CSPI03G24370 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI03G24370
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionF-box domain-containing protein
LocationChr3: 21452070 .. 21453721 (-)
RNA-Seq ExpressionCSPI03G24370
SyntenyCSPI03G24370
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GTGGCAAATTGGTTCCATGCAATAGCACACATAACTCCCACGTGTCACCAATAAAATCCCCCGCTCTATAAATAATCAAATTAATCCAATCCTCACCTAACCGATATCTTTCCCAGCGCTTCTTCATGGCACAACCTCTTCCCTCACTTTCTTCTTCACCACCGCCGTGGGAGGTGATTCTTCTCGTCGCCGAACGGCTCGACCCCAAAACCCTGGCCACCGCCTCCTGCGTTTGCAAGTCTTGGTCCCTTTCCATGGCCTCCGACCACCTTTGGGAGCCTATTTTCACCGCTAATTTCCCTTCTCTCTTTAACCTTGCAACCGCCCCGGCCACGTCACCACACGTGTCCTTCCGCCGCCTCTTTGGCCTCGGCTACATTGCTGCCGCTCGCCGCCGTCCCGCTCAGTCGAAACCTTCTCTTTCCCTCTCCGACCTCGTCTTTGTTATTAGTATTTCCTTCACCAACAAAGATAGGTGTAGTATTGCTTGTGACGGAGTGAATAAAGAAGAAAAAGGTAATCTTTAGTGCTTTTAACACATTGAGAAGCTACGTTATATATAACATAGGGTCCGTTGGGTTGATTCTAAGACTTGGTAACCTTTATTTTTTTCTTTTAACTTAAGTAAAAATTATACAAAAAATCTAAAGATAGTTTTTAATTTAATTTTTTAAAAAGCAAACATCATCGATGCATCTCACCAACAATTTTCAATTATAACGTTGACACGTCTCGTACTGGGTGTGATGTCGCATGCACACATGTATTGCCCATTTTTTATTTCGGTTTGAGTGGGTCTACTCCTTATCTCAACCTGAACTAAACACTTTAGCAAAAACGATCGAACTGGTAAAATGATTATTTTCTTTCCTTTTTCTTTTTCTTTTTCTTTTTTTTTTTTTTTTGCCATTTTTGCTTAATTGGTAAGTAAATGGGCATTTTGCTGTATACATGAAAAGAACTTTAGGAGTTATTATTTGCAAAATTTCACAGGTTTATAAAAAGTTAAGGGTAGTAAAAGACCTAAAGGCTAAAACATTCTCTCTCTTTTCCCGATAAAATCAATCCAAAAGTTTGGAAAGTAATAAACGTAACTTTTATTTTTATTTACTTATTTTGCTTATTACTAAAAAATTCTAGCTAGAATTCGAATGTATATATAATTCTTTATTACATGAAAACTTGTAGCTCCGAGGCCACAATCCAGCCTCACCATCGTGAAGCATGGCGAGGAGCTACCCGTAGATCGAAATGGACTATTCAAATTCGACATAAATCTCAACGAGAATGGGGACGGTGCTCCGGTGGTCATTGGAGCGAGAGAGGAGGTGGAGGTGGTATGGAACATAGTGTTAGAAGGGTGGAGGGGGGTATTTACAATGATTGAATGTGGAGGAAGAGTGGGAATGGCTCCTAGAGCGGATGGGTGGTTCTCGGAGGAGCTTCCGGCAGCGGGGTGCTGCCCGAACGTCGTGAGTGGCGGCATTGTGGGGGATTTGAGACTAGGGTTGTGTGGAAATGGAGGAGGGAGTAATAAGGTTAGGATTGAAAGTGTGAGTGTAGGGATGATGAGTGTTGTGAGTTGGAGATATGTGAGCTTAGATGATGGGTTAATGTATTTGCAACATTTTTTGTTCAATGCTAACGTCTAG

mRNA sequence

GTGGCAAATTGGTTCCATGCAATAGCACACATAACTCCCACGTGTCACCAATAAAATCCCCCGCTCTATAAATAATCAAATTAATCCAATCCTCACCTAACCGATATCTTTCCCAGCGCTTCTTCATGGCACAACCTCTTCCCTCACTTTCTTCTTCACCACCGCCGTGGGAGGTGATTCTTCTCGTCGCCGAACGGCTCGACCCCAAAACCCTGGCCACCGCCTCCTGCGTTTGCAAGTCTTGGTCCCTTTCCATGGCCTCCGACCACCTTTGGGAGCCTATTTTCACCGCTAATTTCCCTTCTCTCTTTAACCTTGCAACCGCCCCGGCCACGTCACCACACGTGTCCTTCCGCCGCCTCTTTGGCCTCGGCTACATTGCTGCCGCTCGCCGCCGTCCCGCTCAGTCGAAACCTTCTCTTTCCCTCTCCGACCTCGTCTTTGTTATTAGTATTTCCTTCACCAACAAAGATAGGTGTAGTATTGCTTGTGACGGAGTGAATAAAGAAGAAAAAGCTCCGAGGCCACAATCCAGCCTCACCATCGTGAAGCATGGCGAGGAGCTACCCGTAGATCGAAATGGACTATTCAAATTCGACATAAATCTCAACGAGAATGGGGACGGTGCTCCGGTGGTCATTGGAGCGAGAGAGGAGGTGGAGGTGGTATGGAACATAGTGTTAGAAGGGTGGAGGGGGGTATTTACAATGATTGAATGTGGAGGAAGAGTGGGAATGGCTCCTAGAGCGGATGGGTGGTTCTCGGAGGAGCTTCCGGCAGCGGGGTGCTGCCCGAACGTCGTGAGTGGCGGCATTGTGGGGGATTTGAGACTAGGGTTGTGTGGAAATGGAGGAGGGAGTAATAAGGTTAGGATTGAAAGTGTGAGTGTAGGGATGATGAGTGTTGTGAGTTGGAGATATGTGAGCTTAGATGATGGGTTAATGTATTTGCAACATTTTTTGTTCAATGCTAACGTCTAG

