CSPI03G27620 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI03G27620
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	E3 ubiquitin-protein ligase RNF4-like isoform X1
Location	Chr3: 25172951 .. 25173311 (+)
RNA-Seq Expression	CSPI03G27620
Synteny	CSPI03G27620

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCACCAACTGAGAACCGAGATCAAGGAGAAACATCTCAACTGCGGCTTCAGGATGCACAGGTTAATCAACAACAGCCCGTACAACCTGCAATGATTGACATTGAGGCCATTGATGATGATGTCATTGAATCATCTGCCAGAGCTTTTGCTGAAGTATGTTTCCTGTAGCTTCACTTGAATGTTTGAATACTCACGGTTGTTCTATCCTTATTATATTCTTTTTCTATTGATTCTGATCTGTATTTCATGAATTCCCTTGTTTATCCGTCAGGCTAAAAATAAATCCAGACGAAATGCTAGGAAGAATGTAGTTGACGTGGATGCAGGTTTGTTTATTTCAGCTGGTGGAGCACTATAG

mRNA sequence

ATGCCACCAACTGAGAACCGAGATCAAGGAGAAACATCTCAACTGCGGCTTCAGGATGCACAGGTTAATCAACAACAGCCCGTACAACCTGCAATGATTGACATTGAGGCCATTGATGATGATGTCATTGAATCATCTGCCAGAGCTTTTGCTGAAGCTAAAAATAAATCCAGACGAAATGCTAGGAAGAATGTAGTTGACGTGGATGCAGGTTTGTTTATTTCAGCTGGTGGAGCACTATAG

Coding sequence (CDS)

ATGCCACCAACTGAGAACCGAGATCAAGGAGAAACATCTCAACTGCGGCTTCAGGATGCACAGGTTAATCAACAACAGCCCGTACAACCTGCAATGATTGACATTGAGGCCATTGATGATGATGTCATTGAATCATCTGCCAGAGCTTTTGCTGAAGCTAAAAATAAATCCAGACGAAATGCTAGGAAGAATGTAGTTGACGTGGATGCAGGTTTGTTTATTTCAGCTGGTGGAGCACTATAG

Protein sequence

MPPTENRDQGETSQLRLQDAQVNQQQPVQPAMIDIEAIDDDVIESSARAFAEAKNKSRRNARKNVVDVDAGLFISAGGAL*

Homology

[Close]

BLAST of CSPI03G27620 vs. ExPASy TrEMBL
Match: A0A0A0LBP4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G630250 PE=4 SV=1)

HSP 1 Score: 151.8 bits (382), Expect = 1.3e-33
Identity = 80/80 (100.00%), Postives = 80/80 (100.00%), Query Frame = 0


Query: 1  MPPTENRDQGETSQLRLQDAQVNQQQPVQPAMIDIEAIDDDVIESSARAFAEAKNKSRRN 60
          MPPTENRDQGETSQLRLQDAQVNQQQPVQPAMIDIEAIDDDVIESSARAFAEAKNKSRRN
Sbjct: 1  MPPTENRDQGETSQLRLQDAQVNQQQPVQPAMIDIEAIDDDVIESSARAFAEAKNKSRRN 60

Query: 61 ARKNVVDVDAGLFISAGGAL 81
          ARKNVVDVDAGLFISAGGAL
Sbjct: 61 ARKNVVDVDAGLFISAGGAL 80

[Close]

BLAST of CSPI03G27620 vs. ExPASy TrEMBL
Match: A0A5D3DQU9 (E3 ubiquitin-protein ligase RNF4-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1185G00040 PE=4 SV=1)

HSP 1 Score: 149.1 bits (375), Expect = 8.2e-33
Identity = 78/80 (97.50%), Postives = 79/80 (98.75%), Query Frame = 0


Query: 1   MPPTENRDQGETSQLRLQDAQVNQQQPVQPAMIDIEAIDDDVIESSARAFAEAKNKSRRN 60
           MPPTENRDQGETSQLRLQDAQVNQQQPVQPAMIDIEAIDDDVIESSARAFAEAKNKSRRN
Sbjct: 27  MPPTENRDQGETSQLRLQDAQVNQQQPVQPAMIDIEAIDDDVIESSARAFAEAKNKSRRN 86

