CSPI03G28480 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI03G28480
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	(S)-2-hydroxy-acid oxidase
Location	Chr3: 26069357 .. 26073204 (-)
RNA-Seq Expression	CSPI03G28480
Synteny	CSPI03G28480

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GACTAACTGTTATTTCTTAGATTCTATCTGTCAGCTCTACTTTCTGTTGGTGAACTGCTTTCGTAAGTCAGTTTTTGTGAATATGATTATTTGTAGTCCATTTCATTTTGAGTAATAATATTTGTGGGTCATCTTGCTTTTCTCTGTTTCTGATGCCTTTACAATGTTCATTGCTTCAGTTTTACTTGTCATGAAAATCACCCTCTAAACTAATAATTATCAATTAATATTTGTGACTCTATATGTACTGTTAATTTGTCAAGTTCCACATCGGTAATTTGGTTACCCAATATGCTTGTATTGTGATTTTCAGTCGGGAAACTCTCCGCATCCTCAATAAGTAGGTTAAGATCGTTGCAGAGAAAAAAAAGTTGCAAAATTGCGTAACATATTATTTACAAAGTTTCTCTCTGTCTCTCTCTTCTCAGAAAAAGACTAAAGAACCAAATCCAATGATTGAACTTCTGGGCAAGACCTTCCTATATTCATCAACTGTTTGAACGACATATGCTTTATTAGTCCTCTCGTTAAGTATTTTCTTTGACTTGATTACTGGGTGTTGTGGAGAACTCACTCTTGTCTCCATTAATTGGATGCATTATCCAAAATTGATTGTACCAAAAGCTCTTATTATTAATTTCAAGAAAGTTGAGAAAGTTCTCTTAATTCAATCCACTGTGCTTCACGACCTTCAATGGTTTGTGTGGCATTTGTTACTTCTAAAAAGTTTTAGTATCAATCACTTGAGGATGTACTGGTCTCTATTTTGTCAGGTTAACATGTCTTCAGAACCAGTGAACATAAATGACTTCAAAGAATTGGCGAGGTTGGCACTTCCAAAAATGTACTACGATTTCTACTCCGGGGGAGCTGAGGACGAACACACTCTTAGAGAGAACATACAAGCTTTCTATAGAATCACGTAATTTTGAAGCTAACTAATAATTACTTTGAAAAAGTCATGCTATATGTTATTCATGGTTTCTATGGTTTATAGGATTCGACCTCGTGTTCTCGTAGATGTGAGCAAAATTGATATGTCAACAATCATATTGGGACATCACGTCTCCGCACCTATCCTGGTTGCTCCCACTGCTGCACATAAGTTGGCATTCCATGAAGGTTAAACCCTTTGGATTATAGCTTAGTTGAGAATTTATTTTCAGAATACTTTTTCTTCATCGTATCTGTGTTCTTTCAGGAGAAATAGCCACCGCCAGGGCAGCTGCTGCTGTAAAAACTATAATGGTACTGGACCTAATGTGACTACAAGTTGTAAGTTATTTTACTCGTTCAACTTGGTAACTTAATTAGCTGCTTTCCTTGCAGGTTCTGTCCTACTCCTCGACCTGTTCAATAGAGGAAGTTGCCTCTAGCTGTAATTCTGTCCGTTTCTTCCAATTATATGTAAATGACACTTCTTTTGCACATTTTACTATCCCTCTTTCAGTCCTTTCCTGTTGCATCAATCCAATTGTTTGCAATTTTTTAATAGTTTCTCATTCTGTAATCATTAGATTTTCAAAAGGCGTGATATCTCGGCTCTGCTAGTACAAAGAGCTGAGAGGTTTGGATACAAAGCAATTATCTTGACTGTTGATACACCTCGACTTGGTAGAAGGGAGGCTGACATAAAGAACAAGTATCAATGCTTCATATCTCAGTTTCTTTCCATAGAATATTTTCTATTCCTATCTTTACATACCAATTGTAAGCTATGCCTTAGTTTTTGTCTTATCTGGAATAGCACTTGATCAAATTTGTAGGATGATTGCACCTCCAGTGAAAAGTCTTGAAGGCCTCATTTCTATTGACGTTAAGTCTGTGAGTAATATTTTGATAATCTGGTTTAGAACATTAGAAGTACAACTCACAAACACAATGGCGGAATTTTTTATCTTACTTGATCAGTAGTCTGGTTCCCTTTCCCTCAAGCGTGCTACCATCTACCATGCATCTTAATTCATGTCAAATTTTAAAACCTCATTTCTACTGCTCCTAAGATTATTTACAACTCACAATCATTTATGTAGGATCAAGGTTCAAAATTAGAAACTTATGCCAACGAGATGTTGGATGCATCTCTACGCTGGGAAGTATGTCTGATGAACTGCTCACACTCCATGTATGTTTATTTTATTTCTATATTATTTATAACATTTTAACATTTACAGGATATAGGGTGGTTAAGATCAATCACTACTCTGCCAATTCTAATAAAGGGGGTTCTCACTCATGAAGATGGTAAGTTTAAGTTGTATTTTAAGAATATAAGAATCAAGAAATGAAGCCTCGGATGAGTTATTTAGTGCCCATTGATAGGTGACTTGGTTATTTAACATGTTTGGTGATTGGCAGCAACCAAAGCTGTGGAAGCAGGTGTTGATGGAATTATTGTGTCCAATCATGGAGCCCGCCAGTTAGACTTTGCTCCTGCCACTATTTCTGTCCTTGAAGAGGTAAGTTTCATTATATACTACTTCTATATTAGCAAAATATGCGAAAATATGAAGATAAAATTACTTCAATATCTGCATAAGCATGAACGAATTTCCACGTGACTTCAACTAGATTAGAATGAGAGACAAGCTTTATGAAGTACAGTTAAAAAATATTATCCAACATCTACGTCTTTCCTTTACTAATTAAGTGTAATAATCTTTATCAGTAAGGTTATTTTAACAGGAATTTTGATGCAGGTTGTGCACGCTGTCAAGGGTAAAATCCCTGTGCTCTTAGATGGTGGTGTGCGGCGAGGCACAGATGTGTTCAAGGCCCTAGCCCTTGGTGCTCAGGCAGTCTTGGTAAAATTCCATATCTATTCAACTTTTCTTTTTCTTTAAATTCACGAATGCACTACTAAGCACAAATTTAAAAGTTTAGAAAGTTTAAGGTCAATTATAAAATTCCATAAGTTATTTTTTAAGCAATTTAAATATATCAGTAACCTACTGGAAACAAAGTTTACAGGTGAGAGAGTAAATTGACTTTTTTAAAGTTAAAAGGGACCTGTGATCAATTGGATATTTTTTAAGTTTATAAGTTAAACTTCGTAATTTAATCCACATTTTTGTGTGATCAATTAGATACTTTTTAAGTTCAGAGGTTACGATAGATACAAATTTGAAACTTAAGAGATTAAACTTGTAATTTAACCTACATTTTTCATTCCAAAAACAAAAGGTCTCCAATGTTAAATCTGTGCCAAACAATGTTAGCTGAAATAATGGGGCTGTTGATTTCAACTGAAAATTTAATTATGTTTCTTGTCTCTGAGAAACAACGATCCCTTAAAGACTGGATTGCTTGATTATATGGGGATGGATATTGTTTCTGATGTTAGAGAATTGGTTGTGATTTGATTCAGATAGGGAGACCAGTTTTATATGGGCTAGCAGCAAAGGGAGAGGAGGGAGTAATAACAGTGTTGGAAATGCTGAAAAATGAGTTAGAGACAAGTATGGCACTTTCTGGTTGCCCAAGCATCAAGGACATTACTCGAAGCCATGTGAGGACACACTGTGATAATCTACCATCAATGTTGTAATGGCTTCGCCCTGTATATCATTTAAGTTAATGAG

mRNA sequence

GACTAACTGTTATTTCTTAGATTCTATCTGTCAGCTCTACTTTCTGTTGGTGAACTGCTTTCGTTAACATGTCTTCAGAACCAGTGAACATAAATGACTTCAAAGAATTGGCGAGGTTGGCACTTCCAAAAATGTACTACGATTTCTACTCCGGGGGAGCTGAGGACGAACACACTCTTAGAGAGAACATACAAGCTTTCTATAGAATCACGATTCGACCTCGTGTTCTCGTAGATGTGAGCAAAATTGATATGTCAACAATCATATTGGGACATCACGTCTCCGCACCTATCCTGGTTGCTCCCACTGCTGCACATAAGTTGGCATTCCATGAAGGAGAAATAGCCACCGCCAGGGCAGCTGCTGCTGTAAAAACTATAATGGTTCTGTCCTACTCCTCGACCTGTTCAATAGAGGAAGTTGCCTCTAGCTGTAATTCTGTCCGTTTCTTCCAATTATATATTTTCAAAAGGCGTGATATCTCGGCTCTGCTAGTACAAAGAGCTGAGAGGTTTGGATACAAAGCAATTATCTTGACTGTTGATACACCTCGACTTGGTAGAAGGGAGGCTGACATAAAGAACAAGATGATTGCACCTCCAGTGAAAAGTCTTGAAGGCCTCATTTCTATTGACGTTAAGTCTGATCAAGGTTCAAAATTAGAAACTTATGCCAACGAGATGTTGGATGCATCTCTACGCTGGGAAGATATAGGGTGGTTAAGATCAATCACTACTCTGCCAATTCTAATAAAGGGGGTTCTCACTCATGAAGATGCAACCAAAGCTGTGGAAGCAGGTGTTGATGGAATTATTGTGTCCAATCATGGAGCCCGCCAGTTAGACTTTGCTCCTGCCACTATTTCTGTCCTTGAAGAGGTTGTGCACGCTGTCAAGGGTAAAATCCCTGTGCTCTTAGATGGTGGTGTGCGGCGAGGCACAGATGTGTTCAAGGCCCTAGCCCTTGGTGCTCAGGCAGTCTTGATAGGGAGACCAGTTTTATATGGGCTAGCAGCAAAGGGAGAGGAGGGAGTAATAACAGTGTTGGAAATGCTGAAAAATGAGTTAGAGACAAGTATGGCACTTTCTGGTTGCCCAAGCATCAAGGACATTACTCGAAGCCATGTGAGGACACACTGTGATAATCTACCATCAATGTTGTAATGGCTTCGCCCTGTATATCATTTAAGTTAATGAG

