CSPI03G27050 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI03G27050
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Descriptionribokinase isoform X1
LocationChr3: 24465392 .. 24475731 (+)
RNA-Seq ExpressionCSPI03G27050
SyntenyCSPI03G27050
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACGCCATTGCCACCTCTCCTTTCAAGCAATAAGCTTCCTCTTCATTCTTCTCCAACCTTTATTCTCTTCTCTAAATCAATCAGGTCTGTTTTACCTTTCTCTTGCAATCTCTAACTTCACATTCACCTTTTTTCCGATCAAATCCAAACTAATTCTATACGATTTCAGCCGTTTCTATTTTCTGATTTCAGGCCGACAATGTCATCGTCAACCACCTCCTCGCGTTCCATTTCATTTCCTGAAAATCGAGTGGTTGTGAGTCTCTCTCTCTCTCTCTCTCCTTGCAATGCTTGACTGCTTTGGCTCTGTTATTAACATAAACCGTTTCATTTGTATCGTTTTTGAATCATTGGAAGTGCTTGAATGGTTACGTGTGGTTTTGCAGCTTGGTGTTGGTTCTGTTGCTGTTGATTTTTTGGCGGCAGTGGCTTCTTATCCAAATCCTGATGACAAAATTAGAACCACGAGCTTAAAGGTTTGCGTTCTTCTTTGTTAGCGATATGGTCGAGATATCTGTTCCTTTTTCTGTGGTTTCCTGACCATTTTTTGGACTTGTTTTGTGTATGATATGTAGATTTTGTTCATGATGAAAGATTCAATATTGACTGAATCGATTCGGATTTATTGAAGTTTTTGATATTGCTGGAAATTGTAATGTTTTCAGTTCAAGTCTTTTTAATTCCTCAAGTCCTGATAGATGTCAAGTCACCGTTGGAGTTTTAAGATCGCGTTTAGCAGAAAAATTTGTTTACAACTACTCAGCAACCTTTTGACTGTTGTTAATTCATATACTAATGTAATGGTCAATATCTGGACTGGAAGTGAATATGTTTGGAGTAGAACTTAGAATATAACAGTTGATAGTTTTCATCATCTATATGGCTTAGTTAGAGACTCGCTCATTGATATAAGTTCAGTTCAGATTAAAGGAAATGGAGATAGGGAGCAAAGGAGCATTACATTGCTGCAGCACAACACAAAGTCATATATTTGAGAAGGAAGCGACATGGTGCTACACTAGAGCAACATGGTGCAAGAACATGAAAATTTACATTTTCTCCTTTATTTGTTCAAAGGGAATGATGGATAGGCTATTGAGGGTGTTTTCGAGTTCAAAGTTTACAGAGAAAAATAGAGGAACACTCGTGAGATAGAAACCCTTGAAATCTTCATCAATTTTTGTAGAATCTGTAGTTGGAGAAGGCTGAAAATACCCCAAAGTTTTGAGTGAACTAGTATGAACTCGAGTTGTTGCTTCCTTATTCCTATCTTTTCTCCATTAATAATTTACTTTATTTTTTTCTGCTTTTTTATCCAACGGAAGGAAAGTTTTAAGCAAGATTTAAAAATGCCCCTAAATTTCCTAAATGATATTGTGAAATGTATTGGTTCAAGTTCAATTATCTCCTCCTTGTAATAAGTTTAATTTATGATTGAATTTTTAAATCCTGGTGTATTCTGAATGAAATATGTTCTTACAACAAAAAGTTGTCGTTTCTATTTACTGTACCTAAGATGACTTCTTCTTAGGTACAAGGTGGAGGAAATGTAGGAAATGCTTTAACTTCTGCAGCTCGGTTGGGTTTAACACCAAGGATAATTTCCAAGGTTTCTCTTTATCCATGACATCTTTCCTTTAACCAACCCAACTTTTAAGTACTAAATTGAACTGGTGTATACATATGTATTATTTACTAACATATTCATGCACGATAAAATTGTAGGTTGCAGATGATAGTCAAGGAAGGAGCATAATTGAGGAGCTAGAAGCCGATGGCGTAGATACATCTTTTCTTGTGGTAAGCTCTTCATAACAATTTTAAGTATTTGTGACTTGCGTATCATAATTGGCTTTGCATTATCTTGACAGTACTGCCTTTGTACTGTATTTCGTACTCAGTATATGCTTTGTGTACGTTTTTAATTTTTTTTAAAACAATTATAATTATAACAAGAAACAAGACTTTCATTGGAGAAACGAAAAGTGATTAATGCTTTAAAAATATAAAAACTTCAGAAGATAAGGGAGTGAATAAGAACAAAAATAAAAGAAAAAAAAAACTCCACATGAAAAATAAAGGCGTCCCAAGAAAAAATAATATTTAACAAAGAAAAAATAGCAAAAGACTTGGTGAGAGAGCACTGGGAGGAGGCTTTCAATTTAGTCGACTCAAATGGATCTAACTAAGGAAGGTGTTTGCTTTCATAATTTTCAAATCTATTTCCATCCACATTTTTGAAAGGAGAGGTTTATTCACATTGATCCATAGATTTGAGAGGAGAAGCGGAGGAACATTTTCTTTTAAATTAGAAGATAACACCAATGATAAACTGAATAAGGACAAAAGCTGTGAACAAGTACAACCAAAGAAAATATGATAGAGATAATCTCATTTTCATGGCAAAGGGGACAAACGAGAGTATCCAAATTGGGAAAGTCTTGCTTATTATTATCATCTTTTGTTGGTGATGCAAGTGTAACGGTGATAGATGTTTGTCTACAGACTATTAAGTTTAATGCTAGTGTATTGTTTACTAGTTGAAGTGACTTATCTAGAAATTGGAAAAACACATTTTGTGGTGTTGAGTTCTAATTCCAATATATGGTTGTACAGAAAACTGAAGCAAGAGTGTAATGGACTTTTGAGGCTCTGAGGGAAAAAGGAAACATCAAAGGACATAAACTTAAATTTCTTGTGGTGAAATCTGCATTGATTGTATTTACTAATTTAAGTGAAATTTACTTATCATTCAATTGCATCACTGTCTGCCATGGAATATTGTGGTATAGCTTTGATGTTCCAAACTGATATGTTGAAGTTTGACATTTTGAATGACATTGAGAACTTTCCACTTTTCAAACCTCTGTTTGGTTTGTATCTTAATTAACAGTTGTTGAAATTATAAAAATGGTCACCTACACTTGTAAGATATTTAATTATCGTTTATTTCTTCTTTTCAGTGAAATATATATTTTGCACCTATTCTTTTGTCAGAAGTTTAAAGTCACACACTCATATTTATTGTGCTAAAAGTTTCTGATGTGTTGCCATATATATTTCGTCTTTTATCTACTATTTACTCTGAAAGAATATTTGCTTTTTTTTCTTTTTTTTTTTATAATGGGTCCTTAGTGACTTCCTATGAGTTACCACATTTGTGATTTTTTATGTTTTCTCAGGTTTCTGAAGGGGGCATTTCACCATTTACCTATATTATTGTGGATAATAAAACGTAAGTATTTGGATTTCCAGGCTCCTCTACTCTGTAATTGAGTGTGAGGGCTGTCTTGTTTTTGGCTTGTTTTGGTTTTGGTCTTACCCGTGTTTCCTTTCATCTATCTCGATGAAAGTTTAGTTTCTTATAGAAGTAAAAAGGAATGAGTGTCTTTTCATGTTAGGCATGTGTGAGACCCCCAATCATACATACGTATGTTAGGAAGGGTAAGAAGGGAAACTCACCAACATGTAAGGAGAGAGAATGAGTGGGAAATAAAAGAATGTGCAGGTGGGTCCCACTGGGTAGGAATTTGAGGGTGAGTATAAATAGAATGATTGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN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TGTTTCCATGCTTGTGCGACTTCCAAAACTCAGATTTGTGATTGTCACATTGGGTGAAAATGGATGCATAATGCTCGAAAGAAGTACTGATGGTGAGTTTTTATAATCAACTTCTACTATGCCGTTGGCTATGATACTGTTCTCCCTTTAGGGTGGATGTTGTTTAGGTACAAATTTTTTTGAATTTAATTTATATATTTAAAATTTACAGGTATTTTCTCTATTTCTGGCAGAAAAATATGATCAGATGGAAGAGTTTGAAGTAGACAGCTTACTTGAAGTGGTCAAGAGCAGAAGAAATGAGAATATAAATGTTCCAACTTCTGTTTCATCGGTAATT

