CSPI03G23650 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI03G23650
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Descriptionindole-3-acetic acid-amido synthetase GH3.6
LocationChr3: 20611942 .. 20615600 (-)
RNA-Seq ExpressionCSPI03G23650
SyntenyCSPI03G23650
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CAACCATCGTTTCTAAACACACATACTCACACACACCCTTCTCTTAATCACTCCTCTTCTCTTCTTTTCTACAACATCATGCCTGAAGAAACTCCTCAAAACTCCTCATTTCTCTCCACTACTGAATACTACAAACTGTTGGAGAAAAACAAGAAGACTCTTCAATTCATTGAAGATGTCACTGCCAACGCCGATCAAGTCCAGCGTCGGGTCCTCACCGAGATCCTTTCCCGCAATGCCGGCGTTGAGTACCTCCGTCGACACGGGCTCGGTGGCGCCACCGACTGTAAGACCTTCAAAAATACCCTTCCTCTTATCTCCTACGAGGATATTCAACCTGATATCAACCGTATTGCCAATGGGGACTCCTCTTCCATTCTCTGTTCCTCTCCCATTTCTGAGTTTCTCACAAGGTGGGTTTCTCTAATTTTTTTTTTTTTTTTATATATTCTCCCCCTCTTTTCATTTCACCCTTTCCTCAAATGGGTCTCTGTTTTTCTTGTTTCTTTTTCCTCGTATAGGATGTAAAAACGCTAAATTTACCGTAACTCATCCACTTGAACGCTTTTATTTTCAATTTTAAGTAAACGTCATCATGTTTATCGTGATGTCCACTATCCTCCTCAACTTGCTTTGAGTTTCCTTATGCTCTAACTCGTAACTTTAGAGGTCCTATTTTTGAATTGCGGTAAACATCATCTGGTTAGCCGTAATCCATCAACTCATTTATTTATCTCTTTATTTTTGGAACTCTTTTCAACTTAATTTTTGAAATCGACAGTAATTTCAACACAAACCCATAAGTGATGTCATGTTGAAATCATCAATAATATCATTAGTTTGCCGAAATATCCATGCGCTCAAACTTTTTCTTATTTCCGTATGCTTTAAATTCATCAATATAATTGGGTAAATGTCACATGACTACTAAAACCCATCGGCTTCAAGGACAATTATATTTAACATGGTAACATGTATGATTTTTTGTTTCTAAAACCCATCAACCAAACAATACTTACAATACAAATCCAGAAGAAGAGGGGCGTCAGTTATAGCGAAACTGCTAAAAGAGATTATGTATTCAAAGAAAATAGTTTTAAATGAAAGGTTTTGAGTTTATGGATGATACTAAATTTATGGAATGGTTCATGTCCAGCTCGGGGACATCAGGAGGAGAGAGAAAGCTAATGCCAACAATAGAAGAAGAATTAGAAAGAAGATCATCGCTCTACAGTTTACTAATGCCAGTAATGAGCCAATTCATAGAAGGCTTAGAAAAAGGGAAAGGAATGTACTTTCTGTTCATAAAATCAGAATCCAAAACCCCAAGTGGACTACTCGCTCGACCAGTACTAACAAGCTATTACAAAAGCCCCCACTTCAAGAACCGCCCATTCGACCCATACACCAATTACACAAGCCCAAACGAAGCCATCCTCTGTTCCGACTCATACCAAAGCATGTATGCCCAGCTCCTCTGCGGCCTCCTCCACCGCACTGACGTCCTCCGTGTTGGAGCTGTCTTCGCCTCCGGCTTCATCCGAGCCATCCGGTTCCTGGAAAAGCACTACACATTGCTCTGCCACGACATTCGAACTGGGACTCTAAACTCGGAGATCACGGATCAGGGGATTCGGGAATCGGTGAGGAAGATTCTGAAGGCAGATGGGAAATTGGCGGATTTTGTGGAAGGGGAATGTGGGAAGAAATGTTGGGGAGGGATTATAAGTAGGATATGGCCGAATACGAAGTACGTGGATGTGATTGTAACGGGGACAATGTCGCAGTATATCGGGACATTGGATTATTATAGTAATGGTTTGCCAATTGTTTGCACAATGTATGCTTCATCGGAGTGTTATTTTGGAGTGAATTTGAAGCCCATGTGTAAACCAGGGGAAGTGGCATATACGCTTATACCAACGATGGCGTATTTTGAGTTTCTTCCTGTGAATAGAAGTAAAGAGAAGGAGTTGGAGAAGGAGCTTGTGGATCTTGTTGATGTCAAGCTTGGCCATGAGTATGAGCTTGTTGTTACCACATATTCAGGTTAGTATTTTTTTTTTCTTTTTTTTTTGAAAAAAACTTTAATTTTAAGATTTGTCTGTCCAAATCCGGGAGGAAATGGAGAAAGAAAACGGGTGTTCCACAAAACTAACAAGTAGATGAAGTTTAATAATACACAATTTTATTAAACATAACAAAGTTTAGTCTAAAGTTTGTATCATTTCTTTTCTTTTCTTTTTTTCTTTTTAGTAAGGGAACATTTTTCTTTCCTCAATTCCATATTTGGAAATATATATTTTTTTAGAAGCAACATTTTAGGATTTTGAAACATGTATGAGAAGTGGGTAAATTTATTTATTTAAACCACATAAATCCAAAACCATTAGGTAATTAAAGTCTAATTTACTAAGTATTTTGTTTTTTCTTTACAGTTTTTTTTTTTTAAAAAAAACAATTAAACTTATTTACTTTCCACTACTAATAAGGATTTGCATTTTTCTAAATACAAAAATGGTAGTCTTCCTCAAACTTAAAAAAACAAAAACAAAAACAAATTAAAAAATATATATTTTACTTTCTTTTTCTTTCAAACTTTGAATTAGTTTACCGATAAAAAGTAGATAAAAAAGGAACCAATTAGAGGTAGAACGAGTGATTACTAAATGAATTTTCAAAAAGAGTAAAAATCAAGTTGTTATCAAACATTTCTCTAAATAATATAACAAGAACCATGTTTTTAGTTTTTGAAAATTTAGCTTATAAATATCACTACTTATTCTTATTGATTTATTTCTTAATTTATGATTTATCTCACTGATTCAAAAGCAGACATTAAATAGCACATGATATGGTTTGTATGATTAATGTTTTTTCTGTTATTTACCTTCAGGATTGTATAGGTATAGAGTGGGAGATATACTAAGAGTGGCAGGGTTCAAGAACAATGCACCACAATTCAATTTCATATGCAGAAAAAATGTTGCTTTAAGCATAGATTCAGACAAAACAGATGAGGTTGAATTACAAAATGCTGTAAAAAACGCCTTAACCCATTTGATACCATTTGATGTCACTTTGAGTGAGTACACAAGCTGTGTTGACACTTCAAACATCCCTGGCCATTATGTTCTCTATTGGGAACTAACTCTAAACAATGAAATCAAATCAACCCCAATTCCACCCTCTGTCTTTGAGGATTGTTGCTTTGCTATCGAAGAGTCTCTTAATGCCGTGTACCGTCAAGGTCGGGTCTCCGATAAGTCCATTGGTCCTCTCGAGATCAAGATCGTCGAAACTGGAACATTCGACAAGCTCATGGACTTTGCAATTAGCTTAGGAGCATCAATAAATCAGTATAAGACACCAAGATGTGTCAAGTATGAACCCATAATTCAACTATTGAGTTCTAGGGTTGTGTCAAATTATTTCAGCCCCAAATGCCCTAAATGGGTTCCAAAGCAATGGATCTGAAAGTGGAAATTTTAATTATTGGATTGTTGTATCAATAGGGAATATTTAATCACCCTAGAGATTTTAATGGTTTTCTTTTTCTTGCATCCTTTCTGAAATTTTAGGCTAATTTGAAGGTTAATAGACAATGTACAAAAGTAGTTCTTCTGTTGTTCAACAAATTAAAGTAATCTTTTTTCTTT

mRNA sequence

CAACCATCGTTTCTAAACACACATACTCACACACACCCTTCTCTTAATCACTCCTCTTCTCTTCTTTTCTACAACATCATGCCTGAAGAAACTCCTCAAAACTCCTCATTTCTCTCCACTACTGAATACTACAAACTGTTGGAGAAAAACAAGAAGACTCTTCAATTCATTGAAGATGTCACTGCCAACGCCGATCAAGTCCAGCGTCGGGTCCTCACCGAGATCCTTTCCCGCAATGCCGGCGTTGAGTACCTCCGTCGACACGGGCTCGGTGGCGCCACCGACTGTAAGACCTTCAAAAATACCCTTCCTCTTATCTCCTACGAGGATATTCAACCTGATATCAACCGTATTGCCAATGGGGACTCCTCTTCCATTCTCTGTTCCTCTCCCATTTCTGAGTTTCTCACAAGCTCGGGGACATCAGGAGGAGAGAGAAAGCTAATGCCAACAATAGAAGAAGAATTAGAAAGAAGATCATCGCTCTACAGTTTACTAATGCCAGTAATGAGCCAATTCATAGAAGGCTTAGAAAAAGGGAAAGGAATGTACTTTCTGTTCATAAAATCAGAATCCAAAACCCCAAGTGGACTACTCGCTCGACCAGTACTAACAAGCTATTACAAAAGCCCCCACTTCAAGAACCGCCCATTCGACCCATACACCAATTACACAAGCCCAAACGAAGCCATCCTCTGTTCCGACTCATACCAAAGCATGTATGCCCAGCTCCTCTGCGGCCTCCTCCACCGCACTGACGTCCTCCGTGTTGGAGCTGTCTTCGCCTCCGGCTTCATCCGAGCCATCCGGTTCCTGGAAAAGCACTACACATTGCTCTGCCACGACATTCGAACTGGGACTCTAAACTCGGAGATCACGGATCAGGGGATTCGGGAATCGGTGAGGAAGATTCTGAAGGCAGATGGGAAATTGGCGGATTTTGTGGAAGGGGAATGTGGGAAGAAATGTTGGGGAGGGATTATAAGTAGGATATGGCCGAATACGAAGTACGTGGATGTGATTGTAACGGGGACAATGTCGCAGTATATCGGGACATTGGATTATTATAGTAATGGTTTGCCAATTGTTTGCACAATGTATGCTTCATCGGAGTGTTATTTTGGAGTGAATTTGAAGCCCATGTGTAAACCAGGGGAAGTGGCATATACGCTTATACCAACGATGGCGTATTTTGAGTTTCTTCCTGTGAATAGAAGTAAAGAGAAGGAGTTGGAGAAGGAGCTTGTGGATCTTGTTGATGTCAAGCTTGGCCATGAGTATGAGCTTGTTGTTACCACATATTCAGGATTGTATAGGTATAGAGTGGGAGATATACTAAGAGTGGCAGGGTTCAAGAACAATGCACCACAATTCAATTTCATATGCAGAAAAAATGTTGCTTTAAGCATAGATTCAGACAAAACAGATGAGGTTGAATTACAAAATGCTGTAAAAAACGCCTTAACCCATTTGATACCATTTGATGTCACTTTGAGTGAGTACACAAGCTGTGTTGACACTTCAAACATCCCTGGCCATTATGTTCTCTATTGGGAACTAACTCTAAACAATGAAATCAAATCAACCCCAATTCCACCCTCTGTCTTTGAGGATTGTTGCTTTGCTATCGAAGAGTCTCTTAATGCCGTGTACCGTCAAGGTCGGGTCTCCGATAAGTCCATTGGTCCTCTCGAGATCAAGATCGTCGAAACTGGAACATTCGACAAGCTCATGGACTTTGCAATTAGCTTAGGAGCATCAATAAATCAGTATAAGACACCAAGATGTGTCAAGTATGAACCCATAATTCAACTATTGAGTTCTAGGGTTGTGTCAAATTATTTCAGCCCCAAATGCCCTAAATGGGTTCCAAAGCAATGGATCTGAAAGTGGAAATTTTAATTATTGGATTGTTGTATCAATAGGGAATATTTAATCACCCTAGAGATTTTAATGGTTTTCTTTTTCTTGCATCCTTTCTGAAATTTTAGGCTAATTTGAAGGTTAATAGACAATGTACAAAAGTAGTTCTTCTGTTGTTCAACAAATTAAAGTAATCTTTTTTCTTT

Coding sequence (CDS)

