CSPI03G25420 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI03G25420
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	WRKY protein
Location	Chr3: 22770329 .. 22773423 (+)
RNA-Seq Expression	CSPI03G25420
Synteny	CSPI03G25420

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GGCATTTTCCGTGGTGTCTACGGCACAATTGGCAGCTATATATTTACTGTTCTTCCTCTTCCATTTCTCCAAATTTCTCCTCCCCCTTCTCTTCTTTTCTTTGCTCCCAATTTTTCACCTTTTTCTTTTCTCTTTCCAAGATCCCAAAAAACCAACAATTAAAGCACATATTATCATCAGATCACACAAATTCCTATTTTGTGATCATCATCCGGAAGAGCAAAATTCTTCATTATCAATATATATCTTTTTAATACTTCTTGATGGAGAATTATCAAATGTTTTTTCCTTGCTCCGATGGGGGCGGAGGACTGTCAGCCTATCATCATGCCGACATGTCGTCCGGCGGTGCTTCTGATATGTTCGGTAATTTTCAGGGTGGCGATATGGAGGCGGTAAGTGGGTTTTTGGGGATGAAAAGAGAGGTGGATGGCGGGGCGGTGGAAGCAGAGGGCGGAGGGAAAAAGAAGGGAGAGAAGAAGGTGAGGAAACCGAGATATGCTTTTCAGACAAGAAGCCAAGTTGACATTCTTGACGATGGGTATCGTTGGAGAAAATATGGTCAAAAAGCCGTCAAAAACAACAAGTTCCCGAGGTTAACTCTATTACCCCTTTTTTCTTTCATTTTTATTTCTTTTCCATTAAATCGACCTTCAGTTTGGCCTTTAACCATTTCTATCATATTTGATAGTAATAATGATTGAAATTCTTCTAAGACACAAAAGTGATTGATTCATGTTTACATAATTCATCTACATGATTCACTTTAGTACTGGTGATGAATTTTACTAAAAATTAAATTTCTTCATTTTGTTTAAATTGTTATTTCTTGTGGGGAATGAAAAGTTTTTTCTTTTTTTTTTTTTTTAAAAAAAAAACTATGCATGTCAAGTAAAAATAAAATGAATTAAATGATTAAGATTAAGTGAGATGACGAAAAATATAAAATTTTCATGAAAAGTTAAGATTATGTGATGTAAAATGAAGGGAATTTGAAGTATATAGGACGTTCGTGAATAGTGAAAAAATTAAGAGAATGTTAAGTAAACTGTTGGGTTAAATGGAAGAATTAAGATTAAGTGTTTATGGGTCTATTAATCAAAACAAATATATCTTTCATGCAAAGTTAAGGTTCTATTTAGTAAAAATGATGCAAATTTTCATCGAATAAATGATTGAGAATAAAATGAAAAAAGCCATTTTTCTTAATTATAATGATTTTGTTTAGCTTTTGAAATTTACGATTGGTTTTCATATTATTTTAATCAATTTTCAATAACTATAACAATTTTTTTAAAAAACAAAAACTAATTTTCTTTTGTCACTTCTCAAAATTTGGCTTGGGTTTGGTTAGTAGAAAATAAATTAATAACTAGTATTTAATAAGCTTGTTTGTTGAAAATTAAAAACAAATCAAATTATCAAATTGAATCAAAATTTGTATTCAGTGACTTTGGAAGTGAATGGCTCTAAGATTGTGTTTTATAAAATAATAAAAAAAAAACATCTCATTTACATGCAAAATCAAATATCATGAGTAGTGAAATAGAAGTTAGATTTGAGTACTTGAACTGAATATCAAAATTAAGTATTATGTATAAATATATATATATGTATGTATGCATGTACATATTTAATTAAAAACTAACACCTTGATCTTGTACGTAGCTTGTTAGTTTAAGCTTTGAAAATTGAAGTTAAAATAATGTATAATGAATTATTCTCTACCTAGCTTTACCTCTAAACTTATAGTATTTAATAGAAAGATTGAGTGCCCTCATTAAGCAATCATTGAAACTTTTGATTTCCTAACGAATATGATTATAGTTTGAGACTACCATAATCTTTAAACCCATATGTATGGTATTAAGAATAAGAGAGAAATGAGGGTGTTGACATTTTATAATAATCAATTCTATAACTTTCATTCAACTATAATAAATAAAACGCTTATATTCTTATATGTATATAATAGAGACAGTGATATTAATAATAAGATATATAGAGAATATAAAACACACATAGTTAAGATAGAGTTAATTACTATATGAATTCTTGGTTTTTTCTAACTATCTTGGATTGATTTAGTGTTAAAAACCATACATTGTCTCAATAACTAACTATGAGATTATGAATTACTTTGATGACCACTTTTTTATGAGTTACTTTGACACTTAAATATTGTAGGGTTAGACAAGTTGTCTCGTGAGATCATTAGTGAATATGTATGTAAGCTGACCCGAATACTCACATATAATATATATATATATACATATGTTGGTTTCTTCTCAATTATCATGAGTTTATGTATGACAACATACCCATGCCAACTTTTTTTTTGTTTTAATAGGCACATGGGTGCTTAACTTTAAGATGGTTTGTAATTGCTTTAAACACATTTTTTATATACCACTGATCATATGAGAGGGGTTTAATTAGCTTGGGTTCAAAATACTCTTTATGTGTGTCTCGATATTATGTTTATGTTATGACAAAAACGCATGATACATGCATGGATAATGAATCATAAATCTCATGTGTCGTTCTTGTCCAAATTAAAAAATATAGATTGTTTTCATACTTGATTGATTGATATATGAAGATGAAGAAGGGTGATGATTGATATATTTAATTATGTTGTGTGTATATATAGGAGCTATTATAGATGCACACATCAAGGATGCAATGTGAAGAAACAAGTGCAGAGGCTAACGAGAGATGAAGGGGTGGTGGTGACAACATATGAAGGAATGCACACACACTCCATTGATAAGCCAACAGATAACTTTGAACAAATATTGAGTAGAATGCAGATTTATAGTACTCCTTTTTGAGATTCTTCCATTTCATCATCAACCACAAAACAAGGAGATCTCAATATGATCACTATCAATATCAAATCACACATATTAATACTTGTTCATGTGAATTTGAACAAAGGCTGATCATGTTTTCTTTTTCTCTTTTTCTTTTCTTTCTTTCTTTTTTTTTCTTTTTTGGTATTTCCTTCATCACCTTCGGTGCTTTTCTTTTCTTTTCTTTCTTTTTACCTTTTTTCTTGTAGAAATTAATAAAGTTTGCACAAGCAGCTGATG

