CSPI03G24620 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI03G24620
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Reverse transcriptase domain-containing protein
Location	Chr3: 21711884 .. 21713827 (-)
RNA-Seq Expression	CSPI03G24620
Synteny	CSPI03G24620

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGCTTCGGATCGAGTCGAAGGTCACCACCCCAAGGATAGGGATTCTCTTCAGAGGCAAGCCACTGGCTCGACTCTTTCTTTCTCCCTTTCAGTTATTTAAGAGCTAAAAAAGCTCTTTCCCAACCAAAAAAGGCATAATGAACTACTAGAATACGACCCAAGCAAGCCAACCTATTGTCCACGAATGCAGTTCTTTCATCTCTTTCGGAATGAAAATAAGGAAGAGGAATTTAGTTACTCGATGCCCGTCATTCAAGGCGAGGCCAATGCTTGTCCCATCTGTAGCTTTTGAAGACACATAAGTTTTGTAGTTTAAGCAAAAGCTTAGTTCATTCACTCATTAATCTCAAAAATAATAAAAGATTCTCCATTCTCAATTCAATAAGGAATAAAAAGAAGAAAAGACGATAAAGGAGCTAGGTAATAAAAAGAAGAGAAGACGAGTCAAGAGGTTTTGATGATTGTAAAGACTATTACAAAGACCTGCAGAAACCTAAAAAGTACCAATGCTAAGAGAAAGAAGAGTATAGAATGAACGAGTCAATTCAATATGAACCTAGAAAAGTAGGCGTTTAAGATGAACCGAAAATCTTCAACAATATCCCTGGAGGGCATATCTGTGGAGAGGTAAGACGGAGGGTAGAGGTGGTGCTTAAGTTGCCTGGGATGAGGTTTGTCTTCCTTTTGATGAAGGAGGTCTTGCTATTCGCGATGGATCTTCTTGGAATATAGCAAGCACGTTAAAGATCTTATGGTTGCTTCTAGTTAAATCTGGTAGTTTGTGGGTTGCTTGGGTGGAAGCTTATATCCTTAAAGGGAGATCGCTCTTGGAGATCGATGTTGGGGTGGGTCGATCTTGGTGTTTTAGGGCAATCTTGCGTAAGCGGGATATCCTTAAAGCTCATGTTAAGATGGAGGTGGGCAATGGTAAGAAGTGTAGAGTGTGGTTGGATCCAAGGATTCAGGGTGGACCGATTATCCAGCAGTTTGGGGAGAGGGTGATTTATGATGCAGATAGTCGGCGGGATGCGAGGCTTGTGGATTTCATGGGTCGGGATGGAGATTGGAGGTGGTCGCTTGTTTCTTTGGATTTGATGGACATTTGGGATAGAATTCAGGGAGTGAGGCCGAGTCCGAGTGTTGAGGATATGTGGGTCCGGGTGCCAGGTAGTCATGAGAGTTTTTCGATCGTCAGTGCGTGCGAGGCTATTCGTCCTCATAGTAGTAGGGTTGGCTGGTCGGGTTTACTGTGGGGTGGGGGAAATATTCCTAAGCACTCCTTCTATACTTGGTTGGCCATCAAGGATAGGTTGGGTACTAGAGATAGATTAAGTCGGTGGGATAGTTCGATTCCTTTATCGTGTATTCTTTGTGGAGGGAACTATGAGTCTCGTGACCATTTGTTTTTTCCTTGTCCTTTTGGGTGGGAGATTTGGTCTAGAATCCTTTTGTTTATGTCATCTTCTCACAGGATACGGTATTGGGGGGTTGAGTTATCTTAGATTTGCAATTAGGGTATTGGGAAGGGTGTGAGGAGAAAATTGTGGCGCCTTCTCTGGTGTGCTACAATTTATTTCATTTGGCAGGAGCGAAATCTTCGTCTTCATGGAGGTGCTGTTTGGGAGCCTATGGTTATTTTCCAGCTCATTCGGTCGTGTATTAAAGTCGTGCTGCTTCTTGGTCCAATTGAGTTCATGGTCTTATTTAGTGTGCTTTTCTTTTGCATGTCCTCGGGCTGTGGGGTTTTTTTTCTCTTTTTTGTCTTTGTTGTCTTTCTCTTTTCTTCTTGTCCTTTTATTGTTTGATACACTAGTTGGTTTCTTTTGGTTTTGGTTCTTGTCCCCGAGATGTGGGGTTGTTTTGGGTACTTATGGGTTGTTTTGTCTAGTTACTTGTTCTATGAGTGTTGTTCGCTCTTGTGCCTTGACCTCAGGCTGTGA