mRNA sequence

ATGACGCCATTGCCACCTCTCCTTTCAAGCAATAAGCTTCCTCTTCATTCTTCTCCAACCTTTATTCTCTTCTCTAAATCAATCAGGCCGACAATGTCATCGTCAACCACCTCCTCGCGTTCCATTTCATTTCCTGAAAATCGAGTGGTTCTTGGTGTTGGTTCTGTTGCTGTTGATTTTTTGGCGGCAGTGGCTTCTTATCCAAATCCTGATGACAAAATTAGAACCACGAGCTTAAAGGTACAAGGTGGAGGAAATGTAGGAAATGCTTTAACTTCTGCAGCTCGGTTGGGTTTAACACCAAGGATAATTTCCAAGGTTGCAGATGATAGTCAAGGAAGGAGCATAATTGAGGAGCTAGAAGCCGATGGCGTAGATACATCTTTTCTTGTGGTTTCTGAAGGGGGCATTTCACCATTTACCTATATTATTGTGGATAATAAAACGCACACTCGTACATGCATCCACACCCCAGGATCTCCTCCCATGGTGCCAGATGACCTTTCGCGATCAAGCTTGTTGTCTGCTTTAGATGGGGCTAAAATTGTATATTCTGACATAAGATTGCACGAAACTGCTTTGCTTGTTGCACAAGAGGCAGCTCGGCAGAATATACCTATGTTGATTGATGCAGAAAGGAAAAGGGAAGGTCTGGATGATCTCCTGGCATTTGCCAGTTATGTTGTCTGTTCAACAAGTTTTCCACAGGCAAAAACATTATTTAATGGGCAGGAATGGACTGAGGCACCATCGATTCCATCTGCACTTGTTTCCATGCTTGTGCGACTTCCAAAACTCAGATTTGTGATTGTCACATTGGGTGAAAATGGATGCATAATGCTCGAAAGAAGTACTGATGAAAAATATGATCAGATGGAAGAGTTTGAAGTAGACAGCTTACTTGAAGTGGTCAAGAGCAGAAGAAATGAGAATATAAATGTTCCAACTTCTGTTTCATCGGTAATT

Coding sequence (CDS)

ATGACGCCATTGCCACCTCTCCTTTCAAGCAATAAGCTTCCTCTTCATTCTTCTCCAACCTTTATTCTCTTCTCTAAATCAATCAGGCCGACAATGTCATCGTCAACCACCTCCTCGCGTTCCATTTCATTTCCTGAAAATCGAGTGGTTCTTGGTGTTGGTTCTGTTGCTGTTGATTTTTTGGCGGCAGTGGCTTCTTATCCAAATCCTGATGACAAAATTAGAACCACGAGCTTAAAGGTACAAGGTGGAGGAAATGTAGGAAATGCTTTAACTTCTGCAGCTCGGTTGGGTTTAACACCAAGGATAATTTCCAAGGTTGCAGATGATAGTCAAGGAAGGAGCATAATTGAGGAGCTAGAAGCCGATGGCGTAGATACATCTTTTCTTGTGGTTTCTGAAGGGGGCATTTCACCATTTACCTATATTATTGTGGATAATAAAACGCACACTCGTACATGCATCCACACCCCAGGATCTCCTCCCATGGTGCCAGATGACCTTTCGCGATCAAGCTTGTTGTCTGCTTTAGATGGGGCTAAAATTGTATATTCTGACATAAGATTGCACGAAACTGCTTTGCTTGTTGCACAAGAGGCAGCTCGGCAGAATATACCTATGTTGATTGATGCAGAAAGGAAAAGGGAAGGTCTGGATGATCTCCTGGCATTTGCCAGTTATGTTGTCTGTTCAACAAGTTTTCCACAGGCAAAAACATTATTTAATGGGCAGGAATGGACTGAGGCACCATCGATTCCATCTGCACTTGTTTCCATGCTTGTGCGACTTCCAAAACTCAGATTTGTGATTGTCACATTGGGTGAAAATGGATGCATAATGCTCGAAAGAAGTACTGATGAAAAATATGATCAGATGGAAGAGTTTGAAGTAGACAGCTTACTTGAAGTGGTCAAGAGCAGAAGAAATGAGAATATAAATGTTCCAACTTCTGTTTCATCGGTAATT

Protein sequence

MTPLPPLLSSNKLPLHSSPTFILFSKSIRPTMSSSTTSSRSISFPENRVVLGVGSVAVDFLAAVASYPNPDDKIRTTSLKVQGGGNVGNALTSAARLGLTPRIISKVADDSQGRSIIEELEADGVDTSFLVVSEGGISPFTYIIVDNKTHTRTCIHTPGSPPMVPDDLSRSSLLSALDGAKIVYSDIRLHETALLVAQEAARQNIPMLIDAERKREGLDDLLAFASYVVCSTSFPQAKTLFNGQEWTEAPSIPSALVSMLVRLPKLRFVIVTLGENGCIMLERSTDEKYDQMEEFEVDSLLEVVKSRRNENINVPTSVSSVI
Homology
BLAST of CSPI03G27050 vs. ExPASy Swiss-Prot
Match: P50053 (Ketohexokinase OS=Homo sapiens OX=9606 GN=KHK PE=1 SV=2)

HSP 1 Score: 63.2 bits (152), Expect = 6.4e-09
Identity = 47/191 (24.61%), Postives = 78/191 (40.84%), Query Frame = 0

Query: 46  ENRVVLGVGSVAVDFLAAVASYPNPDDKIRTTSLKVQGGGNVGNALTSAARLGLTPRIIS 105
           E + +L VG V +D ++ V  YP  D +IR  S + Q GGN  N+ T  + LG     + 
Sbjct: 2   EEKQILCVGLVVLDVISLVDKYPKEDSEIRCLSQRWQRGGNASNSCTVLSLLGAPCAFMG 61

Query: 106 KVADDSQGRSIIEELEADGVDTSFLVVSEGGISPFTYIIVDNKTHTRTCI-HTPGSPPMV 165
            +A       ++ +    GVD S +     G +P +  I++N    RT + H    P + 
Sbjct: 62  SMAPGHVADFLVADFRRRGVDVSQVAWQSKGDTPSSCCIINNSNGNRTIVLHDTSLPDVS 121

Query: 166 PDDLSRSSLLS----ALDGAKIVYSDIRLHETALLVAQEAARQNIPMLIDAERKREGLDD 225
             D  +  L       ++G         L        ++   Q I + ++ E+ RE L  
Sbjct: 122 ATDFEKVDLTQFKWIHIEGRNASEQVKMLQRIDAHNTRQPPEQKIRVSVEVEKPREELFQ 181

Query: 226 LLAFASYVVCS 232
           L  +   V  S
Sbjct: 182 LFGYGDVVFVS 192

BLAST of CSPI03G27050 vs. ExPASy Swiss-Prot
Match: E9AD19 (Ribokinase OS=Leishmania major OX=5664 GN=LMJF_27_0420 PE=1 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 9.2e-08
Identity = 55/247 (22.27%), Postives = 105/247 (42.51%), Query Frame = 0

Query: 50  VLGVGSVAVDFLAAVASYPNPDDKIRTTSLKVQGGGNVGNALTSAARLGLTPRIISKVAD 109
           +L VGS  +D++  V   P   + + + S     GG   N   +A RLG    ++S V  
Sbjct: 18  ILVVGSCFLDYVGYVDHMPQVGETMHSESFHKGFGGKGANQAVAAGRLGAKVAMVSMVGT 77

Query: 110 DSQGRSIIEELEADGVDTSFLVVSEGGISPFTYIIVDNKTHTRTCIHTPGSPPMVPDDLS 169
           D  G   I+ELE +GV T++++ +    +    I+VD K+     +  P +      +L 
Sbjct: 78  DGDGSDYIKELERNGVHTAYMLRTGKSSTGLAMILVDTKSSNNEIVICPNATNYFTPELL 137

Query: 170 RSSLLSALDGAKIVYSDIRL--------HETALLVAQEAARQNIPMLIDA--ERKREGLD 229
           R+      +  KI+++ ++           T L   +EA  + +  + ++    K   ++
Sbjct: 138 RA---QTSNYEKILHTGLKYLICQNEIPLPTTLDTIKEAHSRGVYTVFNSAPAPKPAEVE 197

