CSPI03G29070 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI03G29070
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Descriptioneukaryotic initiation factor 4A-III
LocationChr3: 26592006 .. 26596447 (+)
RNA-Seq ExpressionCSPI03G29070
SyntenyCSPI03G29070
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GGATAGGAATAGGATATAGCCTCGTACTTAATTCAATCTCTCTCTTTTTCTTCTTCCAACTTTCCTTGTCGATCAATAATCGTTCATCAGCCCTAATCTGCGGTTTCGTCTCCATCTATATCTTCCTCTCGCCGGGAATTTTCCGGTTAGTCAAGTCCTCAGTCGCGATCTCGGCAACTACCTCAACCGAACATGGCGGCTGCAGCTACAACGAGCGTTGTGCCACCGAATCGGCGTCGGACGATTAGCGCGCCTGAAGACAAATTGGAATTCGAGACGACGGAGGGGGTTGAGCCGATCTTGACTTTCGATCAGATGGGCATTAAAGATGACCTTCTTCGTGGAATATATGCCTACGGTTTTGAAAAGCCATCTGCCATTCAGCAGAGAGCTGTGAGGCCGATTATTGAGGGTCGAGATGTTATTGCGCAGGCTCAATCTGGGACTGGGAAGACTTCCATGATTGCCCTTACCGTTTGCCAGATGGTTGATACCACAAGTAGAGAGTGAGTGTTGTTTACCCTATTGAATATTCAGTGTTTCTCATCTTCTTCCCCCTCTATATCTTTCTATTCTGTGCATTTTGTGTTCTTTGTCGCTTCTATTTCTTTATGCAATGTTTTGTGCAAATTATATGGAAGTTGTGTCGTAACCTAGCTCAGAAATTCAATTGGAAAGGCAAAAATTGGTTGGATGTTAGTTGGTTTTTTTCTACAAGAAGTTGTTATAACCTCATCTCGACGTTTTGGAAAGTTTGCAAGGAAGTTAAAGTTTTGAATATGCATTCAGCTCATGGTTCAGGAGTTTTTTCTAATTTACATCTGATCTTTTTGGAATGCCATCTTTTTAACTTCAAATTCTTATGATCATTATGCATCTTATATCTAATCAGGGTGCAGGCCTTGATCTTGTCCCCTACGAGAGAATTGGCTACTCAGACAGAGAAGGTTATATTGGCCATTGGCGACTACATTAATATACAAGCACACGCATGTATTGGAGGCAAAAGTGTGGGTGAAGATATCAGAAAGCTTGAGTTTGGAGTTCAGGTTGTGTCGGGAACTCCTGGAAGAGTCTGTGACATGATCAAGAGAAGGACATTGCGTACCAGAGCCATTAAGTTATTAGTTTTGGTAAGTTTGGGTGCCGTTCAATCACTCTCATGTAGTAGTACAATTATCGTACTTAATTTTTTTATGCAACTTTCTATACGGTATCATTTCATTTTCTAATATTTACTTCTTCAGGACGAGTCTGATGAAATGCTGAGCAGGGGGTTTAAAGACCAAATTTATGATGTATATCGTTATCTTCCACCAGAACTTCAGGTTCTCTCTTTCTCTCCCTTTCTCTTCACTTCAATGTGTAAATGATTGTGCCCATGAGAGGCAGAAGTTGATAAAAAAGTAATGTGAGTATACATGCTTTTCATACATATGATGACAAACGTGTATGTTCAATATTTTTTTCCTGCAATAATACCATACGTGATTGATTTATTAATCAGGACTATTTTCCTGAATGGTGGTTTTGTGGTTTCTGGGAAATGCTCAAAGTGGCAGACATATAATCTTGTCTAACTTCATTATAACTTTAGATTTTTATTTTCATTTATTTCCTTCACATGCCATTTTGTAACAGATGTCATATTAATAATATATTCCTGTTTATATGGGTTTTCTAATATTTGTAGGGTATATTTAGTTGTTGCCCCTTTTCGTGTTTGGTTATGGGTGATTAGTGATTTTTACCTCTTTCTGTGTAGTTTTCTATTTTTACATGTTTTTATCGAATCTAATGAGTTTTCCCCTCAATTTTAATTTTAGGTTGTCTTGATTTCTGCTACCCTTCCTCATGAAATCCTGGAGATGACAAACAAGTTTATGACAGATCCTGTGAGGATTCTTGTCAAGCGTGATGAGTTGACTTTGGAGGTTTGTAACCTTTGTTGTTGTATCTGACTAATTCCCTCTGCATATATATCAGTGCCCTGATGCATGCTTTTATATTGACAGGGTATTAAGCAATTCTTTGTTGCGGTTGAAAGGGAAGAGTGGAAGTTTGATACCTTATGTGATCTTTATGATACCTTGACTATCACTCAAGCTGTCATTTTCTGTAACACTAAGCGGAAGGTAACAATGGGGTTGATGAATTCTTTTTGTAGATCTTCAAATTTTTTTTTCTCAAAGTCTTGTCAGATTCATCCTGAAACAGCAAATATTGTAACGTCGGCCTTTTTATACATGTTTGCCTTTATATGTTAATTTTGGTGTGTCCCAGAATCTAAATGTTTGAAATATAGTTTTATTATTACTTAACCTTTGTCCTCACCCTTTTTTACTGCTTTAGTATAAATTTGCCTGTCTACAGTATGCCCATGCTTAGAAAAGTTTATTTGAGGAAAGGATGGGATAGGATTATCTTTTTGTTAAGTAGGAAGAGAGCGTTCCGTTACTTGAGGGTAATCCCCCAGATTCTTTATCCTCATTACACCTGGTTATAATCCATATAGTACATAATACCCACATTCTACCACACCTTCTTCCAAATACTTGCTGAGGATCACACTGAAGCAAATTGTTGGTTACAATGGGTCCTACACACTCTATCGCAACTACTTATCAGACCTATCTCTTGCCCTTTTCCTACTATGTGCTTCTAACAACTGGAGGAGGGCTATCTCAGCCGTATATCTCATTGTACTGGTTCTTGGATGGCTAGCCCATGGCCTTTTTGTAATGTCTTTCTTTTTGTACTTTCATACTATCAATGAATTTGTTTTTATAAAAAAACTTATCCATGTTTACCAAAAATAATAATTTTGCGGTGGAATAGCTTCATGCATTTTGAATATCTTGTGTGATCTTTTGCTCCCAGGTTGAATGGTTGACTGAAAAGATGCGAAGTAATAACTTCACAGTTTCACATATGCATGGGGACATGCCTCAAAAGGAAAGAGATGCAATCATGGGAGAGTTCCGATCAGGAACTACCCGTGTTCTAATTACTACTGATGTTTGGGCACGAGGGCTTGATGTGCAGCAGGCAAGTTTCTGGTTCTTTGATTTTATTGATCTTTGTTGATGTGCCCCCTTACAAATCCAAGCAGCTCTAGTTGTAGTTGTCTTAGGAAATTCTAACTTGGTATTAAATGAAAGTAAAATGAATAAAAGGATAGGATATTCTGATCAAGTAGTCCATTTTCTCTATGTTTTTGTCCGGACTCCTTAGAGTTGGGTTGATTCCTCTCAATAATGTCAGTAAAATTATTGTTAACAGATTTGAAGTTGTGTGAAATGACCATGGTGATTATTTCCATGTACTGGGGAGTTACTGGACAATCTTTTTCTATTCTCTGGAAGATATTAGAAAAATATCTTTAGATGGTGATATCTAGAGAAGACTGGGTATTAAATTTTGTAAGGCTAAAAATAGATTACTTCCAAGTTTACATTATCAATTGGTCCGCCCACCAAACCATCTCTGTAAACCTCCTACTATTTGAATTTTGAACTCCCACTCATTGTTAAAATCTATGTTTATTTTTCATTCAGTACTTACTGTGGCAATTATCCAAAAATCTACATCTTATGCTTATTTGCATCGACTTTTATCAGGTATCTCTTGTGATAAATTATGATCTTCCAAACAATCGAGAACTTTACATTCACAGGATAGGAAGGTCGGGTCGGTTTGGGCGCAAGGTATGTAACTTTATTCCATATATCAGTTGATTTCCTAGTATTCCTCACACTAAACTGTATTTCAATGTTTCCTTTGATGAAAGCTATGTTTATGATTCCTTTAGATGGAAAGCTAAGTTAACTCAAAATAAAATATCCGACCTTTTGAAAAGTTATATGATAGGATGTATTTTAATGCAAAAAATTGTGGGATAACGCAGGGTGTTGCTATTAACTTTGTGAAAAGCGATGACATCAAGATCCTGAGAGATATTGAGCAATACTACAGTACCCAGATTGACGAGATGCCCATGAATGTTGCTGATTTGATTTAACACTTGCTTGAATTGCCTGCTGTTGTATGCTGTGGAAGAATTGTGTTAATATATGCTCTTGCACTTGAGGTTTTGTGTCTTTTTCATTGGTGATATGAAAATGATGTAGCTTAGGAAGTTTTAACGACCTTTTTTCTGATGAGTGGCTTGGATATGTCCATAAATTGTATAATGCCTGCGATTGTTTTTTTTGTTCATTTTTTCAGTGGGGAAGGAACTCAATATTATATTTCTGATCGACTCGAAATATATTATTCTGAGATTAGTTGCGTTGTGCCTCCTATGTCCATTTTTCTCTCTCTAGATTTGGGCATACCTTTCTTTTGGACATCTCTATTGAATTTTTTTCTTTCAGGTTGAAGTAGAAAATACATTTGAAGTTTTAAAAGTTTGATACCTTTTAAACTTTCCTATACGTGGG

