CSPI03G25410 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI03G25410
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	NAD(P)-binding Rossmann-fold superfamily protein
Location	Chr3: 22732113 .. 22736700 (+)
RNA-Seq Expression	CSPI03G25410
Synteny	CSPI03G25410

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GGATGTATAAGTTTTATAGAAATGGGTAAAAGTTTTTGCCTTTGGGTTGGTAGTAAAATTTTACTTGTATTCCATTGTTTCGGGTCCTCTTCTCTAACTTTCTTATGAATTAACATGTTGAATAATCAACATTCAATTCATTTTGTACTCGTCAACTGTTGACTGCTATGCATATTTGAGGTGATTTTTTTCTATACCAATTTCCTGGAAATTCCATCTCTGTCTTTGTTTTCTCTTCATAATCCTTCAATGATTCTTCTTTTTTCCCCAGGAATTGAAGACCCATTTGCTTTCCAAGATGGAGGGATCGACCAAGAACGTCTTACTTTCTTCCGGTGGGGATGAGATCTCCAAGAATCTGGCTCTTCATCTTGCCAGAAGGGGTTGCAGGTTCAGCATCCAACTTATAACTTGATGGTTCTGTTTGCATATTTATTGAGGAAATTGAGAGATATGGAGGCATTCATACCTTTTGTTATGGGGTTTCTCTCTTCTGTTTTGTATGCCTCTTTAATGCATTTTTTATTCACAGTTGTTAACCTTGTATCCATCTTTTTCATGGCTATGTCCAGATTAGTTTTGATTGGAAATGAGTGTGTTCTTCAGAGCATGAGTAAGATGATAGCGGAATCGTTAAAGGGTGTACTGCCAATTGAGGTTGTTGGGGTGGACATGGAAGAAGAGAGAGAGGCGGCTTTTGATGAGGCTGTGAACAGGGCATGCGGTATTTTGGGAACTTTGGATGCTTTCGTCCATGCTTATTCTTATGAAGGTACAACCCTTCTCTCTCTTTCCTCCATACTTTCTTTTCATTACAATGATGTAGTAAGGTTGCTATGTTGTTTTTGGTTTGACTGTTTAGGGTTCTTTATATTAGTTAGGTGTTGATTCTTTGTTTGGGCATGTTTTGATATTGTGGGGAAGATTGAATACAGATTTTCGGTGTATATTGGAAAGAGGTTAGGCTACACCATCCTGACTTTTCTGAAGTATGTGCTCATAGCTTTTGGTGCAGTCAGTGACTGTTTACAGATATCGGTTTATTTCACTGAGCTTAGAACTGAAGCTAGAAGGGTCTTTCTGAGAGTTAATGGTTTTAGTATGGATTCTAATTTCAAATGAGAATTTACGTTCAAAAGGCTTCTGAGGCATATGTTTTCAGGTTGCCTTCGGGCCTCGGGGTAGCTTCAAACCTTATCCCTCCCTTGTGTTGTAAATACAACAGGCACAAAGTCTTTTGTTGCGCCTTTGTGAGAATGCAATGGGGGCTTGAGGCTTACAATATGTATGCCTATGTAAGCCTATGTAATAGTTAGAAGAATGATGTTAAATTGTTCTCTTGAAAAAAGTTTTCATATTTACCATTGCGTATGTGTGTATATGTGGTTTTTTTCTTTCTTTAAAATTCAAATTAAGTCTTATGCCTCATGCTTCCCTTAAAGAAAATAAAATTAAAAATAATGCAATGCAGCTTTTAGCCGTTTAAAGCATTGGCAAGAACAGAAAGGAGTTGTTCTCTTAAGCCTTAATTGTGTGTGTATATGTACTTTTTTTATCTGTATAGAATTTCTTCCATTTTTCCTATGGTATATTGATTTTTAATTGTTTAGTATCACTCAATATTTGACAAAAGTAGTTGACAAATGATCTTTAGAAGTCCACTCTGTTCTACTCATAAGAAAAGTCTGCTGCAAGTAAATGGTGTATCAACAAGCGGAACATCGTGAAAATCTTTTTTCTTCAGGACAAAGTTTTGAAGAGCCCTCCCTTTCTTAATGAAGAACCAAGGAATTACCGTCCATTTTATCTTCCATAAATTTCTTCGAAATGTCTTTTGTGGATGCTCCATGCAACAATTAAGAACCCTTAAAATGTAATTATTGCTATCTTGGTTTGAAATGAAATAAACTTCATTTACAAAAAAATAGGATTTTATCAGTACACTTGTCATCCTCTCTGTCGAGCCATTGAGACAATATGATGCCTTTTAATAGAACCAAGCCAGAATTTAAGGAAAAATAATTAGCTTTTAAAAACTAGTGCCATGTACAATTGTATGCATATTAATTGGTTTACATTTTCTTTAAGATATAATACTGAACTCTGCTATTGGATGGTGAAAATTAGGATGTAGAAATTCTTTGAAAGCTATTCTACTGTAAACCGCGTTAACTGCACTGTGTTTGAAGCAGGTCCCATACAAGATGCTTTACAACTATCTGAAGAGGAATTCAAAAAGATTGTTAAGATAAATTTGATGGCCTCATGGTTTCTGATGAAGGCTGTTTGTCGAAGAATGCGAGACCAGAAATCAGGAGGTTCAGTAATTTTTTTAACCACCTTAATTGGTGCTGAGAGGGGATTGTATCCAGGAGGAGCTGCATATGGATCATGCTCAGCAGGCTTACAGCAGTTAGCTCGAGTAAGTAGTTGTCAACTTGTCATTGGTTGCTGCCTCTGGTGATTCTTCTTTGTTTCATTGCTGCTATTATTTTTTGCTTGCTGGGCTCCTTTTTCACGAGTGTATTTCTGCATCATTAGTTGTTAGAATAAGTTATTCATACGTCAGACTTCATCTTCCCCACTTGGCATCTTAATATATTCTTTTATATACCTTGAAGTTGTTGAGTCTATGCGACATATATTCATTTCAATGGTGTTTAACTACAATCTGGCTGATATTCTCTCTTCATACCCATAGTTGTCAATTGTCGATTTTTCTGTTCACCATCACCTCACTCCCTTGAATAACAAAGCTACTTGGTATGTCAAGACGAGATGAGGGTTTAAAGAAATGGTTGAGCTATGAGTAGGGGATAGATTTCAATCAAAAGCCCAAAAAGAAGATTTAAAATGCACCAAGATTTAACATCTTCCATGAGCTATCTACCTCTATAGATGTTCTTCTTTTCCTTAGATTTTTCGGCGTCTCAGATTGTCTCACAATGCCTTCTAACACAATAAAAAACCTTGCTACATACATTTCATAAATCATCTGAGAAACTTTTTGAAAGTCTTATAGAACCGAACTATATTGAACCTGAGAATTCTTTGATTCCGGAAAGATGGCGATATATTCTTGTGTATAGTTTGAATTGAAATGGAAAGATTTATGTAGTCAAGTCCAGCCCTTCAAATGAGCTTTGTTGCTTTTATTTTTCTTAAATGCTGTTAATTAGAAATCAGAACAAAAGATCTTTAATCTAGTGTATCTTGAACTAGTTATGGTACAAGGTTTATAGTGCTGTTTTTTTGTTGGAAACAAACTTGACCAACCCTCTTTTGTAAATTAGTCTGATTGTAGAAAAGATTGAAATCACAGTTGAAGTTGCAATCCTAAAAATTATACAAATCACCCAAATTTCAGTTTTTTGCAAGGGTTGGACTTTCCAGTAGAGATTTTTCAAAATTCCACTGGGGGAATAACCTGATCTCTGTTTGTCCTTGAATTTCCCTGACATGATAACATGTATGATGGATTTTTCTAGACTTCAGCTCTGGATGTTGGAAAGTATAAGATCAGGGTGAACGCAATCGCTCGTGGATTGCACCTGGATGATGGATATCCTGTATCAGTGGGCAAGGAACGTGCAGAGAAGCAAGTCAAGGATGCAGCCCCGCTAGAAAGATGGCTCGATGTCAAGGATGATTTAGCTTCAACTGTCATTTATCTAATCAGCGATGGGTCACGGTACATGACTGGAACAACCATCTTTGTTGATGGGGCACAGTCTCTAGTGAGGCCACGCATGCGCTCATATATGTAGTCCTGGTTATATTTGGTGGATGGTCAGCCTTTGGTGAGAACACCTATTTGTTTAAAACCATCTTGCATATGGATGAACATCTTGTTTCTGTGAGTTATTGTTTAAGTCCTGAATCTTTTGTTTCTGCTGGGTAGGGGGTGGATGTAGGCCATTGTTGATTCACTCAACCACTGTAACTGGAGCTGTTGTTCAACTAATGAATAATGTCCTCATAGGCTCTGTATTTGCTCGACCATTCTCGTGAATAAAGTCGAGGTATGCCCGGGGGTGCTTCCTTTAAATCAATTATTCTCTTCTTGATTCACTCAACCCATTGAGACTGTTTCCCCCTAGTACTCATGAATAAATTTGAAGTTCAGTGAAAGTATGTTTGAGTTGATCATTTCTTTAAAAAGCCTTTTTTTCTTCATTCATAGTTGAAGTTATCTCTTTTCCAAAATTCCAGTTTATAAATAGTGATTTCAAACTGTAATTTCAATTTTACAAGGGACTGCGAATGCAAAGCCCTTGGTCAGGGGCAAAGCAAAGGAAACAATGCAACATGAAATTTGGTTTGTGTATATATATACACTACAATAGGTGTAGGGTTCAAATTATAGACGTTTTGGTTGTTGATATGTCCAGTTGAGTTATGTGTTAGTAGCATCTAACACATCACATGCTCTGTGTGAGACAACTTGATGAATTTGAGTGGTGTTTGAAAGGCGAACTAGTCCAATAATTTCATGTGCCAAAATGTGGTTAAGCTTGTAGCAAAAAGCTTCTTGATTAATTAAATCAATTGTAAACAAACAAAAACAATTGGCATTGACATGAATTTG