ATGCCTGAAGAAACTCCTCAAAACTCCTCATTTCTCTCCACTACTGAATACTACAAACTGTTGGAGAAAAACAAGAAGACTCTTCAATTCATTGAAGATGTCACTGCCAACGCCGATCAAGTCCAGCGTCGGGTCCTCACCGAGATCCTTTCCCGCAATGCCGGCGTTGAGTACCTCCGTCGACACGGGCTCGGTGGCGCCACCGACTGTAAGACCTTCAAAAATACCCTTCCTCTTATCTCCTACGAGGATATTCAACCTGATATCAACCGTATTGCCAATGGGGACTCCTCTTCCATTCTCTGTTCCTCTCCCATTTCTGAGTTTCTCACAAGCTCGGGGACATCAGGAGGAGAGAGAAAGCTAATGCCAACAATAGAAGAAGAATTAGAAAGAAGATCATCGCTCTACAGTTTACTAATGCCAGTAATGAGCCAATTCATAGAAGGCTTAGAAAAAGGGAAAGGAATGTACTTTCTGTTCATAAAATCAGAATCCAAAACCCCAAGTGGACTACTCGCTCGACCAGTACTAACAAGCTATTACAAAAGCCCCCACTTCAAGAACCGCCCATTCGACCCATACACCAATTACACAAGCCCAAACGAAGCCATCCTCTGTTCCGACTCATACCAAAGCATGTATGCCCAGCTCCTCTGCGGCCTCCTCCACCGCACTGACGTCCTCCGTGTTGGAGCTGTCTTCGCCTCCGGCTTCATCCGAGCCATCCGGTTCCTGGAAAAGCACTACACATTGCTCTGCCACGACATTCGAACTGGGACTCTAAACTCGGAGATCACGGATCAGGGGATTCGGGAATCGGTGAGGAAGATTCTGAAGGCAGATGGGAAATTGGCGGATTTTGTGGAAGGGGAATGTGGGAAGAAATGTTGGGGAGGGATTATAAGTAGGATATGGCCGAATACGAAGTACGTGGATGTGATTGTAACGGGGACAATGTCGCAGTATATCGGGACATTGGATTATTATAGTAATGGTTTGCCAATTGTTTGCACAATGTATGCTTCATCGGAGTGTTATTTTGGAGTGAATTTGAAGCCCATGTGTAAACCAGGGGAAGTGGCATATACGCTTATACCAACGATGGCGTATTTTGAGTTTCTTCCTGTGAATAGAAGTAAAGAGAAGGAGTTGGAGAAGGAGCTTGTGGATCTTGTTGATGTCAAGCTTGGCCATGAGTATGAGCTTGTTGTTACCACATATTCAGGATTGTATAGGTATAGAGTGGGAGATATACTAAGAGTGGCAGGGTTCAAGAACAATGCACCACAATTCAATTTCATATGCAGAAAAAATGTTGCTTTAAGCATAGATTCAGACAAAACAGATGAGGTTGAATTACAAAATGCTGTAAAAAACGCCTTAACCCATTTGATACCATTTGATGTCACTTTGAGTGAGTACACAAGCTGTGTTGACACTTCAAACATCCCTGGCCATTATGTTCTCTATTGGGAACTAACTCTAAACAATGAAATCAAATCAACCCCAATTCCACCCTCTGTCTTTGAGGATTGTTGCTTTGCTATCGAAGAGTCTCTTAATGCCGTGTACCGTCAAGGTCGGGTCTCCGATAAGTCCATTGGTCCTCTCGAGATCAAGATCGTCGAAACTGGAACATTCGACAAGCTCATGGACTTTGCAATTAGCTTAGGAGCATCAATAAATCAGTATAAGACACCAAGATGTGTCAAGTATGAACCCATAATTCAACTATTGAGTTCTAGGGTTGTGTCAAATTATTTCAGCCCCAAATGCCCTAAATGGGTTCCAAAGCAATGGATCTGA

Protein sequence

MPEETPQNSSFLSTTEYYKLLEKNKKTLQFIEDVTANADQVQRRVLTEILSRNAGVEYLRRHGLGGATDCKTFKNTLPLISYEDIQPDINRIANGDSSSILCSSPISEFLTSSGTSGGERKLMPTIEEELERRSSLYSLLMPVMSQFIEGLEKGKGMYFLFIKSESKTPSGLLARPVLTSYYKSPHFKNRPFDPYTNYTSPNEAILCSDSYQSMYAQLLCGLLHRTDVLRVGAVFASGFIRAIRFLEKHYTLLCHDIRTGTLNSEITDQGIRESVRKILKADGKLADFVEGECGKKCWGGIISRIWPNTKYVDVIVTGTMSQYIGTLDYYSNGLPIVCTMYASSECYFGVNLKPMCKPGEVAYTLIPTMAYFEFLPVNRSKEKELEKELVDLVDVKLGHEYELVVTTYSGLYRYRVGDILRVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNALTHLIPFDVTLSEYTSCVDTSNIPGHYVLYWELTLNNEIKSTPIPPSVFEDCCFAIEESLNAVYRQGRVSDKSIGPLEIKIVETGTFDKLMDFAISLGASINQYKTPRCVKYEPIIQLLSSRVVSNYFSPKCPKWVPKQWI*
Homology
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BLAST of CSPI03G23650 vs. ExPASy Swiss-Prot
Match: Q9LSQ4 (Indole-3-acetic acid-amido synthetase GH3.6 OS=Arabidopsis thaliana OX=3702 GN=GH3.6 PE=1 SV=1)

HSP 1 Score: 963.0 bits (2488), Expect = 1.6e-279
Identity = 472/597 (79.06%), Postives = 523/597 (87.60%), Query Frame = 0 

Query: 20  LLEKNKKTLQFIEDVTANADQVQRRVLTEILSRNAGVEYLRRHGLGGATDCKTFKNTLPL 79
           L EKNK  LQFIEDVT NAD VQRRVL EILSRNA VEYL+RHGL G TD +TFK+ +P+
Sbjct: 17  LAEKNKNKLQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHGLEGRTDRETFKHIMPV 76

Query: 80  ISYEDIQPDINRIANGDSSSILCSSPISEFLTSSGTSGGERKLMPTIEEELERRSSLYSL 139
           ++YEDIQP+INRIANGD S +LCS+PISEFLTSSGTSGGERKLMPTIEEEL+RRS LYSL
Sbjct: 77  VTYEDIQPEINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSL 136

Query: 140 LMPVMSQFIEGLEKGKGMYFLFIKSESKTPSGLLARPVLTSYYKSPHFKNRPFDPYTNYT 199
           LMPVM QF+ GL+KGKGMYFLFIKSESKTP GL ARPVLTSYYKS HFKNRP+DPYTNYT
Sbjct: 137 LMPVMDQFVPGLDKGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKNRPYDPYTNYT 196

Query: 200 SPNEAILCSDSYQSMYAQLLCGLLHRTDVLRVGAVFASGFIRAIRFLEKHYTLLCHDIRT 259
           SPN+ ILCSDSYQSMY+Q+LCGL    +VLRVGAVFASGFIRAI+FLEKH+  L  DIRT
Sbjct: 197 SPNQTILCSDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAIKFLEKHWPELARDIRT 256

Query: 260 GTLNSEITDQGIRESVRKILKADGKLADFVEGECGKKCWGGIISRIWPNTKYVDVIVTGT 319
           GTL+SEITD  +RE+V +ILK D KLADFVE EC K  W GII+R+WPNTKYVDVIVTGT
Sbjct: 257 GTLSSEITDSSVREAVGEILKPDPKLADFVESECRKTSWQGIITRLWPNTKYVDVIVTGT 316

Query: 320 MSQYIGTLDYYSNGLPIVCTMYASSECYFGVNLKPMCKPGEVAYTLIPTMAYFEFLPVNR 379
           MSQYI TLDYYSNGLP+VCTMYASSECYFGVNL+P+CKP EV+YTLIP MAYFEFLPV+R
Sbjct: 317 MSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPNMAYFEFLPVHR 376

Query: 380 SK-------------EKELEKELVDLVDVKLGHEYELVVTTYSGLYRYRVGDILRVAGFK 439
           +              EKE ++ELVDLVDVKLG EYELVVTTY+GLYRYRVGD+L VAGFK
Sbjct: 377 NSGVTSSISLPKALTEKE-QQELVDLVDVKLGQEYELVVTTYAGLYRYRVGDVLSVAGFK 436

Query: 440 NNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNALTHLIPFDVTLSEYTSCVDTSNIPG 499
           NNAPQF+FICRKNV LSIDSDKTDEVELQNAVKNA+THL+PFD +LSEYTS  DTS+IPG
Sbjct: 437 NNAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIPG 496

Query: 500 HYVLYWELTLNNEIKSTPIPPSVFEDCCFAIEESLNAVYRQGRVSDKSIGPLEIKIVETG 559
           HYVL+WEL LN    +TPIPPSVFEDCC  IEESLN+VYRQGRVSDKSIGPLEIK+VE+G
Sbjct: 497 HYVLFWELCLNG---NTPIPPSVFEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKMVESG 556

Query: 560 TFDKLMDFAISLGASINQYKTPRCVKYEPIIQLLSSRVVSNYFSPKCPKWVP--KQW 602
           TFDKLMD+AISLGASINQYKTPRCVK+ PII+LL+SRVV +YFSPKCPKW P  KQW
Sbjct: 557 TFDKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWSPGHKQW 609

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BLAST of CSPI03G23650 vs. ExPASy Swiss-Prot
Match: O81829 (Indole-3-acetic acid-amido synthetase GH3.5 OS=Arabidopsis thaliana OX=3702 GN=GH3.5 PE=1 SV=1)

HSP 1 Score: 941.0 bits (2431), Expect = 6.5e-273
Identity = 465/595 (78.15%), Postives = 516/595 (86.72%), Query Frame = 0 

Query: 22  EKNKKTLQFIEDVTANADQVQRRVLTEILSRNAGVEYLRRHGLGGATDCKTFKNTLPLIS 81
           +KNK+ LQ IE++T+NADQVQR+VL EIL+RNA VEYLRRH L G TD +TFKN +P+I+
Sbjct: 19  QKNKQKLQLIEELTSNADQVQRQVLEEILTRNADVEYLRRHDLNGRTDRETFKNIMPVIT 78

Query: 82  YEDIQPDINRIANGDSSSILCSSPISEFLTSSGTSGGERKLMPTIEEELERRSSLYSLLM 141
           YEDI+P+INRIANGD S IL S PISEFLTSSGTSGGERKLMPTIEEEL+RRS LYSLLM
Sbjct: 79  YEDIEPEINRIANGDKSPILSSKPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSLLM 138

Query: 142 PVMSQFIEGLEKGKGMYFLFIKSESKTPSGLLARPVLTSYYKSPHFKNRPFDPYTNYTSP 201
           PVMSQF+ GLE GKGMYFLFIKSESKTP GL ARPVLTSYYKS HFK RP+DPYTNYTSP
Sbjct: 139 PVMSQFVPGLENGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKERPYDPYTNYTSP 198

Query: 202 NEAILCSDSYQSMYAQLLCGLLHRTDVLRVGAVFASGFIRAIRFLEKHYTLLCHDIRTGT 261
           NE ILCSDSYQSMY+Q+LCGL    +VLRVGAVFASGFIRAI+FLEKH+  L  DIRTGT
Sbjct: 199 NETILCSDSYQSMYSQMLCGLCQHQEVLRVGAVFASGFIRAIKFLEKHWIELVRDIRTGT 258

Query: 262 LNSEITDQGIRESVRKILKADGKLADFVEGECGKKCWGGIISRIWPNTKYVDVIVTGTMS 321
           L+S ITD  +RE+V KILK   KLADFVE EC K  W GII+R+WPNTKYVDVIVTGTMS
Sbjct: 259 LSSLITDPSVREAVAKILKPSPKLADFVEFECKKSSWQGIITRLWPNTKYVDVIVTGTMS 318

Query: 322 QYIGTLDYYSNGLPIVCTMYASSECYFGVNLKPMCKPGEVAYTLIPTMAYFEFLPVNRSK 381
           QYI TLDYYSNGLP+VCTMYASSECYFGVNL+P+CKP EV+YTLIP+MAYFEFLPV+R+ 
Sbjct: 319 QYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPSMAYFEFLPVHRNN 378

Query: 382 -------------EKELEKELVDLVDVKLGHEYELVVTTYSGLYRYRVGDILRVAGFKNN 441
                        EKE ++ELVDLVDVKLG EYELVVTTY+GL RYRVGD+LRV GFKN 
Sbjct: 379 GVTNSINLPKALTEKE-QQELVDLVDVKLGQEYELVVTTYAGLCRYRVGDLLRVTGFKNK 438

Query: 442 APQFNFICRKNVALSIDSDKTDEVELQNAVKNALTHLIPFDVTLSEYTSCVDTSNIPGHY 501
           APQF+FICRKNV LSIDSDKTDEVELQNAVKNA+THL+PFD +LSEYTS  DTS+IPGHY
Sbjct: 439 APQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIPGHY 498

Query: 502 VLYWELTLNNEIKSTPIPPSVFEDCCFAIEESLNAVYRQGRVSDKSIGPLEIKIVETGTF 561
           VL+WEL L+    +TPIPPSVFEDCC A+EES N VYRQGRVSDKSIGPLEIKIVE GTF
Sbjct: 499 VLFWELCLDG---NTPIPPSVFEDCCLAVEESFNTVYRQGRVSDKSIGPLEIKIVEPGTF 558

Query: 562 DKLMDFAISLGASINQYKTPRCVKYEPIIQLLSSRVVSNYFSPKCPKWVP--KQW 602
           DKLMD+AISLGASINQYKTPRCVK+ PII+LL+SRVV +YFSPKCPKWVP  KQW
Sbjct: 559 DKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWVPGHKQW 609

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BLAST of CSPI03G23650 vs. ExPASy Swiss-Prot
Match: Q8LQM5 (Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.1 PE=2 SV=1)

HSP 1 Score: 825.5 bits (2131), Expect = 4.0e-238
Identity = 398/581 (68.50%), Postives = 471/581 (81.07%), Query Frame = 0 

Query: 26  KTLQFIEDVTANADQVQRRVLTEILSRNAGVEYLRRHGLGGATD-CKTFKNTLPLISYED 85
           + L+FIE VTANA QVQRRVL EIL++NA  EYLRR+G+ G+ D    F+  +PL++YE 
Sbjct: 22  EALEFIEHVTANAGQVQRRVLGEILAQNAPAEYLRRYGIPGSPDVVDAFRRLVPLVTYEG 81

Query: 86  IQPDINRIANGDSSSILCSSPISEFLTSSGTSGGERKLMPTIEEELERRSSLYSLLMPVM 145
           +QPDI RIANGD+S I    PISEFLTSSGTSGGERKLMPTI +E+ RRS LYSLLMPVM
Sbjct: 82  LQPDILRIANGDTSPIFSGKPISEFLTSSGTSGGERKLMPTIADEMNRRSLLYSLLMPVM 141

Query: 146 SQFIEGLEKGKGMYFLFIKSESKTPSGLLARPVLTSYYKSPHFKNRPFDPYTNYTSPNEA 205
           SQ + GL+KGK MY LF+K+ES+TP GL ARPVLTSYY+S  F +RP DPYT+YTSP+EA
Sbjct: 142 SQSVSGLDKGKAMYLLFVKAESRTPGGLAARPVLTSYYRSRQFLDRPRDPYTSYTSPDEA 201

Query: 206 ILCSDSYQSMYAQLLCGLLHRTDVLRVGAVFASGFIRAIRFLEKHYTLLCHDIRTGTLNS 265
           ILC DSYQSMYAQLLCGL+HR DVLRVGAVFASGF+RAI FLEKH+  LCHDIRTG L+ 
Sbjct: 202 ILCVDSYQSMYAQLLCGLVHRADVLRVGAVFASGFLRAIHFLEKHWARLCHDIRTGELDP 261

