CSPI03G25060 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI03G25060
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Reverse transcriptase Ty1/copia-type domain-containing protein
Location	Chr3: 22281087 .. 22282403 (+)
RNA-Seq Expression	CSPI03G25060
Synteny	CSPI03G25060

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACAGGAAAAAGATGGAATAGAACTGATGTAGTTGTGGACAACATTTTTGTATATAATGTTGCACATAATATCACTCATGAAAATGAGGATTCTGAACCTAAATCTGTTGAAGAATGTTGTAATAGAAAGGATTGGCCCAAGTGGAAAGAAGTCATCCAAGCAAAATTGAACTCACTTTCAAAACGTGAAGTTTGTGGACCTGTAGTTCATACACCTAAAGGTGTAAAACCTATGGGATTTAAATGGGTATTTGTGCATAAAAGAAATGAAAATAATGAGGTCACTAGATATAAAGCACGACTTGTTGCACAAGGATTTTCTCAAAGACGGGGCATTGATTATGAGGAAACGTATTCTCATGTAGTGGATGTCATTACATTAAGATATCTAATTAGTCTGACTGTATGTGAAAAGCTTGATATGCATCTTATGGATGTAGTTACAACATATCTGTATGGATTTTTGGAAAATGAGATTTATATGAAAATCCCTGAAGGATTTAAGATACTTGAATCATATAATTCAAAATCTAGAGAATTATATTCAATCAAATTACAAAGATCATTATATGGATTGAAACAATCAGGACGAATGTGGTACAATCGCTTAAGTGAATATTTATTGAAGGAAGGTTATCAAAATAACCCAATTTGTCCATGTGTTTTTATTAAGAAATCACAGTCAAGATTTGCTATTATAGTTGTATATGTTGATGATTTAAATATAATTGGAACTCCTAAGAGCTTTCAAAGTCAATAGAATATTTCAAAAATGAATTCAAGATGAAAGATCTTAGCAAAACAAAATTTTGCCTTGGCTTGCAAATCGAGCATTTAGCTAATGGAATTTTTATTCATCAATCGACATATACATAAAAGATTTTAAAAAGATTCTACATGGACAAAGCACACCCATTGAACATTTCAATGGTGGTTCGATCACTAGATGTGAAAAGGGACATCTTTCGACCTCAAGAAGATAATGAAGAATTACTTGGTTCTGAAGTACCATACTTTAGTGCAATAGGTGCACTAATGTATCTTGCTAATAACACGAGACCATATATAGCATTTTCAGTAAATTTATTAGCAAGATATAATTCTTCTCCAACGAAAAGACATTGGAATGAAATTAAGCATGTACTTCGTTATCTTCGAGGGGCAATTGATATGGTTTTGTTTTATTCAAACAAATCAAACTTTGATTTAGTTGGTTATGCAGATGTTGGATATTTATATGATCCACATAAAGCAAGATCTCAAACAGGTTATCTGTTTACATGTGGAGGAACGACTATATCTTGGCGATTTGTAA

mRNA sequence

ATGACAGGAAAAAGATGGAATAGAACTGATGTAGTTGTGGACAACATTTTTGTATATAATGTTGCACATAATATCACTCATGAAAATGAGGATTCTGAACCTAAATCTGTTGAAGAATGTTGTAATAGAAAGGATTGGCCCAAGTGGAAAGAAGTCATCCAAGCAAAATTGAACTCACTTTCAAAACGTGAAGTTTGTGGACCTGTAGTTCATACACCTAAAGGTGTAAAACCTATGGGATTTAAATGGGTATTTGTGCATAAAAGAAATGAAAATAATGAGGTCACTAGATATAAAGCACGACTTGTTGCACAAGGATTTTCTCAAAGACGGGGCATTGATTATGAGGAAACGTATTCTCATGTAGTGGATGTCATTACATTAAGATATCTAATTAGTCTGACTGTATGTGAAAAGCTTGATATGCATCTTATGGATGTAGTTACAACATATCTGTATGGATTTTTGGAAAATGAGATTTATATGAAAATCCCTGAAGGATTTAAGATACTTGAATCATATAATTCAAAATCTAGAGAATTATATTCAATCAAATTACAAAGATCATTATATGGATTGAAACAATCAGGACGAATGTGGTACAATCGCTTAAGTGAATATTTATTGAAGGAAGATGTGAAAAGGGACATCTTTCGACCTCAAGAAGATAATGAAGAATTACTTGGTTCTGAAATGTTGGATATTTATATGATCCACATAAAGCAAGATCTCAAACAGGTTATCTGTTTACATGTGGAGGAACGACTATATCTTGGCGATTTGTAA

Coding sequence (CDS)

