CSPI03G28140 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI03G28140
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	mediator of RNA polymerase II transcription subunit 8 isoform X1
Location	Chr3: 25743080 .. 25747263 (-)
RNA-Seq Expression	CSPI03G28140
Synteny	CSPI03G28140

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

AATTTATTTTATCAACGCCACTTTATAAGAAAGAACATGAGAATCAAATAGTTATTGAAAATTACATTTAATTTGGGATCAAATAGATATACGCTTCCAGGTAAAACGGTATCAATCGGAGCACCTCAGGCAAGCCTGGCCTCGCAGCCAGACCCGAGCGAGATCTACACTCCCCGTACCCGTTGTACGTTACTCGCATCGAACAGGCCAACTGGTGTCTTTCGCTAATGGCGACCATTGACGGGGCGGTGCAGAATCAGCCTCAGCAGCAACAGCCTCTTCCGGCGCAGCAGGTGGTTTTCGCGGAAAGGCTAAACCAGGCAGTTCAGCAACAGTTGAACCTGGAGGCTCTGAAGAATCGAGCTACTACTCTCTTTAAAGCCATTTCTCGGATCCTTGAAGATTTTGATGTCTATGGTCGCACCAATACCACACCTAAATGGTCTACTTTCATTTTTCGCCTAGTTTTCTCTTGTGATATTTTCCAAATATGCTTGAATTTTTATGTTCGTCATTGGCGATTGATAGCACGTTAGTGTTAGAAAGTAATTTTTTTCTCTATCTCGTGGATTTACATACTGATTATTTTCTAGACATCAAAGCTTCTTAATATCTTTCTATCTATCTTTCTATTTATATTTCGATATTCTCCTCTACTGGCCGACTGTATAGTTGATTTTTACGCCAGGCAAGACATACTGGGGCAGTATTCTATGGTGAATCTCGAGCTTTTCAACATTGTTGACGAAATCAAGAAAGTTTCGAAGGCTTTCGTTGTACATCCAAAGAATGTTAATGCAGAGAACTCTACAAGTATGTCCCTGCATCCCTTCCAAGTATTTGCTGAAGTAAAAGGAAGAAATGCTTAGTTTTTGTTTTGTACGCTTGAACAATCATTCATTTATTTGTAAATTTTGATTTCAACTAATTTTTCTTTCTTTTTTCTTTTTGTGAGCCCAGTACTACCAGTTATGTTGTCCTCAAAACTATTGCCTGAGATGGAGGTAGATGACAATTCGAAACGAGAGCAGTTGCTTCTTGGGATGCACAACATGCCAGTATCTACACAGATTGAGAAATTAAAGGTACTAGAAGTTTGTTAAATAATTATAGGTCGTAGAACTCCCCGTGTTCTAAAGAAATTCATTAAATCACATACTTTCTTATGTATCTGGCATTTAGGTTTACTTTTCTTACAGACTAGAATTGAAATGATTGGAGCAGCCTGTGAAAGTGCTGAAAAAATATTAGCTGAAACTCGAAAAGCATATTGCTTCGGAATGCGTCAAGGGCCAGCAATAGCTCCAACCTTAGATAAGGCTCAAGCTGCAAAAATTCAGGAGCAAGAAAATTTACTCCGAGCTGCTGTTAACTTTGGGGAAGGTGATATTTTTTTACTTTTATAGTTATTGGTTAGTATCTTAGTTTTAATTATTTGTAGTTCATATATTGTTTAGTACTGCATCATGGCTTGTAAGTCCACTCATGATCGACATTTACCAAAAAAAGCAATATTATATTTAGACTTCTTGCCAACTTGTCACATATCTATGAAGATTACTGATTTGTATATTTATTTCTTTATTCATGATATCTTTATCAATTTTTTTGTGCATAATTGAATATGAACATTCTAACTTGTGTAACAGCCATTTCTCCATTGTTGTATGAATTAGACACAATAACTTGCTTTTTGGGAGAGTTGATTTGAATGACTAAATTGTTTGAAATTTTGTGAAAGAGTTGCTGAGGGAGAAAGGAGAAGTGGAGTGTTGTAGAATGGGAGTGATGCGACCTCGTAAAATGACCATATGAATTATGGAGGATGCATATATGGCAAAAAATGCTCTGTTCCTTCGGTCAATGAGGCTGTAGGGATCCTAGCTTAGTTGGATCCCCTAAACCAATTGATCTATCATCCTTCTTCATCCTTCCCAAACACTTGAAGGCCCCAACTAAGTAGCAAGCCCACACTATGAGGCTTGGAAAACTGATTTAATTCTTGCAAAAGATGTGGGTATAAGGTGTTAAAAGTAATATCTGAAAAAGAGGAACCATTATGGATTGACCTAATGGTAAATAGGGGACATGACTTTGATAAAGGGTTGAGAGGTCACAGGCTCAGTCCCTGATCTACCTAGCTAGAATTTAGTGTCCTATGAGTTTTCTCGACATCCAAATGTTGTAGGGTTAAATCAATTGTTTTGTGAGATTAGTCGAGATGTGCATAAGCTGATCTAAACCCTTACAAATTTCAAAAAAAAAAAAAAGAAAAGAAAAGAAAAAGAGGGTTGTCAACTAGTAATCTTTTCTATTTGAAGAGGCTTGTTTCCATTTAAAAAAAAAACAAGTAGCCTTTTCTATTAAATTCCAAGATCTAATATTCTCCTTCAAATATCTCTCTTGCAAAAATTCTCAACACACAATAGAATGCAAGATCTTCAATCATTTCCTCCGTACTGTTTCATTTTTCTCTCTTTTGTGCTCTTTAGAGCAAATGGCCAAAAGGGAGATGTTCTTTGTTGTCAAGCTTTGAGCCTAATGTAATAGTTGCAAAGTGGTTTGAGCTACAAACATTTCAGTTGTGTGATTGTATCTTGGAGGAGACATGTTAGACAATATCTGGTGCCTTGGTATAAAGGAATACGTAGTCTCTTAAAAGACAGTGAGATACATGCACCTCAATGTAATTGTTTACGCGTTTGTTTCTCAATATTTACTCCAATTTCTAGGTTATTTGTAAACCATTAGTTCGTTAGTTAAGCTGTTGGGCTTCGGCAAGACAAAAGGCTCATCAGATCCTTCGTTAGATTCCATATTTCTCTCAGTTTCAGTTACAAAAGTTTTTTGTATTGACAATAGAGCATTATTTTACGCAACTGATGGCCCTTTCTTTAGTGGCCTACCTTTATGTAGGATTATTCTTTTTGGATCCTGGTAAATTCTTTCATTTTTTTTTCTCAAATGTTGTTTTCTCATTATAAAGAAAAATAAAATAAAATAAAATAAAAGAGAAGTCCTTGATGCCGATGCATATGATTTATAACATTATTTTTTGGTTTACTTGGATCTATTGTTAAAACTTGGCTCAAACTTGAAGTTTCATTTGCTTCTATATTTGTCCTCTCCTAATTTTTTCAATAAGTGCAGTAGTCATGCCTTGTAAATGAATCTTATATTTACATGCAGGATTAAGATTACCTGGTGAGCAACGACAAATTACGCCAACACTTCCGATGCATTTGATAGATGTGCTGAATGCAGGTGATGGAGTGCAGAGTTTTAACGAAACGTCCGGTAACGTTCATTTGTCTTTTCAAACATAAATGGTTTATAGATTAATTTACTAAATTGCACTAACTTGTAATTGTTTAACAAAATCTCTTTCAGGTATGTATGCAAAGAGTACTCCTCTAACGTCAAATAATATTAGTAGTCAAGGAGCAGTGATACAGGTTAGATGATGTCCTTGCTCCTTGAAATGGTTTCTTAATGATTTATTTGCATCTTCTTAAGTTATAGAATGTAAACTAAGTTCTCCAAGGGGAAGCAACTTTTGGCTAACAGATTTCGTTTCATTCGAGGTTCTCTTATGTACGTGATTGAATTATCTTGTATCAGTTGGTTCCTAATTTTATTAGGATTTCTTTGGTTATGACTTATGAGTTGTATCTACCATTCATTCTTCCTTGAAAGATGACGTTAGATAGTATAGTACTGTTAGGAATAAAATAATTGAACATTTTCTTGCCGACTGTCTCCTATTCTCGCAATTCTTTCTTTTCCCTGCTCTTCCTATGATTATTTGCATTATTATTGGTGTTGAACTCGGGCACTTGGATTGTTTGGTTTGCACATTTAGATATAATAAGAACTCTACATGTTATTTTTGAGGCTAATGTATGGATCAAAATTAATAGGGTAATGATAGAACACCAAGTAGTGTGTTCTATTATTTTATTAAACAACCAAACTTTTACAGAAAATAGTACTAACACAACCTTGAAAACAAAGAACAACACAAGAACTAATATAGAAATGAACCAAGTTCAACATAAAACATCAAAACAGAAAAGTACTTCACGATAATGGAAATAACTTGCAACAGACTACAAAAACTGAAAACTTTTTGCATTCCCTGCTCTCCAGGAAATTACAGCCCTCCACTTCCAGAAACTGATTAA