Coding sequence (CDS)

ATGTCTTCAGAACCAGTGAACATAAATGACTTCAAAGAATTGGCGAGGTTGGCACTTCCAAAAATGTACTACGATTTCTACTCCGGGGGAGCTGAGGACGAACACACTCTTAGAGAGAACATACAAGCTTTCTATAGAATCACGATTCGACCTCGTGTTCTCGTAGATGTGAGCAAAATTGATATGTCAACAATCATATTGGGACATCACGTCTCCGCACCTATCCTGGTTGCTCCCACTGCTGCACATAAGTTGGCATTCCATGAAGGAGAAATAGCCACCGCCAGGGCAGCTGCTGCTGTAAAAACTATAATGGTTCTGTCCTACTCCTCGACCTGTTCAATAGAGGAAGTTGCCTCTAGCTGTAATTCTGTCCGTTTCTTCCAATTATATATTTTCAAAAGGCGTGATATCTCGGCTCTGCTAGTACAAAGAGCTGAGAGGTTTGGATACAAAGCAATTATCTTGACTGTTGATACACCTCGACTTGGTAGAAGGGAGGCTGACATAAAGAACAAGATGATTGCACCTCCAGTGAAAAGTCTTGAAGGCCTCATTTCTATTGACGTTAAGTCTGATCAAGGTTCAAAATTAGAAACTTATGCCAACGAGATGTTGGATGCATCTCTACGCTGGGAAGATATAGGGTGGTTAAGATCAATCACTACTCTGCCAATTCTAATAAAGGGGGTTCTCACTCATGAAGATGCAACCAAAGCTGTGGAAGCAGGTGTTGATGGAATTATTGTGTCCAATCATGGAGCCCGCCAGTTAGACTTTGCTCCTGCCACTATTTCTGTCCTTGAAGAGGTTGTGCACGCTGTCAAGGGTAAAATCCCTGTGCTCTTAGATGGTGGTGTGCGGCGAGGCACAGATGTGTTCAAGGCCCTAGCCCTTGGTGCTCAGGCAGTCTTGATAGGGAGACCAGTTTTATATGGGCTAGCAGCAAAGGGAGAGGAGGGAGTAATAACAGTGTTGGAAATGCTGAAAAATGAGTTAGAGACAAGTATGGCACTTTCTGGTTGCCCAAGCATCAAGGACATTACTCGAAGCCATGTGAGGACACACTGTGATAATCTACCATCAATGTTGTAA

Protein sequence

MSSEPVNINDFKELARLALPKMYYDFYSGGAEDEHTLRENIQAFYRITIRPRVLVDVSKIDMSTIILGHHVSAPILVAPTAAHKLAFHEGEIATARAAAAVKTIMVLSYSSTCSIEEVASSCNSVRFFQLYIFKRRDISALLVQRAERFGYKAIILTVDTPRLGRREADIKNKMIAPPVKSLEGLISIDVKSDQGSKLETYANEMLDASLRWEDIGWLRSITTLPILIKGVLTHEDATKAVEAGVDGIIVSNHGARQLDFAPATISVLEEVVHAVKGKIPVLLDGGVRRGTDVFKALALGAQAVLIGRPVLYGLAAKGEEGVITVLEMLKNELETSMALSGCPSIKDITRSHVRTHCDNLPSML*

Homology

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BLAST of CSPI03G28480 vs. ExPASy Swiss-Prot
Match: Q9LJH5 (Peroxisomal (S)-2-hydroxy-acid oxidase GLO4 OS=Arabidopsis thaliana OX=3702 GN=GLO4 PE=2 SV=1)

HSP 1 Score: 530.0 bits (1364), Expect = 2.1e-149
Identity = 258/359 (71.87%), Postives = 318/359 (88.58%), Query Frame = 0


Query: 6   VNINDFKELARLALPKMYYDFYSGGAEDEHTLRENIQAFYRITIRPRVLVDVSKIDMSTI 65
           VN+++F+ELA+ ALPKMYYDFY+GGAED+HTL EN+QAF RI  RPRVLVDVS IDMST 
Sbjct: 5   VNVDEFQELAKQALPKMYYDFYNGGAEDQHTLNENVQAFRRIMFRPRVLVDVSNIDMSTS 64

Query: 66  ILGHHVSAPILVAPTAAHKLAFHEGEIATARAAAAVKTIMVLSYSSTCSIEEVASSCNSV 125
           +LG+ +SAPI++APTA HKLA  +GEIATA+AAAA  TIM++S+ STC+IEEVASSCN+V
Sbjct: 65  MLGYPISAPIMIAPTAMHKLAHPKGEIATAKAAAACNTIMIVSFMSTCTIEEVASSCNAV 124

Query: 126 RFFQLYIFKRRDISALLVQRAERFGYKAIILTVDTPRLGRREADIKNKMIAPPVKSLEGL 185
           RF Q+Y++KRRD++A +V+RAE+ G+KAI+LTVD PRLGRREADIKNKMI+P +K+ EGL
Sbjct: 125 RFLQIYVYKRRDVTAQIVKRAEKAGFKAIVLTVDVPRLGRREADIKNKMISPQLKNFEGL 184

Query: 186 ISIDVKSDQGSKLETYANEMLDASLRWEDIGWLRSITTLPILIKGVLTHEDATKAVEAGV 245
           +S +V+ ++GS +E +A+   DASL W+DI WLRSIT LPIL+KG+LT EDA KAVEAGV
Sbjct: 185 VSTEVRPNEGSGVEAFASSAFDASLSWKDIEWLRSITKLPILVKGLLTREDALKAVEAGV 244

Query: 246 DGIIVSNHGARQLDFAPATISVLEEVVHAVKGKIPVLLDGGVRRGTDVFKALALGAQAVL 305
           DGI+VSNHGARQLD++PATI+VLEEVVHAVKG+IPVLLDGGVRRGTDVFKALALGAQAVL
Sbjct: 245 DGIVVSNHGARQLDYSPATITVLEEVVHAVKGRIPVLLDGGVRRGTDVFKALALGAQAVL 304

Query: 306 IGRPVLYGLAAKGEEGVITVLEMLKNELETSMALSGCPSIKDITRSHVRTHCDNLPSML 365
           IGRP++YGLAAKGE+GV  V++MLKNE E +MALSGCP+I D+TR+HVRT  + + SML
Sbjct: 305 IGRPIVYGLAAKGEDGVKKVIDMLKNEFEITMALSGCPTIDDVTRNHVRTENERIKSML 363

[Close]

BLAST of CSPI03G28480 vs. ExPASy Swiss-Prot
Match: Q24JJ8 (Peroxisomal (S)-2-hydroxy-acid oxidase GLO3 OS=Arabidopsis thaliana OX=3702 GN=GLO3 PE=2 SV=1)

HSP 1 Score: 518.8 bits (1335), Expect = 4.8e-146
Identity = 255/359 (71.03%), Postives = 310/359 (86.35%), Query Frame = 0


Query: 6   VNINDFKELARLALPKMYYDFYSGGAEDEHTLRENIQAFYRITIRPRVLVDVSKIDMSTI 65
           VN+++F+ELA+ ALPKMYYDFY+GGAED+HTL EN+QAF RI  RPRVLVDVSKIDMST 
Sbjct: 5   VNVDEFQELAKQALPKMYYDFYNGGAEDQHTLNENVQAFRRIMFRPRVLVDVSKIDMSTK 64

Query: 66  ILGHHVSAPILVAPTAAHKLAFHEGEIATARAAAAVKTIMVLSYSSTCSIEEVASSCNSV 125
           ILG+ +SAPI++APT  HKLA  EGE ATA+AAAA  TIM++SY S+C+ EE+ASSCN+V
Sbjct: 65  ILGYPISAPIMIAPTGNHKLAHPEGETATAKAAAACNTIMIVSYMSSCTFEEIASSCNAV 124

Query: 126 RFFQLYIFKRRDISALLVQRAERFGYKAIILTVDTPRLGRREADIKNKMIAPPVKSLEGL 185
           RF Q+Y++KRRDI+A +V+RAE+ G+KAI+LTVD PRLGRREADIKNKMI+P +K+ EGL
Sbjct: 125 RFLQIYVYKRRDITAQVVKRAEKAGFKAIVLTVDVPRLGRREADIKNKMISPQLKNFEGL 184

Query: 186 ISIDVKSDQGSKLETYANEMLDASLRWEDIGWLRSITTLPILIKGVLTHEDATKAVEAGV 245
            S +V+  +GS ++ +A+   DAS  W+DI WLRSIT LPIL+KG+LT EDA KAVEAGV
Sbjct: 185 FSTEVRPSKGSGVQAFASRAFDASFSWKDIEWLRSITELPILVKGILTREDALKAVEAGV 244

Query: 246 DGIIVSNHGARQLDFAPATISVLEEVVHAVKGKIPVLLDGGVRRGTDVFKALALGAQAVL 305
           DGIIVSNHG RQLD++PATI+VLEEVV  V+G+IPVLLDGGVRRGTDVFKALALGAQAVL
Sbjct: 245 DGIIVSNHGGRQLDYSPATITVLEEVVQVVRGRIPVLLDGGVRRGTDVFKALALGAQAVL 304

Query: 306 IGRPVLYGLAAKGEEGVITVLEMLKNELETSMALSGCPSIKDITRSHVRTHCDNLPSML 365
           IGRP++YGLAAKGE+GV  V++MLKNE E +MALSGCP+I DITR+HVRT  + L SML
Sbjct: 305 IGRPIIYGLAAKGEDGVKKVIDMLKNEFEITMALSGCPTIDDITRNHVRTENERLHSML 363

[Close]

BLAST of CSPI03G28480 vs. ExPASy Swiss-Prot
Match: Q8H3I4 (Peroxisomal (S)-2-hydroxy-acid oxidase GLO4 OS=Oryza sativa subsp. japonica OX=39947 GN=GLO4 PE=1 SV=2)

HSP 1 Score: 468.4 bits (1204), Expect = 7.5e-131
Identity = 234/361 (64.82%), Postives = 296/361 (81.99%), Query Frame = 0