Query: 266 EITDQGIRESVRKILKADGKLADFVEGECGKKCWGGIISRIWPNTKYVDVIVTGTMSQYI 325
           EITD+ +R++V ++L+AD  LAD +E EC +  W GII R+WP TKY+DVIVTGTMSQYI
Sbjct: 262 EITDRVVRDAVGRVLRADPALADAIEDECARASWEGIIRRLWPRTKYIDVIVTGTMSQYI 321

Query: 326 GTLDYYSNGLPIVCTMYASSECYFGVNLKPMCKPGEVAYTLIPTMAYFEFLPV--NRSKE 385
            TL++Y  GLP+ CTMYASSECYFG+NL PMCKP +VAYTLIPTM Y+EFLPV  N +  
Sbjct: 322 PTLEFYGGGLPLTCTMYASSECYFGLNLNPMCKPSDVAYTLIPTMCYYEFLPVNCNNATA 381

Query: 386 KELEKELVDLVDVKLGHEYELVVTTYSGLYRYRVGDILRVAGFKNNAPQFNFICRKNVAL 445
           +   ++LVDLVDVKLGHEYELVVTTYSGLYRYRVGD+LRVAGFKN AP F+F+ R+NVAL
Sbjct: 382 EASHRDLVDLVDVKLGHEYELVVTTYSGLYRYRVGDVLRVAGFKNKAPMFSFVRRQNVAL 441

Query: 446 SIDSDKTDEVELQNAVKNALTHLIPFDVTLSEYTSCVDTSNIPGHYVLYWELTLNNEIKS 505
           S+DSDKTDE EL  AV  A+ HL PF  +L EYTS  D + IPGHYVL+WEL       S
Sbjct: 442 SVDSDKTDETELHAAVSGAVQHLAPFGASLVEYTSYADAATIPGHYVLFWELRAG----S 501

Query: 506 TPIPPSVFEDCCFAIEESLNAVYRQGRVSDKSIGPLEIKIVETGTFDKLMDFAISLGASI 565
           T +P SVFE+CC ++EE+LN+VYRQGR  D+SIGPLEI++V  GTFDKLMD+AIS GASI
Sbjct: 502 TAVPASVFEECCLSVEEALNSVYRQGRACDRSIGPLEIRVVAEGTFDKLMDYAISRGASI 561

Query: 566 NQYKTPRCVKYEPIIQLLSSRVVSNYFSPKCPKWVP--KQW 602
           NQYK PRCV+  P+++LL +RV   YFSPKCPKW P  KQW
Sbjct: 562 NQYKAPRCVRPGPVVELLDARVQGKYFSPKCPKWSPGNKQW 598

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BLAST of CSPI03G23650 vs. ExPASy Swiss-Prot
Match: Q60EJ6 (Probable indole-3-acetic acid-amido synthetase GH3.4 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.4 PE=2 SV=1)

HSP 1 Score: 792.7 bits (2046), Expect = 2.9e-228
Identity = 392/592 (66.22%), Postives = 461/592 (77.87%), Query Frame = 0 

Query: 23  KNKKTLQFIEDVTANADQVQRRVLTEILSRNAGVEYLRRHGLGGATD--CKTFKNTLPLI 82
           ++++ L+ IE VT NA Q QRRVL EIL++NA  EYLRR G+ G      + F+   PL+
Sbjct: 22  EHREKLEHIERVTRNAGQEQRRVLEEILAQNAQAEYLRRLGVPGDAPGADEAFRRLAPLV 81

Query: 83  SYEDIQPDINRIANGDSSSILCSSPISEFLTSSGTSGGERKLMPTIEEELERRSSLYSLL 142
           +YEDI PD+ RIANGD+S IL   P+SEFLTSSGTSGGERKLMPTIEEE+ERRS LYSLL
Sbjct: 82  TYEDILPDVLRIANGDTSPILSGKPVSEFLTSSGTSGGERKLMPTIEEEMERRSGLYSLL 141

Query: 143 MPVMSQFIEGLEKGKGMYFLFIKSESKTPSGLLARPVLTSYYKSPHFKNRPFDPYTNYTS 202
           MPVMS+ + GL+KGK MY  F+KSE +TP GL ARPVLTS+Y+S +F  RP DPYT YTS
Sbjct: 142 MPVMSRQVPGLDKGKAMYLYFVKSEWRTPGGLPARPVLTSFYRSRYFLERPHDPYTVYTS 201

Query: 203 PNEAILCSDSYQSMYAQLLCGLLHRTDVLRVGAVFASGFIRAIRFLEKHYTLLCHDIRTG 262
           P+EA+LC D+YQSMYAQL+CGL+HR DVLRVGAVFASGF+RAIRFLEKH+  LC DIR G
Sbjct: 202 PDEAVLCEDAYQSMYAQLICGLVHRADVLRVGAVFASGFLRAIRFLEKHWPSLCRDIRAG 261

Query: 263 TLNSEITDQGIRESVRKILK-ADGKLADFVEGECGKKCWGGIISRIWPNTKYVDVIVTGT 322
            L+  +TD  +R +V ++L+ AD  LAD +E EC +  W GII R+WP+TKY+DVIVTG 
Sbjct: 262 ELDGGVTDPAVRGAVGRVLRGADPALADAIEAECARPSWQGIIRRVWPSTKYIDVIVTGA 321

Query: 323 MSQYIGTLDYYSNGLPIVCTMYASSECYFGVNLKPMCKPGEVAYTLIPTMAYFEFLPVNR 382
           M+QYI TL++Y  GLP+ CTMYASSECYFG+NL PMCKP EVAYTLIPTM YFEFLPVN 
Sbjct: 322 MAQYIPTLEFYGGGLPLACTMYASSECYFGLNLNPMCKPSEVAYTLIPTMCYFEFLPVNS 381

Query: 383 SKEKELEKE-----LVDLVDVKLGHEYELVVTTYSGLYRYRVGDILRVAGFKNNAPQFNF 442
                   E     LVDLVDVKLGHEYELVVTTYSGLYRYRVGD+LRVAGFKN AP F F
Sbjct: 382 GANDVAAPEPDHRGLVDLVDVKLGHEYELVVTTYSGLYRYRVGDVLRVAGFKNAAPMFAF 441

Query: 443 ICRKNVALSIDSDKTDEVELQNAVKNALTHLIPFDVTLSEYTSCVDT-SNIPGHYVLYWE 502
           + RKNVALSIDSDKTDE EL  AV  A+ HL PF  +L EYTS  DT + IPGHYVL+WE
Sbjct: 442 VRRKNVALSIDSDKTDEAELHAAVTEAVQHLAPFGASLVEYTSYADTATTIPGHYVLFWE 501

Query: 503 LTLNNEIKSTPIPPSVFEDCCFAIEESLNAVYRQGRVSDKSIGPLEIKIVETGTFDKLMD 562
             L +    TP+P SVFEDCC A+EE LN+VYRQ R +D+SIGPLEI++V  GTFDKLMD
Sbjct: 502 --LRSPAGGTPVPASVFEDCCLAVEEGLNSVYRQCRAADRSIGPLEIRVVADGTFDKLMD 561

Query: 563 FAISLGASINQYKTPRCVKYEPIIQLLSSRVVSNYFSPKCPKWVP---KQWI 603
           +A+S GASINQYK PRCV+  P+++LL  RV + YFSPKCPKW     KQWI
Sbjct: 562 YALSRGASINQYKAPRCVRPGPVVELLDGRVQATYFSPKCPKWCAGGNKQWI 611

[Close]
BLAST of CSPI03G23650 vs. ExPASy Swiss-Prot
Match: O82333 (Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Arabidopsis thaliana OX=3702 GN=GH3.1 PE=2 SV=1)

HSP 1 Score: 760.4 bits (1962), Expect = 1.6e-218
Identity = 375/580 (64.66%), Postives = 450/580 (77.59%), Query Frame = 0 

Query: 22  EKNKKTLQFIEDVTANADQVQRRVLTEILSRNAGVEYLRRHGLGGATDCKTFKNTLPLIS 81
           EK+ K L+FIE++T NAD VQ  +L EIL+RNA  EYLRR  L GATD  TFK  +P+I+
Sbjct: 17  EKDAKALRFIEEMTRNADTVQENLLAEILARNADTEYLRRFNLCGATDRDTFKTKIPVIT 76

Query: 82  YEDIQPDINRIANGDSSSILCSSPISEFLTSSGTSGGERKLMPTIEEELERRSSLYSLLM 141
           YED+QP+I RIA+GD S IL + PISEFLTSSGTS GERKLMPTI+EEL+RR  LYSLLM
Sbjct: 77  YEDLQPEIQRIADGDRSPILSAHPISEFLTSSGTSAGERKLMPTIKEELDRRQLLYSLLM 136

Query: 142 PVMSQFIEGLEKGKGMYFLFIKSESKTPSGLLARPVLTSYYKSPHFKNRPFDPYTNYTSP 201
           PVM+ ++ GL+KGKGMYFLF+KSE+KTP GL ARPVLTSYYKS HF++RP+DPY  YTSP
Sbjct: 137 PVMNLYVPGLDKGKGMYFLFVKSETKTPGGLPARPVLTSYYKSEHFRSRPYDPYNVYTSP 196

Query: 202 NEAILCSDSYQSMYAQLLCGLLHRTDVLRVGAVFASGFIRAIRFLEKHYTLLCHDIRTGT 261
           NEAILC DS+QSMY Q+LCGLL R  VLRVGAVFASG +RAIRFL+ H++   HDI  G 
Sbjct: 197 NEAILCPDSFQSMYTQMLCGLLDRLSVLRVGAVFASGLLRAIRFLQLHWSRFAHDIELGC 256

Query: 262 LNSEITDQGIRESVRKILKADGKLADFVEGECGKKCWGGIISRIWPNTKYVDVIVTGTMS 321
           L+SEITD  IR+ +  ILK D  LA+F+  EC    W  II+RIWPNTKY+DVIVTG M+
Sbjct: 257 LDSEITDPSIRQCMSGILKPDPVLAEFIRRECKSDNWEKIITRIWPNTKYLDVIVTGAMA 316

Query: 322 QYIGTLDYYSNGLPIVCTMYASSECYFGVNLKPMCKPGEVAYTLIPTMAYFEFLPVNRSK 381
           QYI TL+YYS GLP+ CTMYASSECYFG+NL PM KP EV+YT++P MAYFEF+P+  +K
Sbjct: 317 QYIPTLEYYSGGLPMACTMYASSECYFGLNLNPMSKPSEVSYTIMPNMAYFEFIPLGGTK 376

Query: 382 EKELEKELVDLVDVKLGHEYELVVTTYSGLYRYRVGDILRVAGFKNNAPQFNFICRKNVA 441
                   V+LVDV +G EYELVVTTY+GL RYRVGDILRV GF N+APQF+F+ RKNV 
Sbjct: 377 -------AVELVDVNIGKEYELVVTTYAGLCRYRVGDILRVTGFHNSAPQFHFVRRKNVL 436

Query: 442 LSIDSDKTDEVELQNAVKNALTHL-IPFDVTLSEYTSCVDTSNIPGHYVLYWELTLNNEI 501
           LSIDSDKTDE ELQ AV+NA + L       ++EYTS  DTS IPGHYVLYWEL + +  
Sbjct: 437 LSIDSDKTDESELQKAVENASSILHEECGSRVAEYTSYADTSTIPGHYVLYWELLVRDGA 496

Query: 502 KSTPIPPSVFEDCCFAIEESLNAVYRQGRVSDKSIGPLEIKIVETGTFDKLMDFAISLGA 561
           +           CC  +EESLN+VYRQ RV+D S+GPLEI++V  GTF++LMD+AIS GA
Sbjct: 497 RQP--SHETLTRCCLGMEESLNSVYRQSRVADNSVGPLEIRVVRNGTFEELMDYAISRGA 556

Query: 562 SINQYKTPRCVKYEPIIQLLSSRVVSNYFSPKCPKWVPKQ 601
           SINQYK PRCV + PI++LL SRVVS +FSP  P W P++
Sbjct: 557 SINQYKVPRCVNFTPIVELLDSRVVSAHFSPSLPHWTPER 587

[Close]
BLAST of CSPI03G23650 vs. ExPASy TrEMBL
Match: A0A0A0LAR6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G431430 PE=3 SV=1)

HSP 1 Score: 1225.3 bits (3169), Expect = 0.0e+00
Identity = 602/602 (100.00%), Postives = 602/602 (100.00%), Query Frame = 0 

Query: 1   MPEETPQNSSFLSTTEYYKLLEKNKKTLQFIEDVTANADQVQRRVLTEILSRNAGVEYLR 60
           MPEETPQNSSFLSTTEYYKLLEKNKKTLQFIEDVTANADQVQRRVLTEILSRNAGVEYLR
Sbjct: 1   MPEETPQNSSFLSTTEYYKLLEKNKKTLQFIEDVTANADQVQRRVLTEILSRNAGVEYLR 60

Query: 61  RHGLGGATDCKTFKNTLPLISYEDIQPDINRIANGDSSSILCSSPISEFLTSSGTSGGER 120
           RHGLGGATDCKTFKNTLPLISYEDIQPDINRIANGDSSSILCSSPISEFLTSSGTSGGER
Sbjct: 61  RHGLGGATDCKTFKNTLPLISYEDIQPDINRIANGDSSSILCSSPISEFLTSSGTSGGER 120

Query: 121 KLMPTIEEELERRSSLYSLLMPVMSQFIEGLEKGKGMYFLFIKSESKTPSGLLARPVLTS 180
           KLMPTIEEELERRSSLYSLLMPVMSQFIEGLEKGKGMYFLFIKSESKTPSGLLARPVLTS
Sbjct: 121 KLMPTIEEELERRSSLYSLLMPVMSQFIEGLEKGKGMYFLFIKSESKTPSGLLARPVLTS 180