Coding sequence (CDS)

ATGGCACAACCTCTTCCCTCACTTTCTTCTTCACCACCGCCGTGGGAGGTGATTCTTCTCGTCGCCGAACGGCTCGACCCCAAAACCCTGGCCACCGCCTCCTGCGTTTGCAAGTCTTGGTCCCTTTCCATGGCCTCCGACCACCTTTGGGAGCCTATTTTCACCGCTAATTTCCCTTCTCTCTTTAACCTTGCAACCGCCCCGGCCACGTCACCACACGTGTCCTTCCGCCGCCTCTTTGGCCTCGGCTACATTGCTGCCGCTCGCCGCCGTCCCGCTCAGTCGAAACCTTCTCTTTCCCTCTCCGACCTCGTCTTTGTTATTAGTATTTCCTTCACCAACAAAGATAGGTGTAGTATTGCTTGTGACGGAGTGAATAAAGAAGAAAAAGCTCCGAGGCCACAATCCAGCCTCACCATCGTGAAGCATGGCGAGGAGCTACCCGTAGATCGAAATGGACTATTCAAATTCGACATAAATCTCAACGAGAATGGGGACGGTGCTCCGGTGGTCATTGGAGCGAGAGAGGAGGTGGAGGTGGTATGGAACATAGTGTTAGAAGGGTGGAGGGGGGTATTTACAATGATTGAATGTGGAGGAAGAGTGGGAATGGCTCCTAGAGCGGATGGGTGGTTCTCGGAGGAGCTTCCGGCAGCGGGGTGCTGCCCGAACGTCGTGAGTGGCGGCATTGTGGGGGATTTGAGACTAGGGTTGTGTGGAAATGGAGGAGGGAGTAATAAGGTTAGGATTGAAAGTGTGAGTGTAGGGATGATGAGTGTTGTGAGTTGGAGATATGTGAGCTTAGATGATGGGTTAATGTATTTGCAACATTTTTTGTTCAATGCTAACGTCTAG

Protein sequence

MAQPLPSLSSSPPPWEVILLVAERLDPKTLATASCVCKSWSLSMASDHLWEPIFTANFPSLFNLATAPATSPHVSFRRLFGLGYIAAARRRPAQSKPSLSLSDLVFVISISFTNKDRCSIACDGVNKEEKAPRPQSSLTIVKHGEELPVDRNGLFKFDINLNENGDGAPVVIGAREEVEVVWNIVLEGWRGVFTMIECGGRVGMAPRADGWFSEELPAAGCCPNVVSGGIVGDLRLGLCGNGGGSNKVRIESVSVGMMSVVSWRYVSLDDGLMYLQHFLFNANV*
Homology
BLAST of CSPI03G24370 vs. ExPASy Swiss-Prot
Match: Q5EAF6 (Probable F-box protein At5g04010 OS=Arabidopsis thaliana OX=3702 GN=NSFBx PE=2 SV=1)

HSP 1 Score: 109.8 bits (273), Expect = 5.3e-23
Identity = 78/272 (28.68%), Postives = 130/272 (47.79%), Query Frame = 0

Query: 11  SPPPWEVILLVAERLDPKTLATASCVCKSWSLSMASDHLWEPIFTANFPSLFNLATAPAT 70
           SPP WE++ LV   +DP++LA ASCV  +WS   +S+ LW+ +            T    
Sbjct: 51  SPPSWEILCLVGPYMDPESLAVASCVSTTWSKCFSSEDLWKSLPATRHSIFAKAITKEGA 110

Query: 71  SP-HVSFRRLFGLGYIAAARRRPAQ-SKPSLSLSDLVFVISISFTNKDRCSIACDGVNKE 130
            P  +S++RL      AA RRR  Q ++P +SLSDLVF++ +S  +K+            
Sbjct: 111 KPAWLSYKRLISEAESAAKRRRNNQPAEPKISLSDLVFIVHVSAGSKE------------ 170

Query: 131 EKAPRPQSSLTIVKHGEELPVDRNGLFKFDINLNENGDGAPVVIGAREEVEVVWNIVLEG 190
                      +VK G++L    N  F+ + ++ ++G  A +     ++V + WN+VL  
Sbjct: 171 ---------AVVVKQGKDLVFGSNERFQIEADVRDSGFTADM-----KDVSMSWNVVLRN 230