mRNA sequence

GGATAGGAATAGGATATAGCCTCGTACTTAATTCAATCTCTCTCTTTTTCTTCTTCCAACTTTCCTTGTCGATCAATAATCGTTCATCAGCCCTAATCTGCGGTTTCGTCTCCATCTATATCTTCCTCTCGCCGGGAATTTTCCGGTTAGTCAAGTCCTCAGTCGCGATCTCGGCAACTACCTCAACCGAACATGGCGGCTGCAGCTACAACGAGCGTTGTGCCACCGAATCGGCGTCGGACGATTAGCGCGCCTGAAGACAAATTGGAATTCGAGACGACGGAGGGGGTTGAGCCGATCTTGACTTTCGATCAGATGGGCATTAAAGATGACCTTCTTCGTGGAATATATGCCTACGGTTTTGAAAAGCCATCTGCCATTCAGCAGAGAGCTGTGAGGCCGATTATTGAGGGTCGAGATGTTATTGCGCAGGCTCAATCTGGGACTGGGAAGACTTCCATGATTGCCCTTACCGTTTGCCAGATGGTTGATACCACAAGTAGAGAGGTGCAGGCCTTGATCTTGTCCCCTACGAGAGAATTGGCTACTCAGACAGAGAAGGTTATATTGGCCATTGGCGACTACATTAATATACAAGCACACGCATGTATTGGAGGCAAAAGTGTGGGTGAAGATATCAGAAAGCTTGAGTTTGGAGTTCAGGTTGTGTCGGGAACTCCTGGAAGAGTCTGTGACATGATCAAGAGAAGGACATTGCGTACCAGAGCCATTAAGTTATTAGTTTTGGACGAGTCTGATGAAATGCTGAGCAGGGGGTTTAAAGACCAAATTTATGATGTATATCGTTATCTTCCACCAGAACTTCAGGTTGTCTTGATTTCTGCTACCCTTCCTCATGAAATCCTGGAGATGACAAACAAGTTTATGACAGATCCTGTGAGGATTCTTGTCAAGCGTGATGAGTTGACTTTGGAGGGTATTAAGCAATTCTTTGTTGCGGTTGAAAGGGAAGAGTGGAAGTTTGATACCTTATGTGATCTTTATGATACCTTGACTATCACTCAAGCTGTCATTTTCTGTAACACTAAGCGGAAGGTTGAATGGTTGACTGAAAAGATGCGAAGTAATAACTTCACAGTTTCACATATGCATGGGGACATGCCTCAAAAGGAAAGAGATGCAATCATGGGAGAGTTCCGATCAGGAACTACCCGTGTTCTAATTACTACTGATGTTTGGGCACGAGGGCTTGATGTGCAGCAGGTATCTCTTGTGATAAATTATGATCTTCCAAACAATCGAGAACTTTACATTCACAGGATAGGAAGGTCGGGTCGGTTTGGGCGCAAGGGTGTTGCTATTAACTTTGTGAAAAGCGATGACATCAAGATCCTGAGAGATATTGAGCAATACTACAGTACCCAGATTGACGAGATGCCCATGAATGTTGCTGATTTGATTTAACACTTGCTTGAATTGCCTGCTGTTGTATGCTGTGGAAGAATTGTGTTAATATATGCTCTTGCACTTGAGGTTTTGTGTCTTTTTCATTGGTGATATGAAAATGATGTAGCTTAGGAAGTTTTAACGACCTTTTTTCTGATGAGTGGCTTGGATATGTCCATAAATTGTATAATGCCTGCGATTGTTTTTTTTGTTCATTTTTTCAGTGGGGAAGGAACTCAATATTATATTTCTGATCGACTCGAAATATATTATTCTGAGATTAGTTGCGTTGTGCCTCCTATGTCCATTTTTCTCTCTCTAGATTTGGGCATACCTTTCTTTTGGACATCTCTATTGAATTTTTTTCTTTCAGGTTGAAGTAGAAAATACATTTGAAGTTTTAAAAGTTTGATACCTTTTAAACTTTCCTATACGTGGG

Coding sequence (CDS)

ATGGCGGCTGCAGCTACAACGAGCGTTGTGCCACCGAATCGGCGTCGGACGATTAGCGCGCCTGAAGACAAATTGGAATTCGAGACGACGGAGGGGGTTGAGCCGATCTTGACTTTCGATCAGATGGGCATTAAAGATGACCTTCTTCGTGGAATATATGCCTACGGTTTTGAAAAGCCATCTGCCATTCAGCAGAGAGCTGTGAGGCCGATTATTGAGGGTCGAGATGTTATTGCGCAGGCTCAATCTGGGACTGGGAAGACTTCCATGATTGCCCTTACCGTTTGCCAGATGGTTGATACCACAAGTAGAGAGGTGCAGGCCTTGATCTTGTCCCCTACGAGAGAATTGGCTACTCAGACAGAGAAGGTTATATTGGCCATTGGCGACTACATTAATATACAAGCACACGCATGTATTGGAGGCAAAAGTGTGGGTGAAGATATCAGAAAGCTTGAGTTTGGAGTTCAGGTTGTGTCGGGAACTCCTGGAAGAGTCTGTGACATGATCAAGAGAAGGACATTGCGTACCAGAGCCATTAAGTTATTAGTTTTGGACGAGTCTGATGAAATGCTGAGCAGGGGGTTTAAAGACCAAATTTATGATGTATATCGTTATCTTCCACCAGAACTTCAGGTTGTCTTGATTTCTGCTACCCTTCCTCATGAAATCCTGGAGATGACAAACAAGTTTATGACAGATCCTGTGAGGATTCTTGTCAAGCGTGATGAGTTGACTTTGGAGGGTATTAAGCAATTCTTTGTTGCGGTTGAAAGGGAAGAGTGGAAGTTTGATACCTTATGTGATCTTTATGATACCTTGACTATCACTCAAGCTGTCATTTTCTGTAACACTAAGCGGAAGGTTGAATGGTTGACTGAAAAGATGCGAAGTAATAACTTCACAGTTTCACATATGCATGGGGACATGCCTCAAAAGGAAAGAGATGCAATCATGGGAGAGTTCCGATCAGGAACTACCCGTGTTCTAATTACTACTGATGTTTGGGCACGAGGGCTTGATGTGCAGCAGGTATCTCTTGTGATAAATTATGATCTTCCAAACAATCGAGAACTTTACATTCACAGGATAGGAAGGTCGGGTCGGTTTGGGCGCAAGGGTGTTGCTATTAACTTTGTGAAAAGCGATGACATCAAGATCCTGAGAGATATTGAGCAATACTACAGTACCCAGATTGACGAGATGCCCATGAATGTTGCTGATTTGATTTAA

Protein sequence

MAAAATTSVVPPNRRRTISAPEDKLEFETTEGVEPILTFDQMGIKDDLLRGIYAYGFEKPSAIQQRAVRPIIEGRDVIAQAQSGTGKTSMIALTVCQMVDTTSREVQALILSPTRELATQTEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAIKLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILVKRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNNFTVSHMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELYIHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI*
Homology
BLAST of CSPI03G29070 vs. ExPASy Swiss-Prot
Match: P41380 (Eukaryotic initiation factor 4A-3 OS=Nicotiana plumbaginifolia OX=4092 PE=2 SV=1)

HSP 1 Score: 716.1 bits (1847), Expect = 2.3e-205
Identity = 359/389 (92.29%), Postives = 373/389 (95.89%), Query Frame = 0

