CSPI03G27340 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI03G27340
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Descriptionpentatricopeptide repeat-containing protein At2g15820, chloroplastic
LocationChr3: 24941404 .. 24941924 (+)
RNA-Seq ExpressionCSPI03G27340
SyntenyCSPI03G27340
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTTTTCTCCACGTCCATTCCTACCTCTGCATTTTCCACTGTGACCCTTCTCCGTTCTCTCACTCTTTCCCTCTCTCCATACCATCACGACTTTCGTTGTCCCAATCATATAATCCTTACTCTCTTTCTTCCTGCATATTCTGTTAAAGTACCACAACTTCCCCGAATTCGTGCCTTTGCTTCCGGTTCTTTTGTTAAACAGCTGGTGTACGACTAAGATTCCCCGTTCGAATCTGAGAAGCATTTATCATCTCCAAACAGTAATGGGGTGATGGTTTATTCATTTTGAAAATAGTTTTGCATCGGTGGGTTTGCAACATTTGGGAACGCCAGCCGCTCGAAGTCAAGGAGCTTGATGAGTCATCGGAGCAATGGCGAATATCCAGATTGGCTTGGCTTTGTAAGAAATTTCCAGCACGAAAGCCGAGAATAGTGATACGACTGCTTAACGCGCAGAAAAAATGGATGGGTCGGGATGATGCGACCTATCTCACCGTGCATTGTTTGCGTATTCGTTAA

mRNA sequence

ATGGTTTTCTCCACGTCCATTCCTACCTCTGCATTTTCCACTGTGACCCTTCTCCGTTCTCTCACTCTTTCCCTCTCTCCATACCATCACGACTTTCGTTGTCCCAATCATATAATCCTTACTCTCTTTCTTCCTGCATATTCTGTTAAAGTACCACAACTTCCCCGAATTCGTGCCTTTGCTTCCGTTTTGCATCGGTGGGTTTGCAACATTTGGGAACGCCAGCCGCTCGAAGTCAAGGAGCTTGATGAGTCATCGGAGCAATGGCGAATATCCAGATTGGCTTGGCTTTGTAAGAAATTTCCAGCACGAAAGCCGAGAATAGTGATACGACTGCTTAACGCGCAGAAAAAATGGATGGGTCGGGATGATGCGACCTATCTCACCGTGCATTGTTTGCGTATTCGTTAA

Coding sequence (CDS)

ATGGTTTTCTCCACGTCCATTCCTACCTCTGCATTTTCCACTGTGACCCTTCTCCGTTCTCTCACTCTTTCCCTCTCTCCATACCATCACGACTTTCGTTGTCCCAATCATATAATCCTTACTCTCTTTCTTCCTGCATATTCTGTTAAAGTACCACAACTTCCCCGAATTCGTGCCTTTGCTTCCGTTTTGCATCGGTGGGTTTGCAACATTTGGGAACGCCAGCCGCTCGAAGTCAAGGAGCTTGATGAGTCATCGGAGCAATGGCGAATATCCAGATTGGCTTGGCTTTGTAAGAAATTTCCAGCACGAAAGCCGAGAATAGTGATACGACTGCTTAACGCGCAGAAAAAATGGATGGGTCGGGATGATGCGACCTATCTCACCGTGCATTGTTTGCGTATTCGTTAA

Protein sequence

MVFSTSIPTSAFSTVTLLRSLTLSLSPYHHDFRCPNHIILTLFLPAYSVKVPQLPRIRAFASVLHRWVCNIWERQPLEVKELDESSEQWRISRLAWLCKKFPARKPRIVIRLLNAQKKWMGRDDATYLTVHCLRIR*
Homology
BLAST of CSPI03G27340 vs. ExPASy Swiss-Prot
Match: Q9XIL5 (Pentatricopeptide repeat-containing protein At2g15820, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=OTP51 PE=2 SV=3)

