CSPI03G25320 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI03G25320
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Membrane-anchored ubiquitin-fold protein
Location	Chr3: 22607765 .. 22611127 (+)
RNA-Seq Expression	CSPI03G25320
Synteny	CSPI03G25320

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTTATTTATTTATTATTAATAAAAATAAAAGCATAGAGAGAAAAAAAACCAAAATGAGAAGAAGGATTAATGGTATATTGTGATGGGAGCAAAATCTAGGGTTCTTTCTCATTTCAAGATCTCTTCCCGCGATTCTTTAACCTCTCTCTCTTCTCTTTAACAATTTCTCCATTTAAAAGATAAAGCTTCTGATTCCTTACATCTCTCTCCGCATCTTCTTTACTCAGGTATTCCCTTCCATCTACTCCTTTTTTTCTTTTTCTTTTTTTCTCTCTTTTCCTTTCTCATGATCTATGGTGATGATGCATCTTGTTTATCAATTTCAATTGTATTTAAATCCATTCATCCTCATCAATCTCTTTTCCATTTTCCTTCTTCCGCTTTGTTTACTTTCATTCCGTGTTATTCCTGAGTCAATTACGGTCTCAGCTTGTTTTCAGATGCGCTCTTTTTTTCCTTCTTTCTTCGGAATTCCCGGTTTCAGGTTTTGCTGTTTTTAAATTCATCTTGGTAGTGGTAGATTGCTTGGGGTGTTTTGTGTGGGAGGATTTTTCGGGGGAAGCTGCTACTGGCGATGAAGCTGGGTCTTTACTGGTAGACCTCAAATGCCGGACGAAGATTTGATCGAGCTTAAGTTCCGACTTTATGATGGATCGGATATCGGTCCATTTCGTTATTCCCCAACTTCAACTATAGCCATGGTTAAGGAGAGGATTGTTGCAGAATGGCCTAAAGGTCAGTGAATTGGATCATGTTATTAGTTCCATTTTGCTCTTGTGGTGTTTCAGTTTCAATTTGTTGAATTACTTTTGTATGCAATGCAACTGTCGAATCGTAAGCACGTATGCTGTATACTCTGCAGTAATTGTGTCTAATCCAACCTTGCTCTTTCAAAATTTGACTTCCTTGACTTGGGTACCCTCTAGCTCAACTTAGAAGTTGAACACTATCATACGCGAGAAAGACAACTTGTGACTTTTAATAAGAAATAACTGATTTCCCCCCAGCTATGAAGAAATACCGAAAGGTATGGATTCTGCCAGGAGTCTTGTATAGTATGTAAACTCTTCTCATGATGATACACAGAACTAAAGGTTAATCATTACCATCTTTGTCTTTGTTGAACATGCTGGAAATTTTTTTTTTACATTTTTTGGGGGTCTGTTCTGTTATTGGTTACATAAGAGTGAATACAGTGTCCAGCTTTATCTTCATGTCTTCCTTTACGGCTGAGTGAGTTGAAGTGCTATTGCTTCCAATTACTTTGCATCTCTCTATCTCGCTTCATTCTCCCGTCCATAGTGAAACTAACTACTGTAAATGTTTTTAATACTTTTTAGATAAGAAAGTTATACCCAAGGCAGCAAATGACGTAAAACTGATCAATGCTGGCAAGATTTTAGAAAACAACAAGACTGTTGGTCAATGTCGAGTGCCTTTTGGCGATCTTCCTAAAGGAGTTATTACTATGCATGTCGTGGTGCAGCCAACTATAGCAAAAGCAAAATCAGGTAGCCATCCTTTAAGTTTTAGATGTGCTTCTGCATTACCTTTAATACTCAGCCATTCTACTGATAACTACGTGATGTTATATATTATCTTCGTGTCAGTCATCTAGTATTTGTATGCTCCATTTATTTGGACTTTAGTCATTAGCAAAAGCAAAAAATATTTAAGATTTTGCAGGTAATGGGATATGCCTGATTTATTTATTCATAATTCATAATTGGCATCAATATTGTCGGGGTTTCCAGTGTTGTGATGATAACATGATATCCATGGGTGAGATTACCACTTAAGTTTAAACTAGTAAATGACAGTGTATACACACACATTACAGCGATTCTGATGATGTATAACATGATTGGGGGCAATTGTAATTTTCTTTGATTTATGTTCTAGTTTTTCATTGGTTTTTATCATTGGGTTTGGTCTACTTGGGTGCTTTAACATCCTCTATAAACCGGGATGAATTGTAATTTTACCTTTTGAATGGTAACAAAATTGAGTATTGATGACTTCTTAATGTTGTGTTTATTTGATCTTTAGCTTTTAATTCTTGTAAATTAAACTTTAATTGCTGTGATTAGATTTTTCATGGATTTGAAATAAATTAAAATTTCAATTGTGTTTTGATGACTTTAAATTTTGGTGTGCTGATGTAACCTCTCTTCTTGTCTAGAAATTGCCTGTGGAGGTTGGCAGTTTATTTCATACTCTTAGATGTAGGAGGCAGAATTTTTCTTTTCTTTTCTTTTCTTTTTTAAGGTTTTAACTATGCATCCGAGAGTTAAGAAATGTGACGGCCACAATACAATGAATAGTATTCCCTCATTTCTTCTCCTTCTCCCCAATTCCTCTTTTAGATCTTCGTTCAGGCCCCTCCTAATTTCTCTCTCATCTGTCCCCCTTTCTTTCTTTGTCCTGTTTTATCTCTTCGATTTCCTCTTATTGAGCTTTGAGGTTTCCATCGTTGCTTATGGGGATGCAACTTGGTTGCCTGTGGCATGAATCATGATCAGATGGAAAGAGGGGAGGAAGTGAAGAATGAAGCATAATCCTTTTGTAAAATGCGTACAATTAGTTTATCCTGTCAGCTTTTGCCTTTCAGTTAAGGACTATTAGGAGCTACTAGCTATTATAGAAAACCGATGCCAAACAAACATATCATAGGAAGAAAGTTTGAAAATATAGCGTACAAATCCGGGCCATTATGTAATTTACCTTAAAGTAAGACATTTGTAGGTATTTTGTGGAGTTTCATGTTTATAGATTGATGGGCGCATTAATTTTTGTTCACATGGGGATTCTTACTCTTTTGTTCCGTATGCTGACTACTAGACACTTAATTTGTTGTCCACAGAAAAGAAGGTCGACGAGACCCCAACAAAAAACGTATGTTCGTGTTCCATATTGTAGGAAGTACGGATGAATGAATGGTAATGAAGCTTGAGGTTTGCGAGGGGCTGGTTCCAGCCAGCCTAATTGGGGGTCGCATTAAAGTTGTAGCTTGCAAGAGAATATAATATCACTGTATAACAAAAGTTGGAATGCCTGATTAATTACATGGTTCATTTCTGGATGATCCTGCGTGACACGAATATGTAATGTCGTGTGAATCTATATATAGAATGAGTAGTAGTCTGAAGTATGTAATGTAGGTGGGTTTTGATTGTTTCAAAATTATTTTTACAGAAAGAGATGAGGGGTCGACTTTGATCCCGAGATTTGTATGTCATACATCTGAATTCAGCACGTGTAAAAACTAAACCAAACTGGACCTCCCATAATTTCTACAGTTAATGTTTTTGAGTCTTTACTCTTTTTGCTCTTTTCTTTTCGGATTCTTGTTTTTGAAACTT

