HFTISQPPSPSPS-SEPAGSPPEDNGGPPSSSINSTSIFDVRHFGAIGDGTADDTAAFKM 93
           H   SQPPSPSP+ ++P+ SP        S  ++   ++DVR +GA+G+G ADDT +FK 
Sbjct: 33  HHQASQPPSPSPNPNDPSKSPSR------SQDLDHEVVYDVRKYGAVGNGVADDTVSFKT 92

Query: 94  AWDTACQRNDDDNNDDYGVILVPYGFSFMIQSTIFTGPCKNGVVFQVDGTLMAPDGPDAW 153
           AWD+AC  N    N+   V+ VPYGF+FMI+STIFTGPC++   FQVDGT++  DGP +W
Sbjct: 93  AWDSACSNN---KNNTASVLHVPYGFTFMIRSTIFTGPCRSYQYFQVDGTIVPRDGPKSW 152

Query: 154 PKGYSAHQWLVFYRVYNMSLQGNGLIDGRGQKWWDLPCKPHK---GKT-MTGPCDSPIAI 213
           P   +  QWL FYR+  M+LQG G+IDGRGQ WWDLPCKPH+    KT + GPC+SP A+
Sbjct: 153 PSSLNKRQWLAFYRINGMALQGAGVIDGRGQNWWDLPCKPHQQNVNKTKLAGPCESPAAL 212

Query: 214 RFFMSSELRVEGVRIKDSPQFHFRFDNCKDVYIDSLHITSPALSPNTDGIHIENTHGVQI 273
           RFFMSS + V+G+ IK+SPQ H + D C  V+I+SL I SP  SPNTDGIHIEN++ V+I
Sbjct: 213 RFFMSSNVIVKGLSIKNSPQVHLKLDGCHVVHINSLRIISPPASPNTDGIHIENSNSVEI 272

Query: 274 FNSIVANGDDCVSIGSGSFNVEIRNITCGP-GHGISIGSLGNHNSRACVSNITVRDSIIK 333
           +NS+++NGDDCVSIG G+++++IRNITCGP GHGISIGSLG  NS ACVSN+TVRDS IK
Sbjct: 273 YNSVISNGDDCVSIGPGAYDIDIRNITCGPGGHGISIGSLGEKNSHACVSNVTVRDSFIK 332

Query: 334 VSDNGVRIKTWQGGFGTVRGVSFNNINMDNVRNPIIIDQFYCLNKGCLNQTSAVVVSDIS 393
            S+NGVRIKTWQGG G+V GV+F+NI++D VRNPIIIDQ+YC  K C N+TSAV V+DI 
Sbjct: 333 FSENGVRIKTWQGGSGSVSGVTFDNIHVDTVRNPIIIDQYYCTTKSCANKTSAVFVNDIV 392

Query: 394 YIDIKGTYDIRSPPMHFGCSDSMPCMNLTLANIELFPSRGDILLDPFCWNAYGNLQTLTI 453
           Y  IKGTYDIRSPPMHFGCS+++PC NLTL+NIEL PS+ DI++ PFCWNAYG     ++
Sbjct: 393 YQSIKGTYDIRSPPMHFGCSNNVPCTNLTLSNIELLPSKEDIVVGPFCWNAYGITDEFSV 452

Query: 454 PPISCLLETSSRSFLESEKDYC 470
           P ISCL    S S L      C
Sbjct: 453 PLISCLKSNPSTSLLSGLSGRC 465

BLAST of CSPI03G29190 vs. TAIR 10
Match: AT1G56710.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 542.7 bits (1397), Expect = 2.8e-154
Identity = 250/417 (59.95%), Postives = 320/417 (76.74%), Query Frame = 0

Query: 42  SPSPSSEPAGSPPEDNGGPPSSSINSTSIFDVRHFGAIGDGTADDTAAFKMAWDTACQRN 101
           SPSP+  PA +   DN  P        ++FDV  FGAIGD + DDT+AFKMAWD AC   
Sbjct: 28  SPSPAPNPAYN-DNDNIAP--------TVFDVTSFGAIGDCSTDDTSAFKMAWDAACMST 87