Query: 230 DLLAFASYVVCSTSFPQAKTLFNGQEWTEAPSIPSALVSMLVRLPKLRFVIVTLGENGCI 287
            +  F  YV           L  G + T+  S   A+ ++  +   +R V++TLG  G  
Sbjct: 198 QIKPFLPYVSLFCPNEVEAALITGMKVTDTESAFRAIKAL--QQLGVRDVVITLGAAGFA 257

BLAST of CSPI03G27050 vs. ExPASy Swiss-Prot
Match: Q02974 (Ketohexokinase OS=Rattus norvegicus OX=10116 GN=Khk PE=1 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 1.6e-07
Identity = 47/191 (24.61%), Postives = 77/191 (40.31%), Query Frame = 0

Query: 46  ENRVVLGVGSVAVDFLAAVASYPNPDDKIRTTSLKVQGGGNVGNALTSAARLGLTPRIIS 105
           E + +L VG V +D +  V  YP  D   R  S + Q GGN  N+ T  + LG     + 
Sbjct: 2   EEKQILCVGLVVLDIINVVDKYPEEDTDRRCLSQRWQRGGNASNSCTVLSLLGARCAFMG 61

Query: 106 KVADDSQGRSIIEELEADGVDTSFLVVSEGGISPFTYIIVDNKTHTRTCI-HTPGSPPMV 165
            +A       ++ +    GVD S +     G +P +  IV+N   +RT I +    P + 
Sbjct: 62  SLAHGHVADFLVADFRRRGVDVSQVAWQSQGDTPCSCCIVNNSNGSRTIILYDTNLPDVS 121

Query: 166 PDDLSRSSLLS----ALDGAKIVYSDIRLHETALLVAQEAARQNIPMLIDAERKREGLDD 225
             D  +  L       ++G         L       A +  +Q + + ++ E+ RE L  
Sbjct: 122 AKDFEKVDLTRFKWIHIEGRNASEQVKMLQRIEQYNATQPLQQKVRVSVEIEKPREELFQ 181

Query: 226 LLAFASYVVCS 232
           L  +   V  S
Sbjct: 182 LFGYGEVVFVS 192

BLAST of CSPI03G27050 vs. ExPASy Swiss-Prot
Match: P97328 (Ketohexokinase OS=Mus musculus OX=10090 GN=Khk PE=1 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 3.5e-07
Identity = 47/191 (24.61%), Postives = 78/191 (40.84%), Query Frame = 0

Query: 46  ENRVVLGVGSVAVDFLAAVASYPNPDDKIRTTSLKVQGGGNVGNALTSAARLGLTPRIIS 105
           E + +L VG V +D +  V  YP  D   R  S + Q GGN  N+ T  + LG     + 
Sbjct: 2   EEKQILCVGLVVLDIINVVDKYPEEDTDRRCLSQRWQRGGNASNSCTVLSLLGARCAFMG 61

Query: 106 KVADDSQGRSIIEELEADGVDTSFLVVSEGGISPFTYIIVDNKTHTRTCI-HTPGSPPMV 165
            +A       ++ +    GVD S +     G +P +  IV+N   +RT I +    P + 
Sbjct: 62  SLAPGHVADFLVADFRQRGVDVSQVTWQSQGDTPCSCCIVNNSNGSRTIILYDTNLPDVS 121

Query: 166 PDDLSRSSLLS----ALDGAKIVYSDIRLHETALLVAQEAARQNIPMLIDAERKREGLDD 225
             D  +  L       ++G         L       A++   Q + + ++ E+ RE L  
Sbjct: 122 AKDFEKVDLTRFKWIHIEGRNASEQVKMLQRIEEHNAKQPLPQKVRVSVEIEKPREELFQ 181

Query: 226 LLAFASYVVCS 232
           L ++   V  S
Sbjct: 182 LFSYGEVVFVS 192

BLAST of CSPI03G27050 vs. ExPASy Swiss-Prot
Match: Q5RD71 (Ketohexokinase OS=Pongo abelii OX=9601 GN=KHK PE=3 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 3.5e-07
Identity = 66/279 (23.66%), Postives = 112/279 (40.14%), Query Frame = 0

Query: 46  ENRVVLGVGSVAVDFLAAVASYPNPDDKIRTTSLKVQGGGNVGNALTSAARLGLTPRIIS 105
           E + +L VG V +D ++ V  YP  D +IR  S + Q GGN  N+ T  + LG     + 
Sbjct: 2   EEKQILCVGLVVLDVISLVDKYPKEDSEIRCLSQRWQRGGNASNSCTVLSLLGAPCAFMG 61

Query: 106 KVADDSQGRSIIEELEADGVDTSFLVVSEGGISPFTYIIVDNKTHTRTCI-HTPGSPPMV 165
            +A       ++ +    GVD S +     G +P +  I++N    RT + H    P + 
Sbjct: 62  SMA-PGHVAFLVADFRRRGVDVSQVAWQSKGDTPSSCCIINNSNGNRTIVLHDTSLPDVS 121

Query: 166 PDDLSRSSLLS----ALDGAKIVYSDIRLHETALLVAQEAARQNIPMLIDAERKREGLDD 225
             D  +  L       ++G         L        ++   Q I + ++ E+ +E L  
Sbjct: 122 ATDFEKVDLTQFKWIHIEGRNASEQVKMLQRIDTHNTRQPPEQKIRVSVEVEKPQEELFQ 181

Query: 226 LLAFASYVVCSTSFPQAKTLFNGQEWTEAPSIPSALVSMLVRLPKLRFVIVTLGENGCIM 285
           L  +   V  S     AK L  G +  EA     AL  +  R+ K   ++    E G   
Sbjct: 182 LFGYGDVVFVSKDV--AKHL--GFQSAEA-----ALRGLYGRVRKGAVLVCAWAEEGADA 241

Query: 286 LERSTDEKYDQMEEFEVDSLLEVVKSRRNENINVPTSVS 320
           L    D+K    + F    +++ + +    N +V  S+S
Sbjct: 242 L--GPDDKLFHSDAFPPPRVVDTLGAGDTFNASVIFSLS 268

BLAST of CSPI03G27050 vs. ExPASy TrEMBL
Match: A0A1S3BH87 (ribokinase isoform X1 OS=Cucumis melo OX=3656 GN=LOC103489824 PE=3 SV=1)

HSP 1 Score: 549.7 bits (1415), Expect = 8.3e-153
Identity = 292/322 (90.68%), Postives = 304/322 (94.41%), Query Frame = 0

Query: 1   MTPLPPLLSSNKLPLHSSPTFILFSKSIRPTMSSSTTSSRSISFPENRVVLGVGSVAVDF 60
           MTPLPPLLSSNKL  HSSPT +L SKSIRPTMSSSTTSS SISFPENRVVLGVG+V+VDF
Sbjct: 1   MTPLPPLLSSNKLLPHSSPTSLL-SKSIRPTMSSSTTSSFSISFPENRVVLGVGTVSVDF 60

Query: 61  LAAVASYPNPDDKIRTTSLKVQGGGNVGNALTSAARLGLTPRIISKVADDSQGRSIIEEL 120
           LAAVASYPNPDDKIRTTSLKVQGGGNVGNALTSAARLGLTPRIISKVADDSQGRSIIEEL
Sbjct: 61  LAAVASYPNPDDKIRTTSLKVQGGGNVGNALTSAARLGLTPRIISKVADDSQGRSIIEEL 120

Query: 121 EADGVDTSFLVVSEGGISPFTYIIVDNKTHTRTCIHTPGSPPMVPDDLSRSSLLSALDGA 180
           EADGVDTSFLVV+EGGISPFTYIIVDNKTHTRTCIHTPGSPPMVPDDLSRSSLLSALDGA
Sbjct: 121 EADGVDTSFLVVAEGGISPFTYIIVDNKTHTRTCIHTPGSPPMVPDDLSRSSLLSALDGA 180

Query: 181 KIVYSDIRLHETALLVAQEAARQNIPMLIDAERKREGLDDLLAFASYVVCSTSFPQAKTL 240
           +IVYSD+RLHETALLVAQEAARQNIPMLIDAERKREGLDDLL FASYVVCST FP     
Sbjct: 181 RIVYSDVRLHETALLVAQEAARQNIPMLIDAERKREGLDDLLTFASYVVCSTRFP----- 240