Query: 181 YYKSPHFKNRPFDPYTNYTSPNEAILCSDSYQSMYAQLLCGLLHRTDVLRVGAVFASGFI 240
           YYKSPHFKNRPFDPYTNYTSPNEAILCSDSYQSMYAQLLCGLLHRTDVLRVGAVFASGFI
Sbjct: 181 YYKSPHFKNRPFDPYTNYTSPNEAILCSDSYQSMYAQLLCGLLHRTDVLRVGAVFASGFI 240

Query: 241 RAIRFLEKHYTLLCHDIRTGTLNSEITDQGIRESVRKILKADGKLADFVEGECGKKCWGG 300
           RAIRFLEKHYTLLCHDIRTGTLNSEITDQGIRESVRKILKADGKLADFVEGECGKKCWGG
Sbjct: 241 RAIRFLEKHYTLLCHDIRTGTLNSEITDQGIRESVRKILKADGKLADFVEGECGKKCWGG 300

Query: 301 IISRIWPNTKYVDVIVTGTMSQYIGTLDYYSNGLPIVCTMYASSECYFGVNLKPMCKPGE 360
           IISRIWPNTKYVDVIVTGTMSQYIGTLDYYSNGLPIVCTMYASSECYFGVNLKPMCKPGE
Sbjct: 301 IISRIWPNTKYVDVIVTGTMSQYIGTLDYYSNGLPIVCTMYASSECYFGVNLKPMCKPGE 360

Query: 361 VAYTLIPTMAYFEFLPVNRSKEKELEKELVDLVDVKLGHEYELVVTTYSGLYRYRVGDIL 420
           VAYTLIPTMAYFEFLPVNRSKEKELEKELVDLVDVKLGHEYELVVTTYSGLYRYRVGDIL
Sbjct: 361 VAYTLIPTMAYFEFLPVNRSKEKELEKELVDLVDVKLGHEYELVVTTYSGLYRYRVGDIL 420

Query: 421 RVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNALTHLIPFDVTLSEYTSCVD 480
           RVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNALTHLIPFDVTLSEYTSCVD
Sbjct: 421 RVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNALTHLIPFDVTLSEYTSCVD 480

Query: 481 TSNIPGHYVLYWELTLNNEIKSTPIPPSVFEDCCFAIEESLNAVYRQGRVSDKSIGPLEI 540
           TSNIPGHYVLYWELTLNNEIKSTPIPPSVFEDCCFAIEESLNAVYRQGRVSDKSIGPLEI
Sbjct: 481 TSNIPGHYVLYWELTLNNEIKSTPIPPSVFEDCCFAIEESLNAVYRQGRVSDKSIGPLEI 540

Query: 541 KIVETGTFDKLMDFAISLGASINQYKTPRCVKYEPIIQLLSSRVVSNYFSPKCPKWVPKQ 600
           KIVETGTFDKLMDFAISLGASINQYKTPRCVKYEPIIQLLSSRVVSNYFSPKCPKWVPKQ
Sbjct: 541 KIVETGTFDKLMDFAISLGASINQYKTPRCVKYEPIIQLLSSRVVSNYFSPKCPKWVPKQ 600

Query: 601 WI 603
           WI
Sbjct: 601 WI 602

[Close]
BLAST of CSPI03G23650 vs. ExPASy TrEMBL
Match: A0A5A7V8A5 (Indole-3-acetic acid-amido synthetase GH3.6 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold977G00060 PE=3 SV=1)

HSP 1 Score: 1207.6 bits (3123), Expect = 0.0e+00
Identity = 592/601 (98.50%), Postives = 595/601 (99.00%), Query Frame = 0 

Query: 1   MPEETPQNSSFLSTTEYYKLLEKNKKTLQFIEDVTANADQVQRRVLTEILSRNAGVEYLR 60
           MPEETPQ SSFLSTTEYYKLLEKNKKTLQFIEDVT NADQVQRRVLTEILSRNAGVEYLR
Sbjct: 1   MPEETPQKSSFLSTTEYYKLLEKNKKTLQFIEDVTTNADQVQRRVLTEILSRNAGVEYLR 60

Query: 61  RHGLGGATDCKTFKNTLPLISYEDIQPDINRIANGDSSSILCSSPISEFLTSSGTSGGER 120
           RHGLGGATDCKTFKNTLPLISYEDIQPDINRIANGD+S ILCSSPISEFLTSSGTSGGER
Sbjct: 61  RHGLGGATDCKTFKNTLPLISYEDIQPDINRIANGDTSPILCSSPISEFLTSSGTSGGER 120

Query: 121 KLMPTIEEELERRSSLYSLLMPVMSQFIEGLEKGKGMYFLFIKSESKTPSGLLARPVLTS 180
           KLMPTIEEELERRSSLYSLLMPVMSQFIEGLEKGKGMYFLFIKSESKTPSGLLARPVLTS
Sbjct: 121 KLMPTIEEELERRSSLYSLLMPVMSQFIEGLEKGKGMYFLFIKSESKTPSGLLARPVLTS 180

Query: 181 YYKSPHFKNRPFDPYTNYTSPNEAILCSDSYQSMYAQLLCGLLHRTDVLRVGAVFASGFI 240
           YYKSPHFKNRPFDPYTNYTSPNEAILCSDSYQSMYAQLLCGLLHRTDVLRVGAVFASGFI
Sbjct: 181 YYKSPHFKNRPFDPYTNYTSPNEAILCSDSYQSMYAQLLCGLLHRTDVLRVGAVFASGFI 240

Query: 241 RAIRFLEKHYTLLCHDIRTGTLNSEITDQGIRESVRKILKADGKLADFVEGECGKKCWGG 300
           RAIRFLEKHYTLLCHDIRTGTLNSEITDQGIRESVRKILKADGKLADFVEGECGKKCWGG
Sbjct: 241 RAIRFLEKHYTLLCHDIRTGTLNSEITDQGIRESVRKILKADGKLADFVEGECGKKCWGG 300

Query: 301 IISRIWPNTKYVDVIVTGTMSQYIGTLDYYSNGLPIVCTMYASSECYFGVNLKPMCKPGE 360
           IISRIWPNTKYVDVIVTGTMSQYIGTLDYYSNGLPIVCTMYASSECYFGVNLKPMCKPGE
Sbjct: 301 IISRIWPNTKYVDVIVTGTMSQYIGTLDYYSNGLPIVCTMYASSECYFGVNLKPMCKPGE 360

Query: 361 VAYTLIPTMAYFEFLPVNRSKEKELEKELVDLVDVKLGHEYELVVTTYSGLYRYRVGDIL 420
           VAYTLIPTMAYFEFLPVNRSKEKELEKELVDLVDVKLGHEYELVVTTYSGLYRYRVGDIL
Sbjct: 361 VAYTLIPTMAYFEFLPVNRSKEKELEKELVDLVDVKLGHEYELVVTTYSGLYRYRVGDIL 420

Query: 421 RVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNALTHLIPFDVTLSEYTSCVD 480
           RVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNALTHLIPFDVTLSEYTSCVD
Sbjct: 421 RVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNALTHLIPFDVTLSEYTSCVD 480

Query: 481 TSNIPGHYVLYWELTLNNEIKSTPIPPSVFEDCCFAIEESLNAVYRQGRVSDKSIGPLEI 540
           TS IPGHYVLYWELT NNEIKSTPIPPSVFEDCCFAIEESLNAVYRQGRVSDKSIGPLEI
Sbjct: 481 TSKIPGHYVLYWELTPNNEIKSTPIPPSVFEDCCFAIEESLNAVYRQGRVSDKSIGPLEI 540

Query: 541 KIVETGTFDKLMDFAISLGASINQYKTPRCVKYEPIIQLLSSRVVSNYFSPKCPKWVPKQ 600
           KIVETGTFDKLMD+AISLGASINQYKTPRCVKYEPIIQLL+SR VSNYFSPKCPKWVPKQ
Sbjct: 541 KIVETGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRTVSNYFSPKCPKWVPKQ 600

Query: 601 W 602
           W
Sbjct: 601 W 601

[Close]
BLAST of CSPI03G23650 vs. ExPASy TrEMBL
Match: A0A1S3BYK9 (indole-3-acetic acid-amido synthetase GH3.6 OS=Cucumis melo OX=3656 GN=LOC103494980 PE=3 SV=1)

HSP 1 Score: 1207.6 bits (3123), Expect = 0.0e+00
Identity = 592/601 (98.50%), Postives = 595/601 (99.00%), Query Frame = 0 

Query: 1   MPEETPQNSSFLSTTEYYKLLEKNKKTLQFIEDVTANADQVQRRVLTEILSRNAGVEYLR 60
           MPEETPQ SSFLSTTEYYKLLEKNKKTLQFIEDVT NADQVQRRVLTEILSRNAGVEYLR
Sbjct: 1   MPEETPQKSSFLSTTEYYKLLEKNKKTLQFIEDVTTNADQVQRRVLTEILSRNAGVEYLR 60

Query: 61  RHGLGGATDCKTFKNTLPLISYEDIQPDINRIANGDSSSILCSSPISEFLTSSGTSGGER 120
           RHGLGGATDCKTFKNTLPLISYEDIQPDINRIANGD+S ILCSSPISEFLTSSGTSGGER
Sbjct: 61  RHGLGGATDCKTFKNTLPLISYEDIQPDINRIANGDTSPILCSSPISEFLTSSGTSGGER 120

Query: 121 KLMPTIEEELERRSSLYSLLMPVMSQFIEGLEKGKGMYFLFIKSESKTPSGLLARPVLTS 180
           KLMPTIEEELERRSSLYSLLMPVMSQFIEGLEKGKGMYFLFIKSESKTPSGLLARPVLTS
Sbjct: 121 KLMPTIEEELERRSSLYSLLMPVMSQFIEGLEKGKGMYFLFIKSESKTPSGLLARPVLTS 180

Query: 181 YYKSPHFKNRPFDPYTNYTSPNEAILCSDSYQSMYAQLLCGLLHRTDVLRVGAVFASGFI 240
           YYKSPHFKNRPFDPYTNYTSPNEAILCSDSYQSMYAQLLCGLLHRTDVLRVGAVFASGFI
Sbjct: 181 YYKSPHFKNRPFDPYTNYTSPNEAILCSDSYQSMYAQLLCGLLHRTDVLRVGAVFASGFI 240

Query: 241 RAIRFLEKHYTLLCHDIRTGTLNSEITDQGIRESVRKILKADGKLADFVEGECGKKCWGG 300
           RAIRFLEKHYTLLCHDIRTGTLNSEITDQGIRESVRKILKADGKLADFVEGECGKKCWGG
Sbjct: 241 RAIRFLEKHYTLLCHDIRTGTLNSEITDQGIRESVRKILKADGKLADFVEGECGKKCWGG 300

Query: 301 IISRIWPNTKYVDVIVTGTMSQYIGTLDYYSNGLPIVCTMYASSECYFGVNLKPMCKPGE 360
           IISRIWPNTKYVDVIVTGTMSQYIGTLDYYSNGLPIVCTMYASSECYFGVNLKPMCKPGE
Sbjct: 301 IISRIWPNTKYVDVIVTGTMSQYIGTLDYYSNGLPIVCTMYASSECYFGVNLKPMCKPGE 360

Query: 361 VAYTLIPTMAYFEFLPVNRSKEKELEKELVDLVDVKLGHEYELVVTTYSGLYRYRVGDIL 420
           VAYTLIPTMAYFEFLPVNRSKEKELEKELVDLVDVKLGHEYELVVTTYSGLYRYRVGDIL
Sbjct: 361 VAYTLIPTMAYFEFLPVNRSKEKELEKELVDLVDVKLGHEYELVVTTYSGLYRYRVGDIL 420

Query: 421 RVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNALTHLIPFDVTLSEYTSCVD 480
           RVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNALTHLIPFDVTLSEYTSCVD
Sbjct: 421 RVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNALTHLIPFDVTLSEYTSCVD 480

Query: 481 TSNIPGHYVLYWELTLNNEIKSTPIPPSVFEDCCFAIEESLNAVYRQGRVSDKSIGPLEI 540
           TS IPGHYVLYWELT NNEIKSTPIPPSVFEDCCFAIEESLNAVYRQGRVSDKSIGPLEI
Sbjct: 481 TSKIPGHYVLYWELTPNNEIKSTPIPPSVFEDCCFAIEESLNAVYRQGRVSDKSIGPLEI 540

Query: 541 KIVETGTFDKLMDFAISLGASINQYKTPRCVKYEPIIQLLSSRVVSNYFSPKCPKWVPKQ 600
           KIVETGTFDKLMD+AISLGASINQYKTPRCVKYEPIIQLL+SR VSNYFSPKCPKWVPKQ
Sbjct: 541 KIVETGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRTVSNYFSPKCPKWVPKQ 600

Query: 601 W 602
           W
Sbjct: 601 W 601

[Close]
BLAST of CSPI03G23650 vs. ExPASy TrEMBL
Match: A0A6J1F6T2 (indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita moschata OX=3662 GN=LOC111442645 PE=3 SV=1)

HSP 1 Score: 1082.8 bits (2799), Expect = 0.0e+00
Identity = 535/606 (88.28%), Postives = 564/606 (93.07%), Query Frame = 0 

Query: 1   MPEETPQNSSFLSTTEYYKLLEKNKKTLQFIEDVTANADQVQRRVLTEILSRNAGVEYLR 60
           MPEE PQ SSFLSTTEYYKLL++NKKTLQFIEDVTANA QVQRRVL EILSRNA VEYLR
Sbjct: 1   MPEEAPQKSSFLSTTEYYKLLQRNKKTLQFIEDVTANAAQVQRRVLAEILSRNAHVEYLR 60

Query: 61  RHGLGGATDCKTFKNTLPLISYEDIQPDINRIANGDSSSILCSSPISEFLTSSGTSGGER 120
           RHGL GATD KTFK T+P+ISYEDIQ DINRIANGDSS ILCSSPISEFLTSSGTSGGER
Sbjct: 61  RHGLAGATDPKTFKKTIPVISYEDIQHDINRIANGDSSPILCSSPISEFLTSSGTSGGER 120

Query: 121 KLMPTIEEELERRSSLYSLLMPVMSQFIEGLEKGKGMYFLFIKSESKTPSGLLARPVLTS 180
           KLMPTIEEELERRS LYSLLMPVMSQF+ GLEKGKGMYFLFIKSESKTPSGLLARPVLTS
Sbjct: 121 KLMPTIEEELERRSLLYSLLMPVMSQFMGGLEKGKGMYFLFIKSESKTPSGLLARPVLTS 180