ATGACAGGAAAAAGATGGAATAGAACTGATGTAGTTGTGGACAACATTTTTGTATATAATGTTGCACATAATATCACTCATGAAAATGAGGATTCTGAACCTAAATCTGTTGAAGAATGTTGTAATAGAAAGGATTGGCCCAAGTGGAAAGAAGTCATCCAAGCAAAATTGAACTCACTTTCAAAACGTGAAGTTTGTGGACCTGTAGTTCATACACCTAAAGGTGTAAAACCTATGGGATTTAAATGGGTATTTGTGCATAAAAGAAATGAAAATAATGAGGTCACTAGATATAAAGCACGACTTGTTGCACAAGGATTTTCTCAAAGACGGGGCATTGATTATGAGGAAACGTATTCTCATGTAGTGGATGTCATTACATTAAGATATCTAATTAGTCTGACTGTATGTGAAAAGCTTGATATGCATCTTATGGATGTAGTTACAACATATCTGTATGGATTTTTGGAAAATGAGATTTATATGAAAATCCCTGAAGGATTTAAGATACTTGAATCATATAATTCAAAATCTAGAGAATTATATTCAATCAAATTACAAAGATCATTATATGGATTGAAACAATCAGGACGAATGTGGTACAATCGCTTAAGTGAATATTTATTGAAGGAAGATGTGAAAAGGGACATCTTTCGACCTCAAGAAGATAATGAAGAATTACTTGGTTCTGAAATGTTGGATATTTATATGATCCACATAAAGCAAGATCTCAAACAGGTTATCTGTTTACATGTGGAGGAACGACTATATCTTGGCGATTTGTAA

Protein sequence

MTGKRWNRTDVVVDNIFVYNVAHNITHENEDSEPKSVEECCNRKDWPKWKEVIQAKLNSLSKREVCGPVVHTPKGVKPMGFKWVFVHKRNENNEVTRYKARLVAQGFSQRRGIDYEETYSHVVDVITLRYLISLTVCEKLDMHLMDVVTTYLYGFLENEIYMKIPEGFKILESYNSKSRELYSIKLQRSLYGLKQSGRMWYNRLSEYLLKEDVKRDIFRPQEDNEELLGSEMLDIYMIHIKQDLKQVICLHVEERLYLGDL*

Homology

[Close]

BLAST of CSPI03G25060 vs. ExPASy Swiss-Prot
Match: P10978 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 1.7e-28
Identity = 67/180 (37.22%), Postives = 113/180 (62.78%), Query Frame = 0


Query: 29  NEDSEPKSVEECCNRKDWPKWKEVIQAKLNSLSKREVCGPVVHTPKGVKPMGFKWVFVHK 88
           ++D EP+S++E  +  +  +  + +Q ++ SL K      +V  PKG +P+  KWVF  K
Sbjct: 806 SDDREPESLKEVLSHPEKNQLMKAMQEEMESLQKNGTY-KLVELPKGKRPLKCKWVFKLK 865

Query: 89  RNENNEVTRYKARLVAQGFSQRRGIDYEETYSHVVDVITLRYLISLTVCEKLDMHLMDVV 148
           ++ + ++ RYKARLV +GF Q++GID++E +S VV + ++R ++SL     L++  +DV 
Sbjct: 866 KDGDCKLVRYKARLVVKGFEQKKGIDFDEIFSPVVKMTSIRTILSLAASLDLEVEQLDVK 925

Query: 149 TTYLYGFLENEIYMKIPEGFKILESYNSKSRELYSIKLQRSLYGLKQSGRMWYNRLSEYL 208
           T +L+G LE EIYM+ PEGF++        ++    KL +SLYGLKQ+ R WY +   ++
Sbjct: 926 TAFLHGDLEEEIYMEQPEGFEV------AGKKHMVCKLNKSLYGLKQAPRQWYMKFDSFM 978

[Close]

BLAST of CSPI03G25060 vs. ExPASy Swiss-Prot
Match: P04146 (Copia protein OS=Drosophila melanogaster OX=7227 GN=GIP PE=1 SV=3)

HSP 1 Score: 115.9 bits (289), Expect = 6.7e-25
Identity = 70/178 (39.33%), Postives = 102/178 (57.30%), Query Frame = 0


Query: 34   PKSVEECCNRKDWPKWKEVIQAKLNSLSKREVCGPVVHTPKGVKPMGFKWVFVHKRNENN 93
            P S +E   R D   W+E I  +LN+  K      +   P+    +  +WVF  K NE  
Sbjct: 891  PNSFDEIQYRDDKSSWEEAINTELNA-HKINNTWTITKRPENKNIVDSRWVFSVKYNELG 950

Query: 94   EVTRYKARLVAQGFSQRRGIDYEETYSHVVDVITLRYLISLTVCEKLDMHLMDVVTTYLY 153
               RYKARLVA+GF+Q+  IDYEET++ V  + + R+++SL +   L +H MDV T +L 
Sbjct: 951  NPIRYKARLVARGFTQKYQIDYEETFAPVARISSFRFILSLVIQYNLKVHQMDVKTAFLN 1010

Query: 154  GFLENEIYMKIPEGFKILESYNSKSRELYSIKLQRSLYGLKQSGRMWYNRLSEYLLKE 212
            G L+ EIYM++P+G     S NS +      KL +++YGLKQ+ R W+  + E  LKE
Sbjct: 1011 GTLKEEIYMRLPQGI----SCNSDN----VCKLNKAIYGLKQAARCWF-EVFEQALKE 1058

[Close]

