CSPI03G25430 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI03G25430
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Retrovirus-related Pol polyprotein from transposon TNT 1-94
Location	Chr3: 22774469 .. 22775631 (+)
RNA-Seq Expression	CSPI03G25430
Synteny	CSPI03G25430

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTATAGCAAGAGTAGAAATTGAGAAGTTTGATGGAAAGGGAGACTTTGCATTATGGAAAGCAAAGATCAAAGTCTTGCTTGGACAGCAAAAGTCTCATAAAGCCCTTTTTGATCCTTCAGAACTTCCAACAACCCTCACAGCAGCACAAAAAGAAGAAATGAAATTAATTGCCTATGGAACACTAATACTAAACCTTAGTGACAACATAATTAGGCAAGTTCTAGAAGAAGAAACAGCATATAAAGTTTGGATGAAACTAGAAAGCCTATATGCCTCTAAAGATCTCCCAAACAAAATGTACCTAAGGGAAAAAAATTTCACATATAAAATGGATCCTTCTAAAACCTTATCTGAAAACTTAGAAGAATTCAAGAAAATAGTTTCAGATTTTAAAACCCTTGAGTGACAAACTAAGTGATGAAAATGAGGCATTTGTCCTTTTAGATTCTTTACCCGAGGCATACAAAGAAGTAAAAAATGCTCTAAAATATGGAAGAGACTCAGTAAAAATAGATGTCATAATATCAGCTCTAAGAACTAGAGAATTAGAAATACATTCATCACACAAAGAAAATCATAGTGGTGATGGATTGTTTGTCAAAGGTAAACCCCAAAACAATCAAAGTAAAAACAGTAACAAATCTTTTCTAATGAAGACAAGAAAGGAAAACAGAAAAAGAAATGTAAATACTGTAAAAAGAAGGGACACCTCATATAAGAATGTTTTTTTCTTAAAAGAAAGAATCAAGAAGAAAAAAAAGATTCTAAAGGGAAACAACCAGAAGTGTCTATAGTAGAAAGCTCTTTTACCTACACAGATGCCTTGGCATCAACCTTAGACCAACCATGTCAACCCCTTAGGAAAACATGATTGGGTCCTAGACTCAGGATGCACCTACCATATGATACCTTTTAGAGCATGGTTCAATACCTATAGAGAGATCAGTGGAGAATTTGTGTTCATGGGGAATAATAATGAAACATTGCTGGAATTGGATCAGATGAAACTAAAAGATGGGACTGTAAAACTCCTTAGAAATGTTAGACATGTTCCTCGCCTCAAAAGAAATTTAATCTCCCTAGGAATGTTAGACTCTCTAGGGTGTGAATACAAAGGGAAATGTGGAGTTTTCGAAGTCTTTATGGGATCTAAGTTAG

mRNA sequence

ATGGCTATAGCAAGAGTAGAAATTGAGAAGTTTGATGGAAAGGGAGACTTTGCATTATGGAAAGCAAAGATCAAAGTCTTGCTTGGACAGCAAAAGTCTCATAAAGCCCTTTTTGATCCTTCAGAACTTCCAACAACCCTCACAGCAGCACAAAAAGAAGAAATGAAATTAATTGCCTATGGAACACTAATACTAAACCTTAGTGACAACATAATTAGGCAAGTTCTAGAAGAAGAAACAGCATATAAAGTTTGGATGAAACTAGAAAGCCTATATGCCTCTAAAGATCTCCCAAACAAAATAGCATGGTTCAATACCTATAGAGAGATCAGTGGAGAATTTGTGTTCATGGGGAATAATAATGAAACATTGCTGGAATTGGATCAGATGAAACTAAAAGATGGGACTGGTGTGAATACAAAGGGAAATGTGGAGTTTTCGAAGTCTTTATGGGATCTAAGTTAG

Coding sequence (CDS)