mRNA sequence

AATTTATTTTATCAACGCCACTTTATAAGAAAGAACATGAGAATCAAATAGTTATTGAAAATTACATTTAATTTGGGATCAAATAGATATACGCTTCCAGGTAAAACGGTATCAATCGGAGCACCTCAGGCAAGCCTGGCCTCGCAGCCAGACCCGAGCGAGATCTACACTCCCCGTACCCGTTGTACGTTACTCGCATCGAACAGGCCAACTGGTGTCTTTCGCTAATGGCGACCATTGACGGGGCGGTGCAGAATCAGCCTCAGCAGCAACAGCCTCTTCCGGCGCAGCAGGTGGTTTTCGCGGAAAGGCTAAACCAGGCAGTTCAGCAACAGTTGAACCTGGAGGCTCTGAAGAATCGAGCTACTACTCTCTTTAAAGCCATTTCTCGGATCCTTGAAGATTTTGATGTCTATGGTCGCACCAATACCACACCTAAATGGCAAGACATACTGGGGCAGTATTCTATGGTGAATCTCGAGCTTTTCAACATTGTTGACGAAATCAAGAAAGTTTCGAAGGCTTTCGTTGTACATCCAAAGAATGTTAATGCAGAGAACTCTACAATACTACCAGTTATGTTGTCCTCAAAACTATTGCCTGAGATGGAGGTAGATGACAATTCGAAACGAGAGCAGTTGCTTCTTGGGATGCACAACATGCCAGTATCTACACAGATTGAGAAATTAAAGACTAGAATTGAAATGATTGGAGCAGCCTGTGAAAGTGCTGAAAAAATATTAGCTGAAACTCGAAAAGCATATTGCTTCGGAATGCGTCAAGGGCCAGCAATAGCTCCAACCTTAGATAAGGCTCAAGCTGCAAAAATTCAGGAGCAAGAAAATTTACTCCGAGCTGCTGTTAACTTTGGGGAAGGATTAAGATTACCTGGTGAGCAACGACAAATTACGCCAACACTTCCGATGCATTTGATAGATGTGCTGAATGCAGGTGATGGAGTGCAGAGTTTTAACGAAACGTCCGGTATGTATGCAAAGAGTACTCCTCTAACGTCAAATAATATTAGTAGTCAAGGAGCAGTGATACAGGGGAAGCAACTTTTGGCTAACAGATTTCGTTTCATTCGAGGAAATTACAGCCCTCCACTTCCAGAAACTGATTAA

Coding sequence (CDS)