Query: 191 WRGVFTMIECGGRVGMAPRADGWFSEELPAAGCCPNVVSG-GIVGDLRLGLCGNGGGSNK 250
           +  +F M E    +       GWF++ELP  G       G  +VG+++          N+
Sbjct: 231 YERMFLMSET--VIKSLDSMIGWFTDELP--GTKNRYCDGSNLVGEVKPSF-------NE 285

Query: 251 VRIESVSVGMMSVVSWRYVSLDDGLMYLQHFL 280
             ++ V   +    +W+ + +DD L YLQ FL
Sbjct: 291 DVLDKVVFAIADSRNWKSLFVDDVLRYLQCFL 285

BLAST of CSPI03G24370 vs. ExPASy TrEMBL
Match: A0A0A0LDE5 (F-box domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G497390 PE=4 SV=1)

HSP 1 Score: 580.1 bits (1494), Expect = 5.1e-162
Identity = 284/284 (100.00%), Postives = 284/284 (100.00%), Query Frame = 0

Query: 1   MAQPLPSLSSSPPPWEVILLVAERLDPKTLATASCVCKSWSLSMASDHLWEPIFTANFPS 60
           MAQPLPSLSSSPPPWEVILLVAERLDPKTLATASCVCKSWSLSMASDHLWEPIFTANFPS
Sbjct: 1   MAQPLPSLSSSPPPWEVILLVAERLDPKTLATASCVCKSWSLSMASDHLWEPIFTANFPS 60

Query: 61  LFNLATAPATSPHVSFRRLFGLGYIAAARRRPAQSKPSLSLSDLVFVISISFTNKDRCSI 120
           LFNLATAPATSPHVSFRRLFGLGYIAAARRRPAQSKPSLSLSDLVFVISISFTNKDRCSI
Sbjct: 61  LFNLATAPATSPHVSFRRLFGLGYIAAARRRPAQSKPSLSLSDLVFVISISFTNKDRCSI 120

Query: 121 ACDGVNKEEKAPRPQSSLTIVKHGEELPVDRNGLFKFDINLNENGDGAPVVIGAREEVEV 180
           ACDGVNKEEKAPRPQSSLTIVKHGEELPVDRNGLFKFDINLNENGDGAPVVIGAREEVEV
Sbjct: 121 ACDGVNKEEKAPRPQSSLTIVKHGEELPVDRNGLFKFDINLNENGDGAPVVIGAREEVEV 180

Query: 181 VWNIVLEGWRGVFTMIECGGRVGMAPRADGWFSEELPAAGCCPNVVSGGIVGDLRLGLCG 240
           VWNIVLEGWRGVFTMIECGGRVGMAPRADGWFSEELPAAGCCPNVVSGGIVGDLRLGLCG
Sbjct: 181 VWNIVLEGWRGVFTMIECGGRVGMAPRADGWFSEELPAAGCCPNVVSGGIVGDLRLGLCG 240

Query: 241 NGGGSNKVRIESVSVGMMSVVSWRYVSLDDGLMYLQHFLFNANV 285
           NGGGSNKVRIESVSVGMMSVVSWRYVSLDDGLMYLQHFLFNANV
Sbjct: 241 NGGGSNKVRIESVSVGMMSVVSWRYVSLDDGLMYLQHFLFNANV 284

BLAST of CSPI03G24370 vs. ExPASy TrEMBL
Match: A0A5D3DEJ6 (Putative F-box protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1721G00070 PE=4 SV=1)

HSP 1 Score: 545.8 bits (1405), Expect = 1.1e-151
Identity = 269/284 (94.72%), Postives = 276/284 (97.18%), Query Frame = 0

Query: 1   MAQPLPSLSSSPPPWEVILLVAERLDPKTLATASCVCKSWSLSMASDHLWEPIFTANFPS 60
           MAQPLPSLSSSPPPWEVILLVAERLDPKTLATASCVCKSWSLSMASDHLWEPIFTANFPS
Sbjct: 1   MAQPLPSLSSSPPPWEVILLVAERLDPKTLATASCVCKSWSLSMASDHLWEPIFTANFPS 60

Query: 61  LFNLATAPATSPHVSFRRLFGLGYIAAARRRPAQSKPSLSLSDLVFVISISFTNKDRCSI 120
           LFNLA+AP +SP VSFRRLFGLGYIAAARRRPAQ KP+LSLSDLVFVISISFT+KD+  I
Sbjct: 61  LFNLASAPTSSPPVSFRRLFGLGYIAAARRRPAQLKPTLSLSDLVFVISISFTDKDQSII 120

Query: 121 ACDGVNKEEKAPRPQSSLTIVKHGEELPVDRNGLFKFDINLNENGDGAPVVIGAREEVEV 180
           ACDGVNKEEKAPRPQSSLT+VKHGEELPV+RNGLFKFDINLNENG  APVVIGAREEVEV
Sbjct: 121 ACDGVNKEEKAPRPQSSLTVVKHGEELPVERNGLFKFDINLNENG-AAPVVIGAREEVEV 180

Query: 181 VWNIVLEGWRGVFTMIECGGRVGMAPRADGWFSEELPAAGCCPNVVSGGIVGDLRLGLCG 240
           VWNIVLEGWRGVFTMIECGGRVGMAPRADGWFSEELPAAGCCPN VSGGIVGDLRLGLCG
Sbjct: 181 VWNIVLEGWRGVFTMIECGGRVGMAPRADGWFSEELPAAGCCPNAVSGGIVGDLRLGLCG 240

