Homology
BLAST of CSPI03G28630 vs. ExPASy Swiss-Prot
Match:
O04886 (Pectinesterase 1 OS=Citrus sinensis OX=2711 GN=PECS-1.1 PE=2 SV=1)
HSP 1 Score: 713.0 bits (1839), Expect = 2.7e-204
Identity = 359/523 (68.64%), Postives = 428/523 (81.84%), Query Frame = 0
Query: 44 SKCAMTLYPELCETTISTAVG-----SSSKEAIEASVNITISAVKDNYKRVQKLLKTVKN 103
S C+ T YP+LC + I+ +S K+ IE S+NIT +AV+ NY +QKLLK N
Sbjct: 71 SSCSSTRYPDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKLLKRT-N 130
Query: 104 LTKRQKIAFHDCLETGEETLRELYEVVEDVNEYPKKKSLSRYADDLKTLLSSAITNQETC 163
LTKR+K+A HDCLET +ETL EL++ VED+ EYP KKSLS++ADDLKTL+S+A+TNQ TC
Sbjct: 131 LTKREKVALHDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTNQGTC 190
Query: 164 VDGFSHDKGDKKVRESLKEGLIHIEKLCSIALALIKNLTDTDIANLNNNNNNNHLNRKQL 223
+DGFSHD +K VR++L +G +H+EK+CS ALA+IKN+TDTD+ + +N NRK
Sbjct: 191 LDGFSHDDANKHVRDALSDGQVHVEKMCSNALAMIKNMTDTDMMIMRTSN-----NRKLT 250
Query: 224 EEKKMEDGIKWPDWMSPKDRRLLQASSTATPDVVVAADGSGDFRTISEAVAAAPSRSSRR 283
EE DG WP W+SP DRRLLQ SS+ TP+ VVAADGSG+F+T++ AVAAAP ++R
Sbjct: 251 EETSTVDG--WPAWLSPGDRRLLQ-SSSVTPNAVVAADGSGNFKTVAAAVAAAPQGGTKR 310
Query: 284 YIIRIKAGVYRENVNVASSKRNIMFWGDGRVNTIITGNRNVVDGSTTFNSATVAAVGERF 343
YIIRIKAGVYRENV V +NIMF GDGR TIITG+RNVVDGSTTF SAT A VGE F
Sbjct: 311 YIIRIKAGVYRENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATAAVVGEGF 370
Query: 344 LARDVTFQNTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVQCIIVGTI 403
LARD+TFQNTAGPSKHQAVALRVG+DLSAFY CDMLAYQDTLYVHSNRQF+V C+I GT+
Sbjct: 371 LARDITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNCLIAGTV 430
Query: 404 DFIFGNAAAVIQDCDIHARRPNPGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVI 463
DFIFGNAAAV+Q+CDIHAR+PN GQ+NMVTAQGRTDPNQNTGIVIQK RIG TSDL+PV
Sbjct: 431 DFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRTDPNQNTGIVIQKSRIGATSDLKPVQ 490
Query: 464 SNFPTFLGRPWQRYSRTVVMQTSISNVIDPAGWHVWDGNFALDTLFYAEYQNSGAGADTS 523
+FPT+LGRPW+ YSRTV+MQ+SI+++I PAGWH WDGNFAL+TLFY E+QNSGAGA TS
Sbjct: 491 GSFPTYLGRPWKEYSRTVIMQSSITDLIHPAGWHEWDGNFALNTLFYGEHQNSGAGAGTS 550
Query: 524 RRVKWKGFRVLTRAAEAEAFTAGNFIGGGTWLSSTGFPFSLGL 562
RVKWKGFRV+T A EA+AFT G+FI G +WL STGFPFSLGL
Sbjct: 551 GRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584
BLAST of CSPI03G28630 vs. ExPASy Swiss-Prot
Match:
P83948 (Pectinesterase 3 OS=Citrus sinensis OX=2711 PE=1 SV=1)
HSP 1 Score: 711.1 bits (1834), Expect = 1.0e-203
Identity = 358/523 (68.45%), Postives = 429/523 (82.03%), Query Frame = 0
Query: 44 SKCAMTLYPELCETTISTAVG-----SSSKEAIEASVNITISAVKDNYKRVQKLLKTVKN 103
S C+ T YP+LC + I+ +S K+ IE S+NIT +AV+ NY +QKLLK N
Sbjct: 71 SSCSSTRYPDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKLLKRT-N 130
Query: 104 LTKRQKIAFHDCLETGEETLRELYEVVEDVNEYPKKKSLSRYADDLKTLLSSAITNQETC 163
LTKR+K+A HDCLET +ETL EL++ VED+ EYP KKSLS++ADDLKTL+S+A+TNQ TC
Sbjct: 131 LTKREKVALHDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTNQGTC 190
Query: 164 VDGFSHDKGDKKVRESLKEGLIHIEKLCSIALALIKNLTDTDIANLNNNNNNNHLNRKQL 223
+DGFSHD +K VR++L +G +H+EK+CS ALA+IKN+TDTD+ + +N NRK +
Sbjct: 191 LDGFSHDDANKHVRDALSDGQVHVEKMCSNALAMIKNMTDTDMMIMRTSN-----NRKLI 250
Query: 224 EEKKMEDGIKWPDWMSPKDRRLLQASSTATPDVVVAADGSGDFRTISEAVAAAPSRSSRR 283
EE DG WP W+S DRRLLQ SS+ TP+VVVAADGSG+F+T++ +VAAAP ++R
Sbjct: 251 EETSTVDG--WPAWLSTGDRRLLQ-SSSVTPNVVVAADGSGNFKTVAASVAAAPQGGTKR 310
Query: 284 YIIRIKAGVYRENVNVASSKRNIMFWGDGRVNTIITGNRNVVDGSTTFNSATVAAVGERF 343
YIIRIKAGVYRENV V +NIMF GDGR TIITG+RNVVDGSTTF SATVA VGE F
Sbjct: 311 YIIRIKAGVYRENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATVAVVGEGF 370
Query: 344 LARDVTFQNTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVQCIIVGTI 403
LARD+TFQNTAGPSKHQAVALRVG+DLSAFY CDMLAYQDTLYVHSNRQF+V C+I GT+
Sbjct: 371 LARDITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNCLIAGTV 430
Query: 404 DFIFGNAAAVIQDCDIHARRPNPGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVI 463
DFIFGNAAAV+Q+CDIHAR+PN GQ+NMVTAQGR DPNQNTGIVIQK RIG TSDL+PV
Sbjct: 431 DFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRADPNQNTGIVIQKSRIGATSDLKPVQ 490
Query: 464 SNFPTFLGRPWQRYSRTVVMQTSISNVIDPAGWHVWDGNFALDTLFYAEYQNSGAGADTS 523
+FPT+LGRPW+ YSRTV+MQ+SI++VI PAGWH WDGNFAL+TLFY E+QN+GAGA TS
Sbjct: 491 GSFPTYLGRPWKEYSRTVIMQSSITDVIHPAGWHEWDGNFALNTLFYGEHQNAGAGAGTS 550
Query: 524 RRVKWKGFRVLTRAAEAEAFTAGNFIGGGTWLSSTGFPFSLGL 562
RVKWKGFRV+T A EA+AFT G+FI G +WL STGFPFSLGL
Sbjct: 551 GRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584
BLAST of CSPI03G28630 vs. ExPASy Swiss-Prot
Match:
Q43143 (Pectinesterase/pectinesterase inhibitor U1 OS=Solanum lycopersicum OX=4081 GN=PMEU1 PE=2 SV=1)
HSP 1 Score: 710.3 bits (1832), Expect = 1.7e-203
Identity = 370/590 (62.71%), Postives = 442/590 (74.92%), Query Frame = 0
Query: 1 MSKPDDTPAAGVPFSGYSKTNLLVFALSTTLLLAVV-----------SSDDH-------- 60
M++ +D + + F K L S L+ AV+ +SDDH
Sbjct: 1 MTRVEDFFSKQIDFCKRKKKIYLAIVASVLLVAAVIGVVAGVKSHSKNSDDHADIMAISS 60
Query: 61 -----VGSKCAMTLYPELCETTISTAVG-----SSSKEAIEASVNITISAVKDNYKRVQK 120
V S C+ TL+PELC + I +S K+ IE S+NIT+ AV+ NY V++
Sbjct: 61 SAHAIVKSACSNTLHPELCYSAIVNVSDFSKKVTSQKDVIELSLNITVKAVRRNYYAVKE 120
Query: 121 LLKTVKNLTKRQKIAFHDCLETGEETLRELYEVVEDVNEYPKKKSLSRYADDLKTLLSSA 180
L+KT K LT R+K+A HDCLET +ETL EL+ VED+ YP KKSL + +DLKTL+SSA
Sbjct: 121 LIKTRKGLTPREKVALHDCLETMDETLDELHTAVEDLELYPNKKSLKEHVEDLKTLISSA 180
Query: 181 ITNQETCVDGFSHDKGDKKVRESLKEGLIHIEKLCSIALALIKNLTDTDIANLNNNNNNN 240
ITNQETC+DGFSHD+ DKKVR+ L +G H+EK+CS ALA+I N+TDTDIA N
Sbjct: 181 ITNQETCLDGFSHDEADKKVRKVLLKGQKHVEKMCSNALAMICNMTDTDIA------NEM 240
Query: 241 HLNRKQLEEKKMEDGIKWPDWMSPKDRRLLQASSTATPDVVVAADGSGDFRTISEAVAAA 300
L+ K +ED +WP+W+S DRRLLQ SST TPDVVVAADGSGD++T+SEAV A
Sbjct: 241 KLSAPANNRKLVEDNGEWPEWLSAGDRRLLQ-SSTVTPDVVVAADGSGDYKTVSEAVRKA 300
Query: 301 PSRSSRRYIIRIKAGVYRENVNVASSKRNIMFWGDGRVNTIITGNRNVVDGSTTFNSATV 360