mRNA sequence

TTTTATTTATTTATTATTAATAAAAATAAAAGCATAGAGAGAAAAAAAACCAAAATGAGAAGAAGGATTAATGGTATATTGTGATGGGAGCAAAATCTAGGGTTCTTTCTCATTTCAAGATCTCTTCCCGCGATTCTTTAACCTCTCTCTCTTCTCTTTAACAATTTCTCCATTTAAAAGATAAAGCTTCTGATTCCTTACATCTCTCTCCGCATCTTCTTTACTCAGTGGTAGATTGCTTGGGGTGTTTTGTGTGGGAGGATTTTTCGGGGGAAGCTGCTACTGGCGATGAAGCTGGGTCTTTACTGGTAGACCTCAAATGCCGGACGAAGATTTGATCGAGCTTAAGTTCCGACTTTATGATGGATCGGATATCGGTCCATTTCGTTATTCCCCAACTTCAACTATAGCCATGGTTAAGGAGAGGATTGTTGCAGAATGGCCTAAAGATAAGAAAGTTATACCCAAGGCAGCAAATGACGTAAAACTGATCAATGCTGGCAAGATTTTAGAAAACAACAAGACTGTTGGTCAATGTCGAGTGCCTTTTGGCGATCTTCCTAAAGGAGTTATTACTATGCATGTCGTGGTGCAGCCAACTATAGCAAAAGCAAAATCAGAAAAGAAGGTCGACGAGACCCCAACAAAAAACGTATGTTCGTGTTCCATATTGTAGGAAGTACGGATGAATGAATGGTAATGAAGCTTGAGGTTTGCGAGGGGCTGGTTCCAGCCAGCCTAATTGGGGGTCGCATTAAAGTTGTAGCTTGCAAGAGAATATAATATCACTGTATAACAAAAGTTGGAATGCCTGATTAATTACATGGTTCATTTCTGGATGATCCTGCGTGACACGAATATGTAATGTCGTGTGAATCTATATATAGAATGAGTAGTAGTCTGAAGTATGTAATGTAGGTGGGTTTTGATTGTTTCAAAATTATTTTTACAGAAAGAGATGAGGGGTCGACTTTGATCCCGAGATTTGTATGTCATACATCTGAATTCAGCACGTGTAAAAACTAAACCAAACTGGACCTCCCATAATTTCTACAGTTAATGTTTTTGAGTCTTTACTCTTTTTGCTCTTTTCTTTTCGGATTCTTGTTTTTGAAACTT

Coding sequence (CDS)

ATGCCGGACGAAGATTTGATCGAGCTTAAGTTCCGACTTTATGATGGATCGGATATCGGTCCATTTCGTTATTCCCCAACTTCAACTATAGCCATGGTTAAGGAGAGGATTGTTGCAGAATGGCCTAAAGATAAGAAAGTTATACCCAAGGCAGCAAATGACGTAAAACTGATCAATGCTGGCAAGATTTTAGAAAACAACAAGACTGTTGGTCAATGTCGAGTGCCTTTTGGCGATCTTCCTAAAGGAGTTATTACTATGCATGTCGTGGTGCAGCCAACTATAGCAAAAGCAAAATCAGAAAAGAAGGTCGACGAGACCCCAACAAAAAACGTATGTTCGTGTTCCATATTGTAG