Query: 5   PVNINDFKELARLALPKMYYDFYSGGAEDEHTLRENIQAFYRITIRPRVLVDVSKIDMST 64
           PVN+ +++ELA+ ALPKM YD+ +GGAEDEHTLRENI A+ RI +RPRVLVDVSKIDMST
Sbjct: 6   PVNVREYQELAKKALPKMAYDYINGGAEDEHTLRENIAAYTRIILRPRVLVDVSKIDMST 65

Query: 65  IILGHHVSAPILVAPTAAHKLAFHEGEIATARAAAAVKTIMVLSYSSTCSIEEVASSCNS 124
            +LG+ + +PI+VAPT  HKLA  EGE ATARAAA+   IMVLS+SS+C IE+VASSCN+
Sbjct: 66  TLLGYTMRSPIIVAPTGGHKLAHPEGEKATARAAASCNAIMVLSFSSSCKIEDVASSCNA 125

Query: 125 VRFFQLYIFKRRDISALLVQRAERFGYKAIILTVDTPRLGRREADIKNKMIAPPVKSLEG 184
           +RF+QLY++K R++SA LV+RAE  G+KA++LTVDTP LGRREADI+NKM+ P   +LEG
Sbjct: 126 IRFYQLYVYKNRNVSATLVRRAESCGFKALLLTVDTPMLGRREADIRNKMVFPRSGNLEG 185

Query: 185 LISI-DVKSDQGSKLETYANEMLDASLRWEDIGWLRSITTLPILIKGVLTHEDATKAVEA 244
           L++  D  +  GS+LE +A   LD SL W+DI WL+SIT++PI +KG++T EDA +AVEA
Sbjct: 186 LMTTDDHDTTNGSQLERFARATLDPSLSWKDIEWLKSITSMPIFLKGIVTAEDARRAVEA 245

Query: 245 GVDGIIVSNHGARQLDFAPATISVLEEVVHAVKGKIPVLLDGGVRRGTDVFKALALGAQA 304
           GV G+IVSNHGARQLD+APATI+ LEEVV AV G +PVL+DGG+RRGTDVFKALALGA+A
Sbjct: 246 GVAGVIVSNHGARQLDYAPATIAALEEVVRAVAGAVPVLVDGGIRRGTDVFKALALGARA 305

Query: 305 VLIGRPVLYGLAAKGEEGVITVLEMLKNELETSMALSGCPSIKDITRSHVRTHCDNLPSM 364
           V++GRPV +GLAA+GE G   V+EML  ELE +MAL GC S+ +ITRSHV T  D + S+
Sbjct: 306 VMVGRPVFFGLAARGEAGARHVIEMLNGELEVAMALCGCRSVGEITRSHVMTEGDRIRSL 365

[Close]

BLAST of CSPI03G28480 vs. ExPASy Swiss-Prot
Match: B8B8K5 (Peroxisomal (S)-2-hydroxy-acid oxidase GLO4 OS=Oryza sativa subsp. indica OX=39946 GN=GLO4 PE=3 SV=2)

HSP 1 Score: 463.8 bits (1192), Expect = 1.8e-129
Identity = 233/361 (64.54%), Postives = 294/361 (81.44%), Query Frame = 0


Query: 5   PVNINDFKELARLALPKMYYDFYSGGAEDEHTLRENIQAFYRITIRPRVLVDVSKIDMST 64
           PVN+ +++ELA+ ALPKM YD+ +GGAEDEHTLRENI A+ RI +RPRVLVDVSKIDMST
Sbjct: 6   PVNVREYQELAKKALPKMAYDYINGGAEDEHTLRENIAAYTRIILRPRVLVDVSKIDMST 65

Query: 65  IILGHHVSAPILVAPTAAHKLAFHEGEIATARAAAAVKTIMVLSYSSTCSIEEVASSCNS 124
            +LG+ + +PI+VAPT  HKLA  EGE ATARAAA+   IMVLS+SS+C IE+VASSCN+
Sbjct: 66  TLLGYTMRSPIIVAPTGGHKLAHPEGEKATARAAASCNAIMVLSFSSSCKIEDVASSCNA 125

Query: 125 VRFFQLYIFKRRDISALLVQRAERFGYKAIILTVDTPRLGRREADIKNKMIAPPVKSLEG 184
           +RF+QLY++K R++SA LV+RAE  G+KA++LTVDTP LGRREADI+NKM+ P   +LEG
Sbjct: 126 IRFYQLYVYKNRNVSATLVRRAESCGFKALLLTVDTPMLGRREADIRNKMVFPRSGNLEG 185

Query: 185 LISI-DVKSDQGSKLETYANEMLDASLRWEDIGWLRSITTLPILIKGVLTHEDATKAVEA 244
           L++I D  +  GS+LE +A   LD SL W+DI WL+SIT++PI +KG++T EDA +AVEA
Sbjct: 186 LMTIDDHDTTNGSQLERFARATLDPSLSWKDIEWLKSITSMPIFLKGIVTAEDARRAVEA 245

Query: 245 GVDGIIVSNHGARQLDFAPATISVLEEVVHAVKGKIPVLLDGGVRRGTDVFKALALGAQA 304
           GV G+IVSNHGARQLD+APATI+ LEEVV AV G +PVL+DGG+RRGTDVFKALALGA+A
Sbjct: 246 GVAGVIVSNHGARQLDYAPATIAALEEVVRAVAGAVPVLVDGGIRRGTDVFKALALGARA 305

Query: 305 VLIGRPVLYGLAAKGEEGVITVLEMLKNELETSMALSGCPSIKDITRSHVRTHCDNLPSM 364
           V+   PV +GLAA+GE G   V+EML  ELE +MAL GC S+ +ITRSHV T  D + S+
Sbjct: 306 VMXXXPVFFGLAARGEAGARHVIEMLNGELEVAMALCGCRSVGEITRSHVMTEGDRIRSL 365

[Close]

BLAST of CSPI03G28480 vs. ExPASy Swiss-Prot
Match: B8B7C5 (Peroxisomal (S)-2-hydroxy-acid oxidase GLO5 OS=Oryza sativa subsp. indica OX=39946 GN=GLO5 PE=3 SV=1)

HSP 1 Score: 415.6 bits (1067), Expect = 5.7e-115
Identity = 218/365 (59.73%), Postives = 274/365 (75.07%), Query Frame = 0


Query: 4   EPVNINDFKELARLALPKMYYDFYSGGAEDEHTLRENIQAFYRITIRPRVLVDVSKIDMS 63
           E  N+ +++ +A+  LPKM YD+Y+ GAEDE TL+EN +AF RI  RPR+L+DVSKIDM+
Sbjct: 3   EITNVTEYQAIAKQKLPKMIYDYYASGAEDEWTLQENREAFARILFRPRILIDVSKIDMA 62

Query: 64  TIILGHHVSAPILVAPTAAHKLAFHEGEIATARAAAAVKTIMVLSYSSTCSIEEVASSCN 123
           T +LG  +S PI++AP+A  K+A  +GE ATARAA+A  TIM LS  +T S+EEVAS+  
Sbjct: 63  TTVLGFKISMPIMIAPSAMQKMAHPDGEYATARAASAAGTIMTLSSWATSSVEEVASTGP 122

Query: 124 SVRFFQLYIFKRRDISALLVQRAERFGYKAIILTVDTPRLGRREADIKNKMIAPP---VK 183
            +RFFQLY++K R +   LV+RAER G+KAI LTVDTPRLGRREADIKN+ + PP   +K
Sbjct: 123 GIRFFQLYVYKDRRVVEQLVRRAERAGFKAIALTVDTPRLGRREADIKNRFVLPPFLTLK 182

Query: 184 SLEGLI--SIDVKSDQGSKLETYANEMLDASLRWEDIGWLRSITTLPILIKGVLTHEDAT 243
           + EGL    +D  SD G  L +Y    +D +L W+D+ WL++ITTLPIL+KGV+T ED  
Sbjct: 183 NFEGLELGKMDQASDSG--LASYVAGQIDRTLSWKDVKWLQTITTLPILVKGVITAEDTR 242

Query: 244 KAVEAGVDGIIVSNHGARQLDFAPATISVLEEVVHAVKGKIPVLLDGGVRRGTDVFKALA 303
            AVE G  GIIVSNHGARQLD+ PATIS LEEVV A +G++PV LDGGVRRGTDVFKALA
Sbjct: 243 LAVENGAAGIIVSNHGARQLDYVPATISALEEVVKAARGQLPVFLDGGVRRGTDVFKALA 302

Query: 304 LGAQAVLIGRPVLYGLAAKGEEGVITVLEMLKNELETSMALSGCPSIKDITRSHVRTHCD 363
           LGA  V IGRPV++ LAA GE GV  VL+ML++E E +MALSGC S+ DITR+HV T  D
Sbjct: 303 LGAAGVFIGRPVVFSLAAAGEAGVRNVLQMLRDEFELTMALSGCTSLADITRNHVITEAD 362

[Close]

BLAST of CSPI03G28480 vs. ExPASy TrEMBL
Match: A0A0A0LEF4 ((S)-2-hydroxy-acid oxidase OS=Cucumis sativus OX=3659 GN=Csa_3G645990 PE=3 SV=1)

HSP 1 Score: 694.5 bits (1791), Expect = 2.4e-196
Identity = 359/364 (98.63%), Postives = 361/364 (99.18%), Query Frame = 0


Query: 1   MSSEPVNINDFKELARLALPKMYYDFYSGGAEDEHTLRENIQAFYRITIRPRVLVDVSKI 60
           MSSEPVNINDFKELARLALPKMYYDFYSGGAEDEHTLRENIQAFYRITIRPRVL+DVSKI
Sbjct: 1   MSSEPVNINDFKELARLALPKMYYDFYSGGAEDEHTLRENIQAFYRITIRPRVLIDVSKI 60

Query: 61  DMSTIILGHHVSAPILVAPTAAHKLAFHEGEIATARAAAAVKTIMVLSYSSTCSIEEVAS 120
           DMST ILGHHVSAPILVAPTAAHKLAFHEGEIATARAAAAVKTIMVLSYSSTCSIEE+AS
Sbjct: 61  DMSTTILGHHVSAPILVAPTAAHKLAFHEGEIATARAAAAVKTIMVLSYSSTCSIEEIAS 120

Query: 121 SCNSVRFFQLYIFKRRDISALLVQRAERFGYKAIILTVDTPRLGRREADIKNKMIAPPVK 180
           SCNSVRFFQLYIFKRRDISALLVQRAERFGYKAIILTVDTPRLGRREADIKNKMIAPPVK
Sbjct: 121 SCNSVRFFQLYIFKRRDISALLVQRAERFGYKAIILTVDTPRLGRREADIKNKMIAPPVK 180