ATGGCGACCATTGACGGGGCGGTGCAGAATCAGCCTCAGCAGCAACAGCCTCTTCCGGCGCAGCAGGTGGTTTTCGCGGAAAGGCTAAACCAGGCAGTTCAGCAACAGTTGAACCTGGAGGCTCTGAAGAATCGAGCTACTACTCTCTTTAAAGCCATTTCTCGGATCCTTGAAGATTTTGATGTCTATGGTCGCACCAATACCACACCTAAATGGCAAGACATACTGGGGCAGTATTCTATGGTGAATCTCGAGCTTTTCAACATTGTTGACGAAATCAAGAAAGTTTCGAAGGCTTTCGTTGTACATCCAAAGAATGTTAATGCAGAGAACTCTACAATACTACCAGTTATGTTGTCCTCAAAACTATTGCCTGAGATGGAGGTAGATGACAATTCGAAACGAGAGCAGTTGCTTCTTGGGATGCACAACATGCCAGTATCTACACAGATTGAGAAATTAAAGACTAGAATTGAAATGATTGGAGCAGCCTGTGAAAGTGCTGAAAAAATATTAGCTGAAACTCGAAAAGCATATTGCTTCGGAATGCGTCAAGGGCCAGCAATAGCTCCAACCTTAGATAAGGCTCAAGCTGCAAAAATTCAGGAGCAAGAAAATTTACTCCGAGCTGCTGTTAACTTTGGGGAAGGATTAAGATTACCTGGTGAGCAACGACAAATTACGCCAACACTTCCGATGCATTTGATAGATGTGCTGAATGCAGGTGATGGAGTGCAGAGTTTTAACGAAACGTCCGGTATGTATGCAAAGAGTACTCCTCTAACGTCAAATAATATTAGTAGTCAAGGAGCAGTGATACAGGGGAAGCAACTTTTGGCTAACAGATTTCGTTTCATTCGAGGAAATTACAGCCCTCCACTTCCAGAAACTGATTAA

Protein sequence

MATIDGAVQNQPQQQQPLPAQQVVFAERLNQAVQQQLNLEALKNRATTLFKAISRILEDFDVYGRTNTTPKWQDILGQYSMVNLELFNIVDEIKKVSKAFVVHPKNVNAENSTILPVMLSSKLLPEMEVDDNSKREQLLLGMHNMPVSTQIEKLKTRIEMIGAACESAEKILAETRKAYCFGMRQGPAIAPTLDKAQAAKIQEQENLLRAAVNFGEGLRLPGEQRQITPTLPMHLIDVLNAGDGVQSFNETSGMYAKSTPLTSNNISSQGAVIQGKQLLANRFRFIRGNYSPPLPETD*

Homology

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BLAST of CSPI03G28140 vs. ExPASy Swiss-Prot
Match: Q4V3C1 (Mediator of RNA polymerase II transcription subunit 8 OS=Arabidopsis thaliana OX=3702 GN=MED8 PE=1 SV=1)

HSP 1 Score: 320.1 bits (819), Expect = 2.7e-86
Identity = 176/293 (60.07%), Postives = 216/293 (73.72%), Query Frame = 0


Query: 8   VQNQPQQQQPLPAQQVVFAERLNQAVQQQLNLEALKNRATTLFKAISRILEDFDVYGRTN 67
           ++ QPQQ  P P      AE+LN  ++++LNLE+LK RA +L KAI+RILEDFD YGRTN
Sbjct: 1   METQPQQPPPPPV-----AEKLNPKLEKELNLESLKTRAVSLAKAIARILEDFDAYGRTN 60

Query: 68  TTPKWQDILGQYSMVNLELFNIVDEIKKVSKAFVVHPKNVNAENSTILPVMLSSKLLPEM 127
           TTPKWQDILGQYSMVNLELFNIV+E+K+VS AFVV PKNVNA N+ ILPVMLSSKLLPEM
Sbjct: 61  TTPKWQDILGQYSMVNLELFNIVEEVKRVSNAFVVLPKNVNAMNAAILPVMLSSKLLPEM 120

Query: 128 EVDDNSKREQLLLGMHNMPVSTQIEKLKTRIEMIGAACESAEKILAETRKAYCFGMRQGP 187
           E DDN+KREQLL G+ ++P+  QIE+LK R++MI AACE+AE++LA+TRKAY FG RQGP
Sbjct: 121 ETDDNAKREQLLQGVQSLPIPMQIERLKARMDMIAAACENAERVLADTRKAYGFGTRQGP 180

Query: 188 AIAPTLDKAQAAKIQEQENLLRAAVNFGEGLRLPGEQRQITPTLPMHLIDVLNAGDGVQS 247
           ++ PT+DK QAAKIQEQE +LR AVN G+G +LP +QRQIT  LP HL DVL   D    
Sbjct: 181 SMLPTMDKGQAAKIQEQEKMLRDAVNDGKGTQLPPDQRQITTALPPHLADVLIIND---- 240

Query: 248 FNETSGMYAKSTPLTSNNISSQGAV-IQGKQLLANRFRFIRG----NYSPPLP 296
               +G  A   P  SNNI++QG + + G Q +        G    N + PLP
Sbjct: 241 ----AGKIA--LPGQSNNINNQGMMQVSGTQFVGRSAASPSGPNFDNTTSPLP 278

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BLAST of CSPI03G28140 vs. ExPASy TrEMBL
Match: A0A0A0L8Z2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G640620 PE=4 SV=1)

HSP 1 Score: 529.3 bits (1362), Expect = 1.1e-146
Identity = 274/274 (100.00%), Postives = 274/274 (100.00%), Query Frame = 0


Query: 1   MATIDGAVQNQPQQQQPLPAQQVVFAERLNQAVQQQLNLEALKNRATTLFKAISRILEDF 60
           MATIDGAVQNQPQQQQPLPAQQVVFAERLNQAVQQQLNLEALKNRATTLFKAISRILEDF
Sbjct: 1   MATIDGAVQNQPQQQQPLPAQQVVFAERLNQAVQQQLNLEALKNRATTLFKAISRILEDF 60