mRNA sequence

GGCATTTTCCGTGGTGTCTACGGCACAATTGGCAGCTATATATTTACTGTTCTTCCTCTTCCATTTCTCCAAATTTCTCCTCCCCCTTCTCTTCTTTTCTTTGCTCCCAATTTTTCACCTTTTTCTTTTCTCTTTCCAAGATCCCAAAAAACCAACAATTAAAGCACATATTATCATCAGATCACACAAATTCCTATTTTGTGATCATCATCCGGAAGAGCAAAATTCTTCATTATCAATATATATCTTTTTAATACTTCTTGATGGAGAATTATCAAATGTTTTTTCCTTGCTCCGATGGGGGCGGAGGACTGTCAGCCTATCATCATGCCGACATGTCGTCCGGCGGTGCTTCTGATATGTTCGGTAATTTTCAGGGTGGCGATATGGAGGCGGTAAGTGGGTTTTTGGGGATGAAAAGAGAGGTGGATGGCGGGGCGGTGGAAGCAGAGGGCGGAGGGAAAAAGAAGGGAGAGAAGAAGGTGAGGAAACCGAGATATGCTTTTCAGACAAGAAGCCAAGTTGACATTCTTGACGATGGGTATCGTTGGAGAAAATATGGTCAAAAAGCCGTCAAAAACAACAAGTTCCCGAGGAGCTATTATAGATGCACACATCAAGGATGCAATGTGAAGAAACAAGTGCAGAGGCTAACGAGAGATGAAGGGGTGGTGGTGACAACATATGAAGGAATGCACACACACTCCATTGATAAGCCAACAGATAACTTTGAACAAATATTGAGTAGAATGCAGATTTATAGTACTCCTTTTTGAGATTCTTCCATTTCATCATCAACCACAAAACAAGGAGATCTCAATATGATCACTATCAATATCAAATCACACATATTAATACTTGTTCATGTGAATTTGAACAAAGGCTGATCATGTTTTCTTTTTCTCTTTTTCTTTTCTTTCTTTCTTTTTTTTTCTTTTTTGGTATTTCCTTCATCACCTTCGGTGCTTTTCTTTTCTTTTCTTTCTTTTTACCTTTTTTCTTGTAGAAATTAATAAAGTTTGCACAAGCAGCTGATG

Coding sequence (CDS)

ATGGAGAATTATCAAATGTTTTTTCCTTGCTCCGATGGGGGCGGAGGACTGTCAGCCTATCATCATGCCGACATGTCGTCCGGCGGTGCTTCTGATATGTTCGGTAATTTTCAGGGTGGCGATATGGAGGCGGTAAGTGGGTTTTTGGGGATGAAAAGAGAGGTGGATGGCGGGGCGGTGGAAGCAGAGGGCGGAGGGAAAAAGAAGGGAGAGAAGAAGGTGAGGAAACCGAGATATGCTTTTCAGACAAGAAGCCAAGTTGACATTCTTGACGATGGGTATCGTTGGAGAAAATATGGTCAAAAAGCCGTCAAAAACAACAAGTTCCCGAGGAGCTATTATAGATGCACACATCAAGGATGCAATGTGAAGAAACAAGTGCAGAGGCTAACGAGAGATGAAGGGGTGGTGGTGACAACATATGAAGGAATGCACACACACTCCATTGATAAGCCAACAGATAACTTTGAACAAATATTGAGTAGAATGCAGATTTATAGTACTCCTTTTTGA

Protein sequence

MENYQMFFPCSDGGGGLSAYHHADMSSGGASDMFGNFQGGDMEAVSGFLGMKREVDGGAVEAEGGGKKKGEKKVRKPRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQGCNVKKQVQRLTRDEGVVVTTYEGMHTHSIDKPTDNFEQILSRMQIYSTPF*