Query: 181 SLEGLISIDVKSDQGSKLETYANEMLDASLRWEDIGWLRSITTLPILIKGVLTHEDATKA 240
           SLEGLISIDVKSDQGSKLETYANEMLDASLRWEDIGWLRSITTLPILIKGVLTHEDATKA
Sbjct: 181 SLEGLISIDVKSDQGSKLETYANEMLDASLRWEDIGWLRSITTLPILIKGVLTHEDATKA 240

Query: 241 VEAGVDGIIVSNHGARQLDFAPATISVLEEVVHAVKGKIPVLLDGGVRRGTDVFKALALG 300
           VEAGVDGIIVSNHGARQLDFAPATISVLEEVVHAVKGKIPVLLDGGVRRGTDVFKALALG
Sbjct: 241 VEAGVDGIIVSNHGARQLDFAPATISVLEEVVHAVKGKIPVLLDGGVRRGTDVFKALALG 300

Query: 301 AQAVLIGRPVLYGLAAKGEEGVITVLEMLKNELETSMALSGCPSIKDITRSHVRTHCDNL 360
           AQAVLIGRPVLYGLAAKGEEGV TVLEMLKNELETSMALSGCPSIKDITRSHVRTH DNL
Sbjct: 301 AQAVLIGRPVLYGLAAKGEEGVRTVLEMLKNELETSMALSGCPSIKDITRSHVRTHYDNL 360

Query: 361 PSML 365
           PSML
Sbjct: 361 PSML 364

[Close]

BLAST of CSPI03G28480 vs. ExPASy TrEMBL
Match: A0A1S3CCP5 ((S)-2-hydroxy-acid oxidase OS=Cucumis melo OX=3656 GN=LOC103499183 PE=3 SV=1)

HSP 1 Score: 686.0 bits (1769), Expect = 8.4e-194
Identity = 352/364 (96.70%), Postives = 359/364 (98.63%), Query Frame = 0


Query: 1   MSSEPVNINDFKELARLALPKMYYDFYSGGAEDEHTLRENIQAFYRITIRPRVLVDVSKI 60
           MSSEPVN+NDFKELARLALPKMYYDFYSGGAEDEHTLRENIQAFYRITIRPRVLVDVSK+
Sbjct: 1   MSSEPVNVNDFKELARLALPKMYYDFYSGGAEDEHTLRENIQAFYRITIRPRVLVDVSKV 60

Query: 61  DMSTIILGHHVSAPILVAPTAAHKLAFHEGEIATARAAAAVKTIMVLSYSSTCSIEEVAS 120
           DMST ILGHH+SAPILVAPTAAHKLAFHEGE+ATARAAAAVKTIMVLSYSSTCSIEEVAS
Sbjct: 61  DMSTTILGHHISAPILVAPTAAHKLAFHEGELATARAAAAVKTIMVLSYSSTCSIEEVAS 120

Query: 121 SCNSVRFFQLYIFKRRDISALLVQRAERFGYKAIILTVDTPRLGRREADIKNKMIAPPVK 180
           SCNSVRFFQLYIFKRRDISALLVQRAER GYKAIILTVDTPRLGRREADIKNKMIAPPVK
Sbjct: 121 SCNSVRFFQLYIFKRRDISALLVQRAERCGYKAIILTVDTPRLGRREADIKNKMIAPPVK 180

Query: 181 SLEGLISIDVKSDQGSKLETYANEMLDASLRWEDIGWLRSITTLPILIKGVLTHEDATKA 240
           SLEGLISIDVKSDQGSKLETYANEMLDASL WEDIGWLRSITTLPILIKGVLTHEDATKA
Sbjct: 181 SLEGLISIDVKSDQGSKLETYANEMLDASLSWEDIGWLRSITTLPILIKGVLTHEDATKA 240

Query: 241 VEAGVDGIIVSNHGARQLDFAPATISVLEEVVHAVKGKIPVLLDGGVRRGTDVFKALALG 300
           VEAGVDGIIVSNHGARQLDFAPAT+SVLEEVVHAVKGKIPVLLDGGVRRGTDVFKALALG
Sbjct: 241 VEAGVDGIIVSNHGARQLDFAPATVSVLEEVVHAVKGKIPVLLDGGVRRGTDVFKALALG 300

Query: 301 AQAVLIGRPVLYGLAAKGEEGVITVLEMLKNELETSMALSGCPSIKDITRSHVRTHCDNL 360
           AQAVLIGRPV+YGLAAKGEEGV TV+EMLKNELETSMALSGCPSIKDITRSHVRTH DNL
Sbjct: 301 AQAVLIGRPVIYGLAAKGEEGVRTVMEMLKNELETSMALSGCPSIKDITRSHVRTHYDNL 360

Query: 361 PSML 365
           PSML
Sbjct: 361 PSML 364

[Close]

BLAST of CSPI03G28480 vs. ExPASy TrEMBL
Match: A0A5D3D6C7 ((S)-2-hydroxy-acid oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold522G00360 PE=3 SV=1)

HSP 1 Score: 671.8 bits (1732), Expect = 1.6e-189
Identity = 352/390 (90.26%), Postives = 359/390 (92.05%), Query Frame = 0


Query: 1   MSSEPVNINDFKELARLALPKMYYDFYSGGAEDEHTLRENIQAFYRITIRPRVLVDVSKI 60
           MSSEPVN+NDFKELARLALPKMYYDFYSGGAEDEHTLRENIQAFYRITIRPRVLVDVSK+
Sbjct: 1   MSSEPVNVNDFKELARLALPKMYYDFYSGGAEDEHTLRENIQAFYRITIRPRVLVDVSKV 60

Query: 61  DMSTIILGHHVSAPILVAPTAAHKLAFHEGEIATARAAAAVKTIMVLSYSSTCSIEEVAS 120
           DMST ILGHH+SAPILVAPTAAHKLAFHEGE+ATARAAAAVKTIMVLSYSSTCSIEEVAS
Sbjct: 61  DMSTTILGHHISAPILVAPTAAHKLAFHEGELATARAAAAVKTIMVLSYSSTCSIEEVAS 120

Query: 121 SCNSVRFFQLYIFKRRDISALLVQRAERFGYKAIILTVDTPRLGRREADIKNKMIAPPVK 180
           SCNSVRFFQLYIFKRRDISALLVQRAER GYKAIILTVDTPRLGRREADIKNKMIAPPVK
Sbjct: 121 SCNSVRFFQLYIFKRRDISALLVQRAERCGYKAIILTVDTPRLGRREADIKNKMIAPPVK 180

Query: 181 SLEGLISIDVKSDQGSKLETYANEMLDASLRWE--------------------------D 240
           SLEGLISIDVKSDQGSKLETYANEMLDASL WE                          D
Sbjct: 181 SLEGLISIDVKSDQGSKLETYANEMLDASLSWEVCLMNCSHSMYVYFISGLVITFYHLQD 240

Query: 241 IGWLRSITTLPILIKGVLTHEDATKAVEAGVDGIIVSNHGARQLDFAPATISVLEEVVHA 300
           IGWLRSITTLPILIKGVLTHEDATKAVEAGVDGIIVSNHGARQLDFAPAT+SVLEEVVHA
Sbjct: 241 IGWLRSITTLPILIKGVLTHEDATKAVEAGVDGIIVSNHGARQLDFAPATVSVLEEVVHA 300

Query: 301 VKGKIPVLLDGGVRRGTDVFKALALGAQAVLIGRPVLYGLAAKGEEGVITVLEMLKNELE 360
           VKGKIPVLLDGGVRRGTDVFKALALGAQAVLIGRPV+YGLAAKGEEGV TV+EMLKNELE
Sbjct: 301 VKGKIPVLLDGGVRRGTDVFKALALGAQAVLIGRPVIYGLAAKGEEGVRTVMEMLKNELE 360

Query: 361 TSMALSGCPSIKDITRSHVRTHCDNLPSML 365
           TSMALSGCPSIKDITRSHVRTH DNLPSML
Sbjct: 361 TSMALSGCPSIKDITRSHVRTHYDNLPSML 390

[Close]

BLAST of CSPI03G28480 vs. ExPASy TrEMBL
Match: A0A5A7UCM3 ((S)-2-hydroxy-acid oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold190G00820 PE=3 SV=1)

HSP 1 Score: 671.4 bits (1731), Expect = 2.1e-189
Identity = 351/390 (90.00%), Postives = 359/390 (92.05%), Query Frame = 0


Query: 1   MSSEPVNINDFKELARLALPKMYYDFYSGGAEDEHTLRENIQAFYRITIRPRVLVDVSKI 60
           MSSEPVN+NDFKELARLALPKMYYDFYSGGAEDEHTLRENIQAFYRITIRPRVLVDVSK+
Sbjct: 1   MSSEPVNVNDFKELARLALPKMYYDFYSGGAEDEHTLRENIQAFYRITIRPRVLVDVSKV 60

Query: 61  DMSTIILGHHVSAPILVAPTAAHKLAFHEGEIATARAAAAVKTIMVLSYSSTCSIEEVAS 120
           DMST ILGHH+SAPILVAPTAAHKLAFHEGE+ATARAAAAVKTIMVLSYSSTCSIEEVAS
Sbjct: 61  DMSTTILGHHISAPILVAPTAAHKLAFHEGELATARAAAAVKTIMVLSYSSTCSIEEVAS 120

Query: 121 SCNSVRFFQLYIFKRRDISALLVQRAERFGYKAIILTVDTPRLGRREADIKNKMIAPPVK 180
           SCNSVRFFQLYIFKRRDISALLVQRAER GYKAIILTVDTPRLGRREADIKNKMIAPPVK
Sbjct: 121 SCNSVRFFQLYIFKRRDISALLVQRAERCGYKAIILTVDTPRLGRREADIKNKMIAPPVK 180

Query: 181 SLEGLISIDVKSDQGSKLETYANEMLDASLRWE--------------------------D 240
           SLEGLISIDVKSDQGSKLETYANEMLDASL WE                          D
Sbjct: 181 SLEGLISIDVKSDQGSKLETYANEMLDASLSWEVCLMNCSHSMYVYFISGLVITFYHLQD 240