Query: 241 FNGQEWTEAPSIPSALVSMLVRLPKLRFVIVTLGENGCIMLERSTDEKYDQMEEFEVDSL 300
              QEWT+ P+IPSALVSML+RLPKLRFVIVTLGENGCIMLERSTDE YDQMEEFEV++L
Sbjct: 241 ---QEWTDTPTIPSALVSMLLRLPKLRFVIVTLGENGCIMLERSTDETYDQMEEFEVNNL 300

Query: 301 LEVVKSRRNENINVPTSVSSVI 323
           LEVVKSRRNENINVPTSVSS +
Sbjct: 301 LEVVKSRRNENINVPTSVSSPV 313

BLAST of CSPI03G27050 vs. ExPASy TrEMBL
Match: A0A1S3BH76 (ribokinase isoform X2 OS=Cucumis melo OX=3656 GN=LOC103489824 PE=3 SV=1)

HSP 1 Score: 509.6 bits (1311), Expect = 9.5e-141
Identity = 266/291 (91.41%), Postives = 277/291 (95.19%), Query Frame = 0

Query: 32  MSSSTTSSRSISFPENRVVLGVGSVAVDFLAAVASYPNPDDKIRTTSLKVQGGGNVGNAL 91
           MSSSTTSS SISFPENRVVLGVG+V+VDFLAAVASYPNPDDKIRTTSLKVQGGGNVGNAL
Sbjct: 1   MSSSTTSSFSISFPENRVVLGVGTVSVDFLAAVASYPNPDDKIRTTSLKVQGGGNVGNAL 60

Query: 92  TSAARLGLTPRIISKVADDSQGRSIIEELEADGVDTSFLVVSEGGISPFTYIIVDNKTHT 151
           TSAARLGLTPRIISKVADDSQGRSIIEELEADGVDTSFLVV+EGGISPFTYIIVDNKTHT
Sbjct: 61  TSAARLGLTPRIISKVADDSQGRSIIEELEADGVDTSFLVVAEGGISPFTYIIVDNKTHT 120

Query: 152 RTCIHTPGSPPMVPDDLSRSSLLSALDGAKIVYSDIRLHETALLVAQEAARQNIPMLIDA 211
           RTCIHTPGSPPMVPDDLSRSSLLSALDGA+IVYSD+RLHETALLVAQEAARQNIPMLIDA
Sbjct: 121 RTCIHTPGSPPMVPDDLSRSSLLSALDGARIVYSDVRLHETALLVAQEAARQNIPMLIDA 180

Query: 212 ERKREGLDDLLAFASYVVCSTSFPQAKTLFNGQEWTEAPSIPSALVSMLVRLPKLRFVIV 271
           ERKREGLDDLL FASYVVCST FP        QEWT+ P+IPSALVSML+RLPKLRFVIV
Sbjct: 181 ERKREGLDDLLTFASYVVCSTRFP--------QEWTDTPTIPSALVSMLLRLPKLRFVIV 240

Query: 272 TLGENGCIMLERSTDEKYDQMEEFEVDSLLEVVKSRRNENINVPTSVSSVI 323
           TLGENGCIMLERSTDE YDQMEEFEV++LLEVVKSRRNENINVPTSVSS +
Sbjct: 241 TLGENGCIMLERSTDETYDQMEEFEVNNLLEVVKSRRNENINVPTSVSSPV 283

BLAST of CSPI03G27050 vs. ExPASy TrEMBL
Match: A0A6J1HH81 (ribokinase-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111464044 PE=3 SV=1)

HSP 1 Score: 498.0 bits (1281), Expect = 2.9e-137
Identity = 262/323 (81.11%), Postives = 288/323 (89.16%), Query Frame = 0

Query: 3   PLPPLLSSNKL---PLHSSPTFILFSKSIRPTMSSSTTSSRSISFPENRVVLGVGSVAVD 62
           PLP  +SS  L   PL  +  F+      RP +SSST+SS SIS P+NRVVLGVGS  VD
Sbjct: 2   PLPAFISSTTLVHSPLRINQPFLCAYSGFRPRISSSTSSSVSISLPDNRVVLGVGSATVD 61

Query: 63  FLAAVASYPNPDDKIRTTSLKVQGGGNVGNALTSAARLGLTPRIISKVADDSQGRSIIEE 122
           FLAAVASYPNPDDKIRTTSLKVQGGGNVGNALTSAARLGLTPRIISKVADDSQGRSI+EE
Sbjct: 62  FLAAVASYPNPDDKIRTTSLKVQGGGNVGNALTSAARLGLTPRIISKVADDSQGRSIVEE 121

Query: 123 LEADGVDTSFLVVSEGGISPFTYIIVDNKTHTRTCIHTPGSPPMVPDDLSRSSLLSALDG 182
           LEADG+DTSFLVV+EGG+SPFTYIIVDNKTHTRTCIHTPGSPPMVPDDLSRSSLLSALDG
Sbjct: 122 LEADGIDTSFLVVAEGGVSPFTYIIVDNKTHTRTCIHTPGSPPMVPDDLSRSSLLSALDG 181

Query: 183 AKIVYSDIRLHETALLVAQEAARQNIPMLIDAERKREGLDDLLAFASYVVCSTSFPQAKT 242
           A+IVY D+RLHETALLVA+EAA+QNIP+LIDAERKREGLD+LLAFASYVVCSTSFP    
Sbjct: 182 ARIVYFDVRLHETALLVAEEAAQQNIPILIDAERKREGLDELLAFASYVVCSTSFP---- 241

Query: 243 LFNGQEWTEAPSIPSALVSMLVRLPKLRFVIVTLGENGCIMLERSTDEKYDQMEEFEVDS 302
               QEWTEAPSIPSALVSML+RLPKL+FVIVTLGE GCIMLERST+EK +Q+EEFEVD+
Sbjct: 242 ----QEWTEAPSIPSALVSMLLRLPKLKFVIVTLGEEGCIMLERSTNEKPEQIEEFEVDN 301

Query: 303 LLEVVKSRRNENINVPTSVSSVI 323
           LLEV+K R++ENINVPTSVSS +
Sbjct: 302 LLEVIKGRKDENINVPTSVSSPV 316

BLAST of CSPI03G27050 vs. ExPASy TrEMBL
Match: A0A6J1HTZ4 (ribokinase-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111466847 PE=3 SV=1)

HSP 1 Score: 494.6 bits (1272), Expect = 3.2e-136
Identity = 262/323 (81.11%), Postives = 284/323 (87.93%), Query Frame = 0

Query: 3   PLPPLLSSNKL---PLHSSPTFILFSKSIRPTMSSSTTSSRSISFPENRVVLGVGSVAVD 62
           PLP  +SS  L   PL  +  F+       P MSSST+SS SIS PENRVVLGVGS  VD
Sbjct: 2   PLPAFISSTTLVHSPLQINQRFLCAYSGFWPRMSSSTSSSVSISLPENRVVLGVGSATVD 61

Query: 63  FLAAVASYPNPDDKIRTTSLKVQGGGNVGNALTSAARLGLTPRIISKVADDSQGRSIIEE 122
           FLAAVASYPNPDDKIRTTSLKVQGGGNVGNALTSAARLGLTPRIISKVADDSQGRSI+EE
Sbjct: 62  FLAAVASYPNPDDKIRTTSLKVQGGGNVGNALTSAARLGLTPRIISKVADDSQGRSIVEE 121

Query: 123 LEADGVDTSFLVVSEGGISPFTYIIVDNKTHTRTCIHTPGSPPMVPDDLSRSSLLSALDG 182
           LEADG+DTSFLVV+EGG+SPFTYIIVDNKTHTRTCIHTPGSPPMVPDDLSRSSLLSALDG
Sbjct: 122 LEADGIDTSFLVVAEGGVSPFTYIIVDNKTHTRTCIHTPGSPPMVPDDLSRSSLLSALDG 181

Query: 183 AKIVYSDIRLHETALLVAQEAARQNIPMLIDAERKREGLDDLLAFASYVVCSTSFPQAKT 242
           AKIVY D+RLHETALLVA+EAA+QNIP+LIDAERKREGLD+LLAFASYVVCSTSFP    
Sbjct: 182 AKIVYFDVRLHETALLVAEEAAQQNIPILIDAERKREGLDELLAFASYVVCSTSFP---- 241

Query: 243 LFNGQEWTEAPSIPSALVSMLVRLPKLRFVIVTLGENGCIMLERSTDEKYDQMEEFEVDS 302
               QEWTEAPSIPSALVSML+RLPKL+FVIVTLGE GCIMLERS +E  DQ+EEFEVD+
Sbjct: 242 ----QEWTEAPSIPSALVSMLLRLPKLKFVIVTLGEEGCIMLERSNNETPDQIEEFEVDN 301