mRNA sequence

ATGGCGCTTCGGATCGAGTCGAAGGTCACCACCCCAAGGATAGGGATTCTCTTCAGAGGCAAGCCACTGGCTCGACTCTTTCTTTCTCCCTTTCATTCTTTCATCTCTTTCGGAATGAAAATAAGGAAGAGGAATTTAGTTACTCGATGCCCGTCATTCAAGGCGAGGCCAATGCTTGTCCCATCTGTTTGTCTTCCTTTTGATGAAGGAGGTCTTGCTATTCGCGATGGATCTTCTTGGAATATAGCAAGCACGTTAAAGATCTTATGGTTGCTTCTAGTTAAATCTGGTAGTTTGTGGGTTGCTTGGGTGGAAGCTTATATCCTTAAAGGGAGATCGCTCTTGGAGATCGATGTTGGGGTGGGTCGATCTTGGTGTTTTAGGGCAATCTTGCGTAAGCGGGATATCCTTAAAGCTCATGTTAAGATGGAGGTGGGCAATGGTAAGAAGTGTAGAGTGTGGTTGGATCCAAGGATTCAGGGTGGACCGATTATCCAGCAGTTTGGGGAGAGGGTGATTTATGATGCAGATAGTCGGCGGGATGCGAGGCTTGTGGATTTCATGGGTCGGGATGGAGATTGGAGGTGGTCGCTTGTTTCTTTGGATTTGATGGACATTTGGGATAGAATTCAGGGAGTGAGGCCGAGTCCGAGTGTTGAGGATATGTGGGTCCGGGTGCCAGGTAGTCATGAGAGTTTTTCGATCGTCAGTGCGTGCGAGGCTATTCGTCCTCATAGTAGTAGGGTTGGCTGGTCGGGTTTACTGTGGGGTGGGGGAAATATTCCTAAGCACTCCTTCTATACTTGGTTGGCCATCAAGGATAGGTTGGGTACTAGAGATAGATTAAGTCGGTGGGATAGTTCGATTCCTTTATCGTGTATTCTTTGTGGAGGGAACTATGAGTCTCGTGACCATTTGTTTTTTCCTTGTCCTTTTGGGTGGGAGATTTGGTCTAGAATCCTTTTGTTTATGTCATCTTCTCACAGGATACGGTATTGGGGGGAGCGAAATCTTCGTCTTCATGGAGGTGCTGTTTGGGAGCCTATGGTTATTTTCCAGCTCATTCGGTCGTGTATTAAAGTCGTGCTGCTTCTTGGTCCAATTGAGTTCATGATGTGGGGTTGTTTTGGGTACTTATGGGTTGTTTTGTCTAGTTACTTGTTCTATGAGTGTTGTTCGCTCTTGTGCCTTGACCTCAGGCTGTGA

Coding sequence (CDS)

ATGGCGCTTCGGATCGAGTCGAAGGTCACCACCCCAAGGATAGGGATTCTCTTCAGAGGCAAGCCACTGGCTCGACTCTTTCTTTCTCCCTTTCATTCTTTCATCTCTTTCGGAATGAAAATAAGGAAGAGGAATTTAGTTACTCGATGCCCGTCATTCAAGGCGAGGCCAATGCTTGTCCCATCTGTTTGTCTTCCTTTTGATGAAGGAGGTCTTGCTATTCGCGATGGATCTTCTTGGAATATAGCAAGCACGTTAAAGATCTTATGGTTGCTTCTAGTTAAATCTGGTAGTTTGTGGGTTGCTTGGGTGGAAGCTTATATCCTTAAAGGGAGATCGCTCTTGGAGATCGATGTTGGGGTGGGTCGATCTTGGTGTTTTAGGGCAATCTTGCGTAAGCGGGATATCCTTAAAGCTCATGTTAAGATGGAGGTGGGCAATGGTAAGAAGTGTAGAGTGTGGTTGGATCCAAGGATTCAGGGTGGACCGATTATCCAGCAGTTTGGGGAGAGGGTGATTTATGATGCAGATAGTCGGCGGGATGCGAGGCTTGTGGATTTCATGGGTCGGGATGGAGATTGGAGGTGGTCGCTTGTTTCTTTGGATTTGATGGACATTTGGGATAGAATTCAGGGAGTGAGGCCGAGTCCGAGTGTTGAGGATATGTGGGTCCGGGTGCCAGGTAGTCATGAGAGTTTTTCGATCGTCAGTGCGTGCGAGGCTATTCGTCCTCATAGTAGTAGGGTTGGCTGGTCGGGTTTACTGTGGGGTGGGGGAAATATTCCTAAGCACTCCTTCTATACTTGGTTGGCCATCAAGGATAGGTTGGGTACTAGAGATAGATTAAGTCGGTGGGATAGTTCGATTCCTTTATCGTGTATTCTTTGTGGAGGGAACTATGAGTCTCGTGACCATTTGTTTTTTCCTTGTCCTTTTGGGTGGGAGATTTGGTCTAGAATCCTTTTGTTTATGTCATCTTCTCACAGGATACGGTATTGGGGGGAGCGAAATCTTCGTCTTCATGGAGGTGCTGTTTGGGAGCCTATGGTTATTTTCCAGCTCATTCGGTCGTGTATTAAAGTCGTGCTGCTTCTTGGTCCAATTGAGTTCATGATGTGGGGTTGTTTTGGGTACTTATGGGTTGTTTTGTCTAGTTACTTGTTCTATGAGTGTTGTTCGCTCTTGTGCCTTGACCTCAGGCTGTGA

Protein sequence

MALRIESKVTTPRIGILFRGKPLARLFLSPFHSFISFGMKIRKRNLVTRCPSFKARPMLVPSVCLPFDEGGLAIRDGSSWNIASTLKILWLLLVKSGSLWVAWVEAYILKGRSLLEIDVGVGRSWCFRAILRKRDILKAHVKMEVGNGKKCRVWLDPRIQGGPIIQQFGERVIYDADSRRDARLVDFMGRDGDWRWSLVSLDLMDIWDRIQGVRPSPSVEDMWVRVPGSHESFSIVSACEAIRPHSSRVGWSGLLWGGGNIPKHSFYTWLAIKDRLGTRDRLSRWDSSIPLSCILCGGNYESRDHLFFPCPFGWEIWSRILLFMSSSHRIRYWGERNLRLHGGAVWEPMVIFQLIRSCIKVVLLLGPIEFMMWGCFGYLWVVLSSYLFYECCSLLCLDLRL*

Homology

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BLAST of CSPI03G24620 vs. ExPASy TrEMBL
Match: A0A5A7TKU4 (Non-LTR retroelement reverse transcriptase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold477G00080 PE=4 SV=1)

HSP 1 Score: 396.4 bits (1017), Expect = 1.5e-106
Identity = 190/320 (59.38%), Postives = 223/320 (69.69%), Query Frame = 0


Query: 74   IRDGSSWNIASTLKILWLLLVKSGSLWVAWVEAYILKGRSLLEIDVGVGRSWCFRAILRK 133
            +RDG SWNI STLKILWLL   S SLWVAWVEAYILK +SL  +D GV RSWC RAILRK
Sbjct: 846  VRDGPSWNITSTLKILWLLTSLS-SLWVAWVEAYILKRKSLWAVDSGVCRSWCLRAILRK 905