P +SS+RY+IRIKAGVYRENV+V K NIMF GDG+ NTIIT +RNV DGSTTF+SATV
Sbjct: 301 PEKSSKRYVIRIKAGVYRENVDVPKKKTNIMFMGDGKSNTIITASRNVQDGSTTFHSATV 360
Query: 361 AAVGERFLARDVTFQNTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVQ 420
V + LARD+TFQNTAG SKHQAVAL VGSDLSAFYRCDMLAYQDTLYVHSNRQF+VQ
Sbjct: 361 VRVAGKVLARDITFQNTAGASKHQAVALCVGSDLSAFYRCDMLAYQDTLYVHSNRQFFVQ 420
Query: 421 CIIVGTIDFIFGNAAAVIQDCDIHARRPNPGQRNMVTAQGRTDPNQNTGIVIQKCRIGTT 480
C++ GT+DFIFGN AAV QDCDIHARRP GQ+NMVTAQGRTDPNQNTGIVIQKCRIG T
Sbjct: 421 CLVAGTVDFIFGNGAAVFQDCDIHARRPGSGQKNMVTAQGRTDPNQNTGIVIQKCRIGAT 480
Query: 481 SDLRPVISNFPTFLGRPWQRYSRTVVMQTSISNVIDPAGWHVWDGNFALDTLFYAEYQNS 540
SDLRPV +FPT+LGRPW+ YSRTV+MQ+SI++VI PAGWH W+GNFALDTLFY EY N+
Sbjct: 481 SDLRPVQKSFPTYLGRPWKEYSRTVIMQSSITDVIQPAGWHEWNGNFALDTLFYGEYANT 540
Query: 541 GAGADTSRRVKWKGFRVLTRAAEAEAFTAGNFIGGGTWLSSTGFPFSLGL 562
GAGA TS RVKWKG +V+T + EA+A+T G FI GG+WLSSTGFPFSLGL
Sbjct: 541 GAGAPTSGRVKWKGHKVITSSTEAQAYTPGRFIAGGSWLSSTGFPFSLGL 583
BLAST of CSPI03G28630 vs. ExPASy Swiss-Prot
Match:
O49006 (Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PME3 PE=1 SV=2)
HSP 1 Score: 704.9 bits (1818), Expect = 7.3e-202
Identity = 361/540 (66.85%), Postives = 424/540 (78.52%), Query Frame = 0
Query: 37 SSDDHVGSKCAMTLYPELCETTISTAVG---SSSKEAIEASVNITISAVKDNYKRVQKLL 96
SS + S C+ T YPELC + + TA G +S K+ IEASVN+TI+AV+ NY V+KL+
Sbjct: 54 SSHAVLRSSCSSTRYPELCISAVVTAGGVELTSQKDVIEASVNLTITAVEHNYFTVKKLI 113
Query: 97 KTVKNLTKRQKIAFHDCLETGEETLRELYEVVEDVNEYPKKKSLSRYADDLKTLLSSAIT 156
K K LT R+K A HDCLET +ETL EL+E VED++ YP KK+L +A DLKTL+SSAIT
Sbjct: 114 KKRKGLTPREKTALHDCLETIDETLDELHETVEDLHLYPTKKTLREHAGDLKTLISSAIT 173
Query: 157 NQETCVDGFSHDKGDKKVRESLKEGLIHIEKLCSIALALIKNLTDTDIANLNNNNNNNHL 216
NQETC+DGFSHD DK+VR++L +G IH+E +CS ALA+IKN+TDTDIAN
Sbjct: 174 NQETCLDGFSHDDADKQVRKALLKGQIHVEHMCSNALAMIKNMTDTDIANFEQKAKITSN 233
Query: 217 NRKQLEEKKME------------DGIKWPDWMSPKDRRLLQASSTATPDVVVAADGSGDF 276
NRK EE + D WP W+S DRRLLQ S D VAADGSG F
Sbjct: 234 NRKLKEENQETTVAVDIAGAGELDSEGWPTWLSAGDRRLLQGSGVKA-DATVAADGSGTF 293
Query: 277 RTISEAVAAAPSRSSRRYIIRIKAGVYRENVNVASSKRNIMFWGDGRVNTIITGNRNVVD 336
+T++ AVAAAP S++RY+I IKAGVYRENV VA K+NIMF GDGR TIITG+RNVVD
Sbjct: 294 KTVAAAVAAAPENSNKRYVIHIKAGVYRENVEVAKKKKNIMFMGDGRTRTIITGSRNVVD 353
Query: 337 GSTTFNSATVAAVGERFLARDVTFQNTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLY 396
GSTTF+SATVAAVGERFLARD+TFQNTAGPSKHQAVALRVGSD SAFY CDMLAYQDTLY
Sbjct: 354 GSTTFHSATVAAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQDTLY 413
Query: 397 VHSNRQFYVQCIIVGTIDFIFGNAAAVIQDCDIHARRPNPGQRNMVTAQGRTDPNQNTGI 456
VHSNRQF+V+C+I GT+DFIFGNAA V+QDCDIHARRPN GQ+NMVTAQGRTDPNQNTGI
Sbjct: 414 VHSNRQFFVKCLIAGTVDFIFGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGI 473
Query: 457 VIQKCRIGTTSDLRPVISNFPTFLGRPWQRYSRTVVMQTSISNVIDPAGWHVWDGNFALD 516
VIQKCRIG TSDL+ V +FPT+LGRPW+ YS+TV+MQ++IS+VI P GW W G FAL+
Sbjct: 474 VIQKCRIGATSDLQSVKGSFPTYLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFALN 533
Query: 517 TLFYAEYQNSGAGADTSRRVKWKGFRVLTRAAEAEAFTAGNFIGGGTWLSSTGFPFSLGL 562
TL Y EY N+GAGA T+ RVKW+GF+V+T AAEA+ +TAG FIGGG WLSSTGFPFSLGL
Sbjct: 534 TLTYREYSNTGAGAGTANRVKWRGFKVITAAAEAQKYTAGQFIGGGGWLSSTGFPFSLGL 592
BLAST of CSPI03G28630 vs. ExPASy Swiss-Prot
Match:
Q42534 (Pectinesterase 2 OS=Arabidopsis thaliana OX=3702 GN=PME2 PE=2 SV=2)
HSP 1 Score: 682.2 bits (1759), Expect = 5.1e-195
Identity = 351/538 (65.24%), Postives = 419/538 (77.88%), Query Frame = 0
Query: 33 LAVVSSDDH--VGSKCAMTLYPELCETTISTAVG---SSSKEAIEASVNITISAVKDNYK 92
+ +SS H + S C+ TLYPELC + ++ G +S KE IEAS+N+T AVK NY
Sbjct: 51 ITTLSSTSHAILKSVCSSTLYPELCFSAVAATGGKELTSQKEVIEASLNLTTKAVKHNYF 110
Query: 93 RVQKLLKTVKNLTKRQKIAFHDCLETGEETLRELYEVVEDVNEYPKKKSLSRYADDLKTL 152
V+KL+ K LT R+ A HDCLET +ETL EL+ VED+++YPK+KSL ++ADDLKTL
Sbjct: 111 AVKKLIAKRKGLTPREVTALHDCLETIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTL 170
Query: 153 LSSAITNQETCVDGFSHDKGDKKVRESLKEGLIHIEKLCSIALALIKNLTDTDIANL--- 212
+SSAITNQ TC+DGFS+D D+KVR++L +G +H+E +CS ALA+IKN+T+TDIAN
Sbjct: 171 ISSAITNQGTCLDGFSYDDADRKVRKALLKGQVHVEHMCSNALAMIKNMTETDIANFELR 230
Query: 213 -NNNNNNNHLNRKQLEEKKMEDGIKWPDWMSPKDRRLLQASSTATPDVVVAADGSGDFRT 272
++ N+ NRK E D WP W+S DRRLLQ ST D VA DGSGDF T
Sbjct: 231 DKSSTFTNNNNRKLKEVTGDLDSDGWPKWLSVGDRRLLQ-GSTIKADATVADDGSGDFTT 290
Query: 273 ISEAVAAAPSRSSRRYIIRIKAGVYRENVNVASSKRNIMFWGDGRVNTIITGNRNVVDGS 332
++ AVAAAP +S++R++I IKAGVYRENV V K NIMF GDGR TIITG+RNVVDGS
Sbjct: 291 VAAAVAAAPEKSNKRFVIHIKAGVYRENVEVTKKKTNIMFLGDGRGKTIITGSRNVVDGS 350
Query: 333 TTFNSATVAAVGERFLARDVTFQNTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVH 392
TTF+SATVAAVGERFLARD+TFQNTAGPSKHQAVALRVGSD SAFY+CDM AYQDTLYVH
Sbjct: 351 TTFHSATVAAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYQCDMFAYQDTLYVH 410
Query: 393 SNRQFYVQCIIVGTIDFIFGNAAAVIQDCDIHARRPNPGQRNMVTAQGRTDPNQNTGIVI 452
SNRQF+V+C I GT+DFIFGNAAAV+QDCDI+ARRPN GQ+NMVTAQGR+DPNQNTGIVI
Sbjct: 411 SNRQFFVKCHITGTVDFIFGNAAAVLQDCDINARRPNSGQKNMVTAQGRSDPNQNTGIVI 470
Query: 453 QKCRIGTTSDLRPVISNFPTFLGRPWQRYSRTVVMQTSISNVIDPAGWHVWDGNFALDTL 512
Q CRIG TSDL V FPT+LGRPW+ YSRTV+MQ+ IS+VI P GWH W G+FALDTL
Sbjct: 471 QNCRIGGTSDLLAVKGTFPTYLGRPWKEYSRTVIMQSDISDVIRPEGWHEWSGSFALDTL 530
Query: 513 FYAEYQNSGAGADTSRRVKWKGFRVLTRAAEAEAFTAGNFIGGGTWLSSTGFPFSLGL 562
Y EY N G GA T+ RVKWKG++V+T EA+ FTAG FIGGG WL+STGFPFSL L
Sbjct: 531 TYREYLNRGGGAGTANRVKWKGYKVITSDTEAQPFTAGQFIGGGGWLASTGFPFSLSL 587
BLAST of CSPI03G28630 vs. ExPASy TrEMBL
Match:
A0A0A0LCR6 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G646640 PE=3 SV=1)
HSP 1 Score: 1104.0 bits (2854), Expect = 0.0e+00
Identity = 560/561 (99.82%), Postives = 560/561 (99.82%), Query Frame = 0
Query: 1 MSKPDDTPAAGVPFSGYSKTNLLVFALSTTLLLAVVSSDDHVGSKCAMTLYPELCETTIS 60
MSKPDDTPAAGVPFSGYSKTNLLVFALSTTLLLAVVSSDDHVGSKCAMTLYPELCETTIS