Query: 241 NGGGSNKVRIESVSVGMMSVVSWRYVSLDDGLMYLQHFLFNANV 285
           NGGGSNKVRIESVSVGMMSVVSWRYVSLDDGLMYLQHFLFNANV
Sbjct: 241 NGGGSNKVRIESVSVGMMSVVSWRYVSLDDGLMYLQHFLFNANV 283

BLAST of CSPI03G24370 vs. ExPASy TrEMBL
Match: A0A1S3BLJ1 (probable F-box protein At5g04010 OS=Cucumis melo OX=3656 GN=LOC103491161 PE=4 SV=1)

HSP 1 Score: 545.8 bits (1405), Expect = 1.1e-151
Identity = 269/284 (94.72%), Postives = 276/284 (97.18%), Query Frame = 0

Query: 1   MAQPLPSLSSSPPPWEVILLVAERLDPKTLATASCVCKSWSLSMASDHLWEPIFTANFPS 60
           MAQPLPSLSSSPPPWEVILLVAERLDPKTLATASCVCKSWSLSMASDHLWEPIFTANFPS
Sbjct: 1   MAQPLPSLSSSPPPWEVILLVAERLDPKTLATASCVCKSWSLSMASDHLWEPIFTANFPS 60

Query: 61  LFNLATAPATSPHVSFRRLFGLGYIAAARRRPAQSKPSLSLSDLVFVISISFTNKDRCSI 120
           LFNLA+AP +SP VSFRRLFGLGYIAAARRRPAQ KP+LSLSDLVFVISISFT+KD+  I
Sbjct: 61  LFNLASAPTSSPPVSFRRLFGLGYIAAARRRPAQLKPTLSLSDLVFVISISFTDKDQSII 120

Query: 121 ACDGVNKEEKAPRPQSSLTIVKHGEELPVDRNGLFKFDINLNENGDGAPVVIGAREEVEV 180
           ACDGVNKEEKAPRPQSSLT+VKHGEELPV+RNGLFKFDINLNENG  APVVIGAREEVEV
Sbjct: 121 ACDGVNKEEKAPRPQSSLTVVKHGEELPVERNGLFKFDINLNENG-AAPVVIGAREEVEV 180

Query: 181 VWNIVLEGWRGVFTMIECGGRVGMAPRADGWFSEELPAAGCCPNVVSGGIVGDLRLGLCG 240
           VWNIVLEGWRGVFTMIECGGRVGMAPRADGWFSEELPAAGCCPN VSGGIVGDLRLGLCG
Sbjct: 181 VWNIVLEGWRGVFTMIECGGRVGMAPRADGWFSEELPAAGCCPNAVSGGIVGDLRLGLCG 240

Query: 241 NGGGSNKVRIESVSVGMMSVVSWRYVSLDDGLMYLQHFLFNANV 285
           NGGGSNKVRIESVSVGMMSVVSWRYVSLDDGLMYLQHFLFNANV
Sbjct: 241 NGGGSNKVRIESVSVGMMSVVSWRYVSLDDGLMYLQHFLFNANV 283

BLAST of CSPI03G24370 vs. ExPASy TrEMBL
Match: A0A6J1EEG3 (probable F-box protein At5g04010 OS=Cucurbita moschata OX=3662 GN=LOC111432489 PE=4 SV=1)

HSP 1 Score: 410.2 bits (1053), Expect = 6.9e-111
Identity = 217/284 (76.41%), Postives = 237/284 (83.45%), Query Frame = 0

Query: 1   MAQPLPSLSSSPPPWEVILLVAERLDPKTLATASCVCKSWSLSMASDHLWEPIFTANFPS 60
           MAQ L     SPPPWEV+LLVAERLDP+TLATASCVCKSWS+SMAS++LWEPIF+A FPS
Sbjct: 1   MAQSLQP-PPSPPPWEVLLLVAERLDPRTLATASCVCKSWSISMASNNLWEPIFSAKFPS 60

Query: 61  LFNLATAPA-TSPHVSFRRLFGLGYIAAARRRPAQSKPSLSLSDLVFVISISFTNKDRCS 120
           L NLA + A TSP VSFRRLFGLG+ AAARRRPA SKP LSLSDLVFVIS+S T+K    
Sbjct: 61  LTNLAASSATTSPPVSFRRLFGLGHTAAARRRPAPSKPILSLSDLVFVISVSGTDKANGC 120

Query: 121 IACDGVNKEEKAPRPQSSLTIVKHGEELPVDRNGLFKFDINLNENGDGAPVVIGAREEVE 180
           I   G  KEEK   P+   T+VKHG EL VDRN LFKFDIN++ NGD   VVIGA EEVE
Sbjct: 121 IVSGGTKKEEK-DVPEPMFTVVKHGGELAVDRNRLFKFDINVS-NGDEV-VVIGAGEEVE 180