Query: 22  EDKLEFETTEGVEPILTFDQMGIKDDLLRGIYAYGFEKPSAIQQRAVRPIIEGRDVIAQA 81
           ED+L FET++GVEPI +F +MGIKDDLLRG+Y YGFEKPSAIQQRAV PII GRDVIAQA
Sbjct: 3   EDRLVFETSKGVEPIASFAEMGIKDDLLRGVYQYGFEKPSAIQQRAVLPIISGRDVIAQA 62

Query: 82  QSGTGKTSMIALTVCQMVDTTSREVQALILSPTRELATQTEKVILAIGDYINIQAHACIG 141
           QSGTGKTSMIALTVCQ+VDT S EVQALILSPTRELA QTEKVILAIGDYIN+QAHACIG
Sbjct: 63  QSGTGKTSMIALTVCQIVDTKSSEVQALILSPTRELAAQTEKVILAIGDYINVQAHACIG 122

Query: 142 GKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAIKLLVLDESDEMLSRGFKDQIY 201
           GKSVGEDIRKLE GVQVVSGTPGRVCDMIKRRTLRTR IKLL+LDESDEMLSRGFKDQIY
Sbjct: 123 GKSVGEDIRKLEHGVQVVSGTPGRVCDMIKRRTLRTRGIKLLILDESDEMLSRGFKDQIY 182

Query: 202 DVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILVKRDELTLEGIKQFFVAVEREE 261
           DVYRYLPPELQVVLISATLP+EILE+T+KFMTDPVRILVKRDELTLEGIKQFFVAVE+EE
Sbjct: 183 DVYRYLPPELQVVLISATLPNEILEITSKFMTDPVRILVKRDELTLEGIKQFFVAVEKEE 242

Query: 262 WKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNNFTVSHMHGDMPQKERDAIMGE 321
           WKFDTLCDLYDTLTITQAVIFCNTKRKV+WLT KMR NNFTVS MHGDMPQKERDAIM E
Sbjct: 243 WKFDTLCDLYDTLTITQAVIFCNTKRKVDWLTSKMRENNFTVSSMHGDMPQKERDAIMAE 302

Query: 322 FRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELYIHRIGRSGRFGRKGVAINFVK 381
           FR GTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELYIHRIGRSGRFGRKGVAINFVK
Sbjct: 303 FRGGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELYIHRIGRSGRFGRKGVAINFVK 362

Query: 382 SDDIKILRDIEQYYSTQIDEMPMNVADLI 411
           SDDIKILRDIEQYYSTQIDEMPMNVADLI
Sbjct: 363 SDDIKILRDIEQYYSTQIDEMPMNVADLI 391

BLAST of CSPI03G29070 vs. ExPASy Swiss-Prot
Match: Q5VNM3 (Eukaryotic initiation factor 4A-III homolog A OS=Oryza sativa subsp. japonica OX=39947 GN=EIF4A3A PE=1 SV=1)

HSP 1 Score: 708.4 bits (1827), Expect = 4.8e-203
Identity = 359/408 (87.99%), Postives = 380/408 (93.14%), Query Frame = 0

Query: 3   AAATTSVVPPNRRRTISAPEDKLEFETTEGVEPILTFDQMGIKDDLLRGIYAYGFEKPSA 62
           AAATTS     RR   +  ++ L FET+ GVE I +FDQMGI+DDLLRGIYAYGFEKPSA
Sbjct: 2   AAATTS-----RRGPGAMDDENLTFETSPGVEVISSFDQMGIRDDLLRGIYAYGFEKPSA 61

Query: 63  IQQRAVRPIIEGRDVIAQAQSGTGKTSMIALTVCQMVDTTSREVQALILSPTRELATQTE 122
           IQQRAV PII GRDVIAQAQSGTGKTSMI+L+VCQ+VDT  REVQALILSPTRELA QTE
Sbjct: 62  IQQRAVLPIISGRDVIAQAQSGTGKTSMISLSVCQIVDTAVREVQALILSPTRELAAQTE 121

Query: 123 KVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAIKL 182
           +V+LAIGD+INIQ HACIGGKS+GEDIRKLE GV VVSGTPGRVCDMIKRRTLRTRAIKL
Sbjct: 122 RVMLAIGDFINIQVHACIGGKSIGEDIRKLEHGVHVVSGTPGRVCDMIKRRTLRTRAIKL 181

Query: 183 LVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILVKR 242
           L+LDE+DEML RGFKDQIYDVYRYLPPELQV LISATLPHEILEMT+KFMTDPVRILVKR
Sbjct: 182 LILDEADEMLGRGFKDQIYDVYRYLPPELQVCLISATLPHEILEMTSKFMTDPVRILVKR 241

Query: 243 DELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNNFT 302
           DELTLEGIKQFFVAVE+EEWKFDTLCDLYDTLTITQAVIFCNTKRKV+WLTE+MRSNNFT
Sbjct: 242 DELTLEGIKQFFVAVEKEEWKFDTLCDLYDTLTITQAVIFCNTKRKVDWLTERMRSNNFT 301

Query: 303 VSHMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELYIH 362
           VS MHGDMPQKERDAIMGEFRSG TRVLITTDVWARGLDVQQVSLVINYDLPNNRELYIH
Sbjct: 302 VSAMHGDMPQKERDAIMGEFRSGATRVLITTDVWARGLDVQQVSLVINYDLPNNRELYIH 361

Query: 363 RIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI 411
           RIGRSGRFGRKGVAINFVK +DI+ILRDIEQYYSTQIDEMPMNVADLI
Sbjct: 362 RIGRSGRFGRKGVAINFVKKEDIRILRDIEQYYSTQIDEMPMNVADLI 404

BLAST of CSPI03G29070 vs. ExPASy Swiss-Prot
Match: Q10I26 (Eukaryotic initiation factor 4A-III homolog B OS=Oryza sativa subsp. japonica OX=39947 GN=EIF4A3B PE=1 SV=1)

HSP 1 Score: 708.4 bits (1827), Expect = 4.8e-203
Identity = 359/408 (87.99%), Postives = 380/408 (93.14%), Query Frame = 0

Query: 3   AAATTSVVPPNRRRTISAPEDKLEFETTEGVEPILTFDQMGIKDDLLRGIYAYGFEKPSA 62
           AAATTS     RR   +  ++ L FET+ GVE I +FDQMGI++DLLRGIYAYGFEKPSA
Sbjct: 2   AAATTS-----RRGPGAMDDENLTFETSPGVEVISSFDQMGIREDLLRGIYAYGFEKPSA 61

Query: 63  IQQRAVRPIIEGRDVIAQAQSGTGKTSMIALTVCQMVDTTSREVQALILSPTRELATQTE 122
           IQQRAV PII GRDVIAQAQSGTGKTSMI+L+VCQ+VDT  REVQALILSPTRELA QTE
Sbjct: 62  IQQRAVLPIISGRDVIAQAQSGTGKTSMISLSVCQIVDTAVREVQALILSPTRELAAQTE 121

Query: 123 KVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAIKL 182
           +V+LAIGDYINIQ HACIGGKS+GEDIRKLE GV VVSGTPGRVCDMIKRRTLRTRAIKL
Sbjct: 122 RVMLAIGDYINIQVHACIGGKSIGEDIRKLEHGVHVVSGTPGRVCDMIKRRTLRTRAIKL 181

Query: 183 LVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILVKR 242
           L+LDE+DEML RGFKDQIYDVYRYLPPELQV LISATLPHEILEMT+KFMTDPVRILVKR
Sbjct: 182 LILDEADEMLGRGFKDQIYDVYRYLPPELQVCLISATLPHEILEMTSKFMTDPVRILVKR 241

Query: 243 DELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNNFT 302
           DELTLEGIKQFFVAVE+EEWKFDTLCDLYDTLTITQAVIFCNTKRKV+WLTE+MRSNNFT
Sbjct: 242 DELTLEGIKQFFVAVEKEEWKFDTLCDLYDTLTITQAVIFCNTKRKVDWLTERMRSNNFT 301

Query: 303 VSHMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELYIH 362
           VS MHGDMPQKERDAIMGEFRSG TRVLITTDVWARGLDVQQVSLVINYDLPNNRELYIH
Sbjct: 302 VSAMHGDMPQKERDAIMGEFRSGATRVLITTDVWARGLDVQQVSLVINYDLPNNRELYIH 361

Query: 363 RIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI 411
           RIGRSGRFGRKGVAINFVK +DI+ILRDIEQYYSTQIDEMPMNVADLI
Sbjct: 362 RIGRSGRFGRKGVAINFVKKEDIRILRDIEQYYSTQIDEMPMNVADLI 404

BLAST of CSPI03G29070 vs. ExPASy Swiss-Prot
Match: Q94A52 (Eukaryotic initiation factor 4A-III homolog OS=Arabidopsis thaliana OX=3702 GN=EIF4A3 PE=1 SV=2)