mRNA sequence

GGATGTATAAGTTTTATAGAAATGGGTAAAAGTTTTTGCCTTTGGGTTGGTAGTAAAATTTTACTTGTATTCCATTGTTTCGGGTCCTCTTCTCTAACTTTCTTATGAATTAACATGTTGAATAATCAACATTCAATTCATTTTGTACTCGTCAACTGTTGACTGCTATGCATATTTGAGGAATTGAAGACCCATTTGCTTTCCAAGATGGAGGGATCGACCAAGAACGTCTTACTTTCTTCCGGTGGGGATGAGATCTCCAAGAATCTGGCTCTTCATCTTGCCAGAAGGGGTTGCAGATTAGTTTTGATTGGAAATGAGTGTGTTCTTCAGAGCATGAGTAAGATGATAGCGGAATCGTTAAAGGGTGTACTGCCAATTGAGGTTGTTGGGGTGGACATGGAAGAAGAGAGAGAGGCGGCTTTTGATGAGGCTGTGAACAGGGCATGCGGTATTTTGGGAACTTTGGATGCTTTCGTCCATGCTTATTCTTATGAAGGTCCCATACAAGATGCTTTACAACTATCTGAAGAGGAATTCAAAAAGATTGTTAAGATAAATTTGATGGCCTCATGGTTTCTGATGAAGGCTGTTTGTCGAAGAATGCGAGACCAGAAATCAGGAGGTTCAGTAATTTTTTTAACCACCTTAATTGGTGCTGAGAGGGGATTGTATCCAGGAGGAGCTGCATATGGATCATGCTCAGCAGGCTTACAGCAGTTAGCTCGAACTTCAGCTCTGGATGTTGGAAAGTATAAGATCAGGGTGAACGCAATCGCTCGTGGATTGCACCTGGATGATGGATATCCTGTATCAGTGGGCAAGGAACGTGCAGAGAAGCAAGTCAAGGATGCAGCCCCGCTAGAAAGATGGCTCGATGTCAAGGATGATTTAGCTTCAACTGTCATTTATCTAATCAGCGATGGGTCACGGTACATGACTGGAACAACCATCTTTGTTGATGGGGCACAGTCTCTAGTGAGGCCACGCATGCGCTCATATATGTAGTCCTGGTTATATTTGGTGGATGGTCAGCCTTTGGGGGTGGATGTAGGCCATTGTTGATTCACTCAACCACTGTAACTGGAGCTGTTGTTCAACTAATGAATAATGTCCTCATAGGCTCTGTATTTGCTCGACCATTCTCGTGAATAAAGTCGAGGTATGCCCGGGGGTGCTTCCTTTAAATCAATTATTCTCTTCTTGATTCACTCAACCCATTGAGACTGTTTCCCCCTAGTACTCATGAATAAATTTGAAGTTCAGTGAAAGTATGTTTGAGTTGATCATTTCTTTAAAAAGCCTTTTTTTCTTCATTCATAGTTGAAGTTATCTCTTTTCCAAAATTCCAGTTTATAAATAGTGATTTCAAACTGTAATTTCAATTTTACAAGGGACTGCGAATGCAAAGCCCTTGGTCAGGGGCAAAGCAAAGGAAACAATGCAACATGAAATTTGGTTTGTGTATATATATACACTACAATAGGTGTAGGGTTCAAATTATAGACGTTTTGGTTGTTGATATGTCCAGTTGAGTTATGTGTTAGTAGCATCTAACACATCACATGCTCTGTGTGAGACAACTTGATGAATTTGAGTGGTGTTTGAAAGGCGAACTAGTCCAATAATTTCATGTGCCAAAATGTGGTTAAGCTTGTAGCAAAAAGCTTCTTGATTAATTAAATCAATTGTAAACAAACAAAAACAATTGGCATTGACATGAATTTG

Coding sequence (CDS)

ATGGAGGGATCGACCAAGAACGTCTTACTTTCTTCCGGTGGGGATGAGATCTCCAAGAATCTGGCTCTTCATCTTGCCAGAAGGGGTTGCAGATTAGTTTTGATTGGAAATGAGTGTGTTCTTCAGAGCATGAGTAAGATGATAGCGGAATCGTTAAAGGGTGTACTGCCAATTGAGGTTGTTGGGGTGGACATGGAAGAAGAGAGAGAGGCGGCTTTTGATGAGGCTGTGAACAGGGCATGCGGTATTTTGGGAACTTTGGATGCTTTCGTCCATGCTTATTCTTATGAAGGTCCCATACAAGATGCTTTACAACTATCTGAAGAGGAATTCAAAAAGATTGTTAAGATAAATTTGATGGCCTCATGGTTTCTGATGAAGGCTGTTTGTCGAAGAATGCGAGACCAGAAATCAGGAGGTTCAGTAATTTTTTTAACCACCTTAATTGGTGCTGAGAGGGGATTGTATCCAGGAGGAGCTGCATATGGATCATGCTCAGCAGGCTTACAGCAGTTAGCTCGAACTTCAGCTCTGGATGTTGGAAAGTATAAGATCAGGGTGAACGCAATCGCTCGTGGATTGCACCTGGATGATGGATATCCTGTATCAGTGGGCAAGGAACGTGCAGAGAAGCAAGTCAAGGATGCAGCCCCGCTAGAAAGATGGCTCGATGTCAAGGATGATTTAGCTTCAACTGTCATTTATCTAATCAGCGATGGGTCACGGTACATGACTGGAACAACCATCTTTGTTGATGGGGCACAGTCTCTAGTGAGGCCACGCATGCGCTCATATATGTAG

Protein sequence

MEGSTKNVLLSSGGDEISKNLALHLARRGCRLVLIGNECVLQSMSKMIAESLKGVLPIEVVGVDMEEEREAAFDEAVNRACGILGTLDAFVHAYSYEGPIQDALQLSEEEFKKIVKINLMASWFLMKAVCRRMRDQKSGGSVIFLTTLIGAERGLYPGGAAYGSCSAGLQQLARTSALDVGKYKIRVNAIARGLHLDDGYPVSVGKERAEKQVKDAAPLERWLDVKDDLASTVIYLISDGSRYMTGTTIFVDGAQSLVRPRMRSYM*

Homology

[Close]

BLAST of CSPI03G25410 vs. ExPASy Swiss-Prot
Match: Q9LBG2 (Levodione reductase OS=Leifsonia aquatica OX=144185 GN=lvr PE=1 SV=1)

HSP 1 Score: 90.9 bits (224), Expect = 2.4e-17
Identity = 71/260 (27.31%), Postives = 120/260 (46.15%), Query Frame = 0