Query: 303 LLEVVKSRRNENINVPTSVSSVI 323
           LLE +K R++ENINVPTSVSS +
Sbjct: 302 LLEAIKGRKDENINVPTSVSSPV 316

BLAST of CSPI03G27050 vs. ExPASy TrEMBL
Match: A0A6J1HRJ6 (ribokinase-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111466847 PE=3 SV=1)

HSP 1 Score: 488.4 bits (1256), Expect = 2.3e-134
Identity = 261/323 (80.80%), Postives = 282/323 (87.31%), Query Frame = 0

Query: 3   PLPPLLSSNKL---PLHSSPTFILFSKSIRPTMSSSTTSSRSISFPENRVVLGVGSVAVD 62
           PLP  +SS  L   PL  +  F+       P MSSST+SS SIS PENRVVLGVGS  VD
Sbjct: 2   PLPAFISSTTLVHSPLQINQRFLCAYSGFWPRMSSSTSSSVSISLPENRVVLGVGSATVD 61

Query: 63  FLAAVASYPNPDDKIRTTSLKVQGGGNVGNALTSAARLGLTPRIISKVADDSQGRSIIEE 122
           FLAAVASYPNPDDKIRTTSLKVQGGGNVGNALTSAARLGLTPRIISKVADDSQGRSI+EE
Sbjct: 62  FLAAVASYPNPDDKIRTTSLKVQGGGNVGNALTSAARLGLTPRIISKVADDSQGRSIVEE 121

Query: 123 LEADGVDTSFLVVSEGGISPFTYIIVDNKTHTRTCIHTPGSPPMVPDDLSRSSLLSALDG 182
           LEADG+DTSFLVV+EGG+SPFTYIIVDNKTHTRTCIHTPGSPPMVPDDLSRSSLLSALDG
Sbjct: 122 LEADGIDTSFLVVAEGGVSPFTYIIVDNKTHTRTCIHTPGSPPMVPDDLSRSSLLSALDG 181

Query: 183 AKIVYSDIRLHETALLVAQEAARQNIPMLIDAERKREGLDDLLAFASYVVCSTSFPQAKT 242
           AKIVY D+RLHETALLVA+EAA+QNIP+LIDAERKREGLD+LLAFASYVVCSTSFP    
Sbjct: 182 AKIVYFDVRLHETALLVAEEAAQQNIPILIDAERKREGLDELLAFASYVVCSTSFP---- 241

Query: 243 LFNGQEWTEAPSIPSALVSMLVRLPKLRFVIVTLGENGCIMLERSTDEKYDQMEEFEVDS 302
               QEWTEAPSIPSALVSML+RLPKL+FVIVTLGE GCIMLER T    DQ+EEFEVD+
Sbjct: 242 ----QEWTEAPSIPSALVSMLLRLPKLKFVIVTLGEEGCIMLERKTP---DQIEEFEVDN 301

Query: 303 LLEVVKSRRNENINVPTSVSSVI 323
           LLE +K R++ENINVPTSVSS +
Sbjct: 302 LLEAIKGRKDENINVPTSVSSPV 313

BLAST of CSPI03G27050 vs. NCBI nr
Match: XP_004152009.1 (ribokinase isoform X1 [Cucumis sativus])

HSP 1 Score: 589.0 bits (1517), Expect = 2.5e-164
Identity = 312/322 (96.89%), Postives = 313/322 (97.20%), Query Frame = 0

Query: 1   MTPLPPLLSSNKLPLHSSPTFILFSKSIRPTMSSSTTSSRSISFPENRVVLGVGSVAVDF 60
           MTPLPPLLSSNKLPLHSSPTFILFSKSIRPTMSSSTTSSRSISFPENRVVLGVGSVAVDF
Sbjct: 1   MTPLPPLLSSNKLPLHSSPTFILFSKSIRPTMSSSTTSSRSISFPENRVVLGVGSVAVDF 60

Query: 61  LAAVASYPNPDDKIRTTSLKVQGGGNVGNALTSAARLGLTPRIISKVADDSQGRSIIEEL 120
           LAAVASYPNPDDKIRTTSLKVQGGGNVGNALTSAARLGLTPRIISKVADDSQGRSIIEEL
Sbjct: 61  LAAVASYPNPDDKIRTTSLKVQGGGNVGNALTSAARLGLTPRIISKVADDSQGRSIIEEL 120

Query: 121 EADGVDTSFLVVSEGGISPFTYIIVDNKTHTRTCIHTPGSPPMVPDDLSRSSLLSALDGA 180
           EADGVDTSFLVVSEGGISPFTYIIVDNKTHTRTCIHTPGSPPMVPDDLSRSSLLSALDGA
Sbjct: 121 EADGVDTSFLVVSEGGISPFTYIIVDNKTHTRTCIHTPGSPPMVPDDLSRSSLLSALDGA 180

Query: 181 KIVYSDIRLHETALLVAQEAARQNIPMLIDAERKREGLDDLLAFASYVVCSTSFPQAKTL 240
           KIVYSDIRLHETALLVAQEAARQNIPMLIDAERKREGLDDLLAFASYVVCSTSFP     
Sbjct: 181 KIVYSDIRLHETALLVAQEAARQNIPMLIDAERKREGLDDLLAFASYVVCSTSFP----- 240

Query: 241 FNGQEWTEAPSIPSALVSMLVRLPKLRFVIVTLGENGCIMLERSTDEKYDQMEEFEVDSL 300
              QEWTEAPSIPSALVSMLVRLPKLRFVIVTLGENGCIMLERSTDEKYDQMEEFEVDSL
Sbjct: 241 ---QEWTEAPSIPSALVSMLVRLPKLRFVIVTLGENGCIMLERSTDEKYDQMEEFEVDSL 300

Query: 301 LEVVKSRRNENINVPTSVSSVI 323
           LEVVKSRRNENINVPTSVSS +
Sbjct: 301 LEVVKSRRNENINVPTSVSSPV 314

BLAST of CSPI03G27050 vs. NCBI nr
Match: XP_031738611.1 (ribokinase isoform X2 [Cucumis sativus])

HSP 1 Score: 588.6 bits (1516), Expect = 3.3e-164
Identity = 312/320 (97.50%), Postives = 312/320 (97.50%), Query Frame = 0

Query: 1   MTPLPPLLSSNKLPLHSSPTFILFSKSIRPTMSSSTTSSRSISFPENRVVLGVGSVAVDF 60
           MTPLPPLLSSNKLPLHSSPTFILFSKSIRPTMSSSTTSSRSISFPENRVVLGVGSVAVDF
Sbjct: 1   MTPLPPLLSSNKLPLHSSPTFILFSKSIRPTMSSSTTSSRSISFPENRVVLGVGSVAVDF 60

Query: 61  LAAVASYPNPDDKIRTTSLKVQGGGNVGNALTSAARLGLTPRIISKVADDSQGRSIIEEL 120
           LAAVASYPNPDDKIRTTSLKVQGGGNVGNALTSAARLGLTPRIISKVADDSQGRSIIEEL
Sbjct: 61  LAAVASYPNPDDKIRTTSLKVQGGGNVGNALTSAARLGLTPRIISKVADDSQGRSIIEEL 120

Query: 121 EADGVDTSFLVVSEGGISPFTYIIVDNKTHTRTCIHTPGSPPMVPDDLSRSSLLSALDGA 180
           EADGVDTSFLVVSEGGISPFTYIIVDNKTHTRTCIHTPGSPPMVPDDLSRSSLLSALDGA
Sbjct: 121 EADGVDTSFLVVSEGGISPFTYIIVDNKTHTRTCIHTPGSPPMVPDDLSRSSLLSALDGA 180

Query: 181 KIVYSDIRLHETALLVAQEAARQNIPMLIDAERKREGLDDLLAFASYVVCSTSFPQAKTL 240
           KIVYSDIRLHETALLVAQEAARQNIPMLIDAERKREGLDDLLAFASYVVCSTSFP     
Sbjct: 181 KIVYSDIRLHETALLVAQEAARQNIPMLIDAERKREGLDDLLAFASYVVCSTSFP----- 240