HSP 1 Score: 695.7 bits (1794), Expect = 3.2e-199
Identity = 347/397 (87.41%), Postives = 376/397 (94.71%), Query Frame = 0

Query: 14  RRRTISAPEDKLEFETTEGVEPILTFDQMGIKDDLLRGIYAYGFEKPSAIQQRAVRPIIE 73
           RR   +  +DKL FETT+G+EPI +F+ MGIK+D+LRG+Y YGFEKPSAIQQRAV PI++
Sbjct: 12  RRGGGAMDDDKLVFETTDGIEPITSFNDMGIKEDVLRGVYEYGFEKPSAIQQRAVMPILQ 71

Query: 74  GRDVIAQAQSGTGKTSMIALTVCQMVDTTSREVQALILSPTRELATQTEKVILAIGDYIN 133
           GRDVIAQAQSGTGKTSMIAL+VCQ+VDT+SREVQALILSPTRELATQTEK I AIG + N
Sbjct: 72  GRDVIAQAQSGTGKTSMIALSVCQVVDTSSREVQALILSPTRELATQTEKTIQAIGLHAN 131

Query: 134 IQAHACIGGKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAIKLLVLDESDEMLS 193
           IQAHACIGG SVGEDIRKLE GV VVSGTPGRVCDMIKRR+LRTRAIKLL+LDESDEMLS
Sbjct: 132 IQAHACIGGNSVGEDIRKLEHGVHVVSGTPGRVCDMIKRRSLRTRAIKLLILDESDEMLS 191

Query: 194 RGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILVKRDELTLEGIKQF 253
           RGFKDQIYDVYRYLPP+LQV L+SATLPHEILEMT+KFMT+PV+ILVKRDELTLEGIKQF
Sbjct: 192 RGFKDQIYDVYRYLPPDLQVCLVSATLPHEILEMTSKFMTEPVKILVKRDELTLEGIKQF 251

Query: 254 FVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNNFTVSHMHGDMPQK 313
           FVAVE+EEWKFDTLCDLYDTLTITQAVIFCNTKRKV++L+EKMRS+NFTVS MHGDMPQK
Sbjct: 252 FVAVEKEEWKFDTLCDLYDTLTITQAVIFCNTKRKVDYLSEKMRSHNFTVSSMHGDMPQK 311

Query: 314 ERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELYIHRIGRSGRFGRK 373
           ERDAIM EFRSG +RVLITTDVWARG+DVQQVSLVINYDLPNNRELYIHRIGRSGRFGRK
Sbjct: 312 ERDAIMNEFRSGDSRVLITTDVWARGIDVQQVSLVINYDLPNNRELYIHRIGRSGRFGRK 371

Query: 374 GVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI 411
           GVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI
Sbjct: 372 GVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI 408

BLAST of CSPI03G29070 vs. ExPASy Swiss-Prot
Match: Q7ZVA6 (Eukaryotic initiation factor 4A-III OS=Danio rerio OX=7955 GN=eif4a3 PE=2 SV=1)

HSP 1 Score: 649.4 bits (1674), Expect = 2.7e-185
Identity = 320/404 (79.21%), Postives = 363/404 (89.85%), Query Frame = 0

Query: 7   TSVVPPNRRRTISAPEDKLEFETTEGVEPILTFDQMGIKDDLLRGIYAYGFEKPSAIQQR 66
           T+VVP  +R        K+EFET+E V+   TFD MG+++DLLRGIYAYGFEKPSAIQQR
Sbjct: 3   TAVVPVRKRILKEEDMTKIEFETSEEVDVTPTFDTMGLREDLLRGIYAYGFEKPSAIQQR 62

Query: 67  AVRPIIEGRDVIAQAQSGTGKTSMIALTVCQMVDTTSREVQALILSPTRELATQTEKVIL 126
           A++ II+GRDVIAQ+QSGTGKT+   ++V Q +D   RE QALIL+PTRELA Q +KV+L
Sbjct: 63  AIKQIIKGRDVIAQSQSGTGKTATFCVSVLQCLDIQVRETQALILAPTRELAGQIQKVLL 122

Query: 127 AIGDYINIQAHACIGGKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAIKLLVLD 186
           A+GDY+N+Q HACIGG +VGEDIRKL++G  VV+GTPGRV DMI+RR+LRTRAIK+LVLD
Sbjct: 123 ALGDYMNVQCHACIGGTNVGEDIRKLDYGQHVVAGTPGRVFDMIRRRSLRTRAIKMLVLD 182

Query: 187 ESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILVKRDELT 246
           E+DEML++GFK+QIYDVYRYLPP  QV LISATLPHEILEMTNKFMTDP+RILVKRDELT
Sbjct: 183 EADEMLNKGFKEQIYDVYRYLPPATQVCLISATLPHEILEMTNKFMTDPIRILVKRDELT 242

Query: 247 LEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNNFTVSHM 306
           LEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKV+WLTEKMR  NFTVS M
Sbjct: 243 LEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVDWLTEKMREANFTVSSM 302

Query: 307 HGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELYIHRIGR 366
           HGDMPQKER++IM EFRSG +RVLI+TDVWARGLDV QVSL+INYDLPNNRELYIHRIGR
Sbjct: 303 HGDMPQKERESIMKEFRSGASRVLISTDVWARGLDVSQVSLIINYDLPNNRELYIHRIGR 362

Query: 367 SGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI 411
           SGR+GRKGVAINFVK+DDI+ILRDIEQYYSTQIDEMPMNVADLI
Sbjct: 363 SGRYGRKGVAINFVKNDDIRILRDIEQYYSTQIDEMPMNVADLI 406

BLAST of CSPI03G29070 vs. ExPASy TrEMBL
Match: A0A0A0L9P0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G654480 PE=4 SV=1)

HSP 1 Score: 807.7 bits (2085), Expect = 2.2e-230
Identity = 410/410 (100.00%), Postives = 410/410 (100.00%), Query Frame = 0

Query: 1   MAAAATTSVVPPNRRRTISAPEDKLEFETTEGVEPILTFDQMGIKDDLLRGIYAYGFEKP 60
           MAAAATTSVVPPNRRRTISAPEDKLEFETTEGVEPILTFDQMGIKDDLLRGIYAYGFEKP
Sbjct: 1   MAAAATTSVVPPNRRRTISAPEDKLEFETTEGVEPILTFDQMGIKDDLLRGIYAYGFEKP 60

Query: 61  SAIQQRAVRPIIEGRDVIAQAQSGTGKTSMIALTVCQMVDTTSREVQALILSPTRELATQ 120
           SAIQQRAVRPIIEGRDVIAQAQSGTGKTSMIALTVCQMVDTTSREVQALILSPTRELATQ
Sbjct: 61  SAIQQRAVRPIIEGRDVIAQAQSGTGKTSMIALTVCQMVDTTSREVQALILSPTRELATQ 120

Query: 121 TEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAI 180
           TEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAI
Sbjct: 121 TEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAI 180

Query: 181 KLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV 240
           KLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV
Sbjct: 181 KLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV 240

Query: 241 KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN 300
           KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN
Sbjct: 241 KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN 300

Query: 301 FTVSHMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELY 360
           FTVSHMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELY
Sbjct: 301 FTVSHMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELY 360

Query: 361 IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI 411
           IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI
Sbjct: 361 IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI 410

BLAST of CSPI03G29070 vs. ExPASy TrEMBL
Match: A0A5D3BYX9 (Eukaryotic initiation factor 4A-3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1856G00200 PE=4 SV=1)

HSP 1 Score: 801.2 bits (2068), Expect = 2.0e-228
Identity = 409/410 (99.76%), Postives = 409/410 (99.76%), Query Frame = 0

Query: 1   MAAAATTSVVPPNRRRTISAPEDKLEFETTEGVEPILTFDQMGIKDDLLRGIYAYGFEKP 60
           MAAAATTSVVPPNRRR ISAPEDKLEFETTEGVEPILTFDQMGIKDDLLRGIYAYGFEKP
Sbjct: 1   MAAAATTSVVPPNRRR-ISAPEDKLEFETTEGVEPILTFDQMGIKDDLLRGIYAYGFEKP 60

Query: 61  SAIQQRAVRPIIEGRDVIAQAQSGTGKTSMIALTVCQMVDTTSREVQALILSPTRELATQ 120
           SAIQQRAVRPIIEGRDVIAQAQSGTGKTSMIALTVCQMVDTTSREVQALILSPTRELATQ
Sbjct: 61  SAIQQRAVRPIIEGRDVIAQAQSGTGKTSMIALTVCQMVDTTSREVQALILSPTRELATQ 120