Homology

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BLAST of CSPI03G25420 vs. ExPASy Swiss-Prot
Match: Q9FYA2 (Probable WRKY transcription factor 75 OS=Arabidopsis thaliana OX=3702 GN=WRKY75 PE=1 SV=1)

HSP 1 Score: 170.6 bits (431), Expect = 1.5e-41
Identity = 84/117 (71.79%), Postives = 98/117 (83.76%), Query Frame = 0


Query: 54  EVDGGAVEAEGGGK--KKGEKKVRKPRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPR 113
           E +   V +EG  K  +  +KK +K RYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPR
Sbjct: 28  EEESSKVRSEGCSKSVESSKKKGKKQRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPR 87

Query: 114 SYYRCTHQGCNVKKQVQRLTRDEGVVVTTYEGMHTHSIDKPTDNFEQILSRMQIYST 169
           SYYRCT+ GCNVKKQVQRLT D+ VVVTTYEG+H+H I+K T+NFE IL++MQIYS+
Sbjct: 88  SYYRCTYGGCNVKKQVQRLTVDQEVVVTTYEGVHSHPIEKSTENFEHILTQMQIYSS 144

[Close]

BLAST of CSPI03G25420 vs. ExPASy Swiss-Prot
Match: Q9S763 (Probable WRKY transcription factor 45 OS=Arabidopsis thaliana OX=3702 GN=WRKY45 PE=1 SV=1)

HSP 1 Score: 163.3 bits (412), Expect = 2.4e-39
Identity = 74/100 (74.00%), Postives = 87/100 (87.00%), Query Frame = 0


Query: 67  KKKGEKKVRKPRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQGCNVKKQ 126
           + K +KK R+ RYAFQTRSQVDILDDGYRWRKYGQKAVKNN FPRSYY+CT +GC VKKQ
Sbjct: 41  RSKKKKKEREARYAFQTRSQVDILDDGYRWRKYGQKAVKNNPFPRSYYKCTEEGCRVKKQ 100

Query: 127 VQRLTRDEGVVVTTYEGMHTHSIDKPTDNFEQILSRMQIY 167
           VQR   DEGVVVTTY+G+HTH++DKP+DNF  IL++M I+
Sbjct: 101 VQRQWGDEGVVVTTYQGVHTHAVDKPSDNFHHILTQMHIF 140

[Close]

BLAST of CSPI03G25420 vs. ExPASy Swiss-Prot
Match: Q9FFS3 (Probable WRKY transcription factor 24 OS=Arabidopsis thaliana OX=3702 GN=WRKY24 PE=1 SV=1)

HSP 1 Score: 131.7 bits (330), Expect = 7.8e-30
Identity = 63/104 (60.58%), Postives = 77/104 (74.04%), Query Frame = 0


Query: 66  GKKKGEKKVRK-PRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQGCNVK 125
           GK+  EK+ RK PR AF TRS  D+LDDGYRWRKYGQK+VK+N  PRSYYRCT+  CNVK
Sbjct: 72  GKELKEKRSRKVPRIAFHTRSDDDVLDDGYRWRKYGQKSVKHNAHPRSYYRCTYHTCNVK 131

Query: 126 KQVQRLTRDEGVVVTTYEGMHTHSIDKPTDNFEQILSRMQIYST 169
           KQVQRL +D  VVVTTYEG+H H  +K  +    +L ++Q  S+
Sbjct: 132 KQVQRLAKDPNVVVTTYEGVHNHPCEKLMETLNPLLRQLQFLSS 175

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BLAST of CSPI03G25420 vs. ExPASy Swiss-Prot
Match: Q8VWQ4 (Probable WRKY transcription factor 56 OS=Arabidopsis thaliana OX=3702 GN=WRKY56 PE=1 SV=1)

HSP 1 Score: 128.3 bits (321), Expect = 8.6e-29
Identity = 66/132 (50.00%), Postives = 83/132 (62.88%), Query Frame = 0


Query: 36  NFQGGDMEAVSGFLGMKREVDGGAVEAEGGGKKKGEKKVRKPRYAFQTRSQVDILDDGYR 95
           NF+GG  E      G+       +   +   K KG++ +   R AF TRS  D+LDDGYR
Sbjct: 63  NFRGGGGE----MGGLVSNNSNNSDHNKNCNKGKGKRTLAMQRIAFHTRSDDDVLDDGYR 122

Query: 96  WRKYGQKAVKNNKFPRSYYRCTHQGCNVKKQVQRLTRDEGVVVTTYEGMHTHSIDKPTDN 155
           WRKYGQK+VKNN  PRSYYRCT+  CNVKKQVQRL +D  VVVTTYEG+H H  +K  + 
Sbjct: 123 WRKYGQKSVKNNAHPRSYYRCTYHTCNVKKQVQRLAKDPNVVVTTYEGVHNHPCEKLMET 182