Query: 181 YYKSPHFKNRPFDPYTNYTSPNEAILCSDSYQSMYAQLLCGLLHRTDVLRVGAVFASGFI 240
           YYKS HFK+RPFDPYTNYTSPNEAILCSD+YQSMYAQLLCGL HRTDVLRVGAVFASGFI
Sbjct: 181 YYKSAHFKHRPFDPYTNYTSPNEAILCSDAYQSMYAQLLCGLHHRTDVLRVGAVFASGFI 240

Query: 241 RAIRFLEKHYTLLCHDIRTGTLNSEITDQGIRESVRKILKADGKLADFVEGECGKKCWGG 300
           RAIRFLEKH+ LLCHDIRTGTLN +ITDQ IRES++K LK D KLADF+EGEC KK W G
Sbjct: 241 RAIRFLEKHFHLLCHDIRTGTLNPQITDQPIRESLKKTLKPDPKLADFIEGECSKKSWHG 300

Query: 301 IISRIWPNTKYVDVIVTGTMSQYIGTLDYYSNGLPIVCTMYASSECYFGVNLKPMCKPGE 360
           II+RIWPNTKYVDVIVTGTMSQYI TLDYYSNGLP+VCTMYASSECYFGVNL P+CKP E
Sbjct: 301 IITRIWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPICKPSE 360

Query: 361 VAYTLIPTMAYFEFLPVNRS---KEKELEKELVDLVDVKLGHEYELVVTTYSGLYRYRVG 420
           V+YTLIPTMAYFEFLPVNR+    EKEL+ ELVDLVDV+LG EYELVVTTY+GLYRYRVG
Sbjct: 361 VSYTLIPTMAYFEFLPVNRNMSLTEKELQLELVDLVDVQLGQEYELVVTTYAGLYRYRVG 420

Query: 421 DILRVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNALTHLIPFDVTLSEYTS 480
           D+LRVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNAL+HL+PFD T+SEYTS
Sbjct: 421 DVLRVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNALSHLVPFDATVSEYTS 480

Query: 481 CVDTSNIPGHYVLYWELTLNNEIKSTPIPPSVFEDCCFAIEESLNAVYRQGRVSDKSIGP 540
           C DTS IPGHYVLYWEL++N   K+TPIPPSVFEDCC  IEESLNAVYRQGRVSDKSIGP
Sbjct: 481 CADTSKIPGHYVLYWELSVNG-AKTTPIPPSVFEDCCLTIEESLNAVYRQGRVSDKSIGP 540

Query: 541 LEIKIVETGTFDKLMDFAISLGASINQYKTPRCVKYEPIIQLLSSRVVSNYFSPKCPKWV 600
           LEIKIVETGTFDKLMD+AISLGASINQYKTPRCVKYEPIIQLL+SRVVSNYFSPKCPKWV
Sbjct: 541 LEIKIVETGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPKCPKWV 600

Query: 601 P--KQW 602
           P  KQW
Sbjct: 601 PGHKQW 605

[Close]
BLAST of CSPI03G23650 vs. ExPASy TrEMBL
Match: A0A6J1IM75 (indole-3-acetic acid-amido synthetase GH3.6 OS=Cucurbita maxima OX=3661 GN=LOC111476851 PE=3 SV=1)

HSP 1 Score: 1077.8 bits (2786), Expect = 0.0e+00
Identity = 532/606 (87.79%), Postives = 562/606 (92.74%), Query Frame = 0 

Query: 1   MPEETPQNSSFLSTTEYYKLLEKNKKTLQFIEDVTANADQVQRRVLTEILSRNAGVEYLR 60
           MPEE PQ SSFLSTTEYYKLL++NKKTLQFIEDVTANA Q+QRRVL EILSRNA VEYLR
Sbjct: 1   MPEEAPQKSSFLSTTEYYKLLQRNKKTLQFIEDVTANAAQIQRRVLAEILSRNAHVEYLR 60

Query: 61  RHGLGGATDCKTFKNTLPLISYEDIQPDINRIANGDSSSILCSSPISEFLTSSGTSGGER 120
           RHGL GATD KTFKNT+P+ISYEDIQ DINRIANGD S ILCSSPISEFLTSSGTSGGER
Sbjct: 61  RHGLAGATDSKTFKNTIPVISYEDIQHDINRIANGDFSPILCSSPISEFLTSSGTSGGER 120

Query: 121 KLMPTIEEELERRSSLYSLLMPVMSQFIEGLEKGKGMYFLFIKSESKTPSGLLARPVLTS 180
           KLMPTIEEELERRS LYSLLMPVMSQF+ GLEKGKGMYFLFIKSESKTPSGLLARPVLTS
Sbjct: 121 KLMPTIEEELERRSLLYSLLMPVMSQFMGGLEKGKGMYFLFIKSESKTPSGLLARPVLTS 180

Query: 181 YYKSPHFKNRPFDPYTNYTSPNEAILCSDSYQSMYAQLLCGLLHRTDVLRVGAVFASGFI 240
           YYKS HFK+RPFDPYTNYTSPNEAILCSD+YQSMYAQLLCGL HRTDVLRVGAVFASGFI
Sbjct: 181 YYKSAHFKHRPFDPYTNYTSPNEAILCSDAYQSMYAQLLCGLHHRTDVLRVGAVFASGFI 240

Query: 241 RAIRFLEKHYTLLCHDIRTGTLNSEITDQGIRESVRKILKADGKLADFVEGECGKKCWGG 300
           RAIRFLEKH+ LLCHDIRTGTLN +ITDQ IRES++K LK D KLADF+EGEC KK W G
Sbjct: 241 RAIRFLEKHFHLLCHDIRTGTLNPQITDQPIRESLKKTLKPDPKLADFIEGECSKKSWHG 300

Query: 301 IISRIWPNTKYVDVIVTGTMSQYIGTLDYYSNGLPIVCTMYASSECYFGVNLKPMCKPGE 360
           II+RIWPNTKYVDVIVTGTMSQYI TLDYYSNGLP+VCTMYASSECYFGVNL P+CKP E
Sbjct: 301 IITRIWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPICKPAE 360

Query: 361 VAYTLIPTMAYFEFLPVNRS---KEKELEKELVDLVDVKLGHEYELVVTTYSGLYRYRVG 420
           V+YTLIPTMAYFEFLPVNR+    EKEL+ ELVDLVDV+LG EYELVVTTY+GLYRYRVG
Sbjct: 361 VSYTLIPTMAYFEFLPVNRNMSLTEKELQLELVDLVDVQLGQEYELVVTTYAGLYRYRVG 420

Query: 421 DILRVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNALTHLIPFDVTLSEYTS 480
           D+LRVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNAL+HL+PFD T+SEYTS
Sbjct: 421 DVLRVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNALSHLVPFDATVSEYTS 480

Query: 481 CVDTSNIPGHYVLYWELTLNNEIKSTPIPPSVFEDCCFAIEESLNAVYRQGRVSDKSIGP 540
           C DTS IPGHYVLYWEL++N   K+T IPPSVFEDCC  IEESLNAVYRQGRVSDKSIGP
Sbjct: 481 CADTSKIPGHYVLYWELSVNG-AKTTLIPPSVFEDCCLTIEESLNAVYRQGRVSDKSIGP 540

Query: 541 LEIKIVETGTFDKLMDFAISLGASINQYKTPRCVKYEPIIQLLSSRVVSNYFSPKCPKWV 600
           LEIKIVETGTFDKLMD+AISLGASINQYKTPRCVKYEPIIQLL+SR VSNYFSPKCPKWV
Sbjct: 541 LEIKIVETGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRAVSNYFSPKCPKWV 600

Query: 601 P--KQW 602
           P  KQW
Sbjct: 601 PGHKQW 605

[Close]
BLAST of CSPI03G23650 vs. NCBI nr
Match: XP_004149186.1 (indole-3-acetic acid-amido synthetase GH3.6 [Cucumis sativus] >KGN58009.1 hypothetical protein Csa_011220 [Cucumis sativus])

HSP 1 Score: 1225.3 bits (3169), Expect = 0.0e+00
Identity = 602/602 (100.00%), Postives = 602/602 (100.00%), Query Frame = 0 

Query: 1   MPEETPQNSSFLSTTEYYKLLEKNKKTLQFIEDVTANADQVQRRVLTEILSRNAGVEYLR 60
           MPEETPQNSSFLSTTEYYKLLEKNKKTLQFIEDVTANADQVQRRVLTEILSRNAGVEYLR
Sbjct: 1   MPEETPQNSSFLSTTEYYKLLEKNKKTLQFIEDVTANADQVQRRVLTEILSRNAGVEYLR 60

Query: 61  RHGLGGATDCKTFKNTLPLISYEDIQPDINRIANGDSSSILCSSPISEFLTSSGTSGGER 120
           RHGLGGATDCKTFKNTLPLISYEDIQPDINRIANGDSSSILCSSPISEFLTSSGTSGGER
Sbjct: 61  RHGLGGATDCKTFKNTLPLISYEDIQPDINRIANGDSSSILCSSPISEFLTSSGTSGGER 120

Query: 121 KLMPTIEEELERRSSLYSLLMPVMSQFIEGLEKGKGMYFLFIKSESKTPSGLLARPVLTS 180
           KLMPTIEEELERRSSLYSLLMPVMSQFIEGLEKGKGMYFLFIKSESKTPSGLLARPVLTS
Sbjct: 121 KLMPTIEEELERRSSLYSLLMPVMSQFIEGLEKGKGMYFLFIKSESKTPSGLLARPVLTS 180

Query: 181 YYKSPHFKNRPFDPYTNYTSPNEAILCSDSYQSMYAQLLCGLLHRTDVLRVGAVFASGFI 240
           YYKSPHFKNRPFDPYTNYTSPNEAILCSDSYQSMYAQLLCGLLHRTDVLRVGAVFASGFI
Sbjct: 181 YYKSPHFKNRPFDPYTNYTSPNEAILCSDSYQSMYAQLLCGLLHRTDVLRVGAVFASGFI 240

Query: 241 RAIRFLEKHYTLLCHDIRTGTLNSEITDQGIRESVRKILKADGKLADFVEGECGKKCWGG 300
           RAIRFLEKHYTLLCHDIRTGTLNSEITDQGIRESVRKILKADGKLADFVEGECGKKCWGG
Sbjct: 241 RAIRFLEKHYTLLCHDIRTGTLNSEITDQGIRESVRKILKADGKLADFVEGECGKKCWGG 300

Query: 301 IISRIWPNTKYVDVIVTGTMSQYIGTLDYYSNGLPIVCTMYASSECYFGVNLKPMCKPGE 360
           IISRIWPNTKYVDVIVTGTMSQYIGTLDYYSNGLPIVCTMYASSECYFGVNLKPMCKPGE
Sbjct: 301 IISRIWPNTKYVDVIVTGTMSQYIGTLDYYSNGLPIVCTMYASSECYFGVNLKPMCKPGE 360

Query: 361 VAYTLIPTMAYFEFLPVNRSKEKELEKELVDLVDVKLGHEYELVVTTYSGLYRYRVGDIL 420
           VAYTLIPTMAYFEFLPVNRSKEKELEKELVDLVDVKLGHEYELVVTTYSGLYRYRVGDIL
Sbjct: 361 VAYTLIPTMAYFEFLPVNRSKEKELEKELVDLVDVKLGHEYELVVTTYSGLYRYRVGDIL 420

Query: 421 RVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNALTHLIPFDVTLSEYTSCVD 480
           RVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNALTHLIPFDVTLSEYTSCVD
Sbjct: 421 RVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNALTHLIPFDVTLSEYTSCVD 480

Query: 481 TSNIPGHYVLYWELTLNNEIKSTPIPPSVFEDCCFAIEESLNAVYRQGRVSDKSIGPLEI 540
           TSNIPGHYVLYWELTLNNEIKSTPIPPSVFEDCCFAIEESLNAVYRQGRVSDKSIGPLEI
Sbjct: 481 TSNIPGHYVLYWELTLNNEIKSTPIPPSVFEDCCFAIEESLNAVYRQGRVSDKSIGPLEI 540

Query: 541 KIVETGTFDKLMDFAISLGASINQYKTPRCVKYEPIIQLLSSRVVSNYFSPKCPKWVPKQ 600
           KIVETGTFDKLMDFAISLGASINQYKTPRCVKYEPIIQLLSSRVVSNYFSPKCPKWVPKQ
Sbjct: 541 KIVETGTFDKLMDFAISLGASINQYKTPRCVKYEPIIQLLSSRVVSNYFSPKCPKWVPKQ 600

Query: 601 WI 603
           WI
Sbjct: 601 WI 602

[Close]
BLAST of CSPI03G23650 vs. NCBI nr
Match: XP_008454612.1 (PREDICTED: indole-3-acetic acid-amido synthetase GH3.6 [Cucumis melo] >KAA0063434.1 indole-3-acetic acid-amido synthetase GH3.6 [Cucumis melo var. makuwa])

HSP 1 Score: 1207.6 bits (3123), Expect = 0.0e+00
Identity = 592/601 (98.50%), Postives = 595/601 (99.00%), Query Frame = 0 

Query: 1   MPEETPQNSSFLSTTEYYKLLEKNKKTLQFIEDVTANADQVQRRVLTEILSRNAGVEYLR 60
           MPEETPQ SSFLSTTEYYKLLEKNKKTLQFIEDVT NADQVQRRVLTEILSRNAGVEYLR
Sbjct: 1   MPEETPQKSSFLSTTEYYKLLEKNKKTLQFIEDVTTNADQVQRRVLTEILSRNAGVEYLR 60

Query: 61  RHGLGGATDCKTFKNTLPLISYEDIQPDINRIANGDSSSILCSSPISEFLTSSGTSGGER 120
           RHGLGGATDCKTFKNTLPLISYEDIQPDINRIANGD+S ILCSSPISEFLTSSGTSGGER
Sbjct: 61  RHGLGGATDCKTFKNTLPLISYEDIQPDINRIANGDTSPILCSSPISEFLTSSGTSGGER 120