Query: 241 FNGQEWTEAPSIPSALVSMLVRLPKLRFVIVTLGENGCIMLERSTDEKYDQMEEFEVDSL 300
              QEWTEAPSIPSALVSMLVRLPKLRFVIVTLGENGCIMLERSTDEKYDQMEEFEVDSL
Sbjct: 241 ---QEWTEAPSIPSALVSMLVRLPKLRFVIVTLGENGCIMLERSTDEKYDQMEEFEVDSL 300

Query: 301 LEVVKSRRNENINVPTSVSS 321
           LEVVKSRRNENINVPTSVSS
Sbjct: 301 LEVVKSRRNENINVPTSVSS 312

BLAST of CSPI03G27050 vs. NCBI nr
Match: XP_008447353.1 (PREDICTED: ribokinase isoform X1 [Cucumis melo])

HSP 1 Score: 549.7 bits (1415), Expect = 1.7e-152
Identity = 292/322 (90.68%), Postives = 304/322 (94.41%), Query Frame = 0

Query: 1   MTPLPPLLSSNKLPLHSSPTFILFSKSIRPTMSSSTTSSRSISFPENRVVLGVGSVAVDF 60
           MTPLPPLLSSNKL  HSSPT +L SKSIRPTMSSSTTSS SISFPENRVVLGVG+V+VDF
Sbjct: 1   MTPLPPLLSSNKLLPHSSPTSLL-SKSIRPTMSSSTTSSFSISFPENRVVLGVGTVSVDF 60

Query: 61  LAAVASYPNPDDKIRTTSLKVQGGGNVGNALTSAARLGLTPRIISKVADDSQGRSIIEEL 120
           LAAVASYPNPDDKIRTTSLKVQGGGNVGNALTSAARLGLTPRIISKVADDSQGRSIIEEL
Sbjct: 61  LAAVASYPNPDDKIRTTSLKVQGGGNVGNALTSAARLGLTPRIISKVADDSQGRSIIEEL 120

Query: 121 EADGVDTSFLVVSEGGISPFTYIIVDNKTHTRTCIHTPGSPPMVPDDLSRSSLLSALDGA 180
           EADGVDTSFLVV+EGGISPFTYIIVDNKTHTRTCIHTPGSPPMVPDDLSRSSLLSALDGA
Sbjct: 121 EADGVDTSFLVVAEGGISPFTYIIVDNKTHTRTCIHTPGSPPMVPDDLSRSSLLSALDGA 180

Query: 181 KIVYSDIRLHETALLVAQEAARQNIPMLIDAERKREGLDDLLAFASYVVCSTSFPQAKTL 240
           +IVYSD+RLHETALLVAQEAARQNIPMLIDAERKREGLDDLL FASYVVCST FP     
Sbjct: 181 RIVYSDVRLHETALLVAQEAARQNIPMLIDAERKREGLDDLLTFASYVVCSTRFP----- 240

Query: 241 FNGQEWTEAPSIPSALVSMLVRLPKLRFVIVTLGENGCIMLERSTDEKYDQMEEFEVDSL 300
              QEWT+ P+IPSALVSML+RLPKLRFVIVTLGENGCIMLERSTDE YDQMEEFEV++L
Sbjct: 241 ---QEWTDTPTIPSALVSMLLRLPKLRFVIVTLGENGCIMLERSTDETYDQMEEFEVNNL 300

Query: 301 LEVVKSRRNENINVPTSVSSVI 323
           LEVVKSRRNENINVPTSVSS +
Sbjct: 301 LEVVKSRRNENINVPTSVSSPV 313

BLAST of CSPI03G27050 vs. NCBI nr
Match: KAE8650838.1 (hypothetical protein Csa_017413 [Cucumis sativus])

HSP 1 Score: 531.6 bits (1368), Expect = 4.8e-147
Identity = 281/291 (96.56%), Postives = 282/291 (96.91%), Query Frame = 0

Query: 32  MSSSTTSSRSISFPENRVVLGVGSVAVDFLAAVASYPNPDDKIRTTSLKVQGGGNVGNAL 91
           MSSSTTSSRSISFPENRVVLGVGSVAVDFLAAVASYPNPDDKIRTTSLKVQGGGNVGNAL
Sbjct: 1   MSSSTTSSRSISFPENRVVLGVGSVAVDFLAAVASYPNPDDKIRTTSLKVQGGGNVGNAL 60

Query: 92  TSAARLGLTPRIISKVADDSQGRSIIEELEADGVDTSFLVVSEGGISPFTYIIVDNKTHT 151
           TSAARLGLTPRIISKVADDSQGRSIIEELEADGVDTSFLVVSEGGISPFTYIIVDNKTHT
Sbjct: 61  TSAARLGLTPRIISKVADDSQGRSIIEELEADGVDTSFLVVSEGGISPFTYIIVDNKTHT 120

Query: 152 RTCIHTPGSPPMVPDDLSRSSLLSALDGAKIVYSDIRLHETALLVAQEAARQNIPMLIDA 211
           RTCIHTPGSPPMVPDDLSRSSLLSALDGAKIVYSDIRLHETALLVAQEAARQNIPMLIDA
Sbjct: 121 RTCIHTPGSPPMVPDDLSRSSLLSALDGAKIVYSDIRLHETALLVAQEAARQNIPMLIDA 180

Query: 212 ERKREGLDDLLAFASYVVCSTSFPQAKTLFNGQEWTEAPSIPSALVSMLVRLPKLRFVIV 271
           ERKREGLDDLLAFASYVVCSTSFP        QEWTEAPSIPSALVSMLVRLPKLRFVIV
Sbjct: 181 ERKREGLDDLLAFASYVVCSTSFP--------QEWTEAPSIPSALVSMLVRLPKLRFVIV 240

Query: 272 TLGENGCIMLERSTDEKYDQMEEFEVDSLLEVVKSRRNENINVPTSVSSVI 323
           TLGENGCIMLERSTDEKYDQMEEFEVDSLLEVVKSRRNENINVPTSVSS +
Sbjct: 241 TLGENGCIMLERSTDEKYDQMEEFEVDSLLEVVKSRRNENINVPTSVSSPV 283

BLAST of CSPI03G27050 vs. NCBI nr
Match: XP_038888957.1 (ribokinase isoform X1 [Benincasa hispida])

HSP 1 Score: 518.5 bits (1334), Expect = 4.2e-143
Identity = 277/322 (86.02%), Postives = 290/322 (90.06%), Query Frame = 0

Query: 1   MTPLPPLLSSNKLPLHSSPTFILFSKSIRPTMSSSTTSSRSISFPENRVVLGVGSVAVDF 60
           MTPL   +SS  L LHSSPTF+ FSKS RP MSSST+SS  IS PENRVVLGVG+ +VDF
Sbjct: 1   MTPLQAFISSKTL-LHSSPTFLRFSKSTRPKMSSSTSSSVPISLPENRVVLGVGATSVDF 60

Query: 61  LAAVASYPNPDDKIRTTSLKVQGGGNVGNALTSAARLGLTPRIISKVADDSQGRSIIEEL 120
           LAAVASYPNPDDKIRTTSLKVQGGGNVGNALTSAARLGLTPRIISKVADDSQGRSIIEEL
Sbjct: 61  LAAVASYPNPDDKIRTTSLKVQGGGNVGNALTSAARLGLTPRIISKVADDSQGRSIIEEL 120

Query: 121 EADGVDTSFLVVSEGGISPFTYIIVDNKTHTRTCIHTPGSPPMVPDDLSRSSLLSALDGA 180
           EADG+DTSFLVVSEGG SPFTYIIVDNKTHTRTCIHTPGSPPMVPDDLSRSSLLSALDGA
Sbjct: 121 EADGIDTSFLVVSEGGTSPFTYIIVDNKTHTRTCIHTPGSPPMVPDDLSRSSLLSALDGA 180

Query: 181 KIVYSDIRLHETALLVAQEAARQNIPMLIDAERKREGLDDLLAFASYVVCSTSFPQAKTL 240
           + VY D+RLHETALLVAQEAARQNIPMLIDAERKREGLDDLL FASYVVCST FP     
Sbjct: 181 RFVYFDVRLHETALLVAQEAARQNIPMLIDAERKREGLDDLLLFASYVVCSTRFP----- 240

Query: 241 FNGQEWTEAPSIPSALVSMLVRLPKLRFVIVTLGENGCIMLERSTDEKYDQMEEFEVDSL 300
              QEWTEAPSIPSALVSML+RLPKL+FVIVTLGENGCIMLERS  E  DQMEEFEVDSL
Sbjct: 241 ---QEWTEAPSIPSALVSMLLRLPKLKFVIVTLGENGCIMLERSAAETSDQMEEFEVDSL 300

