CSPI03G23430 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI03G23430
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionDDRGK domain-containing protein 1-like
LocationChr3: 20305254 .. 20308714 (-)
RNA-Seq ExpressionCSPI03G23430
SyntenyCSPI03G23430
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CGACATCGTATTCTTGCCGCTCCTCTCGTTACGGTTTCTTTCGCTTTTGTTTGTTCATTCTCATCGGCGATCTTCAGTTCCCAAGCACCACTTTCTGATTCCTTCTCGCAAGGTACGCTCTTTAATGCCTTCTATATCGTAATTGTTCGTAAATATTCTGTTTAAGAAGTTAGATCTTTCTTAGAGATGTTTCGTTTGCGAATTTTCAAGCTATTAAACAAGTTGAAACGATGGAATTTTTGGTCATAGGTGTAGAGAAGTTGCAAAATGGAGGAATTTCTTGTGGCAGTTCTCTCGATGCTTCTTGTTGCTGCATTGGTTCCTCTGTTTCTGTGGCGACGCTATCAGGACTCCCGCTCCGGTCATGCACACGAAGATGAAGATCAGGTGTGGTTTTGGAGGATGGCTGATGAGTTCTTTGAGGGTTTATTTATTTGTTTTGTATTGAAATGGTATTGTCTTGGTTATCAATCTGTTTCGAAGAAAGGGTGTCGGGGGGTTTTTACTTGGTTTTTGGAATCTTTCAGGTTCCTCAGAGAGAAACGGTGGTTCGTGCTACTGGTGGTGGCCGTCGTATGCGCCGGAGACCTGCTATGGCTGCTAGTTCGTCGAATGCAGAGGCAAGTCTGGAAGGTTTGTATATCAATTAGCTTTCTAAGTCTTTTTTTTTTTTTATTTAATTTCATTAGTTTGTTATGTTGTTAATATCAATTGTAAGGGAGTATGGGGGCCATGTAGCTCAAGCTCGATTCACTTGGAAACTCTCCAATCAGTTGATTGATGTGTTTTAAATTAAAATGAAGTGTACACGACCGCATGCCACATCTGAATTGGTTAAGTAGTTGTAAATAGTAATCACTTAATTTCCTCCCTTCCAAATTTCTTCAAGTTTAGATTCAAATGTCGATATGCACTGTTAAAAAAAACATTGAATTATGACCAAAGACTATTAAAAACTGGGACGGGAAGTAGCTAAAGGGAGATCACCTACCTACTGCCTTCCGTTCCATATCCATTTCATCCATATGGTATATTTAACATAACGTGTTGCATCTGCTTGGATTTATATCTATATACACATAGATAGATTATGTTTATCTAGACATCAAGTGTACAGATTTTGTACGAGCTTGTCAATCTTTACCTCATAACAAAGAAGGTGAATGAGAAATTGATGGCTGTTGACCGCATGTTATTGGTATATTGAGCAAAAAGAACTTAAATATAGAGTTTTTTATTGTAATAATTAGCCAATACGGAGTTCGAAAATGCTTGGAATAACCAATGTGTGATTTTAACCAAGAATTTATTATTTTGAATATGGCAACTCTTTAACAATTACTCCTGATTATACAGATAATGCTGATGGAAGTGATGATGAAATTGTGGCTGAAGAATACCATGGAGGAAGAGTGTTGAAGAAGAAAGAAAAGAAGCGACAAGAGCGGGAGGCACAAAGACAGGTAACATCGCAGTGTGGCTCTTAGGTGCAGAAAAATGCAGATGTCTTAGGTTCTTAGATTCAATGTATGGCTCTTATAGTCTTCTTCTTTTTTTCCCACAATTTTTTCTTACACTTTGTCATCTTTTCTACTCATGTTTTTGAAGGCTGAACAAGCTTCACGTGACTCAAGGTTGACAAAACAAGATCGTTATGCAGAAATACGGAGGAAAAAAGATGAGGAGCGTGAAGAGCAGGAACGGTTACTGGTTAGTTAATCAATCATCTTTTGTGTTGAGAATTCTAAGTGCAAAGTAGGCTGGAACTTCTGAGGTTGAGTGGTCTATTTTCTTTGTTTAGGAAGAAGAAGCCAAGGCACGGAAGGCCAAGGAGGAAGAGGCTGCTGCACTTGAGTTTGAGAAGTGGAAAGGGGCATTTTCAGTTGACGCTGAAGGTACAACTGAAGCTGAAGTGGAAGGCGGAAATCAAGATTTGGTGACTGCTTTTGTGGAATATATAAAGGTTAAGCCTGTCATGTTGTTATGAGATGCTGTCTTTTTACCTTATGAGAATTCTACCGTTGGGGACATATTTTTGTGTAGGTTGGGACTGGGAGTGAGACCTGCCCATGAATGACTAAACCTGTCTATGCTTAGTTACTGCCTGTTTAATTTATGTTTTCCCTCTGGCACTTTTTCTGAAACCCTTCGTACATTGTTTTACAGAGTCATAAATGTGTCCCATTGGAAGATCTTGCTGCAGAATTTAAATTAAGAACTCAGGTAACTCAGATTATGGTTGTTGTGGGTATGCTTTCAAGATTTAGTTGTGTTCGTAGTTCTTTTTTCTACCAAAAAAAGAAATGGAGTCAGGGAGTTTCAAGGAGAATGATTTCCCATTCTTGGCTTTTTGATTTTGTGTATGTTCTTCCCTCAGTGGTTTTATGCAAATAGTACCTCTATTTATTTCACAGTATATTTCACTAATGCAGGAATGTATTAATCGGATCACCTCCCTGGAGAGTATGGGTATGCCTCTTTCATTTTTCTTCTCTCTGTCCCTCTTGTCTAACCAATGATCATGGATTTGTTTTTCATAATTTCTTGCTAGTTTTTACATTTGAACTTTTGATTAGATATCACGAGTAACGTTAATTTCCATTCCGATTGAACAAATGCATGAATTTTATAGGACGACTTTCTGGCGTTATGGATGATAGAGGAAAATACATATACATATCAAAGGAAGAAATGCAAGCGGTAGCTGATTTCATCAAGCGTCAGGGAAGAGTTAGCATCTCACATCTAGCTAGTAAGTCCAACCAGTTCATTGATTTGGAACCAAAACTCCAGTTGGTTGAGGAAATCAATGTTGAGGAGATAGCTGTCTCATGATACACGTTAATTTTGAAAAGGAATGTTACACATCAAATAATCATTATCAACTAGTATATATTTTACCCAAAGATCGTGGGTGGAAGTCGATAAATCTTCATCTCTGCACAGGTTCTTCAGAACTATTGTTTTCATGCCTGCATTTTATGCTTGGATTCATCTCGTAATCTCATGCCTAGAAATAGAAGATGATCCATCTGTCAGGTCTTTTTGGTTTTGGAAAAATGGTTGTGATGTATGGGAAACTATTGGAGGATGTTAATAGACAGGAAGTTTTCTGAAGTGAAGCAAATGGTGGTAGCGGGCACTCGAGTGGCTAAACAATTTCGGCTGAGAATAGGACTAATGATTCACACATTTTCTTCTTGGTGTACTGTTTTTAATCGACAGAGTTAGATCCGTGAAAACAAATAATTAGTTGTTTGTCTTTGAGTAAGTGTTTTGAATGTTTATCATGTTTAGAATTGCTTAATACGAATGATTGTGATTTGAGGAAATAGTTGAGGTGGCCGGTGGGTCGTAAATTTACTCAAACCCTCACCGATTGCTTTCAATTGGTTCATGTAGTTGTCTTTAAAAATCCTAAAATTAAATGACTTTGGTGGAGTCCATGGTACGGCA