Query: 102 DDDNNDDYGVILVPYGFSFMIQSTIFTGPCKNGVVFQVDGTLMAPDGPDAWPKGYSAHQW 161
              +     ++LVPY F F+++ T F GPC+  +V Q+DG +++PDGP +WP  Y   QW
Sbjct: 88  GPKS----ALLLVPYTFCFLVKPTTFNGPCRTNLVLQIDGFIVSPDGPRSWPSNYQ-RQW 147

Query: 162 LVFYRVYNMSLQGNGLIDGRGQKWWDLPCKPHK---GKTMTGPCDSPIAIRFFMSSELRV 221
           ++FYRV  +S+QG+G+I+GRGQKWW+LPCKPHK   G T TGPCDSP+AIR F SS++R+
Sbjct: 148 MMFYRVNGLSIQGSGVINGRGQKWWNLPCKPHKGLNGTTQTGPCDSPVAIRLFQSSKVRI 207

Query: 222 EGVRIKDSPQFHFRFDNCKDVYIDSLHITSPALSPNTDGIHIENTHGVQIFNSIVANGDD 281
           +G+   +S QFH RFDNC DV +DS+ I +PA SPNTDGIHIENTH VQI NS+++NGDD
Sbjct: 208 QGINFMNSAQFHVRFDNCSDVVVDSVIIKAPASSPNTDGIHIENTHNVQIRNSMISNGDD 267

Query: 282 CVSIGSGSFNVEIRNITCGPGHGISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTW 341
           C+SIG+G FNV+I+N+TCGP HGISIGSLG HNS+A VSNITV +S I  SDNGVRIKTW
Sbjct: 268 CISIGAGCFNVDIKNVTCGPSHGISIGSLGVHNSQAYVSNITVTNSTIWNSDNGVRIKTW 327

Query: 342 QGGFGTVRGVSFNNINMDNVRNPIIIDQFYCLNKGCLNQTSAVVVSDISYIDIKGTYDIR 401
           QGG G+V  + F+NI M NVRNPI+IDQ+YC    C NQTSAV++SD+ Y +IKGTYD+R
Sbjct: 328 QGGSGSVSRIVFSNILMVNVRNPIMIDQYYCQTNNCANQTSAVIISDVLYANIKGTYDLR 387

Query: 402 SPPMHFGCSDSMPCMNLTLANIELFPSRGDILLDPFCWNAYGNLQTLTIPPISCLLE 456
           SPP+HFGCSDS+PC NLTL  ++LFPS+G  L +PFCWNAYG+++ +T+PP+ CLL+
Sbjct: 388 SPPIHFGCSDSVPCTNLTLTEVDLFPSKGQHLENPFCWNAYGSMK-ITVPPVYCLLD 429

BLAST of CSPI03G29190 vs. TAIR 10
Match: AT1G48100.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 522.3 bits (1344), Expect = 4.0e-148
Identity = 248/464 (53.45%), Postives = 327/464 (70.47%), Query Frame = 0

Query: 4   IIFFFCLLFPVHGRWHNHAKKHKQHSHHHDH-FTISQPPSPSPSSEPAGSP------PED 63
           I+  + +   + G + N A+  + H H   H  T   PP P P  E A  P      P  
Sbjct: 7   ILVIYIISTVLFGHFTNVARA-RYHYHKGRHGVTHPLPPPPPPPLETANPPDQVPSDPYP 66

Query: 64  NGGPPSSSINSTSIFDVRHFGAIGDGTADDTAAFKMAWDTACQRNDDDNNDDYGVILVPY 123
           +  P     +S  +FDV  FGA+GDG+ DDTAAF+ AW  AC         + GV+L P 
Sbjct: 67  SPDPAPGDSDSGCVFDVTSFGAVGDGSCDDTAAFQDAWKAACA-------VESGVVLAPE 126

Query: 124 GFSFMIQSTIFTGPCKNGVVFQVDGTLMAPDGPDAWPKGYSAHQWLVFYRVYNMSLQGNG 183
           G  F I STIF+GPCK G+VFQ+DG LM PDGP+ WP+  + +QWLVFYR+   +  G G
Sbjct: 127 GGVFKITSTIFSGPCKPGLVFQLDGVLMPPDGPEEWPEKDNKNQWLVFYRLDGFTFSGKG 186