Query: 8   VLLSSGGDEISKNLALHLARRGCRLVLIGNECVLQSMSKMIAESLKGVLPIEVVGVDMEE 67
           VL++ GG  + +  A+ LA  G +L L+  +   + +    A  L+     EV+    + 
Sbjct: 16  VLITGGGSGLGRATAVRLAAEGAKLSLV--DVSSEGLEASKAAVLETAPDAEVLTTVADV 75

Query: 68  EREAAFDEAVNRACGILGTLDAFVHAYSYEGPIQDALQLSEEEFKKIVKINLMASWFLMK 127
             EA  +  V       G +D F +    EG        +  EF K+V INL   +  ++
Sbjct: 76  SDEAQVEAYVTATTERFGRIDGFFNNAGIEGKQNPTESFTAAEFDKVVSINLRGVFLGLE 135

Query: 128 AVCRRMRDQKSGGSVIFLTTLIGAERGLYPGGAAYGSCSAGLQQLARTSALDVGKYKIRV 187
            V + MR+Q SG  ++  T  +G  RG+    + Y +   G+  L R SA++ G+Y IR+
Sbjct: 136 KVLKIMREQGSG--MVVNTASVGGIRGI-GNQSGYAAAKHGVVGLTRNSAVEYGRYGIRI 195

Query: 188 NAIARGL-----------HLDDGYPVSVGKERAEKQVKDAAPLERWLDVKDDLASTVIYL 247
           NAIA G             LD   P      +A ++     P +R+ +   ++A+ V +L
Sbjct: 196 NAIAPGAIWTPMVENSMKQLDPENP-----RKAAEEFIQVNPSKRYGEA-PEIAAVVAFL 255

Query: 248 ISDGSRYMTGTTIFVDGAQS 257
           +SD + Y+  T + +DG QS
Sbjct: 256 LSDDASYVNATVVPIDGGQS 264

[Close]

BLAST of CSPI03G25410 vs. ExPASy Swiss-Prot
Match: P9WGQ2 (Uncharacterized oxidoreductase MT1753.1 OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) OX=83331 GN=MT1753.1 PE=3 SV=1)

HSP 1 Score: 87.4 bits (215), Expect = 2.6e-16
Identity = 71/252 (28.17%), Postives = 120/252 (47.62%), Query Frame = 0


Query: 6   KNVLLSSGGDEISKNLALHLARRGCRLVLIGNECVLQSMSKMIAESLKGVLPIEVVGVDM 65
           K++L++     + +  A  LA  G RL L G        S  +AE + G    +   V  
Sbjct: 23  KSILITGATGSLGRVAARALADAGARLTLAGGN------SAGLAELVNGAGIDDAAVVTC 82

Query: 66  EEEREAAFDEAVNRACGILGTLDAFVHAYSYEGPIQDALQLSEEEFKKIVKINLMASWFL 125
             +  A   + V  A G  G LD  + A S    +    +++ E+F  ++  N+  +W +
Sbjct: 83  RPDSLADAQQMVEAALGRYGRLDGVLVA-SGSNHVAPITEMAVEDFDAVMDANVRGAWLV 142

Query: 126 MKAVCRRMRDQKSGGSVIFLTTLIGAERGLYPGGAAYGSCSAGLQQLARTSALDVGKYKI 185
            +A  R + +Q  GGSV+ ++++ G   G   G +AY    AG   LA+T A + G + I
Sbjct: 143 CRAAGRVLLEQGQGGSVVLVSSVRGG-LGNAAGYSAYCPSKAGTDLLAKTLAAEWGGHGI 202

Query: 186 RVNAIARGLH---LDDGYPVSVGKERAEKQVKDA-APLERWLDVKDDLASTVIYLISDGS 245
           RVNA+A  +    + +       K RA ++   A  PL R+ +  +D    +IYL+SD S
Sbjct: 203 RVNALAPTVFRSAVTEWMFTDDPKGRATREAMLARIPLRRFAE-PEDFVGALIYLLSDAS 262

Query: 246 RYMTGTTIFVDG 254
            + TG  +++DG
Sbjct: 263 SFYTGQVMYLDG 265

[Close]

BLAST of CSPI03G25410 vs. ExPASy Swiss-Prot
Match: P9WGQ3 (Uncharacterized oxidoreductase Rv1714 OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=Rv1714 PE=3 SV=1)

HSP 1 Score: 87.4 bits (215), Expect = 2.6e-16
Identity = 71/252 (28.17%), Postives = 120/252 (47.62%), Query Frame = 0


Query: 6   KNVLLSSGGDEISKNLALHLARRGCRLVLIGNECVLQSMSKMIAESLKGVLPIEVVGVDM 65
           K++L++     + +  A  LA  G RL L G        S  +AE + G    +   V  
Sbjct: 23  KSILITGATGSLGRVAARALADAGARLTLAGGN------SAGLAELVNGAGIDDAAVVTC 82

Query: 66  EEEREAAFDEAVNRACGILGTLDAFVHAYSYEGPIQDALQLSEEEFKKIVKINLMASWFL 125
             +  A   + V  A G  G LD  + A S    +    +++ E+F  ++  N+  +W +
Sbjct: 83  RPDSLADAQQMVEAALGRYGRLDGVLVA-SGSNHVAPITEMAVEDFDAVMDANVRGAWLV 142

Query: 126 MKAVCRRMRDQKSGGSVIFLTTLIGAERGLYPGGAAYGSCSAGLQQLARTSALDVGKYKI 185
            +A  R + +Q  GGSV+ ++++ G   G   G +AY    AG   LA+T A + G + I
Sbjct: 143 CRAAGRVLLEQGQGGSVVLVSSVRGG-LGNAAGYSAYCPSKAGTDLLAKTLAAEWGGHGI 202

Query: 186 RVNAIARGLH---LDDGYPVSVGKERAEKQVKDA-APLERWLDVKDDLASTVIYLISDGS 245
           RVNA+A  +    + +       K RA ++   A  PL R+ +  +D    +IYL+SD S
Sbjct: 203 RVNALAPTVFRSAVTEWMFTDDPKGRATREAMLARIPLRRFAE-PEDFVGALIYLLSDAS 262

Query: 246 RYMTGTTIFVDG 254
            + TG  +++DG
Sbjct: 263 SFYTGQVMYLDG 265

[Close]

BLAST of CSPI03G25410 vs. ExPASy Swiss-Prot
Match: Q29529 (Carbonyl reductase [NADPH] 2 OS=Sus scrofa OX=9823 GN=CBR2 PE=1 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 1.4e-14
Identity = 71/254 (27.95%), Postives = 132/254 (51.97%), Query Frame = 0


Query: 1   MEGSTKNVLLSSGGDEISKNLALHLARRGCRLVLIGNECVLQSMSKMIAESLKGVLP-IE 60
           M  S    L++  G  I ++    L   G R+V      V ++   ++  SL    P IE
Sbjct: 3   MNFSGLRALVTGAGKGIGRDTVKALHVSGARVV-----AVTRTNGDLV--SLSQECPGIE 62

Query: 61  VVGVDMEEEREAAFDEAVNRACGILGTLDAFVHAYSYEGPIQDALQLSEEEFKKIVKINL 120
            V VD+ +       EA  RA G +G +D  V+  +    +Q  L  ++E F +   +NL
Sbjct: 63  PVCVDLGDW------EATERALGGVGPVDLLVNNAAV-ALMQPFLDTTKEVFDRSFNVNL 122

Query: 121 MASWFLMKAVCRRMRDQKSGGSVIFLTTLIGAERGLYPGGAAYGSCSAGLQQLARTSALD 180
            + + + + V R M ++   GS++ +++++      YPG AAY S    +  L ++ A++
Sbjct: 123 RSVFQVSQIVARSMIERGVPGSIVNVSSMV--SHVTYPGLAAYSSTKGAMTMLTKSMAME 182

Query: 181 VGKYKIRVNAIARGLHLDD-GYPVSVGKERAEKQVKDAAPLERWLDVKDDLASTVIYLIS 240
           +G +KIRVN++   + L   G  V+   E A K +K+  P+ ++ +V +D+ +++++L+S
Sbjct: 183 LGPHKIRVNSVNPTVVLTAMGRSVTSDPELARK-LKERHPMRKFAEV-EDVVNSILFLLS 238