Sbjct: 1 MSKPDDTPAAGVPFSGYSKTNLLVFALSTTLLLAVVSSDDHVGSKCAMTLYPELCETTIS 60
Query: 61 TAVGSSSKEAIEASVNITISAVKDNYKRVQKLLKTVKNLTKRQKIAFHDCLETGEETLRE 120
TAVGSSSKEAIEASVNITISAVKDNYKRVQKLLKTVKNLTKRQKIAFHDCLETGEETLRE
Sbjct: 61 TAVGSSSKEAIEASVNITISAVKDNYKRVQKLLKTVKNLTKRQKIAFHDCLETGEETLRE 120
Query: 121 LYEVVEDVNEYPKKKSLSRYADDLKTLLSSAITNQETCVDGFSHDKGDKKVRESLKEGLI 180
LYEVVEDVNEYPKKKSLSRYADDLKTLLSS ITNQETCVDGFSHDKGDKKVRESLKEGLI
Sbjct: 121 LYEVVEDVNEYPKKKSLSRYADDLKTLLSSTITNQETCVDGFSHDKGDKKVRESLKEGLI 180
Query: 181 HIEKLCSIALALIKNLTDTDIANLNNNNNNNHLNRKQLEEKKMEDGIKWPDWMSPKDRRL 240
HIEKLCSIALALIKNLTDTDIANLNNNNNNNHLNRKQLEEKKMEDGIKWPDWMSPKDRRL
Sbjct: 181 HIEKLCSIALALIKNLTDTDIANLNNNNNNNHLNRKQLEEKKMEDGIKWPDWMSPKDRRL 240
Query: 241 LQASSTATPDVVVAADGSGDFRTISEAVAAAPSRSSRRYIIRIKAGVYRENVNVASSKRN 300
LQASSTATPDVVVAADGSGDFRTISEAVAAAPSRSSRRYIIRIKAGVYRENVNVASSKRN
Sbjct: 241 LQASSTATPDVVVAADGSGDFRTISEAVAAAPSRSSRRYIIRIKAGVYRENVNVASSKRN 300
Query: 301 IMFWGDGRVNTIITGNRNVVDGSTTFNSATVAAVGERFLARDVTFQNTAGPSKHQAVALR 360
IMFWGDGRVNTIITGNRNVVDGSTTFNSATVAAVGERFLARDVTFQNTAGPSKHQAVALR
Sbjct: 301 IMFWGDGRVNTIITGNRNVVDGSTTFNSATVAAVGERFLARDVTFQNTAGPSKHQAVALR 360
Query: 361 VGSDLSAFYRCDMLAYQDTLYVHSNRQFYVQCIIVGTIDFIFGNAAAVIQDCDIHARRPN 420
VGSDLSAFYRCDMLAYQDTLYVHSNRQFYVQCIIVGTIDFIFGNAAAVIQDCDIHARRPN
Sbjct: 361 VGSDLSAFYRCDMLAYQDTLYVHSNRQFYVQCIIVGTIDFIFGNAAAVIQDCDIHARRPN 420
Query: 421 PGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVISNFPTFLGRPWQRYSRTVVMQT 480
PGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVISNFPTFLGRPWQRYSRTVVMQT
Sbjct: 421 PGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVISNFPTFLGRPWQRYSRTVVMQT 480
Query: 481 SISNVIDPAGWHVWDGNFALDTLFYAEYQNSGAGADTSRRVKWKGFRVLTRAAEAEAFTA 540
SISNVIDPAGWHVWDGNFALDTLFYAEYQNSGAGADTSRRVKWKGFRVLTRAAEAEAFTA
Sbjct: 481 SISNVIDPAGWHVWDGNFALDTLFYAEYQNSGAGADTSRRVKWKGFRVLTRAAEAEAFTA 540
Query: 541 GNFIGGGTWLSSTGFPFSLGL 562
GNFIGGGTWLSSTGFPFSLGL
Sbjct: 541 GNFIGGGTWLSSTGFPFSLGL 561
BLAST of CSPI03G28630 vs. ExPASy TrEMBL
Match:
A0A5A7UEV0 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold522G001090 PE=3 SV=1)
HSP 1 Score: 1039.3 bits (2686), Expect = 6.0e-300
Identity = 531/561 (94.65%), Postives = 542/561 (96.61%), Query Frame = 0
Query: 1 MSKPDDTPAAGVPFSGYSKTNLLVFALSTTLLLAVVSSDDHVGSKCAMTLYPELCETTIS 60
MSKPD+T AAGVPFS SKTNLLVFALSTTLLLA VSSDDHV SKCAMTLYPELCETTIS
Sbjct: 1 MSKPDETLAAGVPFSCNSKTNLLVFALSTTLLLAAVSSDDHVRSKCAMTLYPELCETTIS 60
Query: 61 TAVGSSSKEAIEASVNITISAVKDNYKRVQKLLKTVKNLTKRQKIAFHDCLETGEETLRE 120
TAVGSSSKEAIEASVNITI AV DNY+RV+KLLKT +LTKR+KIA HDCLETGEETLRE
Sbjct: 61 TAVGSSSKEAIEASVNITIGAVGDNYRRVKKLLKTENSLTKREKIALHDCLETGEETLRE 120
Query: 121 LYEVVEDVNEYPKKKSLSRYADDLKTLLSSAITNQETCVDGFSHDKGDKKVRESLKEGLI 180
LYEVVEDVNEYP KKSLSRYADDLKTLLSSAITNQETC+DGFSHDK DKKVRESLKEGLI
Sbjct: 121 LYEVVEDVNEYPNKKSLSRYADDLKTLLSSAITNQETCLDGFSHDKADKKVRESLKEGLI 180
Query: 181 HIEKLCSIALALIKNLTDTDIANLNNNNNNNHLNRKQLEEKKMEDGIKWPDWMSPKDRRL 240
HIEKLCSIALALIKNLTDTDIANL NNNNNHL+RKQL+EK +D I WPDWMSP DRRL
Sbjct: 181 HIEKLCSIALALIKNLTDTDIANL--NNNNNHLSRKQLKEKNTDDRINWPDWMSPNDRRL 240
Query: 241 LQASSTATPDVVVAADGSGDFRTISEAVAAAPSRSSRRYIIRIKAGVYRENVNVASSKRN 300
LQASSTATPDVVVAADGSGDFRTISEAVAAAPSRSS+RYIIRIKAGVYRENVNVASSKRN
Sbjct: 241 LQASSTATPDVVVAADGSGDFRTISEAVAAAPSRSSQRYIIRIKAGVYRENVNVASSKRN 300
Query: 301 IMFWGDGRVNTIITGNRNVVDGSTTFNSATVAAVGERFLARDVTFQNTAGPSKHQAVALR 360
IMFWGDGRVNTIITGNRNVVDGSTTFNSATVAAVGERFLARDVTFQNTAGPSKHQAVALR
Sbjct: 301 IMFWGDGRVNTIITGNRNVVDGSTTFNSATVAAVGERFLARDVTFQNTAGPSKHQAVALR 360
Query: 361 VGSDLSAFYRCDMLAYQDTLYVHSNRQFYVQCIIVGTIDFIFGNAAAVIQDCDIHARRPN 420
VGSDLSAFYRCDMLAYQDTLYVHSNRQFYVQCIIVGTIDFIFGNAAAVIQDCDIHARRPN
Sbjct: 361 VGSDLSAFYRCDMLAYQDTLYVHSNRQFYVQCIIVGTIDFIFGNAAAVIQDCDIHARRPN 420
Query: 421 PGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVISNFPTFLGRPWQRYSRTVVMQT 480
PGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVISNFPTFLGRPWQRYSRTVVMQT
Sbjct: 421 PGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVISNFPTFLGRPWQRYSRTVVMQT 480
Query: 481 SISNVIDPAGWHVWDGNFALDTLFYAEYQNSGAGADTSRRVKWKGFRVLTRAAEAEAFTA 540
SISNVIDPAGWHVWDGNFALDTLFYAEYQNSGAGADTSRRV+WKGFRVLTRAAEAEAFTA
Sbjct: 481 SISNVIDPAGWHVWDGNFALDTLFYAEYQNSGAGADTSRRVRWKGFRVLTRAAEAEAFTA 540
Query: 541 GNFIGGGTWLSSTGFPFSLGL 562
GNFIGGGTWLSSTGFPFSLGL
Sbjct: 541 GNFIGGGTWLSSTGFPFSLGL 559
BLAST of CSPI03G28630 vs. ExPASy TrEMBL
Match:
A0A1S3CCA5 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103499204 PE=3 SV=1)
HSP 1 Score: 1039.3 bits (2686), Expect = 6.0e-300
Identity = 531/561 (94.65%), Postives = 542/561 (96.61%), Query Frame = 0
Query: 1 MSKPDDTPAAGVPFSGYSKTNLLVFALSTTLLLAVVSSDDHVGSKCAMTLYPELCETTIS 60
MSKPD+T AAGVPFS SKTNLLVFALSTTLLLA VSSDDHV SKCAMTLYPELCETTIS
Sbjct: 1 MSKPDETLAAGVPFSCNSKTNLLVFALSTTLLLAAVSSDDHVRSKCAMTLYPELCETTIS 60
Query: 61 TAVGSSSKEAIEASVNITISAVKDNYKRVQKLLKTVKNLTKRQKIAFHDCLETGEETLRE 120
TAVGSSSKEAIEASVNITI AV DNY+RV+KLLKT +LTKR+KIA HDCLETGEETLRE
Sbjct: 61 TAVGSSSKEAIEASVNITIGAVGDNYRRVKKLLKTENSLTKREKIALHDCLETGEETLRE 120
Query: 121 LYEVVEDVNEYPKKKSLSRYADDLKTLLSSAITNQETCVDGFSHDKGDKKVRESLKEGLI 180
LYEVVEDVNEYP KKSLSRYADDLKTLLSSAITNQETC+DGFSHDK DKKVRESLKEGLI
Sbjct: 121 LYEVVEDVNEYPNKKSLSRYADDLKTLLSSAITNQETCLDGFSHDKADKKVRESLKEGLI 180
Query: 181 HIEKLCSIALALIKNLTDTDIANLNNNNNNNHLNRKQLEEKKMEDGIKWPDWMSPKDRRL 240
HIEKLCSIALALIKNLTDTDIANL NNNNNHL+RKQL+EK +D I WPDWMSP DRRL
Sbjct: 181 HIEKLCSIALALIKNLTDTDIANL--NNNNNHLSRKQLKEKNTDDRINWPDWMSPNDRRL 240
Query: 241 LQASSTATPDVVVAADGSGDFRTISEAVAAAPSRSSRRYIIRIKAGVYRENVNVASSKRN 300
LQASSTATPDVVVAADGSGDFRTISEAVAAAPSRSS+RYIIRIKAGVYRENVNVASSKRN
Sbjct: 241 LQASSTATPDVVVAADGSGDFRTISEAVAAAPSRSSQRYIIRIKAGVYRENVNVASSKRN 300
Query: 301 IMFWGDGRVNTIITGNRNVVDGSTTFNSATVAAVGERFLARDVTFQNTAGPSKHQAVALR 360
IMFWGDGRVNTIITGNRNVVDGSTTFNSATVAAVGERFLARDVTFQNTAGPSKHQAVALR
Sbjct: 301 IMFWGDGRVNTIITGNRNVVDGSTTFNSATVAAVGERFLARDVTFQNTAGPSKHQAVALR 360