mRNA sequence

CGACATCGTATTCTTGCCGCTCCTCTCGTTACGGTTTCTTTCGCTTTTGTTTGTTCATTCTCATCGGCGATCTTCAGTTCCCAAGCACCACTTTCTGATTCCTTCTCGCAAGGTGTAGAGAAGTTGCAAAATGGAGGAATTTCTTGTGGCAGTTCTCTCGATGCTTCTTGTTGCTGCATTGGTTCCTCTGTTTCTGTGGCGACGCTATCAGGACTCCCGCTCCGGTCATGCACACGAAGATGAAGATCAGGTTCCTCAGAGAGAAACGGTGGTTCGTGCTACTGGTGGTGGCCGTCGTATGCGCCGGAGACCTGCTATGGCTGCTAGTTCGTCGAATGCAGAGGCAAGTCTGGAAGATAATGCTGATGGAAGTGATGATGAAATTGTGGCTGAAGAATACCATGGAGGAAGAGTGTTGAAGAAGAAAGAAAAGAAGCGACAAGAGCGGGAGGCACAAAGACAGGCTGAACAAGCTTCACGTGACTCAAGGTTGACAAAACAAGATCGTTATGCAGAAATACGGAGGAAAAAAGATGAGGAGCGTGAAGAGCAGGAACGGTTACTGGAAGAAGAAGCCAAGGCACGGAAGGCCAAGGAGGAAGAGGCTGCTGCACTTGAGTTTGAGAAGTGGAAAGGGGCATTTTCAGTTGACGCTGAAGGTACAACTGAAGCTGAAGTGGAAGGCGGAAATCAAGATTTGGTGACTGCTTTTGTGGAATATATAAAGAGTCATAAATGTGTCCCATTGGAAGATCTTGCTGCAGAATTTAAATTAAGAACTCAGGAATGTATTAATCGGATCACCTCCCTGGAGAGTATGGGACGACTTTCTGGCGTTATGGATGATAGAGGAAAATACATATACATATCAAAGGAAGAAATGCAAGCGGTAGCTGATTTCATCAAGCGTCAGGGAAGAGTTAGCATCTCACATCTAGCTAGTAAGTCCAACCAGTTCATTGATTTGGAACCAAAACTCCAGTTGGTTGAGGAAATCAATGTTGAGGAGATAGCTGTCTCATGATACACGTTAATTTTGAAAAGGAATGTTACACATCAAATAATCATTATCAACTAGTATATATTTTACCCAAAGATCGTGGGTGGAAGTCGATAAATCTTCATCTCTGCACAGGTTCTTCAGAACTATTGTTTTCATGCCTGCATTTTATGCTTGGATTCATCTCGTAATCTCATGCCTAGAAATAGAAGATGATCCATCTGTCAGGTCTTTTTGGTTTTGGAAAAATGGTTGTGATGTATGGGAAACTATTGGAGGATGTTAATAGACAGGAAGTTTTCTGAAGTGAAGCAAATGGTGGTAGCGGGCACTCGAGTGGCTAAACAATTTCGGCTGAGAATAGGACTAATGATTCACACATTTTCTTCTTGGTGTACTGTTTTTAATCGACAGAGTTAGATCCGTGAAAACAAATAATTAGTTGTTTGTCTTTGAGTAAGTGTTTTGAATGTTTATCATGTTTAGAATTGCTTAATACGAATGATTGTGATTTGAGGAAATAGTTGAGGTGGCCGGTGGGTCGTAAATTTACTCAAACCCTCACCGATTGCTTTCAATTGGTTCATGTAGTTGTCTTTAAAAATCCTAAAATTAAATGACTTTGGTGGAGTCCATGGTACGGCA

Coding sequence (CDS)

ATGGAGGAATTTCTTGTGGCAGTTCTCTCGATGCTTCTTGTTGCTGCATTGGTTCCTCTGTTTCTGTGGCGACGCTATCAGGACTCCCGCTCCGGTCATGCACACGAAGATGAAGATCAGGTTCCTCAGAGAGAAACGGTGGTTCGTGCTACTGGTGGTGGCCGTCGTATGCGCCGGAGACCTGCTATGGCTGCTAGTTCGTCGAATGCAGAGGCAAGTCTGGAAGATAATGCTGATGGAAGTGATGATGAAATTGTGGCTGAAGAATACCATGGAGGAAGAGTGTTGAAGAAGAAAGAAAAGAAGCGACAAGAGCGGGAGGCACAAAGACAGGCTGAACAAGCTTCACGTGACTCAAGGTTGACAAAACAAGATCGTTATGCAGAAATACGGAGGAAAAAAGATGAGGAGCGTGAAGAGCAGGAACGGTTACTGGAAGAAGAAGCCAAGGCACGGAAGGCCAAGGAGGAAGAGGCTGCTGCACTTGAGTTTGAGAAGTGGAAAGGGGCATTTTCAGTTGACGCTGAAGGTACAACTGAAGCTGAAGTGGAAGGCGGAAATCAAGATTTGGTGACTGCTTTTGTGGAATATATAAAGAGTCATAAATGTGTCCCATTGGAAGATCTTGCTGCAGAATTTAAATTAAGAACTCAGGAATGTATTAATCGGATCACCTCCCTGGAGAGTATGGGACGACTTTCTGGCGTTATGGATGATAGAGGAAAATACATATACATATCAAAGGAAGAAATGCAAGCGGTAGCTGATTTCATCAAGCGTCAGGGAAGAGTTAGCATCTCACATCTAGCTAGTAAGTCCAACCAGTTCATTGATTTGGAACCAAAACTCCAGTTGGTTGAGGAAATCAATGTTGAGGAGATAGCTGTCTCATGA

Protein sequence

MEEFLVAVLSMLLVAALVPLFLWRRYQDSRSGHAHEDEDQVPQRETVVRATGGGRRMRRRPAMAASSSNAEASLEDNADGSDDEIVAEEYHGGRVLKKKEKKRQEREAQRQAEQASRDSRLTKQDRYAEIRRKKDEEREEQERLLEEEAKARKAKEEEAAALEFEKWKGAFSVDAEGTTEAEVEGGNQDLVTAFVEYIKSHKCVPLEDLAAEFKLRTQECINRITSLESMGRLSGVMDDRGKYIYISKEEMQAVADFIKRQGRVSISHLASKSNQFIDLEPKLQLVEEINVEEIAVS*
Homology
BLAST of CSPI03G23430 vs. ExPASy Swiss-Prot
Match: Q94C53 (DDRGK domain-containing protein 1 OS=Arabidopsis thaliana OX=3702 GN=At4g27120 PE=2 SV=1)

HSP 1 Score: 355.9 bits (912), Expect = 4.4e-97
Identity = 213/303 (70.30%), Postives = 250/303 (82.51%), Query Frame = 0

Query: 1   MEEFLVAVLSMLLVAALVPLFLWRRYQDSRSGHAHEDEDQVPQRETVVRATGGGRRMRRR 60
           MEE    ++SM+L+ A++PLF W+R +D+RS     +  QV  RE V RA GGGRRMRRR
Sbjct: 1   MEEIFALIVSMILIVAVIPLFFWKRRRDARSREEVAEPPQVQPRENVARA-GGGRRMRRR 60

Query: 61  PAMAASSSNAEASLEDNADGSDDEIVAEEYHGG---RVLKKKEKKRQEREAQRQAEQASR 120
           PA A+ +S++ +++++N  GS+DE   E+  GG   R  KKKEKKRQEREAQRQAE+A+R
Sbjct: 61  PA-ASGASSSTSNVQENGSGSEDE--DEDEAGGTQARASKKKEKKRQEREAQRQAEEATR 120

Query: 121 DSRLTKQDRYAEIRRKKDEEREEQERLLEEEAKARKAKEEEAAALEFEKWKGAFSVDAEG 180
           +SR TKQD YAE+RRKKDEERE +E  LEEE KAR+AKEEEAAALEF+KWKG FSVDAEG
Sbjct: 121 ESRNTKQDWYAEMRRKKDEEREAEELKLEEEEKARQAKEEEAAALEFDKWKGEFSVDAEG 180