Query: 241 DGSRYMTGTTIFVD 253
           D S   +G++IFVD
Sbjct: 243 DRSASTSGSSIFVD 238

[Close]

BLAST of CSPI03G25410 vs. ExPASy Swiss-Prot
Match: P32573 (Peroxisomal 2,4-dienoyl-CoA reductase SPS19 [(3E)-enoyl-CoA-producing] OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPS19 PE=1 SV=4)

HSP 1 Score: 79.7 bits (195), Expect = 5.5e-14
Identity = 72/253 (28.46%), Postives = 118/253 (46.64%), Query Frame = 0


Query: 6   KNVLLSSGGDEISKNLALHLARRGCRLVLIGNECVLQSMS----KMIAESLKGVLPIEVV 65
           K   ++ G   I +     L   GC+  ++G +      +      +A+    VL I  V
Sbjct: 25  KVAFVTGGAGTICRVQTEALVLLGCKAAIVGRDQERTEQAAKGISQLAKDKDAVLAIANV 84

Query: 66  GVDMEEEREAAFDEAVNRACGILGTLDAFVHAYSYEGPIQDALQLSEEEFKKIVKINLMA 125
            V   E+ E A  + V +     G +D FV A +    + D   LS   FK +V I+L+ 
Sbjct: 85  DVRNFEQVENAVKKTVEK----FGKID-FVIAGAAGNFVCDFANLSPNAFKSVVDIDLLG 144

Query: 126 SWFLMKAVCRRMRDQKSGGSVIFLTTLIGAERGLYPGGAAYGSCSAGLQQLARTSALDVG 185
           S+   KA  + ++  KS GS++F++         + G    G+  AG+  LA+  A+++G
Sbjct: 145 SFNTAKACLKELK--KSKGSILFVSATFHYYGVPFQGHV--GAAKAGIDALAKNLAVELG 204

Query: 186 KYKIRVNAIARG-LHLDDGYPVSVGKERAEKQVKDAAPLERWLDVKDDLASTVIYLISDG 245
              IR N IA G +   +G     GK+  EK +    PL+R L    D+A + +Y+ S  
Sbjct: 205 PLGIRSNCIAPGAIDNTEGLKRLAGKKYKEKALA-KIPLQR-LGSTRDIAESTVYIFSPA 264

Query: 246 SRYMTGTTIFVDG 254
           + Y+TGT + VDG
Sbjct: 265 ASYVTGTVLVVDG 266

[Close]

BLAST of CSPI03G25410 vs. ExPASy TrEMBL
Match: A0A0A0L8L9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G566330 PE=4 SV=1)

HSP 1 Score: 523.9 bits (1348), Expect = 4.0e-145
Identity = 266/266 (100.00%), Postives = 266/266 (100.00%), Query Frame = 0


Query: 1   MEGSTKNVLLSSGGDEISKNLALHLARRGCRLVLIGNECVLQSMSKMIAESLKGVLPIEV 60
           MEGSTKNVLLSSGGDEISKNLALHLARRGCRLVLIGNECVLQSMSKMIAESLKGVLPIEV
Sbjct: 1   MEGSTKNVLLSSGGDEISKNLALHLARRGCRLVLIGNECVLQSMSKMIAESLKGVLPIEV 60

Query: 61  VGVDMEEEREAAFDEAVNRACGILGTLDAFVHAYSYEGPIQDALQLSEEEFKKIVKINLM 120
           VGVDMEEEREAAFDEAVNRACGILGTLDAFVHAYSYEGPIQDALQLSEEEFKKIVKINLM
Sbjct: 61  VGVDMEEEREAAFDEAVNRACGILGTLDAFVHAYSYEGPIQDALQLSEEEFKKIVKINLM 120

Query: 121 ASWFLMKAVCRRMRDQKSGGSVIFLTTLIGAERGLYPGGAAYGSCSAGLQQLARTSALDV 180
           ASWFLMKAVCRRMRDQKSGGSVIFLTTLIGAERGLYPGGAAYGSCSAGLQQLARTSALDV
Sbjct: 121 ASWFLMKAVCRRMRDQKSGGSVIFLTTLIGAERGLYPGGAAYGSCSAGLQQLARTSALDV 180

Query: 181 GKYKIRVNAIARGLHLDDGYPVSVGKERAEKQVKDAAPLERWLDVKDDLASTVIYLISDG 240
           GKYKIRVNAIARGLHLDDGYPVSVGKERAEKQVKDAAPLERWLDVKDDLASTVIYLISDG
Sbjct: 181 GKYKIRVNAIARGLHLDDGYPVSVGKERAEKQVKDAAPLERWLDVKDDLASTVIYLISDG 240

Query: 241 SRYMTGTTIFVDGAQSLVRPRMRSYM 267
           SRYMTGTTIFVDGAQSLVRPRMRSYM
Sbjct: 241 SRYMTGTTIFVDGAQSLVRPRMRSYM 266

[Close]

BLAST of CSPI03G25410 vs. ExPASy TrEMBL
Match: Q2PZA4 (Putative alcohol dehydrogenases OS=Cucumis melo OX=3656 GN=ADH2 PE=2 SV=1)

HSP 1 Score: 509.6 bits (1311), Expect = 7.9e-141
Identity = 258/266 (96.99%), Postives = 263/266 (98.87%), Query Frame = 0


Query: 1   MEGSTKNVLLSSGGDEISKNLALHLARRGCRLVLIGNECVLQSMSKMIAESLKGVLPIEV 60
           MEG+TKNVLLSSGGDEISKNLALHLARRGCRLVLIGNECVLQSMSKMIAESLKGVLPIEV
Sbjct: 1   MEGATKNVLLSSGGDEISKNLALHLARRGCRLVLIGNECVLQSMSKMIAESLKGVLPIEV 60

Query: 61  VGVDMEEEREAAFDEAVNRACGILGTLDAFVHAYSYEGPIQDALQLSEEEFKKIVKINLM 120
           VG+DMEEEREAAFDEAVNRAC +LGTLDAFVHAYSYEGPIQDALQLSEEEFKKIVKINLM
Sbjct: 61  VGLDMEEEREAAFDEAVNRACSVLGTLDAFVHAYSYEGPIQDALQLSEEEFKKIVKINLM 120

Query: 121 ASWFLMKAVCRRMRDQKSGGSVIFLTTLIGAERGLYPGGAAYGSCSAGLQQLARTSALDV 180
           ASWFLMKAVCRRMRDQKSGGSVIFLTTLIGAERGLYPGGAAYGSCSAGLQQLARTSALDV
Sbjct: 121 ASWFLMKAVCRRMRDQKSGGSVIFLTTLIGAERGLYPGGAAYGSCSAGLQQLARTSALDV 180

Query: 181 GKYKIRVNAIARGLHLDDGYPVSVGKERAEKQVKDAAPLERWLDVKDDLASTVIYLISDG 240
           GKYKIRVNAIARGLHLD+GYPVSVGKERA+K VKDAA LERWLDVKDDLASTVIYLISDG
Sbjct: 181 GKYKIRVNAIARGLHLDNGYPVSVGKERAKKLVKDAAKLERWLDVKDDLASTVIYLISDG 240

Query: 241 SRYMTGTTIFVDGAQSLVRPRMRSYM 267
           SRYMTGTTIFVDGAQSLVRPRMRSYM
Sbjct: 241 SRYMTGTTIFVDGAQSLVRPRMRSYM 266

[Close]

BLAST of CSPI03G25410 vs. ExPASy TrEMBL
Match: A0A5A7V0A0 (Carbonyl reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold639G00030 PE=4 SV=1)

HSP 1 Score: 495.7 bits (1275), Expect = 1.2e-136
Identity = 251/257 (97.67%), Postives = 255/257 (99.22%), Query Frame = 0


Query: 1   MEGSTKNVLLSSGGDEISKNLALHLARRGCRLVLIGNECVLQSMSKMIAESLKGVLPIEV 60
           MEG+TKNVLLSSGGDEISKNLALHLARRGCRLVLIGNECVLQSMSKMIAESLKGVLPIEV
Sbjct: 1   MEGATKNVLLSSGGDEISKNLALHLARRGCRLVLIGNECVLQSMSKMIAESLKGVLPIEV 60