BLAST of CSPI03G25060 vs. ExPASy Swiss-Prot
Match: Q94HW2 (Retrovirus-related Pol polyprotein from transposon RE1 OS=Arabidopsis thaliana OX=3702 GN=RE1 PE=2 SV=1)

HSP 1 Score: 112.1 bits (279), Expect = 9.7e-24
Identity = 63/179 (35.20%), Postives = 100/179 (55.87%), Query Frame = 0


Query: 31   DSEPKSVEECCNRKDWPKWKEVIQAKLNSLSKREVCGPVVHTPKGVKPMGFKWVFVHKRN 90
            +SEP++  +    KD  +W+  + +++N+         V   P  V  +G +W+F  K N
Sbjct: 953  ESEPRTAIQAL--KD-ERWRNAMGSEINAQIGNHTWDLVPPPPSHVTIVGCRWIFTKKYN 1012

Query: 91   ENNEVTRYKARLVAQGFSQRRGIDYEETYSHVVDVITLRYLISLTVCEKLDMHLMDVVTT 150
             +  + RYKARLVA+G++QR G+DY ET+S V+   ++R ++ + V     +  +DV   
Sbjct: 1013 SDGSLNRYKARLVAKGYNQRPGLDYAETFSPVIKSTSIRIVLGVAVDRSWPIRQLDVNNA 1072

Query: 151  YLYGFLENEIYMKIPEGFKILESYNSKSRELYSIKLQRSLYGLKQSGRMWYNRLSEYLL 210
            +L G L +++YM  P GF        K R  Y  KL+++LYGLKQ+ R WY  L  YLL
Sbjct: 1073 FLQGTLTDDVYMSQPPGF------IDKDRPNYVCKLRKALYGLKQAPRAWYVELRNYLL 1122

[Close]

BLAST of CSPI03G25060 vs. ExPASy Swiss-Prot
Match: Q9ZT94 (Retrovirus-related Pol polyprotein from transposon RE2 OS=Arabidopsis thaliana OX=3702 GN=RE2 PE=4 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 1.7e-23
Identity = 62/179 (34.64%), Postives = 101/179 (56.42%), Query Frame = 0


Query: 31   DSEPKSVEECCNRKDWPKWKEVIQAKLNSLSKREVCGPVVHTPKGVKPMGFKWVFVHKRN 90
            +SEP++  +    KD  +W++ + +++N+         V   P  V  +G +W+F  K N
Sbjct: 936  NSEPRTAIQA--MKD-DRWRQAMGSEINAQIGNHTWDLVPPPPPSVTIVGCRWIFTKKFN 995

Query: 91   ENNEVTRYKARLVAQGFSQRRGIDYEETYSHVVDVITLRYLISLTVCEKLDMHLMDVVTT 150
             +  + RYKARLVA+G++QR G+DY ET+S V+   ++R ++ + V     +  +DV   
Sbjct: 996  SDGSLNRYKARLVAKGYNQRPGLDYAETFSPVIKSTSIRIVLGVAVDRSWPIRQLDVNNA 1055

Query: 151  YLYGFLENEIYMKIPEGFKILESYNSKSRELYSIKLQRSLYGLKQSGRMWYNRLSEYLL 210
            +L G L +E+YM  P GF        K R  Y  +L++++YGLKQ+ R WY  L  YLL
Sbjct: 1056 FLQGTLTDEVYMSQPPGFV------DKDRPDYVCRLRKAIYGLKQAPRAWYVELRTYLL 1105

[Close]

BLAST of CSPI03G25060 vs. ExPASy Swiss-Prot
Match: P92520 (Uncharacterized mitochondrial protein AtMg00820 OS=Arabidopsis thaliana OX=3702 GN=AtMg00820 PE=4 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 2.3e-09
Identity = 44/110 (40.00%), Postives = 64/110 (58.18%), Query Frame = 0


Query: 33  EPKSVEECCNRKDWPKWKEVIQAKLNSLSKREVCGPVVHTPKGVKPMGFKWVFVHKRNEN 92
           EPKSV      KD P W + +Q +L++LS+ +    +V  P     +G KWVF  K + +
Sbjct: 27  EPKSV--IFALKD-PGWCQAMQEELDALSRNKT-WILVPPPVNQNILGCKWVFKTKLHSD 86

Query: 93  NEVTRYKARLVAQGFSQRRGIDYEETYSHVVDVITLRYLISLTVCEKLDM 143
             + R KARLVA+GF Q  GI + ETYS VV   T+R +  L V ++L++
Sbjct: 87  GTLDRLKARLVAKGFHQEEGIYFVETYSPVVRTATIRTI--LNVAQQLEV 130

[Close]

BLAST of CSPI03G25060 vs. ExPASy TrEMBL
Match: A0A438J6Z0 (Retrovirus-related Pol polyprotein from transposon RE1 OS=Vitis vinifera OX=29760 GN=RE1_1305 PE=4 SV=1)

HSP 1 Score: 328.2 bits (840), Expect = 3.2e-86
Identity = 161/243 (66.26%), Postives = 195/243 (80.25%), Query Frame = 0