ATGGCTATAGCAAGAGTAGAAATTGAGAAGTTTGATGGAAAGGGAGACTTTGCATTATGGAAAGCAAAGATCAAAGTCTTGCTTGGACAGCAAAAGTCTCATAAAGCCCTTTTTGATCCTTCAGAACTTCCAACAACCCTCACAGCAGCACAAAAAGAAGAAATGAAATTAATTGCCTATGGAACACTAATACTAAACCTTAGTGACAACATAATTAGGCAAGTTCTAGAAGAAGAAACAGCATATAAAGTTTGGATGAAACTAGAAAGCCTATATGCCTCTAAAGATCTCCCAAACAAAATAGCATGGTTCAATACCTATAGAGAGATCAGTGGAGAATTTGTGTTCATGGGGAATAATAATGAAACATTGCTGGAATTGGATCAGATGAAACTAAAAGATGGGACTGGTGTGAATACAAAGGGAAATGTGGAGTTTTCGAAGTCTTTATGGGATCTAAGTTAG

Protein sequence

MAIARVEIEKFDGKGDFALWKAKIKVLLGQQKSHKALFDPSELPTTLTAAQKEEMKLIAYGTLILNLSDNIIRQVLEEETAYKVWMKLESLYASKDLPNKIAWFNTYREISGEFVFMGNNNETLLELDQMKLKDGTGVNTKGNVEFSKSLWDLS*

Homology

[Close]

BLAST of CSPI03G25430 vs. ExPASy Swiss-Prot
Match: P10978 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 3.5e-13
Identity = 37/101 (36.63%), Postives = 62/101 (61.39%), Query Frame = 0


Query: 1   MAIARVEIEKFDGKGDFALWKAKIKVLLGQQKSHKALFDPSELPTTLTAAQKEEMKLIAY 60
           M+  + E+ KF+G   F+ W+ +++ LL QQ  HK L   S+ P T+ A    ++   A 
Sbjct: 1   MSGVKYEVAKFNGDNGFSTWQRRMRDLLIQQGLHKVLDVDSKKPDTMKAEDWADLDERAA 60

Query: 61  GTLILNLSDNIIRQVLEEETAYKVWMKLESLYASKDLPNKI 102
             + L+LSD+++  +++E+TA  +W +LESLY SK L NK+
Sbjct: 61  SAIRLHLSDDVVNNIIDEDTARGIWTRLESLYMSKTLTNKL 101

[Close]

BLAST of CSPI03G25430 vs. ExPASy TrEMBL
Match: A0A5A7U6R2 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold333G00930 PE=4 SV=1)

HSP 1 Score: 128.3 bits (321), Expect = 2.9e-26
Identity = 66/132 (50.00%), Postives = 87/132 (65.91%), Query Frame = 0


Query: 1   MAIARVEIEKFDGKGDFALWKAKIKVLLGQQKSHKALFDPSELPTTLTAAQKEEMKLIAY 60
           M   + EIEKFDG GDF LW  +I  +LG QK+ KAL DP ELP TLT +++E ++ +AY
Sbjct: 1   MTTTKFEIEKFDGNGDFTLWTKRITAILGSQKALKALEDPKELPATLTKSERETLEEVAY 60

Query: 61  GTLILNLSDNIIRQVLEEETAYKVWMKLESLYASKDLPNKIAWFNTYREISGEFVFMGNN 120
            TLI+N++DN++RQV+EE TA+  W KL+SLY  KDLPNK+            F F  N 
Sbjct: 61  STLIMNITDNVLRQVIEETTAFATWEKLKSLYEKKDLPNKMFIKEKL------FSFKKNQ 120

Query: 121 NETLLE-LDQMK 132
           N+ L E LD+ K
Sbjct: 121 NKNLDENLDEFK 126

[Close]

BLAST of CSPI03G25430 vs. ExPASy TrEMBL
Match: A0A2P5FE65 (Uncharacterized protein (Fragment) OS=Trema orientale OX=63057 GN=TorRG33x02_080180 PE=4 SV=1)

HSP 1 Score: 120.2 bits (300), Expect = 7.8e-24
Identity = 59/101 (58.42%), Postives = 76/101 (75.25%), Query Frame = 0


Query: 1   MAIARVEIEKFDGKGDFALWKAKIKVLLGQQKSHKALFDPSELPTTLTAAQKEEMKLIAY 60
           M  +++EIEKFDGKGDF +WK K+K +L QQK  KAL D S LP T+  ++KEE+   AY
Sbjct: 1   MGTSKIEIEKFDGKGDFNMWKKKMKAVLVQQKCAKALGDVSGLPETMKPSEKEELLETAY 60