Query: 61  VGVDMEEEREAAFDEAVNRACGILGTLDAFVHAYSYEGPIQDALQLSEEEFKKIVKINLM 120
           VG+DMEEEREAAFDEAVNRAC +LGTLDAFVHAYSYEGPIQDALQLSEEEFKKIVKINLM
Sbjct: 61  VGLDMEEEREAAFDEAVNRACSVLGTLDAFVHAYSYEGPIQDALQLSEEEFKKIVKINLM 120

Query: 121 ASWFLMKAVCRRMRDQKSGGSVIFLTTLIGAERGLYPGGAAYGSCSAGLQQLARTSALDV 180
           ASWFLMKAVCRRMRDQKSGGSVIFLTTLIGAERGLYPGGAAYGSCSAGLQQLARTSALDV
Sbjct: 121 ASWFLMKAVCRRMRDQKSGGSVIFLTTLIGAERGLYPGGAAYGSCSAGLQQLARTSALDV 180

Query: 181 GKYKIRVNAIARGLHLDDGYPVSVGKERAEKQVKDAAPLERWLDVKDDLASTVIYLISDG 240
           GKYKIRVNAIARGLHLDDGYPVSVGKERA+K VKDAAPLERWLDVKDDLASTVIYLISDG
Sbjct: 181 GKYKIRVNAIARGLHLDDGYPVSVGKERAKKLVKDAAPLERWLDVKDDLASTVIYLISDG 240

Query: 241 SRYMTGTTIFVDGAQSL 258
           SRYMTGTTIFVDGAQSL
Sbjct: 241 SRYMTGTTIFVDGAQSL 257

[Close]

BLAST of CSPI03G25410 vs. ExPASy TrEMBL
Match: A0A6J1EJP8 (carbonyl reductase [NADPH] 2-like OS=Cucurbita moschata OX=3662 GN=LOC111435110 PE=4 SV=1)

HSP 1 Score: 466.8 bits (1200), Expect = 5.9e-128
Identity = 230/266 (86.47%), Postives = 252/266 (94.74%), Query Frame = 0


Query: 1   MEGSTKNVLLSSGGDEISKNLALHLARRGCRLVLIGNECVLQSMSKMIAESLKGVLPIEV 60
           MEG+TKNVLLSS GDEISKNLA+HLA+RGCRLVL+GNE VL+SM + I ESLKG LPI V
Sbjct: 1   MEGATKNVLLSSNGDEISKNLAIHLAKRGCRLVLVGNETVLRSMREGIVESLKGALPIGV 60

Query: 61  VGVDMEEEREAAFDEAVNRACGILGTLDAFVHAYSYEGPIQDALQLSEEEFKKIVKINLM 120
           VG+DME EREAAFDEAV+ AC +LGTLDAFVHAYSYEGPIQ+ LQLSEEEFKKIVKIN+M
Sbjct: 61  VGLDMEGEREAAFDEAVSGACSVLGTLDAFVHAYSYEGPIQEVLQLSEEEFKKIVKINMM 120

Query: 121 ASWFLMKAVCRRMRDQKSGGSVIFLTTLIGAERGLYPGGAAYGSCSAGLQQLARTSALDV 180
           ASWFLMKAVCRRMRDQKSGGS++FL++LIG+ERGLYPG AA+GSCSAGLQQLART+ALDV
Sbjct: 121 ASWFLMKAVCRRMRDQKSGGSIVFLSSLIGSERGLYPGAAAFGSCSAGLQQLARTAALDV 180

Query: 181 GKYKIRVNAIARGLHLDDGYPVSVGKERAEKQVKDAAPLERWLDVKDDLASTVIYLISDG 240
           GKYKIRVNAI+RGLHLDDGYP+SVG ERA+K VKDAAPLERWLD+KDDLASTVIYLISDG
Sbjct: 181 GKYKIRVNAISRGLHLDDGYPISVGTERAKKLVKDAAPLERWLDIKDDLASTVIYLISDG 240

Query: 241 SRYMTGTTIFVDGAQSLVRPRMRSYM 267
           SRYMTGTTIFVDGAQSLVRPRMRSYM
Sbjct: 241 SRYMTGTTIFVDGAQSLVRPRMRSYM 266

[Close]

BLAST of CSPI03G25410 vs. ExPASy TrEMBL
Match: A0A6J1JR50 (carbonyl reductase [NADPH] 2-like OS=Cucurbita maxima OX=3661 GN=LOC111486709 PE=4 SV=1)

HSP 1 Score: 462.6 bits (1189), Expect = 1.1e-126
Identity = 228/266 (85.71%), Postives = 250/266 (93.98%), Query Frame = 0


Query: 1   MEGSTKNVLLSSGGDEISKNLALHLARRGCRLVLIGNECVLQSMSKMIAESLKGVLPIEV 60
           ME +TKNVLLSS GDEISKNLA+HLA+RGCRLVL+GNE VL SM + I ESLKG LPI V
Sbjct: 1   MERATKNVLLSSNGDEISKNLAIHLAKRGCRLVLVGNETVLHSMREEIGESLKGALPIGV 60

Query: 61  VGVDMEEEREAAFDEAVNRACGILGTLDAFVHAYSYEGPIQDALQLSEEEFKKIVKINLM 120
           VG+DME EREAAFDEAV+ AC +LGTLDAFVH+YSYEGPIQ+ LQLSEEEFKKIVKIN+M
Sbjct: 61  VGLDMEGEREAAFDEAVSGACSVLGTLDAFVHSYSYEGPIQEVLQLSEEEFKKIVKINMM 120

Query: 121 ASWFLMKAVCRRMRDQKSGGSVIFLTTLIGAERGLYPGGAAYGSCSAGLQQLARTSALDV 180
           +SWFLMKAVCRRMRDQKSGGS++FL++LIG+ERGLYPG AA+GSCSAGLQQLART+ALDV
Sbjct: 121 SSWFLMKAVCRRMRDQKSGGSIVFLSSLIGSERGLYPGAAAFGSCSAGLQQLARTAALDV 180

Query: 181 GKYKIRVNAIARGLHLDDGYPVSVGKERAEKQVKDAAPLERWLDVKDDLASTVIYLISDG 240
           GKYKIRVNAIARGLHLDDGYP+SVG ERA+K VKDAAPLERWLD+KDDLASTVIYLISDG
Sbjct: 181 GKYKIRVNAIARGLHLDDGYPISVGTERAKKLVKDAAPLERWLDIKDDLASTVIYLISDG 240

Query: 241 SRYMTGTTIFVDGAQSLVRPRMRSYM 267
           SRYMTGTTIFVDGAQSLVRPRMRSYM
Sbjct: 241 SRYMTGTTIFVDGAQSLVRPRMRSYM 266

[Close]

BLAST of CSPI03G25410 vs. NCBI nr
Match: XP_011651549.1 (uncharacterized protein LOC101219109 [Cucumis sativus] >KGN58163.1 hypothetical protein Csa_017656 [Cucumis sativus])

HSP 1 Score: 523.9 bits (1348), Expect = 8.3e-145
Identity = 266/266 (100.00%), Postives = 266/266 (100.00%), Query Frame = 0


Query: 1   MEGSTKNVLLSSGGDEISKNLALHLARRGCRLVLIGNECVLQSMSKMIAESLKGVLPIEV 60
           MEGSTKNVLLSSGGDEISKNLALHLARRGCRLVLIGNECVLQSMSKMIAESLKGVLPIEV
Sbjct: 1   MEGSTKNVLLSSGGDEISKNLALHLARRGCRLVLIGNECVLQSMSKMIAESLKGVLPIEV 60

Query: 61  VGVDMEEEREAAFDEAVNRACGILGTLDAFVHAYSYEGPIQDALQLSEEEFKKIVKINLM 120
           VGVDMEEEREAAFDEAVNRACGILGTLDAFVHAYSYEGPIQDALQLSEEEFKKIVKINLM
Sbjct: 61  VGVDMEEEREAAFDEAVNRACGILGTLDAFVHAYSYEGPIQDALQLSEEEFKKIVKINLM 120

Query: 121 ASWFLMKAVCRRMRDQKSGGSVIFLTTLIGAERGLYPGGAAYGSCSAGLQQLARTSALDV 180
           ASWFLMKAVCRRMRDQKSGGSVIFLTTLIGAERGLYPGGAAYGSCSAGLQQLARTSALDV
Sbjct: 121 ASWFLMKAVCRRMRDQKSGGSVIFLTTLIGAERGLYPGGAAYGSCSAGLQQLARTSALDV 180