Query: 184 LIDGRGQKWWDLPCKPHK---GKTMTGPCDSPIAIRFFMSSELRVEGVRIKDSPQFHFRF 243
            ++G GQKWWDLPCKPH+   G + +GPC SP  IRFFMS+ + V+G+RI++SPQFH +F
Sbjct: 187 TVEGNGQKWWDLPCKPHRGPDGSSSSGPCASPTMIRFFMSNNIEVKGLRIQNSPQFHMKF 246

Query: 244 DNCKDVYIDSLHITSPALSPNTDGIHIENTHGVQIFNSIVANGDDCVSIGSGSFNVEIRN 303
           D C+ V I+ + I+SP LSPNTDGIH+ NT  V I+NS+V+NGDDC+SIG+G  +V+I+ 
Sbjct: 247 DGCQGVLINEIQISSPKLSPNTDGIHLGNTRSVGIYNSVVSNGDDCISIGTGCSDVDIQG 306

Query: 304 ITCGPGHGISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGFGTVRGVSFNNI 363
           +TCGP HGISIGSLG HNS+ACVSNITVR+++I+ SDNG+R+KTWQGG G+V  + F NI
Sbjct: 307 VTCGPSHGISIGSLGVHNSQACVSNITVRNTVIRDSDNGLRVKTWQGGTGSVSNLLFENI 366

Query: 364 NMDNVRNPIIIDQFYCLNKGCLNQTSAVVVSDISYIDIKGTYDIRSPPMHFGCSDSMPCM 423
            M+NV N II+DQ+YC +K C N+TSAV V D+ Y +IKGTYD+RSPP+HF CSD++ C 
Sbjct: 367 QMENVLNCIIVDQYYCQSKDCRNETSAVKVFDVEYRNIKGTYDVRSPPIHFACSDTVACT 426

Query: 424 NLTLANIELFPSRGDILLDPFCWNAYGNLQTLTIPPISCLLETS 458
           N+T++ +EL P  G+++ DPFCWNAYG  +TLTIPPI CLL+ S
Sbjct: 427 NITMSEVELLPEEGELVDDPFCWNAYGKQETLTIPPIDCLLDGS 462

BLAST of CSPI03G29190 vs. TAIR 10
Match: AT5G14650.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 382.5 bits (981), Expect = 4.9e-106
Identity = 191/406 (47.04%), Postives = 258/406 (63.55%), Query Frame = 0

Query: 67  STSIFDVRHFGAIGDGTADDTAAFKMAWDTACQRNDDDNNDDYGVILVPYGFSFMIQSTI 126
           S+   +V   GA GDGT+DDT AF+ AW  AC+            +LVP G +F++    
Sbjct: 47  SSGTINVLDHGAKGDGTSDDTKAFEDAWQVACK-------VAASTLLVPSGSTFLVGPVS 106

Query: 127 FTG-PCKNGVVFQVDGTLMAPDGPDAWPKGYSAHQWLVFYRVYNMSLQGNGLIDGRGQKW 186
           F G  CK  +VFQ++G ++AP    AW  G    QW+ F  +  ++++G G+IDGRG  W
Sbjct: 107 FLGKECKEKIVFQLEGKIIAPTSASAWGSGLL--QWIEFKALQGITIKGKGIIDGRGSVW 166

Query: 187 WDLPCKPHKGKTMTGPCDSPIAIRFFMSSELRVEGVRIKDSPQFHFRFDNCKDVYIDSLH 246
           W+       G  M  P   P A+RF+ S+ + V G+ I++SPQ H +FDNC  + +    
Sbjct: 167 WN----DMMGTKM--PRTKPTALRFYGSNGVTVSGITIQNSPQTHLKFDNCISIQVSDFT 226

Query: 247 ITSPALSPNTDGIHIENTHGVQIFNSIVANGDDCVSIGSGSFNVEIRNITCGPGHGISIG 306
            +SP  SPNTDGIH++N+    I+ S +A GDDC+SI +G  N+ I ++ CGPGHGISIG
Sbjct: 227 TSSPGDSPNTDGIHLQNSQDAVIYRSTLACGDDCISIQTGCSNINIHDVDCGPGHGISIG 286