Query: 181 TTEAEVEGGNQDLVTAFVEYIKSHKCVPLEDLAAEFKLRTQECINRITSLESMGRLSGVM 240
           TTE EV+GGNQDL++ FVEYIK  KCVPLEDLAAEF LRTQECINRI SLES+GRLSGVM
Sbjct: 181 TTE-EVQGGNQDLLSEFVEYIKKQKCVPLEDLAAEFHLRTQECINRIASLESIGRLSGVM 240

Query: 241 DDRGKYIYISKEEMQAVADFIKRQGRVSISHLASKSNQFIDLEPKL--QLVEEIN-VEEI 298
           DDRGKYIYIS EEM AVAD+IKRQGRVSISHLASKSNQFIDLEPK+  QL EEI+ +EEI
Sbjct: 241 DDRGKYIYISMEEMNAVADYIKRQGRVSISHLASKSNQFIDLEPKVQHQLTEEISGMEEI 298

BLAST of CSPI03G23430 vs. ExPASy Swiss-Prot
Match: Q8LH03 (DDRGK domain-containing protein 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os07g0103200 PE=2 SV=1)

HSP 1 Score: 318.9 bits (816), Expect = 6.0e-86
Identity = 195/297 (65.66%), Postives = 231/297 (77.78%), Query Frame = 0

Query: 5   LVAVLSMLLVAALVPLFLWRRYQDSRSGHAHEDEDQVPQRETVVR---ATG-GGRRMRRR 64
           L AV+ +LLV A+ PL LWRR    RS  AH    Q  Q E V+R   A G   RRMRRR
Sbjct: 8   LGAVVCLLLVFAIFPLLLWRR----RSDAAHRLPPQPLQDERVLRGGPAPGPAARRMRRR 67

Query: 65  PAMAASSSNAEASLEDNADGSDDEIVAEEYHGGRVLKKKEKKRQEREAQRQAEQASRDSR 124
           P   ++S++A  S + + D +D ++  E     R  KKKEKKRQ+REAQRQAE+A+RDSR
Sbjct: 68  P--LSTSADASTSRDRDVDDADSDLEEEIQDVPRGSKKKEKKRQDREAQRQAEEAARDSR 127

Query: 125 LTKQDRYAEIRRKKDEEREEQERLLEEEAKARKAKEEEAAALEFEKWKGAFSVDAEGTTE 184
            TKQDRYAE+RRKKDEERE QERL+EEEA+ARKAKEEEAAALEFEKWKGAFSVDAEGTTE
Sbjct: 128 RTKQDRYAEMRRKKDEEREAQERLMEEEARARKAKEEEAAALEFEKWKGAFSVDAEGTTE 187

Query: 185 AEVEGGNQDLVTAFVEYIKSHKCVPLEDLAAEFKLRTQECINRITSLESMGRLSGVMDDR 244
           ++ +   Q L+  FVEYIK+ KCVPLEDLAAEF++RTQ+CINRI +LE M RLSGVMDDR
Sbjct: 188 SDTQDDGQGLLHNFVEYIKNQKCVPLEDLAAEFRMRTQDCINRIITLEGMDRLSGVMDDR 247

Query: 245 GKYIYISKEEMQAVADFIKRQGRVSISHLASKSNQFIDLEPKLQLVEEINVEEIAVS 298
           GK+IYIS EEM+AVAD+I++QGRVSISHLAS SNQFIDLEPK Q  EE N++E A +
Sbjct: 248 GKFIYISTEEMKAVADYIRKQGRVSISHLASNSNQFIDLEPKPQYNEESNLDENAAA 298

BLAST of CSPI03G23430 vs. ExPASy Swiss-Prot
Match: B3S3D5 (DDRGK domain-containing protein 1 OS=Trichoplax adhaerens OX=10228 GN=TRIADDRAFT_58679 PE=3 SV=1)

HSP 1 Score: 147.9 bits (372), Expect = 1.8e-34
Identity = 121/284 (42.61%), Postives = 175/284 (61.62%), Query Frame = 0

Query: 5   LVAVLSMLLVAALVPLFLWRRYQ---DSRSGHAHEDEDQVPQRETVVRATGGGRRMRRRP 64
           + AV S+LL  A+  L    + +   D   G     E+ VPQR    RA  G R    R 
Sbjct: 9   IAAVASILLFVAVKLLSTDTKTEIRTDDDVGELIGRENPVPQRPR-ARARRGLRNKTNRS 68

Query: 65  AMAASSSNAEASLED--NADGSDDEIVAEEYHGGRVLKKKEKKRQEREAQRQAEQASRDS 124
                  N     +D    DG  +E V +   G + L+K E+K  E++A R+  +A R+ 
Sbjct: 69  KTQRQHDNDYDDYDDEYQDDGFLEEPVYDRKMGTKKLRKLEEK-AEKKAMREQMEAERED 128

Query: 125 RLTKQDRYAEIRRKKDEEREEQERLLEEEAKARKAKEEEAAALEFEKW---KGAFSVDAE 184
           ++ +Q+   E  R+K+EER+ +ER  +EEA+ RK KEE+    E+E++   K +FSV+ E
Sbjct: 129 KINRQE-LREQNRRKEEERKAKEREEKEEAE-RKLKEEQEKR-EYEEYLRLKESFSVENE 188

Query: 185 GTTEAEVEGGNQDLVTAFVEYIKSHKCVPLEDLAAEFKLRTQECINRITSLESMGRLSGV 244
           G+ EAE+   +Q L+  F++YIK++K V LED+AA FKLRT++ INRI +L+SM RL+GV
Sbjct: 189 GSGEAEIAQESQSLLQEFIDYIKNNKVVLLEDVAAHFKLRTEDVINRIQTLQSMDRLTGV 248

Query: 245 MDDRGKYIYISKEEMQAVADFIKRQGRVSISHLASKSNQFIDLE 281
           +DDRGKYIYIS EE+ A+A FIK++GRVSIS LA  SN  I+L+
Sbjct: 249 VDDRGKYIYISVEELNAIAKFIKQRGRVSISELAESSNSLINLQ 287

BLAST of CSPI03G23430 vs. ExPASy Swiss-Prot
Match: B0WIW5 (DDRGK domain-containing protein 1 OS=Culex quinquefasciatus OX=7176 GN=CPIJ007479 PE=3 SV=1)

HSP 1 Score: 140.2 bits (352), Expect = 3.8e-32
Identity = 116/292 (39.73%), Postives = 178/292 (60.96%), Query Frame = 0

Query: 1   MEEFLVAVLSMLLVAALVPLFLWRRYQDSRSGHAH-----EDEDQ-VPQRETVVRATGGG 60
           M+  L+  +++ L+  LV LF + + + S+    +     E+E Q  P+R  VVR     
Sbjct: 1   MDLILLLGIAVALLVILVTLFFFTKGKGSQESGKYNHPRVENEAQAAPRRAQVVR----N 60

Query: 61  RRMRRRPAMAASSSNAEASLEDNADGSDDEIVAEEYHGGRVLKKKEKK---RQEREAQRQ 120
           +R R R A A +     A+ +  +D  +DEI   +++G ++  KK  K   + E++A R+
Sbjct: 61  QRNRARVAAAPAEEQHHAAADGGSD--EDEIPHADFNGEKMGAKKRAKLEAKAEKKALRE 120

Query: 121 AEQASRDSRLTKQDRYAEIRRKKDEEREEQERLLEEEAKARKAKEEEAAA--LEFEKWKG 180
            E   R+ +  K+D   E +RK + E+E +E    EEA+ +KA+EE+A     E+ + K 
Sbjct: 121 QELKIREDQ-KKKDALLEEQRKVEAEKEAEEERKREEAE-KKAREEKARQEHEEYLRMKE 180

