CSPI03G25600 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI03G25600
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionF-box/kelch-repeat protein
LocationChr3: 22975697 .. 22978244 (-)
RNA-Seq ExpressionCSPI03G25600
SyntenyCSPI03G25600
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTTCCCTTCATTTTCATCTCTCTTTACACCTTTTCTTCTTATTTTTTTTCTTCTTCTCCTTCTTCTTCTTCCCCCACTTCCATTCTCAATCCCTACCACCTCTCACGCTTCGCTTCTTTGATTTTCATCTTCGTTTTAAGATACCCATTTGCCCATTTCTTCACGGATTTCGTTTTGCTTGACTTTTCTGGTAAACCCCTTTTGTACTTTTCACTCACCGTTTTATTTTTATTTCTTTTGGTTCGATTTTATATCTGGGTTTCTTCTGTAATGATTTGTTTGGTGTTTTGGTATAGCCTGAAACTGTCTCAAGTGTTTGATTTTTGTTGTTTTAGAGCTGGATTTTTTATCTGTTTTTTGCGGTTGGATGTGTTTATTTAAAACTGGCTCTTGAGGTGTTTGTGTTCTTTGCTTGACTAAAAAGTGATTTGGGATCTTTGTGGTAGAGTTCGTTTCTTTGTTCCTTATGTAAGTGTCTCTTTGATTATTTAGCATCTGGGAATTGTTTTCTTTGAGGTGGAATGCTTCGAATTAGAAACTGATGAATTGATTGAATATTTGTTTTGGAAACATGTTCTGTTTCTTATCTTAAGGGCATATGTTTGTATTTATTGATTTTGTATTGCAGTGTTGATCGATGCATAAGGATAAAGGTTTAAAAGCTAAAGAAGGAAAAGGAAAGTTGTGTAGGTGTAATGGTTTAAGGAAGAATAAAGATATGATGTTTCGGGATTTAGTTCCCCGTAGTTCCCCACCACGATTCATCAGGGACTGTGGTGTTGGTGAATCCTCGGGTTTGAAACCTCAGGATGCAGATTATTCTATTCCTTTATTTGGTGATGAGTTGGAGCTTTCAATCTTGGCTAGGTTTCCTCAATCCGAACAATGGAAGTTGTCTTGTGTGAGCAAGAGATATTTAACTCTTGTAAGGAGTGGTGAACTGTATAGGATTAGGAAAGAAATCGGATACCAAGAGTCATCTGTTTTCATGTTAGCAAGTGGGGAGAGCAGCTGGATGATGTTTGATCGGACTTTTCAGTCGTGTCGTAGGCTTCCTGTCCTACCATCGGATACTTGCTTCTTGGATGCAGATAAGGAATCACTTTGTGCAGGCACAGATCTTTTTGTTACTGGTCGAGAACTTACAGGAGGTGCCATTTGGAGGTATCAGCTGGTTGAGAACAAATGGATCAAAGGTCCTTCTATGATCAGTCCAAGGTGTTTATTTGCATCTGCCTCCTGTGGTTCTGATGCATTTGTTGCAGGAGGGATTGCTCTAGAGTTCAGCACGGAAGGTGCTTTCGGTATGGGGATGGAATATGGACAAACTGTCTTGAACACTGTAGAAAAGTATAGCCCTGAAAGCTCATCATGGGAGCCTCTCCCAAACATGCACCGGCCGAGGAAAAAGTGCTCGGGCTGCTTCATGGATAATAAGTTCTACGTCATCGGAGGAAGAGACAAAGATGGAAACCATCTCACTTGTGGCGAAGTCTTTGACAAGGAGAAAAACTTGTGGGATTTGATTGAAAATATGCTGGAAGACACCCCAATTTCAACTTCTCAATCGCCTCCTCTTGTTGCAGTCGTGAACAATGAGCTCTACTCGCTCGAACCAGCTTCAAATGAGTTGAAGGTGTATTTGAAAGAGAGAAATGAGTGGAAGAACTTGGGTCCAGTTCCAGTCCATGCTGTTGTAAACAAAGGTTGGGGAGTTGCATTCAAGTCACTGGGGAGTGAACTTCTGGTGATTGGTGCATCGGTAGAATCATCCACAAACAATTCCATGAGCATATACACATGCACCCCAGATCCAAGGGCTGATCGATTGCAGTGGAGGCGTCTTGATGGCGGCACAAACCATCTCAGTCCATTCATTCTCAATTGTTGTGTCATGGTTGCTTGAGTAAGCCTTCATCAAATCATGTTGGGTGCCTACGGTTTTGGAATAAATAAAACGAATACCTTATTCGGTACTCGCACTTGTCATTGTCTTCGAGTTAAGTGGCTATTTTAGATTTATGTCTAGGTAATCTACAAGATAGACTTGTTGAATTTATCTTTTATGATCTTTCAGTGGTCTCCATGATTTCATCTGTTTGTTCCATCTTTCAACCCCCAACCCCTTAAAAAAGTACAAAAAAACACGATAGGAAGTAGAACTCAAACTTTATGGCTGAGACGTTATCTTCTGCAGTATTTGGTTTGTCATCTTACGATATTGTCTAGAACGAGGGTGCTTCATTGTAGGCTTCTAATTGTGTGTACGAATATGAAAATTTTCTCATTGTGCAAGTTAACTCCTTCCTCCTATCAAACAAATCTACCGC

mRNA sequence

CTTTCCCTTCATTTTCATCTCTCTTTACACCTTTTCTTCTTATTTTTTTTCTTCTTCTCCTTCTTCTTCTTCCCCCACTTCCATTCTCAATCCCTACCACCTCTCACGCTTCGCTTCTTTGATTTTCATCTTCGTTTTAAGATACCCATTTGCCCATTTCTTCACGGATTTCGTTTTGCTTGACTTTTCTGTGTTGATCGATGCATAAGGATAAAGGTTTAAAAGCTAAAGAAGGAAAAGGAAAGTTGTGTAGGTGTAATGGTTTAAGGAAGAATAAAGATATGATGTTTCGGGATTTAGTTCCCCGTAGTTCCCCACCACGATTCATCAGGGACTGTGGTGTTGGTGAATCCTCGGGTTTGAAACCTCAGGATGCAGATTATTCTATTCCTTTATTTGGTGATGAGTTGGAGCTTTCAATCTTGGCTAGGTTTCCTCAATCCGAACAATGGAAGTTGTCTTGTGTGAGCAAGAGATATTTAACTCTTGTAAGGAGTGGTGAACTGTATAGGATTAGGAAAGAAATCGGATACCAAGAGTCATCTGTTTTCATGTTAGCAAGTGGGGAGAGCAGCTGGATGATGTTTGATCGGACTTTTCAGTCGTGTCGTAGGCTTCCTGTCCTACCATCGGATACTTGCTTCTTGGATGCAGATAAGGAATCACTTTGTGCAGGCACAGATCTTTTTGTTACTGGTCGAGAACTTACAGGAGGTGCCATTTGGAGGTATCAGCTGGTTGAGAACAAATGGATCAAAGGTCCTTCTATGATCAGTCCAAGGTGTTTATTTGCATCTGCCTCCTGTGGTTCTGATGCATTTGTTGCAGGAGGGATTGCTCTAGAGTTCAGCACGGAAGGTGCTTTCGGTATGGGGATGGAATATGGACAAACTGTCTTGAACACTGTAGAAAAGTATAGCCCTGAAAGCTCATCATGGGAGCCTCTCCCAAACATGCACCGGCCGAGGAAAAAGTGCTCGGGCTGCTTCATGGATAATAAGTTCTACGTCATCGGAGGAAGAGACAAAGATGGAAACCATCTCACTTGTGGCGAAGTCTTTGACAAGGAGAAAAACTTGTGGGATTTGATTGAAAATATGCTGGAAGACACCCCAATTTCAACTTCTCAATCGCCTCCTCTTGTTGCAGTCGTGAACAATGAGCTCTACTCGCTCGAACCAGCTTCAAATGAGTTGAAGGTGTATTTGAAAGAGAGAAATGAGTGGAAGAACTTGGGTCCAGTTCCAGTCCATGCTGTTGTAAACAAAGGTTGGGGAGTTGCATTCAAGTCACTGGGGAGTGAACTTCTGGTGATTGGTGCATCGGTAGAATCATCCACAAACAATTCCATGAGCATATACACATGCACCCCAGATCCAAGGGCTGATCGATTGCAGTGGAGGCGTCTTGATGGCGGCACAAACCATCTCAGTCCATTCATTCTCAATTGTTGTGTCATGGTTGCTTGAGTAAGCCTTCATCAAATCATGTTGGGTGCCTACGGTTTTGGAATAAATAAAACGAATACCTTATTCGGTACTCGCACTTGTCATTGTCTTCGAGTTAAGTGGCTATTTTAGATTTATGTCTAGGTAATCTACAAGATAGACTTGTTGAATTTATCTTTTATGATCTTTCAGTGGTCTCCATGATTTCATCTGTTTGTTCCATCTTTCAACCCCCAACCCCTTAAAAAAGTACAAAAAAACACGATAGGAAGTAGAACTCAAACTTTATGGCTGAGACGTTATCTTCTGCAGTATTTGGTTTGTCATCTTACGATATTGTCTAGAACGAGGGTGCTTCATTGTAGGCTTCTAATTGTGTGTACGAATATGAAAATTTTCTCATTGTGCAAGTTAACTCCTTCCTCCTATCAAACAAATCTACCGC

Coding sequence (CDS)