HSP 1 Score: 87.4 bits (215), Expect = 1.3e-16
Identity = 36/59 (61.02%), Postives = 49/59 (83.05%), Query Frame = 0

Query: 78  EVKELDESSEQWRISRLAWLCKKFPARKPRIVIRLLNAQKKWMGRDDATYLTVHCLRIR 137
           EV+EL+E  E+WR S+LAWLCK+ P  K   ++RLLNAQKKW+ ++DATY++VHC+RIR
Sbjct: 158 EVRELEELPEEWRRSKLAWLCKEVPTHKAVTLVRLLNAQKKWVRQEDATYISVHCMRIR 216

BLAST of CSPI03G27340 vs. ExPASy Swiss-Prot
Match: Q6ZHJ5 (Pentatricopeptide repeat-containing protein OTP51, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=OTP51 PE=3 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 5.1e-16
Identity = 38/60 (63.33%), Postives = 46/60 (76.67%), Query Frame = 0

Query: 77  LEVKELDESSEQWRISRLAWLCKKFPARKPRIVIRLLNAQKKWMGRDDATYLTVHCLRIR 136
           L V EL+E  EQWR SR+AWLCK+ PA K     R+LNAQ+KW+ +DDATY+ VHCLRIR
Sbjct: 102 LVVPELEELPEQWRRSRIAWLCKELPAYKHSTFTRILNAQRKWITQDDATYVAVHCLRIR 161

BLAST of CSPI03G27340 vs. ExPASy TrEMBL
Match: A0A1S3CPK0 (pentatricopeptide repeat-containing protein At2g15820, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103502781 PE=4 SV=1)

HSP 1 Score: 180.6 bits (457), Expect = 4.3e-42
Identity = 105/172 (61.05%), Postives = 113/172 (65.70%), Query Frame = 0

Query: 1   MVFSTSIPTSAFSTVTLLRSLTLSLSPYHHDFRCPNHIILTLFLPAYSVKVPQLPRIRAF 60
           MVFS SIPTSAFSTVTLLRSLTLSLSPYHH F  PNHII TLF+ +YSVKV QLPRIRAF
Sbjct: 1   MVFSMSIPTSAFSTVTLLRSLTLSLSPYHHYFHYPNHIIPTLFISSYSVKVRQLPRIRAF 60

Query: 61  AS-------VLHRWVCNIWERQ-----------------------------PLEVKELDE 120
           AS       V  R   +  E                                LEVKELDE
Sbjct: 61  ASGSFVKQLVYDRDSPSESEEHLSSPYSNGGDGFHFENGFASVDLKHLGTPALEVKELDE 120

Query: 121 SSEQWRISRLAWLCKKFPARKPRIVIRLLNAQKKWMGRDDATYLTVHCLRIR 137
             EQWR S+LAWLCK+ PA+KP  VIRLLNAQ+KWMG+DDATYLTVHCLRIR
Sbjct: 121 LPEQWRRSKLAWLCKELPAQKPGTVIRLLNAQRKWMGQDDATYLTVHCLRIR 172

BLAST of CSPI03G27340 vs. ExPASy TrEMBL
Match: A0A0A0LBL0 (LAGLIDADG_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G625100 PE=4 SV=1)

HSP 1 Score: 179.1 bits (453), Expect = 1.3e-41
Identity = 105/173 (60.69%), Postives = 110/173 (63.58%), Query Frame = 0

Query: 1   MVFSTSIPTSAFSTVTLLRSLTLSLSPYHHDFRCPNHIILTLFLPAYSVKV-PQLPRIRA 60
           MVFS SIPTSAFSTVT LRSLTLSLSPYHH F CPNHII TLFLPAYSVKV  QLPRIRA
Sbjct: 1   MVFSMSIPTSAFSTVTRLRSLTLSLSPYHHYFHCPNHIIPTLFLPAYSVKVRRQLPRIRA 60

Query: 61  FASVLHRWVCNIWERQP------------------------------------LEVKELD 120
           FAS             P                                    LEVKELD
Sbjct: 61  FASGSFVKQLVYDHDSPSESEEHLSSSFSNGGDGFHFENGFASVDLKHLGTPVLEVKELD 120