Query: 61  DVYGRTNTTPKWQDILGQYSMVNLELFNIVDEIKKVSKAFVVHPKNVNAENSTILPVMLS 120
           DVYGRTNTTPKWQDILGQYSMVNLELFNIVDEIKKVSKAFVVHPKNVNAENSTILPVMLS
Sbjct: 61  DVYGRTNTTPKWQDILGQYSMVNLELFNIVDEIKKVSKAFVVHPKNVNAENSTILPVMLS 120

Query: 121 SKLLPEMEVDDNSKREQLLLGMHNMPVSTQIEKLKTRIEMIGAACESAEKILAETRKAYC 180
           SKLLPEMEVDDNSKREQLLLGMHNMPVSTQIEKLKTRIEMIGAACESAEKILAETRKAYC
Sbjct: 121 SKLLPEMEVDDNSKREQLLLGMHNMPVSTQIEKLKTRIEMIGAACESAEKILAETRKAYC 180

Query: 181 FGMRQGPAIAPTLDKAQAAKIQEQENLLRAAVNFGEGLRLPGEQRQITPTLPMHLIDVLN 240
           FGMRQGPAIAPTLDKAQAAKIQEQENLLRAAVNFGEGLRLPGEQRQITPTLPMHLIDVLN
Sbjct: 181 FGMRQGPAIAPTLDKAQAAKIQEQENLLRAAVNFGEGLRLPGEQRQITPTLPMHLIDVLN 240

Query: 241 AGDGVQSFNETSGMYAKSTPLTSNNISSQGAVIQ 275
           AGDGVQSFNETSGMYAKSTPLTSNNISSQGAVIQ
Sbjct: 241 AGDGVQSFNETSGMYAKSTPLTSNNISSQGAVIQ 274

[Close]

BLAST of CSPI03G28140 vs. ExPASy TrEMBL
Match: A0A1S3CN44 (mediator of RNA polymerase II transcription subunit 8 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103502790 PE=4 SV=1)

HSP 1 Score: 523.5 bits (1347), Expect = 5.9e-145
Identity = 274/281 (97.51%), Postives = 275/281 (97.86%), Query Frame = 0


Query: 1   MATIDGAVQNQPQQQQPLPAQQVVFAERLNQAVQQQLNLEALKNRATTLFKAISRILEDF 60
           MATIDGAVQNQPQQQQPLPAQQVVFAERLNQAVQQQLNLEALKNRATTLFKAISRILEDF
Sbjct: 1   MATIDGAVQNQPQQQQPLPAQQVVFAERLNQAVQQQLNLEALKNRATTLFKAISRILEDF 60

Query: 61  DVYGRTNTTPKWQDILGQYSMVNLELFNIVDEIKKVSKAFVVHPKNVNAENSTILPVMLS 120
           DVYGRTNTTPKWQDILGQYSMVNLELFNIVDEIKKVSKAFVVHPKNVNAENSTILPVMLS
Sbjct: 61  DVYGRTNTTPKWQDILGQYSMVNLELFNIVDEIKKVSKAFVVHPKNVNAENSTILPVMLS 120

Query: 121 SKLLPEMEVDDNSKREQLLLGMHNMPVSTQIEKLKTRIEMIGAACESAEKILAETRKAYC 180
           SKLLPEMEVDDNSKREQLLLGMHNMPVSTQIEKLKTRIEMIGAACESAEKILAETRKAYC
Sbjct: 121 SKLLPEMEVDDNSKREQLLLGMHNMPVSTQIEKLKTRIEMIGAACESAEKILAETRKAYC 180

Query: 181 FGMRQGPAIAPTLDKAQAAKIQEQENLLRAAVNFGEGLRLPGEQRQITPTLPMHLIDVLN 240
           FGMRQGPAI PTLDKAQAAKIQEQENLLRAAVNFGEGLRLPGEQRQIT TLPMHLIDVLN
Sbjct: 181 FGMRQGPAITPTLDKAQAAKIQEQENLLRAAVNFGEGLRLPGEQRQITSTLPMHLIDVLN 240

Query: 241 AGDGVQSFNETSGMYAKSTPLTSNNISSQGAVIQ--GKQLL 280
           AGDGVQSFNETSGMYAKSTPLTS NISSQGAVIQ  G QL+
Sbjct: 241 AGDGVQSFNETSGMYAKSTPLTSTNISSQGAVIQPPGSQLV 281

[Close]

BLAST of CSPI03G28140 vs. ExPASy TrEMBL
Match: A0A5A7VLA2 (Mediator of RNA polymerase II transcription subunit 8 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold238G001760 PE=4 SV=1)

HSP 1 Score: 523.1 bits (1346), Expect = 7.7e-145
Identity = 274/282 (97.16%), Postives = 275/282 (97.52%), Query Frame = 0


Query: 1   MATIDGAVQNQPQQQQPLPAQQVVFAERLNQAVQQQLNLEALKNRATTLFKAISRILEDF 60
           MATIDGAVQNQPQQQQPLPAQQVVFAERLNQAVQQQLNLEALKNRATTLFKAISRILEDF
Sbjct: 1   MATIDGAVQNQPQQQQPLPAQQVVFAERLNQAVQQQLNLEALKNRATTLFKAISRILEDF 60

Query: 61  DVYGRTNTTPKWQDILGQYSMVNLELFNIVDEIKKVSKAFVVHPKNVNAENSTILPVMLS 120
           DVYGRTNTTPKWQDILGQYSMVNLELFNIVDEIKKVSKAFVVHPKNVNAENSTILPVMLS
Sbjct: 61  DVYGRTNTTPKWQDILGQYSMVNLELFNIVDEIKKVSKAFVVHPKNVNAENSTILPVMLS 120

Query: 121 SKLLPEMEVDDNSKREQLLLGMHNMPVSTQIEKLKTRIEMIGAACESAEKILAETRKAYC 180
           SKLLPEMEVDDNSKREQLLLGMHNMPVSTQIEKLKTRIEMIGAACESAEKILAETRKAYC
Sbjct: 121 SKLLPEMEVDDNSKREQLLLGMHNMPVSTQIEKLKTRIEMIGAACESAEKILAETRKAYC 180