ATGCATAAGGATAAAGGTTTAAAAGCTAAAGAAGGAAAAGGAAAGTTGTGTAGGTGTAATGGTTTAAGGAAGAATAAAGATATGATGTTTCGGGATTTAGTTCCCCGTAGTTCCCCACCACGATTCATCAGGGACTGTGGTGTTGGTGAATCCTCGGGTTTGAAACCTCAGGATGCAGATTATTCTATTCCTTTATTTGGTGATGAGTTGGAGCTTTCAATCTTGGCTAGGTTTCCTCAATCCGAACAATGGAAGTTGTCTTGTGTGAGCAAGAGATATTTAACTCTTGTAAGGAGTGGTGAACTGTATAGGATTAGGAAAGAAATCGGATACCAAGAGTCATCTGTTTTCATGTTAGCAAGTGGGGAGAGCAGCTGGATGATGTTTGATCGGACTTTTCAGTCGTGTCGTAGGCTTCCTGTCCTACCATCGGATACTTGCTTCTTGGATGCAGATAAGGAATCACTTTGTGCAGGCACAGATCTTTTTGTTACTGGTCGAGAACTTACAGGAGGTGCCATTTGGAGGTATCAGCTGGTTGAGAACAAATGGATCAAAGGTCCTTCTATGATCAGTCCAAGGTGTTTATTTGCATCTGCCTCCTGTGGTTCTGATGCATTTGTTGCAGGAGGGATTGCTCTAGAGTTCAGCACGGAAGGTGCTTTCGGTATGGGGATGGAATATGGACAAACTGTCTTGAACACTGTAGAAAAGTATAGCCCTGAAAGCTCATCATGGGAGCCTCTCCCAAACATGCACCGGCCGAGGAAAAAGTGCTCGGGCTGCTTCATGGATAATAAGTTCTACGTCATCGGAGGAAGAGACAAAGATGGAAACCATCTCACTTGTGGCGAAGTCTTTGACAAGGAGAAAAACTTGTGGGATTTGATTGAAAATATGCTGGAAGACACCCCAATTTCAACTTCTCAATCGCCTCCTCTTGTTGCAGTCGTGAACAATGAGCTCTACTCGCTCGAACCAGCTTCAAATGAGTTGAAGGTGTATTTGAAAGAGAGAAATGAGTGGAAGAACTTGGGTCCAGTTCCAGTCCATGCTGTTGTAAACAAAGGTTGGGGAGTTGCATTCAAGTCACTGGGGAGTGAACTTCTGGTGATTGGTGCATCGGTAGAATCATCCACAAACAATTCCATGAGCATATACACATGCACCCCAGATCCAAGGGCTGATCGATTGCAGTGGAGGCGTCTTGATGGCGGCACAAACCATCTCAGTCCATTCATTCTCAATTGTTGTGTCATGGTTGCTTGA

Protein sequence

MHKDKGLKAKEGKGKLCRCNGLRKNKDMMFRDLVPRSSPPRFIRDCGVGESSGLKPQDADYSIPLFGDELELSILARFPQSEQWKLSCVSKRYLTLVRSGELYRIRKEIGYQESSVFMLASGESSWMMFDRTFQSCRRLPVLPSDTCFLDADKESLCAGTDLFVTGRELTGGAIWRYQLVENKWIKGPSMISPRCLFASASCGSDAFVAGGIALEFSTEGAFGMGMEYGQTVLNTVEKYSPESSSWEPLPNMHRPRKKCSGCFMDNKFYVIGGRDKDGNHLTCGEVFDKEKNLWDLIENMLEDTPISTSQSPPLVAVVNNELYSLEPASNELKVYLKERNEWKNLGPVPVHAVVNKGWGVAFKSLGSELLVIGASVESSTNNSMSIYTCTPDPRADRLQWRRLDGGTNHLSPFILNCCVMVA*
Homology
BLAST of CSPI03G25600 vs. ExPASy Swiss-Prot
Match: Q9LI89 (F-box/kelch-repeat protein At3g27150 OS=Arabidopsis thaliana OX=3702 GN=At3g27150 PE=2 SV=1)

HSP 1 Score: 390.2 bits (1001), Expect = 3.0e-107
Identity = 198/380 (52.11%), Postives = 265/380 (69.74%), Query Frame = 0

Query: 51  SSGLKPQDADY---SIPLFGDELELSILARFPQSEQWKLSCVSKRYLTLVRSGELYRIRK 110
           S+ LKP+DADY   ++P    ELE+ ILAR P+ E WKL  ++K +  L++S E++++R+
Sbjct: 56  STCLKPKDADYCLLNVPQLVYELEVEILARVPRFEYWKLKLLNKGFSRLLKSDEIFKVRR 115

Query: 111 EIGYQESSVFMLASGESSWMMFDRTFQSCRRLPVLPSDTCFLDADKESLCAGTDLFVTGR 170
           E G  E SVFML+SG++ W MFD+ F +C++LP LPSD CFL  DKESLCAGT L VTG+
Sbjct: 116 ERGVVEPSVFMLSSGDTCWTMFDKGFGNCQKLPELPSDICFLHGDKESLCAGTHLIVTGK 175

Query: 171 ELTGGAIWRYQLVENKWIKGPSMISPRCLFASASCGSDAFVAGGIALEFSTEGAFGMGME 230
           E    A+WRY+L  +KW KGP+MI+PR LFASA+CG+  FVAGG+ +E       G G  
Sbjct: 176 EEKSIALWRYELETSKWFKGPAMITPRILFASATCGTVVFVAGGLKIE-------GNGT- 235

Query: 231 YGQTVLNTVEKYSPESSSWEPLPNMHRPRKKCSGCFMDNKFYVIGGRDKDGNHLTCGEVF 290
               V+++VEKY  ++ +W  L  MH+ RK CSGC++  KFYV+GGRD++G +LTCGE +
Sbjct: 236 --MEVVDSVEKYDSKTKTWTLLRGMHKRRKFCSGCYLRGKFYVLGGRDENGQNLTCGESY 295

Query: 291 DKEKNLWDLIENMLEDTPISTSQSPPLVAVVNNELYSLEPASNELKVYLKERNEWKNLGP 350
           D++ N W+LI ++L+D   S+ QSPPL+AVV ++LYSLE ++NEL+VY    N WK LG 
Sbjct: 296 DEKTNTWELIPDILKDMSFSSVQSPPLIAVVGDDLYSLETSANELRVYDANANSWKKLGD 355

Query: 351 VPVHAVVNKGWGVAFKSLGSELLVIGASVESSTNNSMSIYTCTPDPR-ADRLQW---RRL 410
           VPV A  N GWGVAFKSLG +LLVIGAS   S   +MS+YT  P    A++L W   +R 
Sbjct: 356 VPVRAKSNGGWGVAFKSLGDKLLVIGASAGPSRAETMSVYTSRPSANPANKLYWEESKRC 415

Query: 411 DG-GTNHLSPFILNCCVMVA 423
            G   NH   FILNCCVM+A
Sbjct: 416 CGVRFNH---FILNCCVMIA 422

BLAST of CSPI03G25600 vs. ExPASy Swiss-Prot
Match: Q9FKJ0 (F-box/kelch-repeat protein At5g60570 OS=Arabidopsis thaliana OX=3702 GN=At5g60570 PE=2 SV=1)

HSP 1 Score: 256.5 bits (654), Expect = 5.2e-67
Identity = 151/371 (40.70%), Postives = 198/371 (53.37%), Query Frame = 0

Query: 54  LKPQDADYSIPLFGDELELSILARFPQSEQWKLSCVSKRYLTLVRSGELYRIRKEIGYQE 113
           L+   +D  +P   D++ L+ LA  P+S+   LSCV+K+Y  L+ SG L+ +RKE+G  E
Sbjct: 41  LRLGSSDSVLPGLIDDVALNCLAWVPRSDYPSLSCVNKKYNKLINSGHLFALRKELGIVE 100

Query: 114 SSVFMLASGESSWMMFDRTFQSCRRLPVLPSDTCFLDADKESLCAGTDLFVTGRELTGGA 173
             VFM+      W+MF    +    LP +P D CF  ADKESL    +L V GREL   A
Sbjct: 101 YLVFMVCD-PRGWLMFSPMKKKWMVLPKMPCDECFNHADKESLAVDDELLVFGRELFQFA 160

Query: 174 IWRYQLVENKWIKGPSMISPRCLFASASCGSDAFVAGGIALEFSTEGAFGMGMEYGQTVL 233
           IW+Y L    W+K   M  PRCLFAS S G  A VAG              G +    +L
Sbjct: 161 IWKYSLRSRCWVKCEGMHRPRCLFASGSLGGIAIVAG--------------GTDMNGNIL 220

Query: 234 NTVEKYSPESSSWEPLPNMHRPRKKCSGCFMDNKFYVIGGRDKDGNHLTCGEVFDKEKNL 293
            + E Y   S  WE LPNMH PR+ CSG FMD KFYVIGG       +T GE FD E   
Sbjct: 221 ASAELYDSSSGRWEMLPNMHSPRRLCSGFFMDGKFYVIGGMSSPNVSVTFGEEFDLETRK 280

Query: 294 WDLIENMLEDTPISTSQSPPLVAVVNNELYSLEPASNELKVYLKERNEWKNLGPVPVHAV 353
           W  IE M  +     +Q+PPLV VVNNEL++LE ++N +K Y K +N+W+ +G +P    
Sbjct: 281 WRKIEGMYPNVN-RAAQAPPLVVVVNNELFTLEYSTNMVKKYDKVKNKWEVMGRLPPMVD 340

Query: 354 VNKGWGVAFKSLGSELLVI----GASVESSTNNSMSIYTCTPDPRADRLQWRRLDGGTNH 413
            + GWG+AFK  G +LLV     G   E    NS    +   D     L W+ L G   +
Sbjct: 341 SSNGWGLAFKPCGDQLLVFCGQRGPHGEGIVVNSWCPKSGAKD---GNLDWKVL-GVKEN 391

Query: 414 LSPFILNCCVM 421
           +  F+ NC VM
Sbjct: 401 VGVFVYNCAVM 391

BLAST of CSPI03G25600 vs. ExPASy Swiss-Prot
Match: Q8L736 (F-box/kelch-repeat protein SKIP11 OS=Arabidopsis thaliana OX=3702 GN=SKIP11 PE=1 SV=2)

HSP 1 Score: 231.9 bits (590), Expect = 1.4e-59
Identity = 138/378 (36.51%), Postives = 202/378 (53.44%), Query Frame = 0

Query: 52  SGLKPQDADYSIPLFGDELELSILARFPQSEQWKLSCVSKRYLTLVRSGELYRIRKEIGY 111
           +G    D+   I   G +  +  L R  +S+   ++ +++ + +LV+SGE+YR+R++ G+
Sbjct: 107 NGGDSSDSHSLINEIGRDNSIDCLIRCSRSDYGSIASLNRNFRSLVKSGEIYRLRRQNGF 166

Query: 112 QESSVFMLASGESSWMMFDRTFQSCRRLPVLPSDTCFLDADKESLCAGTDLFVTGR-ELT 171
            E  V+  +     W+ FD   +   +LP +PS   F+ ADKESL  GTDL V G+ + +
Sbjct: 167 VEHWVY-FSCQLLEWVAFDPVERRWMQLPTMPSSVTFMCADKESLAVGTDLLVLGKDDFS 226

Query: 172 GGAIWRYQLVENKWIKGPSMISPRCLFASASCGSDAFVAGGIALEFSTEGAFGMGMEYGQ 231
              I+RY L+ N W  G  M SPRCLF SAS G  A  AGG      ++G          
Sbjct: 227 SHVIYRYSLLTNSWSSGMKMNSPRCLFGSASLGEIAIFAGGC----DSQG---------- 286