Query: 241 IGWLRSITTLPILIKGVLTHEDATKAVEAGVDGIIVSNHGARQLDFAPATISVLEEVVHA 300
           IGWLRSITTLPILIKG+LTHEDATKAVEAGVDGIIVSNHGARQLDFAPAT+SVLEEVVHA
Sbjct: 241 IGWLRSITTLPILIKGILTHEDATKAVEAGVDGIIVSNHGARQLDFAPATVSVLEEVVHA 300

Query: 301 VKGKIPVLLDGGVRRGTDVFKALALGAQAVLIGRPVLYGLAAKGEEGVITVLEMLKNELE 360
           VKGKIPVLLDGGVRRGTDVFKALALGAQAVLIGRPV+YGLAAKGEEGV TV+EMLKNELE
Sbjct: 301 VKGKIPVLLDGGVRRGTDVFKALALGAQAVLIGRPVIYGLAAKGEEGVRTVMEMLKNELE 360

Query: 361 TSMALSGCPSIKDITRSHVRTHCDNLPSML 365
           TSMALSGCPSIKDITRSHVRTH DNLPSML
Sbjct: 361 TSMALSGCPSIKDITRSHVRTHYDNLPSML 390

[Close]

BLAST of CSPI03G28480 vs. ExPASy TrEMBL
Match: A0A6J1GVS5 ((S)-2-hydroxy-acid oxidase OS=Cucurbita moschata OX=3662 GN=LOC111457615 PE=3 SV=1)

HSP 1 Score: 649.8 bits (1675), Expect = 6.7e-183
Identity = 335/364 (92.03%), Postives = 347/364 (95.33%), Query Frame = 0


Query: 1   MSSEPVNINDFKELARLALPKMYYDFYSGGAEDEHTLRENIQAFYRITIRPRVLVDVSKI 60
           MSSEPVN+NDFKELARL LPKMYYDFY+GGAEDEHTLRENIQAF RI I+PRVLVDVS+I
Sbjct: 1   MSSEPVNVNDFKELARLKLPKMYYDFYAGGAEDEHTLRENIQAFCRIMIQPRVLVDVSQI 60

Query: 61  DMSTIILGHHVSAPILVAPTAAHKLAFHEGEIATARAAAAVKTIMVLSYSSTCSIEEVAS 120
           DMST ILGH +SAPILVAPTAAHKLAFHEGE ATARAAAA KTIM+LSYSSTCSIEEVAS
Sbjct: 61  DMSTTILGHRISAPILVAPTAAHKLAFHEGEQATARAAAAAKTIMILSYSSTCSIEEVAS 120

Query: 121 SCNSVRFFQLYIFKRRDISALLVQRAERFGYKAIILTVDTPRLGRREADIKNKMIAPPVK 180
           SCN+VRFFQLYIFKRRDISALLVQRAERFGYKAI+LT DTPRLGRREADIKNKMIAPPVK
Sbjct: 121 SCNAVRFFQLYIFKRRDISALLVQRAERFGYKAIVLTADTPRLGRREADIKNKMIAPPVK 180

Query: 181 SLEGLISIDVKSDQGSKLETYANEMLDASLRWEDIGWLRSITTLPILIKGVLTHEDATKA 240
           +LEGLISIDV SDQGSKLETYANEMLDASLRWEDI WLRSIT+LPILIKG+LTHEDATKA
Sbjct: 181 NLEGLISIDVNSDQGSKLETYANEMLDASLRWEDISWLRSITSLPILIKGILTHEDATKA 240

Query: 241 VEAGVDGIIVSNHGARQLDFAPATISVLEEVVHAVKGKIPVLLDGGVRRGTDVFKALALG 300
           VEAGVDGIIVSNHGARQLDFAPATISVLEEVVHAVKGKIPVLLDGGVRRGTDVFKALALG
Sbjct: 241 VEAGVDGIIVSNHGARQLDFAPATISVLEEVVHAVKGKIPVLLDGGVRRGTDVFKALALG 300

Query: 301 AQAVLIGRPVLYGLAAKGEEGVITVLEMLKNELETSMALSGCPSIKDITRSHVRTHCDNL 360
           AQAVLIGRPVL+GLAAKGE GV TVLEMLKNELE SMALSGCP IKDITRSHVRT  D L
Sbjct: 301 AQAVLIGRPVLFGLAAKGEGGVRTVLEMLKNELEISMALSGCPCIKDITRSHVRTPYDKL 360

Query: 361 PSML 365
           PSML
Sbjct: 361 PSML 364

[Close]

BLAST of CSPI03G28480 vs. NCBI nr
Match: XP_004144438.2 (peroxisomal (S)-2-hydroxy-acid oxidase GLO4 [Cucumis sativus] >XP_031738757.1 peroxisomal (S)-2-hydroxy-acid oxidase GLO4 [Cucumis sativus] >XP_031738758.1 peroxisomal (S)-2-hydroxy-acid oxidase GLO4 [Cucumis sativus] >KGN58624.2 hypothetical protein Csa_002146 [Cucumis sativus])

HSP 1 Score: 697.2 bits (1798), Expect = 7.5e-197
Identity = 360/364 (98.90%), Postives = 362/364 (99.45%), Query Frame = 0


Query: 1   MSSEPVNINDFKELARLALPKMYYDFYSGGAEDEHTLRENIQAFYRITIRPRVLVDVSKI 60
           MSSEPVNINDFKELARLALPKMYYDFYSGGAEDEHTLRENIQAFYRITIRPRVL+DVSKI
Sbjct: 1   MSSEPVNINDFKELARLALPKMYYDFYSGGAEDEHTLRENIQAFYRITIRPRVLIDVSKI 60

Query: 61  DMSTIILGHHVSAPILVAPTAAHKLAFHEGEIATARAAAAVKTIMVLSYSSTCSIEEVAS 120
           DMST ILGHHVSAPILVAPTAAHKLAFHEGEIATARAAAAVKTIMVLSYSSTCSIEE+AS
Sbjct: 61  DMSTTILGHHVSAPILVAPTAAHKLAFHEGEIATARAAAAVKTIMVLSYSSTCSIEEIAS 120

Query: 121 SCNSVRFFQLYIFKRRDISALLVQRAERFGYKAIILTVDTPRLGRREADIKNKMIAPPVK 180
           SCNSVRFFQLYIFKRRDISALLVQRAERFGYKAIILTVDTPRLGRREADIKNKMIAPPVK
Sbjct: 121 SCNSVRFFQLYIFKRRDISALLVQRAERFGYKAIILTVDTPRLGRREADIKNKMIAPPVK 180

Query: 181 SLEGLISIDVKSDQGSKLETYANEMLDASLRWEDIGWLRSITTLPILIKGVLTHEDATKA 240
           SLEGLISIDVKSDQGSKLETYANEMLDASLRWEDIGWLRSITTLPILIKGVLTHEDATKA
Sbjct: 181 SLEGLISIDVKSDQGSKLETYANEMLDASLRWEDIGWLRSITTLPILIKGVLTHEDATKA 240

Query: 241 VEAGVDGIIVSNHGARQLDFAPATISVLEEVVHAVKGKIPVLLDGGVRRGTDVFKALALG 300
           VEAGVDGIIVSNHGARQLDFAPATISVLEEVVHAVKGKIPVLLDGGVRRGTDVFKALALG
Sbjct: 241 VEAGVDGIIVSNHGARQLDFAPATISVLEEVVHAVKGKIPVLLDGGVRRGTDVFKALALG 300

Query: 301 AQAVLIGRPVLYGLAAKGEEGVITVLEMLKNELETSMALSGCPSIKDITRSHVRTHCDNL 360
           AQAVLIGRPVLYGLAAKGEEGVITVLEMLKNELETSMALSGCPSIKDITRSHVRTH DNL
Sbjct: 301 AQAVLIGRPVLYGLAAKGEEGVITVLEMLKNELETSMALSGCPSIKDITRSHVRTHYDNL 360

Query: 361 PSML 365
           PSML
Sbjct: 361 PSML 364

[Close]

BLAST of CSPI03G28480 vs. NCBI nr
Match: XP_008460328.1 (PREDICTED: peroxisomal (S)-2-hydroxy-acid oxidase GLO4-like [Cucumis melo])

HSP 1 Score: 686.0 bits (1769), Expect = 1.7e-193
Identity = 352/364 (96.70%), Postives = 359/364 (98.63%), Query Frame = 0


Query: 1   MSSEPVNINDFKELARLALPKMYYDFYSGGAEDEHTLRENIQAFYRITIRPRVLVDVSKI 60
           MSSEPVN+NDFKELARLALPKMYYDFYSGGAEDEHTLRENIQAFYRITIRPRVLVDVSK+
Sbjct: 1   MSSEPVNVNDFKELARLALPKMYYDFYSGGAEDEHTLRENIQAFYRITIRPRVLVDVSKV 60

Query: 61  DMSTIILGHHVSAPILVAPTAAHKLAFHEGEIATARAAAAVKTIMVLSYSSTCSIEEVAS 120
           DMST ILGHH+SAPILVAPTAAHKLAFHEGE+ATARAAAAVKTIMVLSYSSTCSIEEVAS
Sbjct: 61  DMSTTILGHHISAPILVAPTAAHKLAFHEGELATARAAAAVKTIMVLSYSSTCSIEEVAS 120

Query: 121 SCNSVRFFQLYIFKRRDISALLVQRAERFGYKAIILTVDTPRLGRREADIKNKMIAPPVK 180
           SCNSVRFFQLYIFKRRDISALLVQRAER GYKAIILTVDTPRLGRREADIKNKMIAPPVK
Sbjct: 121 SCNSVRFFQLYIFKRRDISALLVQRAERCGYKAIILTVDTPRLGRREADIKNKMIAPPVK 180

Query: 181 SLEGLISIDVKSDQGSKLETYANEMLDASLRWEDIGWLRSITTLPILIKGVLTHEDATKA 240
           SLEGLISIDVKSDQGSKLETYANEMLDASL WEDIGWLRSITTLPILIKGVLTHEDATKA
Sbjct: 181 SLEGLISIDVKSDQGSKLETYANEMLDASLSWEDIGWLRSITTLPILIKGVLTHEDATKA 240

Query: 241 VEAGVDGIIVSNHGARQLDFAPATISVLEEVVHAVKGKIPVLLDGGVRRGTDVFKALALG 300
           VEAGVDGIIVSNHGARQLDFAPAT+SVLEEVVHAVKGKIPVLLDGGVRRGTDVFKALALG
Sbjct: 241 VEAGVDGIIVSNHGARQLDFAPATVSVLEEVVHAVKGKIPVLLDGGVRRGTDVFKALALG 300