Query: 121 TEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAI 180
           TEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAI
Sbjct: 121 TEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAI 180

Query: 181 KLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV 240
           KLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV
Sbjct: 181 KLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV 240

Query: 241 KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN 300
           KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN
Sbjct: 241 KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN 300

Query: 301 FTVSHMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELY 360
           FTVSHMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELY
Sbjct: 301 FTVSHMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELY 360

Query: 361 IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI 411
           IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI
Sbjct: 361 IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI 409

BLAST of CSPI03G29070 vs. ExPASy TrEMBL
Match: A0A1S3CH11 (eukaryotic initiation factor 4A-3 OS=Cucumis melo OX=3656 GN=LOC103500360 PE=4 SV=1)

HSP 1 Score: 801.2 bits (2068), Expect = 2.0e-228
Identity = 409/410 (99.76%), Postives = 409/410 (99.76%), Query Frame = 0

Query: 1   MAAAATTSVVPPNRRRTISAPEDKLEFETTEGVEPILTFDQMGIKDDLLRGIYAYGFEKP 60
           MAAAATTSVVPPNRRR ISAPEDKLEFETTEGVEPILTFDQMGIKDDLLRGIYAYGFEKP
Sbjct: 1   MAAAATTSVVPPNRRR-ISAPEDKLEFETTEGVEPILTFDQMGIKDDLLRGIYAYGFEKP 60

Query: 61  SAIQQRAVRPIIEGRDVIAQAQSGTGKTSMIALTVCQMVDTTSREVQALILSPTRELATQ 120
           SAIQQRAVRPIIEGRDVIAQAQSGTGKTSMIALTVCQMVDTTSREVQALILSPTRELATQ
Sbjct: 61  SAIQQRAVRPIIEGRDVIAQAQSGTGKTSMIALTVCQMVDTTSREVQALILSPTRELATQ 120

Query: 121 TEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAI 180
           TEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAI
Sbjct: 121 TEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAI 180

Query: 181 KLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV 240
           KLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV
Sbjct: 181 KLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV 240

Query: 241 KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN 300
           KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN
Sbjct: 241 KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN 300

Query: 301 FTVSHMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELY 360
           FTVSHMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELY
Sbjct: 301 FTVSHMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELY 360

Query: 361 IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI 411
           IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI
Sbjct: 361 IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI 409

BLAST of CSPI03G29070 vs. ExPASy TrEMBL
Match: A0A6J1K814 (eukaryotic initiation factor 4A-3-like OS=Cucurbita maxima OX=3661 GN=LOC111493076 PE=4 SV=1)

HSP 1 Score: 800.4 bits (2066), Expect = 3.4e-228
Identity = 405/410 (98.78%), Postives = 409/410 (99.76%), Query Frame = 0

Query: 1   MAAAATTSVVPPNRRRTISAPEDKLEFETTEGVEPILTFDQMGIKDDLLRGIYAYGFEKP 60
           MAAAATTSVVPPNRRR I+APEDKLEFETTEGVEPIL+FDQMGIKDDLLRGIYAYGFEKP
Sbjct: 1   MAAAATTSVVPPNRRRAITAPEDKLEFETTEGVEPILSFDQMGIKDDLLRGIYAYGFEKP 60

Query: 61  SAIQQRAVRPIIEGRDVIAQAQSGTGKTSMIALTVCQMVDTTSREVQALILSPTRELATQ 120
           SAIQQRAVRPII+GRDVIAQAQSGTGKTSMIALTVCQMVDT+SREVQALILSPTRELATQ
Sbjct: 61  SAIQQRAVRPIIDGRDVIAQAQSGTGKTSMIALTVCQMVDTSSREVQALILSPTRELATQ 120

Query: 121 TEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAI 180
           TEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAI
Sbjct: 121 TEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAI 180

Query: 181 KLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV 240
           KLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV
Sbjct: 181 KLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV 240

Query: 241 KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN 300
           KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN
Sbjct: 241 KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN 300

Query: 301 FTVSHMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELY 360
           FTVSHMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELY
Sbjct: 301 FTVSHMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELY 360

Query: 361 IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI 411
           IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI
Sbjct: 361 IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI 410

BLAST of CSPI03G29070 vs. ExPASy TrEMBL
Match: A0A6J1GB02 (eukaryotic initiation factor 4A-3-like OS=Cucurbita moschata OX=3662 GN=LOC111452540 PE=4 SV=1)

HSP 1 Score: 800.4 bits (2066), Expect = 3.4e-228
Identity = 405/410 (98.78%), Postives = 409/410 (99.76%), Query Frame = 0

Query: 1   MAAAATTSVVPPNRRRTISAPEDKLEFETTEGVEPILTFDQMGIKDDLLRGIYAYGFEKP 60
           MAAAATTSVVPPNRRR I+APEDKLEFETTEGVEPIL+FDQMGIKDDLLRGIYAYGFEKP
Sbjct: 1   MAAAATTSVVPPNRRRAITAPEDKLEFETTEGVEPILSFDQMGIKDDLLRGIYAYGFEKP 60

Query: 61  SAIQQRAVRPIIEGRDVIAQAQSGTGKTSMIALTVCQMVDTTSREVQALILSPTRELATQ 120
           SAIQQRAVRPII+GRDVIAQAQSGTGKTSMIALTVCQMVDT+SREVQALILSPTRELATQ
Sbjct: 61  SAIQQRAVRPIIDGRDVIAQAQSGTGKTSMIALTVCQMVDTSSREVQALILSPTRELATQ 120

Query: 121 TEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAI 180
           TEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAI
Sbjct: 121 TEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAI 180

Query: 181 KLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV 240
           KLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV
Sbjct: 181 KLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV 240

Query: 241 KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN 300
           KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN
Sbjct: 241 KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN 300

Query: 301 FTVSHMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELY 360
           FTVSHMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELY
Sbjct: 301 FTVSHMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELY 360

Query: 361 IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI 411
           IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI
Sbjct: 361 IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI 410

BLAST of CSPI03G29070 vs. NCBI nr
Match: XP_004148574.1 (eukaryotic initiation factor 4A-3 [Cucumis sativus] >KGN58518.1 hypothetical protein Csa_001953 [Cucumis sativus])

HSP 1 Score: 807.7 bits (2085), Expect = 4.5e-230
Identity = 410/410 (100.00%), Postives = 410/410 (100.00%), Query Frame = 0

Query: 1   MAAAATTSVVPPNRRRTISAPEDKLEFETTEGVEPILTFDQMGIKDDLLRGIYAYGFEKP 60
           MAAAATTSVVPPNRRRTISAPEDKLEFETTEGVEPILTFDQMGIKDDLLRGIYAYGFEKP
Sbjct: 1   MAAAATTSVVPPNRRRTISAPEDKLEFETTEGVEPILTFDQMGIKDDLLRGIYAYGFEKP 60

Query: 61  SAIQQRAVRPIIEGRDVIAQAQSGTGKTSMIALTVCQMVDTTSREVQALILSPTRELATQ 120
           SAIQQRAVRPIIEGRDVIAQAQSGTGKTSMIALTVCQMVDTTSREVQALILSPTRELATQ
Sbjct: 61  SAIQQRAVRPIIEGRDVIAQAQSGTGKTSMIALTVCQMVDTTSREVQALILSPTRELATQ 120

Query: 121 TEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAI 180
           TEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAI
Sbjct: 121 TEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAI 180

Query: 181 KLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV 240
           KLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV
Sbjct: 181 KLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV 240

Query: 241 KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN 300
           KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN
Sbjct: 241 KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN 300

Query: 301 FTVSHMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELY 360
           FTVSHMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELY
Sbjct: 301 FTVSHMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELY 360

Query: 361 IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI 411
           IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI
Sbjct: 361 IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI 410

BLAST of CSPI03G29070 vs. NCBI nr
Match: XP_038905027.1 (eukaryotic initiation factor 4A-3 [Benincasa hispida])

HSP 1 Score: 801.2 bits (2068), Expect = 4.2e-228
Identity = 405/410 (98.78%), Postives = 409/410 (99.76%), Query Frame = 0

Query: 1   MAAAATTSVVPPNRRRTISAPEDKLEFETTEGVEPILTFDQMGIKDDLLRGIYAYGFEKP 60
           MAAAATTSVVPPNRRRT++APEDKLEFETTEGVEPILTFDQMGIKDDLLRGIYAYGFEKP
Sbjct: 1   MAAAATTSVVPPNRRRTVNAPEDKLEFETTEGVEPILTFDQMGIKDDLLRGIYAYGFEKP 60