Query: 61  GTLILNLSDNIIRQVLEEETAYKVWMKLESLYASKDLPNKI 102
             LILNL+DN++RQV E++TA KVW KL+SLY +K L NKI
Sbjct: 61  SLLILNLADNVLRQVDEQDTAAKVWSKLDSLYLTKTLSNKI 101

[Close]

BLAST of CSPI03G25430 vs. ExPASy TrEMBL
Match: A0A5A7UZ07 (Retrotransposon protein, putative, Ty1-copia subclass OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold318G00070 PE=4 SV=1)

HSP 1 Score: 119.4 bits (298), Expect = 1.3e-23
Identity = 63/132 (47.73%), Postives = 87/132 (65.91%), Query Frame = 0


Query: 1   MAIARVEIEKFDGKGDFALWKAKIKVLLGQQKSHKALFDPSELPTTLTAAQKEEMKLIAY 60
           M   + EIEKFDG GDF L   +I  +LG QK+ KAL DP EL  TLT +++E ++ +AY
Sbjct: 1   MTTTKFEIEKFDGNGDFTLGTKRITAILGSQKALKALEDPKELLATLTKSERETLEEVAY 60

Query: 61  GTLILNLSDNIIRQVLEEETAYKVWMKLESLYASKDLPNKIAWFNTYREISGEFVFMGNN 120
           GTLI+N+++N++RQ++EE TA+  W KL+SLY  KDLPNK+   +        F F  N 
Sbjct: 61  GTLIMNITENVLRQIIEETTAFVTWEKLKSLYEKKDLPNKMFIRDKL------FSFKMNQ 120

Query: 121 NETLLE-LDQMK 132
           N+ L E LD+ K
Sbjct: 121 NKNLDETLDEFK 126

[Close]

BLAST of CSPI03G25430 vs. ExPASy TrEMBL
Match: A0A2P5CTT8 (Uncharacterized protein OS=Trema orientale OX=63057 GN=TorRG33x02_273130 PE=4 SV=1)

HSP 1 Score: 119.0 bits (297), Expect = 1.7e-23
Identity = 58/101 (57.43%), Postives = 75/101 (74.26%), Query Frame = 0


Query: 1   MAIARVEIEKFDGKGDFALWKAKIKVLLGQQKSHKALFDPSELPTTLTAAQKEEMKLIAY 60
           M  +++EIEKFDGKGDF +WK K+K +L QQK  K L D S LP T+  ++KEE+   AY
Sbjct: 1   MGTSKIEIEKFDGKGDFNMWKKKMKAVLVQQKCAKVLGDASGLPETMKPSEKEELLETAY 60

Query: 61  GTLILNLSDNIIRQVLEEETAYKVWMKLESLYASKDLPNKI 102
             LILNL+DN++RQV E++TA KVW KL+SLY +K L NKI
Sbjct: 61  SLLILNLADNVLRQVDEQDTAAKVWSKLDSLYLTKTLSNKI 101

[Close]

BLAST of CSPI03G25430 vs. ExPASy TrEMBL
Match: A0A5D3BQH4 (Transcription initiation factor TFIID subunit 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1112G00230 PE=4 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 6.6e-23
Identity = 52/92 (56.52%), Postives = 71/92 (77.17%), Query Frame = 0


Query: 1  MAIARVEIEKFDGKGDFALWKAKIKVLLGQQKSHKALFDPSELPTTLTAAQKEEMKLIAY 60
          MA+ R +IEKFDGKG F LWKAK+K ++GQ+K  KA+ DP +LP  +T  +KE M+  AY
Sbjct: 1  MAVERYDIEKFDGKGYFTLWKAKMKAIVGQRKKLKAIIDPEKLPKKMTQEEKETMENAAY 60

Query: 61 GTLILNLSDNIIRQVLEEETAYKVWMKLESLY 93
          G ++LNLSDN++R+V+ +ETAY +W KLE LY
Sbjct: 61 GAIVLNLSDNVLREVIGKETAYGMWQKLEELY 92

[Close]

BLAST of CSPI03G25430 vs. NCBI nr
Match: KAA0051442.1 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Cucumis melo var. makuwa] >TYK22211.1 Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Cucumis melo var. makuwa])