Query: 121 ESSEQWRISRLAWLCKKFPARKPRIVIRLLNAQKKWMGRDDATYLTVHCLRIR 137
           E  EQWR S++AWLCK+ PA+KP  VIRLLNAQKKWMG+DDATYL VHCLRIR
Sbjct: 121 ELPEQWRRSKVAWLCKELPAQKPGTVIRLLNAQKKWMGQDDATYLIVHCLRIR 173

BLAST of CSPI03G27340 vs. ExPASy TrEMBL
Match: A0A6J1GB98 (pentatricopeptide repeat-containing protein At2g15820, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111452602 PE=4 SV=1)

HSP 1 Score: 142.5 bits (358), Expect = 1.3e-30
Identity = 84/162 (51.85%), Postives = 98/162 (60.49%), Query Frame = 0

Query: 6   SIPTSAFSTVTLLRSLTLSLSPYHHDFRCPNHIILTLFLPAYSVK-VPQLPRIRAFAS-- 65
           SI TSAF+TVTLLRSLTL  S  HH FRC N++I +L +P YS K   QLPRI AFAS  
Sbjct: 2   SIRTSAFATVTLLRSLTLPFSQCHHHFRCRNYVIRSLCIPTYSAKGRRQLPRIPAFASSS 61

Query: 66  -------------VLHRWVCNIWE---------------RQPLEVKELDESSEQWRISRL 125
                             +C+ +                   LEVKELDE  EQWR S+L
Sbjct: 62  SVEALVYDRDSPAESEEPLCSPYSTGAEGFASADLKHLGAPALEVKELDELPEQWRRSKL 121

Query: 126 AWLCKKFPARKPRIVIRLLNAQKKWMGRDDATYLTVHCLRIR 137
           AWLCK+ PA+KP  +IRLLNAQ+KWM +DDA YL VHCLRIR
Sbjct: 122 AWLCKELPAQKPGTLIRLLNAQRKWMKQDDAAYLIVHCLRIR 163

BLAST of CSPI03G27340 vs. ExPASy TrEMBL
Match: A0A6J1KB64 (pentatricopeptide repeat-containing protein At2g15820, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111493350 PE=4 SV=1)

HSP 1 Score: 139.0 bits (349), Expect = 1.4e-29
Identity = 83/162 (51.23%), Postives = 97/162 (59.88%), Query Frame = 0

Query: 6   SIPTSAFSTVTLLRSLTLSLSPYHHDFRCPNHIILTLFLPAYSVK-VPQLPRIRAFAS-- 65
           SI TSAF+TVTLLRSLTL  S  H+ FRC N++I +L +P YS K   QLPRI AFAS  
Sbjct: 2   SIRTSAFATVTLLRSLTLPFSQCHNHFRCWNYVIRSLSIPTYSAKGRRQLPRIPAFASSS 61

Query: 66  -------------VLHRWVCNIWER---------------QPLEVKELDESSEQWRISRL 125
                             +C+ +                   LEVKELDE  EQWR S+L
Sbjct: 62  SVEALVYDRDSPAESEEPLCSPYSNGAEEFASADLKHLGAPALEVKELDELPEQWRRSKL 121

Query: 126 AWLCKKFPARKPRIVIRLLNAQKKWMGRDDATYLTVHCLRIR 137
           AWLCK+ PA KP  +IRLLNAQ+KWM +DDA YL VHCLRIR
Sbjct: 122 AWLCKELPAHKPGTLIRLLNAQRKWMKQDDAAYLIVHCLRIR 163

BLAST of CSPI03G27340 vs. ExPASy TrEMBL
Match: A0A6J1DXY9 (pentatricopeptide repeat-containing protein At2g15820, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111025188 PE=4 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 2.1e-25
Identity = 73/158 (46.20%), Postives = 87/158 (55.06%), Query Frame = 0