Query: 156 FEQILSRMQIYS 168
              +L ++Q  S
Sbjct: 183 LSPLLRQLQFLS 190

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BLAST of CSPI03G25420 vs. ExPASy Swiss-Prot
Match: Q8GY11 (Probable WRKY transcription factor 43 OS=Arabidopsis thaliana OX=3702 GN=WRKY43 PE=1 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 1.9e-28
Identity = 54/97 (55.67%), Postives = 74/97 (76.29%), Query Frame = 0


Query: 71  EKKVRKPRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQGCNVKKQVQRL 130
           +KK++ PR++F+T+S  DILDDGYRWRKYGQK+VKN+ +PRSYYRCT   CNVKKQVQRL
Sbjct: 10  DKKMKNPRFSFRTKSDADILDDGYRWRKYGQKSVKNSLYPRSYYRCTQHMCNVKKQVQRL 69

Query: 131 TRDEGVVVTTYEGMHTHSIDKPTDNFEQILSRMQIYS 168
           +++  +V TTYEG+H H  ++       +L ++Q  S
Sbjct: 70  SKETSIVETTYEGIHNHPCEELMQTLTPLLHQLQFLS 106

[Close]

BLAST of CSPI03G25420 vs. ExPASy TrEMBL
Match: A0A0A0LB34 (WRKY domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G567330 PE=4 SV=1)

HSP 1 Score: 350.9 bits (899), Expect = 3.0e-93
Identity = 170/170 (100.00%), Postives = 170/170 (100.00%), Query Frame = 0


Query: 1   MENYQMFFPCSDGGGGLSAYHHADMSSGGASDMFGNFQGGDMEAVSGFLGMKREVDGGAV 60
           MENYQMFFPCSDGGGGLSAYHHADMSSGGASDMFGNFQGGDMEAVSGFLGMKREVDGGAV
Sbjct: 1   MENYQMFFPCSDGGGGLSAYHHADMSSGGASDMFGNFQGGDMEAVSGFLGMKREVDGGAV 60

Query: 61  EAEGGGKKKGEKKVRKPRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQG 120
           EAEGGGKKKGEKKVRKPRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQG
Sbjct: 61  EAEGGGKKKGEKKVRKPRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQG 120

Query: 121 CNVKKQVQRLTRDEGVVVTTYEGMHTHSIDKPTDNFEQILSRMQIYSTPF 171
           CNVKKQVQRLTRDEGVVVTTYEGMHTHSIDKPTDNFEQILSRMQIYSTPF
Sbjct: 121 CNVKKQVQRLTRDEGVVVTTYEGMHTHSIDKPTDNFEQILSRMQIYSTPF 170

[Close]

BLAST of CSPI03G25420 vs. ExPASy TrEMBL
Match: A0A1S3BNI8 (probable WRKY transcription factor 75 OS=Cucumis melo OX=3656 GN=LOC103491801 PE=4 SV=1)

HSP 1 Score: 345.9 bits (886), Expect = 9.7e-92
Identity = 167/170 (98.24%), Postives = 168/170 (98.82%), Query Frame = 0


Query: 1   MENYQMFFPCSDGGGGLSAYHHADMSSGGASDMFGNFQGGDMEAVSGFLGMKREVDGGAV 60
           MENYQMFFPCSDGGGGLSAYHHADMSSGGASDMFGNFQGGDMEAVSGFLGMKREVDG  V
Sbjct: 1   MENYQMFFPCSDGGGGLSAYHHADMSSGGASDMFGNFQGGDMEAVSGFLGMKREVDGATV 60

Query: 61  EAEGGGKKKGEKKVRKPRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQG 120
           EAEGGG+KKGEKKVRKPRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQG
Sbjct: 61  EAEGGGRKKGEKKVRKPRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQG 120

Query: 121 CNVKKQVQRLTRDEGVVVTTYEGMHTHSIDKPTDNFEQILSRMQIYSTPF 171
           CNVKKQVQRLTRDEGVVVTTYEGMHTHSIDKPTDNFEQILSRMQIYSTPF
Sbjct: 121 CNVKKQVQRLTRDEGVVVTTYEGMHTHSIDKPTDNFEQILSRMQIYSTPF 170

[Close]

BLAST of CSPI03G25420 vs. ExPASy TrEMBL
Match: E7CEZ0 (WRKY protein OS=Cucumis sativus OX=3659 GN=WRKY56 PE=2 SV=1)

HSP 1 Score: 339.7 bits (870), Expect = 6.9e-90
Identity = 165/165 (100.00%), Postives = 165/165 (100.00%), Query Frame = 0


Query: 6   MFFPCSDGGGGLSAYHHADMSSGGASDMFGNFQGGDMEAVSGFLGMKREVDGGAVEAEGG 65
           MFFPCSDGGGGLSAYHHADMSSGGASDMFGNFQGGDMEAVSGFLGMKREVDGGAVEAEGG
Sbjct: 1   MFFPCSDGGGGLSAYHHADMSSGGASDMFGNFQGGDMEAVSGFLGMKREVDGGAVEAEGG 60

Query: 66  GKKKGEKKVRKPRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQGCNVKK 125
           GKKKGEKKVRKPRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQGCNVKK
Sbjct: 61  GKKKGEKKVRKPRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQGCNVKK 120