Query: 307 SLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGFGTVRGVSFNNINMDNVRNPIIID 366
            LG  N++ACVSNITVRD  +  + NGVRIK+WQGG G+V+ V F+NI + NV NPIIID
Sbjct: 287 GLGKDNTKACVSNITVRDVTMHETTNGVRIKSWQGGSGSVKQVMFSNIQVSNVANPIIID 346

Query: 367 QFYCLNKGCLNQTSAVVVSDISYIDIKGTYDIRSPPMHFGCSDSMPCMNLTLANIELFPS 426
           Q+YC   GC N+TSAV VS+I+YI+IKGTY     P+ F CSDS+PC  ++L+ IEL P+
Sbjct: 347 QYYCDGGGCHNETSAVAVSNINYINIKGTY--TKEPVRFACSDSLPCTGISLSTIELKPA 406

Query: 427 RGDI-LLDPFCWNAYGNLQTLTIPPISCL-LETSSRSFLESEKDYC 470
            G    LDPFCW A+G L+T T+PPI CL  E S  +   S  D C
Sbjct: 407 TGKASSLDPFCWKAHGELKTKTLPPIQCLKTEKSPEAASRSNNDAC 435

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q949Z15.6e-14753.45Polygalacturonase At1g48100 OS=Arabidopsis thaliana OX=3702 GN=At1g48100 PE=2 SV... [more]
Q94AJ51.4e-9243.32Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g8017... [more]
P432128.2e-8239.75Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1[more]
Q020961.4e-8143.55Polygalacturonase OS=Persea americana OX=3435 PE=2 SV=1[more]
Q8RY292.4e-8142.70Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A0A0LEQ61.0e-28499.57Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G664600 PE=3 SV=1[more]
A0A5D3DA936.6e-26892.93Polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold237G00... [more]
A0A5A7T9244.8e-25890.64Polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold36G003... [more]
A0A1S4DWP41.2e-24595.55polygalacturonase At1g48100 OS=Cucumis melo OX=3656 GN=LOC103490076 PE=3 SV=1[more]
A0A6J1FAK71.9e-23883.09polygalacturonase At1g48100-like OS=Cucurbita moschata OX=3662 GN=LOC111442323 P... [more]
Match NameE-valueIdentityDescription
XP_031737994.12.1e-28499.57polygalacturonase At1g48100 [Cucumis sativus] >KGN58717.2 hypothetical protein C... [more]
TYK20501.11.4e-26792.93polygalacturonase [Cucumis melo var. makuwa][more]
KAA0038107.19.8e-25890.64polygalacturonase [Cucumis melo var. makuwa][more]
XP_038905655.11.9e-25388.56polygalacturonase At1g48100-like [Benincasa hispida][more]
XP_016900401.12.5e-24595.55PREDICTED: polygalacturonase At1g48100, partial [Cucumis melo][more]
Match NameE-valueIdentityDescription
AT1G02460.11.0e-18062.79Pectin lyase-like superfamily protein [more]
AT4G01890.13.5e-16059.95Pectin lyase-like superfamily protein [more]
AT1G56710.12.8e-15459.95Pectin lyase-like superfamily protein [more]
AT1G48100.14.0e-14853.45Pectin lyase-like superfamily protein [more]
AT5G14650.14.9e-10647.04Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006626Parallel beta-helix repeatSMARTSM00710pbh1coord: 316..337
e-value: 20.0
score: 14.1
coord: 263..284
e-value: 1100.0
score: 6.9
coord: 236..262
e-value: 550.0
score: 9.0
coord: 286..306
e-value: 2600.0
score: 4.3
coord: 345..366
e-value: 4200.0
score: 2.8
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 112..422
e-value: 8.8E-89
score: 297.8
IPR000743Glycoside hydrolase, family 28PROSITEPS00502POLYGALACTURONASEcoord: 293..306
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 61..463
e-value: 3.1E-115
score: 387.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 42..56
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 23..64
NoneNo IPR availablePANTHERPTHR31375FAMILY NOT NAMEDcoord: 3..459
NoneNo IPR availablePANTHERPTHR31375:SF119POLYGALACTURONASE/GLYCOSIDE HYDROLASE FAMILY PROTEINcoord: 3..459
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 65..455

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI03G29190.1CSPI03G29190.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004650 polygalacturonase activity