Protein sequence

MPDEDLIELKFRLYDGSDIGPFRYSPTSTIAMVKERIVAEWPKDKKVIPKAANDVKLINAGKILENNKTVGQCRVPFGDLPKGVITMHVVVQPTIAKAKSEKKVDETPTKNVCSCSIL*

Homology

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BLAST of CSPI03G25320 vs. ExPASy Swiss-Prot
Match: Q9LSD8 (Membrane-anchored ubiquitin-fold protein 4 OS=Arabidopsis thaliana OX=3702 GN=MUB4 PE=1 SV=1)

HSP 1 Score: 186.4 bits (472), Expect = 1.8e-46
Identity = 83/120 (69.17%), Postives = 106/120 (88.33%), Query Frame = 0


Query: 1   MPDEDLIELKFRLYDGSDIGPFRYSPTSTIAMVKERIVAEWPKDKKVIPKAANDVKLINA 60
           MP+EDL+ELKFRLYDGSD+GPF+YSPT+T++M+KERIV+EWPKDKK++PK+A+D+KLINA
Sbjct: 1   MPEEDLVELKFRLYDGSDVGPFQYSPTATVSMLKERIVSEWPKDKKIVPKSASDIKLINA 60

Query: 61  GKILENNKTVGQCRVPFGDLPKGVITMHVVVQPTIAKAKSEKKV--DETPTKNVCSCSIL 119
           GKILEN KTV QC+ PF DLPK VITMHVVVQ +  KA+ EKK+  +E P ++ CSC+I+
Sbjct: 61  GKILENGKTVAQCKAPFDDLPKSVITMHVVVQLSPTKARPEKKIEKEEAPQRSFCSCTIM 120

[Close]

BLAST of CSPI03G25320 vs. ExPASy Swiss-Prot
Match: Q9SW27 (Membrane-anchored ubiquitin-fold protein 3 OS=Arabidopsis thaliana OX=3702 GN=MUB3 PE=1 SV=1)

HSP 1 Score: 177.2 bits (448), Expect = 1.1e-43
Identity = 77/118 (65.25%), Postives = 101/118 (85.59%), Query Frame = 0


Query: 1   MPDEDLIELKFRLYDGSDIGPFRYSPTSTIAMVKERIVAEWPKDKKVIPKAANDVKLINA 60
           MP+E+ I++KFRLYDGSDIGPFRYS  ST+  +K+R+V++WPK K V+PK  N+VKLI++
Sbjct: 1   MPEEESIDIKFRLYDGSDIGPFRYSAASTVDFLKQRVVSDWPKGKTVVPKGINEVKLISS 60

Query: 61  GKILENNKTVGQCRVPFGDLPKGVITMHVVVQPTIAKAKSEKKVDETPTKNVCSCSIL 119
           GKILENNKTVGQC+ PFGD+  GVI MHVVVQP++AK+K+EKKVD+ P   +C+C+IL
Sbjct: 61  GKILENNKTVGQCKTPFGDIAGGVIVMHVVVQPSLAKSKTEKKVDKAPKAVICTCTIL 118

[Close]

BLAST of CSPI03G25320 vs. ExPASy Swiss-Prot
Match: Q7XRU4 (Membrane-anchored ubiquitin-fold protein 4 OS=Oryza sativa subsp. japonica OX=39947 GN=MUB4 PE=2 SV=2)

HSP 1 Score: 154.1 bits (388), Expect = 1.0e-36
Identity = 69/116 (59.48%), Postives = 92/116 (79.31%), Query Frame = 0


Query: 4   EDLIELKFRLYDGSDIGPFRYSP-TSTIAMVKERIVAEWPKDKKVIPKAANDVKLINAGK 63
           ++ +E+KFRL+DGSDIGP R +   +T+A +K+R+VA+WPKDK ++PK ANDVKLI+ GK
Sbjct: 20  DEEVEVKFRLFDGSDIGPLRCNAVATTVAALKDRVVADWPKDKTIVPKTANDVKLISGGK 79

Query: 64  ILENNKTVGQCRVPFGDLPKGVITMHVVVQPTIAKAKSEKKVDETPTKNVCSCSIL 119
           ILEN+K + QCR PFGDLP   ITMHVVVQP+ AK+K +KK ++ P    CSC+IL
Sbjct: 80  ILENDKNIAQCRAPFGDLPSTAITMHVVVQPSSAKSKPDKKTNKLPKTTRCSCTIL 135

[Close]

BLAST of CSPI03G25320 vs. ExPASy Swiss-Prot
Match: Q6Z8K4 (Membrane-anchored ubiquitin-fold protein 3 OS=Oryza sativa subsp. japonica OX=39947 GN=MUB3 PE=3 SV=1)

HSP 1 Score: 144.8 bits (364), Expect = 6.2e-34
Identity = 63/112 (56.25%), Postives = 89/112 (79.46%), Query Frame = 0


Query: 7   IELKFRLYDGSDIGPFRYSPTSTIAMVKERIVAEWPKDKKVIPKAANDVKLINAGKILEN 66
           IE+KFRL+DG+DIGP +Y P++T++ +KE I+A WP+DK++ PK  ND+KLINAG+ILEN
Sbjct: 8   IEVKFRLFDGTDIGPSKYDPSTTVSALKEFILARWPQDKEITPKTVNDLKLINAGRILEN 67