Query: 181 AFSVDAEGTTEAEVEGGNQDLVTAFVEYIKSHKCVPLEDLAAEFKLRTQECINRITSLES 240
           AFSV+ EG  + E E   Q+++  F+ ++KS+K V LEDLA +FKL+TQ  I+RI  L+ 
Sbjct: 181 AFSVEEEG-FDQEQEDDKQNMLQEFINFVKSNKVVVLEDLAVQFKLKTQAAIDRIVELQK 240

Query: 241 MGRLSGVMDDRGKYIYISKEEMQAVADFIKRQGRVSISHLASKSNQFIDLEP 282
            GRLSGV+DDRGK+IYIS+EE+ AVA FIK++GRVSI+ LA  SN  I+L P
Sbjct: 241 DGRLSGVIDDRGKFIYISEEELNAVAKFIKQRGRVSITELAENSNNLINLVP 283

BLAST of CSPI03G23430 vs. ExPASy Swiss-Prot
Match: C3Y431 (DDRGK domain-containing protein 1 OS=Branchiostoma floridae OX=7739 GN=BRAFLDRAFT_114851 PE=3 SV=1)

HSP 1 Score: 137.5 bits (345), Expect = 2.5e-31
Identity = 117/291 (40.21%), Postives = 169/291 (58.08%), Query Frame = 0

Query: 5   LVAVLSMLLVAALVPLFLWRRYQDSRSGHAHEDEDQVPQRETVVRATGGGRRMR--RRPA 64
           L AV ++ +   L      R  Q+ +     +       R    RA G  RR+R  R+  
Sbjct: 7   LYAVAAVFVAVLLFLAVKLRGGQEQQPADDGQRRGAPRPRTDAERAAGAPRRVRGARQRL 66

Query: 65  MAASSSNAEASLEDNADGSDDEIVAEEYHGGRVLKKKEKK---RQEREAQRQAEQASRDS 124
            AA   N + S E ++D  +     +   G +V  KK KK   + E++A R+A +  R+ 
Sbjct: 67  QAARRINQQDS-EGDSDSDEGRPEIDIPEGMKVGAKKMKKLEMKAEKKAAREAAEQEREE 126

Query: 125 RLTKQDRYAEIRRKKDEERE--EQERLLEEEAKARKAKEEEAAA--LEFEKWKGAFSVDA 184
           R  ++++  E +RK++EE E  E+ER  EEE   RKAKEE+AA    E+   K AF+V+ 
Sbjct: 127 R-KQREKMLEEKRKQEEEEEKLEEERRAEEE---RKAKEEKAAREHEEYLMMKEAFTVEE 186

Query: 185 EGTTEAEVEGGNQDLVTAFVEYIKSHKCVPLEDLAAEFKLRTQECINRITSLESMGRLSG 244
           EG  E   E   ++L+  F+++IK+ K V LEDLAA+F +RTQ+CI R+  L   GRL+G
Sbjct: 187 EGHEEILDEDATENLLQDFIDHIKAVKVVLLEDLAAQFNMRTQDCIQRVQELREQGRLTG 246

Query: 245 VMDDRGKYIYISKEEMQAVADFIKRQGRVSISHLASKSNQFIDLEPKLQLV 287
           V+DDRGK+IYIS+EE+QAVA FIK++GRVSI+ L   SNQ I L P  + V
Sbjct: 247 VIDDRGKFIYISQEELQAVAKFIKQRGRVSITDLVESSNQLIHLIPDTEQV 292

BLAST of CSPI03G23430 vs. ExPASy TrEMBL
Match: A0A0A0L7V5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G423830 PE=4 SV=1)

HSP 1 Score: 539.7 bits (1389), Expect = 7.9e-150
Identity = 296/297 (99.66%), Postives = 297/297 (100.00%), Query Frame = 0

Query: 1   MEEFLVAVLSMLLVAALVPLFLWRRYQDSRSGHAHEDEDQVPQRETVVRATGGGRRMRRR 60
           MEEFLVAVLSMLLVAALVPLFLWRRYQDSRSGHAHEDEDQVPQRETVVRATGGGRRMRRR
Sbjct: 1   MEEFLVAVLSMLLVAALVPLFLWRRYQDSRSGHAHEDEDQVPQRETVVRATGGGRRMRRR 60

Query: 61  PAMAASSSNAEASLEDNADGSDDEIVAEEYHGGRVLKKKEKKRQEREAQRQAEQASRDSR 120
           PAMAASSSNAEASLEDNADGSDDEIVAEEYHGGRVLKKKEKKRQEREAQRQAEQASRDSR
Sbjct: 61  PAMAASSSNAEASLEDNADGSDDEIVAEEYHGGRVLKKKEKKRQEREAQRQAEQASRDSR 120

Query: 121 LTKQDRYAEIRRKKDEEREEQERLLEEEAKARKAKEEEAAALEFEKWKGAFSVDAEGTTE 180
           LTKQDRYAEIRRKKDEEREEQERLLEEEAKARKAKEEEAAALEFEKWKGAFSVDAEGTTE
Sbjct: 121 LTKQDRYAEIRRKKDEEREEQERLLEEEAKARKAKEEEAAALEFEKWKGAFSVDAEGTTE 180

Query: 181 AEVEGGNQDLVTAFVEYIKSHKCVPLEDLAAEFKLRTQECINRITSLESMGRLSGVMDDR 240
           AEVEGGNQDL+TAFVEYIKSHKCVPLEDLAAEFKLRTQECINRITSLESMGRLSGVMDDR
Sbjct: 181 AEVEGGNQDLLTAFVEYIKSHKCVPLEDLAAEFKLRTQECINRITSLESMGRLSGVMDDR 240

Query: 241 GKYIYISKEEMQAVADFIKRQGRVSISHLASKSNQFIDLEPKLQLVEEINVEEIAVS 298
           GKYIYISKEEMQAVADFIKRQGRVSISHLASKSNQFIDLEPKLQLVEEINVEEIAVS
Sbjct: 241 GKYIYISKEEMQAVADFIKRQGRVSISHLASKSNQFIDLEPKLQLVEEINVEEIAVS 297

BLAST of CSPI03G23430 vs. ExPASy TrEMBL
Match: A0A5A7UHY4 (DDRGK domain-containing protein 1 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold778G00030 PE=4 SV=1)

HSP 1 Score: 525.4 bits (1352), Expect = 1.5e-145
Identity = 289/297 (97.31%), Postives = 291/297 (97.98%), Query Frame = 0

Query: 1   MEEFLVAVLSMLLVAALVPLFLWRRYQDSRSGHAHEDEDQVPQRETVVRATGGGRRMRRR 60
           MEEFLVAVLSMLLV ALVPLFLWRRYQDSRSGHAHEDEDQVPQRETVVRATGGGRRMRRR
Sbjct: 1   MEEFLVAVLSMLLVVALVPLFLWRRYQDSRSGHAHEDEDQVPQRETVVRATGGGRRMRRR 60

Query: 61  PAMAASSSNAEASLEDNADGSDDEIVAEEYHGGRVLKKKEKKRQEREAQRQAEQASRDSR 120
           PAMAASSSNA A +EDNADGSDDEI AEEYHGGRVLKKKEKKRQEREAQRQAEQASRDSR
Sbjct: 61  PAMAASSSNAGAFVEDNADGSDDEIAAEEYHGGRVLKKKEKKRQEREAQRQAEQASRDSR 120

Query: 121 LTKQDRYAEIRRKKDEEREEQERLLEEEAKARKAKEEEAAALEFEKWKGAFSVDAEGTTE 180
           LTKQDRYAEIRRKKDEEREEQERLLEEEAKARKAKEEEAAALEFEKWKGAFSVDAEGTTE
Sbjct: 121 LTKQDRYAEIRRKKDEEREEQERLLEEEAKARKAKEEEAAALEFEKWKGAFSVDAEGTTE 180

Query: 181 AEVEGGNQDLVTAFVEYIKSHKCVPLEDLAAEFKLRTQECINRITSLESMGRLSGVMDDR 240
           AEVEGGNQDL+TAFVEYIKSHKCVPLEDLAAEFKLRTQECINRITSLESMGRLSGVMDDR
Sbjct: 181 AEVEGGNQDLLTAFVEYIKSHKCVPLEDLAAEFKLRTQECINRITSLESMGRLSGVMDDR 240