Query: 232 TVLNTVEKYSPESSSWEPLPNMHRPRKKCSGCFMDNKFYVIGG-RDKDGNHLTCGEVFDK 291
            +L+  E Y+ E  +W  LP M++PRK CSG FMD KFYVIGG    D   LTCGE +D 
Sbjct: 287 KILDFAEMYNSELQTWITLPRMNKPRKMCSGVFMDGKFYVIGGIGGADSKGLTCGEEYDL 346

Query: 292 EKNLWDLIENM------LEDTPIS-TSQSPPLVAVVNNELYSLEPASNELKVYLKERNEW 351
           E   W  I ++       +   +S  +++PPLVAVVNN+LY+ + A  E++ Y KE  +W
Sbjct: 347 ETKKWTQIPDLSPPRSRADQADMSPAAEAPPLVAVVNNQLYAADHADMEVRKYDKENKKW 406

Query: 352 KNLGPVPVHAVVNKGWGVAFKSLGSELLVIGASVESSTNNSMSIYTCTPDPRADRLQWRR 411
             +G +P  A    GWG+AF++ G  L+VIG   + S    + + +  P       QW  
Sbjct: 407 LTVGRLPERAGSVNGWGLAFRACGERLIVIGGP-KCSGGGFIELNSWIPSDGGPP-QWTL 465

Query: 412 LDGGTNHLSPFILNCCVM 421
           LD    H   F+ NC VM
Sbjct: 467 LD--RKHSPTFVYNCAVM 465

BLAST of CSPI03G25600 vs. ExPASy Swiss-Prot
Match: Q84M94 (F-box/kelch-repeat protein At1g26930 OS=Arabidopsis thaliana OX=3702 GN=At1g26930 PE=2 SV=1)

HSP 1 Score: 219.2 bits (557), Expect = 9.1e-56
Identity = 135/386 (34.97%), Postives = 196/386 (50.78%), Query Frame = 0

Query: 45  DCGVGESSGLKPQDADYSIPLFGDELELSILARFPQSEQWKLSCVSKRYLTLVRSGELYR 104
           D   GE +G    D+   IP    +  LS L R  +++   ++ V++   +L+RSGE+YR
Sbjct: 55  DSSEGEDNG-SSSDSGTLIPGMNRDDSLSCLIRCSRADYCSIASVNRSLRSLIRSGEIYR 114

Query: 105 IRKEIGYQESSVFMLASGESSWMMFDRTFQSCRRLPVLPSDTCFLDADKESLCAGTDLFV 164
           +R+  G  E  V+  +   + W  FD   +    LP +P + CF  ADKESL  GTDL V
Sbjct: 115 LRRLQGTLEHWVY-FSCHLNEWEAFDPRSKRWMHLPSMPQNECFRYADKESLAVGTDLLV 174

Query: 165 TGRELTGGAIWRYQLVENKWIKGPSMISPRCLFASASCGSDAFVAGGIALEFSTEGAFGM 224
            G E++   I+RY L+ N W    SM  PRCLF SAS G  A +AG              
Sbjct: 175 FGWEVSSYVIYRYSLLTNSWSTAKSMNMPRCLFGSASYGEIAVLAG-------------- 234

Query: 225 GMEYGQTVLNTVEKYSPESSSWEPLPNMHRPRKKCSGCFMDNKFYVIG----GRDKDGNH 284
           G +    +L+T E Y+ E  +W  LP M++ RK CSG FMD KFYVIG    G + +   
Sbjct: 235 GCDSSGRILDTAELYNYEDQTWLVLPGMNKRRKMCSGVFMDGKFYVIGGIGVGEENEPKV 294

Query: 285 LTCGEVFDKEKNLWDLIENMLE------DTPISTSQSPPLVAVVNNELYSLEPASNELKV 344
           LTCGE FD +   W  I  M        +   + + +PPLVAVVN++LY+ + A   ++ 
Sbjct: 295 LTCGEEFDLKTRKWTEIPEMSPPRSNQGNGMSAAAMAPPLVAVVNDQLYAADHAGMAVRR 354

Query: 345 YLKERNEWKNLGPVPVHAVVNKGWGVAFKSLGSELLVIGASVESSTNNSMSIYTCTPDPR 404
           Y KE+  W  +G +P  A    GWG+AF++ G  ++VIG   ++     + + +  P   
Sbjct: 355 YDKEKRVWNKVGNLPEQAGSMNGWGLAFRACGDRIIVIGGP-KAPGEGFIELNSWVPSVT 414

Query: 405 ADRLQWRRLDGGTNHLSPFILNCCVM 421
               +W  L  G      F+ NC VM
Sbjct: 415 TP--EWHLL--GKKQSVNFVYNCAVM 419

BLAST of CSPI03G25600 vs. ExPASy Swiss-Prot
Match: Q9CA63 (F-box/kelch-repeat protein At1g74510 OS=Arabidopsis thaliana OX=3702 GN=At1g74510 PE=2 SV=1)

HSP 1 Score: 214.9 bits (546), Expect = 1.7e-54
Identity = 123/364 (33.79%), Postives = 185/364 (50.82%), Query Frame = 0

Query: 72  LSILARFPQSEQWKLSCVSKRYLTLVRSGELYRIRKEIGYQESSVFMLASGESSWMMFDR 131
           L+ LA    S+   ++  ++ + +L++  ELYR+R+  G  E  ++  +     W  +D 
Sbjct: 105 LNCLAHCSLSDFGSIASTNRTFRSLIKDSELYRLRRAKGIVEHWIY-FSCRLLEWEAYDP 164

Query: 132 TFQSCRRLPVLPSDTCFLDADKESLCAGTDLFVTGRELTGGAIWRYQLVENKWIKGPSMI 191
                 R+P +  + CF+ +DKESL  GT+L V G+E+    I+RY ++ N W  G  M 
Sbjct: 165 NGDRWLRVPKMTFNECFMCSDKESLAVGTELLVFGKEIMSHVIYRYSILTNTWTSGMQMN 224

Query: 192 SPRCLFASASCGSDAFVAGGIALEFSTEGAFGMGMEYGQTVLNTVEKYSPESSSWEPLPN 251
            PRCLF SAS G  A +AG              G +    +L++ E Y+ E+  W  +P+
Sbjct: 225 VPRCLFGSASLGEIAVIAG--------------GCDPRGRILSSAELYNSETGEWTVIPS 284

Query: 252 MHRPRKKCSGCFMDNKFYVIGG-RDKDGNHLTCGEVFDKEKNLWDLIENMLED------- 311
           M++ RK CS  FMD  FY IGG  + +   L CGEV+D +K  W LI NML +       
Sbjct: 285 MNKARKMCSSVFMDGNFYCIGGIGEGNSKMLLCGEVYDLKKKTWTLIPNMLPERSSGGGG 344

Query: 312 -------TPISTSQSPPLVAVVNNELYSLEPASNELKVYLKERNEWKNLGPVPVHAVVNK 371
                     + S++PPLVAVV +ELY+   A  E+K Y K  N W  +G +P  A    
Sbjct: 345 DQAKEIAAATAASEAPPLVAVVKDELYAANYAQQEVKKYDKRLNVWNKVGNLPERASSMN 404

Query: 372 GWGVAFKSLGSELLVIGASVESSTNNSMSIYTCTPDPRADRLQWRRLDGGTNHLSPFILN 421
           GWG+AF++ G +L+V+G    +     + I  C P     +L WR L   +     F+ N
Sbjct: 405 GWGMAFRACGDQLVVVGGP-RAIGGGFIEINACVPS-EGTQLHWRVL--ASKPSGNFVYN 449

BLAST of CSPI03G25600 vs. ExPASy TrEMBL
Match: A0A0A0L8A9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G586950 PE=4 SV=1)

HSP 1 Score: 879.4 bits (2271), Expect = 6.0e-252
Identity = 422/422 (100.00%), Postives = 422/422 (100.00%), Query Frame = 0

Query: 1   MHKDKGLKAKEGKGKLCRCNGLRKNKDMMFRDLVPRSSPPRFIRDCGVGESSGLKPQDAD 60
           MHKDKGLKAKEGKGKLCRCNGLRKNKDMMFRDLVPRSSPPRFIRDCGVGESSGLKPQDAD
Sbjct: 1   MHKDKGLKAKEGKGKLCRCNGLRKNKDMMFRDLVPRSSPPRFIRDCGVGESSGLKPQDAD 60

Query: 61  YSIPLFGDELELSILARFPQSEQWKLSCVSKRYLTLVRSGELYRIRKEIGYQESSVFMLA 120
           YSIPLFGDELELSILARFPQSEQWKLSCVSKRYLTLVRSGELYRIRKEIGYQESSVFMLA
Sbjct: 61  YSIPLFGDELELSILARFPQSEQWKLSCVSKRYLTLVRSGELYRIRKEIGYQESSVFMLA 120

Query: 121 SGESSWMMFDRTFQSCRRLPVLPSDTCFLDADKESLCAGTDLFVTGRELTGGAIWRYQLV 180
           SGESSWMMFDRTFQSCRRLPVLPSDTCFLDADKESLCAGTDLFVTGRELTGGAIWRYQLV
Sbjct: 121 SGESSWMMFDRTFQSCRRLPVLPSDTCFLDADKESLCAGTDLFVTGRELTGGAIWRYQLV 180

Query: 181 ENKWIKGPSMISPRCLFASASCGSDAFVAGGIALEFSTEGAFGMGMEYGQTVLNTVEKYS 240
           ENKWIKGPSMISPRCLFASASCGSDAFVAGGIALEFSTEGAFGMGMEYGQTVLNTVEKYS
Sbjct: 181 ENKWIKGPSMISPRCLFASASCGSDAFVAGGIALEFSTEGAFGMGMEYGQTVLNTVEKYS 240

Query: 241 PESSSWEPLPNMHRPRKKCSGCFMDNKFYVIGGRDKDGNHLTCGEVFDKEKNLWDLIENM 300
           PESSSWEPLPNMHRPRKKCSGCFMDNKFYVIGGRDKDGNHLTCGEVFDKEKNLWDLIENM
Sbjct: 241 PESSSWEPLPNMHRPRKKCSGCFMDNKFYVIGGRDKDGNHLTCGEVFDKEKNLWDLIENM 300

Query: 301 LEDTPISTSQSPPLVAVVNNELYSLEPASNELKVYLKERNEWKNLGPVPVHAVVNKGWGV 360
           LEDTPISTSQSPPLVAVVNNELYSLEPASNELKVYLKERNEWKNLGPVPVHAVVNKGWGV
Sbjct: 301 LEDTPISTSQSPPLVAVVNNELYSLEPASNELKVYLKERNEWKNLGPVPVHAVVNKGWGV 360