Query: 134  RDILKAHVKMEVGNGKKCRVWLDPRIQGGPIIQQFGERVIYDADSRRDARLVDFMGRDGD 193
            RD LK HV +EVG+G  CRVWLDP +QG PI++Q GERV+YDA SRR+ARL +F+G DG+
Sbjct: 906  RDSLKYHVWIEVGDGSSCRVWLDPWLQGVPILEQVGERVLYDAASRREARLSEFIGPDGE 965

Query: 194  WRWSLVSLDLMDIWDRIQGVRPSPSVEDMWVRVPGSHESFSIVSACEAIRPHSSRVGWSG 253
            W+W  VS++L+D+WDR+Q VRP  SV D WV VPG    FSI SA + IRP   RV W G
Sbjct: 966  WQWPRVSIELIDLWDRVQAVRPCLSVRDRWVWVPGHQGGFSIASALDTIRPRGGRVRWVG 1025

Query: 254  LLWGGGNIPKHSFYTWLAIKDRLGTRDRLSRWDSSIPLSCILCGGNYESRDHLFFPCPFG 313
            LLWGGGN+PKHSF  WL IK++LGTRDRL RWDSS+P+S ILC G  ESRDHLFF CPFG
Sbjct: 1026 LLWGGGNVPKHSFCAWLTIKNKLGTRDRLHRWDSSVPMSFILCQGGVESRDHLFFSCPFG 1085

Query: 314  WEIWSRILLFMSSSHRIRYWG---------------------------------ERNLRL 361
             ++WSR+L  M+SSHRIRYWG                                 E N RL
Sbjct: 1086 RDVWSRVLQVMASSHRIRYWGVELSWICHQGIGISVRRKLWRVLLCATTYFIWKEWNHRL 1145

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BLAST of CSPI03G24620 vs. ExPASy TrEMBL
Match: A0A5A7UP65 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold43054G00070 PE=4 SV=1)

HSP 1 Score: 365.9 bits (938), Expect = 2.1e-97
Identity = 180/326 (55.21%), Postives = 210/326 (64.42%), Query Frame = 0


Query: 68   DEGGLAIRDGSSWNIASTLKILWLLLVKSGSLWVAWVEAYILKGRSLLEIDVGVGRSWCF 127
            +EGGL IRDG++W  ASTLKILWL+L  SGSLWVAWVEAY+LKGRSL ++D  VGRSWC 
Sbjct: 715  EEGGLGIRDGTAWKFASTLKILWLMLTNSGSLWVAWVEAYVLKGRSLWDVDSRVGRSWCL 774

Query: 128  RAILRKRDILKAHVKMEVGNGKKCRVWLDPRIQGGPIIQQFGERVIYDADSRRDARLVDF 187
            RAILRK++ LK HV+M+VGNG +CRVWLDP +Q G I++Q GERV+YDA SRR+A L +F
Sbjct: 775  RAILRKQEKLKQHVRMKVGNGNRCRVWLDPWLQRGAILEQVGERVLYDAASRREASLSNF 834

Query: 188  MGRDGDWRWSLVSLDLMDIWDRIQGVRPSPSVEDMWVRVPGSHESFSIVSACEAIRPHSS 247
            +G DG+W W                                    FSI SA EAIRP   
Sbjct: 835  IGPDGEWLW--------------------------------PRGGFSIASAWEAIRPRGG 894

Query: 248  RVGWSGLLWGGGNIPKHSFYTWLAIKDRLGTRDRLSRWDSSIPLSCILCGGNYESRDHLF 307
            RV W GLLWGGGNIPKHSF  WLAIKDRLGTRDR  RWDSS+PLSCILC G  ESRDHLF
Sbjct: 895  RVLWDGLLWGGGNIPKHSFCAWLAIKDRLGTRDRFHRWDSSVPLSCILCEGGMESRDHLF 954

Query: 308  FPCPFGWEIWSRILLFMSSSHRIRYWG--------------------------------- 361
            F CPFG ++WSR+L  M+SSHRI +WG                                 
Sbjct: 955  FSCPFGGDVWSRVLRIMASSHRIGHWGVELSWICHQGIRKGVRRKLWRVLWCATIYFIWN 1008

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BLAST of CSPI03G24620 vs. ExPASy TrEMBL
Match: A0A1S3BSI8 (uncharacterized protein LOC103493225 OS=Cucumis melo OX=3656 GN=LOC103493225 PE=4 SV=1)

HSP 1 Score: 365.2 bits (936), Expect = 3.6e-97
Identity = 191/367 (52.04%), Postives = 223/367 (60.76%), Query Frame = 0


Query: 42  RKRNLVTRCPSFKARPMLVPSV------------CLP---FDEGGLAIRDGSSWNIASTL 101
           R R+   R  SF  R  LV SV             LP    +EGGL IRDG++W  ASTL
Sbjct: 607 RIRSRSARVLSFAGRLQLVCSVLCSLQVYWAIVFVLPAYVHNEGGLGIRDGTAWKFASTL 666

Query: 102 KILWLLLVKSGSLWVAWVEAYILKGRSLLEIDVGVGRSWCFRAILRKRDILKAHVKMEVG 161
           KILWL+L  SGSLWVAWVEAY+LKGRSL ++D  VGRSWC RAILRK++ LK HV+M+VG
Sbjct: 667 KILWLMLTNSGSLWVAWVEAYVLKGRSLWDVDSRVGRSWCLRAILRKQEKLKQHVRMKVG 726

Query: 162 NGKKCRVWLDPRIQGGPIIQQFGERVIYDADSRRDARLVDFMGRDGDWRWSLVSLDLMDI 221
           NG +CRVWLDP +Q G I+++ GERV+YDA SRR+A L +F+G DG+W W          
Sbjct: 727 NGNRCRVWLDPWLQRGAILERVGERVLYDAASRREASLSNFIGPDGEWLW---------- 786