Query: 241 GKYIYISKEEMQAVADFIKRQGRVSISHLASKSNQFIDLEPKLQLVEEINVEEIAVS 298
           GKYIYISKEEMQAVADFIKRQGRVSISHLASKSNQFIDLEPK QL EEINVEEIAVS
Sbjct: 241 GKYIYISKEEMQAVADFIKRQGRVSISHLASKSNQFIDLEPKSQLGEEINVEEIAVS 297

BLAST of CSPI03G23430 vs. ExPASy TrEMBL
Match: A0A1S3C022 (DDRGK domain-containing protein 1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103494924 PE=4 SV=1)

HSP 1 Score: 525.4 bits (1352), Expect = 1.5e-145
Identity = 289/297 (97.31%), Postives = 291/297 (97.98%), Query Frame = 0

Query: 1   MEEFLVAVLSMLLVAALVPLFLWRRYQDSRSGHAHEDEDQVPQRETVVRATGGGRRMRRR 60
           MEEFLVAVLSMLLV ALVPLFLWRRYQDSRSGHAHEDEDQVPQRETVVRATGGGRRMRRR
Sbjct: 1   MEEFLVAVLSMLLVVALVPLFLWRRYQDSRSGHAHEDEDQVPQRETVVRATGGGRRMRRR 60

Query: 61  PAMAASSSNAEASLEDNADGSDDEIVAEEYHGGRVLKKKEKKRQEREAQRQAEQASRDSR 120
           PAMAASSSNA A +EDNADGSDDEI AEEYHGGRVLKKKEKKRQEREAQRQAEQASRDSR
Sbjct: 61  PAMAASSSNAGAFVEDNADGSDDEIAAEEYHGGRVLKKKEKKRQEREAQRQAEQASRDSR 120

Query: 121 LTKQDRYAEIRRKKDEEREEQERLLEEEAKARKAKEEEAAALEFEKWKGAFSVDAEGTTE 180
           LTKQDRYAEIRRKKDEEREEQERLLEEEAKARKAKEEEAAALEFEKWKGAFSVDAEGTTE
Sbjct: 121 LTKQDRYAEIRRKKDEEREEQERLLEEEAKARKAKEEEAAALEFEKWKGAFSVDAEGTTE 180

Query: 181 AEVEGGNQDLVTAFVEYIKSHKCVPLEDLAAEFKLRTQECINRITSLESMGRLSGVMDDR 240
           AEVEGGNQDL+TAFVEYIKSHKCVPLEDLAAEFKLRTQECINRITSLESMGRLSGVMDDR
Sbjct: 181 AEVEGGNQDLLTAFVEYIKSHKCVPLEDLAAEFKLRTQECINRITSLESMGRLSGVMDDR 240

Query: 241 GKYIYISKEEMQAVADFIKRQGRVSISHLASKSNQFIDLEPKLQLVEEINVEEIAVS 298
           GKYIYISKEEMQAVADFIKRQGRVSISHLASKSNQFIDLEPK QL EEINVEEIAVS
Sbjct: 241 GKYIYISKEEMQAVADFIKRQGRVSISHLASKSNQFIDLEPKSQLGEEINVEEIAVS 297

BLAST of CSPI03G23430 vs. ExPASy TrEMBL
Match: A0A6J1IUB7 (DDRGK domain-containing protein 1-like OS=Cucurbita maxima OX=3661 GN=LOC111480645 PE=4 SV=1)

HSP 1 Score: 495.4 bits (1274), Expect = 1.7e-136
Identity = 271/297 (91.25%), Postives = 284/297 (95.62%), Query Frame = 0

Query: 1   MEEFLVAVLSMLLVAALVPLFLWRRYQDSRSGHAHEDEDQVPQRETVVRATGGGRRMRRR 60
           MEE L+AVLSMLLVAALVPLFLWRRYQDSRS HAHEDEDQVPQRET VRA GGGRRMRRR
Sbjct: 1   MEEVLLAVLSMLLVAALVPLFLWRRYQDSRSAHAHEDEDQVPQRETAVRAAGGGRRMRRR 60

Query: 61  PAMAASSSNAEASLEDNADGSDDEIVAEEYHGGRVLKKKEKKRQEREAQRQAEQASRDSR 120
           PA AASSSNA ASLE++ADGSDDEI A+EYH GRV KKKEKKRQEREAQRQAEQASRDSR
Sbjct: 61  PASAASSSNAGASLEESADGSDDEIAADEYHDGRVSKKKEKKRQEREAQRQAEQASRDSR 120

Query: 121 LTKQDRYAEIRRKKDEEREEQERLLEEEAKARKAKEEEAAALEFEKWKGAFSVDAEGTTE 180
           +TK+DRY EIRRKKDEEREEQERLLEEEAKARKAKEEEAAALEFEKWKG FSVDAEGTTE
Sbjct: 121 VTKKDRYEEIRRKKDEEREEQERLLEEEAKARKAKEEEAAALEFEKWKGDFSVDAEGTTE 180

Query: 181 AEVEGGNQDLVTAFVEYIKSHKCVPLEDLAAEFKLRTQECINRITSLESMGRLSGVMDDR 240
           AEVEGGNQDLV+AFV+YIK HKCVPLEDLAAEFKLRTQECINRITSLESMGRLSGVMDDR
Sbjct: 181 AEVEGGNQDLVSAFVDYIKCHKCVPLEDLAAEFKLRTQECINRITSLESMGRLSGVMDDR 240

Query: 241 GKYIYISKEEMQAVADFIKRQGRVSISHLASKSNQFIDLEPKLQLVEEINVEEIAVS 298
           GKYIYISKEEMQAVA++I+RQGRVSISHLASKSNQFIDLEPK+QLVEE+NVEEI VS
Sbjct: 241 GKYIYISKEEMQAVAEYIQRQGRVSISHLASKSNQFIDLEPKVQLVEEMNVEEITVS 297

BLAST of CSPI03G23430 vs. ExPASy TrEMBL
Match: A0A5D3E1I9 (DDRGK domain-containing protein 1 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold588G00280 PE=4 SV=1)

HSP 1 Score: 495.0 bits (1273), Expect = 2.2e-136
Identity = 278/297 (93.60%), Postives = 284/297 (95.62%), Query Frame = 0

Query: 1   MEEFLVAVLSMLLVAALVPLFLWRRYQDSRSGHAHEDEDQVPQRETVVRATGGGRRMRRR 60
           MEEFLVAVLSMLLV ALVPLFLWRRYQDSRSGHAHEDEDQVPQRETVVRATGGGRRMRRR
Sbjct: 1   MEEFLVAVLSMLLVVALVPLFLWRRYQDSRSGHAHEDEDQVPQRETVVRATGGGRRMRRR 60

Query: 61  PAMAASSSNAEASLEDNADGSDDEIVAEEYHGGRVLKKKEKKRQEREAQRQAEQASRDSR 120
           PAMAASSSNA A +EDNADGSDDEI AEEYHGGRVLKKKEKKRQEREAQRQAEQASRDSR
Sbjct: 61  PAMAASSSNAGAFVEDNADGSDDEIAAEEYHGGRVLKKKEKKRQEREAQRQAEQASRDSR 120

Query: 121 LTKQDRYAEIRRKKDEEREEQERLLEEEAKARKAKEEEAAALEFEKWKGAFSVDAEGTTE 180
           LTKQDRYAEIRRKKDEEREEQERLLEEEAKARKAKEEEAAALEFEKWKGAFSVDAEGTTE
Sbjct: 121 LTKQDRYAEIRRKKDEEREEQERLLEEEAKARKAKEEEAAALEFEKWKGAFSVDAEGTTE 180