Query: 301 LEVVKSRRNENINVPTSVSSVI 323
           LEVV+SRR+ENINVP SVSS +
Sbjct: 301 LEVVQSRRDENINVPASVSSPV 313

BLAST of CSPI03G27050 vs. TAIR 10
Match: AT4G28706.2 (pfkB-like carbohydrate kinase family protein )

HSP 1 Score: 358.2 bits (918), Expect = 6.8e-99
Identity = 190/318 (59.75%), Postives = 240/318 (75.47%), Query Frame = 0

Query: 3   PLPPLLSSNKLPLHSSPTFILFSKSIRPTMSSSTTSSRSISFPENRVVLGVGSVAVDFLA 62
           P  PL S++ + L +S  F     S R  MSSS++       P+N +VLG G +AVDFLA
Sbjct: 8   PPLPLFSTSGIYLPASRRF----TSFRIRMSSSSSIDSVPPPPDNAIVLGCGGIAVDFLA 67

Query: 63  AVASYPNPDDKIRTTSLKVQGGGNVGNALTSAARLGLTPRIISKVADDSQGRSIIEELEA 122
            V SYP  DDKIR+TSLKVQGGGN  NALT AARLGL  R+ISKVA+DSQG+ ++EELEA
Sbjct: 68  TVDSYPQADDKIRSTSLKVQGGGNAANALTCAARLGLNSRLISKVANDSQGKGMLEELEA 127

Query: 123 DGVDTSFLVVSEGGISPFTYIIVDNKTHTRTCIHTPGSPPMVPDDLSRSSLLSALDGAKI 182
           DGVDTSF+VVS+ G SPFTYI+VDN+T TRTCIHTPG PPM+P DLS+SS+LSALD A I
Sbjct: 128 DGVDTSFIVVSKEGNSPFTYILVDNQTKTRTCIHTPGDPPMLPTDLSQSSMLSALDRASI 187

Query: 183 VYSDIRLHETALLVAQEAARQNIPMLIDAERKREGLDDLLAFASYVVCSTSFPQAKTLFN 242
           VY D+RLHETAL++A+EA+R+ IP+L+D E+KR+GLDDLL FA YVVC  +FPQ      
Sbjct: 188 VYFDVRLHETALVIAKEASRKKIPILVDTEKKRDGLDDLLPFADYVVCPENFPQT----- 247

Query: 243 GQEWTEAPSIPSALVSMLVRLPKLRFVIVTLGENGCIMLERSTDEKYDQMEEFEVDSLLE 302
              WTE  S P ALVSML+RLPKL+FVIVT GE+GC+M++R++  +  + +E +++SLLE
Sbjct: 248 ---WTEVSSTPGALVSMLLRLPKLKFVIVTSGEHGCLMVQRASKAEVFESQETDIESLLE 307

Query: 303 VVKSRRNENINVPTSVSS 321
            +K R++     PT VSS
Sbjct: 308 TLKHRKDSTTTFPTCVSS 313

BLAST of CSPI03G27050 vs. TAIR 10
Match: AT4G28706.4 (pfkB-like carbohydrate kinase family protein )

HSP 1 Score: 358.2 bits (918), Expect = 6.8e-99
Identity = 190/318 (59.75%), Postives = 240/318 (75.47%), Query Frame = 0

Query: 3   PLPPLLSSNKLPLHSSPTFILFSKSIRPTMSSSTTSSRSISFPENRVVLGVGSVAVDFLA 62
           P  PL S++ + L +S  F     S R  MSSS++       P+N +VLG G +AVDFLA
Sbjct: 8   PPLPLFSTSGIYLPASRRF----TSFRIRMSSSSSIDSVPPPPDNAIVLGCGGIAVDFLA 67

Query: 63  AVASYPNPDDKIRTTSLKVQGGGNVGNALTSAARLGLTPRIISKVADDSQGRSIIEELEA 122
            V SYP  DDKIR+TSLKVQGGGN  NALT AARLGL  R+ISKVA+DSQG+ ++EELEA
Sbjct: 68  TVDSYPQADDKIRSTSLKVQGGGNAANALTCAARLGLNSRLISKVANDSQGKGMLEELEA 127

Query: 123 DGVDTSFLVVSEGGISPFTYIIVDNKTHTRTCIHTPGSPPMVPDDLSRSSLLSALDGAKI 182
           DGVDTSF+VVS+ G SPFTYI+VDN+T TRTCIHTPG PPM+P DLS+SS+LSALD A I
Sbjct: 128 DGVDTSFIVVSKEGNSPFTYILVDNQTKTRTCIHTPGDPPMLPTDLSQSSMLSALDRASI 187

Query: 183 VYSDIRLHETALLVAQEAARQNIPMLIDAERKREGLDDLLAFASYVVCSTSFPQAKTLFN 242
           VY D+RLHETAL++A+EA+R+ IP+L+D E+KR+GLDDLL FA YVVC  +FPQ      
Sbjct: 188 VYFDVRLHETALVIAKEASRKKIPILVDTEKKRDGLDDLLPFADYVVCPENFPQT----- 247

Query: 243 GQEWTEAPSIPSALVSMLVRLPKLRFVIVTLGENGCIMLERSTDEKYDQMEEFEVDSLLE 302
              WTE  S P ALVSML+RLPKL+FVIVT GE+GC+M++R++  +  + +E +++SLLE
Sbjct: 248 ---WTEVSSTPGALVSMLLRLPKLKFVIVTSGEHGCLMVQRASKAEVFESQETDIESLLE 307

Query: 303 VVKSRRNENINVPTSVSS 321
            +K R++     PT VSS
Sbjct: 308 TLKHRKDSTTTFPTCVSS 313

BLAST of CSPI03G27050 vs. TAIR 10
Match: AT4G28706.3 (pfkB-like carbohydrate kinase family protein )

HSP 1 Score: 357.8 bits (917), Expect = 8.9e-99
Identity = 191/318 (60.06%), Postives = 241/318 (75.79%), Query Frame = 0

Query: 3   PLPPLLSSNKLPLHSSPTFILFSKSIRPTMSSSTTSSRSISFPENRVVLGVGSVAVDFLA 62
           P  PL S++ + L +S  F     S R  MSSS++       P+N +VLG G +AVDFLA
Sbjct: 8   PPLPLFSTSGIYLPASRRF----TSFRIRMSSSSSIDSVPPPPDNAIVLGCGGIAVDFLA 67

Query: 63  AVASYPNPDDKIRTTSLKVQGGGNVGNALTSAARLGLTPRIISKVADDSQGRSIIEELEA 122
            V SYP  DDKIR+TSLKVQGGGN  NALT AARLGL  R+ISKVA+DSQG+ ++EELEA
Sbjct: 68  TVDSYPQADDKIRSTSLKVQGGGNAANALTCAARLGLNSRLISKVANDSQGKGMLEELEA 127

Query: 123 DGVDTSFLVVSEGGISPFTYIIVDNKTHTRTCIHTPGSPPMVPDDLSRSSLLSALDGAKI 182
           DGVDTSF+VVS+ G SPFTYI+VDN+T TRTCIHTPG PPM+P DLS+SS+LSALD A I
Sbjct: 128 DGVDTSFIVVSKEGNSPFTYILVDNQTKTRTCIHTPGDPPMLPTDLSQSSMLSALDRASI 187

Query: 183 VYSDIRLHETALLVAQEAARQNIPMLIDAERKREGLDDLLAFASYVVCSTSFPQAKTLFN 242
           VY D+RLHETAL++A+EA+R+ IP+L+D E+KR+GLDDLL FA YVVC  +FPQ      
Sbjct: 188 VYFDVRLHETALVIAKEASRKKIPILVDTEKKRDGLDDLLPFADYVVCPENFPQT----- 247

Query: 243 GQEWTEAPSIPSALVSMLVRLPKLRFVIVTLGENGCIMLERSTDEKYDQMEEFEVDSLLE 302
              WTE  S P ALVSML+RLPKL+FVIVT GE+GC+M++R++ E ++  +E +++SLLE
Sbjct: 248 ---WTEVSSTPGALVSMLLRLPKLKFVIVTSGEHGCLMVQRASKEVFES-QETDIESLLE 307