Query: 361 VGSDLSAFYRCDMLAYQDTLYVHSNRQFYVQCIIVGTIDFIFGNAAAVIQDCDIHARRPN 420
VGSDLSAFYRCDMLAYQDTLYVHSNRQFYVQCIIVGTIDFIFGNAAAVIQDCDIHARRPN
Sbjct: 361 VGSDLSAFYRCDMLAYQDTLYVHSNRQFYVQCIIVGTIDFIFGNAAAVIQDCDIHARRPN 420
Query: 421 PGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVISNFPTFLGRPWQRYSRTVVMQT 480
PGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVISNFPTFLGRPWQRYSRTVVMQT
Sbjct: 421 PGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVISNFPTFLGRPWQRYSRTVVMQT 480
Query: 481 SISNVIDPAGWHVWDGNFALDTLFYAEYQNSGAGADTSRRVKWKGFRVLTRAAEAEAFTA 540
SISNVIDPAGWHVWDGNFALDTLFYAEYQNSGAGADTSRRV+WKGFRVLTRAAEAEAFTA
Sbjct: 481 SISNVIDPAGWHVWDGNFALDTLFYAEYQNSGAGADTSRRVRWKGFRVLTRAAEAEAFTA 540
Query: 541 GNFIGGGTWLSSTGFPFSLGL 562
GNFIGGGTWLSSTGFPFSLGL
Sbjct: 541 GNFIGGGTWLSSTGFPFSLGL 559
BLAST of CSPI03G28630 vs. ExPASy TrEMBL
Match:
A0A6J1ITZ4 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111479907 PE=3 SV=1)
HSP 1 Score: 899.0 bits (2322), Expect = 9.7e-258
Identity = 464/576 (80.56%), Postives = 498/576 (86.46%), Query Frame = 0
Query: 1 MSKPDDTPAAGVPFS------GYSKTNLLVFALSTTLLLAVVSSDDHVG---SKCAMTLY 60
M++ DD+ AG P + K NL + ALS LLL VSSDDHV S CA+TLY
Sbjct: 1 MAQTDDSTVAGAPITSSPTSISKKKHNLFILALSAALLLDGVSSDDHVAAVRSTCAVTLY 60
Query: 61 PELCETTISTAVG------SSSKEAIEASVNITISAVKDNYKRVQKLLKTVKNLTKRQKI 120
PELC++TISTAV SSSK+ IEASVNITISAVK NYK ++KL++T KNLTKR+KI
Sbjct: 61 PELCQSTISTAVETSSKKVSSSKDIIEASVNITISAVKQNYKSIKKLVETTKNLTKREKI 120
Query: 121 AFHDCLETGEETLRELYEVVEDVNEYPKKKSLSRYADDLKTLLSSAITNQETCVDGFSHD 180
A HDCLETGEETLRELYEVVED+NEYPKKKSL +YADDLKTLLSSAITNQETCVDGFSHD
Sbjct: 121 ALHDCLETGEETLRELYEVVEDLNEYPKKKSLPQYADDLKTLLSSAITNQETCVDGFSHD 180
Query: 181 KGDKKVRESLKEGLIHIEKLCSIALALIKNLTDTDIANLNNNNNNNHLNRKQLEEKKMED 240
+ DKK+RESL G IH+EKLCS+ALALIKNLTDTDIAN NN R +
Sbjct: 181 RADKKIRESLLAGQIHVEKLCSVALALIKNLTDTDIANHYKLTGNNIQGRSMAMSE---- 240
Query: 241 GIKWPDWMSPKDRRLLQASSTATPDVVVAADGSGDFRTISEAVAAAPSRSSRRYIIRIKA 300
WPDWMSP DR+LLQASSTATPDVVVAADGSGDF+T+S AVAAAPSRSSRRYIIRIKA
Sbjct: 241 -FNWPDWMSPGDRKLLQASSTATPDVVVAADGSGDFQTVSAAVAAAPSRSSRRYIIRIKA 300
Query: 301 GVYRENVNVASSKRNIMFWGDGRVNTIITGNRNVVDGSTTFNSATVAAVGERFLARDVTF 360
GVYRENVNV SSK NIMFWGDGR TIITGNRNVVDGSTTFNSATVAAVGERFLARD+TF
Sbjct: 301 GVYRENVNVPSSKTNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGERFLARDITF 360
Query: 361 QNTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVQCIIVGTIDFIFGNA 420
QNTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYV CIIVGT+DFIFGNA
Sbjct: 361 QNTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVSCIIVGTVDFIFGNA 420
Query: 421 AAVIQDCDIHARRPNPGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVISNFPTFL 480
AAVIQ+ DIHARRPNPGQ+NMVTAQGRTDPNQNTGIVIQKCRIGTTSDL+PVI NFPTFL
Sbjct: 421 AAVIQNSDIHARRPNPGQKNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLQPVIRNFPTFL 480
Query: 481 GRPWQRYSRTVVMQTSISNVIDPAGWHVWDGNFALDTLFYAEYQNSGAGADTSRRVKWKG 540
GRPWQRYSRTVVMQTSISNVIDPAGWH+WDGNFALDTLFYAE+QNSGAGADTSRRV WKG
Sbjct: 481 GRPWQRYSRTVVMQTSISNVIDPAGWHIWDGNFALDTLFYAEFQNSGAGADTSRRVTWKG 540
Query: 541 FRVLTRAAEAEAFTAGNFIGGGTWLSSTGFPFSLGL 562
+RV++ AAEAE FTAG FI GGTWLSSTGFPFSLGL
Sbjct: 541 YRVISSAAEAEGFTAGKFISGGTWLSSTGFPFSLGL 571
BLAST of CSPI03G28630 vs. ExPASy TrEMBL
Match:
A0A6J1GV42 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111457458 PE=3 SV=1)
HSP 1 Score: 893.3 bits (2307), Expect = 5.3e-256
Identity = 461/576 (80.03%), Postives = 497/576 (86.28%), Query Frame = 0
Query: 1 MSKPDDTPAAGVPFSG------YSKTNLLVFALSTTLLLAVVSSDDH---VGSKCAMTLY 60
M++ DD+ AG P +G K NL + LS LLLA VSS+DH V S CA+TLY
Sbjct: 1 MAQTDDSTVAGAPITGCPTSISNKKHNLFIIVLSAALLLAGVSSEDHVTAVRSTCAVTLY 60
Query: 61 PELCETTISTAVG------SSSKEAIEASVNITISAVKDNYKRVQKLLKTVKNLTKRQKI 120
PELC++TISTAV SSSK+ IEASVNITISAVK NYK ++KLL+T K+LTKR+KI
Sbjct: 61 PELCQSTISTAVETSSKKVSSSKDIIEASVNITISAVKQNYKSIKKLLETTKHLTKREKI 120
Query: 121 AFHDCLETGEETLRELYEVVEDVNEYPKKKSLSRYADDLKTLLSSAITNQETCVDGFSHD 180
A HDCLETGEETLRELYEVVED+NEYPKKKSL +YADDLKTLLSSAITNQETCVDGFSHD
Sbjct: 121 ALHDCLETGEETLRELYEVVEDLNEYPKKKSLPQYADDLKTLLSSAITNQETCVDGFSHD 180
Query: 181 KGDKKVRESLKEGLIHIEKLCSIALALIKNLTDTDIANLNNNNNNNHLNRKQLEEKKMED 240
+ DKKVRESL G H+EKLCS+ALALIKNLTDTDIA NH N + E
Sbjct: 181 RADKKVRESLLAGQFHVEKLCSVALALIKNLTDTDIA--------NHYNIRGRSMAMSE- 240
Query: 241 GIKWPDWMSPKDRRLLQASSTATPDVVVAADGSGDFRTISEAVAAAPSRSSRRYIIRIKA 300
WPDWMSP DR+LLQASSTATPD VVAADGSGDF+T+S AVAAAPSRSSRRYIIRIKA
Sbjct: 241 -FNWPDWMSPGDRKLLQASSTATPDAVVAADGSGDFQTVSAAVAAAPSRSSRRYIIRIKA 300
Query: 301 GVYRENVNVASSKRNIMFWGDGRVNTIITGNRNVVDGSTTFNSATVAAVGERFLARDVTF 360
GVYRENVNV SSK NIMFWGDGR TIITGNRNVVDGSTTFNSATVAAVG RFLARD+TF
Sbjct: 301 GVYRENVNVPSSKTNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGGRFLARDITF 360
Query: 361 QNTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVQCIIVGTIDFIFGNA 420
QNTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYV CIIVGT+DFIFGNA
Sbjct: 361 QNTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVSCIIVGTVDFIFGNA 420
Query: 421 AAVIQDCDIHARRPNPGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVISNFPTFL 480
AAVIQ+ DIHARRPNPGQ+NMVTAQGRTDPNQNTGIVIQKCRIGTTSDL+PVI NFPTFL
Sbjct: 421 AAVIQNSDIHARRPNPGQKNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLQPVIRNFPTFL 480
Query: 481 GRPWQRYSRTVVMQTSISNVIDPAGWHVWDGNFALDTLFYAEYQNSGAGADTSRRVKWKG 540
GRPWQRYSRTVVMQTSISNVIDPAGWH+WDGNFALDTLFYAEYQN+GAGADTSRRV WKG
Sbjct: 481 GRPWQRYSRTVVMQTSISNVIDPAGWHIWDGNFALDTLFYAEYQNNGAGADTSRRVTWKG 540
Query: 541 FRVLTRAAEAEAFTAGNFIGGGTWLSSTGFPFSLGL 562
+R+++ AAEAE+FTAG FI GGTWLSSTGFPFSLGL
Sbjct: 541 YRIISSAAEAESFTAGKFISGGTWLSSTGFPFSLGL 566
BLAST of CSPI03G28630 vs. NCBI nr
Match:
XP_004144447.2 (pectinesterase [Cucumis sativus] >KGN58477.1 hypothetical protein Csa_002294 [Cucumis sativus])
HSP 1 Score: 1104.0 bits (2854), Expect = 0.0e+00