Query: 181 VVWNIVLEGWRGVFTMIECGGRVGMAPRADGWFSEELPAAGCCPNVVSGGIVGDLRLGLC 240
           VVWN+VLEGWR VFTMIECGGRVGMAPRADGWFSEELPAAGCCP  VSGGIV DL+LG+C
Sbjct: 181 VVWNVVLEGWRAVFTMIECGGRVGMAPRADGWFSEELPAAGCCPKEVSGGIVADLKLGVC 240

Query: 241 --GNGGGSNKVRIESVSVGMMSVVSWRYVSLDDGLMYLQHFLFN 282
             G GG ++KVRIESVSVGMMSV+SWRYVSLDDGLMYLQHFLFN
Sbjct: 241 KNGEGGKNDKVRIESVSVGMMSVLSWRYVSLDDGLMYLQHFLFN 280

BLAST of CSPI03G24370 vs. ExPASy TrEMBL
Match: A0A6J1HMX9 (probable F-box protein At5g04010 OS=Cucurbita maxima OX=3661 GN=LOC111466077 PE=4 SV=1)

HSP 1 Score: 404.8 bits (1039), Expect = 2.9e-109
Identity = 215/284 (75.70%), Postives = 237/284 (83.45%), Query Frame = 0

Query: 1   MAQPLPSLSSSPPPWEVILLVAERLDPKTLATASCVCKSWSLSMASDHLWEPIFTANFPS 60
           MAQ L     SPPPWEV+LLVAERLDP+TLATASCVCKSWS+SMAS+HLWEPIF+A FPS
Sbjct: 1   MAQSLQP-PPSPPPWEVLLLVAERLDPRTLATASCVCKSWSISMASNHLWEPIFSAKFPS 60

Query: 61  LFNLATAPAT-SPHVSFRRLFGLGYIAAARRRPAQSKPSLSLSDLVFVISISFTNKDRCS 120
           L NLA + AT SP +SF RLFGLG+ AAARRRPA SKP LSLSDL+FVIS+S T+K    
Sbjct: 61  LTNLAASSATASPPLSFCRLFGLGHTAAARRRPAPSKPILSLSDLIFVISVSGTDKADGC 120

Query: 121 IACDGVNKEEKAPRPQSSLTIVKHGEELPVDRNGLFKFDINLNENGDGAPVVIGAREEVE 180
           I   G  KEEK   P+   TIVKHG EL VDRNGLFKFDIN+++ GD A VVIGA  EVE
Sbjct: 121 IVGGGATKEEK-DVPELMFTIVKHGGELVVDRNGLFKFDINVSK-GDEA-VVIGAGGEVE 180

Query: 181 VVWNIVLEGWRGVFTMIECGGRVGMAPRADGWFSEELPAAGCCPNVVSGGIVGDLRLGLC 240
           VVWN+VLEGWR VFTMIECGGRVGMAPRADGWFSEELPAAGCCP  VSGGIV DL+LG+C
Sbjct: 181 VVWNVVLEGWRAVFTMIECGGRVGMAPRADGWFSEELPAAGCCPKEVSGGIVADLKLGVC 240

Query: 241 --GNGGGSNKVRIESVSVGMMSVVSWRYVSLDDGLMYLQHFLFN 282
             G GG +++VRIESVSVGMMSV+SWRYVSLDDGLMYLQHFLFN
Sbjct: 241 KNGEGGKNDEVRIESVSVGMMSVLSWRYVSLDDGLMYLQHFLFN 280

BLAST of CSPI03G24370 vs. NCBI nr
Match: XP_031740421.1 (probable F-box protein At5g04010 [Cucumis sativus] >KAE8649452.1 hypothetical protein Csa_018886 [Cucumis sativus])

HSP 1 Score: 580.1 bits (1494), Expect = 1.0e-161
Identity = 284/284 (100.00%), Postives = 284/284 (100.00%), Query Frame = 0

Query: 1   MAQPLPSLSSSPPPWEVILLVAERLDPKTLATASCVCKSWSLSMASDHLWEPIFTANFPS 60
           MAQPLPSLSSSPPPWEVILLVAERLDPKTLATASCVCKSWSLSMASDHLWEPIFTANFPS
Sbjct: 1   MAQPLPSLSSSPPPWEVILLVAERLDPKTLATASCVCKSWSLSMASDHLWEPIFTANFPS 60

Query: 61  LFNLATAPATSPHVSFRRLFGLGYIAAARRRPAQSKPSLSLSDLVFVISISFTNKDRCSI 120
           LFNLATAPATSPHVSFRRLFGLGYIAAARRRPAQSKPSLSLSDLVFVISISFTNKDRCSI
Sbjct: 61  LFNLATAPATSPHVSFRRLFGLGYIAAARRRPAQSKPSLSLSDLVFVISISFTNKDRCSI 120

Query: 121 ACDGVNKEEKAPRPQSSLTIVKHGEELPVDRNGLFKFDINLNENGDGAPVVIGAREEVEV 180
           ACDGVNKEEKAPRPQSSLTIVKHGEELPVDRNGLFKFDINLNENGDGAPVVIGAREEVEV
Sbjct: 121 ACDGVNKEEKAPRPQSSLTIVKHGEELPVDRNGLFKFDINLNENGDGAPVVIGAREEVEV 180