Query: 301 AQAVLIGRPVLYGLAAKGEEGVITVLEMLKNELETSMALSGCPSIKDITRSHVRTHCDNL 360
           AQAVLIGRPV+YGLAAKGEEGV TV+EMLKNELETSMALSGCPSIKDITRSHVRTH DNL
Sbjct: 301 AQAVLIGRPVIYGLAAKGEEGVRTVMEMLKNELETSMALSGCPSIKDITRSHVRTHYDNL 360

Query: 361 PSML 365
           PSML
Sbjct: 361 PSML 364

[Close]

BLAST of CSPI03G28480 vs. NCBI nr
Match: TYK19117.1 (peroxisomal (S)-2-hydroxy-acid oxidase GLO4-like [Cucumis melo var. makuwa])

HSP 1 Score: 671.8 bits (1732), Expect = 3.4e-189
Identity = 352/390 (90.26%), Postives = 359/390 (92.05%), Query Frame = 0


Query: 1   MSSEPVNINDFKELARLALPKMYYDFYSGGAEDEHTLRENIQAFYRITIRPRVLVDVSKI 60
           MSSEPVN+NDFKELARLALPKMYYDFYSGGAEDEHTLRENIQAFYRITIRPRVLVDVSK+
Sbjct: 1   MSSEPVNVNDFKELARLALPKMYYDFYSGGAEDEHTLRENIQAFYRITIRPRVLVDVSKV 60

Query: 61  DMSTIILGHHVSAPILVAPTAAHKLAFHEGEIATARAAAAVKTIMVLSYSSTCSIEEVAS 120
           DMST ILGHH+SAPILVAPTAAHKLAFHEGE+ATARAAAAVKTIMVLSYSSTCSIEEVAS
Sbjct: 61  DMSTTILGHHISAPILVAPTAAHKLAFHEGELATARAAAAVKTIMVLSYSSTCSIEEVAS 120

Query: 121 SCNSVRFFQLYIFKRRDISALLVQRAERFGYKAIILTVDTPRLGRREADIKNKMIAPPVK 180
           SCNSVRFFQLYIFKRRDISALLVQRAER GYKAIILTVDTPRLGRREADIKNKMIAPPVK
Sbjct: 121 SCNSVRFFQLYIFKRRDISALLVQRAERCGYKAIILTVDTPRLGRREADIKNKMIAPPVK 180

Query: 181 SLEGLISIDVKSDQGSKLETYANEMLDASLRWE--------------------------D 240
           SLEGLISIDVKSDQGSKLETYANEMLDASL WE                          D
Sbjct: 181 SLEGLISIDVKSDQGSKLETYANEMLDASLSWEVCLMNCSHSMYVYFISGLVITFYHLQD 240

Query: 241 IGWLRSITTLPILIKGVLTHEDATKAVEAGVDGIIVSNHGARQLDFAPATISVLEEVVHA 300
           IGWLRSITTLPILIKGVLTHEDATKAVEAGVDGIIVSNHGARQLDFAPAT+SVLEEVVHA
Sbjct: 241 IGWLRSITTLPILIKGVLTHEDATKAVEAGVDGIIVSNHGARQLDFAPATVSVLEEVVHA 300

Query: 301 VKGKIPVLLDGGVRRGTDVFKALALGAQAVLIGRPVLYGLAAKGEEGVITVLEMLKNELE 360
           VKGKIPVLLDGGVRRGTDVFKALALGAQAVLIGRPV+YGLAAKGEEGV TV+EMLKNELE
Sbjct: 301 VKGKIPVLLDGGVRRGTDVFKALALGAQAVLIGRPVIYGLAAKGEEGVRTVMEMLKNELE 360

Query: 361 TSMALSGCPSIKDITRSHVRTHCDNLPSML 365
           TSMALSGCPSIKDITRSHVRTH DNLPSML
Sbjct: 361 TSMALSGCPSIKDITRSHVRTHYDNLPSML 390

[Close]

BLAST of CSPI03G28480 vs. NCBI nr
Match: KAA0053522.1 (peroxisomal (S)-2-hydroxy-acid oxidase GLO4-like [Cucumis melo var. makuwa])

HSP 1 Score: 671.4 bits (1731), Expect = 4.4e-189
Identity = 351/390 (90.00%), Postives = 359/390 (92.05%), Query Frame = 0


Query: 1   MSSEPVNINDFKELARLALPKMYYDFYSGGAEDEHTLRENIQAFYRITIRPRVLVDVSKI 60
           MSSEPVN+NDFKELARLALPKMYYDFYSGGAEDEHTLRENIQAFYRITIRPRVLVDVSK+
Sbjct: 1   MSSEPVNVNDFKELARLALPKMYYDFYSGGAEDEHTLRENIQAFYRITIRPRVLVDVSKV 60

Query: 61  DMSTIILGHHVSAPILVAPTAAHKLAFHEGEIATARAAAAVKTIMVLSYSSTCSIEEVAS 120
           DMST ILGHH+SAPILVAPTAAHKLAFHEGE+ATARAAAAVKTIMVLSYSSTCSIEEVAS
Sbjct: 61  DMSTTILGHHISAPILVAPTAAHKLAFHEGELATARAAAAVKTIMVLSYSSTCSIEEVAS 120

Query: 121 SCNSVRFFQLYIFKRRDISALLVQRAERFGYKAIILTVDTPRLGRREADIKNKMIAPPVK 180
           SCNSVRFFQLYIFKRRDISALLVQRAER GYKAIILTVDTPRLGRREADIKNKMIAPPVK
Sbjct: 121 SCNSVRFFQLYIFKRRDISALLVQRAERCGYKAIILTVDTPRLGRREADIKNKMIAPPVK 180

Query: 181 SLEGLISIDVKSDQGSKLETYANEMLDASLRWE--------------------------D 240
           SLEGLISIDVKSDQGSKLETYANEMLDASL WE                          D
Sbjct: 181 SLEGLISIDVKSDQGSKLETYANEMLDASLSWEVCLMNCSHSMYVYFISGLVITFYHLQD 240

Query: 241 IGWLRSITTLPILIKGVLTHEDATKAVEAGVDGIIVSNHGARQLDFAPATISVLEEVVHA 300
           IGWLRSITTLPILIKG+LTHEDATKAVEAGVDGIIVSNHGARQLDFAPAT+SVLEEVVHA
Sbjct: 241 IGWLRSITTLPILIKGILTHEDATKAVEAGVDGIIVSNHGARQLDFAPATVSVLEEVVHA 300

Query: 301 VKGKIPVLLDGGVRRGTDVFKALALGAQAVLIGRPVLYGLAAKGEEGVITVLEMLKNELE 360
           VKGKIPVLLDGGVRRGTDVFKALALGAQAVLIGRPV+YGLAAKGEEGV TV+EMLKNELE
Sbjct: 301 VKGKIPVLLDGGVRRGTDVFKALALGAQAVLIGRPVIYGLAAKGEEGVRTVMEMLKNELE 360

Query: 361 TSMALSGCPSIKDITRSHVRTHCDNLPSML 365
           TSMALSGCPSIKDITRSHVRTH DNLPSML
Sbjct: 361 TSMALSGCPSIKDITRSHVRTHYDNLPSML 390

[Close]

BLAST of CSPI03G28480 vs. NCBI nr
Match: XP_038880952.1 (peroxisomal (S)-2-hydroxy-acid oxidase GLO4-like isoform X2 [Benincasa hispida] >XP_038880960.1 peroxisomal (S)-2-hydroxy-acid oxidase GLO4-like isoform X2 [Benincasa hispida])

HSP 1 Score: 657.1 bits (1694), Expect = 8.6e-185
Identity = 336/364 (92.31%), Postives = 351/364 (96.43%), Query Frame = 0


Query: 1   MSSEPVNINDFKELARLALPKMYYDFYSGGAEDEHTLRENIQAFYRITIRPRVLVDVSKI 60
           MSSEPVN+NDFKELARLALPKMYYDFY+GGAEDEHTLRENIQAFYRITIRPRVLVDVSKI
Sbjct: 1   MSSEPVNVNDFKELARLALPKMYYDFYAGGAEDEHTLRENIQAFYRITIRPRVLVDVSKI 60

Query: 61  DMSTIILGHHVSAPILVAPTAAHKLAFHEGEIATARAAAAVKTIMVLSYSSTCSIEEVAS 120
           DMST ILGH +S P+LVAPTAAHKLAFHEGE+ATARAAAA  TIMVLSYSSTCSIEEVAS
Sbjct: 61  DMSTTILGHRISTPMLVAPTAAHKLAFHEGELATARAAAAANTIMVLSYSSTCSIEEVAS 120

Query: 121 SCNSVRFFQLYIFKRRDISALLVQRAERFGYKAIILTVDTPRLGRREADIKNKMIAPPVK 180
           SCN+VRFFQLYIF+RRDISALLVQRAE+FGYKAIILTVDTPRLGRREADIKNKMIAPPVK
Sbjct: 121 SCNAVRFFQLYIFRRRDISALLVQRAEKFGYKAIILTVDTPRLGRREADIKNKMIAPPVK 180

Query: 181 SLEGLISIDVKSDQGSKLETYANEMLDASLRWEDIGWLRSITTLPILIKGVLTHEDATKA 240
           +LEGLISI+V +DQGSKLETYANEMLDASL WEDIGWLRSITTLPILIKGVLTHEDATKA
Sbjct: 181 NLEGLISINVNTDQGSKLETYANEMLDASLCWEDIGWLRSITTLPILIKGVLTHEDATKA 240

Query: 241 VEAGVDGIIVSNHGARQLDFAPATISVLEEVVHAVKGKIPVLLDGGVRRGTDVFKALALG 300
           VEAGVDGIIVSNHGARQLDFAPAT+SV+EEVVHAV+GKIPVLLDGGVRRGTDVFKALALG
Sbjct: 241 VEAGVDGIIVSNHGARQLDFAPATVSVIEEVVHAVRGKIPVLLDGGVRRGTDVFKALALG 300