Query: 3   GKRWNRTDVVVDNIFVYNVAHNITHENEDSEPKSVEECCNRKDWPKWKEVIQAKLNSLSK 62
           G++W++ ++V++NIF + VA +I   +ED EP++VEEC +R DWPKWKE IQA+LNSL+K
Sbjct: 252 GEKWDQKNIVINNIFAFQVASDIIRNDEDPEPRNVEECRHRNDWPKWKEAIQAELNSLTK 311

Query: 63  REVCGPVVHTPKGVKPMGFKWVFVHKRNENNEVTRYKARLVAQGFSQRRGIDYEETYSHV 122
           REV GPVV TP+ VKP+G+KWVFV KRNENNE+ RYKARLVAQGFSQR GIDYEETYS V
Sbjct: 312 REVFGPVVQTPEDVKPVGYKWVFVRKRNENNEIIRYKARLVAQGFSQRPGIDYEETYSPV 371

Query: 123 VDVITLRYLISLTVCEKLDMHLMDVVTTYLYGFLENEIYMKIPEGFKILESYNSKSRELY 182
           +D IT R+LISL V E LDM LMDV+T YLYG ++N+IYMKIPEGFK+ ++ N+K R +Y
Sbjct: 372 MDAITFRFLISLAVSEGLDMRLMDVITAYLYGSMDNDIYMKIPEGFKLPDANNTKPRSMY 431

Query: 183 SIKLQRSLYGLKQSGRMWYNRLSEYLLKE-------------DVKRDIFRPQEDNEELLG 233
           SIKLQRSLYGLKQSGRMWYNRLSEYLLKE             DVK D FRP + +EELLG
Sbjct: 432 SIKLQRSLYGLKQSGRMWYNRLSEYLLKEGASFKFSNVVRSLDVKNDPFRPCKKDEELLG 491

[Close]

BLAST of CSPI03G25060 vs. ExPASy TrEMBL
Match: A0A438FCH2 (Copia protein OS=Vitis vinifera OX=29760 GN=GIP_75 PE=4 SV=1)

HSP 1 Score: 322.0 bits (824), Expect = 2.3e-84
Identity = 162/264 (61.36%), Postives = 195/264 (73.86%), Query Frame = 0


Query: 2   TGKRWNRTDVVVDNIFVYNVAHNITHENEDSEPKSVEECCNRKDWPKWKEVIQAKLNSLS 61
           TG++W+R ++V++NIF + VA +I   +ED EP++VEEC +R DWPKWKE IQA+LNSL+
Sbjct: 53  TGEKWDRNNIVINNIFAFQVASDIIRNDEDPEPRNVEECRHRNDWPKWKEAIQAELNSLT 112

Query: 62  KREVCGPVVHTPKGVKPMGFKWVFVHKRNENNEVTRYKARLVAQGFSQRRGIDYEETYSH 121
           KREV GPVV TP+ VKP+G+KWVFV KRNENNE+ RYKARLVAQGFSQR GIDYEETYS 
Sbjct: 113 KREVFGPVVQTPEDVKPVGYKWVFVRKRNENNEIIRYKARLVAQGFSQRPGIDYEETYSP 172

Query: 122 VVDVITLRYLISLTVCEKLDMHLMDVVTTYLYGFLENEIYMKIPEGFKILESYNSKSREL 181
           V+D IT R+LISL V E LDM  MDV+T YLYG ++N+IYMKIPEGFK+ ++ N+K R +
Sbjct: 173 VMDAITFRFLISLAVSEGLDMRPMDVITAYLYGSMDNDIYMKIPEGFKLPDANNTKPRSM 232

Query: 182 YSIKLQRSLYGLKQSGRMWYNRLSEYLLKE------------------------------ 233
           YSIKLQRSLYGLKQSG MWYNRLSEYLLKE                              
Sbjct: 233 YSIKLQRSLYGLKQSGHMWYNRLSEYLLKEGYVNNPICPCIFIKKSETGFAIIAVSPMVV 292

[Close]

BLAST of CSPI03G25060 vs. ExPASy TrEMBL
Match: A0A438BTB5 (Copia protein OS=Vitis vinifera OX=29760 GN=GIP_44 PE=4 SV=1)

HSP 1 Score: 320.1 bits (819), Expect = 8.7e-84
Identity = 162/271 (59.78%), Postives = 195/271 (71.96%), Query Frame = 0


Query: 3   GKRWNRTDVVVDNIFVYNVAHNITHENEDSEPKSVEECCNRKDWPKWKEVIQAKLNSLSK 62
           G++W+R ++V++NIF + VA +I   +ED EP++VEEC +R DWPKWKE IQ +LNSL+K
Sbjct: 72  GEKWDRNNIVINNIFAFQVASDIIRNDEDPEPRNVEECRHRNDWPKWKEAIQVELNSLTK 131

Query: 63  REVCGPVVHTPKGVKPMGFKWVFVHKRNENNEVTRYKARLVAQGFSQRRGIDYEETYSHV 122
           REV GPVV TP+ VKP+G+KWVFV KRNENNE+ RYKARLVAQGFSQR GIDYEETYS V
Sbjct: 132 REVFGPVVQTPEDVKPVGYKWVFVRKRNENNEIIRYKARLVAQGFSQRPGIDYEETYSPV 191