Query: 181 GKYKIRVNAIARGLHLDDGYPVSVGKERAEKQVKDAAPLERWLDVKDDLASTVIYLISDG 240
           GKYKIRVNAIARGLHLDDGYPVSVGKERAEKQVKDAAPLERWLDVKDDLASTVIYLISDG
Sbjct: 181 GKYKIRVNAIARGLHLDDGYPVSVGKERAEKQVKDAAPLERWLDVKDDLASTVIYLISDG 240

Query: 241 SRYMTGTTIFVDGAQSLVRPRMRSYM 267
           SRYMTGTTIFVDGAQSLVRPRMRSYM
Sbjct: 241 SRYMTGTTIFVDGAQSLVRPRMRSYM 266

[Close]

BLAST of CSPI03G25410 vs. NCBI nr
Match: NP_001284466.1 (carbonyl reductase [NADPH] 2 [Cucumis melo] >ABC02082.1 putative alcohol dehydrogenases [Cucumis melo])

HSP 1 Score: 509.6 bits (1311), Expect = 1.6e-140
Identity = 258/266 (96.99%), Postives = 263/266 (98.87%), Query Frame = 0


Query: 1   MEGSTKNVLLSSGGDEISKNLALHLARRGCRLVLIGNECVLQSMSKMIAESLKGVLPIEV 60
           MEG+TKNVLLSSGGDEISKNLALHLARRGCRLVLIGNECVLQSMSKMIAESLKGVLPIEV
Sbjct: 1   MEGATKNVLLSSGGDEISKNLALHLARRGCRLVLIGNECVLQSMSKMIAESLKGVLPIEV 60

Query: 61  VGVDMEEEREAAFDEAVNRACGILGTLDAFVHAYSYEGPIQDALQLSEEEFKKIVKINLM 120
           VG+DMEEEREAAFDEAVNRAC +LGTLDAFVHAYSYEGPIQDALQLSEEEFKKIVKINLM
Sbjct: 61  VGLDMEEEREAAFDEAVNRACSVLGTLDAFVHAYSYEGPIQDALQLSEEEFKKIVKINLM 120

Query: 121 ASWFLMKAVCRRMRDQKSGGSVIFLTTLIGAERGLYPGGAAYGSCSAGLQQLARTSALDV 180
           ASWFLMKAVCRRMRDQKSGGSVIFLTTLIGAERGLYPGGAAYGSCSAGLQQLARTSALDV
Sbjct: 121 ASWFLMKAVCRRMRDQKSGGSVIFLTTLIGAERGLYPGGAAYGSCSAGLQQLARTSALDV 180

Query: 181 GKYKIRVNAIARGLHLDDGYPVSVGKERAEKQVKDAAPLERWLDVKDDLASTVIYLISDG 240
           GKYKIRVNAIARGLHLD+GYPVSVGKERA+K VKDAA LERWLDVKDDLASTVIYLISDG
Sbjct: 181 GKYKIRVNAIARGLHLDNGYPVSVGKERAKKLVKDAAKLERWLDVKDDLASTVIYLISDG 240

Query: 241 SRYMTGTTIFVDGAQSLVRPRMRSYM 267
           SRYMTGTTIFVDGAQSLVRPRMRSYM
Sbjct: 241 SRYMTGTTIFVDGAQSLVRPRMRSYM 266

[Close]

BLAST of CSPI03G25410 vs. NCBI nr
Match: KAA0060247.1 (carbonyl reductase [Cucumis melo var. makuwa] >TYJ97611.1 carbonyl reductase [Cucumis melo var. makuwa])

HSP 1 Score: 495.7 bits (1275), Expect = 2.4e-136
Identity = 251/257 (97.67%), Postives = 255/257 (99.22%), Query Frame = 0


Query: 1   MEGSTKNVLLSSGGDEISKNLALHLARRGCRLVLIGNECVLQSMSKMIAESLKGVLPIEV 60
           MEG+TKNVLLSSGGDEISKNLALHLARRGCRLVLIGNECVLQSMSKMIAESLKGVLPIEV
Sbjct: 1   MEGATKNVLLSSGGDEISKNLALHLARRGCRLVLIGNECVLQSMSKMIAESLKGVLPIEV 60

Query: 61  VGVDMEEEREAAFDEAVNRACGILGTLDAFVHAYSYEGPIQDALQLSEEEFKKIVKINLM 120
           VG+DMEEEREAAFDEAVNRAC +LGTLDAFVHAYSYEGPIQDALQLSEEEFKKIVKINLM
Sbjct: 61  VGLDMEEEREAAFDEAVNRACSVLGTLDAFVHAYSYEGPIQDALQLSEEEFKKIVKINLM 120

Query: 121 ASWFLMKAVCRRMRDQKSGGSVIFLTTLIGAERGLYPGGAAYGSCSAGLQQLARTSALDV 180
           ASWFLMKAVCRRMRDQKSGGSVIFLTTLIGAERGLYPGGAAYGSCSAGLQQLARTSALDV
Sbjct: 121 ASWFLMKAVCRRMRDQKSGGSVIFLTTLIGAERGLYPGGAAYGSCSAGLQQLARTSALDV 180

Query: 181 GKYKIRVNAIARGLHLDDGYPVSVGKERAEKQVKDAAPLERWLDVKDDLASTVIYLISDG 240
           GKYKIRVNAIARGLHLDDGYPVSVGKERA+K VKDAAPLERWLDVKDDLASTVIYLISDG
Sbjct: 181 GKYKIRVNAIARGLHLDDGYPVSVGKERAKKLVKDAAPLERWLDVKDDLASTVIYLISDG 240

Query: 241 SRYMTGTTIFVDGAQSL 258
           SRYMTGTTIFVDGAQSL
Sbjct: 241 SRYMTGTTIFVDGAQSL 257

[Close]

BLAST of CSPI03G25410 vs. NCBI nr
Match: XP_038878089.1 (levodione reductase-like [Benincasa hispida])

HSP 1 Score: 482.6 bits (1241), Expect = 2.1e-132
Identity = 244/266 (91.73%), Postives = 257/266 (96.62%), Query Frame = 0


Query: 1   MEGSTKNVLLSSGGDEISKNLALHLARRGCRLVLIGNECVLQSMSKMIAESLKGVLPIEV 60
           ME +TKNVLLSS GD+ISKNLA+HLA+RGCRLVLIGNE VLQSMSK++AESLKGVLPIEV
Sbjct: 20  MERATKNVLLSSNGDDISKNLAIHLAKRGCRLVLIGNEKVLQSMSKVMAESLKGVLPIEV 79

Query: 61  VGVDMEEEREAAFDEAVNRACGILGTLDAFVHAYSYEGPIQDALQLSEEEFKKIVKINLM 120
           VG+DMEEEREAAFD+AVNRAC ILGTLDAF HAYSYEGPIQDALQLSEEEF+KIV+INLM
Sbjct: 80  VGLDMEEEREAAFDKAVNRACSILGTLDAFAHAYSYEGPIQDALQLSEEEFQKIVRINLM 139

Query: 121 ASWFLMKAVCRRMRDQKSGGSVIFLTTLIGAERGLYPGGAAYGSCSAGLQQLARTSALDV 180
           ASWFLMKAVCRRMRDQKSGGSVIFLTTLIGAERGLY GGAAYGSC AGLQQLART ALDV
Sbjct: 140 ASWFLMKAVCRRMRDQKSGGSVIFLTTLIGAERGLYQGGAAYGSCLAGLQQLARTLALDV 199

Query: 181 GKYKIRVNAIARGLHLDDGYPVSVGKERAEKQVKDAAPLERWLDVKDDLASTVIYLISDG 240
           GKYKIRVNAIARGLHLDDGYPVSVGKERA+K VK+AAPLERWLDVK+DLASTVIYLISDG
Sbjct: 200 GKYKIRVNAIARGLHLDDGYPVSVGKERAKKLVKEAAPLERWLDVKEDLASTVIYLISDG 259

Query: 241 SRYMTGTTIFVDGAQSLVRPRMRSYM 267
           SRYMTGTTIFVDGAQSLVRPRMRSYM
Sbjct: 260 SRYMTGTTIFVDGAQSLVRPRMRSYM 285

[Close]