Query: 121 KLMPTIEEELERRSSLYSLLMPVMSQFIEGLEKGKGMYFLFIKSESKTPSGLLARPVLTS 180
           KLMPTIEEELERRSSLYSLLMPVMSQFIEGLEKGKGMYFLFIKSESKTPSGLLARPVLTS
Sbjct: 121 KLMPTIEEELERRSSLYSLLMPVMSQFIEGLEKGKGMYFLFIKSESKTPSGLLARPVLTS 180

Query: 181 YYKSPHFKNRPFDPYTNYTSPNEAILCSDSYQSMYAQLLCGLLHRTDVLRVGAVFASGFI 240
           YYKSPHFKNRPFDPYTNYTSPNEAILCSDSYQSMYAQLLCGLLHRTDVLRVGAVFASGFI
Sbjct: 181 YYKSPHFKNRPFDPYTNYTSPNEAILCSDSYQSMYAQLLCGLLHRTDVLRVGAVFASGFI 240

Query: 241 RAIRFLEKHYTLLCHDIRTGTLNSEITDQGIRESVRKILKADGKLADFVEGECGKKCWGG 300
           RAIRFLEKHYTLLCHDIRTGTLNSEITDQGIRESVRKILKADGKLADFVEGECGKKCWGG
Sbjct: 241 RAIRFLEKHYTLLCHDIRTGTLNSEITDQGIRESVRKILKADGKLADFVEGECGKKCWGG 300

Query: 301 IISRIWPNTKYVDVIVTGTMSQYIGTLDYYSNGLPIVCTMYASSECYFGVNLKPMCKPGE 360
           IISRIWPNTKYVDVIVTGTMSQYIGTLDYYSNGLPIVCTMYASSECYFGVNLKPMCKPGE
Sbjct: 301 IISRIWPNTKYVDVIVTGTMSQYIGTLDYYSNGLPIVCTMYASSECYFGVNLKPMCKPGE 360

Query: 361 VAYTLIPTMAYFEFLPVNRSKEKELEKELVDLVDVKLGHEYELVVTTYSGLYRYRVGDIL 420
           VAYTLIPTMAYFEFLPVNRSKEKELEKELVDLVDVKLGHEYELVVTTYSGLYRYRVGDIL
Sbjct: 361 VAYTLIPTMAYFEFLPVNRSKEKELEKELVDLVDVKLGHEYELVVTTYSGLYRYRVGDIL 420

Query: 421 RVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNALTHLIPFDVTLSEYTSCVD 480
           RVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNALTHLIPFDVTLSEYTSCVD
Sbjct: 421 RVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNALTHLIPFDVTLSEYTSCVD 480

Query: 481 TSNIPGHYVLYWELTLNNEIKSTPIPPSVFEDCCFAIEESLNAVYRQGRVSDKSIGPLEI 540
           TS IPGHYVLYWELT NNEIKSTPIPPSVFEDCCFAIEESLNAVYRQGRVSDKSIGPLEI
Sbjct: 481 TSKIPGHYVLYWELTPNNEIKSTPIPPSVFEDCCFAIEESLNAVYRQGRVSDKSIGPLEI 540

Query: 541 KIVETGTFDKLMDFAISLGASINQYKTPRCVKYEPIIQLLSSRVVSNYFSPKCPKWVPKQ 600
           KIVETGTFDKLMD+AISLGASINQYKTPRCVKYEPIIQLL+SR VSNYFSPKCPKWVPKQ
Sbjct: 541 KIVETGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRTVSNYFSPKCPKWVPKQ 600

Query: 601 W 602
           W
Sbjct: 601 W 601

[Close]
BLAST of CSPI03G23650 vs. NCBI nr
Match: XP_038896877.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Benincasa hispida])

HSP 1 Score: 1144.8 bits (2960), Expect = 0.0e+00
Identity = 571/608 (93.91%), Postives = 584/608 (96.05%), Query Frame = 0 

Query: 1   MPEETPQNSSFL-STTEYYKLLEKNKKTLQFIEDVTANADQVQRRVLTEILSRNAGVEYL 60
           MPEETPQ SSFL STTEYYKLLEKNKKTLQFIEDVTANAD VQRRVLTEIL+RNA VEYL
Sbjct: 1   MPEETPQKSSFLSSTTEYYKLLEKNKKTLQFIEDVTANADHVQRRVLTEILTRNADVEYL 60

Query: 61  RRHGLGGATDCKTFKNTLPLISYEDIQPDINRIANGDSSSILCSSPISEFLTSSGTSGGE 120
           RRHGLGGATD KTFK TLPLISYEDIQPDINRIANGDSS ILCSSPISEFLTSSGTSGGE
Sbjct: 61  RRHGLGGATDRKTFKKTLPLISYEDIQPDINRIANGDSSPILCSSPISEFLTSSGTSGGE 120

Query: 121 RKLMPTIEEELERRSSLYSLLMPVMSQFIEGLEKGKGMYFLFIKSESKTPSGLLARPVLT 180
           RKLMPTIEEELERRSSLYSLLMPVMSQFIEGLEKGKGMYFLFIKSESKTPSGLLARPVLT
Sbjct: 121 RKLMPTIEEELERRSSLYSLLMPVMSQFIEGLEKGKGMYFLFIKSESKTPSGLLARPVLT 180

Query: 181 SYYKSPHFKNRPFDPYTNYTSPNEAILCSDSYQSMYAQLLCGLLHRTDVLRVGAVFASGF 240
           SYYKS HFK+RPFDPYTNYTSPN+AILCSDSYQSMYAQLLCGLLHRTDVLRVGAVFASGF
Sbjct: 181 SYYKSSHFKHRPFDPYTNYTSPNQAILCSDSYQSMYAQLLCGLLHRTDVLRVGAVFASGF 240

Query: 241 IRAIRFLEKHYTLLCHDIRTGTLNSEITDQGIRESVRKILKADGKLADFVEGECGKKCWG 300
           IRAIRFLEKH+ LLCHDIRTGTLNSEITDQGIRES+RKILK D KLADFVEGECGKKCWG
Sbjct: 241 IRAIRFLEKHFPLLCHDIRTGTLNSEITDQGIRESLRKILKPDPKLADFVEGECGKKCWG 300

Query: 301 GIISRIWPNTKYVDVIVTGTMSQYIGTLDYYSNGLPIVCTMYASSECYFGVNLKPMCKPG 360
           GIISRIWPNTKYVDVIVTGTMSQYI TLDYYSNGLPIVCTMYASSECYFGVNLKPMCKP 
Sbjct: 301 GIISRIWPNTKYVDVIVTGTMSQYIKTLDYYSNGLPIVCTMYASSECYFGVNLKPMCKPE 360

Query: 361 EVAYTLIPTMAYFEFLPVNRSK---EKELEKELVDLVDVKLGHEYELVVTTYSGLYRYRV 420
           EVAYTLIPTMAYFEFLPVNRSK   +KEL+KELVDLVDV+LG EYELVVTTY+GLYRYRV
Sbjct: 361 EVAYTLIPTMAYFEFLPVNRSKGLSDKELQKELVDLVDVQLGQEYELVVTTYAGLYRYRV 420

Query: 421 GDILRVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNALTHLIPFDVTLSEYT 480
           GD+LRVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNAL HLIPFDVTLSEYT
Sbjct: 421 GDVLRVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNALNHLIPFDVTLSEYT 480

Query: 481 SCVDTSNIPGHYVLYWELTLNNE-IKSTPIPPSVFEDCCFAIEESLNAVYRQGRVSDKSI 540
           SCVDTS IPGHYVLYWELTLNN  IK+TPIPPSVFEDCCF IEESLNAVYRQGRVSDKSI
Sbjct: 481 SCVDTSKIPGHYVLYWELTLNNNAIKTTPIPPSVFEDCCFTIEESLNAVYRQGRVSDKSI 540

Query: 541 GPLEIKIVETGTFDKLMDFAISLGASINQYKTPRCVKYEPIIQLLSSRVVSNYFSPKCPK 600
           GPLEIKIVETGTFDKLMD+AISLGASINQYKTPRCVKYEPIIQLL+SRVVSNYFSPKCPK
Sbjct: 541 GPLEIKIVETGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPKCPK 600

Query: 601 WVP--KQW 602
           WVP  KQW
Sbjct: 601 WVPGHKQW 608

[Close]
BLAST of CSPI03G23650 vs. NCBI nr
Match: KAG6592005.1 (Indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1085.1 bits (2805), Expect = 0.0e+00
Identity = 536/606 (88.45%), Postives = 565/606 (93.23%), Query Frame = 0 

Query: 1   MPEETPQNSSFLSTTEYYKLLEKNKKTLQFIEDVTANADQVQRRVLTEILSRNAGVEYLR 60
           MPEE PQ SSFLSTTEYYKLL++NKKTLQFIEDVTANA QVQRRVL EILSRNA VEYLR
Sbjct: 1   MPEEAPQKSSFLSTTEYYKLLQRNKKTLQFIEDVTANAAQVQRRVLAEILSRNAHVEYLR 60

Query: 61  RHGLGGATDCKTFKNTLPLISYEDIQPDINRIANGDSSSILCSSPISEFLTSSGTSGGER 120
           RHGL GATD KTFKNT+P+ISYEDIQ DINRIANGDSS ILCSSPISEFLTSSGTSGGER
Sbjct: 61  RHGLAGATDPKTFKNTIPVISYEDIQHDINRIANGDSSPILCSSPISEFLTSSGTSGGER 120

Query: 121 KLMPTIEEELERRSSLYSLLMPVMSQFIEGLEKGKGMYFLFIKSESKTPSGLLARPVLTS 180
           KLMPTIEEELERRS LYSLLMPVMSQF+ GLEKGKGMYFLFIKSESKTPSGLLARPVLTS
Sbjct: 121 KLMPTIEEELERRSLLYSLLMPVMSQFMGGLEKGKGMYFLFIKSESKTPSGLLARPVLTS 180

Query: 181 YYKSPHFKNRPFDPYTNYTSPNEAILCSDSYQSMYAQLLCGLLHRTDVLRVGAVFASGFI 240
           YYKS HFK+RPFDPYTNYTSPNEAILCSD+YQSMYAQLLCGL HRTDVLRVGAVFASGFI
Sbjct: 181 YYKSAHFKHRPFDPYTNYTSPNEAILCSDAYQSMYAQLLCGLHHRTDVLRVGAVFASGFI 240

Query: 241 RAIRFLEKHYTLLCHDIRTGTLNSEITDQGIRESVRKILKADGKLADFVEGECGKKCWGG 300
           RAIRFLEKH+ LLCHDIRTGTLN +ITDQ IRES++K LK D KLADF+EGEC KK W G
Sbjct: 241 RAIRFLEKHFHLLCHDIRTGTLNPQITDQPIRESLKKTLKPDPKLADFIEGECSKKSWHG 300

Query: 301 IISRIWPNTKYVDVIVTGTMSQYIGTLDYYSNGLPIVCTMYASSECYFGVNLKPMCKPGE 360
           II+RIWPNTKYVDVIVTGTMSQYI TLDYYSNGLP+VCTMYASSECYFGVNL P+CKP E
Sbjct: 301 IITRIWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPICKPAE 360

Query: 361 VAYTLIPTMAYFEFLPVNRS---KEKELEKELVDLVDVKLGHEYELVVTTYSGLYRYRVG 420
           V+YTLIPTMAYFEFLPVNR+    EKEL+ ELVDLVDV+LG EYELVVTTY+GLYRYRVG
Sbjct: 361 VSYTLIPTMAYFEFLPVNRNMSLTEKELQLELVDLVDVQLGQEYELVVTTYAGLYRYRVG 420

Query: 421 DILRVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNALTHLIPFDVTLSEYTS 480
           D+LRVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNAL+HL+PFD T+SEYTS
Sbjct: 421 DVLRVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNALSHLVPFDATVSEYTS 480

Query: 481 CVDTSNIPGHYVLYWELTLNNEIKSTPIPPSVFEDCCFAIEESLNAVYRQGRVSDKSIGP 540
           C DTS IPGHYVLYWEL++N   K+TPIPPSVFEDCC  IEESLNAVYRQGRVSDKSIGP
Sbjct: 481 CADTSKIPGHYVLYWELSVNG-AKTTPIPPSVFEDCCLTIEESLNAVYRQGRVSDKSIGP 540

Query: 541 LEIKIVETGTFDKLMDFAISLGASINQYKTPRCVKYEPIIQLLSSRVVSNYFSPKCPKWV 600
           LEIKIVETGTFDKLMD+AISLGASINQYKTPRCVKYEPIIQLL+SRVVSNYFSPKCPKWV
Sbjct: 541 LEIKIVETGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPKCPKWV 600

Query: 601 P--KQW 602
           P  KQW
Sbjct: 601 PGHKQW 605

[Close]
BLAST of CSPI03G23650 vs. NCBI nr
Match: KAG7024880.1 (Indole-3-acetic acid-amido synthetase GH3.6, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1083.9 bits (2802), Expect = 0.0e+00
Identity = 535/606 (88.28%), Postives = 565/606 (93.23%), Query Frame = 0 

Query: 1   MPEETPQNSSFLSTTEYYKLLEKNKKTLQFIEDVTANADQVQRRVLTEILSRNAGVEYLR 60
           MPEE PQ SSFLSTTEYYKLL++NKKTLQFIEDVTANA QVQRRVL EILSRNA VEYLR
Sbjct: 1   MPEEAPQKSSFLSTTEYYKLLQRNKKTLQFIEDVTANAAQVQRRVLAEILSRNAHVEYLR 60

Query: 61  RHGLGGATDCKTFKNTLPLISYEDIQPDINRIANGDSSSILCSSPISEFLTSSGTSGGER 120
           RHGL GATD KTFKNT+P+ISYEDIQ DINRIANGDSS ILCSSPISEFLTSSGTSGGER
Sbjct: 61  RHGLAGATDPKTFKNTIPVISYEDIQHDINRIANGDSSPILCSSPISEFLTSSGTSGGER 120