Query: 123 VDVITLRYLISLTVCEKLDMHLMDVVTTYLYGFLENEIYMKIPEGFKILESYNSKSRELY 182
           +D IT R+LISL V E LDM LMDV+T YLYG ++N+IYMKIPEGFK+ ++ N+K R +Y
Sbjct: 192 MDAITFRFLISLAVSEGLDMRLMDVITAYLYGSMDNDIYMKIPEGFKLPDANNTKPRSMY 251

Query: 183 SIKLQRSLYGLKQSGRMWYNRLSEYLLKE------------------------------- 233
           SIKLQRSLYGLKQSGRMWYNRLSEYLLKE                               
Sbjct: 252 SIKLQRSLYGLKQSGRMWYNRLSEYLLKEGSSIFQMEFLVHQSTYIKKVLKRFYMDKAHP 311

[Close]

BLAST of CSPI03G25060 vs. ExPASy TrEMBL
Match: A0A438HZK0 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Vitis vinifera OX=29760 GN=POLX_792 PE=4 SV=1)

HSP 1 Score: 317.8 bits (813), Expect = 4.3e-83
Identity = 150/210 (71.43%), Postives = 180/210 (85.71%), Query Frame = 0


Query: 2   TGKRWNRTDVVVDNIFVYNVAHNITHENEDSEPKSVEECCNRKDWPKWKEVIQAKLNSLS 61
           TG++W+R ++V++NIF + VA +I   +ED EP++VEEC +R DWPKWKE IQA+LNSL+
Sbjct: 599 TGEKWDRNNIVINNIFAFQVASDIIRNDEDPEPRNVEECRHRNDWPKWKEAIQAELNSLT 658

Query: 62  KREVCGPVVHTPKGVKPMGFKWVFVHKRNENNEVTRYKARLVAQGFSQRRGIDYEETYSH 121
           KREV GPVV TP+ VKP+G+KWVFV KRNENNE+ RYKARLVAQGFSQR GIDYEETYS 
Sbjct: 659 KREVFGPVVQTPEDVKPVGYKWVFVRKRNENNEIIRYKARLVAQGFSQRPGIDYEETYSP 718

Query: 122 VVDVITLRYLISLTVCEKLDMHLMDVVTTYLYGFLENEIYMKIPEGFKILESYNSKSREL 181
           V+D IT R+LISL V E LDM LMDV+T YLYG ++N+IYMKIPEGFK+ ++ N+K R +
Sbjct: 719 VMDAITFRFLISLAVSEGLDMRLMDVITAYLYGSMDNDIYMKIPEGFKLPDANNTKPRSM 778

Query: 182 YSIKLQRSLYGLKQSGRMWYNRLSEYLLKE 212
           YSIKLQRSLYGLKQSGRMWYNRLSEYLLKE
Sbjct: 779 YSIKLQRSLYGLKQSGRMWYNRLSEYLLKE 808

[Close]

BLAST of CSPI03G25060 vs. ExPASy TrEMBL
Match: A0A438ID00 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Vitis vinifera OX=29760 GN=POLX_1841 PE=4 SV=1)

HSP 1 Score: 317.8 bits (813), Expect = 4.3e-83
Identity = 149/210 (70.95%), Postives = 180/210 (85.71%), Query Frame = 0


Query: 2   TGKRWNRTDVVVDNIFVYNVAHNITHENEDSEPKSVEECCNRKDWPKWKEVIQAKLNSLS 61
           TG++W+R ++V++NIF + VA +I   +ED EP++VEEC +R DWPKWKE IQA+LNSL+
Sbjct: 764 TGEKWDRNNIVINNIFAFQVAFDIIRNDEDPEPRNVEECRHRNDWPKWKEAIQAELNSLT 823

Query: 62  KREVCGPVVHTPKGVKPMGFKWVFVHKRNENNEVTRYKARLVAQGFSQRRGIDYEETYSH 121
           KREV GPVV TP+ VKP+G+KWVFV KRNENNE+ RYKARLVAQGFSQR G+DYEETYS 
Sbjct: 824 KREVFGPVVQTPEDVKPVGYKWVFVRKRNENNEIIRYKARLVAQGFSQRPGVDYEETYSP 883

Query: 122 VVDVITLRYLISLTVCEKLDMHLMDVVTTYLYGFLENEIYMKIPEGFKILESYNSKSREL 181
           V+D IT R+LIS  V E LDM LMDV+TTYLYG ++N+IYMKIPEGFK+ ++ N+K R +
Sbjct: 884 VMDAITFRFLISFAVSEGLDMRLMDVITTYLYGSMDNDIYMKIPEGFKLPDANNTKPRSM 943

Query: 182 YSIKLQRSLYGLKQSGRMWYNRLSEYLLKE 212
           YSIKLQRSLYGLKQSGRMWYNRLSEYLLKE
Sbjct: 944 YSIKLQRSLYGLKQSGRMWYNRLSEYLLKE 973

[Close]

BLAST of CSPI03G25060 vs. NCBI nr
Match: RVX04725.1 (Retrovirus-related Pol polyprotein from transposon RE1 [Vitis vinifera])