Query: 61  ARKNVVDVDAGLFISAGGAL 81
           ARKNVVDVDAG+FI AGGAL
Sbjct: 87  ARKNVVDVDAGMFIPAGGAL 106

[Close]

BLAST of CSPI03G27620 vs. ExPASy TrEMBL
Match: A0A1S3CCB4 (E3 ubiquitin-protein ligase RNF4-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103499213 PE=4 SV=1)

HSP 1 Score: 132.9 bits (333), Expect = 6.1e-28
Identity = 70/70 (100.00%), Postives = 70/70 (100.00%), Query Frame = 0


Query: 1  MPPTENRDQGETSQLRLQDAQVNQQQPVQPAMIDIEAIDDDVIESSARAFAEAKNKSRRN 60
          MPPTENRDQGETSQLRLQDAQVNQQQPVQPAMIDIEAIDDDVIESSARAFAEAKNKSRRN
Sbjct: 27 MPPTENRDQGETSQLRLQDAQVNQQQPVQPAMIDIEAIDDDVIESSARAFAEAKNKSRRN 86

Query: 61 ARKNVVDVDA 71
          ARKNVVDVDA
Sbjct: 87 ARKNVVDVDA 96

[Close]

BLAST of CSPI03G27620 vs. ExPASy TrEMBL
Match: A0A1S3CBW2 (E3 ubiquitin-protein ligase RNF4-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103499213 PE=4 SV=1)

HSP 1 Score: 132.9 bits (333), Expect = 6.1e-28
Identity = 70/70 (100.00%), Postives = 70/70 (100.00%), Query Frame = 0


Query: 1  MPPTENRDQGETSQLRLQDAQVNQQQPVQPAMIDIEAIDDDVIESSARAFAEAKNKSRRN 60
          MPPTENRDQGETSQLRLQDAQVNQQQPVQPAMIDIEAIDDDVIESSARAFAEAKNKSRRN
Sbjct: 27 MPPTENRDQGETSQLRLQDAQVNQQQPVQPAMIDIEAIDDDVIESSARAFAEAKNKSRRN 86

Query: 61 ARKNVVDVDA 71
          ARKNVVDVDA
Sbjct: 87 ARKNVVDVDA 96

[Close]

BLAST of CSPI03G27620 vs. ExPASy TrEMBL
Match: A0A6J1HQI7 (E3 ubiquitin-protein ligase RNF4-like OS=Cucurbita maxima OX=3661 GN=LOC111466888 PE=4 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 1.1e-24
Identity = 64/70 (91.43%), Postives = 66/70 (94.29%), Query Frame = 0


Query: 1  MPPTENRDQGETSQLRLQDAQVNQQQPVQPAMIDIEAIDDDVIESSARAFAEAKNKSRRN 60
          MPPTENRDQGETSQ   Q+AQVNQQQ VQPAMIDIEAIDDDVIESSARAFAEAKNKSRRN
Sbjct: 27 MPPTENRDQGETSQFGFQEAQVNQQQSVQPAMIDIEAIDDDVIESSARAFAEAKNKSRRN 86

Query: 61 ARKNVVDVDA 71
          ARKN+VDVDA
Sbjct: 87 ARKNIVDVDA 96

[Close]

BLAST of CSPI03G27620 vs. NCBI nr
Match: KAA0068067.1 (E3 ubiquitin-protein ligase RNF4-like isoform X1 [Cucumis melo var. makuwa] >TYK26051.1 E3 ubiquitin-protein ligase RNF4-like isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 149.1 bits (375), Expect = 1.7e-32
Identity = 78/80 (97.50%), Postives = 79/80 (98.75%), Query Frame = 0


Query: 1   MPPTENRDQGETSQLRLQDAQVNQQQPVQPAMIDIEAIDDDVIESSARAFAEAKNKSRRN 60
           MPPTENRDQGETSQLRLQDAQVNQQQPVQPAMIDIEAIDDDVIESSARAFAEAKNKSRRN
Sbjct: 27  MPPTENRDQGETSQLRLQDAQVNQQQPVQPAMIDIEAIDDDVIESSARAFAEAKNKSRRN 86