Query: 181 FGMRQGPAIAPTLDKAQAAKIQEQENLLRAAVNFGEGLRLPGEQRQITPTLPMHLIDVLN 240
           FGMRQGPAI PTLDKAQAAKIQEQENLLRAAVNFGEGLRLPGEQRQIT TLPMHLIDVLN
Sbjct: 181 FGMRQGPAITPTLDKAQAAKIQEQENLLRAAVNFGEGLRLPGEQRQITSTLPMHLIDVLN 240

Query: 241 AGDGVQSFNETSGMYAKSTPLTSNNISSQGAVIQ---GKQLL 280
           AGDGVQSFNETSGMYAKSTPLTS NISSQGAVIQ   G QL+
Sbjct: 241 AGDGVQSFNETSGMYAKSTPLTSTNISSQGAVIQQPPGSQLV 282

[Close]

BLAST of CSPI03G28140 vs. ExPASy TrEMBL
Match: A0A1S3CPL4 (mediator of RNA polymerase II transcription subunit 8 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103502790 PE=4 SV=1)

HSP 1 Score: 523.1 bits (1346), Expect = 7.7e-145
Identity = 274/282 (97.16%), Postives = 275/282 (97.52%), Query Frame = 0


Query: 1   MATIDGAVQNQPQQQQPLPAQQVVFAERLNQAVQQQLNLEALKNRATTLFKAISRILEDF 60
           MATIDGAVQNQPQQQQPLPAQQVVFAERLNQAVQQQLNLEALKNRATTLFKAISRILEDF
Sbjct: 1   MATIDGAVQNQPQQQQPLPAQQVVFAERLNQAVQQQLNLEALKNRATTLFKAISRILEDF 60

Query: 61  DVYGRTNTTPKWQDILGQYSMVNLELFNIVDEIKKVSKAFVVHPKNVNAENSTILPVMLS 120
           DVYGRTNTTPKWQDILGQYSMVNLELFNIVDEIKKVSKAFVVHPKNVNAENSTILPVMLS
Sbjct: 61  DVYGRTNTTPKWQDILGQYSMVNLELFNIVDEIKKVSKAFVVHPKNVNAENSTILPVMLS 120

Query: 121 SKLLPEMEVDDNSKREQLLLGMHNMPVSTQIEKLKTRIEMIGAACESAEKILAETRKAYC 180
           SKLLPEMEVDDNSKREQLLLGMHNMPVSTQIEKLKTRIEMIGAACESAEKILAETRKAYC
Sbjct: 121 SKLLPEMEVDDNSKREQLLLGMHNMPVSTQIEKLKTRIEMIGAACESAEKILAETRKAYC 180

Query: 181 FGMRQGPAIAPTLDKAQAAKIQEQENLLRAAVNFGEGLRLPGEQRQITPTLPMHLIDVLN 240
           FGMRQGPAI PTLDKAQAAKIQEQENLLRAAVNFGEGLRLPGEQRQIT TLPMHLIDVLN
Sbjct: 181 FGMRQGPAITPTLDKAQAAKIQEQENLLRAAVNFGEGLRLPGEQRQITSTLPMHLIDVLN 240

Query: 241 AGDGVQSFNETSGMYAKSTPLTSNNISSQGAVIQ---GKQLL 280
           AGDGVQSFNETSGMYAKSTPLTS NISSQGAVIQ   G QL+
Sbjct: 241 AGDGVQSFNETSGMYAKSTPLTSTNISSQGAVIQQPPGSQLV 282

[Close]

BLAST of CSPI03G28140 vs. ExPASy TrEMBL
Match: A0A6J1IWU9 (mediator of RNA polymerase II transcription subunit 8 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111479910 PE=4 SV=1)

HSP 1 Score: 496.1 bits (1276), Expect = 1.0e-136
Identity = 260/302 (86.09%), Postives = 274/302 (90.73%), Query Frame = 0


Query: 1   MATIDGAVQNQPQQQQPLPAQQVVFAERLNQAVQQQLNLEALKNRATTLFKAISRILEDF 60
           MAT+DG VQNQPQQQQPL A QVV AERLNQAVQQQLNLEA+K RATTLFKAISRILEDF
Sbjct: 1   MATMDGVVQNQPQQQQPLLAPQVVVAERLNQAVQQQLNLEAVKTRATTLFKAISRILEDF 60

Query: 61  DVYGRTNTTPKWQDILGQYSMVNLELFNIVDEIKKVSKAFVVHPKNVNAENSTILPVMLS 120
           DVYGRTNTTPKWQDILGQYSMVNLELFNIVDEIKKVSKAFVVHPKNVNAENSTILPVMLS
Sbjct: 61  DVYGRTNTTPKWQDILGQYSMVNLELFNIVDEIKKVSKAFVVHPKNVNAENSTILPVMLS 120

Query: 121 SKLLPEMEVDDNSKREQLLLGMHNMPVSTQIEKLKTRIEMIGAACESAEKILAETRKAYC 180
           SKLLPEMEV+DNSKR+QLLLGM NMP+STQIEKLKTRIEMIGAACESAEKILAETRKAYC
Sbjct: 121 SKLLPEMEVEDNSKRDQLLLGMQNMPISTQIEKLKTRIEMIGAACESAEKILAETRKAYC 180

Query: 181 FGMRQGPAIAPTLDKAQAAKIQEQENLLRAAVNFGEGLRLPGEQRQITPTLPMHLIDVLN 240
           FGMRQGPAIAPTLDKAQAAKIQEQENLLRAAVNFGEGLRLP +QRQITPTLPMHL+DVLN
Sbjct: 181 FGMRQGPAIAPTLDKAQAAKIQEQENLLRAAVNFGEGLRLPIDQRQITPTLPMHLVDVLN 240