Query: 126 QVQRLTRDEGVVVTTYEGMHTHSIDKPTDNFEQILSRMQIYSTPF 171
           QVQRLTRDEGVVVTTYEGMHTHSIDKPTDNFEQILSRMQIYSTPF
Sbjct: 121 QVQRLTRDEGVVVTTYEGMHTHSIDKPTDNFEQILSRMQIYSTPF 165

[Close]

BLAST of CSPI03G25420 vs. ExPASy TrEMBL
Match: A0A6J1F6Z8 (probable WRKY transcription factor 75 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111441438 PE=4 SV=1)

HSP 1 Score: 288.1 bits (736), Expect = 2.4e-74
Identity = 149/174 (85.63%), Postives = 154/174 (88.51%), Query Frame = 0


Query: 1   MENYQMFFPCSDGGGGLSAYHHADMSSGGASDMFGNFQGGDMEAVSGFLGMKREVDGGAV 60
           MENYQMFFPCS+ GGGLSAY   D+SSG ASDMF NF GGD +A SG LG+K EV   AV
Sbjct: 1   MENYQMFFPCSN-GGGLSAYSQPDISSGTASDMFRNFHGGDPQA-SGVLGLKTEVVTAAV 60

Query: 61  EAE----GGGKKKGEKKVRKPRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRC 120
           EA+    G G KKGEKKVRKPRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRC
Sbjct: 61  EADGNCGGSGNKKGEKKVRKPRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRC 120

Query: 121 THQGCNVKKQVQRLTRDEGVVVTTYEGMHTHSIDKPTDNFEQILSRMQIYSTPF 171
           THQGCNVKKQVQRLTRDEGVVVTTYEGMHTHSIDKPTDNFEQILSRMQIYSTPF
Sbjct: 121 THQGCNVKKQVQRLTRDEGVVVTTYEGMHTHSIDKPTDNFEQILSRMQIYSTPF 172

[Close]

BLAST of CSPI03G25420 vs. ExPASy TrEMBL
Match: A0A6J1IZP7 (probable WRKY transcription factor 75 OS=Cucurbita maxima OX=3661 GN=LOC111482121 PE=4 SV=1)

HSP 1 Score: 284.6 bits (727), Expect = 2.6e-73
Identity = 147/174 (84.48%), Postives = 153/174 (87.93%), Query Frame = 0


Query: 1   MENYQMFFPCSDGGGGLSAYHHADMSSGGASDMFGNFQGGDMEAVSGFLGMKREVDGGAV 60
           MENYQMFFPCS+ GGGLSAY   D+SSG ASDMF NF GGD +A SG LG+K EV   AV
Sbjct: 1   MENYQMFFPCSN-GGGLSAYSQPDISSGTASDMFRNFHGGDPQA-SGVLGLKTEVVTAAV 60

Query: 61  EAE----GGGKKKGEKKVRKPRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRC 120
           EA+    G   KKGEKKVRKPRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRC
Sbjct: 61  EADRNCGGSANKKGEKKVRKPRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRC 120

Query: 121 THQGCNVKKQVQRLTRDEGVVVTTYEGMHTHSIDKPTDNFEQILSRMQIYSTPF 171
           THQGCNVKKQVQRLT+DEGVVVTTYEGMHTHSIDKPTDNFEQILSRMQIYSTPF
Sbjct: 121 THQGCNVKKQVQRLTKDEGVVVTTYEGMHTHSIDKPTDNFEQILSRMQIYSTPF 172

[Close]

BLAST of CSPI03G25420 vs. NCBI nr
Match: KGN58164.1 (hypothetical protein Csa_017507 [Cucumis sativus])

HSP 1 Score: 350.9 bits (899), Expect = 6.2e-93
Identity = 170/170 (100.00%), Postives = 170/170 (100.00%), Query Frame = 0


Query: 1   MENYQMFFPCSDGGGGLSAYHHADMSSGGASDMFGNFQGGDMEAVSGFLGMKREVDGGAV 60
           MENYQMFFPCSDGGGGLSAYHHADMSSGGASDMFGNFQGGDMEAVSGFLGMKREVDGGAV
Sbjct: 1   MENYQMFFPCSDGGGGLSAYHHADMSSGGASDMFGNFQGGDMEAVSGFLGMKREVDGGAV 60

Query: 61  EAEGGGKKKGEKKVRKPRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQG 120
           EAEGGGKKKGEKKVRKPRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQG
Sbjct: 61  EAEGGGKKKGEKKVRKPRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQG 120

Query: 121 CNVKKQVQRLTRDEGVVVTTYEGMHTHSIDKPTDNFEQILSRMQIYSTPF 171
           CNVKKQVQRLTRDEGVVVTTYEGMHTHSIDKPTDNFEQILSRMQIYSTPF
Sbjct: 121 CNVKKQVQRLTRDEGVVVTTYEGMHTHSIDKPTDNFEQILSRMQIYSTPF 170

[Close]

BLAST of CSPI03G25420 vs. NCBI nr
Match: XP_008450119.1 (PREDICTED: probable WRKY transcription factor 75 [Cucumis melo])

HSP 1 Score: 345.9 bits (886), Expect = 2.0e-91
Identity = 167/170 (98.24%), Postives = 168/170 (98.82%), Query Frame = 0