Query: 61  ARKNVVDVDAGLFISAGGAL 81
           ARKNVVDVDAG+FI AGGAL
Sbjct: 87  ARKNVVDVDAGMFIPAGGAL 106

[Close]

BLAST of CSPI03G27620 vs. NCBI nr
Match: XP_008460373.1 (PREDICTED: E3 ubiquitin-protein ligase RNF4-like isoform X2 [Cucumis melo])

HSP 1 Score: 132.9 bits (333), Expect = 1.3e-27
Identity = 70/70 (100.00%), Postives = 70/70 (100.00%), Query Frame = 0


Query: 1  MPPTENRDQGETSQLRLQDAQVNQQQPVQPAMIDIEAIDDDVIESSARAFAEAKNKSRRN 60
          MPPTENRDQGETSQLRLQDAQVNQQQPVQPAMIDIEAIDDDVIESSARAFAEAKNKSRRN
Sbjct: 27 MPPTENRDQGETSQLRLQDAQVNQQQPVQPAMIDIEAIDDDVIESSARAFAEAKNKSRRN 86

Query: 61 ARKNVVDVDA 71
          ARKNVVDVDA
Sbjct: 87 ARKNVVDVDA 96

[Close]

BLAST of CSPI03G27620 vs. NCBI nr
Match: XP_008460369.1 (PREDICTED: E3 ubiquitin-protein ligase RNF4-like isoform X1 [Cucumis melo] >XP_008460370.1 PREDICTED: E3 ubiquitin-protein ligase RNF4-like isoform X1 [Cucumis melo] >XP_008460371.1 PREDICTED: E3 ubiquitin-protein ligase RNF4-like isoform X1 [Cucumis melo] >XP_008460372.1 PREDICTED: E3 ubiquitin-protein ligase RNF4-like isoform X1 [Cucumis melo] >XP_016902544.1 PREDICTED: E3 ubiquitin-protein ligase RNF4-like isoform X1 [Cucumis melo])

HSP 1 Score: 132.9 bits (333), Expect = 1.3e-27
Identity = 70/70 (100.00%), Postives = 70/70 (100.00%), Query Frame = 0


Query: 1  MPPTENRDQGETSQLRLQDAQVNQQQPVQPAMIDIEAIDDDVIESSARAFAEAKNKSRRN 60
          MPPTENRDQGETSQLRLQDAQVNQQQPVQPAMIDIEAIDDDVIESSARAFAEAKNKSRRN
Sbjct: 27 MPPTENRDQGETSQLRLQDAQVNQQQPVQPAMIDIEAIDDDVIESSARAFAEAKNKSRRN 86

Query: 61 ARKNVVDVDA 71
          ARKNVVDVDA
Sbjct: 87 ARKNVVDVDA 96

[Close]

BLAST of CSPI03G27620 vs. NCBI nr
Match: XP_004144388.1 (E3 ubiquitin-protein ligase RNF4 [Cucumis sativus] >XP_031738436.1 E3 ubiquitin-protein ligase RNF4 [Cucumis sativus] >KAE8650852.1 hypothetical protein Csa_000772 [Cucumis sativus])

HSP 1 Score: 132.9 bits (333), Expect = 1.3e-27
Identity = 70/70 (100.00%), Postives = 70/70 (100.00%), Query Frame = 0


Query: 1  MPPTENRDQGETSQLRLQDAQVNQQQPVQPAMIDIEAIDDDVIESSARAFAEAKNKSRRN 60
          MPPTENRDQGETSQLRLQDAQVNQQQPVQPAMIDIEAIDDDVIESSARAFAEAKNKSRRN
Sbjct: 27 MPPTENRDQGETSQLRLQDAQVNQQQPVQPAMIDIEAIDDDVIESSARAFAEAKNKSRRN 86

Query: 61 ARKNVVDVDA 71
          ARKNVVDVDA
Sbjct: 87 ARKNVVDVDA 96

[Close]