HSP 1 Score: 328.2 bits (840), Expect = 6.6e-86
Identity = 161/243 (66.26%), Postives = 195/243 (80.25%), Query Frame = 0


Query: 3   GKRWNRTDVVVDNIFVYNVAHNITHENEDSEPKSVEECCNRKDWPKWKEVIQAKLNSLSK 62
           G++W++ ++V++NIF + VA +I   +ED EP++VEEC +R DWPKWKE IQA+LNSL+K
Sbjct: 252 GEKWDQKNIVINNIFAFQVASDIIRNDEDPEPRNVEECRHRNDWPKWKEAIQAELNSLTK 311

Query: 63  REVCGPVVHTPKGVKPMGFKWVFVHKRNENNEVTRYKARLVAQGFSQRRGIDYEETYSHV 122
           REV GPVV TP+ VKP+G+KWVFV KRNENNE+ RYKARLVAQGFSQR GIDYEETYS V
Sbjct: 312 REVFGPVVQTPEDVKPVGYKWVFVRKRNENNEIIRYKARLVAQGFSQRPGIDYEETYSPV 371

Query: 123 VDVITLRYLISLTVCEKLDMHLMDVVTTYLYGFLENEIYMKIPEGFKILESYNSKSRELY 182
           +D IT R+LISL V E LDM LMDV+T YLYG ++N+IYMKIPEGFK+ ++ N+K R +Y
Sbjct: 372 MDAITFRFLISLAVSEGLDMRLMDVITAYLYGSMDNDIYMKIPEGFKLPDANNTKPRSMY 431

Query: 183 SIKLQRSLYGLKQSGRMWYNRLSEYLLKE-------------DVKRDIFRPQEDNEELLG 233
           SIKLQRSLYGLKQSGRMWYNRLSEYLLKE             DVK D FRP + +EELLG
Sbjct: 432 SIKLQRSLYGLKQSGRMWYNRLSEYLLKEGASFKFSNVVRSLDVKNDPFRPCKKDEELLG 491

[Close]

BLAST of CSPI03G25060 vs. NCBI nr
Match: RVW57676.1 (Copia protein [Vitis vinifera])

HSP 1 Score: 322.0 bits (824), Expect = 4.7e-84
Identity = 162/264 (61.36%), Postives = 195/264 (73.86%), Query Frame = 0


Query: 2   TGKRWNRTDVVVDNIFVYNVAHNITHENEDSEPKSVEECCNRKDWPKWKEVIQAKLNSLS 61
           TG++W+R ++V++NIF + VA +I   +ED EP++VEEC +R DWPKWKE IQA+LNSL+
Sbjct: 53  TGEKWDRNNIVINNIFAFQVASDIIRNDEDPEPRNVEECRHRNDWPKWKEAIQAELNSLT 112

Query: 62  KREVCGPVVHTPKGVKPMGFKWVFVHKRNENNEVTRYKARLVAQGFSQRRGIDYEETYSH 121
           KREV GPVV TP+ VKP+G+KWVFV KRNENNE+ RYKARLVAQGFSQR GIDYEETYS 
Sbjct: 113 KREVFGPVVQTPEDVKPVGYKWVFVRKRNENNEIIRYKARLVAQGFSQRPGIDYEETYSP 172

Query: 122 VVDVITLRYLISLTVCEKLDMHLMDVVTTYLYGFLENEIYMKIPEGFKILESYNSKSREL 181
           V+D IT R+LISL V E LDM  MDV+T YLYG ++N+IYMKIPEGFK+ ++ N+K R +
Sbjct: 173 VMDAITFRFLISLAVSEGLDMRPMDVITAYLYGSMDNDIYMKIPEGFKLPDANNTKPRSM 232

Query: 182 YSIKLQRSLYGLKQSGRMWYNRLSEYLLKE------------------------------ 233
           YSIKLQRSLYGLKQSG MWYNRLSEYLLKE                              
Sbjct: 233 YSIKLQRSLYGLKQSGHMWYNRLSEYLLKEGYVNNPICPCIFIKKSETGFAIIAVSPMVV 292

[Close]

BLAST of CSPI03G25060 vs. NCBI nr
Match: RVW14192.1 (Copia protein [Vitis vinifera])

HSP 1 Score: 320.1 bits (819), Expect = 1.8e-83
Identity = 162/271 (59.78%), Postives = 195/271 (71.96%), Query Frame = 0


Query: 3   GKRWNRTDVVVDNIFVYNVAHNITHENEDSEPKSVEECCNRKDWPKWKEVIQAKLNSLSK 62
           G++W+R ++V++NIF + VA +I   +ED EP++VEEC +R DWPKWKE IQ +LNSL+K
Sbjct: 72  GEKWDRNNIVINNIFAFQVASDIIRNDEDPEPRNVEECRHRNDWPKWKEAIQVELNSLTK 131

Query: 63  REVCGPVVHTPKGVKPMGFKWVFVHKRNENNEVTRYKARLVAQGFSQRRGIDYEETYSHV 122
           REV GPVV TP+ VKP+G+KWVFV KRNENNE+ RYKARLVAQGFSQR GIDYEETYS V
Sbjct: 132 REVFGPVVQTPEDVKPVGYKWVFVRKRNENNEIIRYKARLVAQGFSQRPGIDYEETYSPV 191