Identity = 560/561 (99.82%), Postives = 560/561 (99.82%), Query Frame = 0
Query: 1 MSKPDDTPAAGVPFSGYSKTNLLVFALSTTLLLAVVSSDDHVGSKCAMTLYPELCETTIS 60
MSKPDDTPAAGVPFSGYSKTNLLVFALSTTLLLAVVSSDDHVGSKCAMTLYPELCETTIS
Sbjct: 1 MSKPDDTPAAGVPFSGYSKTNLLVFALSTTLLLAVVSSDDHVGSKCAMTLYPELCETTIS 60
Query: 61 TAVGSSSKEAIEASVNITISAVKDNYKRVQKLLKTVKNLTKRQKIAFHDCLETGEETLRE 120
TAVGSSSKEAIEASVNITISAVKDNYKRVQKLLKTVKNLTKRQKIAFHDCLETGEETLRE
Sbjct: 61 TAVGSSSKEAIEASVNITISAVKDNYKRVQKLLKTVKNLTKRQKIAFHDCLETGEETLRE 120
Query: 121 LYEVVEDVNEYPKKKSLSRYADDLKTLLSSAITNQETCVDGFSHDKGDKKVRESLKEGLI 180
LYEVVEDVNEYPKKKSLSRYADDLKTLLSS ITNQETCVDGFSHDKGDKKVRESLKEGLI
Sbjct: 121 LYEVVEDVNEYPKKKSLSRYADDLKTLLSSTITNQETCVDGFSHDKGDKKVRESLKEGLI 180
Query: 181 HIEKLCSIALALIKNLTDTDIANLNNNNNNNHLNRKQLEEKKMEDGIKWPDWMSPKDRRL 240
HIEKLCSIALALIKNLTDTDIANLNNNNNNNHLNRKQLEEKKMEDGIKWPDWMSPKDRRL
Sbjct: 181 HIEKLCSIALALIKNLTDTDIANLNNNNNNNHLNRKQLEEKKMEDGIKWPDWMSPKDRRL 240
Query: 241 LQASSTATPDVVVAADGSGDFRTISEAVAAAPSRSSRRYIIRIKAGVYRENVNVASSKRN 300
LQASSTATPDVVVAADGSGDFRTISEAVAAAPSRSSRRYIIRIKAGVYRENVNVASSKRN
Sbjct: 241 LQASSTATPDVVVAADGSGDFRTISEAVAAAPSRSSRRYIIRIKAGVYRENVNVASSKRN 300
Query: 301 IMFWGDGRVNTIITGNRNVVDGSTTFNSATVAAVGERFLARDVTFQNTAGPSKHQAVALR 360
IMFWGDGRVNTIITGNRNVVDGSTTFNSATVAAVGERFLARDVTFQNTAGPSKHQAVALR
Sbjct: 301 IMFWGDGRVNTIITGNRNVVDGSTTFNSATVAAVGERFLARDVTFQNTAGPSKHQAVALR 360
Query: 361 VGSDLSAFYRCDMLAYQDTLYVHSNRQFYVQCIIVGTIDFIFGNAAAVIQDCDIHARRPN 420
VGSDLSAFYRCDMLAYQDTLYVHSNRQFYVQCIIVGTIDFIFGNAAAVIQDCDIHARRPN
Sbjct: 361 VGSDLSAFYRCDMLAYQDTLYVHSNRQFYVQCIIVGTIDFIFGNAAAVIQDCDIHARRPN 420
Query: 421 PGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVISNFPTFLGRPWQRYSRTVVMQT 480
PGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVISNFPTFLGRPWQRYSRTVVMQT
Sbjct: 421 PGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVISNFPTFLGRPWQRYSRTVVMQT 480
Query: 481 SISNVIDPAGWHVWDGNFALDTLFYAEYQNSGAGADTSRRVKWKGFRVLTRAAEAEAFTA 540
SISNVIDPAGWHVWDGNFALDTLFYAEYQNSGAGADTSRRVKWKGFRVLTRAAEAEAFTA
Sbjct: 481 SISNVIDPAGWHVWDGNFALDTLFYAEYQNSGAGADTSRRVKWKGFRVLTRAAEAEAFTA 540
Query: 541 GNFIGGGTWLSSTGFPFSLGL 562
GNFIGGGTWLSSTGFPFSLGL
Sbjct: 541 GNFIGGGTWLSSTGFPFSLGL 561
BLAST of CSPI03G28630 vs. NCBI nr
Match:
XP_008460358.1 (PREDICTED: pectinesterase-like [Cucumis melo] >KAA0053588.1 pectinesterase-like [Cucumis melo var. makuwa] >TYK19178.1 pectinesterase-like [Cucumis melo var. makuwa])
HSP 1 Score: 1039.3 bits (2686), Expect = 1.2e-299
Identity = 531/561 (94.65%), Postives = 542/561 (96.61%), Query Frame = 0
Query: 1 MSKPDDTPAAGVPFSGYSKTNLLVFALSTTLLLAVVSSDDHVGSKCAMTLYPELCETTIS 60
MSKPD+T AAGVPFS SKTNLLVFALSTTLLLA VSSDDHV SKCAMTLYPELCETTIS
Sbjct: 1 MSKPDETLAAGVPFSCNSKTNLLVFALSTTLLLAAVSSDDHVRSKCAMTLYPELCETTIS 60
Query: 61 TAVGSSSKEAIEASVNITISAVKDNYKRVQKLLKTVKNLTKRQKIAFHDCLETGEETLRE 120
TAVGSSSKEAIEASVNITI AV DNY+RV+KLLKT +LTKR+KIA HDCLETGEETLRE
Sbjct: 61 TAVGSSSKEAIEASVNITIGAVGDNYRRVKKLLKTENSLTKREKIALHDCLETGEETLRE 120
Query: 121 LYEVVEDVNEYPKKKSLSRYADDLKTLLSSAITNQETCVDGFSHDKGDKKVRESLKEGLI 180
LYEVVEDVNEYP KKSLSRYADDLKTLLSSAITNQETC+DGFSHDK DKKVRESLKEGLI
Sbjct: 121 LYEVVEDVNEYPNKKSLSRYADDLKTLLSSAITNQETCLDGFSHDKADKKVRESLKEGLI 180
Query: 181 HIEKLCSIALALIKNLTDTDIANLNNNNNNNHLNRKQLEEKKMEDGIKWPDWMSPKDRRL 240
HIEKLCSIALALIKNLTDTDIANL NNNNNHL+RKQL+EK +D I WPDWMSP DRRL
Sbjct: 181 HIEKLCSIALALIKNLTDTDIANL--NNNNNHLSRKQLKEKNTDDRINWPDWMSPNDRRL 240
Query: 241 LQASSTATPDVVVAADGSGDFRTISEAVAAAPSRSSRRYIIRIKAGVYRENVNVASSKRN 300
LQASSTATPDVVVAADGSGDFRTISEAVAAAPSRSS+RYIIRIKAGVYRENVNVASSKRN
Sbjct: 241 LQASSTATPDVVVAADGSGDFRTISEAVAAAPSRSSQRYIIRIKAGVYRENVNVASSKRN 300
Query: 301 IMFWGDGRVNTIITGNRNVVDGSTTFNSATVAAVGERFLARDVTFQNTAGPSKHQAVALR 360
IMFWGDGRVNTIITGNRNVVDGSTTFNSATVAAVGERFLARDVTFQNTAGPSKHQAVALR
Sbjct: 301 IMFWGDGRVNTIITGNRNVVDGSTTFNSATVAAVGERFLARDVTFQNTAGPSKHQAVALR 360
Query: 361 VGSDLSAFYRCDMLAYQDTLYVHSNRQFYVQCIIVGTIDFIFGNAAAVIQDCDIHARRPN 420
VGSDLSAFYRCDMLAYQDTLYVHSNRQFYVQCIIVGTIDFIFGNAAAVIQDCDIHARRPN
Sbjct: 361 VGSDLSAFYRCDMLAYQDTLYVHSNRQFYVQCIIVGTIDFIFGNAAAVIQDCDIHARRPN 420
Query: 421 PGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVISNFPTFLGRPWQRYSRTVVMQT 480
PGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVISNFPTFLGRPWQRYSRTVVMQT
Sbjct: 421 PGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVISNFPTFLGRPWQRYSRTVVMQT 480
Query: 481 SISNVIDPAGWHVWDGNFALDTLFYAEYQNSGAGADTSRRVKWKGFRVLTRAAEAEAFTA 540
SISNVIDPAGWHVWDGNFALDTLFYAEYQNSGAGADTSRRV+WKGFRVLTRAAEAEAFTA
Sbjct: 481 SISNVIDPAGWHVWDGNFALDTLFYAEYQNSGAGADTSRRVRWKGFRVLTRAAEAEAFTA 540
Query: 541 GNFIGGGTWLSSTGFPFSLGL 562
GNFIGGGTWLSSTGFPFSLGL
Sbjct: 541 GNFIGGGTWLSSTGFPFSLGL 559
BLAST of CSPI03G28630 vs. NCBI nr
Match:
XP_038878011.1 (pectinesterase-like [Benincasa hispida])
HSP 1 Score: 920.6 bits (2378), Expect = 6.4e-264
Identity = 479/575 (83.30%), Postives = 516/575 (89.74%), Query Frame = 0
Query: 1 MSKPDDTPAAGVPFSGYS------KTNLLVFALSTTLLLA-VVSSDDHVGSKCAMTLYPE 60
M++ DD+ AAGV FSG K+ LLV A+S TLL+A V SSD+ V S CA+TLYPE
Sbjct: 1 MAQTDDSAAAGVSFSGRPISISNIKSKLLVLAMSATLLVAGVSSSDEQVRSTCAVTLYPE 60
Query: 61 LCETTISTAVGS--SSKEAIEASVNITISAVKDNYKRVQKLLKTVKNLTKRQKIAFHDCL 120
LCE TIST GS SSK+AIEASVNITI AVKDNYKR++KL+KTVKNLTKR+KIA +DCL
Sbjct: 61 LCEKTISTGGGSRWSSKDAIEASVNITIGAVKDNYKRMEKLVKTVKNLTKREKIALNDCL 120
Query: 121 ETGEETLRELYEVVEDVNEYPKKKSLSRYADDLKTLLSSAITNQETCVDGFSHDKGDKKV 180
E+ EETLRELYEVVED++EYP KKSL++YADDLKTLLSSAITNQETCVDGFSHDK DKKV
Sbjct: 121 ESEEETLRELYEVVEDLHEYPNKKSLTQYADDLKTLLSSAITNQETCVDGFSHDKADKKV 180
Query: 181 RESLKEGLIHIEKLCSIALALIKNLTDTDIANLNNNNNNNHLNRKQLEE-----KKMEDG 240
RES++ GLIHI+KLCSIALALIKNLTDTDIA N+N N LNRK L E + +D
Sbjct: 181 RESIEAGLIHIKKLCSIALALIKNLTDTDIA----NHNYNVLNRKHLAEVNNGGRGNDDR 240
Query: 241 IKWPDWMSPKDRRLLQASSTATPDVVVAADGSGDFRTISEAVAAAPSRSSRRYIIRIKAG 300
I WPDWMS DRRLLQASSTATPDVVVAADG+G+FRT+SEAVAAAPSRSSRRYIIRIKAG