Query: 241 AGDGVQSFNETSGMYAKSTPLTSNNISSQGAVIQ--GKQLLANRFRFIRG-----NYSPP 296
            GDGVQSFNETSGMYAK+TPLTS+NI   G+++Q  G QL+        G     N +PP
Sbjct: 241 VGDGVQSFNETSGMYAKNTPLTSHNIGGPGSLLQPPGSQLVGRSAASPSGATSFDNTTPP 300

[Close]

BLAST of CSPI03G28140 vs. NCBI nr
Match: XP_004144418.1 (mediator of RNA polymerase II transcription subunit 8 isoform X2 [Cucumis sativus])

HSP 1 Score: 530.4 bits (1365), Expect = 1.0e-146
Identity = 277/281 (98.58%), Postives = 278/281 (98.93%), Query Frame = 0


Query: 1   MATIDGAVQNQPQQQQPLPAQQVVFAERLNQAVQQQLNLEALKNRATTLFKAISRILEDF 60
           MATIDGAVQNQPQQQQPLPAQQVVFAERLNQAVQQQLNLEALKNRATTLFKAISRILEDF
Sbjct: 1   MATIDGAVQNQPQQQQPLPAQQVVFAERLNQAVQQQLNLEALKNRATTLFKAISRILEDF 60

Query: 61  DVYGRTNTTPKWQDILGQYSMVNLELFNIVDEIKKVSKAFVVHPKNVNAENSTILPVMLS 120
           DVYGRTNTTPKWQDILGQYSMVNLELFNIVDEIKKVSKAFVVHPKNVNAENSTILPVMLS
Sbjct: 61  DVYGRTNTTPKWQDILGQYSMVNLELFNIVDEIKKVSKAFVVHPKNVNAENSTILPVMLS 120

Query: 121 SKLLPEMEVDDNSKREQLLLGMHNMPVSTQIEKLKTRIEMIGAACESAEKILAETRKAYC 180
           SKLLPEMEVDDNSKREQLLLGMHNMPVSTQIEKLKTRIEMIGAACESAEKILAETRKAYC
Sbjct: 121 SKLLPEMEVDDNSKREQLLLGMHNMPVSTQIEKLKTRIEMIGAACESAEKILAETRKAYC 180

Query: 181 FGMRQGPAIAPTLDKAQAAKIQEQENLLRAAVNFGEGLRLPGEQRQITPTLPMHLIDVLN 240
           FGMRQGPAIAPTLDKAQAAKIQEQENLLRAAVNFGEGLRLPGEQRQITPTLPMHLIDVLN
Sbjct: 181 FGMRQGPAIAPTLDKAQAAKIQEQENLLRAAVNFGEGLRLPGEQRQITPTLPMHLIDVLN 240

Query: 241 AGDGVQSFNETSGMYAKSTPLTSNNISSQGAVIQ--GKQLL 280
           AGDGVQSFNETSGMYAKSTPLTSNNISSQGAVIQ  G QL+
Sbjct: 241 AGDGVQSFNETSGMYAKSTPLTSNNISSQGAVIQPPGSQLV 281

[Close]

BLAST of CSPI03G28140 vs. NCBI nr
Match: XP_011651696.1 (mediator of RNA polymerase II transcription subunit 8 isoform X1 [Cucumis sativus] >KAE8650872.1 hypothetical protein Csa_001556 [Cucumis sativus])

HSP 1 Score: 530.0 bits (1364), Expect = 1.3e-146
Identity = 277/282 (98.23%), Postives = 278/282 (98.58%), Query Frame = 0


Query: 1   MATIDGAVQNQPQQQQPLPAQQVVFAERLNQAVQQQLNLEALKNRATTLFKAISRILEDF 60
           MATIDGAVQNQPQQQQPLPAQQVVFAERLNQAVQQQLNLEALKNRATTLFKAISRILEDF
Sbjct: 1   MATIDGAVQNQPQQQQPLPAQQVVFAERLNQAVQQQLNLEALKNRATTLFKAISRILEDF 60

Query: 61  DVYGRTNTTPKWQDILGQYSMVNLELFNIVDEIKKVSKAFVVHPKNVNAENSTILPVMLS 120
           DVYGRTNTTPKWQDILGQYSMVNLELFNIVDEIKKVSKAFVVHPKNVNAENSTILPVMLS
Sbjct: 61  DVYGRTNTTPKWQDILGQYSMVNLELFNIVDEIKKVSKAFVVHPKNVNAENSTILPVMLS 120

Query: 121 SKLLPEMEVDDNSKREQLLLGMHNMPVSTQIEKLKTRIEMIGAACESAEKILAETRKAYC 180
           SKLLPEMEVDDNSKREQLLLGMHNMPVSTQIEKLKTRIEMIGAACESAEKILAETRKAYC
Sbjct: 121 SKLLPEMEVDDNSKREQLLLGMHNMPVSTQIEKLKTRIEMIGAACESAEKILAETRKAYC 180

Query: 181 FGMRQGPAIAPTLDKAQAAKIQEQENLLRAAVNFGEGLRLPGEQRQITPTLPMHLIDVLN 240
           FGMRQGPAIAPTLDKAQAAKIQEQENLLRAAVNFGEGLRLPGEQRQITPTLPMHLIDVLN
Sbjct: 181 FGMRQGPAIAPTLDKAQAAKIQEQENLLRAAVNFGEGLRLPGEQRQITPTLPMHLIDVLN 240

Query: 241 AGDGVQSFNETSGMYAKSTPLTSNNISSQGAVIQ---GKQLL 280
           AGDGVQSFNETSGMYAKSTPLTSNNISSQGAVIQ   G QL+
Sbjct: 241 AGDGVQSFNETSGMYAKSTPLTSNNISSQGAVIQQPPGSQLV 282

[Close]

BLAST of CSPI03G28140 vs. NCBI nr
Match: XP_008465098.1 (PREDICTED: mediator of RNA polymerase II transcription subunit 8 isoform X2 [Cucumis melo])

HSP 1 Score: 523.5 bits (1347), Expect = 1.2e-144
Identity = 274/281 (97.51%), Postives = 275/281 (97.86%), Query Frame = 0