BLAST of CSPI03G25410 vs. NCBI nr
Match: XP_023529292.1 (carbonyl reductase [NADPH] 2-like [Cucurbita pepo subsp. pepo] >XP_023529293.1 carbonyl reductase [NADPH] 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 468.8 bits (1205), Expect = 3.2e-128
Identity = 232/266 (87.22%), Postives = 252/266 (94.74%), Query Frame = 0


Query: 1   MEGSTKNVLLSSGGDEISKNLALHLARRGCRLVLIGNECVLQSMSKMIAESLKGVLPIEV 60
           ME +TKNVLLSS GDEISKNLA+HLA+RGCRLVL+GNE VLQSM + I ESLKGVLPI V
Sbjct: 1   MERATKNVLLSSNGDEISKNLAIHLAKRGCRLVLVGNETVLQSMREGIVESLKGVLPIGV 60

Query: 61  VGVDMEEEREAAFDEAVNRACGILGTLDAFVHAYSYEGPIQDALQLSEEEFKKIVKINLM 120
           VG+DME EREAAFDEAVN AC +LGTLDAFVHAYSYEGPIQ+ LQLSEEEFKKIVKIN+M
Sbjct: 61  VGLDMEGEREAAFDEAVNGACSVLGTLDAFVHAYSYEGPIQEVLQLSEEEFKKIVKINMM 120

Query: 121 ASWFLMKAVCRRMRDQKSGGSVIFLTTLIGAERGLYPGGAAYGSCSAGLQQLARTSALDV 180
           ASWFLMKAVCRRMRDQKSGGS++FL++LIG+ERGLYPG AA+GSCSAGLQQLART+ALDV
Sbjct: 121 ASWFLMKAVCRRMRDQKSGGSIVFLSSLIGSERGLYPGAAAFGSCSAGLQQLARTAALDV 180

Query: 181 GKYKIRVNAIARGLHLDDGYPVSVGKERAEKQVKDAAPLERWLDVKDDLASTVIYLISDG 240
           GKYKIRVNAI+RGLHLDDGYP+SVG ERA+K VKDAAPLERWLD+KDDLASTVIYLISDG
Sbjct: 181 GKYKIRVNAISRGLHLDDGYPISVGTERAKKLVKDAAPLERWLDIKDDLASTVIYLISDG 240

Query: 241 SRYMTGTTIFVDGAQSLVRPRMRSYM 267
           SRYMTGTTIFVDGAQSLVRPRMRSYM
Sbjct: 241 SRYMTGTTIFVDGAQSLVRPRMRSYM 266

[Close]

BLAST of CSPI03G25410 vs. TAIR 10
Match: AT3G01980.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 322.0 bits (824), Expect = 4.5e-88
Identity = 152/266 (57.14%), Postives = 202/266 (75.94%), Query Frame = 0


Query: 1   MEGSTKNVLLSSGGDEISKNLALHLARRGCRLVLIGNECVLQSMSKMIAESLKGVLPIEV 60
           ME   K VL++S GDE+S+N+A HLA+ GC+LV++GNE  L+S+   I +S++G  P +V
Sbjct: 1   MENPAKRVLMTSNGDEVSRNIAFHLAKHGCKLVMMGNEGSLRSIVDKIRDSIEGAFPADV 60

Query: 61  VGVDMEEEREAAFDEAVNRACGILGTLDAFVHAYSYEGPIQDALQLSEEEFKKIVKINLM 120
           + +DME + E AF  AV +A  + G  DAF+++Y+Y+G +QD LQ+S++EF +I KINL 
Sbjct: 61  IALDMESDSEVAFHAAVQKAWELSGHFDAFLNSYTYQGKVQDILQVSQDEFHRITKINLT 120

Query: 121 ASWFLMKAVCRRMRDQKSGGSVIFLTTLIGAERGLYPGGAAYGSCSAGLQQLARTSALDV 180
           A WFL+KAV  RM+D  SGGS++F+ T+   ER LYPG  AY S SA + QL R SA+ +
Sbjct: 121 APWFLLKAVATRMKDHGSGGSIVFMATIASGERALYPGADAYASTSAAIHQLVRASAMSL 180

Query: 181 GKYKIRVNAIARGLHLDDGYPVSVGKERAEKQVKDAAPLERWLDVKDDLASTVIYLISDG 240
           GK+KIRVN I+RGLHLDD Y  SVG++RA+K VKDAAPL +WL+   DL STVIYLISDG
Sbjct: 181 GKHKIRVNMISRGLHLDDEYTASVGRDRAQKLVKDAAPLGQWLNPDTDLYSTVIYLISDG 240

Query: 241 SRYMTGTTIFVDGAQSLVRPRMRSYM 267
           SR+MTGTT+ VDGAQSL RPR++SYM
Sbjct: 241 SRFMTGTTVLVDGAQSLTRPRLKSYM 266

[Close]

BLAST of CSPI03G25410 vs. TAIR 10
Match: AT3G01980.4 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 322.0 bits (824), Expect = 4.5e-88
Identity = 152/266 (57.14%), Postives = 202/266 (75.94%), Query Frame = 0


Query: 1   MEGSTKNVLLSSGGDEISKNLALHLARRGCRLVLIGNECVLQSMSKMIAESLKGVLPIEV 60
           ME   K VL++S GDE+S+N+A HLA+ GC+LV++GNE  L+S+   I +S++G  P +V
Sbjct: 1   MENPAKRVLMTSNGDEVSRNIAFHLAKHGCKLVMMGNEGSLRSIVDKIRDSIEGAFPADV 60

Query: 61  VGVDMEEEREAAFDEAVNRACGILGTLDAFVHAYSYEGPIQDALQLSEEEFKKIVKINLM 120
           + +DME + E AF  AV +A  + G  DAF+++Y+Y+G +QD LQ+S++EF +I KINL 
Sbjct: 61  IALDMESDSEVAFHAAVQKAWELSGHFDAFLNSYTYQGKVQDILQVSQDEFHRITKINLT 120

Query: 121 ASWFLMKAVCRRMRDQKSGGSVIFLTTLIGAERGLYPGGAAYGSCSAGLQQLARTSALDV 180
           A WFL+KAV  RM+D  SGGS++F+ T+   ER LYPG  AY S SA + QL R SA+ +
Sbjct: 121 APWFLLKAVATRMKDHGSGGSIVFMATIASGERALYPGADAYASTSAAIHQLVRASAMSL 180

Query: 181 GKYKIRVNAIARGLHLDDGYPVSVGKERAEKQVKDAAPLERWLDVKDDLASTVIYLISDG 240
           GK+KIRVN I+RGLHLDD Y  SVG++RA+K VKDAAPL +WL+   DL STVIYLISDG
Sbjct: 181 GKHKIRVNMISRGLHLDDEYTASVGRDRAQKLVKDAAPLGQWLNPDTDLYSTVIYLISDG 240

Query: 241 SRYMTGTTIFVDGAQSLVRPRMRSYM 267
           SR+MTGTT+ VDGAQSL RPR++SYM
Sbjct: 241 SRFMTGTTVLVDGAQSLTRPRLKSYM 266

[Close]

BLAST of CSPI03G25410 vs. TAIR 10
Match: AT3G01980.3 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 306.2 bits (783), Expect = 2.5e-83
Identity = 152/296 (51.35%), Postives = 202/296 (68.24%), Query Frame = 0


Query: 1   MEGSTKNVLLSSGGDEISKNLALHLARRGCRLVLIGNECVLQSMSKMIAESLKGVLPIEV 60
           ME   K VL++S GDE+S+N+A HLA+ GC+LV++GNE  L+S+   I +S++G  P +V
Sbjct: 1   MENPAKRVLMTSNGDEVSRNIAFHLAKHGCKLVMMGNEGSLRSIVDKIRDSIEGAFPADV 60

Query: 61  VGVDMEEEREAAFDEAVNRACGILGTLDAFVHAYSYE----------------------- 120
           + +DME + E AF  AV +A  + G  DAF+++Y+Y+                       
Sbjct: 61  IALDMESDSEVAFHAAVQKAWELSGHFDAFLNSYTYQGLICFLFFTTLPLMLLCVDHSFI 120

Query: 121 -------GPIQDALQLSEEEFKKIVKINLMASWFLMKAVCRRMRDQKSGGSVIFLTTLIG 180
                  G +QD LQ+S++EF +I KINL A WFL+KAV  RM+D  SGGS++F+ T+  
Sbjct: 121 QQSFFLAGKVQDILQVSQDEFHRITKINLTAPWFLLKAVATRMKDHGSGGSIVFMATIAS 180