Query: 67  NKTVGQCRVPFGDLPKGVITMHVVVQPTIAKAKSEKKVDETPTKNVCSCSIL 119
           N+T+ + RVP G++P GVITMHVVV+P      SEK++  +P +N C C+IL
Sbjct: 68  NRTLAESRVPVGEVPGGVITMHVVVRPPQPDKNSEKQLANSPKQNRCGCTIL 119

[Close]

BLAST of CSPI03G25320 vs. ExPASy Swiss-Prot
Match: Q9SH14 (Membrane-anchored ubiquitin-fold protein 5 OS=Arabidopsis thaliana OX=3702 GN=MUB5 PE=1 SV=1)

HSP 1 Score: 139.4 bits (350), Expect = 2.6e-32
Identity = 68/120 (56.67%), Postives = 88/120 (73.33%), Query Frame = 0


Query: 1   MPDEDLIELKFRLYDGSDIGPFRYSPTSTIAMVKERIVAEWPKDKKVIPKAANDVKLINA 60
           M DEDLIELKFRL DG+DIGP +YS   T+A +KE+I+A+WPKDK+  PK  N+VKLIN 
Sbjct: 1   MGDEDLIELKFRLADGTDIGPSKYSQFMTVASLKEKIIAQWPKDKENAPKMINEVKLING 60

Query: 61  GKILENNKTVGQCR--VPFGDLPKGVITMHVVVQPTIAKAKSEKKVDETPTKNVCSCSIL 119
           GKILENNKT+ + R  +  G+LP  V TMHVV++P + + K EK  ++ P K+ C C IL
Sbjct: 61  GKILENNKTLSEARSLITIGELPGIVTTMHVVLRPPLFEKKKEKLQNDPPRKSHCVCCIL 120

[Close]

BLAST of CSPI03G25320 vs. ExPASy TrEMBL
Match: A0A0A0L887 (Membrane-anchored ubiquitin-fold protein OS=Cucumis sativus OX=3659 GN=Csa_3G563280 PE=4 SV=1)

HSP 1 Score: 242.7 bits (618), Expect = 8.0e-61
Identity = 118/118 (100.00%), Postives = 118/118 (100.00%), Query Frame = 0


Query: 1   MPDEDLIELKFRLYDGSDIGPFRYSPTSTIAMVKERIVAEWPKDKKVIPKAANDVKLINA 60
           MPDEDLIELKFRLYDGSDIGPFRYSPTSTIAMVKERIVAEWPKDKKVIPKAANDVKLINA
Sbjct: 1   MPDEDLIELKFRLYDGSDIGPFRYSPTSTIAMVKERIVAEWPKDKKVIPKAANDVKLINA 60

Query: 61  GKILENNKTVGQCRVPFGDLPKGVITMHVVVQPTIAKAKSEKKVDETPTKNVCSCSIL 119
           GKILENNKTVGQCRVPFGDLPKGVITMHVVVQPTIAKAKSEKKVDETPTKNVCSCSIL
Sbjct: 61  GKILENNKTVGQCRVPFGDLPKGVITMHVVVQPTIAKAKSEKKVDETPTKNVCSCSIL 118

[Close]

BLAST of CSPI03G25320 vs. ExPASy TrEMBL
Match: A0A1S3BNL5 (Membrane-anchored ubiquitin-fold protein OS=Cucumis melo OX=3656 GN=LOC103491812 PE=4 SV=1)

HSP 1 Score: 238.4 bits (607), Expect = 1.5e-59
Identity = 116/118 (98.31%), Postives = 117/118 (99.15%), Query Frame = 0


Query: 1   MPDEDLIELKFRLYDGSDIGPFRYSPTSTIAMVKERIVAEWPKDKKVIPKAANDVKLINA 60
           MPDEDLIELKFRLYDGSDIGPFRYSPTSTIAMVKERIVAEWPKDKKVIPKAANDVKLINA
Sbjct: 1   MPDEDLIELKFRLYDGSDIGPFRYSPTSTIAMVKERIVAEWPKDKKVIPKAANDVKLINA 60

Query: 61  GKILENNKTVGQCRVPFGDLPKGVITMHVVVQPTIAKAKSEKKVDETPTKNVCSCSIL 119
           GKILENNKTVGQCRVPFGDLP GVITMHVVVQPTIAKAK+EKKVDETPTKNVCSCSIL
Sbjct: 61  GKILENNKTVGQCRVPFGDLPIGVITMHVVVQPTIAKAKAEKKVDETPTKNVCSCSIL 118

[Close]

BLAST of CSPI03G25320 vs. ExPASy TrEMBL
Match: A0A6J1DS77 (Membrane-anchored ubiquitin-fold protein OS=Momordica charantia OX=3673 GN=LOC111023404 PE=4 SV=1)

HSP 1 Score: 233.0 bits (593), Expect = 6.3e-58
Identity = 110/118 (93.22%), Postives = 117/118 (99.15%), Query Frame = 0


Query: 1   MPDEDLIELKFRLYDGSDIGPFRYSPTSTIAMVKERIVAEWPKDKKVIPKAANDVKLINA 60
           MPDEDLIELKFRLYDGSDIGPFRYSPTSTIAMVKERI+AEWPKDKKV+PKAAND+KLINA
Sbjct: 1   MPDEDLIELKFRLYDGSDIGPFRYSPTSTIAMVKERILAEWPKDKKVVPKAANDIKLINA 60