Query: 123 VDVITLRYLISLTVCEKLDMHLMDVVTTYLYGFLENEIYMKIPEGFKILESYNSKSRELY 182
           +D IT R+LISL V E LDM LMDV+T YLYG ++N+IYMKIPEGFK+ ++ N+K R +Y
Sbjct: 192 MDAITFRFLISLAVSEGLDMRLMDVITAYLYGSMDNDIYMKIPEGFKLPDANNTKPRSMY 251

Query: 183 SIKLQRSLYGLKQSGRMWYNRLSEYLLKE------------------------------- 233
           SIKLQRSLYGLKQSGRMWYNRLSEYLLKE                               
Sbjct: 252 SIKLQRSLYGLKQSGRMWYNRLSEYLLKEGSSIFQMEFLVHQSTYIKKVLKRFYMDKAHP 311

[Close]

BLAST of CSPI03G25060 vs. NCBI nr
Match: RVW26456.1 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Vitis vinifera])

HSP 1 Score: 317.8 bits (813), Expect = 8.9e-83
Identity = 150/210 (71.43%), Postives = 180/210 (85.71%), Query Frame = 0


Query: 2   TGKRWNRTDVVVDNIFVYNVAHNITHENEDSEPKSVEECCNRKDWPKWKEVIQAKLNSLS 61
           TG++W+R ++V++NIF + VA +I   +ED EP++VEEC +R DWPKWKE IQA+LNSL+
Sbjct: 325 TGEKWDRNNIVINNIFAFQVASDIIRNDEDPEPRNVEECRHRNDWPKWKEAIQAELNSLT 384

Query: 62  KREVCGPVVHTPKGVKPMGFKWVFVHKRNENNEVTRYKARLVAQGFSQRRGIDYEETYSH 121
           KREV GPVV TP+ VKP+G+KWVFV KRNENNE+ RYKARLVAQGFSQR GIDYEETYS 
Sbjct: 385 KREVFGPVVQTPEDVKPVGYKWVFVRKRNENNEIIRYKARLVAQGFSQRPGIDYEETYSP 444

Query: 122 VVDVITLRYLISLTVCEKLDMHLMDVVTTYLYGFLENEIYMKIPEGFKILESYNSKSREL 181
           V+D IT R+LISL V E LDM LMDV+T YLYG ++N+IYMKIPEGFK+ ++ N+K R +
Sbjct: 445 VMDAITFRFLISLAVSEGLDMRLMDVITAYLYGSMDNDIYMKIPEGFKLPDANNTKPRSM 504

Query: 182 YSIKLQRSLYGLKQSGRMWYNRLSEYLLKE 212
           YSIKLQRSLYGLKQSGRMWYNRLSEYLLKE
Sbjct: 505 YSIKLQRSLYGLKQSGRMWYNRLSEYLLKE 534

[Close]

BLAST of CSPI03G25060 vs. NCBI nr
Match: RVW94544.1 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Vitis vinifera])

HSP 1 Score: 317.8 bits (813), Expect = 8.9e-83
Identity = 149/210 (70.95%), Postives = 180/210 (85.71%), Query Frame = 0


Query: 2   TGKRWNRTDVVVDNIFVYNVAHNITHENEDSEPKSVEECCNRKDWPKWKEVIQAKLNSLS 61
           TG++W+R ++V++NIF + VA +I   +ED EP++VEEC +R DWPKWKE IQA+LNSL+
Sbjct: 764 TGEKWDRNNIVINNIFAFQVAFDIIRNDEDPEPRNVEECRHRNDWPKWKEAIQAELNSLT 823

Query: 62  KREVCGPVVHTPKGVKPMGFKWVFVHKRNENNEVTRYKARLVAQGFSQRRGIDYEETYSH 121
           KREV GPVV TP+ VKP+G+KWVFV KRNENNE+ RYKARLVAQGFSQR G+DYEETYS 
Sbjct: 824 KREVFGPVVQTPEDVKPVGYKWVFVRKRNENNEIIRYKARLVAQGFSQRPGVDYEETYSP 883

Query: 122 VVDVITLRYLISLTVCEKLDMHLMDVVTTYLYGFLENEIYMKIPEGFKILESYNSKSREL 181
           V+D IT R+LIS  V E LDM LMDV+TTYLYG ++N+IYMKIPEGFK+ ++ N+K R +
Sbjct: 884 VMDAITFRFLISFAVSEGLDMRLMDVITTYLYGSMDNDIYMKIPEGFKLPDANNTKPRSM 943

Query: 182 YSIKLQRSLYGLKQSGRMWYNRLSEYLLKE 212
           YSIKLQRSLYGLKQSGRMWYNRLSEYLLKE
Sbjct: 944 YSIKLQRSLYGLKQSGRMWYNRLSEYLLKE 973

[Close]

BLAST of CSPI03G25060 vs. TAIR 10
Match: AT4G23160.1 (cysteine-rich RLK (RECEPTOR-like protein kinase) 8 )