Query: 16  TLLRSLTLSLSPYHHDFRCPNHIILTLFLPAYSVK-VPQLPRIRAFASV----------- 75
           TL RSLT SL  +H  FRC N+I+ TLF+  +S K  P+LPRI AFAS            
Sbjct: 3   TLFRSLTHSLPSHHRHFRCHNYIVRTLFIQTFSAKRRPKLPRITAFASSSLVAQLLYDRD 62

Query: 76  ----LHRWVCNIWE---------------------RQPLEVKELDESSEQWRISRLAWLC 135
                    C+ +                         LEVKELDE  EQWR S+LAWLC
Sbjct: 63  SPSDSEEHSCSPYSNGADGFHFENSFASADLKHLGNPALEVKELDELPEQWRRSKLAWLC 122

Query: 136 KKFPARKPRIVIRLLNAQKKWMGRDDATYLTVHCLRIR 137
           K+ PA KP  ++RLLNAQ+KWM +DDA YL VHCLRIR
Sbjct: 123 KELPAHKPGTLVRLLNAQRKWMRQDDAAYLIVHCLRIR 160

BLAST of CSPI03G27340 vs. NCBI nr
Match: XP_008465080.1 (PREDICTED: pentatricopeptide repeat-containing protein At2g15820, chloroplastic [Cucumis melo])

HSP 1 Score: 180.6 bits (457), Expect = 8.9e-42
Identity = 105/172 (61.05%), Postives = 113/172 (65.70%), Query Frame = 0

Query: 1   MVFSTSIPTSAFSTVTLLRSLTLSLSPYHHDFRCPNHIILTLFLPAYSVKVPQLPRIRAF 60
           MVFS SIPTSAFSTVTLLRSLTLSLSPYHH F  PNHII TLF+ +YSVKV QLPRIRAF
Sbjct: 1   MVFSMSIPTSAFSTVTLLRSLTLSLSPYHHYFHYPNHIIPTLFISSYSVKVRQLPRIRAF 60

Query: 61  AS-------VLHRWVCNIWERQ-----------------------------PLEVKELDE 120
           AS       V  R   +  E                                LEVKELDE
Sbjct: 61  ASGSFVKQLVYDRDSPSESEEHLSSPYSNGGDGFHFENGFASVDLKHLGTPALEVKELDE 120

Query: 121 SSEQWRISRLAWLCKKFPARKPRIVIRLLNAQKKWMGRDDATYLTVHCLRIR 137
             EQWR S+LAWLCK+ PA+KP  VIRLLNAQ+KWMG+DDATYLTVHCLRIR
Sbjct: 121 LPEQWRRSKLAWLCKELPAQKPGTVIRLLNAQRKWMGQDDATYLTVHCLRIR 172

BLAST of CSPI03G27340 vs. NCBI nr
Match: XP_004152074.2 (pentatricopeptide repeat-containing protein At2g15820, chloroplastic [Cucumis sativus] >KGN58344.1 hypothetical protein Csa_017589 [Cucumis sativus])

HSP 1 Score: 179.1 bits (453), Expect = 2.6e-41
Identity = 105/173 (60.69%), Postives = 110/173 (63.58%), Query Frame = 0

Query: 1   MVFSTSIPTSAFSTVTLLRSLTLSLSPYHHDFRCPNHIILTLFLPAYSVKV-PQLPRIRA 60
           MVFS SIPTSAFSTVT LRSLTLSLSPYHH F CPNHII TLFLPAYSVKV  QLPRIRA
Sbjct: 1   MVFSMSIPTSAFSTVTRLRSLTLSLSPYHHYFHCPNHIIPTLFLPAYSVKVRRQLPRIRA 60

Query: 61  FASVLHRWVCNIWERQP------------------------------------LEVKELD 120
           FAS             P                                    LEVKELD
Sbjct: 61  FASGSFVKQLVYDHDSPSESEEHLSSSFSNGGDGFHFENGFASVDLKHLGTPVLEVKELD 120