Query: 361 AFKSLGSELLVIGASVESSTNNSMSIYTCTPDPRADRLQWRRLDGGTNHLSPFILNCCVM 420
           AFKSLGSELLVIGASVESSTNNSMSIYTCTPDPRADRLQWRRLDGGTNHLSPFILNCCVM
Sbjct: 361 AFKSLGSELLVIGASVESSTNNSMSIYTCTPDPRADRLQWRRLDGGTNHLSPFILNCCVM 420

Query: 421 VA 423
           VA
Sbjct: 421 VA 422

BLAST of CSPI03G25600 vs. ExPASy TrEMBL
Match: A0A1S3BP52 (F-box/kelch-repeat protein At3g27150 OS=Cucumis melo OX=3656 GN=LOC103491782 PE=4 SV=1)

HSP 1 Score: 853.6 bits (2204), Expect = 3.5e-244
Identity = 413/423 (97.64%), Postives = 415/423 (98.11%), Query Frame = 0

Query: 1   MHKDKGLKAK-EGKGKLCRCNGLRKNKDMMFRDLVPRSSPPRFIRDCGVGESSGLKPQDA 60
           MHKDKGLKAK EGKGKLCRCNGLRKNKDMMF DLVPRSS PRFIRDCGVGESS LKPQDA
Sbjct: 1   MHKDKGLKAKEEGKGKLCRCNGLRKNKDMMFGDLVPRSSAPRFIRDCGVGESSCLKPQDA 60

Query: 61  DYSIPLFGDELELSILARFPQSEQWKLSCVSKRYLTLVRSGELYRIRKEIGYQESSVFML 120
           DYSIPLF DELELSILARFPQSEQWKLSCVSKRYLTLV+SGELYRIRKEIGYQESSVFML
Sbjct: 61  DYSIPLFADELELSILARFPQSEQWKLSCVSKRYLTLVKSGELYRIRKEIGYQESSVFML 120

Query: 121 ASGESSWMMFDRTFQSCRRLPVLPSDTCFLDADKESLCAGTDLFVTGRELTGGAIWRYQL 180
           ASGESSWMMFDRTFQSCRRLPVLPSDTCFLDADKESLCAGTDL VTGRELTGGAIWRYQL
Sbjct: 121 ASGESSWMMFDRTFQSCRRLPVLPSDTCFLDADKESLCAGTDLIVTGRELTGGAIWRYQL 180

Query: 181 VENKWIKGPSMISPRCLFASASCGSDAFVAGGIALEFSTEGAFGMGMEYGQTVLNTVEKY 240
           VENKW KGPSMISPRCLFASASCGSDAFVAGGIALEFSTEGAFGMGMEYGQTVLNTVEKY
Sbjct: 181 VENKWTKGPSMISPRCLFASASCGSDAFVAGGIALEFSTEGAFGMGMEYGQTVLNTVEKY 240

Query: 241 SPESSSWEPLPNMHRPRKKCSGCFMDNKFYVIGGRDKDGNHLTCGEVFDKEKNLWDLIEN 300
           SPESSSWEPLPNMHR RKKCSGCFMDNKFYVIGGRDKDGNHLTCGEVFDKEKNLWDLIEN
Sbjct: 241 SPESSSWEPLPNMHRSRKKCSGCFMDNKFYVIGGRDKDGNHLTCGEVFDKEKNLWDLIEN 300

Query: 301 MLEDTPISTSQSPPLVAVVNNELYSLEPASNELKVYLKERNEWKNLGPVPVHAVVNKGWG 360
           MLEDTPISTSQSPPLVAVVNNELYSLEPASNELKVYLKERNEWKNLGPVPVHAVVNKGWG
Sbjct: 301 MLEDTPISTSQSPPLVAVVNNELYSLEPASNELKVYLKERNEWKNLGPVPVHAVVNKGWG 360

Query: 361 VAFKSLGSELLVIGASVESSTNNSMSIYTCTPDPRADRLQWRRLDGGTNHLSPFILNCCV 420
           VAFKSLGSELLVIGASVESSTNNSMSIYTCTPDPRADRLQW+RLDGGTNHLSPFILNCCV
Sbjct: 361 VAFKSLGSELLVIGASVESSTNNSMSIYTCTPDPRADRLQWKRLDGGTNHLSPFILNCCV 420

Query: 421 MVA 423
           MVA
Sbjct: 421 MVA 423

BLAST of CSPI03G25600 vs. ExPASy TrEMBL
Match: A0A5A7THW5 (F-box/kelch-repeat protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold108G001130 PE=4 SV=1)

HSP 1 Score: 853.6 bits (2204), Expect = 3.5e-244
Identity = 413/423 (97.64%), Postives = 415/423 (98.11%), Query Frame = 0

Query: 1   MHKDKGLKAK-EGKGKLCRCNGLRKNKDMMFRDLVPRSSPPRFIRDCGVGESSGLKPQDA 60
           MHKDKGLKAK EGKGKLCRCNGLRKNKDMMF DLVPRSS PRFIRDCGVGESS LKPQDA
Sbjct: 1   MHKDKGLKAKEEGKGKLCRCNGLRKNKDMMFGDLVPRSSAPRFIRDCGVGESSCLKPQDA 60

Query: 61  DYSIPLFGDELELSILARFPQSEQWKLSCVSKRYLTLVRSGELYRIRKEIGYQESSVFML 120
           DYSIPLF DELELSILARFPQSEQWKLSCVSKRYLTLV+SGELYRIRKEIGYQESSVFML
Sbjct: 61  DYSIPLFADELELSILARFPQSEQWKLSCVSKRYLTLVKSGELYRIRKEIGYQESSVFML 120

Query: 121 ASGESSWMMFDRTFQSCRRLPVLPSDTCFLDADKESLCAGTDLFVTGRELTGGAIWRYQL 180
           ASGESSWMMFDRTFQSCRRLPVLPSDTCFLDADKESLCAGTDL VTGRELTGGAIWRYQL
Sbjct: 121 ASGESSWMMFDRTFQSCRRLPVLPSDTCFLDADKESLCAGTDLIVTGRELTGGAIWRYQL 180

Query: 181 VENKWIKGPSMISPRCLFASASCGSDAFVAGGIALEFSTEGAFGMGMEYGQTVLNTVEKY 240
           VENKW KGPSMISPRCLFASASCGSDAFVAGGIALEFSTEGAFGMGMEYGQTVLNTVEKY
Sbjct: 181 VENKWTKGPSMISPRCLFASASCGSDAFVAGGIALEFSTEGAFGMGMEYGQTVLNTVEKY 240

Query: 241 SPESSSWEPLPNMHRPRKKCSGCFMDNKFYVIGGRDKDGNHLTCGEVFDKEKNLWDLIEN 300
           SPESSSWEPLPNMHR RKKCSGCFMDNKFYVIGGRDKDGNHLTCGEVFDKEKNLWDLIEN
Sbjct: 241 SPESSSWEPLPNMHRSRKKCSGCFMDNKFYVIGGRDKDGNHLTCGEVFDKEKNLWDLIEN 300

Query: 301 MLEDTPISTSQSPPLVAVVNNELYSLEPASNELKVYLKERNEWKNLGPVPVHAVVNKGWG 360
           MLEDTPISTSQSPPLVAVVNNELYSLEPASNELKVYLKERNEWKNLGPVPVHAVVNKGWG
Sbjct: 301 MLEDTPISTSQSPPLVAVVNNELYSLEPASNELKVYLKERNEWKNLGPVPVHAVVNKGWG 360

Query: 361 VAFKSLGSELLVIGASVESSTNNSMSIYTCTPDPRADRLQWRRLDGGTNHLSPFILNCCV 420
           VAFKSLGSELLVIGASVESSTNNSMSIYTCTPDPRADRLQW+RLDGGTNHLSPFILNCCV
Sbjct: 361 VAFKSLGSELLVIGASVESSTNNSMSIYTCTPDPRADRLQWKRLDGGTNHLSPFILNCCV 420

Query: 421 MVA 423
           MVA
Sbjct: 421 MVA 423

BLAST of CSPI03G25600 vs. ExPASy TrEMBL
Match: A0A6J1F4C0 (F-box/kelch-repeat protein At3g27150-like OS=Cucurbita moschata OX=3662 GN=LOC111441987 PE=4 SV=1)

HSP 1 Score: 738.8 bits (1906), Expect = 1.3e-209
Identity = 364/423 (86.05%), Postives = 380/423 (89.83%), Query Frame = 0

Query: 1   MHKDKGLKAKEGKGKLCRCNGLRKNKDMMFRDL-VPRSSPPRFIRDCGVGESSGLKPQDA 60
           M+KDKGL+        C     +KN     RDL VPR+S PRF  D GVGESSGLK QDA
Sbjct: 1   MYKDKGLEG-------CVSGSRKKNNR---RDLTVPRNSAPRFALDYGVGESSGLKSQDA 60

Query: 61  DYSIPLFGDELELSILARFPQSEQWKLSCVSKRYLTLVRSGELYRIRKEIGYQESSVFML 120
           DYSIPLFGDELELSILARFP+SEQWKLSCVSKRYL +V+SGELY+IRK IGY+E SVFML
Sbjct: 61  DYSIPLFGDELELSILARFPKSEQWKLSCVSKRYLAIVKSGELYKIRKAIGYKEPSVFML 120

Query: 121 ASGESSWMMFDRTFQSCRRLPVLPSDTCFLDADKESLCAGTDLFVTGRELTGGAIWRYQL 180
           ASGESSWMMFDRTFQSCRRLP+LPSD+CFLDADKESLCAGTDL VTGRELTGGAIWRYQL
Sbjct: 121 ASGESSWMMFDRTFQSCRRLPILPSDSCFLDADKESLCAGTDLIVTGRELTGGAIWRYQL 180

Query: 181 VENKWIKGPSMISPRCLFASASCGSDAFVAGGIALEFSTEGAFGMGMEYGQTVLNTVEKY 240
           VENKWI GPSMISPRCLFASAS GS+AFVAGGIALEFSTEGAFGMGMEYGQTVLNTVEKY
Sbjct: 181 VENKWINGPSMISPRCLFASASSGSNAFVAGGIALEFSTEGAFGMGMEYGQTVLNTVEKY 240

Query: 241 SPESSSWEPLPNMHRPRKKCSGCFMDNKFYVIGGRDKDGNHLTCGEVFDKEKNLWDLIEN 300
           +PES  WEPLPNMHR RKKCSGCFMDNKFYVIGGRD DGNHLTCGEVFD EKN WDLIEN
Sbjct: 241 NPESLLWEPLPNMHRSRKKCSGCFMDNKFYVIGGRDNDGNHLTCGEVFDTEKNSWDLIEN 300