Query: 222 WDRIQGVRPSPSVEDMWVRVPGSHESFSIVSACEAIRPHSSRVGWSGLLWGGGNIPKHSF 281
                                     FSI SA EAIRP   RV W GLLWGGGNIPKHSF
Sbjct: 787 ----------------------PRGGFSIASAWEAIRPRGGRVLWDGLLWGGGNIPKHSF 846

Query: 282 YTWLAIKDRLGTRDRLSRWDSSIPLSCILCGGNYESRDHLFFPCPFGWEIWSRILLFMSS 341
             WLAIKDRLGTRDR  RWDSS+PLSCILC G  ESRDHLFF CPFG ++WSR+L  M+S
Sbjct: 847 CAWLAIKDRLGTRDRFHRWDSSVPLSCILCEGGMESRDHLFFSCPFGGDVWSRVLRIMAS 906

Query: 342 SHRIRYWG---------------------------------ERNLRLHGGAVWEPMVIFQ 361
           SHRI +WG                                 ERN RLHGG   +P+VIF 
Sbjct: 907 SHRIGHWGVELSWICHQGIRKGVRRKLWRVLWCATIYFIWNERNHRLHGGQAPDPIVIFH 941

[Close]

BLAST of CSPI03G24620 vs. ExPASy TrEMBL
Match: A0A5D3D7P6 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold416G00080 PE=4 SV=1)

HSP 1 Score: 364.4 bits (934), Expect = 6.2e-97
Identity = 179/326 (54.91%), Postives = 210/326 (64.42%), Query Frame = 0


Query: 68  DEGGLAIRDGSSWNIASTLKILWLLLVKSGSLWVAWVEAYILKGRSLLEIDVGVGRSWCF 127
           +EGGL IRDG++W  ASTLKILWL+L  SGSLWVAWVEAY+LKGRSL ++D  VGRSWC 
Sbjct: 696 EEGGLGIRDGTAWKFASTLKILWLMLTNSGSLWVAWVEAYVLKGRSLWDVDSRVGRSWCL 755

Query: 128 RAILRKRDILKAHVKMEVGNGKKCRVWLDPRIQGGPIIQQFGERVIYDADSRRDARLVDF 187
           RAILRK++ LK HV+M+VGNG +CRVWLDP +Q G I+++ GERV+YDA SRR+A L +F
Sbjct: 756 RAILRKQEKLKQHVRMKVGNGNRCRVWLDPWLQRGAILERVGERVLYDAASRREASLSNF 815

Query: 188 MGRDGDWRWSLVSLDLMDIWDRIQGVRPSPSVEDMWVRVPGSHESFSIVSACEAIRPHSS 247
           +G DG+W W                                    FSI SA EAIRP   
Sbjct: 816 IGPDGEWLW--------------------------------PRGGFSIASAWEAIRPRGG 875

Query: 248 RVGWSGLLWGGGNIPKHSFYTWLAIKDRLGTRDRLSRWDSSIPLSCILCGGNYESRDHLF 307
           RV W GLLWGGGNIPKHSF  WLAIKDRLGTRDR  RWDSS+PLSCILC G  ESRDHLF
Sbjct: 876 RVLWDGLLWGGGNIPKHSFCAWLAIKDRLGTRDRFHRWDSSVPLSCILCEGGMESRDHLF 935

Query: 308 FPCPFGWEIWSRILLFMSSSHRIRYWG--------------------------------- 361
           F CPFG ++WSR+L  M+SSHRI +WG                                 
Sbjct: 936 FSCPFGGDVWSRVLRIMASSHRIGHWGVELSWICHQGIRKGVRRKLWRVLWCATIYFIWN 989

[Close]

BLAST of CSPI03G24620 vs. ExPASy TrEMBL
Match: A0A5A7TZS0 (Reverse transcriptase domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold19358G00020 PE=4 SV=1)

HSP 1 Score: 358.6 bits (919), Expect = 3.4e-95
Identity = 181/331 (54.68%), Postives = 210/331 (63.44%), Query Frame = 0


Query: 63   VCLPFDEGGLAIRDGSSWNIASTLKILWLLLVKSGSLWVAWVEAYILKGRSLLEIDVGVG 122
            VCLPF+EGGL IRDG SWNIA+TLKI   LL   GSLWVAW+EAYILKG+SL ++D  VG
Sbjct: 813  VCLPFEEGGLGIRDGPSWNIANTLKI---LLTNLGSLWVAWMEAYILKGKSLWDVDSRVG 872

Query: 123  RSWCFRAILRKRDILKAHVKMEVGNGKKCRVWLDPRIQGGPIIQQFGERVIYDADSRRDA 182
            RSWC RAILRKR+ +K HV                           GERV+YDA SRR+A
Sbjct: 873  RSWCLRAILRKREKMKHHV---------------------------GERVLYDAASRREA 932

Query: 183  RLVDFMGRDGDWRWSLVSLDLMDIWDRIQGVRPSPSVEDMWVRVPGSHESFSIVSACEAI 242
            +L DF+  +G+W W  VSL+L+D+W+R+Q V P  SV D WV VPG    FSI SA EAI
Sbjct: 933  KLSDFIDPNGEWLWPRVSLELIDLWERVQEVSPCLSVSDSWVWVPGRQGGFSIASAWEAI 992

Query: 243  RPHSSRVGWSGLLWGGGNIPKHSFYTWLAIKDRLGTRDRLSRWDSSIPLSCILCGGNYES 302
             P   RV W GLLWGGGNIPKHSF  WLAIKDRL TRDRL RWDSSIPLSCILC G  ES
Sbjct: 993  CPRGGRVLWDGLLWGGGNIPKHSFCAWLAIKDRLDTRDRLHRWDSSIPLSCILCQGGVES 1052

Query: 303  RDHLFFPCPFGWEIWSRILLFMSSSHRIRYWG---------------------------- 361
            RDHLFF CPFG ++WSR+   M SSHRI +WG                            
Sbjct: 1053 RDHLFFSCPFGGDVWSRVFQIMESSHRIGHWGVELSWICHRGIGKGVRRKLWRVLWCATI 1112

[Close]