Query: 1   MENYQMFFPCSDGGGGLSAYHHADMSSGGASDMFGNFQGGDMEAVSGFLGMKREVDGGAV 60
           MENYQMFFPCSDGGGGLSAYHHADMSSGGASDMFGNFQGGDMEAVSGFLGMKREVDG  V
Sbjct: 1   MENYQMFFPCSDGGGGLSAYHHADMSSGGASDMFGNFQGGDMEAVSGFLGMKREVDGATV 60

Query: 61  EAEGGGKKKGEKKVRKPRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQG 120
           EAEGGG+KKGEKKVRKPRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQG
Sbjct: 61  EAEGGGRKKGEKKVRKPRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQG 120

Query: 121 CNVKKQVQRLTRDEGVVVTTYEGMHTHSIDKPTDNFEQILSRMQIYSTPF 171
           CNVKKQVQRLTRDEGVVVTTYEGMHTHSIDKPTDNFEQILSRMQIYSTPF
Sbjct: 121 CNVKKQVQRLTRDEGVVVTTYEGMHTHSIDKPTDNFEQILSRMQIYSTPF 170

[Close]

BLAST of CSPI03G25420 vs. NCBI nr
Match: NP_001295776.1 (probable WRKY transcription factor 75 [Cucumis sativus] >ADU52533.1 WRKY protein [Cucumis sativus])

HSP 1 Score: 339.7 bits (870), Expect = 1.4e-89
Identity = 165/165 (100.00%), Postives = 165/165 (100.00%), Query Frame = 0


Query: 6   MFFPCSDGGGGLSAYHHADMSSGGASDMFGNFQGGDMEAVSGFLGMKREVDGGAVEAEGG 65
           MFFPCSDGGGGLSAYHHADMSSGGASDMFGNFQGGDMEAVSGFLGMKREVDGGAVEAEGG
Sbjct: 1   MFFPCSDGGGGLSAYHHADMSSGGASDMFGNFQGGDMEAVSGFLGMKREVDGGAVEAEGG 60

Query: 66  GKKKGEKKVRKPRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQGCNVKK 125
           GKKKGEKKVRKPRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQGCNVKK
Sbjct: 61  GKKKGEKKVRKPRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQGCNVKK 120

Query: 126 QVQRLTRDEGVVVTTYEGMHTHSIDKPTDNFEQILSRMQIYSTPF 171
           QVQRLTRDEGVVVTTYEGMHTHSIDKPTDNFEQILSRMQIYSTPF
Sbjct: 121 QVQRLTRDEGVVVTTYEGMHTHSIDKPTDNFEQILSRMQIYSTPF 165

[Close]

BLAST of CSPI03G25420 vs. NCBI nr
Match: XP_038878726.1 (probable WRKY transcription factor 75 [Benincasa hispida])

HSP 1 Score: 315.8 bits (808), Expect = 2.2e-82
Identity = 159/174 (91.38%), Postives = 162/174 (93.10%), Query Frame = 0


Query: 1   MENYQMFFPCS---DGGGGLSAYHHADMSSGGASDMFGNFQGGDMEAVSGFLGMKREVD- 60
           MENYQMFFPCS   DGGGGLSAYHHADMSS G SDMFGNF GGDME VSGFLGMK EVD 
Sbjct: 1   MENYQMFFPCSGGGDGGGGLSAYHHADMSSSGGSDMFGNFHGGDME-VSGFLGMKTEVDA 60

Query: 61  GGAVEAEGGGKKKGEKKVRKPRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRC 120
             AVE +GGG+KKGEKKVRKPRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRC
Sbjct: 61  AAAVEVDGGGRKKGEKKVRKPRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRC 120

Query: 121 THQGCNVKKQVQRLTRDEGVVVTTYEGMHTHSIDKPTDNFEQILSRMQIYSTPF 171
           T+QGCNVKKQVQRLTRDEGVVVTTYEGMHTHSIDKPTDNFEQILSRMQIYSTPF
Sbjct: 121 TYQGCNVKKQVQRLTRDEGVVVTTYEGMHTHSIDKPTDNFEQILSRMQIYSTPF 173

[Close]

BLAST of CSPI03G25420 vs. NCBI nr
Match: XP_022934193.1 (probable WRKY transcription factor 75 isoform X1 [Cucurbita moschata] >KAG6581104.1 putative WRKY transcription factor 75, partial [Cucurbita argyrosperma subsp. sororia] >KAG7017835.1 putative WRKY transcription factor 75, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 288.1 bits (736), Expect = 4.9e-74
Identity = 149/174 (85.63%), Postives = 154/174 (88.51%), Query Frame = 0


Query: 1   MENYQMFFPCSDGGGGLSAYHHADMSSGGASDMFGNFQGGDMEAVSGFLGMKREVDGGAV 60
           MENYQMFFPCS+ GGGLSAY   D+SSG ASDMF NF GGD +A SG LG+K EV   AV
Sbjct: 1   MENYQMFFPCSN-GGGLSAYSQPDISSGTASDMFRNFHGGDPQA-SGVLGLKTEVVTAAV 60

Query: 61  EAE----GGGKKKGEKKVRKPRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRC 120
           EA+    G G KKGEKKVRKPRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRC
Sbjct: 61  EADGNCGGSGNKKGEKKVRKPRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRC 120