HSP 1 Score: 128.3 bits (321), Expect = 5.9e-26
Identity = 66/132 (50.00%), Postives = 87/132 (65.91%), Query Frame = 0


Query: 1   MAIARVEIEKFDGKGDFALWKAKIKVLLGQQKSHKALFDPSELPTTLTAAQKEEMKLIAY 60
           M   + EIEKFDG GDF LW  +I  +LG QK+ KAL DP ELP TLT +++E ++ +AY
Sbjct: 1   MTTTKFEIEKFDGNGDFTLWTKRITAILGSQKALKALEDPKELPATLTKSERETLEEVAY 60

Query: 61  GTLILNLSDNIIRQVLEEETAYKVWMKLESLYASKDLPNKIAWFNTYREISGEFVFMGNN 120
            TLI+N++DN++RQV+EE TA+  W KL+SLY  KDLPNK+            F F  N 
Sbjct: 61  STLIMNITDNVLRQVIEETTAFATWEKLKSLYEKKDLPNKMFIKEKL------FSFKKNQ 120

Query: 121 NETLLE-LDQMK 132
           N+ L E LD+ K
Sbjct: 121 NKNLDENLDEFK 126

[Close]

BLAST of CSPI03G25430 vs. NCBI nr
Match: XP_038880370.1 (uncharacterized protein LOC120072018 [Benincasa hispida])

HSP 1 Score: 122.1 bits (305), Expect = 4.2e-24
Identity = 61/111 (54.95%), Postives = 83/111 (74.77%), Query Frame = 0


Query: 1   MAIARVEIEKFDGKGDFALWKAKIKVLLGQQKSHKALFDPSELPTTLTAAQKEEMKLIAY 60
           MA  R EIEKFDGK DF L KAKIK +LGQQK+  A+ DP++ P TL+ A KE ++L AY
Sbjct: 1   MATIRYEIEKFDGKIDFELQKAKIKAVLGQQKTLLAITDPTKYPETLSEADKETIELNAY 60

Query: 61  GTLILNLSDNIIRQVLEEETAYKVWMKLESLYASKDLPNKIAW---FNTYR 109
           GT+ILN++D+I+RQ++++ TAY +W KL  +Y +KDLPNK  +   F TY+
Sbjct: 61  GTIILNVTDSILRQIVDQPTAYALWNKLNEIYLNKDLPNKAFFRERFFTYK 111

[Close]

BLAST of CSPI03G25430 vs. NCBI nr
Match: XP_038890043.1 (uncharacterized protein LOC120079747 [Benincasa hispida])

HSP 1 Score: 121.7 bits (304), Expect = 5.5e-24
Identity = 65/152 (42.76%), Postives = 92/152 (60.53%), Query Frame = 0


Query: 1   MAIARVEIEKFDGKGDFALWKAKIKVLLGQQKSHKALFDPSELPTTLTAAQKEEMKLIAY 60
           MA  R EIEKFD K DF LWK KIKV+LGQQK+  A+ DP++ P TLT A+KE +++ A 
Sbjct: 1   MATTRYEIEKFDSKTDFELWKVKIKVVLGQQKALLAITDPAKYPETLTEAEKETIEVNAC 60

Query: 61  GTLILNLSDNIIRQVLEEETAYKVWMKLESLYASKDLPNKIAW----------------- 120
           GT++LN++DN++RQV+ + TAY +W KL  +Y +KDL NK                    
Sbjct: 61  GTIVLNVTDNVLRQVINQPTAYALWNKLNEIYLNKDLSNKAFLRERFFTYKMDAAKSLTD 120

Query: 121 -FNTYREISGEFVFMGNN----NETLLELDQM 131
             N ++ +S EF  +G N    NE  + L+ +
Sbjct: 121 ILNEFKRLSSEFRSIGYNIGEENEAFILLNSL 152

[Close]

BLAST of CSPI03G25430 vs. NCBI nr
Match: PON96088.1 (hypothetical protein TorRG33x02_080180, partial [Trema orientale])

HSP 1 Score: 120.2 bits (300), Expect = 1.6e-23
Identity = 59/101 (58.42%), Postives = 76/101 (75.25%), Query Frame = 0