Query: 1   MATIDGAVQNQPQQQQPLPAQQVVFAERLNQAVQQQLNLEALKNRATTLFKAISRILEDF 60
           MATIDGAVQNQPQQQQPLPAQQVVFAERLNQAVQQQLNLEALKNRATTLFKAISRILEDF
Sbjct: 1   MATIDGAVQNQPQQQQPLPAQQVVFAERLNQAVQQQLNLEALKNRATTLFKAISRILEDF 60

Query: 61  DVYGRTNTTPKWQDILGQYSMVNLELFNIVDEIKKVSKAFVVHPKNVNAENSTILPVMLS 120
           DVYGRTNTTPKWQDILGQYSMVNLELFNIVDEIKKVSKAFVVHPKNVNAENSTILPVMLS
Sbjct: 61  DVYGRTNTTPKWQDILGQYSMVNLELFNIVDEIKKVSKAFVVHPKNVNAENSTILPVMLS 120

Query: 121 SKLLPEMEVDDNSKREQLLLGMHNMPVSTQIEKLKTRIEMIGAACESAEKILAETRKAYC 180
           SKLLPEMEVDDNSKREQLLLGMHNMPVSTQIEKLKTRIEMIGAACESAEKILAETRKAYC
Sbjct: 121 SKLLPEMEVDDNSKREQLLLGMHNMPVSTQIEKLKTRIEMIGAACESAEKILAETRKAYC 180

Query: 181 FGMRQGPAIAPTLDKAQAAKIQEQENLLRAAVNFGEGLRLPGEQRQITPTLPMHLIDVLN 240
           FGMRQGPAI PTLDKAQAAKIQEQENLLRAAVNFGEGLRLPGEQRQIT TLPMHLIDVLN
Sbjct: 181 FGMRQGPAITPTLDKAQAAKIQEQENLLRAAVNFGEGLRLPGEQRQITSTLPMHLIDVLN 240

Query: 241 AGDGVQSFNETSGMYAKSTPLTSNNISSQGAVIQ--GKQLL 280
           AGDGVQSFNETSGMYAKSTPLTS NISSQGAVIQ  G QL+
Sbjct: 241 AGDGVQSFNETSGMYAKSTPLTSTNISSQGAVIQPPGSQLV 281

[Close]

BLAST of CSPI03G28140 vs. NCBI nr
Match: XP_008465097.1 (PREDICTED: mediator of RNA polymerase II transcription subunit 8 isoform X1 [Cucumis melo])

HSP 1 Score: 523.1 bits (1346), Expect = 1.6e-144
Identity = 274/282 (97.16%), Postives = 275/282 (97.52%), Query Frame = 0


Query: 1   MATIDGAVQNQPQQQQPLPAQQVVFAERLNQAVQQQLNLEALKNRATTLFKAISRILEDF 60
           MATIDGAVQNQPQQQQPLPAQQVVFAERLNQAVQQQLNLEALKNRATTLFKAISRILEDF
Sbjct: 1   MATIDGAVQNQPQQQQPLPAQQVVFAERLNQAVQQQLNLEALKNRATTLFKAISRILEDF 60

Query: 61  DVYGRTNTTPKWQDILGQYSMVNLELFNIVDEIKKVSKAFVVHPKNVNAENSTILPVMLS 120
           DVYGRTNTTPKWQDILGQYSMVNLELFNIVDEIKKVSKAFVVHPKNVNAENSTILPVMLS
Sbjct: 61  DVYGRTNTTPKWQDILGQYSMVNLELFNIVDEIKKVSKAFVVHPKNVNAENSTILPVMLS 120

Query: 121 SKLLPEMEVDDNSKREQLLLGMHNMPVSTQIEKLKTRIEMIGAACESAEKILAETRKAYC 180
           SKLLPEMEVDDNSKREQLLLGMHNMPVSTQIEKLKTRIEMIGAACESAEKILAETRKAYC
Sbjct: 121 SKLLPEMEVDDNSKREQLLLGMHNMPVSTQIEKLKTRIEMIGAACESAEKILAETRKAYC 180

Query: 181 FGMRQGPAIAPTLDKAQAAKIQEQENLLRAAVNFGEGLRLPGEQRQITPTLPMHLIDVLN 240
           FGMRQGPAI PTLDKAQAAKIQEQENLLRAAVNFGEGLRLPGEQRQIT TLPMHLIDVLN
Sbjct: 181 FGMRQGPAITPTLDKAQAAKIQEQENLLRAAVNFGEGLRLPGEQRQITSTLPMHLIDVLN 240

Query: 241 AGDGVQSFNETSGMYAKSTPLTSNNISSQGAVIQ---GKQLL 280
           AGDGVQSFNETSGMYAKSTPLTS NISSQGAVIQ   G QL+
Sbjct: 241 AGDGVQSFNETSGMYAKSTPLTSTNISSQGAVIQQPPGSQLV 282

[Close]

BLAST of CSPI03G28140 vs. NCBI nr
Match: KAA0068194.1 (mediator of RNA polymerase II transcription subunit 8 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 523.1 bits (1346), Expect = 1.6e-144
Identity = 274/282 (97.16%), Postives = 275/282 (97.52%), Query Frame = 0


Query: 1   MATIDGAVQNQPQQQQPLPAQQVVFAERLNQAVQQQLNLEALKNRATTLFKAISRILEDF 60
           MATIDGAVQNQPQQQQPLPAQQVVFAERLNQAVQQQLNLEALKNRATTLFKAISRILEDF
Sbjct: 1   MATIDGAVQNQPQQQQPLPAQQVVFAERLNQAVQQQLNLEALKNRATTLFKAISRILEDF 60

Query: 61  DVYGRTNTTPKWQDILGQYSMVNLELFNIVDEIKKVSKAFVVHPKNVNAENSTILPVMLS 120
           DVYGRTNTTPKWQDILGQYSMVNLELFNIVDEIKKVSKAFVVHPKNVNAENSTILPVMLS
Sbjct: 61  DVYGRTNTTPKWQDILGQYSMVNLELFNIVDEIKKVSKAFVVHPKNVNAENSTILPVMLS 120