Query: 121 THQGCNVKKQVQRLTRDEGVVVTTYEGMHTHSIDKPTDNFEQILSRMQIYSTPF 171
           THQGCNVKKQVQRLTRDEGVVVTTYEGMHTHSIDKPTDNFEQILSRMQIYSTPF
Sbjct: 121 THQGCNVKKQVQRLTRDEGVVVTTYEGMHTHSIDKPTDNFEQILSRMQIYSTPF 172

[Close]

BLAST of CSPI03G25420 vs. TAIR 10
Match: AT5G13080.1 (WRKY DNA-binding protein 75 )

HSP 1 Score: 170.6 bits (431), Expect = 1.1e-42
Identity = 84/117 (71.79%), Postives = 98/117 (83.76%), Query Frame = 0


Query: 54  EVDGGAVEAEGGGK--KKGEKKVRKPRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPR 113
           E +   V +EG  K  +  +KK +K RYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPR
Sbjct: 28  EEESSKVRSEGCSKSVESSKKKGKKQRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPR 87

Query: 114 SYYRCTHQGCNVKKQVQRLTRDEGVVVTTYEGMHTHSIDKPTDNFEQILSRMQIYST 169
           SYYRCT+ GCNVKKQVQRLT D+ VVVTTYEG+H+H I+K T+NFE IL++MQIYS+
Sbjct: 88  SYYRCTYGGCNVKKQVQRLTVDQEVVVTTYEGVHSHPIEKSTENFEHILTQMQIYSS 144

[Close]

BLAST of CSPI03G25420 vs. TAIR 10
Match: AT3G01970.1 (WRKY DNA-binding protein 45 )

HSP 1 Score: 163.3 bits (412), Expect = 1.7e-40
Identity = 74/100 (74.00%), Postives = 87/100 (87.00%), Query Frame = 0


Query: 67  KKKGEKKVRKPRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQGCNVKKQ 126
           + K +KK R+ RYAFQTRSQVDILDDGYRWRKYGQKAVKNN FPRSYY+CT +GC VKKQ
Sbjct: 41  RSKKKKKEREARYAFQTRSQVDILDDGYRWRKYGQKAVKNNPFPRSYYKCTEEGCRVKKQ 100

Query: 127 VQRLTRDEGVVVTTYEGMHTHSIDKPTDNFEQILSRMQIY 167
           VQR   DEGVVVTTY+G+HTH++DKP+DNF  IL++M I+
Sbjct: 101 VQRQWGDEGVVVTTYQGVHTHAVDKPSDNFHHILTQMHIF 140

[Close]

BLAST of CSPI03G25420 vs. TAIR 10
Match: AT5G41570.1 (WRKY DNA-binding protein 24 )

HSP 1 Score: 131.7 bits (330), Expect = 5.5e-31
Identity = 63/104 (60.58%), Postives = 77/104 (74.04%), Query Frame = 0


Query: 66  GKKKGEKKVRK-PRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQGCNVK 125
           GK+  EK+ RK PR AF TRS  D+LDDGYRWRKYGQK+VK+N  PRSYYRCT+  CNVK
Sbjct: 72  GKELKEKRSRKVPRIAFHTRSDDDVLDDGYRWRKYGQKSVKHNAHPRSYYRCTYHTCNVK 131

Query: 126 KQVQRLTRDEGVVVTTYEGMHTHSIDKPTDNFEQILSRMQIYST 169
           KQVQRL +D  VVVTTYEG+H H  +K  +    +L ++Q  S+
Sbjct: 132 KQVQRLAKDPNVVVTTYEGVHNHPCEKLMETLNPLLRQLQFLSS 175

[Close]

BLAST of CSPI03G25420 vs. TAIR 10
Match: AT1G64000.1 (WRKY DNA-binding protein 56 )

HSP 1 Score: 128.3 bits (321), Expect = 6.1e-30
Identity = 66/132 (50.00%), Postives = 83/132 (62.88%), Query Frame = 0


Query: 36  NFQGGDMEAVSGFLGMKREVDGGAVEAEGGGKKKGEKKVRKPRYAFQTRSQVDILDDGYR 95
           NF+GG  E      G+       +   +   K KG++ +   R AF TRS  D+LDDGYR
Sbjct: 63  NFRGGGGE----MGGLVSNNSNNSDHNKNCNKGKGKRTLAMQRIAFHTRSDDDVLDDGYR 122

Query: 96  WRKYGQKAVKNNKFPRSYYRCTHQGCNVKKQVQRLTRDEGVVVTTYEGMHTHSIDKPTDN 155
           WRKYGQK+VKNN  PRSYYRCT+  CNVKKQVQRL +D  VVVTTYEG+H H  +K  + 
Sbjct: 123 WRKYGQKSVKNNAHPRSYYRCTYHTCNVKKQVQRLAKDPNVVVTTYEGVHNHPCEKLMET 182

Query: 156 FEQILSRMQIYS 168
              +L ++Q  S
Sbjct: 183 LSPLLRQLQFLS 190

[Close]

BLAST of CSPI03G25420 vs. TAIR 10
Match: AT2G46130.1 (WRKY DNA-binding protein 43 )

