CSPI03G24610 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI03G24610
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	rRNA intron-encoded homing endonuclease
Location	Chr3: 21709492 .. 21709820 (+)
RNA-Seq Expression	CSPI03G24610
Synteny	CSPI03G24610

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATTAACAAGAACGGTCGGGGGCATTCGTATTTCATAGTCAGAGGTGAAACTCTTGGATTTATGAAAGACGAACAACTGCGAAAGCATTTGCCAAGGATGTTTTCATTAATCAAGAACGAAAGTTGGGGGCTCGAAGACGATCAGATACCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATTGGCGGATGTTGCTTTAAGGACTCCGCCAGCACCTTATGAGAAATCAAAGTCTTTGGGTTCCGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAA

mRNA sequence

ATGATTAACAAGAACGGTCGGGGGCATTCACGAACAACTGCGAAAGCATTTGCCAAGGATGTTTTCATTAATCAAGAACGAAAGTTGGGGGCTCGAAGACGATCAGATACCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATTGGCGGATGTTGCTTTAAGGACTCCGCCAGCACCTTATGAGAAATCAAAGTCTTTGGGTTCCGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAA

Coding sequence (CDS)

ATGATTAACAAGAACGGTCGGGGGCATTCACGAACAACTGCGAAAGCATTTGCCAAGGATGTTTTCATTAATCAAGAACGAAAGTTGGGGGCTCGAAGACGATCAGATACCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATTGGCGGATGTTGCTTTAAGGACTCCGCCAGCACCTTATGAGAAATCAAAGTCTTTGGGTTCCGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAA

Protein sequence

MINKNGRGHSRTTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGLADVALRTPPAPYEKSKSLGSGGSMVARLKLKGIDGRAPPGVEPAA*

Homology

[Close]

BLAST of CSPI03G24610 vs. ExPASy TrEMBL
Match: A0A5B6TKV7 (rRNA intron-encoded homing endonuclease OS=Gossypium australe OX=47621 GN=EPI10_034356 PE=4 SV=1)

HSP 1 Score: 179.1 bits (453), Expect = 8.8e-42
Identity = 90/95 (94.74%), Postives = 92/95 (96.84%), Query Frame = 0


Query: 1  MINKNGRGHSRTTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGLADVALRTPPA 60
          MIN++ RGHSRTTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQG ADVA RTPPA
Sbjct: 1  MINRDSRGHSRTTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVAFRTPPA 60

Query: 61 PYEKSKSLGSGGSMVARLKLKGIDGRAPPGVEPAA 96
          PYEKSKSLGSGGSMVARLKLKGIDGRAPPGVEPAA
Sbjct: 61 PYEKSKSLGSGGSMVARLKLKGIDGRAPPGVEPAA 95

[Close]

BLAST of CSPI03G24610 vs. ExPASy TrEMBL
Match: A0A4S8JRQ3 (Uncharacterized protein OS=Musa balbisiana OX=52838 GN=C4D60_Mb01t29820 PE=4 SV=1)

HSP 1 Score: 178.3 bits (451), Expect = 1.5e-41
Identity = 90/95 (94.74%), Postives = 92/95 (96.84%), Query Frame = 0


Query: 1  MINKNGRGHSRTTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGLADVALRTPPA 60
          MI ++ RGHSRTTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQG ADVALRTPPA
Sbjct: 1  MIKRDSRGHSRTTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVALRTPPA 60

Query: 61 PYEKSKSLGSGGSMVARLKLKGIDGRAPPGVEPAA 96
          PYEKSKSLGSGGSMVARLKLKGIDGRAPPGVEPAA
Sbjct: 61 PYEKSKSLGSGGSMVARLKLKGIDGRAPPGVEPAA 95

[Close]

BLAST of CSPI03G24610 vs. ExPASy TrEMBL
Match: A0A6A5P3K8 (Uncharacterized protein OS=Lupinus albus OX=3870 GN=Lal_00031671 PE=4 SV=1)

HSP 1 Score: 175.3 bits (443), Expect = 1.3e-40
Identity = 89/95 (93.68%), Postives = 91/95 (95.79%), Query Frame = 0


Query: 1  MINKNGRGHSRTTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGLADVALRTPPA 60
          MIN++ RGHSRTTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQG ADVA RTP A
Sbjct: 1  MINRDSRGHSRTTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVAFRTPLA 60

Query: 61 PYEKSKSLGSGGSMVARLKLKGIDGRAPPGVEPAA 96
          PYEKSKSLGSGGSMVARLKLKGIDGRAPPGVEPAA
Sbjct: 61 PYEKSKSLGSGGSMVARLKLKGIDGRAPPGVEPAA 95

[Close]

BLAST of CSPI03G24610 vs. ExPASy TrEMBL
Match: A0A072TTZ2 (rRNA intron-encoded homing endonuclease, putative OS=Medicago truncatula OX=3880 GN=MTR_0052s0110 PE=4 SV=1)