Query: 301 MLEDTPISTSQSPPLVAVVNNELYSLEPASNELKVYLKERNEWKNLGPVPVHAVVNKGWG 360
           MLED PISTSQSPPLVAVVNNELYSLEP+SNELKVYLK RNEWKNLGPVPV AVVNKGWG
Sbjct: 301 MLEDAPISTSQSPPLVAVVNNELYSLEPSSNELKVYLKGRNEWKNLGPVPVLAVVNKGWG 360

Query: 361 VAFKSLGSELLVIGASVESSTNNSMSIYTCTPDPRADRLQWRRLDGGTNHLSPFILNCCV 420
           VAFKSLG ELLVIGASVESS N+SMSIYTC+PDPRADRLQW RLD GTNHLSPFILNCCV
Sbjct: 361 VAFKSLGDELLVIGASVESSANDSMSIYTCSPDPRADRLQWTRLDCGTNHLSPFILNCCV 413

Query: 421 MVA 423
           MVA
Sbjct: 421 MVA 413

BLAST of CSPI03G25600 vs. ExPASy TrEMBL
Match: A0A6J1J5B8 (F-box/kelch-repeat protein At3g27150-like OS=Cucurbita maxima OX=3661 GN=LOC111481490 PE=4 SV=1)

HSP 1 Score: 736.9 bits (1901), Expect = 4.8e-209
Identity = 363/423 (85.82%), Postives = 380/423 (89.83%), Query Frame = 0

Query: 1   MHKDKGLKAKEGKGKLCRCNGLRKNKDMMFRDL-VPRSSPPRFIRDCGVGESSGLKPQDA 60
           M+KDKGL+        C     +KN     RDL VPR+S PRF   CGVGESSGLK QDA
Sbjct: 1   MYKDKGLEG-------CVSGSRKKNNR---RDLTVPRNSAPRFALGCGVGESSGLKSQDA 60

Query: 61  DYSIPLFGDELELSILARFPQSEQWKLSCVSKRYLTLVRSGELYRIRKEIGYQESSVFML 120
           DYSIPLFGDELELSILARFP+SEQWKLSCVSKRYL +V+SGELY+IRK IGY+E SVFML
Sbjct: 61  DYSIPLFGDELELSILARFPKSEQWKLSCVSKRYLAIVKSGELYKIRKAIGYKEPSVFML 120

Query: 121 ASGESSWMMFDRTFQSCRRLPVLPSDTCFLDADKESLCAGTDLFVTGRELTGGAIWRYQL 180
           ASGESSWMMFD+TFQSCRRLP+LPSD+CFLDADKESLCAGTDL VTGRELTGGAIWRYQL
Sbjct: 121 ASGESSWMMFDQTFQSCRRLPILPSDSCFLDADKESLCAGTDLIVTGRELTGGAIWRYQL 180

Query: 181 VENKWIKGPSMISPRCLFASASCGSDAFVAGGIALEFSTEGAFGMGMEYGQTVLNTVEKY 240
           VENKWI GPSMISPRCLFASAS GS+AFVAGGIALEFSTEGAFGMGMEYGQTVLNTVEKY
Sbjct: 181 VENKWINGPSMISPRCLFASASSGSNAFVAGGIALEFSTEGAFGMGMEYGQTVLNTVEKY 240

Query: 241 SPESSSWEPLPNMHRPRKKCSGCFMDNKFYVIGGRDKDGNHLTCGEVFDKEKNLWDLIEN 300
           +PES  WEPLPNM R RKKCSGCFMDNKFYVIGGRD DGNHLTCGEVFD EKN WDLIEN
Sbjct: 241 NPESLLWEPLPNMLRSRKKCSGCFMDNKFYVIGGRDNDGNHLTCGEVFDTEKNSWDLIEN 300

Query: 301 MLEDTPISTSQSPPLVAVVNNELYSLEPASNELKVYLKERNEWKNLGPVPVHAVVNKGWG 360
           MLED PISTSQSPPLVAVVNNELYSLEP+SNELKVYLK RNEWKNLGPVPV AVVNKGWG
Sbjct: 301 MLEDAPISTSQSPPLVAVVNNELYSLEPSSNELKVYLKGRNEWKNLGPVPVLAVVNKGWG 360

Query: 361 VAFKSLGSELLVIGASVESSTNNSMSIYTCTPDPRADRLQWRRLDGGTNHLSPFILNCCV 420
           VAFKSLG ELLVIGASVESS N+SMSIYTC+PDPRADRLQWRRLD GTNHLSPFILNCCV
Sbjct: 361 VAFKSLGDELLVIGASVESSANDSMSIYTCSPDPRADRLQWRRLDCGTNHLSPFILNCCV 413

Query: 421 MVA 423
           MVA
Sbjct: 421 MVA 413

BLAST of CSPI03G25600 vs. NCBI nr
Match: XP_004145670.1 (F-box/kelch-repeat protein At3g27150 [Cucumis sativus] >KGN58185.1 hypothetical protein Csa_017472 [Cucumis sativus])

HSP 1 Score: 879.4 bits (2271), Expect = 1.2e-251
Identity = 422/422 (100.00%), Postives = 422/422 (100.00%), Query Frame = 0

Query: 1   MHKDKGLKAKEGKGKLCRCNGLRKNKDMMFRDLVPRSSPPRFIRDCGVGESSGLKPQDAD 60
           MHKDKGLKAKEGKGKLCRCNGLRKNKDMMFRDLVPRSSPPRFIRDCGVGESSGLKPQDAD
Sbjct: 1   MHKDKGLKAKEGKGKLCRCNGLRKNKDMMFRDLVPRSSPPRFIRDCGVGESSGLKPQDAD 60

Query: 61  YSIPLFGDELELSILARFPQSEQWKLSCVSKRYLTLVRSGELYRIRKEIGYQESSVFMLA 120
           YSIPLFGDELELSILARFPQSEQWKLSCVSKRYLTLVRSGELYRIRKEIGYQESSVFMLA
Sbjct: 61  YSIPLFGDELELSILARFPQSEQWKLSCVSKRYLTLVRSGELYRIRKEIGYQESSVFMLA 120

Query: 121 SGESSWMMFDRTFQSCRRLPVLPSDTCFLDADKESLCAGTDLFVTGRELTGGAIWRYQLV 180
           SGESSWMMFDRTFQSCRRLPVLPSDTCFLDADKESLCAGTDLFVTGRELTGGAIWRYQLV
Sbjct: 121 SGESSWMMFDRTFQSCRRLPVLPSDTCFLDADKESLCAGTDLFVTGRELTGGAIWRYQLV 180

Query: 181 ENKWIKGPSMISPRCLFASASCGSDAFVAGGIALEFSTEGAFGMGMEYGQTVLNTVEKYS 240
           ENKWIKGPSMISPRCLFASASCGSDAFVAGGIALEFSTEGAFGMGMEYGQTVLNTVEKYS
Sbjct: 181 ENKWIKGPSMISPRCLFASASCGSDAFVAGGIALEFSTEGAFGMGMEYGQTVLNTVEKYS 240

Query: 241 PESSSWEPLPNMHRPRKKCSGCFMDNKFYVIGGRDKDGNHLTCGEVFDKEKNLWDLIENM 300
           PESSSWEPLPNMHRPRKKCSGCFMDNKFYVIGGRDKDGNHLTCGEVFDKEKNLWDLIENM
Sbjct: 241 PESSSWEPLPNMHRPRKKCSGCFMDNKFYVIGGRDKDGNHLTCGEVFDKEKNLWDLIENM 300

Query: 301 LEDTPISTSQSPPLVAVVNNELYSLEPASNELKVYLKERNEWKNLGPVPVHAVVNKGWGV 360
           LEDTPISTSQSPPLVAVVNNELYSLEPASNELKVYLKERNEWKNLGPVPVHAVVNKGWGV
Sbjct: 301 LEDTPISTSQSPPLVAVVNNELYSLEPASNELKVYLKERNEWKNLGPVPVHAVVNKGWGV 360

Query: 361 AFKSLGSELLVIGASVESSTNNSMSIYTCTPDPRADRLQWRRLDGGTNHLSPFILNCCVM 420
           AFKSLGSELLVIGASVESSTNNSMSIYTCTPDPRADRLQWRRLDGGTNHLSPFILNCCVM
Sbjct: 361 AFKSLGSELLVIGASVESSTNNSMSIYTCTPDPRADRLQWRRLDGGTNHLSPFILNCCVM 420

Query: 421 VA 423
           VA
Sbjct: 421 VA 422

BLAST of CSPI03G25600 vs. NCBI nr
Match: XP_008450091.1 (PREDICTED: F-box/kelch-repeat protein At3g27150 [Cucumis melo] >XP_008450092.1 PREDICTED: F-box/kelch-repeat protein At3g27150 [Cucumis melo] >KAA0042890.1 F-box/kelch-repeat protein [Cucumis melo var. makuwa] >TYK05293.1 F-box/kelch-repeat protein [Cucumis melo var. makuwa])

HSP 1 Score: 853.6 bits (2204), Expect = 7.3e-244
Identity = 413/423 (97.64%), Postives = 415/423 (98.11%), Query Frame = 0

Query: 1   MHKDKGLKAK-EGKGKLCRCNGLRKNKDMMFRDLVPRSSPPRFIRDCGVGESSGLKPQDA 60
           MHKDKGLKAK EGKGKLCRCNGLRKNKDMMF DLVPRSS PRFIRDCGVGESS LKPQDA
Sbjct: 1   MHKDKGLKAKEEGKGKLCRCNGLRKNKDMMFGDLVPRSSAPRFIRDCGVGESSCLKPQDA 60

Query: 61  DYSIPLFGDELELSILARFPQSEQWKLSCVSKRYLTLVRSGELYRIRKEIGYQESSVFML 120
           DYSIPLF DELELSILARFPQSEQWKLSCVSKRYLTLV+SGELYRIRKEIGYQESSVFML
Sbjct: 61  DYSIPLFADELELSILARFPQSEQWKLSCVSKRYLTLVKSGELYRIRKEIGYQESSVFML 120

Query: 121 ASGESSWMMFDRTFQSCRRLPVLPSDTCFLDADKESLCAGTDLFVTGRELTGGAIWRYQL 180
           ASGESSWMMFDRTFQSCRRLPVLPSDTCFLDADKESLCAGTDL VTGRELTGGAIWRYQL
Sbjct: 121 ASGESSWMMFDRTFQSCRRLPVLPSDTCFLDADKESLCAGTDLIVTGRELTGGAIWRYQL 180