Query: 61  SAIQQRAVRPIIEGRDVIAQAQSGTGKTSMIALTVCQMVDTTSREVQALILSPTRELATQ 120
           SAIQQRAVRPIIEGRDVIAQAQSGTGKTSMIALTVCQMVDT+SREVQALILSPTRELATQ
Sbjct: 61  SAIQQRAVRPIIEGRDVIAQAQSGTGKTSMIALTVCQMVDTSSREVQALILSPTRELATQ 120

Query: 121 TEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAI 180
           T KVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAI
Sbjct: 121 TAKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAI 180

Query: 181 KLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV 240
           KLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV
Sbjct: 181 KLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV 240

Query: 241 KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN 300
           KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN
Sbjct: 241 KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN 300

Query: 301 FTVSHMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELY 360
           FTVSHMHGDMPQ+ERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELY
Sbjct: 301 FTVSHMHGDMPQRERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELY 360

Query: 361 IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI 411
           IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI
Sbjct: 361 IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI 410

BLAST of CSPI03G29070 vs. NCBI nr
Match: XP_008461865.1 (PREDICTED: eukaryotic initiation factor 4A-3 [Cucumis melo] >TYK04194.1 eukaryotic initiation factor 4A-3 [Cucumis melo var. makuwa])

HSP 1 Score: 801.2 bits (2068), Expect = 4.2e-228
Identity = 409/410 (99.76%), Postives = 409/410 (99.76%), Query Frame = 0

Query: 1   MAAAATTSVVPPNRRRTISAPEDKLEFETTEGVEPILTFDQMGIKDDLLRGIYAYGFEKP 60
           MAAAATTSVVPPNRRR ISAPEDKLEFETTEGVEPILTFDQMGIKDDLLRGIYAYGFEKP
Sbjct: 1   MAAAATTSVVPPNRRR-ISAPEDKLEFETTEGVEPILTFDQMGIKDDLLRGIYAYGFEKP 60

Query: 61  SAIQQRAVRPIIEGRDVIAQAQSGTGKTSMIALTVCQMVDTTSREVQALILSPTRELATQ 120
           SAIQQRAVRPIIEGRDVIAQAQSGTGKTSMIALTVCQMVDTTSREVQALILSPTRELATQ
Sbjct: 61  SAIQQRAVRPIIEGRDVIAQAQSGTGKTSMIALTVCQMVDTTSREVQALILSPTRELATQ 120

Query: 121 TEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAI 180
           TEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAI
Sbjct: 121 TEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAI 180

Query: 181 KLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV 240
           KLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV
Sbjct: 181 KLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV 240

Query: 241 KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN 300
           KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN
Sbjct: 241 KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN 300

Query: 301 FTVSHMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELY 360
           FTVSHMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELY
Sbjct: 301 FTVSHMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELY 360

Query: 361 IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI 411
           IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI
Sbjct: 361 IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI 409

BLAST of CSPI03G29070 vs. NCBI nr
Match: XP_022949081.1 (eukaryotic initiation factor 4A-3-like [Cucurbita moschata] >XP_022998447.1 eukaryotic initiation factor 4A-3-like [Cucurbita maxima] >XP_023525647.1 eukaryotic initiation factor 4A-3-like [Cucurbita pepo subsp. pepo] >KAG6606907.1 hypothetical protein SDJN03_00249, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 800.4 bits (2066), Expect = 7.1e-228
Identity = 405/410 (98.78%), Postives = 409/410 (99.76%), Query Frame = 0

Query: 1   MAAAATTSVVPPNRRRTISAPEDKLEFETTEGVEPILTFDQMGIKDDLLRGIYAYGFEKP 60
           MAAAATTSVVPPNRRR I+APEDKLEFETTEGVEPIL+FDQMGIKDDLLRGIYAYGFEKP
Sbjct: 1   MAAAATTSVVPPNRRRAITAPEDKLEFETTEGVEPILSFDQMGIKDDLLRGIYAYGFEKP 60

Query: 61  SAIQQRAVRPIIEGRDVIAQAQSGTGKTSMIALTVCQMVDTTSREVQALILSPTRELATQ 120
           SAIQQRAVRPII+GRDVIAQAQSGTGKTSMIALTVCQMVDT+SREVQALILSPTRELATQ
Sbjct: 61  SAIQQRAVRPIIDGRDVIAQAQSGTGKTSMIALTVCQMVDTSSREVQALILSPTRELATQ 120

Query: 121 TEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAI 180
           TEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAI
Sbjct: 121 TEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAI 180

Query: 181 KLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV 240
           KLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV
Sbjct: 181 KLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV 240

Query: 241 KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN 300
           KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN
Sbjct: 241 KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN 300

Query: 301 FTVSHMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELY 360
           FTVSHMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELY
Sbjct: 301 FTVSHMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELY 360

Query: 361 IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI 411
           IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI
Sbjct: 361 IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI 410

BLAST of CSPI03G29070 vs. NCBI nr
Match: XP_022983495.1 (eukaryotic initiation factor 4A-3 [Cucurbita maxima] >KAG7018051.1 hypothetical protein SDJN02_19917, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 799.3 bits (2063), Expect = 1.6e-227
Identity = 403/410 (98.29%), Postives = 409/410 (99.76%), Query Frame = 0

Query: 1   MAAAATTSVVPPNRRRTISAPEDKLEFETTEGVEPILTFDQMGIKDDLLRGIYAYGFEKP 60
           MAAAATTSVVPPNRRR +SAPEDKLEFETTEGVEPIL+FDQMGIKDDLLRGIYAYGFEKP
Sbjct: 1   MAAAATTSVVPPNRRRAVSAPEDKLEFETTEGVEPILSFDQMGIKDDLLRGIYAYGFEKP 60

Query: 61  SAIQQRAVRPIIEGRDVIAQAQSGTGKTSMIALTVCQMVDTTSREVQALILSPTRELATQ 120
           SAIQQRAVRPIIEGRDVIAQAQSGTGKTSMIALTVCQ+VDT+SREVQALILSPTRELATQ
Sbjct: 61  SAIQQRAVRPIIEGRDVIAQAQSGTGKTSMIALTVCQVVDTSSREVQALILSPTRELATQ 120

Query: 121 TEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAI 180
           TEKVILAIGDYINIQAH+CIGGKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAI
Sbjct: 121 TEKVILAIGDYINIQAHSCIGGKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAI 180

Query: 181 KLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV 240
           KLL+LDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV
Sbjct: 181 KLLILDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV 240

Query: 241 KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN 300
           KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN
Sbjct: 241 KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN 300

Query: 301 FTVSHMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELY 360
           FTVSHMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELY
Sbjct: 301 FTVSHMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELY 360

Query: 361 IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI 411
           IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI
Sbjct: 361 IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI 410

BLAST of CSPI03G29070 vs. TAIR 10
Match: AT3G19760.1 (eukaryotic initiation factor 4A-III )

HSP 1 Score: 695.7 bits (1794), Expect = 2.3e-200
Identity = 347/397 (87.41%), Postives = 376/397 (94.71%), Query Frame = 0

Query: 14  RRRTISAPEDKLEFETTEGVEPILTFDQMGIKDDLLRGIYAYGFEKPSAIQQRAVRPIIE 73
           RR   +  +DKL FETT+G+EPI +F+ MGIK+D+LRG+Y YGFEKPSAIQQRAV PI++
Sbjct: 12  RRGGGAMDDDKLVFETTDGIEPITSFNDMGIKEDVLRGVYEYGFEKPSAIQQRAVMPILQ 71

Query: 74  GRDVIAQAQSGTGKTSMIALTVCQMVDTTSREVQALILSPTRELATQTEKVILAIGDYIN 133
           GRDVIAQAQSGTGKTSMIAL+VCQ+VDT+SREVQALILSPTRELATQTEK I AIG + N
Sbjct: 72  GRDVIAQAQSGTGKTSMIALSVCQVVDTSSREVQALILSPTRELATQTEKTIQAIGLHAN 131

Query: 134 IQAHACIGGKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAIKLLVLDESDEMLS 193
           IQAHACIGG SVGEDIRKLE GV VVSGTPGRVCDMIKRR+LRTRAIKLL+LDESDEMLS
Sbjct: 132 IQAHACIGGNSVGEDIRKLEHGVHVVSGTPGRVCDMIKRRSLRTRAIKLLILDESDEMLS 191

Query: 194 RGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILVKRDELTLEGIKQF 253
           RGFKDQIYDVYRYLPP+LQV L+SATLPHEILEMT+KFMT+PV+ILVKRDELTLEGIKQF
Sbjct: 192 RGFKDQIYDVYRYLPPDLQVCLVSATLPHEILEMTSKFMTEPVKILVKRDELTLEGIKQF 251