Sbjct: 241 INWPDWMSSGDRRLLQASSTATPDVVVAADGTGNFRTVSEAVAAAPSRSSRRYIIRIKAG 300
Query: 301 VYRENVNVASSKRNIMFWGDGRVNTIITGNRNVVDGSTTFNSATVAAVGERFLARDVTFQ 360
VYRENVNV SSK N+MFWGDGR TIITGNRNVVDGSTTFNSATVAAVG RFLARD+TFQ
Sbjct: 301 VYRENVNVPSSKSNLMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGARFLARDITFQ 360
Query: 361 NTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVQCIIVGTIDFIFGNAA 420
NTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFY++CIIVGTIDFIFGNAA
Sbjct: 361 NTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYIKCIIVGTIDFIFGNAA 420
Query: 421 AVIQDCDIHARRPNPGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVISNFPTFLG 480
AVIQDCDIHARRPNPGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDL+ VI NFPTFLG
Sbjct: 421 AVIQDCDIHARRPNPGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLQQVIGNFPTFLG 480
Query: 481 RPWQRYSRTVVMQTSISNVIDPAGWHVWDGNFALDTLFYAEYQNSGAGADTSRRVKWKGF 540
RPWQRYSRTVVMQTSISNVIDPAGWHVW+GNFALDTLFYAEYQNSGAGADTSRRV WKG+
Sbjct: 481 RPWQRYSRTVVMQTSISNVIDPAGWHVWNGNFALDTLFYAEYQNSGAGADTSRRVTWKGY 540
Query: 541 RVLTRAAEAEAFTAGNFIGGGTWLSSTGFPFSLGL 562
RV+T AAEA+AFTAGNFI GGTWLSSTGFPFSLGL
Sbjct: 541 RVITSAAEAQAFTAGNFISGGTWLSSTGFPFSLGL 571
BLAST of CSPI03G28630 vs. NCBI nr
Match:
XP_022980581.1 (pectinesterase-like [Cucurbita maxima])
HSP 1 Score: 899.0 bits (2322), Expect = 2.0e-257
Identity = 464/576 (80.56%), Postives = 498/576 (86.46%), Query Frame = 0
Query: 1 MSKPDDTPAAGVPFS------GYSKTNLLVFALSTTLLLAVVSSDDHVG---SKCAMTLY 60
M++ DD+ AG P + K NL + ALS LLL VSSDDHV S CA+TLY
Sbjct: 1 MAQTDDSTVAGAPITSSPTSISKKKHNLFILALSAALLLDGVSSDDHVAAVRSTCAVTLY 60
Query: 61 PELCETTISTAVG------SSSKEAIEASVNITISAVKDNYKRVQKLLKTVKNLTKRQKI 120
PELC++TISTAV SSSK+ IEASVNITISAVK NYK ++KL++T KNLTKR+KI
Sbjct: 61 PELCQSTISTAVETSSKKVSSSKDIIEASVNITISAVKQNYKSIKKLVETTKNLTKREKI 120
Query: 121 AFHDCLETGEETLRELYEVVEDVNEYPKKKSLSRYADDLKTLLSSAITNQETCVDGFSHD 180
A HDCLETGEETLRELYEVVED+NEYPKKKSL +YADDLKTLLSSAITNQETCVDGFSHD
Sbjct: 121 ALHDCLETGEETLRELYEVVEDLNEYPKKKSLPQYADDLKTLLSSAITNQETCVDGFSHD 180
Query: 181 KGDKKVRESLKEGLIHIEKLCSIALALIKNLTDTDIANLNNNNNNNHLNRKQLEEKKMED 240
+ DKK+RESL G IH+EKLCS+ALALIKNLTDTDIAN NN R +
Sbjct: 181 RADKKIRESLLAGQIHVEKLCSVALALIKNLTDTDIANHYKLTGNNIQGRSMAMSE---- 240
Query: 241 GIKWPDWMSPKDRRLLQASSTATPDVVVAADGSGDFRTISEAVAAAPSRSSRRYIIRIKA 300
WPDWMSP DR+LLQASSTATPDVVVAADGSGDF+T+S AVAAAPSRSSRRYIIRIKA
Sbjct: 241 -FNWPDWMSPGDRKLLQASSTATPDVVVAADGSGDFQTVSAAVAAAPSRSSRRYIIRIKA 300
Query: 301 GVYRENVNVASSKRNIMFWGDGRVNTIITGNRNVVDGSTTFNSATVAAVGERFLARDVTF 360
GVYRENVNV SSK NIMFWGDGR TIITGNRNVVDGSTTFNSATVAAVGERFLARD+TF
Sbjct: 301 GVYRENVNVPSSKTNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGERFLARDITF 360
Query: 361 QNTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVQCIIVGTIDFIFGNA 420
QNTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYV CIIVGT+DFIFGNA
Sbjct: 361 QNTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVSCIIVGTVDFIFGNA 420
Query: 421 AAVIQDCDIHARRPNPGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVISNFPTFL 480
AAVIQ+ DIHARRPNPGQ+NMVTAQGRTDPNQNTGIVIQKCRIGTTSDL+PVI NFPTFL
Sbjct: 421 AAVIQNSDIHARRPNPGQKNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLQPVIRNFPTFL 480
Query: 481 GRPWQRYSRTVVMQTSISNVIDPAGWHVWDGNFALDTLFYAEYQNSGAGADTSRRVKWKG 540
GRPWQRYSRTVVMQTSISNVIDPAGWH+WDGNFALDTLFYAE+QNSGAGADTSRRV WKG
Sbjct: 481 GRPWQRYSRTVVMQTSISNVIDPAGWHIWDGNFALDTLFYAEFQNSGAGADTSRRVTWKG 540
Query: 541 FRVLTRAAEAEAFTAGNFIGGGTWLSSTGFPFSLGL 562
+RV++ AAEAE FTAG FI GGTWLSSTGFPFSLGL
Sbjct: 541 YRVISSAAEAEGFTAGKFISGGTWLSSTGFPFSLGL 571
BLAST of CSPI03G28630 vs. NCBI nr
Match:
XP_023526589.1 (pectinesterase-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 898.7 bits (2321), Expect = 2.6e-257
Identity = 465/576 (80.73%), Postives = 500/576 (86.81%), Query Frame = 0
Query: 1 MSKPDDTPAAGVPFSG------YSKTNLLVFALSTTLLLAVVSSDDH---VGSKCAMTLY 60
M++ DD+ AG P +G K NL + ALS +LLA VSSDDH V S CA+TLY
Sbjct: 1 MAQTDDSTVAGAPSTGSPTSISNKKHNLFILALSAAVLLAGVSSDDHVTAVRSTCAVTLY 60
Query: 61 PELCETTISTAVG------SSSKEAIEASVNITISAVKDNYKRVQKLLKTVKNLTKRQKI 120
PELC++TISTAV SSSK+ IEASVNITISAVK NYK ++KLL+T K+LTKR+KI
Sbjct: 61 PELCQSTISTAVETSSKKVSSSKDIIEASVNITISAVKQNYKSIKKLLETTKHLTKREKI 120
Query: 121 AFHDCLETGEETLRELYEVVEDVNEYPKKKSLSRYADDLKTLLSSAITNQETCVDGFSHD 180
A HDCLETGEETLRELYEVVED+NEYPKKKSL +YADDLKTLLSSAITNQETCVDGFSHD
Sbjct: 121 ALHDCLETGEETLRELYEVVEDLNEYPKKKSLPQYADDLKTLLSSAITNQETCVDGFSHD 180
Query: 181 KGDKKVRESLKEGLIHIEKLCSIALALIKNLTDTDIANLNNNNNNNHLNRKQLEEKKMED 240
+ DKK+RESL G IH+EKLCS+ALALIKNLTDTDIA NH N + E
Sbjct: 181 RADKKIRESLLAGQIHVEKLCSVALALIKNLTDTDIA--------NHYNIRGRSMAMSE- 240
Query: 241 GIKWPDWMSPKDRRLLQASSTATPDVVVAADGSGDFRTISEAVAAAPSRSSRRYIIRIKA 300
WPDWMSP DRRLLQASSTATPDVVVAADGSGDF+T+S AVAAAPSRSSRRYIIRIKA
Sbjct: 241 -FNWPDWMSPGDRRLLQASSTATPDVVVAADGSGDFQTVSAAVAAAPSRSSRRYIIRIKA 300
Query: 301 GVYRENVNVASSKRNIMFWGDGRVNTIITGNRNVVDGSTTFNSATVAAVGERFLARDVTF 360
GVYRENVNV SSK NIMFWGDGR TIITGNRNVVDGSTTFNSATVAAVG RFLARD+TF
Sbjct: 301 GVYRENVNVPSSKTNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGGRFLARDITF 360
Query: 361 QNTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVQCIIVGTIDFIFGNA 420
QNTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYV CIIVGT+DFIFGNA
Sbjct: 361 QNTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVSCIIVGTVDFIFGNA 420
Query: 421 AAVIQDCDIHARRPNPGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVISNFPTFL 480
AAVIQ+ DIHARRPNPGQ+NMVTAQGRTDPNQNTGIVIQKCRIGTTSDL+PVI NFPTFL
Sbjct: 421 AAVIQNSDIHARRPNPGQKNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLQPVIRNFPTFL 480
Query: 481 GRPWQRYSRTVVMQTSISNVIDPAGWHVWDGNFALDTLFYAEYQNSGAGADTSRRVKWKG 540
GRPWQRYSRTVVMQTSISNVIDPAGWH+WDGNFALDTLFYAEYQNSGAGADTSRRV WKG
Sbjct: 481 GRPWQRYSRTVVMQTSISNVIDPAGWHIWDGNFALDTLFYAEYQNSGAGADTSRRVTWKG 540