HSP 1 Score: 175.3 bits (443), Expect = 1.3e-40
Identity = 89/95 (93.68%), Postives = 91/95 (95.79%), Query Frame = 0


Query: 1   MINKNGRGHSRTTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGLADVALRTPPA 60
           MIN++ RGHSRTTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQG ADVA RTP A
Sbjct: 246 MINRDSRGHSRTTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVAFRTPLA 305

Query: 61  PYEKSKSLGSGGSMVARLKLKGIDGRAPPGVEPAA 96
           PYEKSKSLGSGGSMVARLKLKGIDGRAPPGVEPAA
Sbjct: 306 PYEKSKSLGSGGSMVARLKLKGIDGRAPPGVEPAA 340

[Close]

BLAST of CSPI03G24610 vs. ExPASy TrEMBL
Match: A0A6A1VJU8 (Uncharacterized protein OS=Morella rubra OX=262757 GN=CJ030_MR5G025804 PE=4 SV=1)

HSP 1 Score: 172.9 bits (437), Expect = 6.3e-40
Identity = 92/109 (84.40%), Postives = 93/109 (85.32%), Query Frame = 0


Query: 1   MINKNGRGHS--------------RTTAKAFAKDVFINQERKLGARRRSDTVLVSTINDA 60
           MIN+N RGHS              RTTAKAFAKDVFINQERKLGARRRSDTVLVSTINDA
Sbjct: 1   MINRNSRGHSYFIVRGEISWIYERRTTAKAFAKDVFINQERKLGARRRSDTVLVSTINDA 60

Query: 61  DQGLADVALRTPPAPYEKSKSLGSGGSMVARLKLKGIDGRAPPGVEPAA 96
           DQG ADVALRTPPAPYEKSKSLGSGGSMVARLKLKGIDGRAPPGVEPAA
Sbjct: 61  DQGSADVALRTPPAPYEKSKSLGSGGSMVARLKLKGIDGRAPPGVEPAA 109

[Close]

BLAST of CSPI03G24610 vs. NCBI nr
Match: KAA3441672.1 (rRNA intron-encoded homing endonuclease [Gossypium australe])

HSP 1 Score: 179.1 bits (453), Expect = 1.8e-41
Identity = 90/95 (94.74%), Postives = 92/95 (96.84%), Query Frame = 0


Query: 1  MINKNGRGHSRTTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGLADVALRTPPA 60
          MIN++ RGHSRTTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQG ADVA RTPPA
Sbjct: 1  MINRDSRGHSRTTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVAFRTPPA 60

Query: 61 PYEKSKSLGSGGSMVARLKLKGIDGRAPPGVEPAA 96
          PYEKSKSLGSGGSMVARLKLKGIDGRAPPGVEPAA
Sbjct: 61 PYEKSKSLGSGGSMVARLKLKGIDGRAPPGVEPAA 95

[Close]

BLAST of CSPI03G24610 vs. NCBI nr
Match: THU64758.1 (hypothetical protein C4D60_Mb01t29820 [Musa balbisiana] >THU64761.1 hypothetical protein C4D60_Mb01t29850 [Musa balbisiana])

HSP 1 Score: 178.3 bits (451), Expect = 3.1e-41
Identity = 90/95 (94.74%), Postives = 92/95 (96.84%), Query Frame = 0


Query: 1  MINKNGRGHSRTTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGLADVALRTPPA 60
          MI ++ RGHSRTTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQG ADVALRTPPA
Sbjct: 1  MIKRDSRGHSRTTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVALRTPPA 60

Query: 61 PYEKSKSLGSGGSMVARLKLKGIDGRAPPGVEPAA 96
          PYEKSKSLGSGGSMVARLKLKGIDGRAPPGVEPAA
Sbjct: 61 PYEKSKSLGSGGSMVARLKLKGIDGRAPPGVEPAA 95

[Close]

BLAST of CSPI03G24610 vs. NCBI nr
Match: KEH17025.1 (rRNA intron-encoded homing endonuclease, putative [Medicago truncatula])

HSP 1 Score: 175.3 bits (443), Expect = 2.6e-40
Identity = 89/95 (93.68%), Postives = 91/95 (95.79%), Query Frame = 0


Query: 1   MINKNGRGHSRTTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGLADVALRTPPA 60
           MIN++ RGHSRTTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQG ADVA RTP A
Sbjct: 246 MINRDSRGHSRTTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVAFRTPLA 305

Query: 61  PYEKSKSLGSGGSMVARLKLKGIDGRAPPGVEPAA 96
           PYEKSKSLGSGGSMVARLKLKGIDGRAPPGVEPAA
Sbjct: 306 PYEKSKSLGSGGSMVARLKLKGIDGRAPPGVEPAA 340

[Close]