Query: 61  GKILENNKTVGQCRVPFGDLPKGVITMHVVVQPTIAKAKSEKKVDETPTKNVCSCSIL 119
           GKILENNKTVGQCRVPFG+LP+GVITMHVVVQPTIAKAK+EKKVD+TP KNVCSCSIL
Sbjct: 61  GKILENNKTVGQCRVPFGELPRGVITMHVVVQPTIAKAKTEKKVDKTPRKNVCSCSIL 118

[Close]

BLAST of CSPI03G25320 vs. ExPASy TrEMBL
Match: A0A6J1ER66 (Membrane-anchored ubiquitin-fold protein OS=Cucurbita moschata OX=3662 GN=LOC111435138 PE=4 SV=1)

HSP 1 Score: 229.9 bits (585), Expect = 5.4e-57
Identity = 106/118 (89.83%), Postives = 117/118 (99.15%), Query Frame = 0


Query: 1   MPDEDLIELKFRLYDGSDIGPFRYSPTSTIAMVKERIVAEWPKDKKVIPKAANDVKLINA 60
           MPDEDLIELKFRLYDGSDIGP+RYSPTSTIA+VKE+IVA+WPKDKKV+PKAAND+KLINA
Sbjct: 1   MPDEDLIELKFRLYDGSDIGPYRYSPTSTIAVVKEKIVADWPKDKKVVPKAANDIKLINA 60

Query: 61  GKILENNKTVGQCRVPFGDLPKGVITMHVVVQPTIAKAKSEKKVDETPTKNVCSCSIL 119
           GK+LENNKTVGQCRVPFG+LP+GVITMHVVVQPTIAKAK+EKK+DETP KNVCSCSIL
Sbjct: 61  GKVLENNKTVGQCRVPFGELPRGVITMHVVVQPTIAKAKTEKKIDETPRKNVCSCSIL 118

[Close]

BLAST of CSPI03G25320 vs. ExPASy TrEMBL
Match: A0A6J1JL00 (Membrane-anchored ubiquitin-fold protein OS=Cucurbita maxima OX=3661 GN=LOC111486734 PE=4 SV=1)

HSP 1 Score: 227.6 bits (579), Expect = 2.7e-56
Identity = 104/118 (88.14%), Postives = 116/118 (98.31%), Query Frame = 0


Query: 1   MPDEDLIELKFRLYDGSDIGPFRYSPTSTIAMVKERIVAEWPKDKKVIPKAANDVKLINA 60
           MPD+DLIELKFRLYDGSDIGPFRYSPTSTIA+VKE+IVA+WPKDKKV+PKAAND+KLINA
Sbjct: 1   MPDQDLIELKFRLYDGSDIGPFRYSPTSTIAIVKEKIVADWPKDKKVVPKAANDIKLINA 60

Query: 61  GKILENNKTVGQCRVPFGDLPKGVITMHVVVQPTIAKAKSEKKVDETPTKNVCSCSIL 119
           GK+LENNKTVGQCRVPFG+LP+GVITMHVVVQPT+A AK+EKK+DETP KNVCSCSIL
Sbjct: 61  GKVLENNKTVGQCRVPFGELPRGVITMHVVVQPTVANAKTEKKIDETPRKNVCSCSIL 118

[Close]

BLAST of CSPI03G25320 vs. NCBI nr
Match: XP_004148006.2 (membrane-anchored ubiquitin-fold protein 4 [Cucumis sativus] >XP_011651547.1 membrane-anchored ubiquitin-fold protein 4 [Cucumis sativus] >XP_031737935.1 membrane-anchored ubiquitin-fold protein 4 [Cucumis sativus] >XP_031737936.1 membrane-anchored ubiquitin-fold protein 4 [Cucumis sativus] >XP_031737937.1 membrane-anchored ubiquitin-fold protein 4 [Cucumis sativus] >KGN58155.1 hypothetical protein Csa_017641 [Cucumis sativus])

HSP 1 Score: 242.7 bits (618), Expect = 1.7e-60
Identity = 118/118 (100.00%), Postives = 118/118 (100.00%), Query Frame = 0


Query: 1   MPDEDLIELKFRLYDGSDIGPFRYSPTSTIAMVKERIVAEWPKDKKVIPKAANDVKLINA 60
           MPDEDLIELKFRLYDGSDIGPFRYSPTSTIAMVKERIVAEWPKDKKVIPKAANDVKLINA
Sbjct: 1   MPDEDLIELKFRLYDGSDIGPFRYSPTSTIAMVKERIVAEWPKDKKVIPKAANDVKLINA 60

Query: 61  GKILENNKTVGQCRVPFGDLPKGVITMHVVVQPTIAKAKSEKKVDETPTKNVCSCSIL 119
           GKILENNKTVGQCRVPFGDLPKGVITMHVVVQPTIAKAKSEKKVDETPTKNVCSCSIL
Sbjct: 61  GKILENNKTVGQCRVPFGDLPKGVITMHVVVQPTIAKAKSEKKVDETPTKNVCSCSIL 118

[Close]