Query: 121 ESSEQWRISRLAWLCKKFPARKPRIVIRLLNAQKKWMGRDDATYLTVHCLRIR 137
           E  EQWR S++AWLCK+ PA+KP  VIRLLNAQKKWMG+DDATYL VHCLRIR
Sbjct: 121 ELPEQWRRSKVAWLCKELPAQKPGTVIRLLNAQKKWMGQDDATYLIVHCLRIR 173

BLAST of CSPI03G27340 vs. NCBI nr
Match: XP_038887990.1 (pentatricopeptide repeat-containing protein At2g15820, chloroplastic [Benincasa hispida])

HSP 1 Score: 160.2 bits (404), Expect = 1.2e-35
Identity = 91/162 (56.17%), Postives = 106/162 (65.43%), Query Frame = 0

Query: 6   SIPTSAFSTVTLLRSLTLSLSPYHHDFRCPNHIILTLFLPAYSVK-VPQLPRIRAFA--S 65
           SI TSAFS+VTLLRS +LSLSPYHH FRCPNHI+ T+F+P YSVK   QLPRI +FA  S
Sbjct: 2   SIHTSAFSSVTLLRSPSLSLSPYHHYFRCPNHIVRTIFIPIYSVKGQQQLPRIPSFASSS 61

Query: 66  VLHRWV----------------------------CNIWERQPLEVKELDESSEQWRISRL 125
            + + V                                E   LEVKELDE  +QWR S+L
Sbjct: 62  SVEQLVYDRDSLFESEEHLSSPYSNGADGFASADLKHLEMPALEVKELDELPDQWRRSKL 121

Query: 126 AWLCKKFPARKPRIVIRLLNAQKKWMGRDDATYLTVHCLRIR 137
           AWLCK+ PA+KP  +IRLLNAQ+KWM +DDATYLTVHCLRIR
Sbjct: 122 AWLCKELPAQKPGTLIRLLNAQRKWMRQDDATYLTVHCLRIR 163

BLAST of CSPI03G27340 vs. NCBI nr
Match: KAG6607381.1 (Pentatricopeptide repeat-containing protein, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 142.5 bits (358), Expect = 2.7e-30
Identity = 85/165 (51.52%), Postives = 100/165 (60.61%), Query Frame = 0

Query: 4   STSIPTSAFSTVTLLRSLTLSLSPYHHDFRCPNHIILTLFLPAYSVK-VPQLPRIRAFAS 63
           S SI TSAF+TVTLLRSLTL  S  HH FRC N++I +L +P YS K   QLPRI AFAS
Sbjct: 24  SMSIRTSAFATVTLLRSLTLPFSQCHHHFRCRNYVIRSLCIPTYSAKGRRQLPRIPAFAS 83

Query: 64  VLHRWVCNIWERQ-------------------------------PLEVKELDESSEQWRI 123
                V  +++R                                 LEVKELDE  EQWR 
Sbjct: 84  SSSVEVL-VYDRDSPAESEEPLCSPYSTGAEGFASADLKHLGAPALEVKELDELPEQWRR 143

Query: 124 SRLAWLCKKFPARKPRIVIRLLNAQKKWMGRDDATYLTVHCLRIR 137
           S+LAWLCK+ PA+KP  +IRLLNAQ+KWM +DDA Y+ VHCLRIR
Sbjct: 144 SKLAWLCKELPAQKPGTLIRLLNAQRKWMKQDDAAYVIVHCLRIR 187

BLAST of CSPI03G27340 vs. NCBI nr
Match: XP_022949171.1 (pentatricopeptide repeat-containing protein At2g15820, chloroplastic [Cucurbita moschata])

HSP 1 Score: 142.5 bits (358), Expect = 2.7e-30
Identity = 84/162 (51.85%), Postives = 98/162 (60.49%), Query Frame = 0