Query: 181 VWNIVLEGWRGVFTMIECGGRVGMAPRADGWFSEELPAAGCCPNVVSGGIVGDLRLGLCG 240
           VWNIVLEGWRGVFTMIECGGRVGMAPRADGWFSEELPAAGCCPNVVSGGIVGDLRLGLCG
Sbjct: 181 VWNIVLEGWRGVFTMIECGGRVGMAPRADGWFSEELPAAGCCPNVVSGGIVGDLRLGLCG 240

Query: 241 NGGGSNKVRIESVSVGMMSVVSWRYVSLDDGLMYLQHFLFNANV 285
           NGGGSNKVRIESVSVGMMSVVSWRYVSLDDGLMYLQHFLFNANV
Sbjct: 241 NGGGSNKVRIESVSVGMMSVVSWRYVSLDDGLMYLQHFLFNANV 284

BLAST of CSPI03G24370 vs. NCBI nr
Match: XP_008449214.1 (PREDICTED: probable F-box protein At5g04010 [Cucumis melo] >KAA0047228.1 putative F-box protein [Cucumis melo var. makuwa] >TYK21759.1 putative F-box protein [Cucumis melo var. makuwa])

HSP 1 Score: 545.8 bits (1405), Expect = 2.2e-151
Identity = 269/284 (94.72%), Postives = 276/284 (97.18%), Query Frame = 0

Query: 1   MAQPLPSLSSSPPPWEVILLVAERLDPKTLATASCVCKSWSLSMASDHLWEPIFTANFPS 60
           MAQPLPSLSSSPPPWEVILLVAERLDPKTLATASCVCKSWSLSMASDHLWEPIFTANFPS
Sbjct: 1   MAQPLPSLSSSPPPWEVILLVAERLDPKTLATASCVCKSWSLSMASDHLWEPIFTANFPS 60

Query: 61  LFNLATAPATSPHVSFRRLFGLGYIAAARRRPAQSKPSLSLSDLVFVISISFTNKDRCSI 120
           LFNLA+AP +SP VSFRRLFGLGYIAAARRRPAQ KP+LSLSDLVFVISISFT+KD+  I
Sbjct: 61  LFNLASAPTSSPPVSFRRLFGLGYIAAARRRPAQLKPTLSLSDLVFVISISFTDKDQSII 120

Query: 121 ACDGVNKEEKAPRPQSSLTIVKHGEELPVDRNGLFKFDINLNENGDGAPVVIGAREEVEV 180
           ACDGVNKEEKAPRPQSSLT+VKHGEELPV+RNGLFKFDINLNENG  APVVIGAREEVEV
Sbjct: 121 ACDGVNKEEKAPRPQSSLTVVKHGEELPVERNGLFKFDINLNENG-AAPVVIGAREEVEV 180

Query: 181 VWNIVLEGWRGVFTMIECGGRVGMAPRADGWFSEELPAAGCCPNVVSGGIVGDLRLGLCG 240
           VWNIVLEGWRGVFTMIECGGRVGMAPRADGWFSEELPAAGCCPN VSGGIVGDLRLGLCG
Sbjct: 181 VWNIVLEGWRGVFTMIECGGRVGMAPRADGWFSEELPAAGCCPNAVSGGIVGDLRLGLCG 240

Query: 241 NGGGSNKVRIESVSVGMMSVVSWRYVSLDDGLMYLQHFLFNANV 285
           NGGGSNKVRIESVSVGMMSVVSWRYVSLDDGLMYLQHFLFNANV
Sbjct: 241 NGGGSNKVRIESVSVGMMSVVSWRYVSLDDGLMYLQHFLFNANV 283

BLAST of CSPI03G24370 vs. NCBI nr
Match: XP_038883618.1 (probable F-box protein At5g04010 [Benincasa hispida])

HSP 1 Score: 454.5 bits (1168), Expect = 6.6e-124
Identity = 236/288 (81.94%), Postives = 256/288 (88.89%), Query Frame = 0

Query: 1   MAQPLPSLSSSPPPWEVILLVAERLDPKTLATASCVCKSWSLSMASDHLWEPIFTANFPS 60
           MA PLPSL SSPPPWEV+LLVAERLDPKTLATASCVCK WS+SMASDHLWEPIFTANFPS
Sbjct: 1   MAPPLPSL-SSPPPWEVLLLVAERLDPKTLATASCVCKFWSISMASDHLWEPIFTANFPS 60

Query: 61  LFNLA--TAPATSPHVSFRRLFGLGYIAAARRRPAQSKPSLSLSDLVFVISISFTNKDRC 120
           L NLA  ++  TSP VSFRRLFGLG+ AAARRRPA SKP+LSLSDL+FVISIS T+KDR 
Sbjct: 61  LSNLAISSSAPTSPPVSFRRLFGLGHTAAARRRPAPSKPTLSLSDLIFVISISSTDKDRY 120

Query: 121 SIACDGVNKEEKAPRPQSSLTIVKHGEELPVDRNGLFKFDINLN-ENGD-GAPVVIGARE 180
            +  + V K +KAP PQS LT+VKHG EL VDRNGLFKFDINL+ +NG+ G PVVIG RE
Sbjct: 121 IVGHELVEK-DKAP-PQSILTVVKHGGELAVDRNGLFKFDINLDGDNGNKGGPVVIGGRE 180