HSP 1 Score: 127.1 bits (318), Expect = 1.4e-29
Identity = 54/97 (55.67%), Postives = 74/97 (76.29%), Query Frame = 0


Query: 71  EKKVRKPRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQGCNVKKQVQRL 130
           +KK++ PR++F+T+S  DILDDGYRWRKYGQK+VKN+ +PRSYYRCT   CNVKKQVQRL
Sbjct: 10  DKKMKNPRFSFRTKSDADILDDGYRWRKYGQKSVKNSLYPRSYYRCTQHMCNVKKQVQRL 69

Query: 131 TRDEGVVVTTYEGMHTHSIDKPTDNFEQILSRMQIYS 168
           +++  +V TTYEG+H H  ++       +L ++Q  S
Sbjct: 70  SKETSIVETTYEGIHNHPCEELMQTLTPLLHQLQFLS 106

The following BLAST results are available for this feature:

BLAST of CSPI03G25420 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FYA2	1.5e-41	71.79	Probable WRKY transcription factor 75 OS=Arabidopsis thaliana OX=3702 GN=WRKY75 ...	[more]
Q9S763	2.4e-39	74.00	Probable WRKY transcription factor 45 OS=Arabidopsis thaliana OX=3702 GN=WRKY45 ...	[more]
Q9FFS3	7.8e-30	60.58	Probable WRKY transcription factor 24 OS=Arabidopsis thaliana OX=3702 GN=WRKY24 ...	[more]
Q8VWQ4	8.6e-29	50.00	Probable WRKY transcription factor 56 OS=Arabidopsis thaliana OX=3702 GN=WRKY56 ...	[more]
Q8GY11	1.9e-28	55.67	Probable WRKY transcription factor 43 OS=Arabidopsis thaliana OX=3702 GN=WRKY43 ...	[more]

BLAST of CSPI03G25420 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LB34	3.0e-93	100.00	WRKY domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G567330 PE=4 S...	[more]
A0A1S3BNI8	9.7e-92	98.24	probable WRKY transcription factor 75 OS=Cucumis melo OX=3656 GN=LOC103491801 PE...	[more]
E7CEZ0	6.9e-90	100.00	WRKY protein OS=Cucumis sativus OX=3659 GN=WRKY56 PE=2 SV=1	[more]
A0A6J1F6Z8	2.4e-74	85.63	probable WRKY transcription factor 75 isoform X1 OS=Cucurbita moschata OX=3662 G...	[more]
A0A6J1IZP7	2.6e-73	84.48	probable WRKY transcription factor 75 OS=Cucurbita maxima OX=3661 GN=LOC11148212...	[more]

BLAST of CSPI03G25420 vs. NCBI nr


Match Name	E-value	Identity	Description
KGN58164.1	6.2e-93	100.00	hypothetical protein Csa_017507 [Cucumis sativus]	[more]
XP_008450119.1	2.0e-91	98.24	PREDICTED: probable WRKY transcription factor 75 [Cucumis melo]	[more]
NP_001295776.1	1.4e-89	100.00	probable WRKY transcription factor 75 [Cucumis sativus] >ADU52533.1 WRKY protein...	[more]
XP_038878726.1	2.2e-82	91.38	probable WRKY transcription factor 75 [Benincasa hispida]	[more]
XP_022934193.1	4.9e-74	85.63	probable WRKY transcription factor 75 isoform X1 [Cucurbita moschata] >KAG658110...	[more]

BLAST of CSPI03G25420 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G13080.1	1.1e-42	71.79	WRKY DNA-binding protein 75	[more]
AT3G01970.1	1.7e-40	74.00	WRKY DNA-binding protein 45	[more]
AT5G41570.1	5.5e-31	60.58	WRKY DNA-binding protein 24	[more]
AT1G64000.1	6.1e-30	50.00	WRKY DNA-binding protein 56	[more]
AT2G46130.1	1.4e-29	55.67	WRKY DNA-binding protein 43	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003657	WRKY domain	SMART	SM00774	WRKY_cls	coord: 90..149 e-value: 5.4E-39 score: 145.5
IPR003657	WRKY domain	PFAM	PF03106	WRKY	coord: 91..147 e-value: 3.2E-26 score: 91.3
IPR003657	WRKY domain	PROSITE	PS50811	WRKY	coord: 85..150 score: 29.539446
IPR036576	WRKY domain superfamily	GENE3D	2.20.25.80	WRKY domain	coord: 67..150 e-value: 2.4E-33 score: 115.8
IPR036576	WRKY domain superfamily	SUPERFAMILY	118290	WRKY DNA-binding domain	coord: 82..149
None	No IPR available	PANTHER	PTHR31221:SF222	WRKY TRANSCRIPTION FACTOR 75-RELATED	coord: 54..165
IPR044810	WRKY transcription factor, plant	PANTHER	PTHR31221	WRKY TRANSCRIPTION FACTOR PROTEIN 1-RELATED	coord: 54..165

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G25420.1	CSPI03G25420.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006355	regulation of transcription, DNA-templated
cellular_component	GO:0005634	nucleus
molecular_function	GO:0003700	DNA-binding transcription factor activity
molecular_function	GO:0043565	sequence-specific DNA binding