Query: 541 FRVLTRAAEAEAFTAGNFIGGGTWLSSTGFPFSLGL 562
+RV++ AA+AE+FTAG FI GGTWLSSTGFPFSLGL
Sbjct: 541 YRVISSAADAESFTAGKFISGGTWLSSTGFPFSLGL 566
BLAST of CSPI03G28630 vs. TAIR 10
Match:
AT3G14310.1 (pectin methylesterase 3 )
HSP 1 Score: 704.9 bits (1818), Expect = 5.2e-203
Identity = 361/540 (66.85%), Postives = 424/540 (78.52%), Query Frame = 0
Query: 37 SSDDHVGSKCAMTLYPELCETTISTAVG---SSSKEAIEASVNITISAVKDNYKRVQKLL 96
SS + S C+ T YPELC + + TA G +S K+ IEASVN+TI+AV+ NY V+KL+
Sbjct: 54 SSHAVLRSSCSSTRYPELCISAVVTAGGVELTSQKDVIEASVNLTITAVEHNYFTVKKLI 113
Query: 97 KTVKNLTKRQKIAFHDCLETGEETLRELYEVVEDVNEYPKKKSLSRYADDLKTLLSSAIT 156
K K LT R+K A HDCLET +ETL EL+E VED++ YP KK+L +A DLKTL+SSAIT
Sbjct: 114 KKRKGLTPREKTALHDCLETIDETLDELHETVEDLHLYPTKKTLREHAGDLKTLISSAIT 173
Query: 157 NQETCVDGFSHDKGDKKVRESLKEGLIHIEKLCSIALALIKNLTDTDIANLNNNNNNNHL 216
NQETC+DGFSHD DK+VR++L +G IH+E +CS ALA+IKN+TDTDIAN
Sbjct: 174 NQETCLDGFSHDDADKQVRKALLKGQIHVEHMCSNALAMIKNMTDTDIANFEQKAKITSN 233
Query: 217 NRKQLEEKKME------------DGIKWPDWMSPKDRRLLQASSTATPDVVVAADGSGDF 276
NRK EE + D WP W+S DRRLLQ S D VAADGSG F
Sbjct: 234 NRKLKEENQETTVAVDIAGAGELDSEGWPTWLSAGDRRLLQGSGVKA-DATVAADGSGTF 293
Query: 277 RTISEAVAAAPSRSSRRYIIRIKAGVYRENVNVASSKRNIMFWGDGRVNTIITGNRNVVD 336
+T++ AVAAAP S++RY+I IKAGVYRENV VA K+NIMF GDGR TIITG+RNVVD
Sbjct: 294 KTVAAAVAAAPENSNKRYVIHIKAGVYRENVEVAKKKKNIMFMGDGRTRTIITGSRNVVD 353
Query: 337 GSTTFNSATVAAVGERFLARDVTFQNTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLY 396
GSTTF+SATVAAVGERFLARD+TFQNTAGPSKHQAVALRVGSD SAFY CDMLAYQDTLY
Sbjct: 354 GSTTFHSATVAAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQDTLY 413
Query: 397 VHSNRQFYVQCIIVGTIDFIFGNAAAVIQDCDIHARRPNPGQRNMVTAQGRTDPNQNTGI 456
VHSNRQF+V+C+I GT+DFIFGNAA V+QDCDIHARRPN GQ+NMVTAQGRTDPNQNTGI
Sbjct: 414 VHSNRQFFVKCLIAGTVDFIFGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGI 473
Query: 457 VIQKCRIGTTSDLRPVISNFPTFLGRPWQRYSRTVVMQTSISNVIDPAGWHVWDGNFALD 516
VIQKCRIG TSDL+ V +FPT+LGRPW+ YS+TV+MQ++IS+VI P GW W G FAL+
Sbjct: 474 VIQKCRIGATSDLQSVKGSFPTYLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFALN 533
Query: 517 TLFYAEYQNSGAGADTSRRVKWKGFRVLTRAAEAEAFTAGNFIGGGTWLSSTGFPFSLGL 562
TL Y EY N+GAGA T+ RVKW+GF+V+T AAEA+ +TAG FIGGG WLSSTGFPFSLGL
Sbjct: 534 TLTYREYSNTGAGAGTANRVKWRGFKVITAAAEAQKYTAGQFIGGGGWLSSTGFPFSLGL 592
BLAST of CSPI03G28630 vs. TAIR 10
Match:
AT1G53830.1 (pectin methylesterase 2 )
HSP 1 Score: 682.2 bits (1759), Expect = 3.6e-196
Identity = 351/538 (65.24%), Postives = 419/538 (77.88%), Query Frame = 0
Query: 33 LAVVSSDDH--VGSKCAMTLYPELCETTISTAVG---SSSKEAIEASVNITISAVKDNYK 92
+ +SS H + S C+ TLYPELC + ++ G +S KE IEAS+N+T AVK NY
Sbjct: 51 ITTLSSTSHAILKSVCSSTLYPELCFSAVAATGGKELTSQKEVIEASLNLTTKAVKHNYF 110
Query: 93 RVQKLLKTVKNLTKRQKIAFHDCLETGEETLRELYEVVEDVNEYPKKKSLSRYADDLKTL 152
V+KL+ K LT R+ A HDCLET +ETL EL+ VED+++YPK+KSL ++ADDLKTL
Sbjct: 111 AVKKLIAKRKGLTPREVTALHDCLETIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTL 170
Query: 153 LSSAITNQETCVDGFSHDKGDKKVRESLKEGLIHIEKLCSIALALIKNLTDTDIANL--- 212
+SSAITNQ TC+DGFS+D D+KVR++L +G +H+E +CS ALA+IKN+T+TDIAN
Sbjct: 171 ISSAITNQGTCLDGFSYDDADRKVRKALLKGQVHVEHMCSNALAMIKNMTETDIANFELR 230
Query: 213 -NNNNNNNHLNRKQLEEKKMEDGIKWPDWMSPKDRRLLQASSTATPDVVVAADGSGDFRT 272
++ N+ NRK E D WP W+S DRRLLQ ST D VA DGSGDF T
Sbjct: 231 DKSSTFTNNNNRKLKEVTGDLDSDGWPKWLSVGDRRLLQ-GSTIKADATVADDGSGDFTT 290
Query: 273 ISEAVAAAPSRSSRRYIIRIKAGVYRENVNVASSKRNIMFWGDGRVNTIITGNRNVVDGS 332
++ AVAAAP +S++R++I IKAGVYRENV V K NIMF GDGR TIITG+RNVVDGS
Sbjct: 291 VAAAVAAAPEKSNKRFVIHIKAGVYRENVEVTKKKTNIMFLGDGRGKTIITGSRNVVDGS 350
Query: 333 TTFNSATVAAVGERFLARDVTFQNTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVH 392
TTF+SATVAAVGERFLARD+TFQNTAGPSKHQAVALRVGSD SAFY+CDM AYQDTLYVH
Sbjct: 351 TTFHSATVAAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYQCDMFAYQDTLYVH 410
Query: 393 SNRQFYVQCIIVGTIDFIFGNAAAVIQDCDIHARRPNPGQRNMVTAQGRTDPNQNTGIVI 452
SNRQF+V+C I GT+DFIFGNAAAV+QDCDI+ARRPN GQ+NMVTAQGR+DPNQNTGIVI
Sbjct: 411 SNRQFFVKCHITGTVDFIFGNAAAVLQDCDINARRPNSGQKNMVTAQGRSDPNQNTGIVI 470
Query: 453 QKCRIGTTSDLRPVISNFPTFLGRPWQRYSRTVVMQTSISNVIDPAGWHVWDGNFALDTL 512
Q CRIG TSDL V FPT+LGRPW+ YSRTV+MQ+ IS+VI P GWH W G+FALDTL
Sbjct: 471 QNCRIGGTSDLLAVKGTFPTYLGRPWKEYSRTVIMQSDISDVIRPEGWHEWSGSFALDTL 530
Query: 513 FYAEYQNSGAGADTSRRVKWKGFRVLTRAAEAEAFTAGNFIGGGTWLSSTGFPFSLGL 562
Y EY N G GA T+ RVKWKG++V+T EA+ FTAG FIGGG WL+STGFPFSL L
Sbjct: 531 TYREYLNRGGGAGTANRVKWKGYKVITSDTEAQPFTAGQFIGGGGWLASTGFPFSLSL 587
BLAST of CSPI03G28630 vs. TAIR 10
Match:
AT1G11580.1 (methylesterase PCR A )
HSP 1 Score: 414.1 bits (1063), Expect = 1.8e-115
Identity = 222/459 (48.37%), Postives = 295/459 (64.27%), Query Frame = 0
Query: 102 RQKIAFHDCLETGEETLRELYEVVEDVNEYPKKKSLSRYADDLKTLLSSAITNQETCVDG 161
R K F DC E + + + +E++ +L Y+ ++ T LSS +TN TC++
Sbjct: 118 RDKAGFADCEEMMDVSKDRMMSSMEELR--GGNYNLESYS-NVHTWLSSVLTNYMTCLES 177
Query: 162 FSHDKGDKKVRESLKEGLIHIEKLCSIALALIKNLTDTDIANLNNNNNNNHLNRKQLEEK 221
S + K + +K L + +ALA+ ++ ++
Sbjct: 178 ISDVSVNSK--QIVKPQLEDLVSRARVALAIFVSVLPA------------------RDDL 237
Query: 222 KMEDGIKWPDWMSPKDRRLLQASS---TATPDVVVAADGSGDFRTISEAVAAAPSRSSRR 281
KM ++P W++ DR+LL++S T +VVVA DG+G F+T++EAVAAAP S+ R
Sbjct: 238 KMIISNRFPSWLTALDRKLLESSPKTLKVTANVVVAKDGTGKFKTVNEAVAAAPENSNTR 297
Query: 282 YIIRIKAGVYRENVNVASSKRNIMFWGDGRVNTIITGNRNVVDGSTTFNSATVAAVGERF 341
Y+I +K GVY+E +++ K+N+M GDG+ TIITG+ NV+DGSTTF SATVAA G+ F
Sbjct: 298 YVIYVKKGVYKETIDIGKKKKNLMLVGDGKDATIITGSLNVIDGSTTFRSATVAANGDGF 357
Query: 342 LARDVTFQNTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVQCIIVGTI 401
+A+D+ FQNTAGP+KHQAVALRV +D + RC + AYQDTLY H+ RQFY I GT+
Sbjct: 358 MAQDIWFQNTAGPAKHQAVALRVSADQTVINRCRIDAYQDTLYTHTLRQFYRDSYITGTV 417
Query: 402 DFIFGNAAAVIQDCDIHARRPNPGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVI 461