Query: 301 AQAVLIGRPVLYGLAAKGEEGVITVLEMLKNELETSMALSGCPSIKDITRSHVRTHCDNL 360
           AQAVLIGRPVL+GLAAKGEEGV  VLEMLKNELETSMALSGCP IKDITRSHVRTH D L
Sbjct: 301 AQAVLIGRPVLFGLAAKGEEGVRKVLEMLKNELETSMALSGCPCIKDITRSHVRTHYDKL 360

Query: 361 PSML 365
            SML
Sbjct: 361 QSML 364

[Close]

BLAST of CSPI03G28480 vs. TAIR 10
Match: AT3G14130.1 (Aldolase-type TIM barrel family protein )

HSP 1 Score: 530.0 bits (1364), Expect = 1.5e-150
Identity = 258/359 (71.87%), Postives = 318/359 (88.58%), Query Frame = 0


Query: 6   VNINDFKELARLALPKMYYDFYSGGAEDEHTLRENIQAFYRITIRPRVLVDVSKIDMSTI 65
           VN+++F+ELA+ ALPKMYYDFY+GGAED+HTL EN+QAF RI  RPRVLVDVS IDMST 
Sbjct: 5   VNVDEFQELAKQALPKMYYDFYNGGAEDQHTLNENVQAFRRIMFRPRVLVDVSNIDMSTS 64

Query: 66  ILGHHVSAPILVAPTAAHKLAFHEGEIATARAAAAVKTIMVLSYSSTCSIEEVASSCNSV 125
           +LG+ +SAPI++APTA HKLA  +GEIATA+AAAA  TIM++S+ STC+IEEVASSCN+V
Sbjct: 65  MLGYPISAPIMIAPTAMHKLAHPKGEIATAKAAAACNTIMIVSFMSTCTIEEVASSCNAV 124

Query: 126 RFFQLYIFKRRDISALLVQRAERFGYKAIILTVDTPRLGRREADIKNKMIAPPVKSLEGL 185
           RF Q+Y++KRRD++A +V+RAE+ G+KAI+LTVD PRLGRREADIKNKMI+P +K+ EGL
Sbjct: 125 RFLQIYVYKRRDVTAQIVKRAEKAGFKAIVLTVDVPRLGRREADIKNKMISPQLKNFEGL 184

Query: 186 ISIDVKSDQGSKLETYANEMLDASLRWEDIGWLRSITTLPILIKGVLTHEDATKAVEAGV 245
           +S +V+ ++GS +E +A+   DASL W+DI WLRSIT LPIL+KG+LT EDA KAVEAGV
Sbjct: 185 VSTEVRPNEGSGVEAFASSAFDASLSWKDIEWLRSITKLPILVKGLLTREDALKAVEAGV 244

Query: 246 DGIIVSNHGARQLDFAPATISVLEEVVHAVKGKIPVLLDGGVRRGTDVFKALALGAQAVL 305
           DGI+VSNHGARQLD++PATI+VLEEVVHAVKG+IPVLLDGGVRRGTDVFKALALGAQAVL
Sbjct: 245 DGIVVSNHGARQLDYSPATITVLEEVVHAVKGRIPVLLDGGVRRGTDVFKALALGAQAVL 304

Query: 306 IGRPVLYGLAAKGEEGVITVLEMLKNELETSMALSGCPSIKDITRSHVRTHCDNLPSML 365
           IGRP++YGLAAKGE+GV  V++MLKNE E +MALSGCP+I D+TR+HVRT  + + SML
Sbjct: 305 IGRPIVYGLAAKGEDGVKKVIDMLKNEFEITMALSGCPTIDDVTRNHVRTENERIKSML 363

[Close]

BLAST of CSPI03G28480 vs. TAIR 10
Match: AT3G14150.1 (Aldolase-type TIM barrel family protein )

HSP 1 Score: 518.8 bits (1335), Expect = 3.4e-147
Identity = 255/359 (71.03%), Postives = 310/359 (86.35%), Query Frame = 0


Query: 6   VNINDFKELARLALPKMYYDFYSGGAEDEHTLRENIQAFYRITIRPRVLVDVSKIDMSTI 65
           VN+++F+ELA+ ALPKMYYDFY+GGAED+HTL EN+QAF RI  RPRVLVDVSKIDMST 
Sbjct: 5   VNVDEFQELAKQALPKMYYDFYNGGAEDQHTLNENVQAFRRIMFRPRVLVDVSKIDMSTK 64

Query: 66  ILGHHVSAPILVAPTAAHKLAFHEGEIATARAAAAVKTIMVLSYSSTCSIEEVASSCNSV 125
           ILG+ +SAPI++APT  HKLA  EGE ATA+AAAA  TIM++SY S+C+ EE+ASSCN+V
Sbjct: 65  ILGYPISAPIMIAPTGNHKLAHPEGETATAKAAAACNTIMIVSYMSSCTFEEIASSCNAV 124

Query: 126 RFFQLYIFKRRDISALLVQRAERFGYKAIILTVDTPRLGRREADIKNKMIAPPVKSLEGL 185
           RF Q+Y++KRRDI+A +V+RAE+ G+KAI+LTVD PRLGRREADIKNKMI+P +K+ EGL
Sbjct: 125 RFLQIYVYKRRDITAQVVKRAEKAGFKAIVLTVDVPRLGRREADIKNKMISPQLKNFEGL 184

Query: 186 ISIDVKSDQGSKLETYANEMLDASLRWEDIGWLRSITTLPILIKGVLTHEDATKAVEAGV 245
            S +V+  +GS ++ +A+   DAS  W+DI WLRSIT LPIL+KG+LT EDA KAVEAGV
Sbjct: 185 FSTEVRPSKGSGVQAFASRAFDASFSWKDIEWLRSITELPILVKGILTREDALKAVEAGV 244

Query: 246 DGIIVSNHGARQLDFAPATISVLEEVVHAVKGKIPVLLDGGVRRGTDVFKALALGAQAVL 305
           DGIIVSNHG RQLD++PATI+VLEEVV  V+G+IPVLLDGGVRRGTDVFKALALGAQAVL
Sbjct: 245 DGIIVSNHGGRQLDYSPATITVLEEVVQVVRGRIPVLLDGGVRRGTDVFKALALGAQAVL 304

Query: 306 IGRPVLYGLAAKGEEGVITVLEMLKNELETSMALSGCPSIKDITRSHVRTHCDNLPSML 365
           IGRP++YGLAAKGE+GV  V++MLKNE E +MALSGCP+I DITR+HVRT  + L SML
Sbjct: 305 IGRPIIYGLAAKGEDGVKKVIDMLKNEFEITMALSGCPTIDDITRNHVRTENERLHSML 363

[Close]

BLAST of CSPI03G28480 vs. TAIR 10
Match: AT3G14150.2 (Aldolase-type TIM barrel family protein )

HSP 1 Score: 518.8 bits (1335), Expect = 3.4e-147
Identity = 255/359 (71.03%), Postives = 310/359 (86.35%), Query Frame = 0


Query: 6   VNINDFKELARLALPKMYYDFYSGGAEDEHTLRENIQAFYRITIRPRVLVDVSKIDMSTI 65
           VN+++F+ELA+ ALPKMYYDFY+GGAED+HTL EN+QAF RI  RPRVLVDVSKIDMST 
Sbjct: 5   VNVDEFQELAKQALPKMYYDFYNGGAEDQHTLNENVQAFRRIMFRPRVLVDVSKIDMSTK 64

Query: 66  ILGHHVSAPILVAPTAAHKLAFHEGEIATARAAAAVKTIMVLSYSSTCSIEEVASSCNSV 125
           ILG+ +SAPI++APT  HKLA  EGE ATA+AAAA  TIM++SY S+C+ EE+ASSCN+V
Sbjct: 65  ILGYPISAPIMIAPTGNHKLAHPEGETATAKAAAACNTIMIVSYMSSCTFEEIASSCNAV 124

Query: 126 RFFQLYIFKRRDISALLVQRAERFGYKAIILTVDTPRLGRREADIKNKMIAPPVKSLEGL 185
           RF Q+Y++KRRDI+A +V+RAE+ G+KAI+LTVD PRLGRREADIKNKMI+P +K+ EGL
Sbjct: 125 RFLQIYVYKRRDITAQVVKRAEKAGFKAIVLTVDVPRLGRREADIKNKMISPQLKNFEGL 184

Query: 186 ISIDVKSDQGSKLETYANEMLDASLRWEDIGWLRSITTLPILIKGVLTHEDATKAVEAGV 245
            S +V+  +GS ++ +A+   DAS  W+DI WLRSIT LPIL+KG+LT EDA KAVEAGV
Sbjct: 185 FSTEVRPSKGSGVQAFASRAFDASFSWKDIEWLRSITELPILVKGILTREDALKAVEAGV 244

Query: 246 DGIIVSNHGARQLDFAPATISVLEEVVHAVKGKIPVLLDGGVRRGTDVFKALALGAQAVL 305
           DGIIVSNHG RQLD++PATI+VLEEVV  V+G+IPVLLDGGVRRGTDVFKALALGAQAVL
Sbjct: 245 DGIIVSNHGGRQLDYSPATITVLEEVVQVVRGRIPVLLDGGVRRGTDVFKALALGAQAVL 304

Query: 306 IGRPVLYGLAAKGEEGVITVLEMLKNELETSMALSGCPSIKDITRSHVRTHCDNLPSML 365
           IGRP++YGLAAKGE+GV  V++MLKNE E +MALSGCP+I DITR+HVRT  + L SML
Sbjct: 305 IGRPIIYGLAAKGEDGVKKVIDMLKNEFEITMALSGCPTIDDITRNHVRTENERLHSML 363

[Close]

BLAST of CSPI03G28480 vs. TAIR 10
Match: AT3G14420.1 (Aldolase-type TIM barrel family protein )

HSP 1 Score: 410.6 bits (1054), Expect = 1.3e-114
Identity = 211/358 (58.94%), Postives = 270/358 (75.42%), Query Frame = 0


Query: 4   EPVNINDFKELARLALPKMYYDFYSGGAEDEHTLRENIQAFYRITIRPRVLVDVSKIDMS 63
           E  N+ ++  +A+  LPKM YD+Y+ GAED+ TL+EN  AF RI  RPR+L+DVSKIDM+
Sbjct: 2   EITNVTEYDAIAKQKLPKMVYDYYASGAEDQWTLQENRNAFARILFRPRILIDVSKIDMT 61