Query: 181 AEVEGGNQDLVTAFVEYIKSHKCVPLEDLAAEFKLRTQECINRITSLESMGRLSGVMDDR 240
           AEVEGGNQDL+TAFVEYIKSHKCVPLEDLAAEFKLRTQ  + +I  +  +GRLSGVMDDR
Sbjct: 181 AEVEGGNQDLLTAFVEYIKSHKCVPLEDLAAEFKLRTQ--VTQI--MVVVGRLSGVMDDR 240

Query: 241 GKYIYISKEEMQAVADFIKRQGRVSISHLASKSNQFIDLEPKLQLVEEINVEEIAVS 298
           GKYIYISKEEMQAVADFIKRQGRVSISHLASKSNQFIDLEPK QL EEINVEEIAVS
Sbjct: 241 GKYIYISKEEMQAVADFIKRQGRVSISHLASKSNQFIDLEPKSQLGEEINVEEIAVS 293

BLAST of CSPI03G23430 vs. NCBI nr
Match: XP_004151951.1 (DDRGK domain-containing protein 1 [Cucumis sativus] >XP_031737646.1 DDRGK domain-containing protein 1-like [Cucumis sativus] >XP_031737647.1 DDRGK domain-containing protein 1-like [Cucumis sativus] >XP_031745852.1 DDRGK domain-containing protein 1 [Cucumis sativus] >KAE8637504.1 hypothetical protein CSA_004760 [Cucumis sativus] >KGN57995.1 hypothetical protein Csa_010214 [Cucumis sativus])

HSP 1 Score: 539.7 bits (1389), Expect = 1.6e-149
Identity = 296/297 (99.66%), Postives = 297/297 (100.00%), Query Frame = 0

Query: 1   MEEFLVAVLSMLLVAALVPLFLWRRYQDSRSGHAHEDEDQVPQRETVVRATGGGRRMRRR 60
           MEEFLVAVLSMLLVAALVPLFLWRRYQDSRSGHAHEDEDQVPQRETVVRATGGGRRMRRR
Sbjct: 1   MEEFLVAVLSMLLVAALVPLFLWRRYQDSRSGHAHEDEDQVPQRETVVRATGGGRRMRRR 60

Query: 61  PAMAASSSNAEASLEDNADGSDDEIVAEEYHGGRVLKKKEKKRQEREAQRQAEQASRDSR 120
           PAMAASSSNAEASLEDNADGSDDEIVAEEYHGGRVLKKKEKKRQEREAQRQAEQASRDSR
Sbjct: 61  PAMAASSSNAEASLEDNADGSDDEIVAEEYHGGRVLKKKEKKRQEREAQRQAEQASRDSR 120

Query: 121 LTKQDRYAEIRRKKDEEREEQERLLEEEAKARKAKEEEAAALEFEKWKGAFSVDAEGTTE 180
           LTKQDRYAEIRRKKDEEREEQERLLEEEAKARKAKEEEAAALEFEKWKGAFSVDAEGTTE
Sbjct: 121 LTKQDRYAEIRRKKDEEREEQERLLEEEAKARKAKEEEAAALEFEKWKGAFSVDAEGTTE 180

Query: 181 AEVEGGNQDLVTAFVEYIKSHKCVPLEDLAAEFKLRTQECINRITSLESMGRLSGVMDDR 240
           AEVEGGNQDL+TAFVEYIKSHKCVPLEDLAAEFKLRTQECINRITSLESMGRLSGVMDDR
Sbjct: 181 AEVEGGNQDLLTAFVEYIKSHKCVPLEDLAAEFKLRTQECINRITSLESMGRLSGVMDDR 240

Query: 241 GKYIYISKEEMQAVADFIKRQGRVSISHLASKSNQFIDLEPKLQLVEEINVEEIAVS 298
           GKYIYISKEEMQAVADFIKRQGRVSISHLASKSNQFIDLEPKLQLVEEINVEEIAVS
Sbjct: 241 GKYIYISKEEMQAVADFIKRQGRVSISHLASKSNQFIDLEPKLQLVEEINVEEIAVS 297

BLAST of CSPI03G23430 vs. NCBI nr
Match: XP_008454532.1 (PREDICTED: DDRGK domain-containing protein 1 isoform X1 [Cucumis melo] >KAA0053079.1 DDRGK domain-containing protein 1 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 525.4 bits (1352), Expect = 3.2e-145
Identity = 289/297 (97.31%), Postives = 291/297 (97.98%), Query Frame = 0

Query: 1   MEEFLVAVLSMLLVAALVPLFLWRRYQDSRSGHAHEDEDQVPQRETVVRATGGGRRMRRR 60
           MEEFLVAVLSMLLV ALVPLFLWRRYQDSRSGHAHEDEDQVPQRETVVRATGGGRRMRRR
Sbjct: 1   MEEFLVAVLSMLLVVALVPLFLWRRYQDSRSGHAHEDEDQVPQRETVVRATGGGRRMRRR 60

Query: 61  PAMAASSSNAEASLEDNADGSDDEIVAEEYHGGRVLKKKEKKRQEREAQRQAEQASRDSR 120
           PAMAASSSNA A +EDNADGSDDEI AEEYHGGRVLKKKEKKRQEREAQRQAEQASRDSR
Sbjct: 61  PAMAASSSNAGAFVEDNADGSDDEIAAEEYHGGRVLKKKEKKRQEREAQRQAEQASRDSR 120

Query: 121 LTKQDRYAEIRRKKDEEREEQERLLEEEAKARKAKEEEAAALEFEKWKGAFSVDAEGTTE 180
           LTKQDRYAEIRRKKDEEREEQERLLEEEAKARKAKEEEAAALEFEKWKGAFSVDAEGTTE
Sbjct: 121 LTKQDRYAEIRRKKDEEREEQERLLEEEAKARKAKEEEAAALEFEKWKGAFSVDAEGTTE 180

Query: 181 AEVEGGNQDLVTAFVEYIKSHKCVPLEDLAAEFKLRTQECINRITSLESMGRLSGVMDDR 240
           AEVEGGNQDL+TAFVEYIKSHKCVPLEDLAAEFKLRTQECINRITSLESMGRLSGVMDDR
Sbjct: 181 AEVEGGNQDLLTAFVEYIKSHKCVPLEDLAAEFKLRTQECINRITSLESMGRLSGVMDDR 240

Query: 241 GKYIYISKEEMQAVADFIKRQGRVSISHLASKSNQFIDLEPKLQLVEEINVEEIAVS 298
           GKYIYISKEEMQAVADFIKRQGRVSISHLASKSNQFIDLEPK QL EEINVEEIAVS
Sbjct: 241 GKYIYISKEEMQAVADFIKRQGRVSISHLASKSNQFIDLEPKSQLGEEINVEEIAVS 297

BLAST of CSPI03G23430 vs. NCBI nr
Match: XP_038896745.1 (DDRGK domain-containing protein 1 [Benincasa hispida] >XP_038896746.1 DDRGK domain-containing protein 1 [Benincasa hispida])

HSP 1 Score: 508.8 bits (1309), Expect = 3.1e-140
Identity = 280/297 (94.28%), Postives = 285/297 (95.96%), Query Frame = 0

Query: 1   MEEFLVAVLSMLLVAALVPLFLWRRYQDSRSGHAHEDEDQVPQRETVVRATGGGRRMRRR 60
           MEEFLVAVLSMLLV ALVPLFLWRRYQDSRSGHAHEDEDQVP+RETVVRATGGGRRMRRR
Sbjct: 1   MEEFLVAVLSMLLVVALVPLFLWRRYQDSRSGHAHEDEDQVPRRETVVRATGGGRRMRRR 60

Query: 61  PAMAASSSNAEASLEDNADGSDDEIVAEEYHGGRVLKKKEKKRQEREAQRQAEQASRDSR 120
           P  AASSSNA ASLEDN DGSDDEI AEEY  GRVLKKKEKKRQEREAQRQAEQASRDSR
Sbjct: 61  PTTAASSSNAGASLEDNGDGSDDEIAAEEYPEGRVLKKKEKKRQEREAQRQAEQASRDSR 120