HSP 1 Score: 102.4 bits (254), Expect = 5.5e-22
Identity = 49/137 (35.77%), Postives = 85/137 (62.04%), Query Frame = 0


Query: 73  PKGVKPMGFKWVFVHKRNENNEVTRYKARLVAQGFSQRRGIDYEETYSHVVDVITLRYLI 132
           P   KP+G KWV+  K N +  + RYKARLVA+G++Q+ GID+ ET+S V  + +++ ++
Sbjct: 121 PPNKKPIGCKWVYKIKYNSDGTIERYKARLVAKGYTQQEGIDFIETFSPVCKLTSVKLIL 180

Query: 133 SLTVCEKLDMHLMDVVTTYLYGFLENEIYMKIPEGFKILESYNSKSRELYSIKLQRSLYG 192
           +++      +H +D+   +L G L+ EIYMK+P G+   +  +     +  +K  +S+YG
Sbjct: 181 AISAIYNFTLHQLDISNAFLNGDLDEEIYMKLPPGYAARQGDSLPPNAVCYLK--KSIYG 240

Query: 193 LKQSGRMWYNRLSEYLL 210
           LKQ+ R W+ + S  L+
Sbjct: 241 LKQASRQWFLKFSVTLI 255

[Close]

BLAST of CSPI03G25060 vs. TAIR 10
Match: ATMG00820.1 (Reverse transcriptase (RNA-dependent DNA polymerase) )

HSP 1 Score: 64.3 bits (155), Expect = 1.7e-10
Identity = 44/110 (40.00%), Postives = 64/110 (58.18%), Query Frame = 0


Query: 33  EPKSVEECCNRKDWPKWKEVIQAKLNSLSKREVCGPVVHTPKGVKPMGFKWVFVHKRNEN 92
           EPKSV      KD P W + +Q +L++LS+ +    +V  P     +G KWVF  K + +
Sbjct: 27  EPKSV--IFALKD-PGWCQAMQEELDALSRNKT-WILVPPPVNQNILGCKWVFKTKLHSD 86

Query: 93  NEVTRYKARLVAQGFSQRRGIDYEETYSHVVDVITLRYLISLTVCEKLDM 143
             + R KARLVA+GF Q  GI + ETYS VV   T+R +  L V ++L++
Sbjct: 87  GTLDRLKARLVAKGFHQEEGIYFVETYSPVVRTATIRTI--LNVAQQLEV 130

The following BLAST results are available for this feature:

BLAST of CSPI03G25060 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P10978	1.7e-28	37.22	Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum...	[more]
P04146	6.7e-25	39.33	Copia protein OS=Drosophila melanogaster OX=7227 GN=GIP PE=1 SV=3	[more]
Q94HW2	9.7e-24	35.20	Retrovirus-related Pol polyprotein from transposon RE1 OS=Arabidopsis thaliana O...	[more]
Q9ZT94	1.7e-23	34.64	Retrovirus-related Pol polyprotein from transposon RE2 OS=Arabidopsis thaliana O...	[more]
P92520	2.3e-09	40.00	Uncharacterized mitochondrial protein AtMg00820 OS=Arabidopsis thaliana OX=3702 ...	[more]

BLAST of CSPI03G25060 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A438J6Z0	3.2e-86	66.26	Retrovirus-related Pol polyprotein from transposon RE1 OS=Vitis vinifera OX=2976...	[more]
A0A438FCH2	2.3e-84	61.36	Copia protein OS=Vitis vinifera OX=29760 GN=GIP_75 PE=4 SV=1	[more]
A0A438BTB5	8.7e-84	59.78	Copia protein OS=Vitis vinifera OX=29760 GN=GIP_44 PE=4 SV=1	[more]
A0A438HZK0	4.3e-83	71.43	Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Vitis vinifera OX...	[more]
A0A438ID00	4.3e-83	70.95	Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Vitis vinifera OX...	[more]

BLAST of CSPI03G25060 vs. NCBI nr


Match Name	E-value	Identity	Description
RVX04725.1	6.6e-86	66.26	Retrovirus-related Pol polyprotein from transposon RE1 [Vitis vinifera]	[more]
RVW57676.1	4.7e-84	61.36	Copia protein [Vitis vinifera]	[more]
RVW14192.1	1.8e-83	59.78	Copia protein [Vitis vinifera]	[more]
RVW26456.1	8.9e-83	71.43	Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Vitis vinifera]	[more]
RVW94544.1	8.9e-83	70.95	Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Vitis vinifera]	[more]

BLAST of CSPI03G25060 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G23160.1	5.5e-22	35.77	cysteine-rich RLK (RECEPTOR-like protein kinase) 8	[more]
ATMG00820.1	1.7e-10	40.00	Reverse transcriptase (RNA-dependent DNA polymerase)	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013103	Reverse transcriptase, RNA-dependent DNA polymerase	PFAM	PF07727	RVT_2	coord: 69..216 e-value: 3.9E-39 score: 134.7
None	No IPR available	PANTHER	PTHR45895	FAMILY NOT NAMED	coord: 14..219

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G25060.1	CSPI03G25060.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0015074	DNA integration
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0008270	zinc ion binding