BLAST of CSPI03G24610 vs. NCBI nr
Match: KAF1854471.1 (hypothetical protein Lal_00046860 [Lupinus albus] >TKY45570.1 rRNA intron-encoded homing endonuclease [Spatholobus suberectus] >KAF1855499.1 hypothetical protein Lal_00043751 [Lupinus albus] >KAF1856636.1 hypothetical protein Lal_00043455 [Lupinus albus] >KAF1856638.1 hypothetical protein Lal_00043457 [Lupinus albus])

HSP 1 Score: 175.3 bits (443), Expect = 2.6e-40
Identity = 89/95 (93.68%), Postives = 91/95 (95.79%), Query Frame = 0


Query: 1  MINKNGRGHSRTTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGLADVALRTPPA 60
          MIN++ RGHSRTTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQG ADVA RTP A
Sbjct: 1  MINRDSRGHSRTTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVAFRTPLA 60

Query: 61 PYEKSKSLGSGGSMVARLKLKGIDGRAPPGVEPAA 96
          PYEKSKSLGSGGSMVARLKLKGIDGRAPPGVEPAA
Sbjct: 61 PYEKSKSLGSGGSMVARLKLKGIDGRAPPGVEPAA 95

[Close]

BLAST of CSPI03G24610 vs. NCBI nr
Match: KAB1212993.1 (hypothetical protein CJ030_MR5G025804 [Morella rubra])

HSP 1 Score: 172.9 bits (437), Expect = 1.3e-39
Identity = 92/109 (84.40%), Postives = 93/109 (85.32%), Query Frame = 0


Query: 1   MINKNGRGHS--------------RTTAKAFAKDVFINQERKLGARRRSDTVLVSTINDA 60
           MIN+N RGHS              RTTAKAFAKDVFINQERKLGARRRSDTVLVSTINDA
Sbjct: 1   MINRNSRGHSYFIVRGEISWIYERRTTAKAFAKDVFINQERKLGARRRSDTVLVSTINDA 60

Query: 61  DQGLADVALRTPPAPYEKSKSLGSGGSMVARLKLKGIDGRAPPGVEPAA 96
           DQG ADVALRTPPAPYEKSKSLGSGGSMVARLKLKGIDGRAPPGVEPAA
Sbjct: 61  DQGSADVALRTPPAPYEKSKSLGSGGSMVARLKLKGIDGRAPPGVEPAA 109

The following BLAST results are available for this feature:

BLAST of CSPI03G24610 vs.


Match Name	E-value	Identity	Description

BLAST of CSPI03G24610 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5B6TKV7	8.8e-42	94.74	rRNA intron-encoded homing endonuclease OS=Gossypium australe OX=47621 GN=EPI10_...	[more]
A0A4S8JRQ3	1.5e-41	94.74	Uncharacterized protein OS=Musa balbisiana OX=52838 GN=C4D60_Mb01t29820 PE=4 SV=...	[more]
A0A6A5P3K8	1.3e-40	93.68	Uncharacterized protein OS=Lupinus albus OX=3870 GN=Lal_00031671 PE=4 SV=1	[more]
A0A072TTZ2	1.3e-40	93.68	rRNA intron-encoded homing endonuclease, putative OS=Medicago truncatula OX=3880...	[more]
A0A6A1VJU8	6.3e-40	84.40	Uncharacterized protein OS=Morella rubra OX=262757 GN=CJ030_MR5G025804 PE=4 SV=1	[more]

BLAST of CSPI03G24610 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA3441672.1	1.8e-41	94.74	rRNA intron-encoded homing endonuclease [Gossypium australe]	[more]
THU64758.1	3.1e-41	94.74	hypothetical protein C4D60_Mb01t29820 [Musa balbisiana] >THU64761.1 hypothetical...	[more]
KEH17025.1	2.6e-40	93.68	rRNA intron-encoded homing endonuclease, putative [Medicago truncatula]	[more]
KAF1854471.1	2.6e-40	93.68	hypothetical protein Lal_00046860 [Lupinus albus] >TKY45570.1 rRNA intron-encode...	[more]
KAB1212993.1	1.3e-39	84.40	hypothetical protein CJ030_MR5G025804 [Morella rubra]	[more]

BLAST of CSPI03G24610 vs. NCBI nr


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR34410	INTRON-ENCODED HOMING ENDONUCLEASE, PUTATIVE-RELATED	coord: 1..95

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G24610.1	CSPI03G24610.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0015074	DNA integration
biological_process	GO:0090305	nucleic acid phosphodiester bond hydrolysis
biological_process	GO:0006508	proteolysis
biological_process	GO:0006278	RNA-dependent DNA biosynthetic process
molecular_function	GO:0004190	aspartic-type endopeptidase activity
molecular_function	GO:0004519	endonuclease activity
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0003964	RNA-directed DNA polymerase activity