Query: 64  TIILGHHVSAPILVAPTAAHKLAFHEGEIATARAAAAVKTIMVLSYSSTCSIEEVASSCN 123
           T +LG  +S PI+VAPTA  K+A  +GE ATARAA+A  TIM LS  +T S+EEVAS+  
Sbjct: 62  TTVLGFKISMPIMVAPTAMQKMAHPDGEYATARAASAAGTIMTLSSWATSSVEEVASTGP 121

Query: 124 SVRFFQLYIFKRRDISALLVQRAERFGYKAIILTVDTPRLGRREADIKNKMIAPP---VK 183
            +RFFQLY++K R++   LV+RAER G+KAI LTVDTPRLGRRE+DIKN+   PP   +K
Sbjct: 122 GIRFFQLYVYKNRNVVEQLVRRAERAGFKAIALTVDTPRLGRRESDIKNRFTLPPNLTLK 181

Query: 184 SLEGLISIDVKSDQGSKLETYANEMLDASLRWEDIGWLRSITTLPILIKGVLTHEDATKA 243
           + EGL    +     S L +Y    +D +L W+D+ WL++IT LPIL+KGVLT EDA  A
Sbjct: 182 NFEGLDLGKMDEANDSGLASYVAGQIDRTLSWKDVQWLQTITKLPILVKGVLTGEDARIA 241

Query: 244 VEAGVDGIIVSNHGARQLDFAPATISVLEEVVHAVKGKIPVLLDGGVRRGTDVFKALALG 303
           ++AG  GIIVSNHGARQLD+ PATIS LEEVV A +G+IPV LDGGVRRGTDVFKALALG
Sbjct: 242 IQAGAAGIIVSNHGARQLDYVPATISALEEVVKATQGRIPVFLDGGVRRGTDVFKALALG 301

Query: 304 AQAVLIGRPVLYGLAAKGEEGVITVLEMLKNELETSMALSGCPSIKDITRSHVRTHCD 359
           A  + IGRPV++ LAA+GE GV  VL+ML++E E +MALSGC S+K+I+R+H+ T  D
Sbjct: 302 ASGIFIGRPVVFSLAAEGEAGVRKVLQMLRDEFELTMALSGCRSLKEISRNHITTEWD 359

[Close]

BLAST of CSPI03G28480 vs. TAIR 10
Match: AT3G14420.2 (Aldolase-type TIM barrel family protein )

HSP 1 Score: 410.6 bits (1054), Expect = 1.3e-114
Identity = 211/358 (58.94%), Postives = 270/358 (75.42%), Query Frame = 0


Query: 4   EPVNINDFKELARLALPKMYYDFYSGGAEDEHTLRENIQAFYRITIRPRVLVDVSKIDMS 63
           E  N+ ++  +A+  LPKM YD+Y+ GAED+ TL+EN  AF RI  RPR+L+DVSKIDM+
Sbjct: 2   EITNVTEYDAIAKQKLPKMVYDYYASGAEDQWTLQENRNAFARILFRPRILIDVSKIDMT 61

Query: 64  TIILGHHVSAPILVAPTAAHKLAFHEGEIATARAAAAVKTIMVLSYSSTCSIEEVASSCN 123
           T +LG  +S PI+VAPTA  K+A  +GE ATARAA+A  TIM LS  +T S+EEVAS+  
Sbjct: 62  TTVLGFKISMPIMVAPTAMQKMAHPDGEYATARAASAAGTIMTLSSWATSSVEEVASTGP 121

Query: 124 SVRFFQLYIFKRRDISALLVQRAERFGYKAIILTVDTPRLGRREADIKNKMIAPP---VK 183
            +RFFQLY++K R++   LV+RAER G+KAI LTVDTPRLGRRE+DIKN+   PP   +K
Sbjct: 122 GIRFFQLYVYKNRNVVEQLVRRAERAGFKAIALTVDTPRLGRRESDIKNRFTLPPNLTLK 181

Query: 184 SLEGLISIDVKSDQGSKLETYANEMLDASLRWEDIGWLRSITTLPILIKGVLTHEDATKA 243
           + EGL    +     S L +Y    +D +L W+D+ WL++IT LPIL+KGVLT EDA  A
Sbjct: 182 NFEGLDLGKMDEANDSGLASYVAGQIDRTLSWKDVQWLQTITKLPILVKGVLTGEDARIA 241

Query: 244 VEAGVDGIIVSNHGARQLDFAPATISVLEEVVHAVKGKIPVLLDGGVRRGTDVFKALALG 303
           ++AG  GIIVSNHGARQLD+ PATIS LEEVV A +G+IPV LDGGVRRGTDVFKALALG
Sbjct: 242 IQAGAAGIIVSNHGARQLDYVPATISALEEVVKATQGRIPVFLDGGVRRGTDVFKALALG 301

Query: 304 AQAVLIGRPVLYGLAAKGEEGVITVLEMLKNELETSMALSGCPSIKDITRSHVRTHCD 359
           A  + IGRPV++ LAA+GE GV  VL+ML++E E +MALSGC S+K+I+R+H+ T  D
Sbjct: 302 ASGIFIGRPVVFSLAAEGEAGVRKVLQMLRDEFELTMALSGCRSLKEISRNHITTEWD 359

The following BLAST results are available for this feature:

BLAST of CSPI03G28480 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LJH5	2.1e-149	71.87	Peroxisomal (S)-2-hydroxy-acid oxidase GLO4 OS=Arabidopsis thaliana OX=3702 GN=G...	[more]
Q24JJ8	4.8e-146	71.03	Peroxisomal (S)-2-hydroxy-acid oxidase GLO3 OS=Arabidopsis thaliana OX=3702 GN=G...	[more]
Q8H3I4	7.5e-131	64.82	Peroxisomal (S)-2-hydroxy-acid oxidase GLO4 OS=Oryza sativa subsp. japonica OX=3...	[more]
B8B8K5	1.8e-129	64.54	Peroxisomal (S)-2-hydroxy-acid oxidase GLO4 OS=Oryza sativa subsp. indica OX=399...	[more]
B8B7C5	5.7e-115	59.73	Peroxisomal (S)-2-hydroxy-acid oxidase GLO5 OS=Oryza sativa subsp. indica OX=399...	[more]

BLAST of CSPI03G28480 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LEF4	2.4e-196	98.63	(S)-2-hydroxy-acid oxidase OS=Cucumis sativus OX=3659 GN=Csa_3G645990 PE=3 SV=1	[more]
A0A1S3CCP5	8.4e-194	96.70	(S)-2-hydroxy-acid oxidase OS=Cucumis melo OX=3656 GN=LOC103499183 PE=3 SV=1	[more]
A0A5D3D6C7	1.6e-189	90.26	(S)-2-hydroxy-acid oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff...	[more]
A0A5A7UCM3	2.1e-189	90.00	(S)-2-hydroxy-acid oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaff...	[more]
A0A6J1GVS5	6.7e-183	92.03	(S)-2-hydroxy-acid oxidase OS=Cucurbita moschata OX=3662 GN=LOC111457615 PE=3 SV...	[more]

BLAST of CSPI03G28480 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004144438.2	7.5e-197	98.90	peroxisomal (S)-2-hydroxy-acid oxidase GLO4 [Cucumis sativus] >XP_031738757.1 pe...	[more]
XP_008460328.1	1.7e-193	96.70	PREDICTED: peroxisomal (S)-2-hydroxy-acid oxidase GLO4-like [Cucumis melo]	[more]
TYK19117.1	3.4e-189	90.26	peroxisomal (S)-2-hydroxy-acid oxidase GLO4-like [Cucumis melo var. makuwa]	[more]
KAA0053522.1	4.4e-189	90.00	peroxisomal (S)-2-hydroxy-acid oxidase GLO4-like [Cucumis melo var. makuwa]	[more]
XP_038880952.1	8.6e-185	92.31	peroxisomal (S)-2-hydroxy-acid oxidase GLO4-like isoform X2 [Benincasa hispida] ...	[more]

BLAST of CSPI03G28480 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G14130.1	1.5e-150	71.87	Aldolase-type TIM barrel family protein	[more]
AT3G14150.1	3.4e-147	71.03	Aldolase-type TIM barrel family protein	[more]
AT3G14150.2	3.4e-147	71.03	Aldolase-type TIM barrel family protein	[more]
AT3G14420.1	1.3e-114	58.94	Aldolase-type TIM barrel family protein	[more]
AT3G14420.2	1.3e-114	58.94	Aldolase-type TIM barrel family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000262	FMN-dependent dehydrogenase	PFAM	PF01070	FMN_dh	coord: 25..364 e-value: 2.2E-133 score: 444.6
IPR012133	Alpha-hydroxy acid dehydrogenase, FMN-dependent	PIRSF	PIRSF000138	Al-hdrx_acd_dh	coord: 7..367 e-value: 1.8E-137 score: 456.3
IPR012133	Alpha-hydroxy acid dehydrogenase, FMN-dependent	CDD	cd02809	alpha_hydroxyacid_oxid_FMN	coord: 19..360 e-value: 4.20643E-157 score: 441.501
IPR013785	Aldolase-type TIM barrel	GENE3D	3.20.20.70	Aldolase class I	coord: 13..373 e-value: 2.3E-158 score: 528.7
None	No IPR available	PANTHER	PTHR10578:SF116	PEROXISOMAL (S)-2-HYDROXY-ACID OXIDASE GLO4-LIKE	coord: 11..364
None	No IPR available	PANTHER	PTHR10578	S -2-HYDROXY-ACID OXIDASE-RELATED	coord: 11..364
None	No IPR available	SUPERFAMILY	51395	FMN-linked oxidoreductases	coord: 15..365
IPR008259	FMN-dependent alpha-hydroxy acid dehydrogenase, active site	PROSITE	PS00557	FMN_HYDROXY_ACID_DH_1	coord: 261..267
IPR037396	FMN hydroxy acid dehydrogenase domain	PROSITE	PS51349	FMN_HYDROXY_ACID_DH_2	coord: 10..368 score: 109.75502

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G28480.1	CSPI03G28480.1	mRNA
CSPI03G28480.2	CSPI03G28480.2	mRNA
CSPI03G28480.3	CSPI03G28480.3	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009854	oxidative photosynthetic carbon pathway
cellular_component	GO:0005777	peroxisome
molecular_function	GO:0010181	FMN binding
molecular_function	GO:0003973	(S)-2-hydroxy-acid oxidase activity
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0016491	oxidoreductase activity