Query: 121 SKLLPEMEVDDNSKREQLLLGMHNMPVSTQIEKLKTRIEMIGAACESAEKILAETRKAYC 180
           SKLLPEMEVDDNSKREQLLLGMHNMPVSTQIEKLKTRIEMIGAACESAEKILAETRKAYC
Sbjct: 121 SKLLPEMEVDDNSKREQLLLGMHNMPVSTQIEKLKTRIEMIGAACESAEKILAETRKAYC 180

Query: 181 FGMRQGPAIAPTLDKAQAAKIQEQENLLRAAVNFGEGLRLPGEQRQITPTLPMHLIDVLN 240
           FGMRQGPAI PTLDKAQAAKIQEQENLLRAAVNFGEGLRLPGEQRQIT TLPMHLIDVLN
Sbjct: 181 FGMRQGPAITPTLDKAQAAKIQEQENLLRAAVNFGEGLRLPGEQRQITSTLPMHLIDVLN 240

Query: 241 AGDGVQSFNETSGMYAKSTPLTSNNISSQGAVIQ---GKQLL 280
           AGDGVQSFNETSGMYAKSTPLTS NISSQGAVIQ   G QL+
Sbjct: 241 AGDGVQSFNETSGMYAKSTPLTSTNISSQGAVIQQPPGSQLV 282

[Close]

BLAST of CSPI03G28140 vs. TAIR 10
Match: AT2G03070.1 (mediator subunit 8 )

HSP 1 Score: 320.1 bits (819), Expect = 1.9e-87
Identity = 176/293 (60.07%), Postives = 216/293 (73.72%), Query Frame = 0


Query: 8   VQNQPQQQQPLPAQQVVFAERLNQAVQQQLNLEALKNRATTLFKAISRILEDFDVYGRTN 67
           ++ QPQQ  P P      AE+LN  ++++LNLE+LK RA +L KAI+RILEDFD YGRTN
Sbjct: 1   METQPQQPPPPPV-----AEKLNPKLEKELNLESLKTRAVSLAKAIARILEDFDAYGRTN 60

Query: 68  TTPKWQDILGQYSMVNLELFNIVDEIKKVSKAFVVHPKNVNAENSTILPVMLSSKLLPEM 127
           TTPKWQDILGQYSMVNLELFNIV+E+K+VS AFVV PKNVNA N+ ILPVMLSSKLLPEM
Sbjct: 61  TTPKWQDILGQYSMVNLELFNIVEEVKRVSNAFVVLPKNVNAMNAAILPVMLSSKLLPEM 120

Query: 128 EVDDNSKREQLLLGMHNMPVSTQIEKLKTRIEMIGAACESAEKILAETRKAYCFGMRQGP 187
           E DDN+KREQLL G+ ++P+  QIE+LK R++MI AACE+AE++LA+TRKAY FG RQGP
Sbjct: 121 ETDDNAKREQLLQGVQSLPIPMQIERLKARMDMIAAACENAERVLADTRKAYGFGTRQGP 180

Query: 188 AIAPTLDKAQAAKIQEQENLLRAAVNFGEGLRLPGEQRQITPTLPMHLIDVLNAGDGVQS 247
           ++ PT+DK QAAKIQEQE +LR AVN G+G +LP +QRQIT  LP HL DVL   D    
Sbjct: 181 SMLPTMDKGQAAKIQEQEKMLRDAVNDGKGTQLPPDQRQITTALPPHLADVLIIND---- 240

Query: 248 FNETSGMYAKSTPLTSNNISSQGAV-IQGKQLLANRFRFIRG----NYSPPLP 296
               +G  A   P  SNNI++QG + + G Q +        G    N + PLP
Sbjct: 241 ----AGKIA--LPGQSNNINNQGMMQVSGTQFVGRSAASPSGPNFDNTTSPLP 278

The following BLAST results are available for this feature:

BLAST of CSPI03G28140 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q4V3C1	2.7e-86	60.07	Mediator of RNA polymerase II transcription subunit 8 OS=Arabidopsis thaliana OX...	[more]

BLAST of CSPI03G28140 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L8Z2	1.1e-146	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G640620 PE=4 SV=1	[more]
A0A1S3CN44	5.9e-145	97.51	mediator of RNA polymerase II transcription subunit 8 isoform X2 OS=Cucumis melo...	[more]
A0A5A7VLA2	7.7e-145	97.16	Mediator of RNA polymerase II transcription subunit 8 isoform X1 OS=Cucumis melo...	[more]
A0A1S3CPL4	7.7e-145	97.16	mediator of RNA polymerase II transcription subunit 8 isoform X1 OS=Cucumis melo...	[more]
A0A6J1IWU9	1.0e-136	86.09	mediator of RNA polymerase II transcription subunit 8 isoform X2 OS=Cucurbita ma...	[more]

BLAST of CSPI03G28140 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004144418.1	1.0e-146	98.58	mediator of RNA polymerase II transcription subunit 8 isoform X2 [Cucumis sativu...	[more]
XP_011651696.1	1.3e-146	98.23	mediator of RNA polymerase II transcription subunit 8 isoform X1 [Cucumis sativu...	[more]
XP_008465098.1	1.2e-144	97.51	PREDICTED: mediator of RNA polymerase II transcription subunit 8 isoform X2 [Cuc...	[more]
XP_008465097.1	1.6e-144	97.16	PREDICTED: mediator of RNA polymerase II transcription subunit 8 isoform X1 [Cuc...	[more]
KAA0068194.1	1.6e-144	97.16	mediator of RNA polymerase II transcription subunit 8 isoform X1 [Cucumis melo v...	[more]

BLAST of CSPI03G28140 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G03070.1	1.9e-87	60.07	mediator subunit 8	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR038795	Mediator of RNA polymerase II transcription subunit 8, plant	PANTHER	PTHR35552	MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 8	coord: 7..278

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G28140.1	CSPI03G28140.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016592	mediator complex