Query: 1   MAIARVEIEKFDGKGDFALWKAKIKVLLGQQKSHKALFDPSELPTTLTAAQKEEMKLIAY 60
           M  +++EIEKFDGKGDF +WK K+K +L QQK  KAL D S LP T+  ++KEE+   AY
Sbjct: 1   MGTSKIEIEKFDGKGDFNMWKKKMKAVLVQQKCAKALGDVSGLPETMKPSEKEELLETAY 60

Query: 61  GTLILNLSDNIIRQVLEEETAYKVWMKLESLYASKDLPNKI 102
             LILNL+DN++RQV E++TA KVW KL+SLY +K L NKI
Sbjct: 61  SLLILNLADNVLRQVDEQDTAAKVWSKLDSLYLTKTLSNKI 101

[Close]

BLAST of CSPI03G25430 vs. NCBI nr
Match: KAA0060284.1 (retrotransposon protein, putative, Ty1-copia subclass [Cucumis melo var. makuwa] >TYK22034.1 retrotransposon protein, putative, Ty1-copia subclass [Cucumis melo var. makuwa])

HSP 1 Score: 119.4 bits (298), Expect = 2.8e-23
Identity = 63/132 (47.73%), Postives = 87/132 (65.91%), Query Frame = 0


Query: 1   MAIARVEIEKFDGKGDFALWKAKIKVLLGQQKSHKALFDPSELPTTLTAAQKEEMKLIAY 60
           M   + EIEKFDG GDF L   +I  +LG QK+ KAL DP EL  TLT +++E ++ +AY
Sbjct: 1   MTTTKFEIEKFDGNGDFTLGTKRITAILGSQKALKALEDPKELLATLTKSERETLEEVAY 60

Query: 61  GTLILNLSDNIIRQVLEEETAYKVWMKLESLYASKDLPNKIAWFNTYREISGEFVFMGNN 120
           GTLI+N+++N++RQ++EE TA+  W KL+SLY  KDLPNK+   +        F F  N 
Sbjct: 61  GTLIMNITENVLRQIIEETTAFVTWEKLKSLYEKKDLPNKMFIRDKL------FSFKMNQ 120

Query: 121 NETLLE-LDQMK 132
           N+ L E LD+ K
Sbjct: 121 NKNLDETLDEFK 126

The following BLAST results are available for this feature:

BLAST of CSPI03G25430 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P10978	3.5e-13	36.63	Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum...	[more]

BLAST of CSPI03G25430 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7U6R2	2.9e-26	50.00	Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Cucumis melo var....	[more]
A0A2P5FE65	7.8e-24	58.42	Uncharacterized protein (Fragment) OS=Trema orientale OX=63057 GN=TorRG33x02_080...	[more]
A0A5A7UZ07	1.3e-23	47.73	Retrotransposon protein, putative, Ty1-copia subclass OS=Cucumis melo var. makuw...	[more]
A0A2P5CTT8	1.7e-23	57.43	Uncharacterized protein OS=Trema orientale OX=63057 GN=TorRG33x02_273130 PE=4 SV...	[more]
A0A5D3BQH4	6.6e-23	56.52	Transcription initiation factor TFIID subunit 2-like OS=Cucumis melo var. makuwa...	[more]

BLAST of CSPI03G25430 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0051442.1	5.9e-26	50.00	Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Cucumis melo var. m...	[more]
XP_038880370.1	4.2e-24	54.95	uncharacterized protein LOC120072018 [Benincasa hispida]	[more]
XP_038890043.1	5.5e-24	42.76	uncharacterized protein LOC120079747 [Benincasa hispida]	[more]
PON96088.1	1.6e-23	58.42	hypothetical protein TorRG33x02_080180, partial [Trema orientale]	[more]
KAA0060284.1	2.8e-23	47.73	retrotransposon protein, putative, Ty1-copia subclass [Cucumis melo var. makuwa]...	[more]

BLAST of CSPI03G25430 vs. NCBI nr


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PFAM	PF14223	Retrotran_gag_2	coord: 56..102 e-value: 1.1E-7 score: 31.7

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G25430.1	CSPI03G25430.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0044249	cellular biosynthetic process
biological_process	GO:0044260	cellular macromolecule metabolic process
biological_process	GO:0034641	cellular nitrogen compound metabolic process
biological_process	GO:1901576	organic substance biosynthetic process
biological_process	GO:0044238	primary metabolic process
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0016740	transferase activity