BLAST of CSPI03G24620 vs. NCBI nr
Match: XP_031737556.1 (uncharacterized protein LOC116402448 [Cucumis sativus])

HSP 1 Score: 511.5 bits (1316), Expect = 6.5e-141
Identity = 235/237 (99.16%), Postives = 235/237 (99.16%), Query Frame = 0


Query: 98  SLWVAWVEAYILKGRSLLEIDVGVGRSWCFRAILRKRDILKAHVKMEVGNGKKCRVWLDP 157
           SLWVAWVEAYILKGRSL EIDVGVGRSWCFRAILRKRDILKAHVKMEVGNGKKCRVWLDP
Sbjct: 8   SLWVAWVEAYILKGRSLWEIDVGVGRSWCFRAILRKRDILKAHVKMEVGNGKKCRVWLDP 67

Query: 158 RIQGGPIIQQFGERVIYDADSRRDARLVDFMGRDGDWRWSLVSLDLMDIWDRIQGVRPSP 217
           RIQGGPIIQQFGERVIYDADSRRDARLVDFMGRDGDWRWSLVSLDLMDIWDRIQGVRPSP
Sbjct: 68  RIQGGPIIQQFGERVIYDADSRRDARLVDFMGRDGDWRWSLVSLDLMDIWDRIQGVRPSP 127

Query: 218 SVEDMWVRVPGSHESFSIVSACEAIRPHSSRVGWSGLLWGGGNIPKHSFYTWLAIKDRLG 277
           SVEDMWVRVPGSHESFSIVSACEAIRPHSSRVGWSGLLWGGGNIPKHSFYTWLAIKDRLG
Sbjct: 128 SVEDMWVRVPGSHESFSIVSACEAIRPHSSRVGWSGLLWGGGNIPKHSFYTWLAIKDRLG 187

Query: 278 TRDRLSRWDSSIPLSCILCGGNYESRDHLFFPCPFGWEIWSRILLFMSSSHRIRYWG 335
           TRDRLSRWDSSIPLSCILCG NYESRDHLFFPCPFGWEIWSRILLFMSSSHRIRYWG
Sbjct: 188 TRDRLSRWDSSIPLSCILCGRNYESRDHLFFPCPFGWEIWSRILLFMSSSHRIRYWG 244

[Close]

BLAST of CSPI03G24620 vs. NCBI nr
Match: XP_031737043.1 (uncharacterized protein LOC116402131 [Cucumis sativus])

HSP 1 Score: 443.0 bits (1138), Expect = 2.8e-120
Identity = 228/324 (70.37%), Postives = 239/324 (73.77%), Query Frame = 0


Query: 63  VCLPFDEGGLAIRDGSSWNIASTLKILWLLLVKSGSLWVAWVEAYILKGRSLLE-IDVGV 122
           VCLPFDEGGLAIRDGSSWNIASTLKILWLLLVKSGSLWVAWVEAYILKGRS+L  +D+GV
Sbjct: 641 VCLPFDEGGLAIRDGSSWNIASTLKILWLLLVKSGSLWVAWVEAYILKGRSMLGWVDLGV 700

Query: 123 GRSWCFRAILRKRDILKAHVKMEVGNGKKCRVWLDPRIQGGPIIQQFGERVIYDADSRRD 182
               C   I  K   L    +  +    +C  W    IQGG IIQQFGERVIYDA SRRD
Sbjct: 701 LGLSCVSGISIK---LILRWRWAMAGSLECG-W----IQGGTIIQQFGERVIYDAGSRRD 760

Query: 183 ARLVDFMGRDGDWRWSLVSLDLMDIWDRIQGVRPSPSVEDMWVRVPGSHESFSIVSACEA 242
           ARLVDFM RDGDWRW LVSLDLMDIWD IQGVRPS SVED WV VPGS +SFSI SA E 
Sbjct: 761 ARLVDFMVRDGDWRWPLVSLDLMDIWDWIQGVRPSSSVEDRWVWVPGSLDSFSIASAWET 820

Query: 243 IRPHSSRVGWSGLLWGGGNIPKHSFYTWLAIKDRLGTRDRLSRWDSSIPLSCILCGGNYE 302
           IRPHSSRVGWSGLLW  GNIPKHSFY WLAI+DRLGTRDRLS+WD SIPLSC+LCGGNYE
Sbjct: 821 IRPHSSRVGWSGLLWDRGNIPKHSFYAWLAIRDRLGTRDRLSQWDRSIPLSCMLCGGNYE 880

Query: 303 SRDHLFFPCPFGWEIWSRILLFMSSSHRIRYWG--------------------------- 353
           SRDHLFF CPFGWEIWSRILLFMSSSHRI YWG                           
Sbjct: 881 SRDHLFFSCPFGWEIWSRILLFMSSSHRIGYWGVELSWICNQGIGKGVRRKLWHLLWCAT 940

[Close]

BLAST of CSPI03G24620 vs. NCBI nr
Match: XP_031745730.1 (uncharacterized protein LOC116406187 [Cucumis sativus])

HSP 1 Score: 408.3 bits (1048), Expect = 7.7e-110
Identity = 193/227 (85.02%), Postives = 201/227 (88.55%), Query Frame = 0


Query: 63  VCLPFDEGGLAIRDGSSWNIASTLKILWLLLVKSGSLWVAWVEAYILKGRSLLEIDVGVG 122
           VCLPFDEGGL IRDGSSWNIASTLKILWLLLVKSGSLWVAWVE+YILKGRSL EID GVG
Sbjct: 410 VCLPFDEGGLDIRDGSSWNIASTLKILWLLLVKSGSLWVAWVESYILKGRSLWEIDAGVG 469

Query: 123 RSWCFRAILRKRDILKAHVKMEVGNGKKCRVWLDPRIQGGPIIQQFGERVIYDADSRRDA 182
           RSWCFR ILRK+DILKAHVKMEVGNG+K RVWL P IQGGPIIQQFGERVIYDA SR DA
Sbjct: 470 RSWCFREILRKQDILKAHVKMEVGNGRKYRVWLVPWIQGGPIIQQFGERVIYDAGSRWDA 529