Query: 121 LTKQDRYAEIRRKKDEEREEQERLLEEEAKARKAKEEEAAALEFEKWKGAFSVDAEGTTE 180
           LTKQDRYAE+RRKKDEEREEQERLLEEEAKARKAKEEEAAA EFEKWKG FSVDAEGTTE
Sbjct: 121 LTKQDRYAEMRRKKDEEREEQERLLEEEAKARKAKEEEAAAFEFEKWKGEFSVDAEGTTE 180

Query: 181 AEVEGGNQDLVTAFVEYIKSHKCVPLEDLAAEFKLRTQECINRITSLESMGRLSGVMDDR 240
           AEVEGGNQDL++AFVEYIKSHKCVPLEDLAAEFKLRTQECINRI+SLESMGRLSGVMDDR
Sbjct: 181 AEVEGGNQDLLSAFVEYIKSHKCVPLEDLAAEFKLRTQECINRISSLESMGRLSGVMDDR 240

Query: 241 GKYIYISKEEMQAVADFIKRQGRVSISHLASKSNQFIDLEPKLQLVEEINVEEIAVS 298
           GKYIYISKEEMQAVADFIKRQGRVSISHLASKSNQFIDLEPK QL EEINVEEIAVS
Sbjct: 241 GKYIYISKEEMQAVADFIKRQGRVSISHLASKSNQFIDLEPKAQLAEEINVEEIAVS 297

BLAST of CSPI03G23430 vs. NCBI nr
Match: XP_022981566.1 (DDRGK domain-containing protein 1-like [Cucurbita maxima] >XP_022981567.1 DDRGK domain-containing protein 1-like [Cucurbita maxima])

HSP 1 Score: 495.4 bits (1274), Expect = 3.5e-136
Identity = 271/297 (91.25%), Postives = 284/297 (95.62%), Query Frame = 0

Query: 1   MEEFLVAVLSMLLVAALVPLFLWRRYQDSRSGHAHEDEDQVPQRETVVRATGGGRRMRRR 60
           MEE L+AVLSMLLVAALVPLFLWRRYQDSRS HAHEDEDQVPQRET VRA GGGRRMRRR
Sbjct: 1   MEEVLLAVLSMLLVAALVPLFLWRRYQDSRSAHAHEDEDQVPQRETAVRAAGGGRRMRRR 60

Query: 61  PAMAASSSNAEASLEDNADGSDDEIVAEEYHGGRVLKKKEKKRQEREAQRQAEQASRDSR 120
           PA AASSSNA ASLE++ADGSDDEI A+EYH GRV KKKEKKRQEREAQRQAEQASRDSR
Sbjct: 61  PASAASSSNAGASLEESADGSDDEIAADEYHDGRVSKKKEKKRQEREAQRQAEQASRDSR 120

Query: 121 LTKQDRYAEIRRKKDEEREEQERLLEEEAKARKAKEEEAAALEFEKWKGAFSVDAEGTTE 180
           +TK+DRY EIRRKKDEEREEQERLLEEEAKARKAKEEEAAALEFEKWKG FSVDAEGTTE
Sbjct: 121 VTKKDRYEEIRRKKDEEREEQERLLEEEAKARKAKEEEAAALEFEKWKGDFSVDAEGTTE 180

Query: 181 AEVEGGNQDLVTAFVEYIKSHKCVPLEDLAAEFKLRTQECINRITSLESMGRLSGVMDDR 240
           AEVEGGNQDLV+AFV+YIK HKCVPLEDLAAEFKLRTQECINRITSLESMGRLSGVMDDR
Sbjct: 181 AEVEGGNQDLVSAFVDYIKCHKCVPLEDLAAEFKLRTQECINRITSLESMGRLSGVMDDR 240

Query: 241 GKYIYISKEEMQAVADFIKRQGRVSISHLASKSNQFIDLEPKLQLVEEINVEEIAVS 298
           GKYIYISKEEMQAVA++I+RQGRVSISHLASKSNQFIDLEPK+QLVEE+NVEEI VS
Sbjct: 241 GKYIYISKEEMQAVAEYIQRQGRVSISHLASKSNQFIDLEPKVQLVEEMNVEEITVS 297

BLAST of CSPI03G23430 vs. NCBI nr
Match: TYK29963.1 (DDRGK domain-containing protein 1 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 495.0 bits (1273), Expect = 4.6e-136
Identity = 278/297 (93.60%), Postives = 284/297 (95.62%), Query Frame = 0

Query: 1   MEEFLVAVLSMLLVAALVPLFLWRRYQDSRSGHAHEDEDQVPQRETVVRATGGGRRMRRR 60
           MEEFLVAVLSMLLV ALVPLFLWRRYQDSRSGHAHEDEDQVPQRETVVRATGGGRRMRRR
Sbjct: 1   MEEFLVAVLSMLLVVALVPLFLWRRYQDSRSGHAHEDEDQVPQRETVVRATGGGRRMRRR 60

Query: 61  PAMAASSSNAEASLEDNADGSDDEIVAEEYHGGRVLKKKEKKRQEREAQRQAEQASRDSR 120
           PAMAASSSNA A +EDNADGSDDEI AEEYHGGRVLKKKEKKRQEREAQRQAEQASRDSR
Sbjct: 61  PAMAASSSNAGAFVEDNADGSDDEIAAEEYHGGRVLKKKEKKRQEREAQRQAEQASRDSR 120

Query: 121 LTKQDRYAEIRRKKDEEREEQERLLEEEAKARKAKEEEAAALEFEKWKGAFSVDAEGTTE 180
           LTKQDRYAEIRRKKDEEREEQERLLEEEAKARKAKEEEAAALEFEKWKGAFSVDAEGTTE
Sbjct: 121 LTKQDRYAEIRRKKDEEREEQERLLEEEAKARKAKEEEAAALEFEKWKGAFSVDAEGTTE 180

Query: 181 AEVEGGNQDLVTAFVEYIKSHKCVPLEDLAAEFKLRTQECINRITSLESMGRLSGVMDDR 240
           AEVEGGNQDL+TAFVEYIKSHKCVPLEDLAAEFKLRTQ  + +I  +  +GRLSGVMDDR
Sbjct: 181 AEVEGGNQDLLTAFVEYIKSHKCVPLEDLAAEFKLRTQ--VTQI--MVVVGRLSGVMDDR 240

Query: 241 GKYIYISKEEMQAVADFIKRQGRVSISHLASKSNQFIDLEPKLQLVEEINVEEIAVS 298
           GKYIYISKEEMQAVADFIKRQGRVSISHLASKSNQFIDLEPK QL EEINVEEIAVS
Sbjct: 241 GKYIYISKEEMQAVADFIKRQGRVSISHLASKSNQFIDLEPKSQLGEEINVEEIAVS 293

BLAST of CSPI03G23430 vs. TAIR 10
Match: AT4G27120.1 (CONTAINS InterPro DOMAIN/s: DDRGK domain (InterPro:IPR019153); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 355.9 bits (912), Expect = 3.1e-98
Identity = 213/303 (70.30%), Postives = 250/303 (82.51%), Query Frame = 0

Query: 1   MEEFLVAVLSMLLVAALVPLFLWRRYQDSRSGHAHEDEDQVPQRETVVRATGGGRRMRRR 60
           MEE    ++SM+L+ A++PLF W+R +D+RS     +  QV  RE V RA GGGRRMRRR
Sbjct: 1   MEEIFALIVSMILIVAVIPLFFWKRRRDARSREEVAEPPQVQPRENVARA-GGGRRMRRR 60

Query: 61  PAMAASSSNAEASLEDNADGSDDEIVAEEYHGG---RVLKKKEKKRQEREAQRQAEQASR 120
           PA A+ +S++ +++++N  GS+DE   E+  GG   R  KKKEKKRQEREAQRQAE+A+R
Sbjct: 61  PA-ASGASSSTSNVQENGSGSEDE--DEDEAGGTQARASKKKEKKRQEREAQRQAEEATR 120