Query: 181 EVEVVWNIVLEGWRGVFTMIECGGRVGMAPRADGWFSEELPAAGCCPNVVSGGIVGDLRL 240
           EVEVVWN+VLEGWRGVFTMIECGGRVGMAPRADGWFSEELPAAGCC NVVSGGIV DL+L
Sbjct: 181 EVEVVWNLVLEGWRGVFTMIECGGRVGMAPRADGWFSEELPAAGCCSNVVSGGIVADLKL 240

Query: 241 GLCGNGGGSNKVRIESVSVGMMSVVSWRYVSLDDGLMYLQHFLFNANV 285
           GL GN GG +KVRIESVSVGMMS+VSWRYVSLDDGLMYLQHFLFNA+V
Sbjct: 241 GLRGNNGGGSKVRIESVSVGMMSIVSWRYVSLDDGLMYLQHFLFNASV 285

BLAST of CSPI03G24370 vs. NCBI nr
Match: XP_022925168.1 (probable F-box protein At5g04010 [Cucurbita moschata])

HSP 1 Score: 410.2 bits (1053), Expect = 1.4e-110
Identity = 217/284 (76.41%), Postives = 237/284 (83.45%), Query Frame = 0

Query: 1   MAQPLPSLSSSPPPWEVILLVAERLDPKTLATASCVCKSWSLSMASDHLWEPIFTANFPS 60
           MAQ L     SPPPWEV+LLVAERLDP+TLATASCVCKSWS+SMAS++LWEPIF+A FPS
Sbjct: 1   MAQSLQP-PPSPPPWEVLLLVAERLDPRTLATASCVCKSWSISMASNNLWEPIFSAKFPS 60

Query: 61  LFNLATAPA-TSPHVSFRRLFGLGYIAAARRRPAQSKPSLSLSDLVFVISISFTNKDRCS 120
           L NLA + A TSP VSFRRLFGLG+ AAARRRPA SKP LSLSDLVFVIS+S T+K    
Sbjct: 61  LTNLAASSATTSPPVSFRRLFGLGHTAAARRRPAPSKPILSLSDLVFVISVSGTDKANGC 120

Query: 121 IACDGVNKEEKAPRPQSSLTIVKHGEELPVDRNGLFKFDINLNENGDGAPVVIGAREEVE 180
           I   G  KEEK   P+   T+VKHG EL VDRN LFKFDIN++ NGD   VVIGA EEVE
Sbjct: 121 IVSGGTKKEEK-DVPEPMFTVVKHGGELAVDRNRLFKFDINVS-NGDEV-VVIGAGEEVE 180

Query: 181 VVWNIVLEGWRGVFTMIECGGRVGMAPRADGWFSEELPAAGCCPNVVSGGIVGDLRLGLC 240
           VVWN+VLEGWR VFTMIECGGRVGMAPRADGWFSEELPAAGCCP  VSGGIV DL+LG+C
Sbjct: 181 VVWNVVLEGWRAVFTMIECGGRVGMAPRADGWFSEELPAAGCCPKEVSGGIVADLKLGVC 240

Query: 241 --GNGGGSNKVRIESVSVGMMSVVSWRYVSLDDGLMYLQHFLFN 282
             G GG ++KVRIESVSVGMMSV+SWRYVSLDDGLMYLQHFLFN
Sbjct: 241 KNGEGGKNDKVRIESVSVGMMSVLSWRYVSLDDGLMYLQHFLFN 280

BLAST of CSPI03G24370 vs. NCBI nr
Match: XP_023518459.1 (probable F-box protein At5g04010 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 407.5 bits (1046), Expect = 9.3e-110
Identity = 215/285 (75.44%), Postives = 238/285 (83.51%), Query Frame = 0

Query: 1   MAQPLPSLSSSPPPWEVILLVAERLDPKTLATASCVCKSWSLSMASDHLWEPIFTANFPS 60
           MAQ L     SPPPWEV+LLVAERLDP+TLATASCVCKSWS+SMAS++LWEPIF+A FPS
Sbjct: 1   MAQSLQP-PPSPPPWEVLLLVAERLDPRTLATASCVCKSWSISMASNNLWEPIFSAKFPS 60

Query: 61  LFNLATAPA-TSPHVSFRRLFGLGYIAAARRRPAQSKPSLSLSDLVFVISISFTNK-DRC 120
           L NLA + A TSP VSFRRLFGLG+ AAARRRPA SKP L LSDLVFVIS+S T+K DRC
Sbjct: 61  LTNLAASSATTSPPVSFRRLFGLGHTAAARRRPAPSKPILCLSDLVFVISVSSTDKADRC 120

Query: 121 SIACDGVNKEEKAPRPQSSLTIVKHGEELPVDRNGLFKFDINLNENGDGAPVVIGAREEV 180
            +   G  KEEK   P+   T+VKHG EL VDRNGLFKFDIN++ + +   VVIGA EEV
Sbjct: 121 IVGA-GAKKEEK-DVPEPMFTVVKHGGELAVDRNGLFKFDINVSNSDE--VVVIGAGEEV 180