Query: 181 VENKWIKGPSMISPRCLFASASCGSDAFVAGGIALEFSTEGAFGMGMEYGQTVLNTVEKY 240
           VENKW KGPSMISPRCLFASASCGSDAFVAGGIALEFSTEGAFGMGMEYGQTVLNTVEKY
Sbjct: 181 VENKWTKGPSMISPRCLFASASCGSDAFVAGGIALEFSTEGAFGMGMEYGQTVLNTVEKY 240

Query: 241 SPESSSWEPLPNMHRPRKKCSGCFMDNKFYVIGGRDKDGNHLTCGEVFDKEKNLWDLIEN 300
           SPESSSWEPLPNMHR RKKCSGCFMDNKFYVIGGRDKDGNHLTCGEVFDKEKNLWDLIEN
Sbjct: 241 SPESSSWEPLPNMHRSRKKCSGCFMDNKFYVIGGRDKDGNHLTCGEVFDKEKNLWDLIEN 300

Query: 301 MLEDTPISTSQSPPLVAVVNNELYSLEPASNELKVYLKERNEWKNLGPVPVHAVVNKGWG 360
           MLEDTPISTSQSPPLVAVVNNELYSLEPASNELKVYLKERNEWKNLGPVPVHAVVNKGWG
Sbjct: 301 MLEDTPISTSQSPPLVAVVNNELYSLEPASNELKVYLKERNEWKNLGPVPVHAVVNKGWG 360

Query: 361 VAFKSLGSELLVIGASVESSTNNSMSIYTCTPDPRADRLQWRRLDGGTNHLSPFILNCCV 420
           VAFKSLGSELLVIGASVESSTNNSMSIYTCTPDPRADRLQW+RLDGGTNHLSPFILNCCV
Sbjct: 361 VAFKSLGSELLVIGASVESSTNNSMSIYTCTPDPRADRLQWKRLDGGTNHLSPFILNCCV 420

Query: 421 MVA 423
           MVA
Sbjct: 421 MVA 423

BLAST of CSPI03G25600 vs. NCBI nr
Match: XP_038907049.1 (F-box/kelch-repeat protein At3g27150 [Benincasa hispida])

HSP 1 Score: 794.3 bits (2050), Expect = 5.2e-226
Identity = 385/424 (90.80%), Postives = 397/424 (93.63%), Query Frame = 0

Query: 1   MHKDKGLKAK-EGKGKLCRCN-GLRKNKDMMFRDLVPRSSPPRFIRDCGVGESSGLKPQD 60
           M+KDKGL+ + EGKGKLC C  GLR NK+M+F +LVPRSS PRF RD GVGESSGLKPQD
Sbjct: 1   MYKDKGLEGREEGKGKLCGCECGLRTNKNMLFGELVPRSSGPRFTRDSGVGESSGLKPQD 60

Query: 61  ADYSIPLFGDELELSILARFPQSEQWKLSCVSKRYLTLVRSGELYRIRKEIGYQESSVFM 120
           ADYSIPLFGDELELSILARFPQSEQWKLSCVSKRY  LV+SGELYRIRKEIGYQESSVFM
Sbjct: 61  ADYSIPLFGDELELSILARFPQSEQWKLSCVSKRYFALVKSGELYRIRKEIGYQESSVFM 120

Query: 121 LASGESSWMMFDRTFQSCRRLPVLPSDTCFLDADKESLCAGTDLFVTGRELTGGAIWRYQ 180
           LASGESSWMMFDRTFQSCRRLPVLPSDTCFLDADKESLCAGTDL VTGRELTGGAIWRYQ
Sbjct: 121 LASGESSWMMFDRTFQSCRRLPVLPSDTCFLDADKESLCAGTDLIVTGRELTGGAIWRYQ 180

Query: 181 LVENKWIKGPSMISPRCLFASASCGSDAFVAGGIALEFSTEGAFGMGMEYGQTVLNTVEK 240
           L ENKWIKGPSMISPRCLFASASCGSDA+VAGGIALEFSTEGAFGMG+EYGQTVLN VEK
Sbjct: 181 LAENKWIKGPSMISPRCLFASASCGSDAYVAGGIALEFSTEGAFGMGLEYGQTVLNAVEK 240

Query: 241 YSPESSSWEPLPNMHRPRKKCSGCFMDNKFYVIGGRDKDGNHLTCGEVFDKEKNLWDLIE 300
           Y+PES  WEPLPNMHR RKKCSGCFMDNKFYVIGGRDKDGNHLTCGEVFDKEKN W LIE
Sbjct: 241 YNPESLLWEPLPNMHRSRKKCSGCFMDNKFYVIGGRDKDGNHLTCGEVFDKEKNSWSLIE 300

Query: 301 NMLEDTPISTSQSPPLVAVVNNELYSLEPASNELKVYLKERNEWKNLGPVPVHAVVNKGW 360
           NMLED PISTSQSPPLVAV NNELYSLEP+SNELKVYLK RNEWKNLGPVPV AVVN GW
Sbjct: 301 NMLEDAPISTSQSPPLVAVANNELYSLEPSSNELKVYLKGRNEWKNLGPVPVLAVVNNGW 360

Query: 361 GVAFKSLGSELLVIGASVESSTNNSMSIYTCTPDPRADRLQWRRLDGGTNHLSPFILNCC 420
           GVAFKSLG ELLVIGASVESSTNNSMSIYTCTPDPRA+RLQWRRLD GTNHLSPFILNCC
Sbjct: 361 GVAFKSLGDELLVIGASVESSTNNSMSIYTCTPDPRANRLQWRRLDSGTNHLSPFILNCC 420

Query: 421 VMVA 423
           VMVA
Sbjct: 421 VMVA 424

BLAST of CSPI03G25600 vs. NCBI nr
Match: XP_023531711.1 (F-box/kelch-repeat protein At3g27150 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 743.4 bits (1918), Expect = 1.1e-210
Identity = 359/424 (84.67%), Postives = 386/424 (91.04%), Query Frame = 0

Query: 1   MHKDKGLKAKE-GKGKLCRC-NGLRKNKDMMFRDLVPRSSPPRFIRDCGVGESSGLKPQD 60
           M K KGL+ KE G   LCRC +GLRK K M++ DLV RSS PR   D GVGESSG KPQD
Sbjct: 1   MFKGKGLEGKEQGIESLCRCDSGLRKKKKMVYGDLVARSSTPRLAGDSGVGESSGSKPQD 60

Query: 61  ADYSIPLFGDELELSILARFPQSEQWKLSCVSKRYLTLVRSGELYRIRKEIGYQESSVFM 120
           ADYS+PLFGDELELSILARFPQ EQW+LSCVSKRYL LV+SGEL+ IRKEIGY+E SVFM
Sbjct: 61  ADYSVPLFGDELELSILARFPQCEQWRLSCVSKRYLDLVKSGELFAIRKEIGYKEPSVFM 120

Query: 121 LASGESSWMMFDRTFQSCRRLPVLPSDTCFLDADKESLCAGTDLFVTGRELTGGAIWRYQ 180
           LASGESSWMMFDRTFQSCRRLP+LPSD CFLDADKESLCAGTDL VTGRELTG  IWRYQ
Sbjct: 121 LASGESSWMMFDRTFQSCRRLPILPSDACFLDADKESLCAGTDLIVTGRELTGATIWRYQ 180

Query: 181 LVENKWIKGPSMISPRCLFASASCGSDAFVAGGIALEFSTEGAFGMGMEYGQTVLNTVEK 240
           LV NKWIKGPSMISPRCLFASA+CG++AFVAGGIALEFS+EGAFGMGMEYGQTVLNT EK
Sbjct: 181 LVGNKWIKGPSMISPRCLFASATCGANAFVAGGIALEFSSEGAFGMGMEYGQTVLNTAEK 240

Query: 241 YSPESSSWEPLPNMHRPRKKCSGCFMDNKFYVIGGRDKDGNHLTCGEVFDKEKNLWDLIE 300
           Y+PES SWEPLP+MHR RKKCSGCFMD+KFYVIGGRD+DGNHLTCGEVFD++KN WDLIE
Sbjct: 241 YNPESLSWEPLPDMHRARKKCSGCFMDDKFYVIGGRDRDGNHLTCGEVFDEKKNSWDLIE 300

Query: 301 NMLEDTPISTSQSPPLVAVVNNELYSLEPASNELKVYLKERNEWKNLGPVPVHAVVNKGW 360
           NMLED PISTSQSPPLVAVVNNELYSLEP+SN+LKVYLK+RNEWKNLGPVPV +VVNKGW
Sbjct: 301 NMLEDAPISTSQSPPLVAVVNNELYSLEPSSNQLKVYLKDRNEWKNLGPVPVLSVVNKGW 360

Query: 361 GVAFKSLGSELLVIGASVESSTNNSMSIYTCTPDPRADRLQWRRLDGGTNHLSPFILNCC 420
           GVAFKSLG ELLVIG+S ESSTNNSMSIYTC PDPRA++LQWRRL  GTNHLSPFILNCC
Sbjct: 361 GVAFKSLGDELLVIGSSSESSTNNSMSIYTCIPDPRAEQLQWRRLYCGTNHLSPFILNCC 420

Query: 421 VMVA 423
           VMVA
Sbjct: 421 VMVA 424

BLAST of CSPI03G25600 vs. NCBI nr
Match: KAG6581094.1 (SWI/SNF complex component SNF12-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 740.0 bits (1909), Expect = 1.2e-209
Identity = 363/421 (86.22%), Postives = 379/421 (90.02%), Query Frame = 0

Query: 1   MHKDKGLKAKEGKGKLCRCNGLRKNKDMMFRDL-VPRSSPPRFIRDCGVGESSGLKPQDA 60
           M+KDKGL+        C     +KN     RDL VPR+S PRF  DCGVGESSGLK QDA
Sbjct: 1   MYKDKGLEG-------CVSGSRKKNNR---RDLTVPRNSAPRFALDCGVGESSGLKSQDA 60

Query: 61  DYSIPLFGDELELSILARFPQSEQWKLSCVSKRYLTLVRSGELYRIRKEIGYQESSVFML 120
           DYSIPLFGDELELSILARFP+SEQWKLSCVSKRYL +V+SGELY+IRK IGY+E SVFML
Sbjct: 61  DYSIPLFGDELELSILARFPKSEQWKLSCVSKRYLAIVKSGELYKIRKAIGYKEPSVFML 120