Query: 181 AERGLYPGGAAYGSCSAGLQQLARTSALDVGKYKIRVNAIARGLHLDDGYPVSVGKERAE 240
            ER LYPG  AY S SA + QL R SA+ +GK+KIRVN I+RGLHLDD Y  SVG++RA+
Sbjct: 181 GERALYPGADAYASTSAAIHQLVRASAMSLGKHKIRVNMISRGLHLDDEYTASVGRDRAQ 240

Query: 241 KQVKDAAPLERWLDVKDDLASTVIYLISDGSRYMTGTTIFVDGAQSLVRPRMRSYM 267
           K VKDAAPL +WL+   DL STVIYLISDGSR+MTGTT+ VDGAQSL RPR++SYM
Sbjct: 241 KLVKDAAPLGQWLNPDTDLYSTVIYLISDGSRFMTGTTVLVDGAQSLTRPRLKSYM 296

[Close]

BLAST of CSPI03G25410 vs. TAIR 10
Match: AT3G01980.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 174.1 bits (440), Expect = 1.5e-43
Identity = 88/208 (42.31%), Postives = 125/208 (60.10%), Query Frame = 0


Query: 1   MEGSTKNVLLSSGGDEISKNLALHLARRGCRLVLIGNECVLQSMSKMIAESLKGVLPIEV 60
           ME   K VL++S GDE+S+N+A HLA+ GC+LV++GNE  L+S+   I +S++G  P +V
Sbjct: 1   MENPAKRVLMTSNGDEVSRNIAFHLAKHGCKLVMMGNEGSLRSIVDKIRDSIEGAFPADV 60

Query: 61  VGVDMEEEREAAFDEAVNRACGILGTLDAFVHAYSYE----------------------- 120
           + +DME + E AF  AV +A  + G  DAF+++Y+Y+                       
Sbjct: 61  IALDMESDSEVAFHAAVQKAWELSGHFDAFLNSYTYQGLICFLFFTTLPLMLLCVDHSFI 120

Query: 121 -------GPIQDALQLSEEEFKKIVKINLMASWFLMKAVCRRMRDQKSGGSVIFLTTLIG 179
                  G +QD LQ+S++EF +I KINL A WFL+KAV  RM+D  SGGS++F+ T+  
Sbjct: 121 QQSFFLAGKVQDILQVSQDEFHRITKINLTAPWFLLKAVATRMKDHGSGGSIVFMATIAS 180

[Close]

BLAST of CSPI03G25410 vs. TAIR 10
Match: AT1G62610.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 145.6 bits (366), Expect = 5.8e-35
Identity = 84/254 (33.07%), Postives = 134/254 (52.76%), Query Frame = 0


Query: 6   KNVLLSSGGDEISKNLALHLARRGCRLVLIGNECVLQSMSKMIAE-SLKGVLPIEVVGVD 65
           K VL++     I + + L L + GC++V       +  ++ + +E +  G + ++   ++
Sbjct: 17  KVVLVTGASSGIGREICLDLCKAGCKIVAAARR--VDRLNSLCSEINSFGAIGVQAAALE 76

Query: 66  MEEEREA-AFDEAVNRACGILGTLDAFVHAYSYEGPIQDALQLSEEEFKKIVKINLMASW 125
           ++   +A    +AV  A  I GT+D  ++     G ++ +L LS+EE+ K+ + NL  SW
Sbjct: 77  LDVSSDADTIRKAVKEAWEIFGTIDVLINNAGIRGNVKSSLDLSKEEWDKVFRTNLTGSW 136

Query: 126 FLMKAVCRRMRDQKSGGSVIFLTTLIGAERGLYPGGAAYGSCSAGLQQLARTSALDVGKY 185
            + K VC  MRD K GGSVI ++++ G  RGL  GG AY     G+  + R  A+++  Y
Sbjct: 137 LISKYVCLLMRDAKRGGSVINVSSISGLHRGLLRGGLAYACSKGGVDTMTRMMAIELAVY 196

Query: 186 KIRVNAIARGLHLDDGYPVSVGKERAEKQVKDAAPLERWLDVKDDLASTVIYLISDGSRY 245
           KIRVN+IA G+   +       KE  EK  +   PL+    V   L S V YLI D S+Y
Sbjct: 197 KIRVNSIAPGIFRSEITQGLFQKEWLEKVTEKIVPLKMQQTVDPGLTSLVRYLIHDSSQY 256

Query: 246 MTGTTIFVDGAQSL 258
           +TG T  VD   +L
Sbjct: 257 VTGNTYIVDSGATL 268

The following BLAST results are available for this feature:

BLAST of CSPI03G25410 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LBG2	2.4e-17	27.31	Levodione reductase OS=Leifsonia aquatica OX=144185 GN=lvr PE=1 SV=1	[more]
P9WGQ2	2.6e-16	28.17	Uncharacterized oxidoreductase MT1753.1 OS=Mycobacterium tuberculosis (strain CD...	[more]
P9WGQ3	2.6e-16	28.17	Uncharacterized oxidoreductase Rv1714 OS=Mycobacterium tuberculosis (strain ATCC...	[more]
Q29529	1.4e-14	27.95	Carbonyl reductase [NADPH] 2 OS=Sus scrofa OX=9823 GN=CBR2 PE=1 SV=1	[more]
P32573	5.5e-14	28.46	Peroxisomal 2,4-dienoyl-CoA reductase SPS19 [(3E)-enoyl-CoA-producing] OS=Saccha...	[more]

BLAST of CSPI03G25410 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L8L9	4.0e-145	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G566330 PE=4 SV=1	[more]
Q2PZA4	7.9e-141	96.99	Putative alcohol dehydrogenases OS=Cucumis melo OX=3656 GN=ADH2 PE=2 SV=1	[more]
A0A5A7V0A0	1.2e-136	97.67	Carbonyl reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold639G0...	[more]
A0A6J1EJP8	5.9e-128	86.47	carbonyl reductase [NADPH] 2-like OS=Cucurbita moschata OX=3662 GN=LOC111435110 ...	[more]
A0A6J1JR50	1.1e-126	85.71	carbonyl reductase [NADPH] 2-like OS=Cucurbita maxima OX=3661 GN=LOC111486709 PE...	[more]

BLAST of CSPI03G25410 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011651549.1	8.3e-145	100.00	uncharacterized protein LOC101219109 [Cucumis sativus] >KGN58163.1 hypothetical ...	[more]
NP_001284466.1	1.6e-140	96.99	carbonyl reductase [NADPH] 2 [Cucumis melo] >ABC02082.1 putative alcohol dehydro...	[more]
KAA0060247.1	2.4e-136	97.67	carbonyl reductase [Cucumis melo var. makuwa] >TYJ97611.1 carbonyl reductase [Cu...	[more]
XP_038878089.1	2.1e-132	91.73	levodione reductase-like [Benincasa hispida]	[more]
XP_023529292.1	3.2e-128	87.22	carbonyl reductase [NADPH] 2-like [Cucurbita pepo subsp. pepo] >XP_023529293.1 c...	[more]

BLAST of CSPI03G25410 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G01980.1	4.5e-88	57.14	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT3G01980.4	4.5e-88	57.14	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT3G01980.3	2.5e-83	51.35	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT3G01980.2	1.5e-43	42.31	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT1G62610.1	5.8e-35	33.07	NAD(P)-binding Rossmann-fold superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.50.720		coord: 4..182 e-value: 1.1E-21 score: 79.2
None	No IPR available	PFAM	PF13561	adh_short_C2	coord: 17..177 e-value: 1.0E-17 score: 64.5
None	No IPR available	PANTHER	PTHR44375:SF6	F28J7.36 PROTEIN	coord: 1..179
None	No IPR available	PANTHER	PTHR44375	BETA-KETOACYL-ACP REDUCTASE-LIKE PROTEIN-RELATED	coord: 1..179
None	No IPR available	CDD	cd05233	SDR_c	coord: 11..175 e-value: 1.98512E-20 score: 83.102
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 6..179

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G25410.1	CSPI03G25410.1	mRNA
CSPI03G25410.3	CSPI03G25410.3	mRNA
CSPI03G25410.2	CSPI03G25410.2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0004090	carbonyl reductase (NADPH) activity
molecular_function	GO:0016491	oxidoreductase activity