BLAST of CSPI03G27620 vs. NCBI nr
Match: XP_038888129.1 (E3 ubiquitin-protein ligase RNF4-like [Benincasa hispida] >XP_038888130.1 E3 ubiquitin-protein ligase RNF4-like [Benincasa hispida] >XP_038888131.1 E3 ubiquitin-protein ligase RNF4-like [Benincasa hispida] >XP_038888132.1 E3 ubiquitin-protein ligase RNF4-like [Benincasa hispida] >XP_038888133.1 E3 ubiquitin-protein ligase RNF4-like [Benincasa hispida] >XP_038888134.1 E3 ubiquitin-protein ligase RNF4-like [Benincasa hispida] >XP_038888135.1 E3 ubiquitin-protein ligase RNF4-like [Benincasa hispida])

HSP 1 Score: 125.9 bits (315), Expect = 1.5e-25
Identity = 66/70 (94.29%), Postives = 68/70 (97.14%), Query Frame = 0


Query: 1  MPPTENRDQGETSQLRLQDAQVNQQQPVQPAMIDIEAIDDDVIESSARAFAEAKNKSRRN 60
          MPPTENRDQGETSQLR Q+AQVNQQQ VQPAMIDIEAIDDDVIESSARAFAEAKNKSRRN
Sbjct: 27 MPPTENRDQGETSQLRFQEAQVNQQQHVQPAMIDIEAIDDDVIESSARAFAEAKNKSRRN 86

Query: 61 ARKNVVDVDA 71
          ARKN+VDVDA
Sbjct: 87 ARKNIVDVDA 96

[Close]

BLAST of CSPI03G27620 vs. TAIR 10
Match: AT5G48655.2 (RING/U-box superfamily protein )

HSP 1 Score: 55.5 bits (132), Expect = 2.4e-08
Identity = 34/65 (52.31%), Postives = 47/65 (72.31%), Query Frame = 0


Query: 9  QGETSQLRLQDAQVNQQQPVQPAMIDIEAIDDDVIESSARAFAEAKNKSRRNARKN--VV 68
          +G ++ +R     +   QP  P MID++AI+DDVIESSA AFAEAK+KS RNAR+   +V
Sbjct: 29 EGTSASVRTLTVPITPSQPA-PTMIDVDAIEDDVIESSASAFAEAKSKS-RNARRRPLMV 88

Query: 69 DVDAG 72
          DV++G
Sbjct: 89 DVESG 91

[Close]

BLAST of CSPI03G27620 vs. TAIR 10
Match: AT5G48655.1 (RING/U-box superfamily protein )

HSP 1 Score: 55.5 bits (132), Expect = 2.4e-08
Identity = 34/65 (52.31%), Postives = 47/65 (72.31%), Query Frame = 0


Query: 9  QGETSQLRLQDAQVNQQQPVQPAMIDIEAIDDDVIESSARAFAEAKNKSRRNARKN--VV 68
          +G ++ +R     +   QP  P MID++AI+DDVIESSA AFAEAK+KS RNAR+   +V
Sbjct: 29 EGTSASVRTLTVPITPSQPA-PTMIDVDAIEDDVIESSASAFAEAKSKS-RNARRRPLMV 88

Query: 69 DVDAG 72
          DV++G
Sbjct: 89 DVESG 91

[Close]

BLAST of CSPI03G27620 vs. TAIR 10
Match: AT5G48655.3 (RING/U-box superfamily protein )

HSP 1 Score: 55.5 bits (132), Expect = 2.4e-08
Identity = 34/65 (52.31%), Postives = 47/65 (72.31%), Query Frame = 0


Query: 9  QGETSQLRLQDAQVNQQQPVQPAMIDIEAIDDDVIESSARAFAEAKNKSRRNARKN--VV 68
          +G ++ +R     +   QP  P MID++AI+DDVIESSA AFAEAK+KS RNAR+   +V
Sbjct: 29 EGTSASVRTLTVPITPSQPA-PTMIDVDAIEDDVIESSASAFAEAKSKS-RNARRRPLMV 88

Query: 69 DVDAG 72
          DV++G
Sbjct: 89 DVESG 91

[Close]