Query: 121 ASGESSWMMFDRTFQSCRRLPVLPSDTCFLDADKESLCAGTDLFVTGRELTGGAIWRYQL 180
           ASGESSWMMFDRTFQSCRRLP+LPSD+CFLDADKESLCAGTDL VTGRELTGGAIWRYQL
Sbjct: 121 ASGESSWMMFDRTFQSCRRLPILPSDSCFLDADKESLCAGTDLIVTGRELTGGAIWRYQL 180

Query: 181 VENKWIKGPSMISPRCLFASASCGSDAFVAGGIALEFSTEGAFGMGMEYGQTVLNTVEKY 240
           VENKWI GPSMISPRCLFASAS GS+AFVAGGIALEFSTEGAFGMGMEYGQTVLNTVEKY
Sbjct: 181 VENKWINGPSMISPRCLFASASSGSNAFVAGGIALEFSTEGAFGMGMEYGQTVLNTVEKY 240

Query: 241 SPESSSWEPLPNMHRPRKKCSGCFMDNKFYVIGGRDKDGNHLTCGEVFDKEKNLWDLIEN 300
           +PES  WEPLPNMHR RKKCSGCFMDNKFYVIGGRD DGNHLTCGEVFD EKN WDLIEN
Sbjct: 241 NPESLLWEPLPNMHRSRKKCSGCFMDNKFYVIGGRDNDGNHLTCGEVFDTEKNSWDLIEN 300

Query: 301 MLEDTPISTSQSPPLVAVVNNELYSLEPASNELKVYLKERNEWKNLGPVPVHAVVNKGWG 360
           MLED PISTSQSPPLVAVVNNELYSLEP+SNELKVYLK RNEWKNLGPVPV AVVNKGWG
Sbjct: 301 MLEDAPISTSQSPPLVAVVNNELYSLEPSSNELKVYLKGRNEWKNLGPVPVLAVVNKGWG 360

Query: 361 VAFKSLGSELLVIGASVESSTNNSMSIYTCTPDPRADRLQWRRLDGGTNHLSPFILNCCV 420
           VAFKSLG ELLVIGASVESS N+SMSIYTC+PDPRADRLQW RLD GTNHLSPFILNCCV
Sbjct: 361 VAFKSLGDELLVIGASVESSANDSMSIYTCSPDPRADRLQWTRLDCGTNHLSPFILNCCV 411

BLAST of CSPI03G25600 vs. TAIR 10
Match: AT3G27150.1 (Galactose oxidase/kelch repeat superfamily protein )

HSP 1 Score: 390.2 bits (1001), Expect = 2.1e-108
Identity = 198/380 (52.11%), Postives = 265/380 (69.74%), Query Frame = 0

Query: 51  SSGLKPQDADY---SIPLFGDELELSILARFPQSEQWKLSCVSKRYLTLVRSGELYRIRK 110
           S+ LKP+DADY   ++P    ELE+ ILAR P+ E WKL  ++K +  L++S E++++R+
Sbjct: 56  STCLKPKDADYCLLNVPQLVYELEVEILARVPRFEYWKLKLLNKGFSRLLKSDEIFKVRR 115

Query: 111 EIGYQESSVFMLASGESSWMMFDRTFQSCRRLPVLPSDTCFLDADKESLCAGTDLFVTGR 170
           E G  E SVFML+SG++ W MFD+ F +C++LP LPSD CFL  DKESLCAGT L VTG+
Sbjct: 116 ERGVVEPSVFMLSSGDTCWTMFDKGFGNCQKLPELPSDICFLHGDKESLCAGTHLIVTGK 175

Query: 171 ELTGGAIWRYQLVENKWIKGPSMISPRCLFASASCGSDAFVAGGIALEFSTEGAFGMGME 230
           E    A+WRY+L  +KW KGP+MI+PR LFASA+CG+  FVAGG+ +E       G G  
Sbjct: 176 EEKSIALWRYELETSKWFKGPAMITPRILFASATCGTVVFVAGGLKIE-------GNGT- 235

Query: 231 YGQTVLNTVEKYSPESSSWEPLPNMHRPRKKCSGCFMDNKFYVIGGRDKDGNHLTCGEVF 290
               V+++VEKY  ++ +W  L  MH+ RK CSGC++  KFYV+GGRD++G +LTCGE +
Sbjct: 236 --MEVVDSVEKYDSKTKTWTLLRGMHKRRKFCSGCYLRGKFYVLGGRDENGQNLTCGESY 295

Query: 291 DKEKNLWDLIENMLEDTPISTSQSPPLVAVVNNELYSLEPASNELKVYLKERNEWKNLGP 350
           D++ N W+LI ++L+D   S+ QSPPL+AVV ++LYSLE ++NEL+VY    N WK LG 
Sbjct: 296 DEKTNTWELIPDILKDMSFSSVQSPPLIAVVGDDLYSLETSANELRVYDANANSWKKLGD 355

Query: 351 VPVHAVVNKGWGVAFKSLGSELLVIGASVESSTNNSMSIYTCTPDPR-ADRLQW---RRL 410
           VPV A  N GWGVAFKSLG +LLVIGAS   S   +MS+YT  P    A++L W   +R 
Sbjct: 356 VPVRAKSNGGWGVAFKSLGDKLLVIGASAGPSRAETMSVYTSRPSANPANKLYWEESKRC 415

Query: 411 DG-GTNHLSPFILNCCVMVA 423
            G   NH   FILNCCVM+A
Sbjct: 416 CGVRFNH---FILNCCVMIA 422

BLAST of CSPI03G25600 vs. TAIR 10
Match: AT5G40680.1 (Galactose oxidase/kelch repeat superfamily protein )

HSP 1 Score: 340.5 bits (872), Expect = 1.9e-93
Identity = 169/371 (45.55%), Postives = 235/371 (63.34%), Query Frame = 0

Query: 52  SGLKPQDADYSIPLFGDELELSILARFPQSEQWKLSCVSKRYLTLVRSGELYRIRKEIGY 111
           + L PQDA   +P    +LE+ I +R    + WKL+ ++K++  L++S E++++R+E G 
Sbjct: 55  TSLAPQDAHNGLPKLMFDLEVEIFSRLSCFQYWKLNLLNKQFSQLLQSREIFKVRRERGL 114

Query: 112 QESSVFMLASGESSWMMFDRTFQSCRRLPVLPSDTCFLDADKESLCAGTDLFVTGRELTG 171
            +  V M +SGE+ W+MFD+ F++ R+LP +PSD CF   DKE++ AGT L V GRE   
Sbjct: 115 VQPYVLMFSSGETCWVMFDKGFKNFRQLPEIPSDFCFFYGDKETITAGTHLIVIGREEKR 174

Query: 172 GAIWRYQLVENKWIKGPSMISPRCLFASASCGSDAFVAGGIALEFSTEGAFGMGMEYGQT 231
             +WRY+L  NKWI    MI+PR ++ASAS G+DAF AGGI  + S  G        G  
Sbjct: 175 IVVWRYELEVNKWINDTEMITPRVMYASASRGTDAFFAGGI--KTSENG--------GPD 234

Query: 232 VLNTVEKYSPESSSWEPLPNMHRPRKKCSGCFMDNKFYVIGGRDKDGNHLTCGEVFDKEK 291
           V+N  E+Y+ ++ +W+ +  MH+ RK  SGCF+  KFY +GGRD++  +LTCGE +D+  
Sbjct: 235 VVNVAERYNSDTKTWKAMKAMHKRRKFSSGCFLRGKFYALGGRDENDVYLTCGESYDELT 294

Query: 292 NLWDLIENMLEDTPISTSQSPPLVAVVNNELYSLEPASNELKVYLKERNEWKNLGPVPVH 351
           + W LI +ML+       QSPPL+AVV + LY LE   NEL VY    N WKNLG VPV 
Sbjct: 295 DSWKLIPDMLKGMTFMNPQSPPLIAVVKDNLYLLETWLNELWVYDINANVWKNLGVVPVK 354

Query: 352 AVVNKGWGVAFKSLGSELLVIGASVESSTNNSMSIYTCTPDPRADRLQWRRLDGGTNHLS 411
           A    GWGVAFKS+G  +LVIGASV  S +N MS+YTC P P+ +++ W         L 
Sbjct: 355 ANAALGWGVAFKSVGDRILVIGASVTKSWDNKMSVYTCCPFPKVEKITWEETSCDCVQLG 414

Query: 412 PFILNCCVMVA 423
            FI NCCVM+A
Sbjct: 415 HFIRNCCVMLA 415

BLAST of CSPI03G25600 vs. TAIR 10
Match: AT5G60570.1 (Galactose oxidase/kelch repeat superfamily protein )

HSP 1 Score: 256.5 bits (654), Expect = 3.7e-68
Identity = 151/371 (40.70%), Postives = 198/371 (53.37%), Query Frame = 0

Query: 54  LKPQDADYSIPLFGDELELSILARFPQSEQWKLSCVSKRYLTLVRSGELYRIRKEIGYQE 113
           L+   +D  +P   D++ L+ LA  P+S+   LSCV+K+Y  L+ SG L+ +RKE+G  E
Sbjct: 41  LRLGSSDSVLPGLIDDVALNCLAWVPRSDYPSLSCVNKKYNKLINSGHLFALRKELGIVE 100

Query: 114 SSVFMLASGESSWMMFDRTFQSCRRLPVLPSDTCFLDADKESLCAGTDLFVTGRELTGGA 173
             VFM+      W+MF    +    LP +P D CF  ADKESL    +L V GREL   A
Sbjct: 101 YLVFMVCD-PRGWLMFSPMKKKWMVLPKMPCDECFNHADKESLAVDDELLVFGRELFQFA 160

Query: 174 IWRYQLVENKWIKGPSMISPRCLFASASCGSDAFVAGGIALEFSTEGAFGMGMEYGQTVL 233
           IW+Y L    W+K   M  PRCLFAS S G  A VAG              G +    +L
Sbjct: 161 IWKYSLRSRCWVKCEGMHRPRCLFASGSLGGIAIVAG--------------GTDMNGNIL 220

Query: 234 NTVEKYSPESSSWEPLPNMHRPRKKCSGCFMDNKFYVIGGRDKDGNHLTCGEVFDKEKNL 293
            + E Y   S  WE LPNMH PR+ CSG FMD KFYVIGG       +T GE FD E   
Sbjct: 221 ASAELYDSSSGRWEMLPNMHSPRRLCSGFFMDGKFYVIGGMSSPNVSVTFGEEFDLETRK 280

Query: 294 WDLIENMLEDTPISTSQSPPLVAVVNNELYSLEPASNELKVYLKERNEWKNLGPVPVHAV 353
           W  IE M  +     +Q+PPLV VVNNEL++LE ++N +K Y K +N+W+ +G +P    
Sbjct: 281 WRKIEGMYPNVN-RAAQAPPLVVVVNNELFTLEYSTNMVKKYDKVKNKWEVMGRLPPMVD 340