Query: 121 KLMPTIEEELERRSSLYSLLMPVMSQFIEGLEKGKGMYFLFIKSESKTPSGLLARPVLTS 180
           KLMPTIEEELERRS LYSLLMPVM+QF+ GLEKGKGMYFLFIKSESKTPSGLLARPVLTS
Sbjct: 121 KLMPTIEEELERRSLLYSLLMPVMNQFMGGLEKGKGMYFLFIKSESKTPSGLLARPVLTS 180

Query: 181 YYKSPHFKNRPFDPYTNYTSPNEAILCSDSYQSMYAQLLCGLLHRTDVLRVGAVFASGFI 240
           YYKS HFK+RPFDPYTNYTSPNEAILCSD+YQSMYAQLLCGL HRTDVLRVGAVFASGFI
Sbjct: 181 YYKSAHFKHRPFDPYTNYTSPNEAILCSDAYQSMYAQLLCGLHHRTDVLRVGAVFASGFI 240

Query: 241 RAIRFLEKHYTLLCHDIRTGTLNSEITDQGIRESVRKILKADGKLADFVEGECGKKCWGG 300
           RAIRFLEKH+ LLCHDIRTGTLN +ITDQ IRES++K LK D KLADF+EGEC KK W G
Sbjct: 241 RAIRFLEKHFHLLCHDIRTGTLNPQITDQPIRESLKKTLKPDPKLADFIEGECSKKSWHG 300

Query: 301 IISRIWPNTKYVDVIVTGTMSQYIGTLDYYSNGLPIVCTMYASSECYFGVNLKPMCKPGE 360
           II+RIWPNTKYVDVIVTGTMSQYI TLDYYSNGLP+VCTMYASSECYFGVNL P+CKP E
Sbjct: 301 IITRIWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPICKPAE 360

Query: 361 VAYTLIPTMAYFEFLPVNRS---KEKELEKELVDLVDVKLGHEYELVVTTYSGLYRYRVG 420
           V+YTLIPTMAYFEFLPVNR+    EKEL+ ELVDLVDV+LG EYELVVTTY+GLYRYRVG
Sbjct: 361 VSYTLIPTMAYFEFLPVNRNMSLTEKELQLELVDLVDVQLGQEYELVVTTYAGLYRYRVG 420

Query: 421 DILRVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNALTHLIPFDVTLSEYTS 480
           D+LRVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNAL+HL+PFD T+SEYTS
Sbjct: 421 DVLRVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNALSHLVPFDATVSEYTS 480

Query: 481 CVDTSNIPGHYVLYWELTLNNEIKSTPIPPSVFEDCCFAIEESLNAVYRQGRVSDKSIGP 540
           C DTS IPGHYVLYWEL++N   K+TPIPPSVFEDCC  IEESLNAVYRQGRVSDKSIGP
Sbjct: 481 CADTSKIPGHYVLYWELSVNG-AKTTPIPPSVFEDCCLTIEESLNAVYRQGRVSDKSIGP 540

Query: 541 LEIKIVETGTFDKLMDFAISLGASINQYKTPRCVKYEPIIQLLSSRVVSNYFSPKCPKWV 600
           LEIKIVETGTFDKLMD+AISLGASINQYKTPRCVKYEPIIQLL+SRVVSNYFSPKCPKWV
Sbjct: 541 LEIKIVETGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPKCPKWV 600

Query: 601 P--KQW 602
           P  KQW
Sbjct: 601 PGHKQW 605

[Close]
BLAST of CSPI03G23650 vs. TAIR 10
Match: AT5G54510.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 963.0 bits (2488), Expect = 1.1e-280
Identity = 472/597 (79.06%), Postives = 523/597 (87.60%), Query Frame = 0 

Query: 20  LLEKNKKTLQFIEDVTANADQVQRRVLTEILSRNAGVEYLRRHGLGGATDCKTFKNTLPL 79
           L EKNK  LQFIEDVT NAD VQRRVL EILSRNA VEYL+RHGL G TD +TFK+ +P+
Sbjct: 17  LAEKNKNKLQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHGLEGRTDRETFKHIMPV 76

Query: 80  ISYEDIQPDINRIANGDSSSILCSSPISEFLTSSGTSGGERKLMPTIEEELERRSSLYSL 139
           ++YEDIQP+INRIANGD S +LCS+PISEFLTSSGTSGGERKLMPTIEEEL+RRS LYSL
Sbjct: 77  VTYEDIQPEINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSL 136

Query: 140 LMPVMSQFIEGLEKGKGMYFLFIKSESKTPSGLLARPVLTSYYKSPHFKNRPFDPYTNYT 199
           LMPVM QF+ GL+KGKGMYFLFIKSESKTP GL ARPVLTSYYKS HFKNRP+DPYTNYT
Sbjct: 137 LMPVMDQFVPGLDKGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKNRPYDPYTNYT 196

Query: 200 SPNEAILCSDSYQSMYAQLLCGLLHRTDVLRVGAVFASGFIRAIRFLEKHYTLLCHDIRT 259
           SPN+ ILCSDSYQSMY+Q+LCGL    +VLRVGAVFASGFIRAI+FLEKH+  L  DIRT
Sbjct: 197 SPNQTILCSDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAIKFLEKHWPELARDIRT 256

Query: 260 GTLNSEITDQGIRESVRKILKADGKLADFVEGECGKKCWGGIISRIWPNTKYVDVIVTGT 319
           GTL+SEITD  +RE+V +ILK D KLADFVE EC K  W GII+R+WPNTKYVDVIVTGT
Sbjct: 257 GTLSSEITDSSVREAVGEILKPDPKLADFVESECRKTSWQGIITRLWPNTKYVDVIVTGT 316

Query: 320 MSQYIGTLDYYSNGLPIVCTMYASSECYFGVNLKPMCKPGEVAYTLIPTMAYFEFLPVNR 379
           MSQYI TLDYYSNGLP+VCTMYASSECYFGVNL+P+CKP EV+YTLIP MAYFEFLPV+R
Sbjct: 317 MSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPNMAYFEFLPVHR 376

Query: 380 SK-------------EKELEKELVDLVDVKLGHEYELVVTTYSGLYRYRVGDILRVAGFK 439
           +              EKE ++ELVDLVDVKLG EYELVVTTY+GLYRYRVGD+L VAGFK
Sbjct: 377 NSGVTSSISLPKALTEKE-QQELVDLVDVKLGQEYELVVTTYAGLYRYRVGDVLSVAGFK 436

Query: 440 NNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNALTHLIPFDVTLSEYTSCVDTSNIPG 499
           NNAPQF+FICRKNV LSIDSDKTDEVELQNAVKNA+THL+PFD +LSEYTS  DTS+IPG
Sbjct: 437 NNAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIPG 496

Query: 500 HYVLYWELTLNNEIKSTPIPPSVFEDCCFAIEESLNAVYRQGRVSDKSIGPLEIKIVETG 559
           HYVL+WEL LN    +TPIPPSVFEDCC  IEESLN+VYRQGRVSDKSIGPLEIK+VE+G
Sbjct: 497 HYVLFWELCLNG---NTPIPPSVFEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKMVESG 556

Query: 560 TFDKLMDFAISLGASINQYKTPRCVKYEPIIQLLSSRVVSNYFSPKCPKWVP--KQW 602
           TFDKLMD+AISLGASINQYKTPRCVK+ PII+LL+SRVV +YFSPKCPKW P  KQW
Sbjct: 557 TFDKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWSPGHKQW 609

[Close]
BLAST of CSPI03G23650 vs. TAIR 10
Match: AT4G27260.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 941.0 bits (2431), Expect = 4.6e-274
Identity = 465/595 (78.15%), Postives = 516/595 (86.72%), Query Frame = 0 

Query: 22  EKNKKTLQFIEDVTANADQVQRRVLTEILSRNAGVEYLRRHGLGGATDCKTFKNTLPLIS 81
           +KNK+ LQ IE++T+NADQVQR+VL EIL+RNA VEYLRRH L G TD +TFKN +P+I+
Sbjct: 19  QKNKQKLQLIEELTSNADQVQRQVLEEILTRNADVEYLRRHDLNGRTDRETFKNIMPVIT 78

Query: 82  YEDIQPDINRIANGDSSSILCSSPISEFLTSSGTSGGERKLMPTIEEELERRSSLYSLLM 141
           YEDI+P+INRIANGD S IL S PISEFLTSSGTSGGERKLMPTIEEEL+RRS LYSLLM
Sbjct: 79  YEDIEPEINRIANGDKSPILSSKPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSLLM 138

Query: 142 PVMSQFIEGLEKGKGMYFLFIKSESKTPSGLLARPVLTSYYKSPHFKNRPFDPYTNYTSP 201
           PVMSQF+ GLE GKGMYFLFIKSESKTP GL ARPVLTSYYKS HFK RP+DPYTNYTSP
Sbjct: 139 PVMSQFVPGLENGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKERPYDPYTNYTSP 198

Query: 202 NEAILCSDSYQSMYAQLLCGLLHRTDVLRVGAVFASGFIRAIRFLEKHYTLLCHDIRTGT 261
           NE ILCSDSYQSMY+Q+LCGL    +VLRVGAVFASGFIRAI+FLEKH+  L  DIRTGT
Sbjct: 199 NETILCSDSYQSMYSQMLCGLCQHQEVLRVGAVFASGFIRAIKFLEKHWIELVRDIRTGT 258

Query: 262 LNSEITDQGIRESVRKILKADGKLADFVEGECGKKCWGGIISRIWPNTKYVDVIVTGTMS 321
           L+S ITD  +RE+V KILK   KLADFVE EC K  W GII+R+WPNTKYVDVIVTGTMS
Sbjct: 259 LSSLITDPSVREAVAKILKPSPKLADFVEFECKKSSWQGIITRLWPNTKYVDVIVTGTMS 318

Query: 322 QYIGTLDYYSNGLPIVCTMYASSECYFGVNLKPMCKPGEVAYTLIPTMAYFEFLPVNRSK 381
           QYI TLDYYSNGLP+VCTMYASSECYFGVNL+P+CKP EV+YTLIP+MAYFEFLPV+R+ 
Sbjct: 319 QYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPSMAYFEFLPVHRNN 378

Query: 382 -------------EKELEKELVDLVDVKLGHEYELVVTTYSGLYRYRVGDILRVAGFKNN 441
                        EKE ++ELVDLVDVKLG EYELVVTTY+GL RYRVGD+LRV GFKN 
Sbjct: 379 GVTNSINLPKALTEKE-QQELVDLVDVKLGQEYELVVTTYAGLCRYRVGDLLRVTGFKNK 438

Query: 442 APQFNFICRKNVALSIDSDKTDEVELQNAVKNALTHLIPFDVTLSEYTSCVDTSNIPGHY 501
           APQF+FICRKNV LSIDSDKTDEVELQNAVKNA+THL+PFD +LSEYTS  DTS+IPGHY
Sbjct: 439 APQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIPGHY 498

Query: 502 VLYWELTLNNEIKSTPIPPSVFEDCCFAIEESLNAVYRQGRVSDKSIGPLEIKIVETGTF 561
           VL+WEL L+    +TPIPPSVFEDCC A+EES N VYRQGRVSDKSIGPLEIKIVE GTF
Sbjct: 499 VLFWELCLDG---NTPIPPSVFEDCCLAVEESFNTVYRQGRVSDKSIGPLEIKIVEPGTF 558

Query: 562 DKLMDFAISLGASINQYKTPRCVKYEPIIQLLSSRVVSNYFSPKCPKWVP--KQW 602
           DKLMD+AISLGASINQYKTPRCVK+ PII+LL+SRVV +YFSPKCPKWVP  KQW
Sbjct: 559 DKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWVPGHKQW 609

[Close]
BLAST of CSPI03G23650 vs. TAIR 10
Match: AT2G14960.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 760.4 bits (1962), Expect = 1.1e-219
Identity = 375/580 (64.66%), Postives = 450/580 (77.59%), Query Frame = 0 

Query: 22  EKNKKTLQFIEDVTANADQVQRRVLTEILSRNAGVEYLRRHGLGGATDCKTFKNTLPLIS 81
           EK+ K L+FIE++T NAD VQ  +L EIL+RNA  EYLRR  L GATD  TFK  +P+I+
Sbjct: 17  EKDAKALRFIEEMTRNADTVQENLLAEILARNADTEYLRRFNLCGATDRDTFKTKIPVIT 76

Query: 82  YEDIQPDINRIANGDSSSILCSSPISEFLTSSGTSGGERKLMPTIEEELERRSSLYSLLM 141
           YED+QP+I RIA+GD S IL + PISEFLTSSGTS GERKLMPTI+EEL+RR  LYSLLM
Sbjct: 77  YEDLQPEIQRIADGDRSPILSAHPISEFLTSSGTSAGERKLMPTIKEELDRRQLLYSLLM 136

Query: 142 PVMSQFIEGLEKGKGMYFLFIKSESKTPSGLLARPVLTSYYKSPHFKNRPFDPYTNYTSP 201
           PVM+ ++ GL+KGKGMYFLF+KSE+KTP GL ARPVLTSYYKS HF++RP+DPY  YTSP
Sbjct: 137 PVMNLYVPGLDKGKGMYFLFVKSETKTPGGLPARPVLTSYYKSEHFRSRPYDPYNVYTSP 196

Query: 202 NEAILCSDSYQSMYAQLLCGLLHRTDVLRVGAVFASGFIRAIRFLEKHYTLLCHDIRTGT 261
           NEAILC DS+QSMY Q+LCGLL R  VLRVGAVFASG +RAIRFL+ H++   HDI  G 
Sbjct: 197 NEAILCPDSFQSMYTQMLCGLLDRLSVLRVGAVFASGLLRAIRFLQLHWSRFAHDIELGC 256

Query: 262 LNSEITDQGIRESVRKILKADGKLADFVEGECGKKCWGGIISRIWPNTKYVDVIVTGTMS 321
           L+SEITD  IR+ +  ILK D  LA+F+  EC    W  II+RIWPNTKY+DVIVTG M+
Sbjct: 257 LDSEITDPSIRQCMSGILKPDPVLAEFIRRECKSDNWEKIITRIWPNTKYLDVIVTGAMA 316