BLAST of CSPI03G27620 vs. TAIR 10
Match: AT3G07200.1 (RING/U-box superfamily protein )

HSP 1 Score: 42.0 bits (97), Expect = 2.7e-04
Identity = 33/73 (45.21%), Postives = 41/73 (56.16%), Query Frame = 0


Query: 7  RDQGETSQLRLQDAQVNQQQPVQPAM------IDIEAI--DDDVIESSARAFAEAKNKSR 66
          R  G T      D QV  Q P  P +      I++ AI  DDDV+ES+A AFA+AKNKSR
Sbjct: 14 RRDGVTQNEHQGDQQVADQAPTVPQIVATPPRINVVAIEDDDDVVESTASAFAQAKNKSR 73

Query: 67 RNAR-KNVVDVDA 71
             R   VVDV++
Sbjct: 74 SARRGSQVVDVES 86

[Close]

BLAST of CSPI03G27620 vs. TAIR 10
Match: AT3G07200.2 (RING/U-box superfamily protein )

HSP 1 Score: 42.0 bits (97), Expect = 2.7e-04
Identity = 33/73 (45.21%), Postives = 41/73 (56.16%), Query Frame = 0


Query: 7  RDQGETSQLRLQDAQVNQQQPVQPAM------IDIEAI--DDDVIESSARAFAEAKNKSR 66
          R  G T      D QV  Q P  P +      I++ AI  DDDV+ES+A AFA+AKNKSR
Sbjct: 14 RRDGVTQNEHQGDQQVADQAPTVPQIVATPPRINVVAIEDDDDVVESTASAFAQAKNKSR 73

Query: 67 RNAR-KNVVDVDA 71
             R   VVDV++
Sbjct: 74 SARRGSQVVDVES 86

The following BLAST results are available for this feature:

BLAST of CSPI03G27620 vs.


Match Name	E-value	Identity	Description

BLAST of CSPI03G27620 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LBP4	1.3e-33	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G630250 PE=4 SV=1	[more]
A0A5D3DQU9	8.2e-33	97.50	E3 ubiquitin-protein ligase RNF4-like isoform X1 OS=Cucumis melo var. makuwa OX=...	[more]
A0A1S3CCB4	6.1e-28	100.00	E3 ubiquitin-protein ligase RNF4-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC1...	[more]
A0A1S3CBW2	6.1e-28	100.00	E3 ubiquitin-protein ligase RNF4-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC1...	[more]
A0A6J1HQI7	1.1e-24	91.43	E3 ubiquitin-protein ligase RNF4-like OS=Cucurbita maxima OX=3661 GN=LOC11146688...	[more]

BLAST of CSPI03G27620 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0068067.1	1.7e-32	97.50	E3 ubiquitin-protein ligase RNF4-like isoform X1 [Cucumis melo var. makuwa] >TYK...	[more]
XP_008460373.1	1.3e-27	100.00	PREDICTED: E3 ubiquitin-protein ligase RNF4-like isoform X2 [Cucumis melo]	[more]
XP_008460369.1	1.3e-27	100.00	PREDICTED: E3 ubiquitin-protein ligase RNF4-like isoform X1 [Cucumis melo] >XP_0...	[more]
XP_004144388.1	1.3e-27	100.00	E3 ubiquitin-protein ligase RNF4 [Cucumis sativus] >XP_031738436.1 E3 ubiquitin-...	[more]
XP_038888129.1	1.5e-25	94.29	E3 ubiquitin-protein ligase RNF4-like [Benincasa hispida] >XP_038888130.1 E3 ubi...	[more]

BLAST of CSPI03G27620 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G48655.2	2.4e-08	52.31	RING/U-box superfamily protein	[more]
AT5G48655.1	2.4e-08	52.31	RING/U-box superfamily protein	[more]
AT5G48655.3	2.4e-08	52.31	RING/U-box superfamily protein	[more]
AT3G07200.1	2.7e-04	45.21	RING/U-box superfamily protein	[more]
AT3G07200.2	2.7e-04	45.21	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..27

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G27620.1	CSPI03G27620.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0046872	metal ion binding