Query: 6   SIPTSAFSTVTLLRSLTLSLSPYHHDFRCPNHIILTLFLPAYSVK-VPQLPRIRAFAS-- 65
           SI TSAF+TVTLLRSLTL  S  HH FRC N++I +L +P YS K   QLPRI AFAS  
Sbjct: 2   SIRTSAFATVTLLRSLTLPFSQCHHHFRCRNYVIRSLCIPTYSAKGRRQLPRIPAFASSS 61

Query: 66  -------------VLHRWVCNIWE---------------RQPLEVKELDESSEQWRISRL 125
                             +C+ +                   LEVKELDE  EQWR S+L
Sbjct: 62  SVEALVYDRDSPAESEEPLCSPYSTGAEGFASADLKHLGAPALEVKELDELPEQWRRSKL 121

Query: 126 AWLCKKFPARKPRIVIRLLNAQKKWMGRDDATYLTVHCLRIR 137
           AWLCK+ PA+KP  +IRLLNAQ+KWM +DDA YL VHCLRIR
Sbjct: 122 AWLCKELPAQKPGTLIRLLNAQRKWMKQDDAAYLIVHCLRIR 163

BLAST of CSPI03G27340 vs. TAIR 10
Match: AT2G15820.1 (endonucleases )

HSP 1 Score: 87.4 bits (215), Expect = 9.5e-18
Identity = 36/59 (61.02%), Postives = 49/59 (83.05%), Query Frame = 0

Query: 78  EVKELDESSEQWRISRLAWLCKKFPARKPRIVIRLLNAQKKWMGRDDATYLTVHCLRIR 137
           EV+EL+E  E+WR S+LAWLCK+ P  K   ++RLLNAQKKW+ ++DATY++VHC+RIR
Sbjct: 158 EVRELEELPEEWRRSKLAWLCKEVPTHKAVTLVRLLNAQKKWVRQEDATYISVHCMRIR 216

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9XIL51.3e-1661.02Pentatricopeptide repeat-containing protein At2g15820, chloroplastic OS=Arabidop... [more]
Q6ZHJ55.1e-1663.33Pentatricopeptide repeat-containing protein OTP51, chloroplastic OS=Oryza sativa... [more]
Match NameE-valueIdentityDescription
A0A1S3CPK04.3e-4261.05pentatricopeptide repeat-containing protein At2g15820, chloroplastic OS=Cucumis ... [more]
A0A0A0LBL01.3e-4160.69LAGLIDADG_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G625100... [more]
A0A6J1GB981.3e-3051.85pentatricopeptide repeat-containing protein At2g15820, chloroplastic OS=Cucurbit... [more]
A0A6J1KB641.4e-2951.23pentatricopeptide repeat-containing protein At2g15820, chloroplastic OS=Cucurbit... [more]
A0A6J1DXY92.1e-2546.20pentatricopeptide repeat-containing protein At2g15820, chloroplastic-like OS=Mom... [more]
Match NameE-valueIdentityDescription
XP_008465080.18.9e-4261.05PREDICTED: pentatricopeptide repeat-containing protein At2g15820, chloroplastic ... [more]
XP_004152074.22.6e-4160.69pentatricopeptide repeat-containing protein At2g15820, chloroplastic [Cucumis sa... [more]
XP_038887990.11.2e-3556.17pentatricopeptide repeat-containing protein At2g15820, chloroplastic [Benincasa ... [more]
KAG6607381.12.7e-3051.52Pentatricopeptide repeat-containing protein, chloroplastic, partial [Cucurbita a... [more]
XP_022949171.12.7e-3051.85pentatricopeptide repeat-containing protein At2g15820, chloroplastic [Cucurbita ... [more]
Match NameE-valueIdentityDescription
AT2G15820.19.5e-1861.02endonucleases [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR47539PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN OTP51, CHLOROPLASTICcoord: 74..136

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI03G27340.1CSPI03G27340.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006397 mRNA processing
biological_process GO:0000377 RNA splicing, via transesterification reactions with bulged adenosine as nucleophile