Query: 254 FVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNNFTVSHMHGDMPQK 313
           FVAVE+EEWKFDTLCDLYDTLTITQAVIFCNTKRKV++L+EKMRS+NFTVS MHGDMPQK
Sbjct: 252 FVAVEKEEWKFDTLCDLYDTLTITQAVIFCNTKRKVDYLSEKMRSHNFTVSSMHGDMPQK 311

Query: 314 ERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELYIHRIGRSGRFGRK 373
           ERDAIM EFRSG +RVLITTDVWARG+DVQQVSLVINYDLPNNRELYIHRIGRSGRFGRK
Sbjct: 312 ERDAIMNEFRSGDSRVLITTDVWARGIDVQQVSLVINYDLPNNRELYIHRIGRSGRFGRK 371

Query: 374 GVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI 411
           GVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI
Sbjct: 372 GVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI 408

BLAST of CSPI03G29070 vs. TAIR 10
Match: AT1G51380.1 (DEA(D/H)-box RNA helicase family protein )

HSP 1 Score: 575.9 bits (1483), Expect = 2.7e-164
Identity = 285/389 (73.26%), Postives = 339/389 (87.15%), Query Frame = 0

Query: 22  EDKLEFETTEGVEPILTFDQMGIKDDLLRGIYAYGFEKPSAIQQRAVRPIIEGRDVIAQA 81
           E+ L FETT+G++PI +FD MG+ D +LRG+Y YG++KPS IQQRA+ PI++GRDVIAQA
Sbjct: 7   EENLVFETTKGIKPIKSFDDMGMNDKVLRGVYDYGYKKPSEIQQRALVPILKGRDVIAQA 66

Query: 82  QSGTGKTSMIALTVCQMVDTTSREVQALILSPTRELATQTEKVILAIGDYINIQAHACIG 141
           QSGTGKTSMIA++VCQ+V+ +SR+VQ L+LSP+RELA+QTEK I AIG + NIQAHACIG
Sbjct: 67  QSGTGKTSMIAISVCQIVNISSRKVQVLVLSPSRELASQTEKTIQAIGAHTNIQAHACIG 126

Query: 142 GKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAIKLLVLDESDEMLSRGFKDQIY 201
           GKS+GEDI+KLE GV  VSGTPGRV DMIKR +L+T+A+KLLVLDESDEMLS+G KDQIY
Sbjct: 127 GKSIGEDIKKLERGVHAVSGTPGRVYDMIKRGSLQTKAVKLLVLDESDEMLSKGLKDQIY 186

Query: 202 DVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILVKRDELTLEGIKQFFVAVEREE 261
           DVYR LP ++QV LISATLP EILEMT KFMTDPVRILVK DELTLEGIKQ++V V++EE
Sbjct: 187 DVYRALPHDIQVCLISATLPQEILEMTEKFMTDPVRILVKPDELTLEGIKQYYVDVDKEE 246

Query: 262 WKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNNFTVSHMHGDMPQKERDAIMGE 321
           WKFDTLCDLY  LTI QA+IFCNT++KV+WLTEKMRS+NF VS MHGD  QKERD IM +
Sbjct: 247 WKFDTLCDLYGRLTINQAIIFCNTRQKVDWLTEKMRSSNFIVSSMHGDKRQKERDDIMNQ 306

Query: 322 FRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELYIHRIGRSGRFGRKGVAINFVK 381
           FRS  +RVLI +DVWARG+DVQ VS VINYD+PNN ELYIHRIGR+GRFGR+GVAINFVK
Sbjct: 307 FRSFKSRVLIASDVWARGIDVQTVSHVINYDIPNNPELYIHRIGRAGRFGREGVAINFVK 366

Query: 382 SDDIKILRDIEQYYSTQIDEMPMNVADLI 411
           S D+K L+DIE++Y T+I EMP   ADL+
Sbjct: 367 SSDMKDLKDIERHYGTKIREMP---ADLV 392

BLAST of CSPI03G29070 vs. TAIR 10
Match: AT3G13920.1 (eukaryotic translation initiation factor 4A1 )

HSP 1 Score: 502.3 bits (1292), Expect = 3.7e-142
Identity = 247/373 (66.22%), Postives = 306/373 (82.04%), Query Frame = 0

Query: 38  TFDQMGIKDDLLRGIYAYGFEKPSAIQQRAVRPIIEGRDVIAQAQSGTGKTSMIALTVCQ 97
           +FD MG++++LLRGIYAYGFEKPSAIQQR + P  +G DVI QAQSGTGKT+     V Q
Sbjct: 40  SFDAMGLQENLLRGIYAYGFEKPSAIQQRGIVPFCKGLDVIQQAQSGTGKTATFCSGVLQ 99

Query: 98  MVDTTSREVQALILSPTRELATQTEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQ 157
            +D +  + QAL+L+PTRELA Q EKV+ A+GDY+ ++ HAC+GG SV ED R L+ GV 
Sbjct: 100 QLDFSLIQCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVREDQRILQAGVH 159

Query: 158 VVSGTPGRVCDMIKRRTLRTRAIKLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLIS 217
           VV GTPGRV DM+KR++LR   IK+ VLDE+DEMLSRGFKDQIYD+++ LPP++QV + S
Sbjct: 160 VVVGTPGRVFDMLKRQSLRADNIKMFVLDEADEMLSRGFKDQIYDIFQLLPPKIQVGVFS 219

Query: 218 ATLPHEILEMTNKFMTDPVRILVKRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTIT 277
           AT+P E LE+T KFM+ PVRILVKRDELTLEGIKQF+V VE+EEWK +TLCDLY+TL IT
Sbjct: 220 ATMPPEALEITRKFMSKPVRILVKRDELTLEGIKQFYVNVEKEEWKLETLCDLYETLAIT 279

Query: 278 QAVIFCNTKRKVEWLTEKMRSNNFTVSHMHGDMPQKERDAIMGEFRSGTTRVLITTDVWA 337
           Q+VIF NT+RKV+WLT+KMRS + TVS  HGDM Q  RD IM EFRSG++RVLITTD+ A
Sbjct: 280 QSVIFVNTRRKVDWLTDKMRSRDHTVSATHGDMDQNTRDIIMREFRSGSSRVLITTDLLA 339

Query: 338 RGLDVQQVSLVINYDLPNNRELYIHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYST 397
           RG+DVQQVSLVIN+DLP   E Y+HRIGRSGRFGRKGVAINFV  DD ++L DI+++Y+ 
Sbjct: 340 RGIDVQQVSLVINFDLPTQPENYLHRIGRSGRFGRKGVAINFVTRDDERMLFDIQKFYNV 399

Query: 398 QIDEMPMNVADLI 411
            ++E+P NVADL+
Sbjct: 400 VVEELPSNVADLL 412

BLAST of CSPI03G29070 vs. TAIR 10
Match: AT3G13920.3 (eukaryotic translation initiation factor 4A1 )

HSP 1 Score: 502.3 bits (1292), Expect = 3.7e-142
Identity = 247/373 (66.22%), Postives = 306/373 (82.04%), Query Frame = 0

Query: 38  TFDQMGIKDDLLRGIYAYGFEKPSAIQQRAVRPIIEGRDVIAQAQSGTGKTSMIALTVCQ 97
           +FD MG++++LLRGIYAYGFEKPSAIQQR + P  +G DVI QAQSGTGKT+     V Q
Sbjct: 30  SFDAMGLQENLLRGIYAYGFEKPSAIQQRGIVPFCKGLDVIQQAQSGTGKTATFCSGVLQ 89

Query: 98  MVDTTSREVQALILSPTRELATQTEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQ 157
            +D +  + QAL+L+PTRELA Q EKV+ A+GDY+ ++ HAC+GG SV ED R L+ GV 
Sbjct: 90  QLDFSLIQCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVREDQRILQAGVH 149

Query: 158 VVSGTPGRVCDMIKRRTLRTRAIKLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLIS 217
           VV GTPGRV DM+KR++LR   IK+ VLDE+DEMLSRGFKDQIYD+++ LPP++QV + S
Sbjct: 150 VVVGTPGRVFDMLKRQSLRADNIKMFVLDEADEMLSRGFKDQIYDIFQLLPPKIQVGVFS 209

Query: 218 ATLPHEILEMTNKFMTDPVRILVKRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTIT 277
           AT+P E LE+T KFM+ PVRILVKRDELTLEGIKQF+V VE+EEWK +TLCDLY+TL IT
Sbjct: 210 ATMPPEALEITRKFMSKPVRILVKRDELTLEGIKQFYVNVEKEEWKLETLCDLYETLAIT 269