Query: 181 EVVWNIVLEGWRGVFTMIECGGRVGMAPRADGWFSEELPAAGCCPNVVSGGIVGDLRLGL 240
            VVWN+VLEGWR VFTMIECGGRVGMAPRADGWFSEELPAAGCCP  VSGGIV DL+LG+
Sbjct: 181 GVVWNVVLEGWRAVFTMIECGGRVGMAPRADGWFSEELPAAGCCPKEVSGGIVADLKLGV 240

Query: 241 C--GNGGGSNKVRIESVSVGMMSVVSWRYVSLDDGLMYLQHFLFN 282
           C  G GG ++KVRIESVSVGMMSV+SWRYVSLDDGLMYLQHFLFN
Sbjct: 241 CKNGEGGKNDKVRIESVSVGMMSVLSWRYVSLDDGLMYLQHFLFN 280

BLAST of CSPI03G24370 vs. TAIR 10
Match: AT5G04010.1 (F-box family protein )

HSP 1 Score: 109.8 bits (273), Expect = 3.7e-24
Identity = 78/272 (28.68%), Postives = 130/272 (47.79%), Query Frame = 0

Query: 11  SPPPWEVILLVAERLDPKTLATASCVCKSWSLSMASDHLWEPIFTANFPSLFNLATAPAT 70
           SPP WE++ LV   +DP++LA ASCV  +WS   +S+ LW+ +            T    
Sbjct: 51  SPPSWEILCLVGPYMDPESLAVASCVSTTWSKCFSSEDLWKSLPATRHSIFAKAITKEGA 110

Query: 71  SP-HVSFRRLFGLGYIAAARRRPAQ-SKPSLSLSDLVFVISISFTNKDRCSIACDGVNKE 130
            P  +S++RL      AA RRR  Q ++P +SLSDLVF++ +S  +K+            
Sbjct: 111 KPAWLSYKRLISEAESAAKRRRNNQPAEPKISLSDLVFIVHVSAGSKE------------ 170

Query: 131 EKAPRPQSSLTIVKHGEELPVDRNGLFKFDINLNENGDGAPVVIGAREEVEVVWNIVLEG 190
                      +VK G++L    N  F+ + ++ ++G  A +     ++V + WN+VL  
Sbjct: 171 ---------AVVVKQGKDLVFGSNERFQIEADVRDSGFTADM-----KDVSMSWNVVLRN 230

Query: 191 WRGVFTMIECGGRVGMAPRADGWFSEELPAAGCCPNVVSG-GIVGDLRLGLCGNGGGSNK 250
           +  +F M E    +       GWF++ELP  G       G  +VG+++          N+
Sbjct: 231 YERMFLMSET--VIKSLDSMIGWFTDELP--GTKNRYCDGSNLVGEVKPSF-------NE 285

Query: 251 VRIESVSVGMMSVVSWRYVSLDDGLMYLQHFL 280
             ++ V   +    +W+ + +DD L YLQ FL
Sbjct: 291 DVLDKVVFAIADSRNWKSLFVDDVLRYLQCFL 285

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q5EAF65.3e-2328.68Probable F-box protein At5g04010 OS=Arabidopsis thaliana OX=3702 GN=NSFBx PE=2 S... [more]
Match NameE-valueIdentityDescription
A0A0A0LDE55.1e-162100.00F-box domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G497390 PE=4 ... [more]
A0A5D3DEJ61.1e-15194.72Putative F-box protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1... [more]
A0A1S3BLJ11.1e-15194.72probable F-box protein At5g04010 OS=Cucumis melo OX=3656 GN=LOC103491161 PE=4 SV... [more]
A0A6J1EEG36.9e-11176.41probable F-box protein At5g04010 OS=Cucurbita moschata OX=3662 GN=LOC111432489 P... [more]
A0A6J1HMX92.9e-10975.70probable F-box protein At5g04010 OS=Cucurbita maxima OX=3661 GN=LOC111466077 PE=... [more]
Match NameE-valueIdentityDescription
XP_031740421.11.0e-161100.00probable F-box protein At5g04010 [Cucumis sativus] >KAE8649452.1 hypothetical pr... [more]
XP_008449214.12.2e-15194.72PREDICTED: probable F-box protein At5g04010 [Cucumis melo] >KAA0047228.1 putativ... [more]
XP_038883618.16.6e-12481.94probable F-box protein At5g04010 [Benincasa hispida][more]
XP_022925168.11.4e-11076.41probable F-box protein At5g04010 [Cucurbita moschata][more]
XP_023518459.19.3e-11075.44probable F-box protein At5g04010 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT5G04010.13.7e-2428.68F-box family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D1.20.1280.50coord: 8..95
e-value: 9.9E-8
score: 33.8
NoneNo IPR availablePANTHERPTHR12874:SF16F-BOX PROTEINcoord: 9..268
IPR001810F-box domainPFAMPF00646F-boxcoord: 14..52
e-value: 5.9E-5
score: 22.8
IPR045118F-box only protein FBXO9/FBXO48PANTHERPTHR12874F-BOX ONLY PROTEIN 48-RELATEDcoord: 9..268
IPR036047F-box-like domain superfamilySUPERFAMILY81383F-box domaincoord: 14..66

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI03G24370.1CSPI03G24370.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0031146 SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
cellular_component GO:0019005 SCF ubiquitin ligase complex
molecular_function GO:0005515 protein binding