Query: 303 VVKSRRNENINVPTSVSS 321
            +K R++     PT VSS
Sbjct: 308 TLKHRKDSTTTFPTCVSS 312

BLAST of CSPI03G27050 vs. TAIR 10
Match: AT4G28706.1 (pfkB-like carbohydrate kinase family protein )

HSP 1 Score: 355.9 bits (912), Expect = 3.4e-98
Identity = 190/318 (59.75%), Postives = 240/318 (75.47%), Query Frame = 0

Query: 3   PLPPLLSSNKLPLHSSPTFILFSKSIRPTMSSSTTSSRSISFPENRVVLGVGSVAVDFLA 62
           P  PL S++ + L +S  F       R  MSSS++       P+N +VLG G +AVDFLA
Sbjct: 8   PPLPLFSTSGIYLPASRRF------TRIRMSSSSSIDSVPPPPDNAIVLGCGGIAVDFLA 67

Query: 63  AVASYPNPDDKIRTTSLKVQGGGNVGNALTSAARLGLTPRIISKVADDSQGRSIIEELEA 122
            V SYP  DDKIR+TSLKVQGGGN  NALT AARLGL  R+ISKVA+DSQG+ ++EELEA
Sbjct: 68  TVDSYPQADDKIRSTSLKVQGGGNAANALTCAARLGLNSRLISKVANDSQGKGMLEELEA 127

Query: 123 DGVDTSFLVVSEGGISPFTYIIVDNKTHTRTCIHTPGSPPMVPDDLSRSSLLSALDGAKI 182
           DGVDTSF+VVS+ G SPFTYI+VDN+T TRTCIHTPG PPM+P DLS+SS+LSALD A I
Sbjct: 128 DGVDTSFIVVSKEGNSPFTYILVDNQTKTRTCIHTPGDPPMLPTDLSQSSMLSALDRASI 187

Query: 183 VYSDIRLHETALLVAQEAARQNIPMLIDAERKREGLDDLLAFASYVVCSTSFPQAKTLFN 242
           VY D+RLHETAL++A+EA+R+ IP+L+D E+KR+GLDDLL FA YVVC  +FPQ      
Sbjct: 188 VYFDVRLHETALVIAKEASRKKIPILVDTEKKRDGLDDLLPFADYVVCPENFPQT----- 247

Query: 243 GQEWTEAPSIPSALVSMLVRLPKLRFVIVTLGENGCIMLERSTDEKYDQMEEFEVDSLLE 302
              WTE  S P ALVSML+RLPKL+FVIVT GE+GC+M++R++ E ++  +E +++SLLE
Sbjct: 248 ---WTEVSSTPGALVSMLLRLPKLKFVIVTSGEHGCLMVQRASKEVFES-QETDIESLLE 307

Query: 303 VVKSRRNENINVPTSVSS 321
            +K R++     PT VSS
Sbjct: 308 TLKHRKDSTTTFPTCVSS 310

BLAST of CSPI03G27050 vs. TAIR 10
Match: AT5G43910.2 (pfkB-like carbohydrate kinase family protein )

HSP 1 Score: 302.8 bits (774), Expect = 3.4e-82
Identity = 154/280 (55.00%), Postives = 203/280 (72.50%), Query Frame = 0

Query: 43  SFPENRVVLGVGSVAVDFLAAVASYPNPDDKIRTTSLKVQGGGNVGNALTSAARLGLTPR 102
           + P   +VLG G + +D+L  VAS+P PD KIR TS KVQGGGN GNALT  ARLGL  R
Sbjct: 8   AIPGQPIVLGCGQLCLDYLVTVASFPIPDQKIRGTSFKVQGGGNTGNALTCVARLGLPCR 67

Query: 103 IISKVADDSQGRSIIEELEADGVDTSFLVVSEGGISPFTYIIVDNKTHTRTCIHTPGSPP 162
           I++KVADDS GR ++EELE+ GVDTSF + ++ G S F Y+IVDN+T+TRTCI+TPG PP
Sbjct: 68  ILAKVADDSHGRYMVEELESSGVDTSFCMSAKDGASHFNYVIVDNQTNTRTCIYTPGYPP 127

Query: 163 MVPDDLSRSSLLSALDGAKIVYSDIRLHETALLVAQEAARQNIPMLIDAERKREGLDDLL 222
           ++PDDL+ S LL  LDG +++Y + R  E  LL+AQ+A  +NIP+LI+AE+KR GLD+L+
Sbjct: 128 LLPDDLTESLLLDVLDGVRVLYVNGRSREAELLLAQKAHSKNIPILINAEKKRTGLDELI 187

Query: 223 AFASYVVCSTSFPQAKTLFNGQEWTEAPSIPSALVSMLVRLPKLRFVIVTLGENGCIMLE 282
             A Y +CST+FP        QEWT APS PSAL+SML+RLPKL+FVI+TLGE+GC+MLE
Sbjct: 188 DLADYAICSTNFP--------QEWTGAPSSPSALLSMLIRLPKLKFVIMTLGEHGCVMLE 247

Query: 283 RSTDEKYDQMEEFEVDSLLEVVKSRRNENINVPTSVSSVI 323
           R + E     EE ++D L E +K   +    +P   SS++
Sbjct: 248 RCSSEVSGSEEETDIDELHESLKQSTDFTSVLPVCNSSLV 279

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P500536.4e-0924.61Ketohexokinase OS=Homo sapiens OX=9606 GN=KHK PE=1 SV=2[more]
E9AD199.2e-0822.27Ribokinase OS=Leishmania major OX=5664 GN=LMJF_27_0420 PE=1 SV=1[more]
Q029741.6e-0724.61Ketohexokinase OS=Rattus norvegicus OX=10116 GN=Khk PE=1 SV=1[more]
P973283.5e-0724.61Ketohexokinase OS=Mus musculus OX=10090 GN=Khk PE=1 SV=1[more]
Q5RD713.5e-0723.66Ketohexokinase OS=Pongo abelii OX=9601 GN=KHK PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3BH878.3e-15390.68ribokinase isoform X1 OS=Cucumis melo OX=3656 GN=LOC103489824 PE=3 SV=1[more]
A0A1S3BH769.5e-14191.41ribokinase isoform X2 OS=Cucumis melo OX=3656 GN=LOC103489824 PE=3 SV=1[more]
A0A6J1HH812.9e-13781.11ribokinase-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111464044 PE=3 SV... [more]
A0A6J1HTZ43.2e-13681.11ribokinase-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111466847 PE=3 SV=1[more]
A0A6J1HRJ62.3e-13480.80ribokinase-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111466847 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_004152009.12.5e-16496.89ribokinase isoform X1 [Cucumis sativus][more]
XP_031738611.13.3e-16497.50ribokinase isoform X2 [Cucumis sativus][more]
XP_008447353.11.7e-15290.68PREDICTED: ribokinase isoform X1 [Cucumis melo][more]
KAE8650838.14.8e-14796.56hypothetical protein Csa_017413 [Cucumis sativus][more]
XP_038888957.14.2e-14386.02ribokinase isoform X1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT4G28706.26.8e-9959.75pfkB-like carbohydrate kinase family protein [more]
AT4G28706.46.8e-9959.75pfkB-like carbohydrate kinase family protein [more]
AT4G28706.38.9e-9960.06pfkB-like carbohydrate kinase family protein [more]
AT4G28706.13.4e-9859.75pfkB-like carbohydrate kinase family protein [more]
AT5G43910.23.4e-8255.00pfkB-like carbohydrate kinase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002139Ribokinase/fructokinasePRINTSPR00990RIBOKINASEcoord: 79..98
score: 35.0
coord: 269..280
score: 42.59
coord: 52..73
score: 24.75
IPR029056Ribokinase-likeGENE3D3.40.1190.20coord: 48..305
e-value: 2.9E-46
score: 160.5
IPR029056Ribokinase-likeSUPERFAMILY53613Ribokinase-likecoord: 50..286
IPR011611Carbohydrate kinase PfkBPFAMPF00294PfkBcoord: 53..289
e-value: 1.3E-20
score: 73.9
NoneNo IPR availablePANTHERPTHR42774PHOSPHOTRANSFERASE SYSTEM TRANSPORT PROTEINcoord: 39..320

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI03G27050.1CSPI03G27050.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046835 carbohydrate phosphorylation
biological_process GO:0044281 small molecule metabolic process
biological_process GO:0019252 starch biosynthetic process
molecular_function GO:0019200 carbohydrate kinase activity
molecular_function GO:0016773 phosphotransferase activity, alcohol group as acceptor
molecular_function GO:0016301 kinase activity