Query: 278 QAVIFCNTKRKVEWLTEKMRSNNFTVSHMHGDMPQKERDAIMGEFRSGTTRVLITTDVWA 337
           Q+VIF NT+RKV+WLT+KMRS + TVS  HGDM Q  RD IM EFRSG++RVLITTD+ A
Sbjct: 270 QSVIFVNTRRKVDWLTDKMRSRDHTVSATHGDMDQNTRDIIMREFRSGSSRVLITTDLLA 329

Query: 338 RGLDVQQVSLVINYDLPNNRELYIHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYST 397
           RG+DVQQVSLVIN+DLP   E Y+HRIGRSGRFGRKGVAINFV  DD ++L DI+++Y+ 
Sbjct: 330 RGIDVQQVSLVINFDLPTQPENYLHRIGRSGRFGRKGVAINFVTRDDERMLFDIQKFYNV 389

Query: 398 QIDEMPMNVADLI 411
            ++E+P NVADL+
Sbjct: 390 VVEELPSNVADLL 402

BLAST of CSPI03G29070 vs. TAIR 10
Match: AT1G54270.1 (eif4a-2 )

HSP 1 Score: 498.0 bits (1281), Expect = 7.0e-141
Identity = 245/373 (65.68%), Postives = 304/373 (81.50%), Query Frame = 0

Query: 38  TFDQMGIKDDLLRGIYAYGFEKPSAIQQRAVRPIIEGRDVIAQAQSGTGKTSMIALTVCQ 97
           +FD MG++++LLRGIYAYGFEKPSAIQQR + P  +G DVI QAQSGTGKT+     V Q
Sbjct: 40  SFDAMGLQENLLRGIYAYGFEKPSAIQQRGIVPFCKGLDVIQQAQSGTGKTATFCSGVLQ 99

Query: 98  MVDTTSREVQALILSPTRELATQTEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQ 157
            +D    + QAL+L+PTRELA Q EKV+ A+GDY  ++ HAC+GG SV ED R L+ GV 
Sbjct: 100 QLDYALLQCQALVLAPTRELAQQIEKVMRALGDYQGVKVHACVGGTSVREDQRILQAGVH 159

Query: 158 VVSGTPGRVCDMIKRRTLRTRAIKLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLIS 217
           VV GTPGRV DM++R++LR   IK+ VLDE+DEMLSRGFKDQIYD+++ LPP++QV + S
Sbjct: 160 VVVGTPGRVFDMLRRQSLRPDCIKMFVLDEADEMLSRGFKDQIYDIFQLLPPKIQVGVFS 219

Query: 218 ATLPHEILEMTNKFMTDPVRILVKRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTIT 277
           AT+P E LE+T KFM+ PVRILVKRDELTLEGIKQF+V VE+E+WK +TLCDLY+TL IT
Sbjct: 220 ATMPPEALEITRKFMSKPVRILVKRDELTLEGIKQFYVNVEKEDWKLETLCDLYETLAIT 279

Query: 278 QAVIFCNTKRKVEWLTEKMRSNNFTVSHMHGDMPQKERDAIMGEFRSGTTRVLITTDVWA 337
           Q+VIF NT+RKV+WLT+KMRS + TVS  HGDM Q  RD IM EFRSG++RVLITTD+ A
Sbjct: 280 QSVIFVNTRRKVDWLTDKMRSRDHTVSATHGDMDQNTRDIIMREFRSGSSRVLITTDLLA 339

Query: 338 RGLDVQQVSLVINYDLPNNRELYIHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYST 397
           RG+DVQQVSLVIN+DLP   E Y+HRIGRSGRFGRKGVAINFV  DD ++L DI+++Y+ 
Sbjct: 340 RGIDVQQVSLVINFDLPTQPENYLHRIGRSGRFGRKGVAINFVTLDDQRMLFDIQKFYNV 399

Query: 398 QIDEMPMNVADLI 411
            ++E+P NVADL+
Sbjct: 400 VVEELPSNVADLL 412

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P413802.3e-20592.29Eukaryotic initiation factor 4A-3 OS=Nicotiana plumbaginifolia OX=4092 PE=2 SV=1[more]
Q5VNM34.8e-20387.99Eukaryotic initiation factor 4A-III homolog A OS=Oryza sativa subsp. japonica OX... [more]
Q10I264.8e-20387.99Eukaryotic initiation factor 4A-III homolog B OS=Oryza sativa subsp. japonica OX... [more]
Q94A523.2e-19987.41Eukaryotic initiation factor 4A-III homolog OS=Arabidopsis thaliana OX=3702 GN=E... [more]
Q7ZVA62.7e-18579.21Eukaryotic initiation factor 4A-III OS=Danio rerio OX=7955 GN=eif4a3 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0L9P02.2e-230100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G654480 PE=4 SV=1[more]
A0A5D3BYX92.0e-22899.76Eukaryotic initiation factor 4A-3 OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
A0A1S3CH112.0e-22899.76eukaryotic initiation factor 4A-3 OS=Cucumis melo OX=3656 GN=LOC103500360 PE=4 S... [more]
A0A6J1K8143.4e-22898.78eukaryotic initiation factor 4A-3-like OS=Cucurbita maxima OX=3661 GN=LOC1114930... [more]
A0A6J1GB023.4e-22898.78eukaryotic initiation factor 4A-3-like OS=Cucurbita moschata OX=3662 GN=LOC11145... [more]
Match NameE-valueIdentityDescription
XP_004148574.14.5e-230100.00eukaryotic initiation factor 4A-3 [Cucumis sativus] >KGN58518.1 hypothetical pro... [more]
XP_038905027.14.2e-22898.78eukaryotic initiation factor 4A-3 [Benincasa hispida][more]
XP_008461865.14.2e-22899.76PREDICTED: eukaryotic initiation factor 4A-3 [Cucumis melo] >TYK04194.1 eukaryot... [more]
XP_022949081.17.1e-22898.78eukaryotic initiation factor 4A-3-like [Cucurbita moschata] >XP_022998447.1 euka... [more]
XP_022983495.11.6e-22798.29eukaryotic initiation factor 4A-3 [Cucurbita maxima] >KAG7018051.1 hypothetical ... [more]
Match NameE-valueIdentityDescription
AT3G19760.12.3e-20087.41eukaryotic initiation factor 4A-III [more]
AT1G51380.12.7e-16473.26DEA(D/H)-box RNA helicase family protein [more]
AT3G13920.13.7e-14266.22eukaryotic translation initiation factor 4A1 [more]
AT3G13920.33.7e-14266.22eukaryotic translation initiation factor 4A1 [more]
AT1G54270.17.0e-14165.68eif4a-2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001650Helicase, C-terminalSMARTSM00490helicmild6coord: 290..371
e-value: 7.6E-34
score: 128.4
IPR001650Helicase, C-terminalPFAMPF00271Helicase_Ccoord: 263..371
e-value: 3.1E-31
score: 108.0
IPR001650Helicase, C-terminalPROSITEPS51194HELICASE_CTERcoord: 249..410
score: 26.851318
IPR014001Helicase superfamily 1/2, ATP-binding domainSMARTSM00487ultradead3coord: 56..253
e-value: 8.1E-52
score: 188.1
IPR014001Helicase superfamily 1/2, ATP-binding domainPROSITEPS51192HELICASE_ATP_BIND_1coord: 68..238
score: 25.253036
IPR011545DEAD/DEAH box helicase domainPFAMPF00270DEADcoord: 62..224
e-value: 3.7E-41
score: 140.8
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 22..241
e-value: 9.6E-64
score: 216.5
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 242..410
e-value: 4.2E-60
score: 203.8
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 105..393
NoneNo IPR availablePANTHERPTHR47958ATP-DEPENDENT RNA HELICASE DBP3coord: 22..403
NoneNo IPR availablePANTHERPTHR47958:SF55BNAC03G41130D PROTEINcoord: 22..403
NoneNo IPR availableCDDcd18045DEADc_EIF4AIII_DDX48coord: 39..239
e-value: 2.24719E-122
score: 350.999
NoneNo IPR availableCDDcd18787SF2_C_DEADcoord: 250..380
e-value: 1.65034E-62
score: 195.804
IPR014014RNA helicase, DEAD-box type, Q motifPROSITEPS51195Q_MOTIFcoord: 37..65
score: 10.728894

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI03G29070.1CSPI03G29070.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006413 translational initiation
cellular_component GO:0071013 catalytic step 2 spliceosome
cellular_component GO:0005730 nucleolus
molecular_function GO:0005524 ATP binding
molecular_function GO:0016787 hydrolase activity
molecular_function GO:0003729 mRNA binding
molecular_function GO:0003724 RNA helicase activity
molecular_function GO:0003743 translation initiation factor activity
molecular_function GO:0003676 nucleic acid binding