BLAST of CSPI03G25320 vs. NCBI nr
Match: XP_008450137.1 (PREDICTED: membrane-anchored ubiquitin-fold protein 4 [Cucumis melo] >XP_008450138.1 PREDICTED: membrane-anchored ubiquitin-fold protein 4 [Cucumis melo])

HSP 1 Score: 238.4 bits (607), Expect = 3.1e-59
Identity = 116/118 (98.31%), Postives = 117/118 (99.15%), Query Frame = 0


Query: 1   MPDEDLIELKFRLYDGSDIGPFRYSPTSTIAMVKERIVAEWPKDKKVIPKAANDVKLINA 60
           MPDEDLIELKFRLYDGSDIGPFRYSPTSTIAMVKERIVAEWPKDKKVIPKAANDVKLINA
Sbjct: 1   MPDEDLIELKFRLYDGSDIGPFRYSPTSTIAMVKERIVAEWPKDKKVIPKAANDVKLINA 60

Query: 61  GKILENNKTVGQCRVPFGDLPKGVITMHVVVQPTIAKAKSEKKVDETPTKNVCSCSIL 119
           GKILENNKTVGQCRVPFGDLP GVITMHVVVQPTIAKAK+EKKVDETPTKNVCSCSIL
Sbjct: 61  GKILENNKTVGQCRVPFGDLPIGVITMHVVVQPTIAKAKAEKKVDETPTKNVCSCSIL 118

[Close]

BLAST of CSPI03G25320 vs. NCBI nr
Match: XP_038905050.1 (membrane-anchored ubiquitin-fold protein 4-like [Benincasa hispida])

HSP 1 Score: 236.9 bits (603), Expect = 9.1e-59
Identity = 115/118 (97.46%), Postives = 116/118 (98.31%), Query Frame = 0


Query: 1   MPDEDLIELKFRLYDGSDIGPFRYSPTSTIAMVKERIVAEWPKDKKVIPKAANDVKLINA 60
           MPDEDLIELKFRLYDGSDIGPFRYSPTSTIAMVKERIVAEWPKDKKVIPKAANDVKLINA
Sbjct: 1   MPDEDLIELKFRLYDGSDIGPFRYSPTSTIAMVKERIVAEWPKDKKVIPKAANDVKLINA 60

Query: 61  GKILENNKTVGQCRVPFGDLPKGVITMHVVVQPTIAKAKSEKKVDETPTKNVCSCSIL 119
           GKILENNKTVGQCRVPFGDLPKGVITMHVVVQPTIAKAK+EKKVDETP K VCSCSIL
Sbjct: 61  GKILENNKTVGQCRVPFGDLPKGVITMHVVVQPTIAKAKTEKKVDETPRKTVCSCSIL 118

[Close]

BLAST of CSPI03G25320 vs. NCBI nr
Match: XP_022156522.1 (membrane-anchored ubiquitin-fold protein 4-like [Momordica charantia])

HSP 1 Score: 233.0 bits (593), Expect = 1.3e-57
Identity = 110/118 (93.22%), Postives = 117/118 (99.15%), Query Frame = 0


Query: 1   MPDEDLIELKFRLYDGSDIGPFRYSPTSTIAMVKERIVAEWPKDKKVIPKAANDVKLINA 60
           MPDEDLIELKFRLYDGSDIGPFRYSPTSTIAMVKERI+AEWPKDKKV+PKAAND+KLINA
Sbjct: 1   MPDEDLIELKFRLYDGSDIGPFRYSPTSTIAMVKERILAEWPKDKKVVPKAANDIKLINA 60

Query: 61  GKILENNKTVGQCRVPFGDLPKGVITMHVVVQPTIAKAKSEKKVDETPTKNVCSCSIL 119
           GKILENNKTVGQCRVPFG+LP+GVITMHVVVQPTIAKAK+EKKVD+TP KNVCSCSIL
Sbjct: 61  GKILENNKTVGQCRVPFGELPRGVITMHVVVQPTIAKAKTEKKVDKTPRKNVCSCSIL 118

[Close]

BLAST of CSPI03G25320 vs. NCBI nr
Match: XP_023530926.1 (membrane-anchored ubiquitin-fold protein 4-like [Cucurbita pepo subsp. pepo] >XP_023530927.1 membrane-anchored ubiquitin-fold protein 4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 231.5 bits (589), Expect = 3.8e-57
Identity = 107/118 (90.68%), Postives = 117/118 (99.15%), Query Frame = 0


Query: 1   MPDEDLIELKFRLYDGSDIGPFRYSPTSTIAMVKERIVAEWPKDKKVIPKAANDVKLINA 60
           MPDEDLIELKFRLYDGSDIGP+RYSPTSTIA+VKE+IVA+WPKDKKV+PKAAND+KLINA
Sbjct: 1   MPDEDLIELKFRLYDGSDIGPYRYSPTSTIAIVKEKIVADWPKDKKVVPKAANDIKLINA 60

Query: 61  GKILENNKTVGQCRVPFGDLPKGVITMHVVVQPTIAKAKSEKKVDETPTKNVCSCSIL 119
           GK+LENNKTVGQCRVPFGDLP+GVITMHVVVQPTIAKAK+EKK+DETP KNVCSCSIL
Sbjct: 61  GKVLENNKTVGQCRVPFGDLPRGVITMHVVVQPTIAKAKTEKKIDETPRKNVCSCSIL 118

[Close]