Query: 183 RLVDFMGRDGDWRWSLVSLDLMDIWDRIQGVRPSPSVEDMWVRVPGSHESFSIVSACEAI 242
           RL+DFMGRDGDWRW LV LDLMDIWDR+QGVRPSPSVED WV VPGSH+SFSI S  E I
Sbjct: 530 RLMDFMGRDGDWRWPLVYLDLMDIWDRVQGVRPSPSVEDRWVWVPGSHDSFSITSVWETI 589

Query: 243 RPHSSRVGWSGLLWGGGNIPKHSFYTWLAIKDRLGTRDRLSRWDSSI 290
           RPHSSRVGWSGLLWGGGNIPKHSF  WLAI+DRLGTR    R +  I
Sbjct: 590 RPHSSRVGWSGLLWGGGNIPKHSFCAWLAIRDRLGTRGNQERQERPI 636

[Close]

BLAST of CSPI03G24620 vs. NCBI nr
Match: KAA0042317.1 (non-LTR retroelement reverse transcriptase-like protein [Cucumis melo var. makuwa] >TYK15441.1 non-LTR retroelement reverse transcriptase-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 396.4 bits (1017), Expect = 3.0e-106
Identity = 190/320 (59.38%), Postives = 223/320 (69.69%), Query Frame = 0


Query: 74   IRDGSSWNIASTLKILWLLLVKSGSLWVAWVEAYILKGRSLLEIDVGVGRSWCFRAILRK 133
            +RDG SWNI STLKILWLL   S SLWVAWVEAYILK +SL  +D GV RSWC RAILRK
Sbjct: 846  VRDGPSWNITSTLKILWLLTSLS-SLWVAWVEAYILKRKSLWAVDSGVCRSWCLRAILRK 905

Query: 134  RDILKAHVKMEVGNGKKCRVWLDPRIQGGPIIQQFGERVIYDADSRRDARLVDFMGRDGD 193
            RD LK HV +EVG+G  CRVWLDP +QG PI++Q GERV+YDA SRR+ARL +F+G DG+
Sbjct: 906  RDSLKYHVWIEVGDGSSCRVWLDPWLQGVPILEQVGERVLYDAASRREARLSEFIGPDGE 965

Query: 194  WRWSLVSLDLMDIWDRIQGVRPSPSVEDMWVRVPGSHESFSIVSACEAIRPHSSRVGWSG 253
            W+W  VS++L+D+WDR+Q VRP  SV D WV VPG    FSI SA + IRP   RV W G
Sbjct: 966  WQWPRVSIELIDLWDRVQAVRPCLSVRDRWVWVPGHQGGFSIASALDTIRPRGGRVRWVG 1025

Query: 254  LLWGGGNIPKHSFYTWLAIKDRLGTRDRLSRWDSSIPLSCILCGGNYESRDHLFFPCPFG 313
            LLWGGGN+PKHSF  WL IK++LGTRDRL RWDSS+P+S ILC G  ESRDHLFF CPFG
Sbjct: 1026 LLWGGGNVPKHSFCAWLTIKNKLGTRDRLHRWDSSVPMSFILCQGGVESRDHLFFSCPFG 1085

Query: 314  WEIWSRILLFMSSSHRIRYWG---------------------------------ERNLRL 361
             ++WSR+L  M+SSHRIRYWG                                 E N RL
Sbjct: 1086 RDVWSRVLQVMASSHRIRYWGVELSWICHQGIGISVRRKLWRVLLCATTYFIWKEWNHRL 1145

[Close]

BLAST of CSPI03G24620 vs. NCBI nr
Match: XP_031745548.1 (uncharacterized protein LOC116405965 [Cucumis sativus])

HSP 1 Score: 374.0 bits (959), Expect = 1.6e-99
Identity = 179/241 (74.27%), Postives = 186/241 (77.18%), Query Frame = 0


Query: 153 VWLDPRIQGGPIIQQFGERVIYDADSRRDARLVDFMGRDGDWRWSLVSLDLMDIWDRIQG 212
           VWL P IQGGPIIQQFG+RVIYD  SR DARLVDFMGRDGDWRW LVSLDLMDIWDR+QG
Sbjct: 5   VWLVPWIQGGPIIQQFGKRVIYDVGSRWDARLVDFMGRDGDWRWPLVSLDLMDIWDRVQG 64

Query: 213 VRPSPSVEDMWVRVPGSHESFSIVSACEAIRPHSSRVGWSGLLWGGGNIPKHSFYTWLAI 272
           VRPSPSVED WV V GSH+SFSI SA E IRPHS RVGWSGLLWGGGNIPKHSF  WLAI
Sbjct: 65  VRPSPSVEDRWVWVSGSHDSFSITSAWETIRPHSIRVGWSGLLWGGGNIPKHSFCAWLAI 124

Query: 273 KDRLGTRDRLSRWDSSIPLSCILCGGNYESRDHLFFPCPFGWEIWSRILLFMSSSHRIRY 332
           +DRLGTRDRLSRWD SIPLSC+LCGGNYESRDHLFF C FGWEIWSRILL MSSSHRI Y
Sbjct: 125 RDRLGTRDRLSRWDRSIPLSCLLCGGNYESRDHLFFSCHFGWEIWSRILLLMSSSHRIGY 184

Query: 333 WG---------------------------------ERNLRLHGGAVWEPMVIFQLIRSCI 361
           WG                                 ERN RLHGGA+ EPMV+FQLIRSCI
Sbjct: 185 WGVGLSWIYNQGIGKSVRRKLWRLLWCATIYFIWQERNHRLHGGAIREPMVVFQLIRSCI 244

[Close]

BLAST of CSPI03G24620 vs. TAIR 10
Match: AT1G60720.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 120.6 bits (301), Expect = 3.0e-27
Identity = 86/274 (31.39%), Postives = 129/274 (47.08%), Query Frame = 0