Query: 121 DSRLTKQDRYAEIRRKKDEEREEQERLLEEEAKARKAKEEEAAALEFEKWKGAFSVDAEG 180
           +SR TKQD YAE+RRKKDEERE +E  LEEE KAR+AKEEEAAALEF+KWKG FSVDAEG
Sbjct: 121 ESRNTKQDWYAEMRRKKDEEREAEELKLEEEEKARQAKEEEAAALEFDKWKGEFSVDAEG 180

Query: 181 TTEAEVEGGNQDLVTAFVEYIKSHKCVPLEDLAAEFKLRTQECINRITSLESMGRLSGVM 240
           TTE EV+GGNQDL++ FVEYIK  KCVPLEDLAAEF LRTQECINRI SLES+GRLSGVM
Sbjct: 181 TTE-EVQGGNQDLLSEFVEYIKKQKCVPLEDLAAEFHLRTQECINRIASLESIGRLSGVM 240

Query: 241 DDRGKYIYISKEEMQAVADFIKRQGRVSISHLASKSNQFIDLEPKL--QLVEEIN-VEEI 298
           DDRGKYIYIS EEM AVAD+IKRQGRVSISHLASKSNQFIDLEPK+  QL EEI+ +EEI
Sbjct: 241 DDRGKYIYISMEEMNAVADYIKRQGRVSISHLASKSNQFIDLEPKVQHQLTEEISGMEEI 298

BLAST of CSPI03G23430 vs. TAIR 10
Match: AT4G27120.2 (CONTAINS InterPro DOMAIN/s: DDRGK domain (InterPro:IPR019153); Has 14775 Blast hits to 8764 proteins in 778 species: Archae - 29; Bacteria - 1878; Metazoa - 5164; Fungi - 1447; Plants - 582; Viruses - 164; Other Eukaryotes - 5511 (source: NCBI BLink). )

HSP 1 Score: 355.9 bits (912), Expect = 3.1e-98
Identity = 213/303 (70.30%), Postives = 250/303 (82.51%), Query Frame = 0

Query: 1   MEEFLVAVLSMLLVAALVPLFLWRRYQDSRSGHAHEDEDQVPQRETVVRATGGGRRMRRR 60
           MEE    ++SM+L+ A++PLF W+R +D+RS     +  QV  RE V RA GGGRRMRRR
Sbjct: 1   MEEIFALIVSMILIVAVIPLFFWKRRRDARSREEVAEPPQVQPRENVARA-GGGRRMRRR 60

Query: 61  PAMAASSSNAEASLEDNADGSDDEIVAEEYHGG---RVLKKKEKKRQEREAQRQAEQASR 120
           PA A+ +S++ +++++N  GS+DE   E+  GG   R  KKKEKKRQEREAQRQAE+A+R
Sbjct: 61  PA-ASGASSSTSNVQENGSGSEDE--DEDEAGGTQARASKKKEKKRQEREAQRQAEEATR 120

Query: 121 DSRLTKQDRYAEIRRKKDEEREEQERLLEEEAKARKAKEEEAAALEFEKWKGAFSVDAEG 180
           +SR TKQD YAE+RRKKDEERE +E  LEEE KAR+AKEEEAAALEF+KWKG FSVDAEG
Sbjct: 121 ESRNTKQDWYAEMRRKKDEEREAEELKLEEEEKARQAKEEEAAALEFDKWKGEFSVDAEG 180

Query: 181 TTEAEVEGGNQDLVTAFVEYIKSHKCVPLEDLAAEFKLRTQECINRITSLESMGRLSGVM 240
           TTE EV+GGNQDL++ FVEYIK  KCVPLEDLAAEF LRTQECINRI SLES+GRLSGVM
Sbjct: 181 TTE-EVQGGNQDLLSEFVEYIKKQKCVPLEDLAAEFHLRTQECINRIASLESIGRLSGVM 240

Query: 241 DDRGKYIYISKEEMQAVADFIKRQGRVSISHLASKSNQFIDLEPKL--QLVEEIN-VEEI 298
           DDRGKYIYIS EEM AVAD+IKRQGRVSISHLASKSNQFIDLEPK+  QL EEI+ +EEI
Sbjct: 241 DDRGKYIYISMEEMNAVADYIKRQGRVSISHLASKSNQFIDLEPKVQHQLTEEISGMEEI 298

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q94C534.4e-9770.30DDRGK domain-containing protein 1 OS=Arabidopsis thaliana OX=3702 GN=At4g27120 P... [more]
Q8LH036.0e-8665.66DDRGK domain-containing protein 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os... [more]
B3S3D51.8e-3442.61DDRGK domain-containing protein 1 OS=Trichoplax adhaerens OX=10228 GN=TRIADDRAFT... [more]
B0WIW53.8e-3239.73DDRGK domain-containing protein 1 OS=Culex quinquefasciatus OX=7176 GN=CPIJ00747... [more]
C3Y4312.5e-3140.21DDRGK domain-containing protein 1 OS=Branchiostoma floridae OX=7739 GN=BRAFLDRAF... [more]
Match NameE-valueIdentityDescription
A0A0A0L7V57.9e-15099.66Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G423830 PE=4 SV=1[more]
A0A5A7UHY41.5e-14597.31DDRGK domain-containing protein 1 isoform X1 OS=Cucumis melo var. makuwa OX=1194... [more]
A0A1S3C0221.5e-14597.31DDRGK domain-containing protein 1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10349... [more]
A0A6J1IUB71.7e-13691.25DDRGK domain-containing protein 1-like OS=Cucurbita maxima OX=3661 GN=LOC1114806... [more]
A0A5D3E1I92.2e-13693.60DDRGK domain-containing protein 1 isoform X1 OS=Cucumis melo var. makuwa OX=1194... [more]
Match NameE-valueIdentityDescription
XP_004151951.11.6e-14999.66DDRGK domain-containing protein 1 [Cucumis sativus] >XP_031737646.1 DDRGK domain... [more]
XP_008454532.13.2e-14597.31PREDICTED: DDRGK domain-containing protein 1 isoform X1 [Cucumis melo] >KAA00530... [more]
XP_038896745.13.1e-14094.28DDRGK domain-containing protein 1 [Benincasa hispida] >XP_038896746.1 DDRGK doma... [more]
XP_022981566.13.5e-13691.25DDRGK domain-containing protein 1-like [Cucurbita maxima] >XP_022981567.1 DDRGK ... [more]
TYK29963.14.6e-13693.60DDRGK domain-containing protein 1 isoform X1 [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
AT4G27120.13.1e-9870.30CONTAINS InterPro DOMAIN/s: DDRGK domain (InterPro:IPR019153); Has 30201 Blast h... [more]
AT4G27120.23.1e-9870.30CONTAINS InterPro DOMAIN/s: DDRGK domain (InterPro:IPR019153); Has 14775 Blast h... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 120..165
NoneNo IPR availableCOILSCoilCoilcoord: 94..118
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 84..139
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 32..139
NoneNo IPR availablePANTHERPTHR48176DDRGK DOMAIN-CONTAINING PROTEIN 1coord: 71..232
IPR019153DDRGK domain containing proteinSMARTSM01128DDRGK_2coord: 93..246
e-value: 6.3E-31
score: 118.7
IPR019153DDRGK domain containing proteinPFAMPF09756DDRGKcoord: 96..232
e-value: 1.2E-26
score: 93.4
IPR036388Winged helix-like DNA-binding domain superfamilyGENE3D1.10.10.10coord: 189..234
e-value: 2.5E-15
score: 58.3
IPR036390Winged helix DNA-binding domain superfamilySUPERFAMILY46785"Winged helix" DNA-binding domaincoord: 185..232

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI03G23430.1CSPI03G23430.1mRNA
CSPI03G23430.2CSPI03G23430.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0008168 methyltransferase activity