BLAST of CSPI03G25320 vs. TAIR 10
Match: AT3G26980.1 (membrane-anchored ubiquitin-fold protein 4 precursor )

HSP 1 Score: 186.4 bits (472), Expect = 1.3e-47
Identity = 83/120 (69.17%), Postives = 106/120 (88.33%), Query Frame = 0


Query: 1   MPDEDLIELKFRLYDGSDIGPFRYSPTSTIAMVKERIVAEWPKDKKVIPKAANDVKLINA 60
           MP+EDL+ELKFRLYDGSD+GPF+YSPT+T++M+KERIV+EWPKDKK++PK+A+D+KLINA
Sbjct: 1   MPEEDLVELKFRLYDGSDVGPFQYSPTATVSMLKERIVSEWPKDKKIVPKSASDIKLINA 60

Query: 61  GKILENNKTVGQCRVPFGDLPKGVITMHVVVQPTIAKAKSEKKV--DETPTKNVCSCSIL 119
           GKILEN KTV QC+ PF DLPK VITMHVVVQ +  KA+ EKK+  +E P ++ CSC+I+
Sbjct: 61  GKILENGKTVAQCKAPFDDLPKSVITMHVVVQLSPTKARPEKKIEKEEAPQRSFCSCTIM 120

[Close]

BLAST of CSPI03G25320 vs. TAIR 10
Match: AT4G24990.1 (Ubiquitin family protein )

HSP 1 Score: 177.2 bits (448), Expect = 8.0e-45
Identity = 77/118 (65.25%), Postives = 101/118 (85.59%), Query Frame = 0


Query: 1   MPDEDLIELKFRLYDGSDIGPFRYSPTSTIAMVKERIVAEWPKDKKVIPKAANDVKLINA 60
           MP+E+ I++KFRLYDGSDIGPFRYS  ST+  +K+R+V++WPK K V+PK  N+VKLI++
Sbjct: 1   MPEEESIDIKFRLYDGSDIGPFRYSAASTVDFLKQRVVSDWPKGKTVVPKGINEVKLISS 60

Query: 61  GKILENNKTVGQCRVPFGDLPKGVITMHVVVQPTIAKAKSEKKVDETPTKNVCSCSIL 119
           GKILENNKTVGQC+ PFGD+  GVI MHVVVQP++AK+K+EKKVD+ P   +C+C+IL
Sbjct: 61  GKILENNKTVGQCKTPFGDIAGGVIVMHVVVQPSLAKSKTEKKVDKAPKAVICTCTIL 118

[Close]

BLAST of CSPI03G25320 vs. TAIR 10
Match: AT1G77870.1 (membrane-anchored ubiquitin-fold protein 5 precursor )

HSP 1 Score: 139.4 bits (350), Expect = 1.8e-33
Identity = 68/120 (56.67%), Postives = 88/120 (73.33%), Query Frame = 0


Query: 1   MPDEDLIELKFRLYDGSDIGPFRYSPTSTIAMVKERIVAEWPKDKKVIPKAANDVKLINA 60
           M DEDLIELKFRL DG+DIGP +YS   T+A +KE+I+A+WPKDK+  PK  N+VKLIN 
Sbjct: 1   MGDEDLIELKFRLADGTDIGPSKYSQFMTVASLKEKIIAQWPKDKENAPKMINEVKLING 60

Query: 61  GKILENNKTVGQCR--VPFGDLPKGVITMHVVVQPTIAKAKSEKKVDETPTKNVCSCSIL 119
           GKILENNKT+ + R  +  G+LP  V TMHVV++P + + K EK  ++ P K+ C C IL
Sbjct: 61  GKILENNKTLSEARSLITIGELPGIVTTMHVVLRPPLFEKKKEKLQNDPPRKSHCVCCIL 120

[Close]

BLAST of CSPI03G25320 vs. TAIR 10
Match: AT1G22050.1 (membrane-anchored ubiquitin-fold protein 6 precursor )

HSP 1 Score: 136.3 bits (342), Expect = 1.6e-32
Identity = 64/117 (54.70%), Postives = 92/117 (78.63%), Query Frame = 0


Query: 3   DEDLIELKFRLYDGSDIGPFRYSPTSTIAMVKERIVAEWPKDKKVIPKAANDVKLINAGK 62
           +ED IELKFRL DG+DIGP +Y+ + T++ +KE+++++WPKDK+  PK  ND+KLINAGK
Sbjct: 4   EEDWIELKFRLADGTDIGPSKYNQSMTVSSLKEKLISQWPKDKENTPKTVNDMKLINAGK 63

Query: 63  ILENNKTVGQCRVPFGDLPKGVITMHVVVQ-PTIAKAKSEKKVDETPTKNVCSCSIL 119
           ILENN+T+ + R+P  +LP  +ITMH+V++ PT+ K KSEK  ++ P KN C C+IL
Sbjct: 64  ILENNRTLAESRLPVCELPGMIITMHIVLRLPTLDK-KSEKLQNDPPMKNRCVCTIL 119

[Close]

BLAST of CSPI03G25320 vs. TAIR 10
Match: AT5G15460.1 (membrane-anchored ubiquitin-fold protein 2 )

HSP 1 Score: 120.9 bits (302), Expect = 6.8e-28
Identity = 59/115 (51.30%), Postives = 77/115 (66.96%), Query Frame = 0