Query: 322 QYIGTLDYYSNGLPIVCTMYASSECYFGVNLKPMCKPGEVAYTLIPTMAYFEFLPVNRSK 381
           QYI TL+YYS GLP+ CTMYASSECYFG+NL PM KP EV+YT++P MAYFEF+P+  +K
Sbjct: 317 QYIPTLEYYSGGLPMACTMYASSECYFGLNLNPMSKPSEVSYTIMPNMAYFEFIPLGGTK 376

Query: 382 EKELEKELVDLVDVKLGHEYELVVTTYSGLYRYRVGDILRVAGFKNNAPQFNFICRKNVA 441
                   V+LVDV +G EYELVVTTY+GL RYRVGDILRV GF N+APQF+F+ RKNV 
Sbjct: 377 -------AVELVDVNIGKEYELVVTTYAGLCRYRVGDILRVTGFHNSAPQFHFVRRKNVL 436

Query: 442 LSIDSDKTDEVELQNAVKNALTHL-IPFDVTLSEYTSCVDTSNIPGHYVLYWELTLNNEI 501
           LSIDSDKTDE ELQ AV+NA + L       ++EYTS  DTS IPGHYVLYWEL + +  
Sbjct: 437 LSIDSDKTDESELQKAVENASSILHEECGSRVAEYTSYADTSTIPGHYVLYWELLVRDGA 496

Query: 502 KSTPIPPSVFEDCCFAIEESLNAVYRQGRVSDKSIGPLEIKIVETGTFDKLMDFAISLGA 561
           +           CC  +EESLN+VYRQ RV+D S+GPLEI++V  GTF++LMD+AIS GA
Sbjct: 497 RQP--SHETLTRCCLGMEESLNSVYRQSRVADNSVGPLEIRVVRNGTFEELMDYAISRGA 556

Query: 562 SINQYKTPRCVKYEPIIQLLSSRVVSNYFSPKCPKWVPKQ 601
           SINQYK PRCV + PI++LL SRVVS +FSP  P W P++
Sbjct: 557 SINQYKVPRCVNFTPIVELLDSRVVSAHFSPSLPHWTPER 587

[Close]
BLAST of CSPI03G23650 vs. TAIR 10
Match: AT4G37390.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 753.8 bits (1945), Expect = 1.0e-217
Identity = 377/606 (62.21%), Postives = 463/606 (76.40%), Query Frame = 0 

Query: 1   MPEETPQNSSFLSTTEYYKLLEKNKKTLQFIEDVTANADQVQRRVLTEILSRNAGVEYLR 60
           M  ++P  S  +S T      EK+ K L+FIE++T N D VQ +VL EIL+RN+  EYL+
Sbjct: 1   MAVDSPLQSRMVSAT----TSEKDVKALKFIEEMTRNPDSVQEKVLGEILTRNSNTEYLK 60

Query: 61  RHGLGGATDCKTFKNTLPLISYEDIQPDINRIANGDSSSILCSSPISEFLTSSGTSGGER 120
           R  L G  D KTFK+ +P+++YED++P+I RI+NGD S IL S PI+EFLTSSGTS GER
Sbjct: 61  RFDLDGVVDRKTFKSKVPVVTYEDLKPEIQRISNGDCSPILSSHPITEFLTSSGTSAGER 120

Query: 121 KLMPTIEEELERRSSLYSLLMPVMSQFIEGLEKGKGMYFLFIKSESKTPSGLLARPVLTS 180
           KLMPTIEE+L+RR  LYSLLMPVM+ ++ GL+KGKG+YFLF+KSESKT  GL ARPVLTS
Sbjct: 121 KLMPTIEEDLDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSESKTSGGLPARPVLTS 180

Query: 181 YYKSPHFKNRPFDPYTNYTSPNEAILCSDSYQSMYAQLLCGLLHRTDVLRVGAVFASGFI 240
           YYKS HFK RP+DPY  YTSPNEAILCSDS QSMYAQ+LCGLL R +VLR+GAVFASG +
Sbjct: 181 YYKSDHFKRRPYDPYNVYTSPNEAILCSDSSQSMYAQMLCGLLMRHEVLRLGAVFASGLL 240

Query: 241 RAIRFLEKHYTLLCHDIRTGTLNSEITDQGIRESVRKIL-KADGKLADFVEGECGKKCWG 300
           RAI FL+ ++  L  DI TGTL+S I D  I+  + KIL K D +LA+F+ G C ++ W 
Sbjct: 241 RAISFLQNNWKELARDISTGTLSSRIFDPAIKNRMSKILTKPDQELAEFLVGVCSQENWE 300

Query: 301 GIISRIWPNTKYVDVIVTGTMSQYIGTLDYYSNGLPIVCTMYASSECYFGVNLKPMCKPG 360
           GII++IWPNTKY+DVIVTG M+QYI TL+YYS GLP+ CTMYASSE YFG+NLKPMCKP 
Sbjct: 301 GIITKIWPNTKYLDVIVTGAMAQYIPTLEYYSGGLPMACTMYASSESYFGINLKPMCKPS 360

Query: 361 EVAYTLIPTMAYFEFLPVNR----SKEKEL-EKELVDLVDVKLGHEYELVVTTYSGLYRY 420
           EV+YT++P MAYFEFLP N     + E  L E  LV+L +V++G EYELV+TTY+GLYRY
Sbjct: 361 EVSYTIMPNMAYFEFLPHNHDGDGAAEASLDETSLVELANVEVGKEYELVITTYAGLYRY 420

Query: 421 RVGDILRVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNALTHLIPFDVTLSE 480
           RVGDILRV GF N+APQF FI RKNV LS++SDKTDE ELQ AV+NA          + E
Sbjct: 421 RVGDILRVTGFHNSAPQFKFIRRKNVLLSVESDKTDEAELQKAVENASRLFAEQGTRVIE 480

Query: 481 YTSCVDTSNIPGHYVLYWELTLNNEIKSTPIPPSVFEDCCFAIEESLNAVYRQGRVSDKS 540
           YTS  +T  IPGHYV+YWEL L  +  +  +   V   CC  +EESLN+VYRQ RV+DKS
Sbjct: 481 YTSYAETKTIPGHYVIYWEL-LGRDQSNALMSEEVMAKCCLEMEESLNSVYRQSRVADKS 540

Query: 541 IGPLEIKIVETGTFDKLMDFAISLGASINQYKTPRCVKYEPIIQLLSSRVVSNYFSPKCP 600
           IGPLEI++V  GTF++LMD+AIS GASINQYK PRCV + PI++LL SRVVS +FSP  P
Sbjct: 541 IGPLEIRVVRNGTFEELMDYAISRGASINQYKVPRCVSFTPIMELLDSRVVSAHFSPSLP 600

[Close]
BLAST of CSPI03G23650 vs. TAIR 10
Match: AT2G23170.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 741.5 bits (1913), Expect = 5.4e-214
Identity = 366/580 (63.10%), Postives = 454/580 (78.28%), Query Frame = 0 

Query: 23  KNKKTLQFIEDVTANADQVQRRVLTEILSRNAGVEYLRRHGLGGATDCKTFKNTLPLISY 82
           K+ K L+FIE++T N D VQ++V+ EILSRN+  EYL+R GL G TD KTFK  +P++ Y
Sbjct: 18  KDVKALRFIEEMTRNVDFVQKKVIREILSRNSDTEYLKRFGLKGFTDRKTFKTKVPVVIY 77

Query: 83  EDIQPDINRIANGDSSSILCSSPISEFLTSSGTSGGERKLMPTIEEELERRSSLYSLLMP 142
           +D++P+I RIANGD S IL S PI+EFLTSSGTS GERKLMPTI+E+++RR  LYSLLMP
Sbjct: 78  DDLKPEIQRIANGDRSMILSSYPITEFLTSSGTSAGERKLMPTIDEDMDRRQLLYSLLMP 137

Query: 143 VMSQFIEGLEKGKGMYFLFIKSESKTPSGLLARPVLTSYYKSPHFKNRPFDPYTNYTSPN 202
           VM+ ++ GL+KGK +YFLF+K+ESKTP GL ARPVLTSYYKS  FK RP DPY  YTSPN
Sbjct: 138 VMNLYVPGLDKGKALYFLFVKTESKTPGGLPARPVLTSYYKSEQFKRRPNDPYNVYTSPN 197

Query: 203 EAILCSDSYQSMYAQLLCGLLHRTDVLRVGAVFASGFIRAIRFLEKHYTLLCHDIRTGTL 262
           EAILC DS QSMY Q+LCGLL R +VLR+GAVFASG +RAI FL+ ++  L  DI TGTL
Sbjct: 198 EAILCPDSSQSMYTQMLCGLLMRHEVLRLGAVFASGLLRAIGFLQTNWKELADDISTGTL 257

Query: 263 NSEITDQGIRESVRKIL-KADGKLADFVEGECGK-KCWGGIISRIWPNTKYVDVIVTGTM 322
           +S I+D  I+ES+ KIL K D +LADF+   CG+   W GII++IWPNTKY+DVIVTG M
Sbjct: 258 SSRISDPAIKESMSKILTKPDQELADFITSVCGQDNSWEGIITKIWPNTKYLDVIVTGAM 317

Query: 323 SQYIGTLDYYSNGLPIVCTMYASSECYFGVNLKPMCKPGEVAYTLIPTMAYFEFLPVNRS 382
           +QYI  L+YYS GLP+ CTMYASSE YFG+NLKPMCKP EV+YT++P MAYFEFLP +  
Sbjct: 318 AQYIPMLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSYTIMPNMAYFEFLPHHEV 377

Query: 383 KEKELEKELVDLVDVKLGHEYELVVTTYSGLYRYRVGDILRVAGFKNNAPQFNFICRKNV 442
             +  + ELV+L DV++G EYELV+TTY+GL RYRVGDIL+V GF N+APQF F+ RKNV
Sbjct: 378 PTE--KSELVELADVEVGKEYELVITTYAGLNRYRVGDILQVTGFYNSAPQFKFVRRKNV 437

Query: 443 ALSIDSDKTDEVELQNAVKNALTHLIPFDVTLSEYTSCVDTSNIPGHYVLYWELTLNNEI 502
            LSI+SDKTDE ELQ+AV+NA   L      + EYTS  +T  IPGHYV+YWEL + ++ 
Sbjct: 438 LLSIESDKTDEAELQSAVENASLLLGEQGTRVIEYTSYAETKTIPGHYVIYWELLVKDQ- 497

Query: 503 KSTPIPPSVFEDCCFAIEESLNAVYRQGRVSDKSIGPLEIKIVETGTFDKLMDFAISLGA 562
            + P    V   CC  +EESLN+VYRQ RV+DKSIGPLEI++V+ GTF++LMD+AIS GA
Sbjct: 498 -TNPPNDEVMARCCLEMEESLNSVYRQSRVADKSIGPLEIRVVKNGTFEELMDYAISRGA 557

Query: 563 SINQYKTPRCVKYEPIIQLLSSRVVSNYFSPKCPKWVPKQ 601
           SINQYK PRCV + PI++LL SRVVS +FSP  P W P++
Sbjct: 558 SINQYKVPRCVSFTPIMELLDSRVVSTHFSPALPHWSPER 593

The following BLAST results are available for this feature:
BLAST of CSPI03G23650 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9LSQ41.6e-27979.06Indole-3-acetic acid-amido synthetase GH3.6 OS=Arabidopsis thaliana OX=3702 GN=G... [more]
O818296.5e-27378.15Indole-3-acetic acid-amido synthetase GH3.5 OS=Arabidopsis thaliana OX=3702 GN=G... [more]
Q8LQM54.0e-23868.50Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Oryza sativa subsp. japo... [more]
Q60EJ62.9e-22866.22Probable indole-3-acetic acid-amido synthetase GH3.4 OS=Oryza sativa subsp. japo... [more]
O823331.6e-21864.66Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Arabidopsis thaliana OX=... [more]
BLAST of CSPI03G23650 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LAR60.0e+00100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G431430 PE=3 SV=1[more]
A0A5A7V8A50.0e+0098.50Indole-3-acetic acid-amido synthetase GH3.6 OS=Cucumis melo var. makuwa OX=11946... [more]
A0A1S3BYK90.0e+0098.50indole-3-acetic acid-amido synthetase GH3.6 OS=Cucumis melo OX=3656 GN=LOC103494... [more]
A0A6J1F6T20.0e+0088.28indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita moschata OX=3662 G... [more]
A0A6J1IM750.0e+0087.79indole-3-acetic acid-amido synthetase GH3.6 OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
BLAST of CSPI03G23650 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_004149186.10.0e+00100.00indole-3-acetic acid-amido synthetase GH3.6 [Cucumis sativus] >KGN58009.1 hypoth... [more]
XP_008454612.10.0e+0098.50PREDICTED: indole-3-acetic acid-amido synthetase GH3.6 [Cucumis melo] >KAA006343... [more]
XP_038896877.10.0e+0093.91indole-3-acetic acid-amido synthetase GH3.6-like [Benincasa hispida][more]
KAG6592005.10.0e+0088.45Indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma subsp. so... [more]
KAG7024880.10.0e+0088.28Indole-3-acetic acid-amido synthetase GH3.6, partial [Cucurbita argyrosperma sub... [more]
BLAST of CSPI03G23650 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G54510.11.1e-28079.06Auxin-responsive GH3 family protein [more]
AT4G27260.14.6e-27478.15Auxin-responsive GH3 family protein [more]
AT2G14960.11.1e-21964.66Auxin-responsive GH3 family protein [more]
AT4G37390.11.0e-21762.21Auxin-responsive GH3 family protein [more]
AT2G23170.15.4e-21463.10Auxin-responsive GH3 family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004993GH3 familyPFAMPF03321GH3coord: 31..573
e-value: 4.1E-200
score: 666.1
IPR004993GH3 familyPANTHERPTHR31901GH3 DOMAIN-CONTAINING PROTEINcoord: 14..599
NoneNo IPR availablePANTHERPTHR31901:SF70BNAC03G14570D PROTEINcoord: 14..599

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI03G23650.1CSPI03G23650.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005737 cytoplasm
molecular_function GO:0010279 indole-3-acetic acid amido synthetase activity