Query: 130 ILRKRDILKAHVKMEVGNGKKCRVWLDPRIQGGPIIQQFGERVIYDADSRR---DARLVD 189
           +L  R + +  VK  +GNG+    W D     GP+I+  G+   Y + S R   +AR+V+
Sbjct: 2   LLFLRPLAEQFVKCNLGNGRIAHFWHDSWTSLGPLIKVMGD---YGSRSLRIPLNARVVE 61

Query: 190 FMGRDGDWRWSLV-SLDLMDIWDRIQGV-RPSP-SVEDMWVRVPGS--HESFSIVSACEA 249
            +G +G W+  L  S     I D I  +  PSP ++ED +  V G    + FS     +A
Sbjct: 62  ALGVNG-WKLPLSRSAPAQAIHDHISTITTPSPATIEDSFDWVVGGVVCQGFSSARTWDA 121

Query: 250 IRPHSSRVGWSGLLWGGGNIPKHSFYTWLAIKDRLGTRDRLSRWDSSIPLSCILCGGNYE 309
           IRP +  + W+  +W  G +PKH+F  W++  DRL TR RL+ W       C LC    E
Sbjct: 122 IRPRAPELDWAKAVWFKGAVPKHAFNMWISQLDRLPTRQRLASWGHIQSFDCCLCTIETE 181

Query: 310 SRDHLFFPCPFGWEIW----SRI--------------------------LLFMSSSHRIR 364
           SRDHL F C F  ++W    SR+                          LL   S+H I 
Sbjct: 182 SRDHLLFSCEFAAQVWRLAFSRLCPRQRLFCSWAELLSWMRSSSSSAPSLLRKVSAHAII 241

[Close]

BLAST of CSPI03G24620 vs. TAIR 10
Match: AT5G16486.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 114.0 bits (284), Expect = 2.8e-25
Identity = 71/205 (34.63%), Postives = 99/205 (48.29%), Query Frame = 0


Query: 124 SWCFRAILRKRDILKAHVKMEVGNGKKCRVWLDPRIQGGPIIQQFGERVIYDADSRRDAR 183
           SW +++I + R + +  V  +VG+G  C  W +     GP+I   G+     +   R+A 
Sbjct: 8   SWIWKSICKLRPMAREFVVCKVGSGITCNFWSENWTNLGPLIHLTGDLGPRVSGLPRNAS 67

Query: 184 LVDFMGRDGDWRWSLVSLDLMDIWDRIQGVRPSPSV-------ED---MWVRVPGSHESF 243
           + D + RDG W W   S     I   ++   P  SV       ED   MW +V GS  S 
Sbjct: 68  VADAL-RDGVW-WINGSRSRNPIIQLLKNCLPLSSVVNLQVDAEDDLFMW-KVGGSEASV 127

Query: 244 SIVSACEAI--RPHSSRVGWSGLLWGGGNIPKHSFYTWLAIKDRLGTRDRLSRWDSSIPL 303
              SA   I   P   +V W   +W  G IPKH+F +W+ I+ RL TRD+L  W   +P 
Sbjct: 128 GFSSAATWIHLNPVGEKVDWHKAIWFKGRIPKHAFISWVNIRHRLPTRDKLLSWGLHVPS 187

Query: 304 SCILCGGNYESRDHLFFPCPFGWEI 317
            C+LC    E+R HLFF C F  EI
Sbjct: 188 LCLLCNAFDETRQHLFFDCVFAGEI 209

[Close]

BLAST of CSPI03G24620 vs. TAIR 10
Match: AT3G24255.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 111.7 bits (278), Expect = 1.4e-24
Identity = 98/340 (28.82%), Postives = 138/340 (40.59%), Query Frame = 0


Query: 25  RLFLSPFHSFISFGMKIRK------RNLVTRCPSF---------KARPMLVPSVCLPFDE 84
           +L  S  HS  +F M   +      + + + C SF         K   +    VC P DE
Sbjct: 68  QLISSVIHSLTNFWMSAFRLPSACIKEIDSICSSFLWSGPELNTKKAKVAWSDVCTPKDE 127

Query: 85  GGLAIRDGSSWNIASTLKILWLLLVKSGSLWVAWVEAYILKGRSLLEIDVGVGRSWCFRA 144
           GGL IR               L     GS W        + G + L        SW ++ 
Sbjct: 128 GGLGIRS--------------LKEANKGSFW-------SISGNTTL-------GSWMWKK 187

Query: 145 ILRKRDILKAHVKMEVGNGKKCRVWLDPRIQGGPIIQQFGERVIYDADSRRDARLVDFMG 204
           IL+ R +    VK ++ NG     W D   + G +I   G R   D      A + + + 
Sbjct: 188 ILKHRALASGFVKHDIHNGSNTSFWFDNWSKIGRLIDVTGHRGCIDMGITLHASVAEAVV 247

Query: 205 RDGDWRWSLVSLDLMDIWDRIQGVRPS--PSVEDMWVRVPGSHE----SFSIVSACEAIR 264
                R    +  L+ I D I  VR     S ED  VR  G+ +     F+      A R
Sbjct: 248 NHRPRRHRHDT--LLRIEDVIAEVRHQGLTSGEDT-VRWKGNGDIFKPCFNTKETWAATR 307

Query: 265 PHSSRVGWSGLLWGGGNIPKHSFYTWLAIKDRLGTRDRLSRWDSSIPLSCILCGGNYESR 324
               +V W   +W     PK+S   W+AIK+RL T DR+  W++    SC+LC    E+R
Sbjct: 308 EPKLKVNWYKGVWFSHATPKYSVLAWIAIKNRLTTGDRMLSWNAGADSSCVLCHHLVETR 367

Query: 325 DHLFFPCPFGWEI-WSRILLFMSSSHRIRYWGERNLRLHG 343
           DHLFF CP+  E+ +     F  + H +  W ERN R HG
Sbjct: 368 DHLFFTCPYSAEVPFLTRYTFQLTLHSL--WKERNGRRHG 374