Query: 4   EDLIELKFRLYDGSDIGPFRYSPTSTIAMVKERIVAEWPKDKKVIPKAANDVKLINAGKI 63
           +D +E+KFRL DGSDIGP  +   +T+A +KE +VA+WP+DK+  PK   DVKLI+AG+I
Sbjct: 5   KDQLEIKFRLNDGSDIGPKLFPDATTVATLKETVVAQWPRDKENGPKTVKDVKLISAGRI 64

Query: 64  LENNKTVGQCRVPFGDLPKGVITMHVVVQPTIA---KAKSEKKVDETPTKNVCSC 116
           LENNKTVG CR P G+    V TMHV++Q  +    K K + K D    K VC C
Sbjct: 65  LENNKTVGDCRSPVGNFSGAVTTMHVIIQHQVTEKEKKKKKPKGDLKQNKCVCLC 119

The following BLAST results are available for this feature:

BLAST of CSPI03G25320 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LSD8	1.8e-46	69.17	Membrane-anchored ubiquitin-fold protein 4 OS=Arabidopsis thaliana OX=3702 GN=MU...	[more]
Q9SW27	1.1e-43	65.25	Membrane-anchored ubiquitin-fold protein 3 OS=Arabidopsis thaliana OX=3702 GN=MU...	[more]
Q7XRU4	1.0e-36	59.48	Membrane-anchored ubiquitin-fold protein 4 OS=Oryza sativa subsp. japonica OX=39...	[more]
Q6Z8K4	6.2e-34	56.25	Membrane-anchored ubiquitin-fold protein 3 OS=Oryza sativa subsp. japonica OX=39...	[more]
Q9SH14	2.6e-32	56.67	Membrane-anchored ubiquitin-fold protein 5 OS=Arabidopsis thaliana OX=3702 GN=MU...	[more]

BLAST of CSPI03G25320 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L887	8.0e-61	100.00	Membrane-anchored ubiquitin-fold protein OS=Cucumis sativus OX=3659 GN=Csa_3G563...	[more]
A0A1S3BNL5	1.5e-59	98.31	Membrane-anchored ubiquitin-fold protein OS=Cucumis melo OX=3656 GN=LOC103491812...	[more]
A0A6J1DS77	6.3e-58	93.22	Membrane-anchored ubiquitin-fold protein OS=Momordica charantia OX=3673 GN=LOC11...	[more]
A0A6J1ER66	5.4e-57	89.83	Membrane-anchored ubiquitin-fold protein OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]
A0A6J1JL00	2.7e-56	88.14	Membrane-anchored ubiquitin-fold protein OS=Cucurbita maxima OX=3661 GN=LOC11148...	[more]

BLAST of CSPI03G25320 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004148006.2	1.7e-60	100.00	membrane-anchored ubiquitin-fold protein 4 [Cucumis sativus] >XP_011651547.1 mem...	[more]
XP_008450137.1	3.1e-59	98.31	PREDICTED: membrane-anchored ubiquitin-fold protein 4 [Cucumis melo] >XP_0084501...	[more]
XP_038905050.1	9.1e-59	97.46	membrane-anchored ubiquitin-fold protein 4-like [Benincasa hispida]	[more]
XP_022156522.1	1.3e-57	93.22	membrane-anchored ubiquitin-fold protein 4-like [Momordica charantia]	[more]
XP_023530926.1	3.8e-57	90.68	membrane-anchored ubiquitin-fold protein 4-like [Cucurbita pepo subsp. pepo] >XP...	[more]

BLAST of CSPI03G25320 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G26980.1	1.3e-47	69.17	membrane-anchored ubiquitin-fold protein 4 precursor	[more]
AT4G24990.1	8.0e-45	65.25	Ubiquitin family protein	[more]
AT1G77870.1	1.8e-33	56.67	membrane-anchored ubiquitin-fold protein 5 precursor	[more]
AT1G22050.1	1.6e-32	54.70	membrane-anchored ubiquitin-fold protein 6 precursor	[more]
AT5G15460.1	6.8e-28	51.30	membrane-anchored ubiquitin-fold protein 2	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.10.20.90		coord: 1..100 e-value: 4.9E-45 score: 153.8
None	No IPR available	PANTHER	PTHR13169:SF1	MEMBRANE-ANCHORED UBIQUITIN-FOLD PROTEIN 4	coord: 1..118
None	No IPR available	CDD	cd01814	Ubl_MUBs_plant	coord: 6..93 e-value: 1.4871E-48 score: 148.296
IPR039540	UBL3-like, ubiquitin domain	PFAM	PF13881	Rad60-SLD_2	coord: 5..116 e-value: 1.5E-42 score: 144.2
IPR017000	Membrane-anchored ubiquitin-fold protein	PIRSF	PIRSF032572	MUB	coord: 1..118 e-value: 1.3E-50 score: 168.3
IPR040015	UBL3-like	PANTHER	PTHR13169	UBIQUITIN-LIKE PROTEIN 3 HCG-1 PROTEIN	coord: 1..118
IPR000626	Ubiquitin-like domain	PROSITE	PS50053	UBIQUITIN_2	coord: 7..75 score: 10.396963
IPR029071	Ubiquitin-like domain superfamily	SUPERFAMILY	54236	Ubiquitin-like	coord: 3..95

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G25320.1	CSPI03G25320.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0005515	protein binding