Query: 354 VNKGWGVAFKSLGSELLVI----GASVESSTNNSMSIYTCTPDPRADRLQWRRLDGGTNH 413
            + GWG+AFK  G +LLV     G   E    NS    +   D     L W+ L G   +
Sbjct: 341 SSNGWGLAFKPCGDQLLVFCGQRGPHGEGIVVNSWCPKSGAKD---GNLDWKVL-GVKEN 391

Query: 414 LSPFILNCCVM 421
           +  F+ NC VM
Sbjct: 401 VGVFVYNCAVM 391

BLAST of CSPI03G25600 vs. TAIR 10
Match: AT1G14330.1 (Galactose oxidase/kelch repeat superfamily protein )

HSP 1 Score: 231.9 bits (590), Expect = 9.7e-61
Identity = 141/369 (38.21%), Postives = 196/369 (53.12%), Query Frame = 0

Query: 58  DADYSIPLFGDELELSILARFPQSEQWKLSCVSKRYLTLVRSGELYRIRKEIGYQESSVF 117
           D D  I   G +  +S L R  +S    ++ +++ + +LV++GE+YR+R++    E  V+
Sbjct: 90  DGDSLINDIGRDNSISCLIRCSRSGYGSIASLNRSFRSLVKTGEIYRLRRQNQIVEHWVY 149

Query: 118 MLASGESSWMMFDRTFQSCRRLPVLPSDTCFLDADKESLCAGTDLFVTGR-ELTGGAIWR 177
             +     W+ F+   +    LP +PS   F+ ADKESL  GTDL V G+ + +   I+R
Sbjct: 150 -FSCQLLEWVAFNPFERRWMNLPTMPSGVTFMCADKESLAVGTDLLVLGKDDYSSHVIYR 209

Query: 178 YQLVENKWIKGPSMISPRCLFASASCGSDAFVAGGIALEFSTEGAFGMGMEYGQTVLNTV 237
           Y L+ N W  G  M SPRCLF SAS G  A  AGG         +FG        + ++ 
Sbjct: 210 YSLLTNSWSSGMRMNSPRCLFGSASLGEIAIFAGGF-------DSFG-------KISDSA 269

Query: 238 EKYSPESSSWEPLPNMHRPRKKCSGCFMDNKFYVIGG-RDKDGNHLTCGEVFDKEKNLWD 297
           E Y+ E  +W  LP M++PRK CSG FMD KFYVIGG    D   LTCGE FD E   W 
Sbjct: 270 EMYNSELQTWTTLPKMNKPRKMCSGVFMDGKFYVIGGIGGNDSKVLTCGEEFDLETKKWT 329

Query: 298 LIENM----LEDTPISTSQSPPLVAVVNNELYSLEPASNELKVYLKERNEWKNLGPVPVH 357
            I  M      + P + +++PPLVAVVNNELY+ + A  E++ Y KE  +W  LG +P  
Sbjct: 330 EIPEMSPPRSREMP-AAAEAPPLVAVVNNELYAADHADMEVRKYDKESKKWFTLGRLPER 389

Query: 358 AVVNKGWGVAFKSLGSELLVIGASVESSTNNSMSIYTCTPDPRADRLQWRRLDGGTNHLS 417
           A    GWG+AF++ G  L+VIG    SS    + + +  P        W  L  G  H S
Sbjct: 390 ADSVNGWGLAFRACGERLIVIGGP-RSSGGGYIELNSWIPSSDRSPPLWTLL--GRKHSS 439

Query: 418 PFILNCCVM 421
            F+ NC VM
Sbjct: 450 NFVYNCAVM 439

BLAST of CSPI03G25600 vs. TAIR 10
Match: AT2G02870.1 (Galactose oxidase/kelch repeat superfamily protein )

HSP 1 Score: 231.9 bits (590), Expect = 9.7e-61
Identity = 138/378 (36.51%), Postives = 202/378 (53.44%), Query Frame = 0

Query: 52  SGLKPQDADYSIPLFGDELELSILARFPQSEQWKLSCVSKRYLTLVRSGELYRIRKEIGY 111
           +G    D+   I   G +  +  L R  +S+   ++ +++ + +LV+SGE+YR+R++ G+
Sbjct: 107 NGGDSSDSHSLINEIGRDNSIDCLIRCSRSDYGSIASLNRNFRSLVKSGEIYRLRRQNGF 166

Query: 112 QESSVFMLASGESSWMMFDRTFQSCRRLPVLPSDTCFLDADKESLCAGTDLFVTGR-ELT 171
            E  V+  +     W+ FD   +   +LP +PS   F+ ADKESL  GTDL V G+ + +
Sbjct: 167 VEHWVY-FSCQLLEWVAFDPVERRWMQLPTMPSSVTFMCADKESLAVGTDLLVLGKDDFS 226

Query: 172 GGAIWRYQLVENKWIKGPSMISPRCLFASASCGSDAFVAGGIALEFSTEGAFGMGMEYGQ 231
              I+RY L+ N W  G  M SPRCLF SAS G  A  AGG      ++G          
Sbjct: 227 SHVIYRYSLLTNSWSSGMKMNSPRCLFGSASLGEIAIFAGGC----DSQG---------- 286

Query: 232 TVLNTVEKYSPESSSWEPLPNMHRPRKKCSGCFMDNKFYVIGG-RDKDGNHLTCGEVFDK 291
            +L+  E Y+ E  +W  LP M++PRK CSG FMD KFYVIGG    D   LTCGE +D 
Sbjct: 287 KILDFAEMYNSELQTWITLPRMNKPRKMCSGVFMDGKFYVIGGIGGADSKGLTCGEEYDL 346

Query: 292 EKNLWDLIENM------LEDTPIS-TSQSPPLVAVVNNELYSLEPASNELKVYLKERNEW 351
           E   W  I ++       +   +S  +++PPLVAVVNN+LY+ + A  E++ Y KE  +W
Sbjct: 347 ETKKWTQIPDLSPPRSRADQADMSPAAEAPPLVAVVNNQLYAADHADMEVRKYDKENKKW 406

Query: 352 KNLGPVPVHAVVNKGWGVAFKSLGSELLVIGASVESSTNNSMSIYTCTPDPRADRLQWRR 411
             +G +P  A    GWG+AF++ G  L+VIG   + S    + + +  P       QW  
Sbjct: 407 LTVGRLPERAGSVNGWGLAFRACGERLIVIGGP-KCSGGGFIELNSWIPSDGGPP-QWTL 465

Query: 412 LDGGTNHLSPFILNCCVM 421
           LD    H   F+ NC VM
Sbjct: 467 LD--RKHSPTFVYNCAVM 465

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LI893.0e-10752.11F-box/kelch-repeat protein At3g27150 OS=Arabidopsis thaliana OX=3702 GN=At3g2715... [more]
Q9FKJ05.2e-6740.70F-box/kelch-repeat protein At5g60570 OS=Arabidopsis thaliana OX=3702 GN=At5g6057... [more]
Q8L7361.4e-5936.51F-box/kelch-repeat protein SKIP11 OS=Arabidopsis thaliana OX=3702 GN=SKIP11 PE=1... [more]
Q84M949.1e-5634.97F-box/kelch-repeat protein At1g26930 OS=Arabidopsis thaliana OX=3702 GN=At1g2693... [more]
Q9CA631.7e-5433.79F-box/kelch-repeat protein At1g74510 OS=Arabidopsis thaliana OX=3702 GN=At1g7451... [more]
Match NameE-valueIdentityDescription
A0A0A0L8A96.0e-252100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G586950 PE=4 SV=1[more]
A0A1S3BP523.5e-24497.64F-box/kelch-repeat protein At3g27150 OS=Cucumis melo OX=3656 GN=LOC103491782 PE=... [more]
A0A5A7THW53.5e-24497.64F-box/kelch-repeat protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff... [more]
A0A6J1F4C01.3e-20986.05F-box/kelch-repeat protein At3g27150-like OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A6J1J5B84.8e-20985.82F-box/kelch-repeat protein At3g27150-like OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
Match NameE-valueIdentityDescription
XP_004145670.11.2e-251100.00F-box/kelch-repeat protein At3g27150 [Cucumis sativus] >KGN58185.1 hypothetical ... [more]
XP_008450091.17.3e-24497.64PREDICTED: F-box/kelch-repeat protein At3g27150 [Cucumis melo] >XP_008450092.1 P... [more]
XP_038907049.15.2e-22690.80F-box/kelch-repeat protein At3g27150 [Benincasa hispida][more]
XP_023531711.11.1e-21084.67F-box/kelch-repeat protein At3g27150 [Cucurbita pepo subsp. pepo][more]
KAG6581094.11.2e-20986.22SWI/SNF complex component SNF12-like protein, partial [Cucurbita argyrosperma su... [more]
Match NameE-valueIdentityDescription
AT3G27150.12.1e-10852.11Galactose oxidase/kelch repeat superfamily protein [more]
AT5G40680.11.9e-9345.55Galactose oxidase/kelch repeat superfamily protein [more]
AT5G60570.13.7e-6840.70Galactose oxidase/kelch repeat superfamily protein [more]
AT1G14330.19.7e-6138.21Galactose oxidase/kelch repeat superfamily protein [more]
AT2G02870.19.7e-6136.51Galactose oxidase/kelch repeat superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006652Kelch repeat type 1SMARTSM00612kelc_smartcoord: 108..149
e-value: 3.7E-5
score: 33.1
coord: 150..203
e-value: 12.0
score: 2.3
coord: 44..87
e-value: 0.89
score: 12.1
IPR006652Kelch repeat type 1PFAMPF01344Kelch_1coord: 138..183
e-value: 2.4E-8
score: 33.4
coord: 76..135
e-value: 6.3E-8
score: 32.1
IPR015915Kelch-type beta propellerGENE3D2.120.10.80coord: 3..288
e-value: 2.8E-36
score: 127.0
IPR015915Kelch-type beta propellerSUPERFAMILY117281Kelch motifcoord: 7..272
NoneNo IPR availablePANTHERPTHR46122GALACTOSE OXIDASE/KELCH REPEAT PROTEIN-RELATEDcoord: 1..304
NoneNo IPR availablePANTHERPTHR46122:SF5KELCH REPEAT F-BOX PROTEINcoord: 1..304

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI03G25600.1CSPI03G25600.1mRNA
CSPI03G25600.2CSPI03G25600.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005829 cytosol
cellular_component GO:0005634 nucleus
molecular_function GO:0005515 protein binding