[Close]

BLAST of CSPI03G24620 vs. TAIR 10
Match: AT4G04650.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 99.4 bits (246), Expect = 7.1e-21
Identity = 57/191 (29.84%), Postives = 85/191 (44.50%), Query Frame = 0


Query: 134 RDILKAHVKMEVGNGKKCRVWLDPRIQGGPIIQQFGERVIYDADSRRDARLVDFMGRDGD 193
           R + +  +  EVG+G   + W D  I  GP+I+  G           DA + D +     
Sbjct: 5   RVVARPFIVCEVGSGVTAKFWHDNWIGLGPLIEVIGPLGPRTVGLPIDAVVRDALRGTSW 64

Query: 194 WRWSL-----VSLDLMDIWDRIQGVRPSPSVED-MW-VRVPGSHESFSIVSACEAIRPHS 253
           W  S      + + L ++    QG+      +  +W   +      FS      A+ P S
Sbjct: 65  WIASSRSRNPIIVQLKNLLPEAQGLLDCQHDDSFLWKTDLHAPSNRFSAPRTWSALHPQS 124

Query: 254 SRVGWSGLLWGGGNIPKHSFYTWLAIKDRLGTRDRLSRWDSSIPLSCILCGGNYESRDHL 313
             V W   +W   ++PKH+F  W+   +RL TRDRL  W  SIP  C+LC  + +SR HL
Sbjct: 125 HTVPWHKAVWFKNHVPKHAFICWVVAWNRLHTRDRLQNWGLSIPAECLLCNAHDDSRAHL 184

Query: 314 FFPCPFGWEIW 318
           FF C F   +W
Sbjct: 185 FFECQFSGVVW 195

[Close]

BLAST of CSPI03G24620 vs. TAIR 10
Match: AT1G43730.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 99.0 bits (245), Expect = 9.3e-21
Identity = 59/194 (30.41%), Postives = 83/194 (42.78%), Query Frame = 0


Query: 124 SWCFRAILRKRDILKAHVKMEVGNGKKCRVWLDPRIQGGPIIQQFGERVIYDADSRRDAR 183
           SW +R + + R++ +  V  +VG+G   + W D     GP+I   G           DA 
Sbjct: 50  SWIWRRLCKLREVARPFVVCDVGSGVTAKFWHDNWTGHGPLIDLVGPLGPQTVGLPIDAV 109

Query: 184 LVDFMGRDGDWRWSLVSLDLMDIWDRIQGVRPSPSVEDMWVRVPGSHESFSIVSACEAIR 243
            +     D  + W     DL            +PS              FS      A+ 
Sbjct: 110 GLIDCQHDDSFIW---KTDL-----------HAPS------------NIFSTAKTSLALH 169

Query: 244 PHSSRVGWSGLLWGGGNIPKHSFYTWLAIKDRLGTRDRLSRWDSSIPLSCILCGGNYESR 303
           P +  V W   +W   ++PKH+F  W+   +RL TRDRL  W  SIP  C+LC  + ESR
Sbjct: 170 PQNHIVPWYKAVWFKNHVPKHAFICWVVAWNRLHTRDRLRSWGLSIPAVCLLCNSHDESR 217

Query: 304 DHLFFPCPFGWEIW 318
            HLFF CPF   +W
Sbjct: 230 AHLFFECPFCGAVW 217

The following BLAST results are available for this feature:

BLAST of CSPI03G24620 vs.


Match Name	E-value	Identity	Description

BLAST of CSPI03G24620 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7TKU4	1.5e-106	59.38	Non-LTR retroelement reverse transcriptase-like protein OS=Cucumis melo var. mak...	[more]
A0A5A7UP65	2.1e-97	55.21	Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold43...	[more]
A0A1S3BSI8	3.6e-97	52.04	uncharacterized protein LOC103493225 OS=Cucumis melo OX=3656 GN=LOC103493225 PE=...	[more]
A0A5D3D7P6	6.2e-97	54.91	Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold41...	[more]
A0A5A7TZS0	3.4e-95	54.68	Reverse transcriptase domain-containing protein OS=Cucumis melo var. makuwa OX=1...	[more]

BLAST of CSPI03G24620 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_031737556.1	6.5e-141	99.16	uncharacterized protein LOC116402448 [Cucumis sativus]	[more]
XP_031737043.1	2.8e-120	70.37	uncharacterized protein LOC116402131 [Cucumis sativus]	[more]
XP_031745730.1	7.7e-110	85.02	uncharacterized protein LOC116406187 [Cucumis sativus]	[more]
KAA0042317.1	3.0e-106	59.38	non-LTR retroelement reverse transcriptase-like protein [Cucumis melo var. makuw...	[more]
XP_031745548.1	1.6e-99	74.27	uncharacterized protein LOC116405965 [Cucumis sativus]	[more]

BLAST of CSPI03G24620 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G60720.1	3.0e-27	31.39	RNA-directed DNA polymerase (reverse transcriptase)-related family protein	[more]
AT5G16486.1	2.8e-25	34.63	RNA-directed DNA polymerase (reverse transcriptase)-related family protein	[more]
AT3G24255.1	1.4e-24	28.82	RNA-directed DNA polymerase (reverse transcriptase)-related family protein	[more]
AT4G04650.1	7.1e-21	29.84	RNA-directed DNA polymerase (reverse transcriptase)-related family protein	[more]
AT1G43730.1	9.3e-21	30.41	RNA-directed DNA polymerase (reverse transcriptase)-related family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR026960	Reverse transcriptase zinc-binding domain	PFAM	PF13966	zf-RVT	coord: 233..317 e-value: 5.7E-19 score: 68.6
None	No IPR available	PANTHER	PTHR33116:SF40	BNAA09G28300D PROTEIN	coord: 63..157 coord: 245..321
None	No IPR available	PANTHER	PTHR33116	REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATED	coord: 245..321
None	No IPR available	PANTHER	PTHR33116	REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATED	coord: 63..157

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G24620.1	CSPI03G24620.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016020	membrane