DFIFGN+A V Q+CDI AR P GQ+NM+TAQGR D NQNT I IQKC+I +SDL PV
Sbjct: 418 DFIFGNSAVVFQNCDIVARNPGAGQKNMLTAQGREDQNQNTAISIQKCKITASSDLAPVK 477
Query: 462 SNFPTFLGRPWQRYSRTVVMQTSISNVIDPAGWHVWDGNFALDTLFYAEYQNSGAGADTS 521
+ TFLGRPW+ YSRTV+MQ+ I N IDPAGW WDG FAL TL+Y EY N+G GADTS
Sbjct: 478 GSVKTFLGRPWKLYSRTVIMQSFIDNHIDPAGWFPWDGEFALSTLYYGEYANTGPGADTS 537
Query: 522 RRVKWKGFRVLTRAAEAEAFTAGNFIGGGTWLSSTGFPF 558
+RV WKGF+V+ + EAE FT I GG WL TG F
Sbjct: 538 KRVNWKGFKVIKDSKEAEQFTVAKLIQGGLWLKPTGVTF 553
BLAST of CSPI03G28630 vs. TAIR 10
Match:
AT5G53370.1 (pectin methylesterase PCR fragment F )
HSP 1 Score: 406.4 bits (1043), Expect = 3.8e-113
Identity = 223/529 (42.16%), Postives = 322/529 (60.87%), Query Frame = 0
Query: 42 VGSKCAMTLYPELCETTISTAVGSSSKEAIEASVNITISAVKDNYKRVQKLLKTV--KNL 101
+ C+ +LYP LC T+ GS + + E ++I+ +A + + T+ +
Sbjct: 75 ISRTCSKSLYPNLCIDTLLDFPGSLTADENEL-IHISFNATLQKFSKALYTSSTITYTQM 134
Query: 102 TKRQKIAFHDCLETGEETLRELYEVVEDVNEYPKKKSLSRYADDLKTLLSSAITNQETCV 161
R + A+ CLE ++++ L + V +S S D+ T LSSA+TN +TC
Sbjct: 135 PPRVRSAYDSCLELLDDSVDALTRALSSVVVVSGDESHS----DVMTWLSSAMTNHDTCT 194
Query: 162 DGFSHDKGD-KKVRESLKEGLIHIEKLCSIALAL----IKNLTDTDIANLNNNNNNNHLN 221
DGF +G +V++ + + + ++ S LA+ +K+L+ + N N
Sbjct: 195 DGFDEIEGQGGEVKDQVIGAVKDLSEMVSNCLAIFAGKVKDLSGVPVVN----------N 254
Query: 222 RKQLEEKKMEDGIKWPDWMSPKDRRLLQASSTA-TPDVVVAADGSGDFRTISEAVAAAPS 281
RK L ++ E+ P+W+ +DR LL ++A D+ V+ DGSG F+TI+EA+ AP
Sbjct: 255 RKLLGTEETEE---LPNWLKREDRELLGTPTSAIQADITVSKDGSGTFKTIAEAIKKAPE 314
Query: 282 RSSRRYIIRIKAGVY-RENVNVASSKRNIMFWGDGRVNTIITGNRNVVDGSTTFNSATVA 341
SSRR++I +KAG Y EN+ V K N+MF GDG+ T+ITG +++ D TTF++AT A
Sbjct: 315 HSSRRFVIYVKAGRYEEENLKVGRKKTNLMFIGDGKGKTVITGGKSIADDLTTFHTATFA 374
Query: 342 AVGERFLARDVTFQNTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVQC 401
A G F+ RD+TF+N AGP+KHQAVALRVG D + YRC+++ YQD LYVHSNRQF+ +C
Sbjct: 375 ATGAGFIVRDMTFENYAGPAKHQAVALRVGGDHAVVYRCNIIGYQDALYVHSNRQFFREC 434
Query: 402 IIVGTIDFIFGNAAAVIQDCDIHARRPNPGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTS 461
I GT+DFIFGNAA ++Q C+I+AR+P Q+ +TAQ R DPNQNTGI I C++ T
Sbjct: 435 EIYGTVDFIFGNAAVILQSCNIYARKPMAQQKITITAQNRKDPNQNTGISIHACKLLATP 494
Query: 462 DLRPVISNFPTFLGRPWQRYSRTVVMQTSISNVIDPAGWHVWDGNFALDTLFYAEYQNSG 521
DL ++PT+LGRPW+ YSR V M + + + IDP GW W+G FALD+L+Y EY N G
Sbjct: 495 DLEASKGSYPTYLGRPWKLYSRVVYMMSDMGDHIDPRGWLEWNGPFALDSLYYGEYMNKG 554
Query: 522 AGADTSRRVKWKGFRVLTRAAEAEAFTAGNFIGGGTWLSSTGFPFSLGL 562
G+ +RVKW G+ V+T EA FT FI G +WL STG F GL
Sbjct: 555 LGSGIGQRVKWPGYHVITSTVEASKFTVAQFISGSSWLPSTGVSFFSGL 585
BLAST of CSPI03G28630 vs. TAIR 10
Match:
AT2G45220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )
HSP 1 Score: 405.2 bits (1040), Expect = 8.5e-113
Identity = 250/547 (45.70%), Postives = 339/547 (61.97%), Query Frame = 0
Query: 22 LLVFALSTTLLLAVVS--SDDHVGSKCAMTLYPELCETTISTAVGSSSKEAIEASVNITI 81
++ F + L+ + VS + V + C+ T P+ CE ++ + ++ + I++
Sbjct: 8 IINFVILCILVASTVSGYNQKDVKAWCSQTPNPKPCEYFLTHNSNNEPIKSESEFLKISM 67
Query: 82 SAVKDN--YKRVQKLLKTVKNLTKRQKIAFHDCLETGEETLRELYEVVEDVNEYPKKKSL 141
V D + K R+K A+ DC++ + T+ ++ E ++ P K
Sbjct: 68 KLVLDRAILAKTHAFTLGPKCRDTREKAAWEDCIKLYDLTVSKINETMD-----PNVKCS 127
Query: 142 SRYADDLKTLLSSAITNQETCVDGFSHDKGDKKVRESLKEGLIHIEKLCSIALALIKNLT 201
D +T LS+A+TN +TC GF L+ G + I L L+ N
Sbjct: 128 KL---DAQTWLSTALTNLDTCRAGF------------LELG------VTDIVLPLMSN-- 187
Query: 202 DTDIANLNNNN---NNNHLNRKQLEEKKMEDGIKWPDWMSPKDRRLLQASSTATPDVVVA 261
+++NL N N N E +DG +P W+ P DR+LLQ SST + VVA
Sbjct: 188 --NVSNLLCNTLAINKVPFNYTPPE----KDG--FPSWVKPGDRKLLQ-SSTPKDNAVVA 247
Query: 262 ADGSGDFRTISEAVAAAPSRSSRRYIIRIKAGVYRENVNVASSKRNIMFWGDGRVNTIIT 321
DGSG+F+TI EA+ AA S R++I +K GVY EN+ + K+N+M GDG TIIT
Sbjct: 248 KDGSGNFKTIKEAIDAA--SGSGRFVIYVKQGVYSENLEI--RKKNVMLRGDGIGKTIIT 307
Query: 322 GNRNVVDGSTTFNSATVAAVGERFLARDVTFQNTAGPSKHQAVALRVGSDLSAFYRCDML 381
G+++V G+TTFNSATVAAVG+ F+AR +TF+NTAG S QAVALR GSDLS FY+C
Sbjct: 308 GSKSVGGGTTTFNSATVAAVGDGFIARGITFRNTAGASNEQAVALRSGSDLSVFYQCSFE 367
Query: 382 AYQDTLYVHSNRQFYVQCIIVGTIDFIFGNAAAVIQDCDIHARRPNPGQRNMVTAQGRTD 441
AYQDTLYVHSNRQFY C + GT+DFIFGNAAAV+Q+C+I ARRP + N +TAQGR+D
Sbjct: 368 AYQDTLYVHSNRQFYRDCDVYGTVDFIFGNAAAVLQNCNIFARRPR-SKTNTITAQGRSD 427
Query: 442 PNQNTGIVIQKCRIGTTSDLRPVISNFPTFLGRPWQRYSRTVVMQTSISNVIDPAGWHVW 501
PNQNTGI+I R+ SDLRPV+ + T+LGRPW++YSRTV M+TS+ ++IDP GW W
Sbjct: 428 PNQNTGIIIHNSRVTAASDLRPVLGSTKTYLGRPWRQYSRTVFMKTSLDSLIDPRGWLEW 487
Query: 502 DGNFALDTLFYAEYQNSGAGADTSRRVKWKGFRVLTRAAEAEAFTAGNFIGGGTWLSSTG 561
DGNFAL TLFYAE+QN+G GA TS RV W GFRVL A+EA FT G F+ GG+W+ S+
Sbjct: 488 DGNFALKTLFYAEFQNTGPGASTSGRVTWPGFRVLGSASEASKFTVGTFLAGGSWIPSS- 511
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| O04886 | 2.7e-204 | 68.64 | Pectinesterase 1 OS=Citrus sinensis OX=2711 GN=PECS-1.1 PE=2 SV=1 | [more] |
| P83948 | 1.0e-203 | 68.45 | Pectinesterase 3 OS=Citrus sinensis OX=2711 PE=1 SV=1 | [more] |
| Q43143 | 1.7e-203 | 62.71 | Pectinesterase/pectinesterase inhibitor U1 OS=Solanum lycopersicum OX=4081 GN=PM... | [more] |
| O49006 | 7.3e-202 | 66.85 | Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PME... | [more] |
| Q42534 | 5.1e-195 | 65.24 | Pectinesterase 2 OS=Arabidopsis thaliana OX=3702 GN=PME2 PE=2 SV=2 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A0A0LCR6 | 0.0e+00 | 99.82 | Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G646640 PE=3 SV=1 | [more] |
| A0A5A7UEV0 | 6.0e-300 | 94.65 | Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold522G00109... | [more] |
| A0A1S3CCA5 | 6.0e-300 | 94.65 | Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103499204 PE=3 SV=1 | [more] |
| A0A6J1ITZ4 | 9.7e-258 | 80.56 | Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111479907 PE=3 SV=1 | [more] |
| A0A6J1GV42 | 5.3e-256 | 80.03 | Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111457458 PE=3 SV=1 | [more] |
